1 Volume 23 2024 e240338 Original Article Braz J Oral Sci. 2024;23:e240338http://dx.doi.org/10.20396/bjos.v23i00.8670338 1 Former Post Graduate Student, Department of Periodontics, JMF’s ACPM Dental college, Sakri Road, Dhule, Maharashtra, India. 2 Professor, Department of Periodontics, JMF’s ACPM Dental college, Sakri Road, Dhule, Maharashtra, India. Corresponding author: Anuradha Bhatsange Professor, Dept. of Periodontics, JMF’s ACPM Dental college JMF’s ACPM dental college, Sakri Road, Dhule, Maharashtra, India, 424001. Phone numbers: 9960881275; E-mail: anujaps219@gmail.com Editor: Altair A. Del Bel Cury Received: July 15, 2022 Accepted: June 02, 2023 Demineralized freeze-dried bone allograft with/without i-Platelet-rich fibrin in 3 wall intrabony defects Rinnu Mathew1 , Anuradha Bhatsange2* Demineralized freeze-dried bone allograft (DFDBA) contains bone morphogenetic proteins (BMPs), hence is osteoinductive. Autologous platelet concentrates exhibit a higher quantity of growth factors. Both these biomaterials aid in bone regeneration when placed in three-wall intrabony defects. However, their efficacy when used alone and in conjugation is not clear. Aim: To assess clinical and radiographic efficacy of injectable platelet-rich fibrin (i-PRF) with microsurgical access flap in the treatment of three-wall intrabony defects in chronic periodontitis patients. Methods: Thirty sites with three-wall intrabony defects were randomly assigned to control and test group by computer generated method. The test group obtained i-PRF mixed with DFDBA while the control group received only DFDBA. Clinical parameters such as site-specific Plaque index (PI), Radiographic intrabony defect depth (IBDD), modified- Sulcular bleeding index (mSBI), Clinical attachment level (CAL), and Probing pocket depth (PPD) were measured at baseline, three and six months. Results: Intragroup comparison within the control group and test group exhibited statistically highly significant variation of mean PI, mSBI, PPD, CAL, and IBDD score from baseline to 3 months and from 3-6 months (p<0.001). However, intergroup comparison demonstrated no statistically significant variation of mean IBDD at all 3 intervals (p>0.05). Conclusion: i-PRF combined with DFDBA enhanced the radiographic and clinical parameters as opposed to DFDBA alone. The role of i-PRF is promising in its capacity for easy obtainability and increased potential to aid in regeneration. Keywords: Periodontitis. Allografts. Alveolar bone loss. Platelet- Rich Fibrin. Regeneration. https://orcid.org/0000-0002-6180-1827 https://orcid.org/0000-0001-8188-217X 2 Mathew et al. Braz J Oral Sci. 2024;23:e240338 Introduction Regeneration of periodontium is a process of restoring the complete architecture and function of the periodontium1. It involves new functional periodontal attach- ment between newly formed bone and cementum1,2. The process constitutes a multi-dependent sequence of biological events including cell adhesion, migration, proliferation, and differentiation3. Periodontitis is defined as an inflammatory disease of the supporting structural apparatus resulting in progressive destruction of the “periodontal ligament”, alve- olar bone with pocket formation, recession, or both. Bone loss that occurs as a consequence of this inflammatory disease results in different patterns and in vari- ous combinations. The vertical/ angular defects that occur in an oblique direction, with a hollow trough in the bone alongside the root with base located apical to the surrounding bone is termed as intrabony defect4. These defects when surrounded by two or three walls become more amenable to regeneration as it provides the best spatial relationship for defect bridging by providing vascular and cellular ele- ments from the periodontal ligament and adjacent osseous wall5. Although various attempts to accomplish goals of regeneration have been tried using bone grafts, [autografts, allografts, xenografts, alloplasts] membranes, polypeptide growth fac- tors, tissue engineering applications etc, reconstruction of fully functioning peri- odontium still remains a big challenge despite the advances. “Demineralized freeze- dried bone allograft” (DFDBA) a widely used allograft, exhibits osteoinductive and osteoconductive properties owing to its content of bone morphogenetic proteins2. These proteins orchestrate mesenchymal cell migration, new attachment, and osteogenesis that help to regenerate cementum, bone, and periodontal ligament with histological evidence6. However the amount of bone fill found with the use of DFDBA has not been very clear. Hence, in the quest to enhance the efficacy of peri- odontal regeneration with various bone grafts, polypeptide growth factors derived from the autologous blood concentrates in the form of “platelet-rich plasma” (PRP) and “platelet-rich fibrin” (PRF) have been used. These show the ability to induce cell proliferation and differentiation and osteoinduction. The initial biochemical analysis of PRF composition indicated it to be an intimate assembly of cytokines, glycanic chains, and structural glycoproteins enmeshed within a slowly polymerized fibrin network7. Later on the use of PRF membrane has been shown to exhibit a significant but, slow sustained release of key “growth factors” for ≥ 1 week and ≤ 28 days, sug- gesting its ability to stimulate its environment for wound healing8. However, another form of PRF, i.e. an injectable form of PRF (i-PRF) demonstrated good results with higher concentration of stem cells and better potential for regeneration due to its capacity of inducing higher cell migration and mRNA expression of “Transforming growth factor-beta” (TGF-β), “platelet derived growth factor” (PDGF), and collagen1. The property of i-PRF forming a hydrogel even after 10 days hypothesized it to release additional growth factors9. Good and predictable outcomes of regenerative procedures are accomplished by incorporation of microsurgery applications too. Microsurgery refers to refine- ment in surgical technique with vision enhancement through magnification and 3 Mathew et al. Braz J Oral Sci. 2024;23:e240338 was introduced to the speciality of Periodontics in 199210. Periodontal microsur- gery helps to: 1) improve tissue preservation and better handling of specific flap designs to access the defects; 2) optimize defect debridement and root instru- mentation; 3) ensure optimal delivery of the regenerative technology; 4) optimize flap mobility to achieve primary closure of the interdental space11. It’s application promises to change clinical concepts of periodontal surgical care by improve- ment in predictability, cosmetic result and patient comfort level over conventional periodontal surgical procedures12. This implies placement of accurately mapped incisions, elevation of flap with minimal damage, precise wound closure without tension, resulting in reduction of postoperative morbidity. Rendering maximum benefit to the patient with advancements in the treatment aspects, and the best regenerative material available, the current study was carried out to explore the clinical and radiographic effectiveness of i-PRF combined with DFDBA by micro- surgical access flap in the treatment of three-wall intrabony defects in patients with “chronic periodontitis”. Material and methods Trial design and ethics approval This was a randomized controlled clinico-radiographic study carried out in Dept. of Periodontology. A sample size of thirty sites were chosen to employ statistical soft- ware, projected by evaluating the prior literature performed in the field of research and maintaining the confidence interval at 95% with a relative precision of 20%. The study was approved by institutional review board. Participants Sixty-one patients were recruited from out-patient department and assessed for eligi- bility. Out of these 31 patients were excluded as they did not meet the inclusion crite- ria. Chronic periodontitis patients between 30-50 years of age with 30 sites exhibiting 3 wall intrabony defects>3mm deep (the distance among defect base and alveolar crest on an “Intraoral Periapical Radiograph” (IOPA) combined with an interproximal residual Probing Pocket Depth (PPD) >5mm were included. Patients diagnosed with aggressive periodontitis, systemic illnesses, insufficient platelet count, pregnant/ lactating mothers, postmenopausal women, and smokers, on a therapeutic regimen were excluded. Intervention After obtaining consent from the participants following clinical parameters were recorded “Plaque Index” (PI)13, and “Modified Sulcus Bleeding Index” (mSBI)14. The PPD and “Clinical Attachment Level” (CAL) were recorded with “University of North Carolina-15” (UNC-15) periodontal probe along with an acrylic stent from marginal gingiva to the depth of the pocket and from “cemento-enamel junction” (CEJ) to the depth of pocket respectively. Radiographic assessment of IBDD was carried out using a computer running Windows XP with ‘Kodak RVG 5000 digital radiography program. Standardized paralleling techniques were used for radiographs. IBDD was 4 Mathew et al. Braz J Oral Sci. 2024;23:e240338 evaluated using Digimizer software. where the resolution of the image was set at a value where each pixel was equal to 0.026mm. The defect depth was computed from the alveolar crest to its base. The readings obtained in pixels were converted to mm by multiplying the pixel with 0.026. The DFDBA’s particle size used was 500μm and obtained from Tata memorial cen- tre, Kharghar, Navi Mumbai. Four weeks following phase I therapy, the selected operative site was anaesthetized with 2% lignocaine hydrochloride using adrena- line (1:80000) and a microsurgical flap for access was planned with microsurgical instruments (Figure 1). Using 3.5×optical magnification dental loupes, buccal & lin- gual sulcular incisions were made using microsurgical ophthalmic blades. Reflec- tion of the mucoperiosteal flap was carried out using the microsurgical periosteal elevator at the test site and control site maintaining as much interproximal soft tissue as possible. (Figure 2, 3a,b,c,d.) Meticulous defect debridement as well as root planning were performed with specific curettes. The preparation for i-PRF was carried out with specifications as described15. Upon termination of centrifugation, the upper orange colour fluid was collected using a 5ml syringe with a 26G needle (Figure 4a,b,c,d). After 5 minutes DFDBA particles were added to i-PRF in a 1:1 ratio and within 15 minutes the material was ready to use with a total working time of 20 minutes. The mixture was delivered to the test site with the help of a bone graft carrier followed by sutures and placement of periodontal dressing. (Figure 5a,b,c,d). The control group received DFDBA granules mixed with saline in the same propor- tion. The mucoperiosteal flaps were stabilized using 5-0 sutures. A periodontal dressing was used to protect the surgical area (Figure 6). Figure 1. Microsurgical instruments with magnifing loupes. 5 Mathew et al. Braz J Oral Sci. 2024;23:e240338 A B C D Figure 2. Preoperative view showing. a) probing depth assessment b) intrabony defect on Digimiser c) placement of the incision d) Flap reflection. A B C D Figure 3. The clinical picture at the control site showing a) probing depth assessment at baseline b) Digimiser showing intrabony defect c) Placement of sulcular incision with microblade d) Flap reflection. 6 Mathew et al. Braz J Oral Sci. 2024;23:e240338 A B C Figure 4. Clinical picture showing i-PRF preparation. a) Centrifuge machine b) Vacutainer c) Obtention of i-PRF d) Loading of i-PRF in syringe. A B C D E Figure 5. Clinical picture showing a) i-PRF delivery into DFDBA b) Mixing of i-PRF and DFDBA c) Grafting at the test site d) Placement of sutures e) Periodontal dressing. 7 Mathew et al. Braz J Oral Sci. 2024;23:e240338 A B C D Figure 6. The clinical picture at the control site showing a) DFDBA b) Grafting c) Placement of sutures d) Periodontal dressing. The sutures were removed after 10 days. A complete re-evaluation of all the clinical and radiographical parameters at the 3rd and 6th months post-surgically was under- taken (Figure 7a,b,c,d). A B C D Figure 7. Postoperative view showing clinical and Digimiser picture at 3 months (a,b) and at 6 months (c,d) at test site showing probing depth assessment and intrabony defect resolution. 8 Mathew et al. Braz J Oral Sci. 2024;23:e240338 ENTROLLMENT ALLOCATION FOLLOW UP ANALYSIS Assessed for eligibility (n = 61) Excluded (n = 31) Did not meet inclusion criteria Randomised (n = 30) Teste group (n = 15) Control group (n = 15) Follow up after 6 months Withdrawals (n = 0) Follow up after 6 months Withdrawals (n = 0) Analysed (n = 15) Analysed (n = 15) Figure 8. CONSORT statement for study description. Randomization Randomization was carried out using computer generated numbers and were allo- cated to test and control group. Statistical method Data measurements were determined at baseline, three, and six months. The para- metric tests were applied to analyse the data statistically. Statistics were accepted as significant at values of p<0.05. Data was provided as descriptive statistics by minimum, maximum range, mean and standard deviation. Intragroup comparison of mean difference of normally distributed variables was analysed using the ANOVA test. Pairwise comparison was done using the Bonferroni Post Hoc test. Intergroup comparison of non-normally distributed variables was done with “Mann Whitney U-test” & an unpaired t-test. The “Statistical Package for the Social Sciences” (SPSS) version 19 was applied to conduct the statistical analysis. Results Sixty-one patients were assessed for eligibility out of which 31 were excluded as they did not meet the inclusion criteria. Thirty sites evaluated in thirty patients out of which 15 sites each were randomly allocated to test and control group. Intragroup comparison within the test and control group presented statistically highly substantial variation of mean site-specific PI, modified SBI, PPD, CAL, and IBDD score during different durations that is from baseline to 3 months & from 3 to 6 months (p<0.001) [Table 1 and 3]. Similar results were obtained with pairwise comparisons within the two groups at (p<0.001) [Table 2, 4]. The inter-group comparison revealed no statistically substantial variation of mean site-specific PI at baseline, 3, and 6 months (p>0.05). The scores of mSBI at 3 9 Mathew et al. Braz J Oral Sci. 2024;23:e240338 months showed statistically significant variation (p<0.05), but not at 6 months (p>0.05). The assessment of PPD and CAL revealed statistically no significant (p>0.05) variation at baseline and six months but in 3rd month, the test group presented a greater reduc- tion in PPD and higher gain in attachment than the control group which was statistically significant (p<0.05). Regarding IBDD, there was statistically no significant variation of mean intra bony depth defect at all 3 intervals between the test & control group (p>0.05) [Table 5]. A change and a comparison of the change in PI, mSBI, PPD, CAL, and IBD from baseline to six months in between the 2 groups was compared with the “Mann-Whitney U test” which revealed that there was statistically no significant (p>0.05) variation of mean changes at six months as depicted. [Table 6 and 7] Table 1. Intragroup comparison of PI, mSBI, CAL, PPD, IBDD score at baseline, 3- and 6-months interval in Test group. PARAMETERS BASELINE 3 MONTHS 6 MONTHS SUM OF SQUARES MEAN SQUARE F P VALUE PI 2.80±0.41 1.73±.458 0.87±.352 28.133 14.067 75.74 <0.001 mSBI 2.73±.458 1.13±.352 0.67±.488 32.044 16.022 60.807 <0.001 PPD 8.00±2.138 4.40±1.056 1.87±1.407 284.978 142.489 55.757 <0.001 CAL 6.27±1.831 2.80±1.320 0.47±.516 255.511 127.756 71.480 <0.001 IBDD 5.07±0.80 3.20±.86 1.67±.61 86.978 43.489 117.5889 <0.001 IBDD-Intrabony defect depth, “CAL-Clinical attachment level, PPD-Probing Pocket Depth”, mSBI-modified Sulcus Bleeding Index, F-ANOVA Test, PI-Plaque Index, P-Probability value. (p<0.05) Table 2. Pairwise difference of PI, mSBI, CAL, PPD, and IBDD at baseline, 3 to 6 months interval in the Test group. PARAMETERS MEAN DIFFERENCE BASELINE-3 MONTH BASELINE-6 MONTH 3-6 MONTHS PI 1.07 1.93 .867 mSBI 1.600* 2.067* .467* PPD 3.600* 6.133* 2.533* CAL 3.467* 5.800* 2.333* IBDD 1.87 3.40 1.53 PPD-Probing Pocket Depth, mSBI- “modified Sulcus Bleeding Index, CAL-Clinical attachment level,” PI-Plaque Index, IBDD-Intrabony defect depth. (p<0.05) Table 3. Intragroup comparison of PI, mSBI, CAL, PPD, IBDD score at baseline, 3 months 6 months in the test group. PARAMETERS BASELINE 3 MONTHS 6 MONTHS SUM OF SQUARES MEAN SQUARE F P VALUE PI 2.60±0.51 1.60±.507 .60±.507 30.000 15.000 58.333 <0.001 mSBI 2.47±.516 1.47±.516 .40±.507 32.044 16.022 60.807 <0.001 PPD 7.00±1.648 3.67±.724 1.27±1.100 248.711 124.356 83.880 <0.001 CAL 5.07±1.438 1.80±.676 .27±.458 180.311 90.156 98.951 <0.001 IBDD 4.53±.640 2.93±.704 1.27±.704 80.044 40.022 85.762 <0.001 PPD-Probing Pocket Depth, mSBI- “modified Sulcus Bleeding Index, CAL-Clinical attachment level”, PI-Plaque Index, IBDD-Intrabony defect depth. (p<0.05). 10 Mathew et al. Braz J Oral Sci. 2024;23:e240338 Table 4. Pairwise difference of PI, mSBI, CAL, PPD, and IBDD in between baseline, 3 & 6months intervals in the control group. PARAMETERS MEAN DIFFERENCE “BASELINE-3 MONTH BASELINE-6 MONTH 3 MONTH-6 MONTHS” PI 1.000* 2.000* 1.000* mSBI 1.000* 2.067* 1.067* PPD 3.333* 5.733* 2.400* CAL 3.267* 4.800* 1.533* IBDD 1.600* 3.267* 1.667* PPD-Probing Pocket Depth, m SBI-modified Sulcus Bleeding Index, CAL-Clinical attachment level, PI-Plaque Index, IBDD-Intrabony defect depth. (p<0.05). Table 5. Intergroup comparison of PI, mSBI, CAL, PPD, and IBDD at baseline, 3 & 6 months. PARAMETERS BASELINE 3 MONTHS 6 MONTHS TEST GROUP CONTROL GROUP TEST GROUP CONTROL GROUP TEST GROUP CONTROL GROUP” PI 2.80±0.41 2.60±0.51 1.73±.458 1.60±.507 .87±.352 .60±.507 mSBI 2.73±.458 2.47±.516 1.13±.352 1.47±.516 .67±.488 .40±.507 PPD 8.00±2.138 7.00±1.648 4.40±1.056 3.67±.724 1.87±1.407 1.27±1.100 CAL 6.27±1.831 5.07±1.438 2.80±1.320 1.80±.676 .47±.516 .27±.458 IBDD 5.07±0.80 4.53±.640 3.20±.862 2.93±.704 1.67±.617 1.27±.704 PPD-Probing Pocket Depth, m SBI-modified Sulcus Bleeding Index, CAL-Clinical attachment level, PI-Plaque Index, IBDD-Intrabony defect depth. (p<0.05). Table 6. Change in Pi, SBI, PPD, and IBD from baseline to 6 months between the 2 groups. By 6 months Change in Group Minimum Maximum Mean Std. Deviation PI Test 1 3 1.93 .060 Control 1 3 2.00 .37 SBI Test 1 3 2.07 .46 Control 1 3 2.07 .45 PPD Test 2 10 6.13 2.4 Control 3 9 5.73 1.8 CAL Test 3 9 5.80 1.82 Control 2 8 4.80 1.56 IBD Test 2 6 3.40 0.91 Control 3 4 3.27 .45 m SBI-modified Sulcus Bleeding Index, PPD-Probing Pocket Depth, CAL-Clinical attachment level, PI-Plaque Index, IBDD-Intrabony defect depth. (p<0.05). 11 Mathew et al. Braz J Oral Sci. 2024;23:e240338 Table 7. Comparison of change in PI, mSBI, PPD, and IBD from baseline to six months between the 2 groups. Six months PI change Six months mSBI change Six months of PPD change Six months CAL change Six months IBDD change Mann-Whitney U 105.500 112.500 104.000 82.500 104.50 Z-score -.448 .000 -.360 -1.271 -0.379 P value .654 1.000 .719 .204 .704 PPD-Probing Pocket Depth, m SBI-modified Sulcus Bleeding Index, CAL-Clinical attachment level, PI-Plaque Index, IBDD-Intrabony defect depth. Z-score. (p<0.05). Discussion The primary purpose of periodontal therapy is resolution of inflammation, elimination of infection, disease progression cessation, and prevention of recurrence16. DFDBA was chosen in this study due to its inductive properties of stimulating host stem cells to differentiate into osteoblasts. Its regenerative properties have stimulated physi- cians to broadly utilize it in infrabony defects17. In addition DFDBA has been shown to withstand displacement due to its physical property2. Platelet concentrates possess growth factors that act as vital modulators induc- ing the differentiation, proliferation, attachment, and migration of periodontal pro- genitor cells5. Advantages of using platelet concentrates is its simpler preparation, inexpensive nature and antibacterial effect due to presence of leukocytes18. With the speculation, that the addition of autologous plasma derivatives like PRP, PRF, and i-PRF might show synergistic and predictable outcomes, this study combined these two biomaterials that is i-PRF and DFDBA. i-PRF can be employed to mix graft cohesively and sprayed over surgical sites due to its injectable form19. i-PRF remark- ably influences osteoblast behaviour by impacting the migration, proliferation, and differentiation of human osteoblasts according to a study and has been shown to complement graft materials in aiding regeneration20,21. Taking into consideration the above aspects, the efficiency of i-PRF with DFDBA and DFDBA alone was tested clin- ically and radiographically. Assessment period of 6 months for evaluation of radio- graphs was chosen as evidence of rising bone density is frequently not observed until then1. The clinical parameters studied were PI, mSBI, PPD, CAL and IBDD. PI showed statis- tically high significant difference between the three intervals in both groups depict- ing good plaque control which could be due to reinforcement of oral hygiene instruc- tions. However intergroup comparison revealed no statistically substantial difference. This was in line with research conducted by Sharma and Pradeep22, Elgendy and Abp Shady20 and Agarwal et al.5. Modified SBI was recorded to assess the presence or absence of gingival inflamma- tion. Both the groups showed a remarkable reduction in inflammation at various inter- vals. The outstanding potential of i-PRF being an anti-inflammatory has been high- lighted due to its property of reducing proinflammatory M1 macrophage phenotype and activation of dendritic cells23. 12 Mathew et al. Braz J Oral Sci. 2024;23:e240338 PPD was used to evaluate the effectiveness of the therapy and a gain in the CAL remains the gold standard for evaluating the success of periodontal treatment. A reduction in PPD from baseline to six months was noted in the test and control group which was statistically highly significant. At 3 months, the test group fared better than the “control group” [p<0.05], but intergroup comparison however failed to reach a statistically significant difference at 6 months suggesting that i-PRF combined with DFDBA as well as DFDBA alone when used was effective in reducing the probing pocket depth. Progressive improvement in the mean CAL from baseline to three months and from 3-6 months was observed which was highly significant in both groups. However inter- group comparison revealed no statistical significance. These outcomes were consis- tent with the research performed by Khosropanah et al.24 and Agarwal et al.5. Gain in CAL is attributed to raised resistance to probing caused by a drop in inflammation, gingival fibers reformation, and a long junctional epithelium25. In the current work, the mean IBDD in both groups from baseline to six months was statistically highly significant (p-value <0.05). Both the groups demonstrated a drop in defect depth with formation of new bone on the radiograph. Radiographically better bone fill was observed in the test group. This might be attributed to the addition of i-PRF with DFDBA. However, the test group failed to reach a statistically significant value. The outcomes are in line with the research performed by Agrawal et al.5 and Khosropanah et al24. Refinements in surgical procedures have entailed use of the minimally invasive surgi- cal method which create minimum flap reflection, minimal wounds, and gentle han- dling of the hard & soft tissues26. The benefits include better visual acuity, ease of soft tissue handling, and precise wound closure, enabling healing with primary inten- tion27. In the present study, microsurgical flap access was employed that allowed a more accurate, less traumatic access to periodontal defect however no parameters were included to evaluate the impact of the microsurgical approach on the healing of the flap. Histologic analysis or surgical re-entry was not opted due to ethical reasons which were limitations of the study. Conclusion In the current research, it may be inferred that DFDBA alone or in combination with i-PRF can be used to treat 3-walled intrabony defects. Thus, the future scope of the study includes exploring i-PRF’s potential to be used for its anti-inflammatory property in conjunction with other bone graft materials for which more longitudinal studies are required. Funding None. Conflicts of interest None. 13 Mathew et al. Braz J Oral Sci. 2024;23:e240338 Authors’ Contribution All authors actively participated in the discussion of the manuscript’s findings and have revised and approved the final version of the manuscript. 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