1 Volume 22 2023 e231269 Original Article Braz J Oral Sci. 2023;22:e231269http://dx.doi.org/10.20396/bjos.v22i00.8671269 1 Department of Materials Science, Military Engineering Institute, RJ, Brazil. 2 Department of Prosthesis and Dental Materials, Dental School, Federal University of Rio de Janeiro, RJ, Brazil. Corresponding author: Marvin do Nascimento Rua Almirante Tamandare, 66 Flamengo, Rio de Janeiro, RJ, Brazil. Tel: +55 21 96642-8431 E-mail: mvnascimento@hotmail. com.br Editor: Dr. Altair A. Del Bel Cury Received: October 19, 2022 Accepted: November 08, 2022 The peri-implant ligament: a scoping review Marvin do Nascimento1,* , Bruno Martins de Souza1 , Aline Tany Posch2 The peri-implant ligament is formed from the interface of bone tissue, through the anchoring of proteins and the surface of the dental implant. In this sense, it is relevant to understand the extent to which this ligament is structured and biomimics the periodontal ligament functions. Aim: The goal of this scoping review is to present and analyze the peri-implant ligament composition and compare the extent to which this ligament is structured and biomimics the periodontal ligament functions. Methods: This scoping review was performed according to the Joanna Briggs Institute methodology for scoping reviews and following the Preferred Reporting Items for Systematic Reviews and Meta-analyses extension for scoping review. Two independent researchers searched Pubmed, Cochrane, Embase, Virtual Health Library, Scielo, Scopus, Web of Science, Brazilian Bibliography of Dentistry, Latin American and Caribbean Literature in Health Sciences, Digital Library of Theses and Dissertations from the University of São Paulo and Portal Capes. Studies published in English, Portuguese and Spanish, over the last 21 years (2000-2021). Results: A total of 330 titles were identified and after applying inclusion and exclusion factors, 27 studies were included in this review. All proteins were identified regarding their tissue function and classified into 6 major protein groups. After that this new protein ligament was compared with the periodontal ligament regarding its function and composition. The main proteins associated with osseointegration, and thus, with the peri-implant ligament are recognized as belonging to the periodontal ligament. Conclusion: This scoping review results suggest evidence of the composition and function of the peri- implant ligament. However, variations may still exist due to the existence of several modulants of the osseointegration process. Keywords: Osseointegration. Dental implants. Biocompatible materials. Proteins. https://orcid.org/0000-0001-8010-7382 https://orcid.org/0000-0002-1075-0441 https://orcid.org/0000-0002-4501-4161 2 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 Background The peri-implant ligament can be described as an osteoconductive protein- aceous ligament that will support the osteoconduction space during the osse- ointegration process. This ligament will mediate the interactions between bone tissue cells, and thus, osteoblasts with the implanted biomaterial, and more spe- cifically, a relationship between the alveolar periosteum and the osseointegrable dental implant1,2. However, it is noteworthy that the osseointegration process will only occur from a periodontal health status and excellent biocompatibility. Generally, this biocompat- ibility has been achieved from titanium implants and their alloys, such as titanium commercially pure grade IV, titanium-aluminum-vanadium, titanium-zirconia and even zirconia implants have proven to be a successful replacement1-3. These osseointegrable implants will allow osteoinduction and provide the process of bone formation and remodeling from the protein’s adsorption on the topographic implant surface. Thus, there is no direct contact between the bone tissue and the dental implant, but an interface mediated by the anchoring of cellular proteins and the implants’ surface4. To the best of our knowledge, there is still no consensus as to the proteins partic- ipating in this bone-integration process to the point of constituting a peri-implant ligament. Therefore, the purpose of this scoping review is to present and analyze the peri-implant ligament composition and compare the extent to which this ligament is structured and biomimics the periodontal ligament functions. Materials and Methods This scoping review was performed following the Joanna Briggs Institute methodol- ogy for scoping reviews5 and Preferred Reporting Items for Systematic Reviews and Meta-analyses extension for Scoping Review (PRISMA-ScR)6 flow diagram. Further- more, the protocol for this review was registered and submitted to the Open Science Framework (OSF) platform (DOI 10.17605/OSF.IO/9SD5H) and the statistical results were done with Microsoft Excel and Orange 3.30.1 Review Question • To what extent can the peri-implant ligament mimic the composition and func- tions of the periodontal ligament? Selection of Studies The PCC method was used (Table 1). Studies that met the following inclusion criteria were included in this review: osseointegrable dental implants; protein/protein adsorp- tion within the osseointegration process. The study exclusion criteria were as follows: address some pathology; systemic health changes; smoking; grafts; bruxism; ortho- dontics; periodontitis. 3 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 Table 1. Election Criteria with the PCC Method Participants Concept Context Osseointegrable implants and Dental implants. Exclusion criteria: non-dental and non-osseointegrable implants. Peri-implant ligament and protein adsorption on the implant surface. Biocompatibility (not foreign body response) and periodontal health (not periodontal disease). Search Strategy and Data Extraction The search was performed on databases: Pubmed (MEDLINE), Cochrane CENTRAL, Embase, Virtual Health Library (VHL), Scielo, Scopus and Web of Science (WoS), and in gray literature: Brazilian Bibliography of Dentistry (BBD), Latin American and Carib- bean Literature in Health Sciences (LILACS), Digital Library of Theses and Disserta- tions from the University of São Paulo (USP Theses) and Portal Capes. The search strategy was performed by two independent researchers and adapted for each data- base (Table 2). Thus, studies in English, Portuguese and Spanish will be included due to the potential for international access and also those published since the last 21 years (2000-2021) as a consideration of new concepts in modern literature. Therefore, after searching the literature, duplicate articles were removed. Then, the titles and abstracts were selected by two independent reviewers for evaluation against the inclusion criteria for the review. The full text of selected citations was evaluated in detail against inclusion criteria by two independent reviewers. Reasons for excluding sources of evidence in the full text that do not meet the inclusion criteria will be recorded and reported in the scoping review. Any disagreements that arose between reviewers at each stage of the selection process were resolved through dis- cussion or with an additional reviewer. Table 2. Methodology and Search Key by Digital Platform Data Base Search Key Inclusion Criteria Exclusion Criteria Pubmed (((“biomaterials”[All Fields]) AND (“dental implants”[All Fields])) AND (“osseointegration”[All Fields])) AND (“proteins”[All Fields]) Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) non-dental implants; non-osseointegrated implants; cancer Cochrane “osseointegration” AND “dental implants” AND “proteins” Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) articles not available; non-dental implants; articles that do not address protein adsorption Embase osseointegration AND ‘osseointegrated implant’ AND protein Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) non-dental implants; non-osseointegrated implants; cancer VHL (osseointegração) AND (implantes dentários) AND (adsorção de proteínas) AND (year_cluster:[2000 TO 2021]) Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) articles that do not address protein adsorption Scielo (osseointegração) AND (protein) Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) did not answer the question Continue 4 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 Continuation Scopus (TITLE-ABS-KEY ( osseointegration ) AND TITLE-ABS-KEY (dental AND implants ) AND TITLE-ABS-KEY ( protein ) AND TITLE-ABS-KEY ( adsorption AND of AND proteins ) ) Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) non-dental implants; non-osseointegrated implants; WoS TÓPICO: (osseointegration) AND TÓPICO: (biomaterials) AND TÓPICO: (dental implants) AND TÓPICO: (protein) AND TÓPICO: (adsorption of proteis) Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) non-dental implants; non-osseointegrated implants; BBD (osseointegração) AND (adsorção de proteínas) AND (year_cluster:[2000 TO 2021]) Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) articles not available LILACS osseointegração AND proteínas AND ( db:(“LILACS”)) AND (year_cluster:[2000 TO 2021]) Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) articles not available; articles that do not address protein adsorption USP osseointegração AND implantes AND adsorção de proteínas Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) non-dental implants; non-osseointegrated implants; Portal Capes osseointegration AND protein AND (adsorption of proteins) AND dental implants Full texts, Portuguese, English and Spanish, in the last 21 years (2000-2021) non-dental implants; non-osseointegrated implants; articles that do not address protein adsorption; duplicate articles Statistical Analysis All protein-related parameters were considered as results for the distribution of pro- teins into classes. Average and standard deviation (SD) values were estimated from sample size, average mean, average’s distance, average’s square and variance. The average sum among (between), within (in), and total was also used. In the results, 95% confidence level (CI) was considered. The chi-square test was performed to associate the data distribution with distribution relationship and protein association. The significance level for chi-square was set at 0.05. To evaluate the useful hypoth- eses comparing this distribution the T-test was performed. The significance level for the T-test was set to 0.05. Results A total of 330 articles were identified during the search. After screening with the reading of the title and abstract, 156 articles remained, then 48 articles were selected to be read in its entirety, and after applying the inclusion and exclusion criteria, in addition to the exclusion of repeated articles, 27 articles were selected. Among the excluded articles, the main reasons were papers that did not answer the question, dropped the subject or even addressed it, but the content did not approach the issue of interest. The selected articles were based, mainly, on proteins that appeared in 5 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 osseointegration, either through research of tests with implants, or that addressed this information in the body of the article (Figure 1). Identification of studies via databases and registers Id en tifi ca tio n Sc re en in g In cl ud ed Studies identified in databases (n = 330) Studies selected by electronic search extraction (n = 156) Studies selected for full-text reading and data extraction (n = 48) Studies not selected (n = 174) Studies duplicate (n = 21) Total studies included (n = 27) Studies excluded (n = 73) and duplicate (n = 35) total (n = 108) Figure 1. Study Selection Flowchart The studies included in this scoping review are summarized in Table 3. Most of the results did not specify which implant they were based on. The implants described were: commercially pure grade IV titanium (Ticp), titanium-aluminum-vanadium (Ti-6Al-4V), titanium-zirconia (Ti-ZiO2), titanium-zirconia-tantalum (Ti-ZiO2-Ta), titanium-zirconia-tantalum-nitrogen-sulfur (Ti-ZiO2-Ta-N-S), Ti-machined and Ti-sandblasted and etched. These studies provide data for a total of 87 proteins (sum total of 167 proteins) that were classified into seven protein classes: cell membrane, complement system, cytoskeleton, extracellular matrix, factors, plasma, saliva (Figure 2). 6 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 Figure 2. Osseointegration Proteins Statistical Distribution. Distribution of proteins in the seven protein classes (%), chi-square test, p < 0.05. Table 3. Relation of Proteins in the 27 studies Reference (Author and Year) Implants Considered Related Proteins in the Osseointegration Process Barberi and Spriano, 20217 Ticp; Ti-6Al-4V; Integrin (α5β1); Fibronectin; Vitronectin; Bone Morphogenic Protein-2; Fibrinogen; Albumin; Laminin α; Lactoferrin; Collagen type I; Salivary Proteins; Meyer et al., 20048 not specified Fibronectin; Vitronectin; Osteonectin; Romero-Gavilán et al., 20179 Ti machined; Ti sandblasted and acid etching; Integrin αV; Vitronectin; Beta-2-Glycoprotein 1; Complement System (C3; C4α; C6; C8β); CNNM2 metal conveyor; Junction Plakoglobin; Serum Paraoxonase/Arylesterase 1; Plasminogen; Coagulation Factor XII; Gelsolin; Alpha-2-Macroglobulin; Tetranectin; Pigment Epithelium-Derived Factor; Alpha-1-acid glycoprotein (1; 2) Apolipoproteins (ApoA1; ApoC1; ApoE); Proteoglycan 4; Peptidyl-prolyl Cis-Trans Isomerase β; Haptoglobin-Related Protein; Vitamin D Binding Protein; Cytoplasmic Actin 1; Lysozyme C; Hemoglobin β Subunit; Kininogen-1; Serotransferrin; Ceruloplasmin; Plectin; Antithrombin III; Vitamin K Dependent Protein; Continue 7 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 Continuation Martínez-Ibáñez et al., 201810 Ti machined; Fibronectin; Fibrinogen; Albumin; Collagen type I; Prati et al., 201311 not specified Osteoprotegerin; Osteocalcin; Osteopontin; Parathyroid Hormone; Transforming Growth Factor α; Cei et al., 201512 not specified Integrin; Fibronectin; Vitronectin; Albumin; Collagen type I; Matsumoto et al., 202013 not specified Fibronectin; Albumin; Parisi et al., 202014 not specified Fibronectin; Albumin; Wu et al., 202015 Ti-ZiO2; Ti-ZiO2-Ta; Ti-ZiO2-Ta-NS; Albumin; Osteocalcin; Osterix; Osteoprotegerin; OSX; OPG; Receptor Activator of Nuclear Factor Kappa B Ligand; Receptor Activator of Nuclear Factor Kappa B; Parisi et al., 201916 not specified Fibronectin; Vitronectin; Vinculin; Dayan et al., 201917 not specified Integrin; Subramani et al., 201918 not specified Integrin; Fibronectin; Vitronectin; Focal Adhesions; Boyan et al., 201719 Ticp; Ti-6Al-4V; Ti-ZiO2; Integrin (α5β1; α2β1; α1β2; α1; α2; α5; αv; β1; β3); Fibronectin; Collagen type 1; Osteoprotegerin; Receptor Activator of Nuclear Factor Kappa B Ligand; Transforming Growth Factor β (1; 2; 4; 7); Cho et al., 201620 not specified Integrin (α5β1); Fibronectin type III; Chen et al., 201421 not specified Integrin; Fibronectin; Fibrinogen; Laminin; Bone Morphogenic Protein-2; Osteogenic Growth (OGP); Lee and Ogawa, 201222 not specified Fibronectin; Albumin Hori et al., 201023 not specified Integrin; Fibronectin; Albumin; Lavenus et al., 201024 not specified Integrin; Fibronectin; Vitronectin; Albumin; Focal Adhesions; Hori et al., 200925 not specified Fibronectin; Collagen; Albumin; Rupp et al., 201826 not specified Fibronectin; Vitronectin; Broggini et al., 201227 not specified Integrin; Fibronectin; Vitronectin; Collagen; Laminin; Fibrin; Bone Sialoprotein; Osteopontin; Thrombospondin; Jaiswal el al., 201928 not specified Albumin; Fibrinogen; Globulins; Bone Sialoprotein; Osteocalcin; Osteonectin; Kopf et al., 201529 Ticp; Ti-ZiO2; Fibronectin; Fibrinogen; Rapuano et al., 201230 Ticp; Ti-6Al-4V; Fibronectin; Bone Sialoprotein; Integrin α5β1; Petrie et al., 200931 not specified Integrin (α5β1; αvβ3; α3β1; α8β1; α2β1); Fibronectin; Laminin; Collagen; Bone Sialoprotein; Osteopontin; Salvoni, 200632 not specified Integrin; Wei et al., 202033 Ti;ZiO2 Fibronectin; Vitronectin; Albumin; Laminin γ; Fibrinogen; Glycosaminoglycans; Collagenase; Apolipoprotein; Complement System Proteins; Phosphopeptides; Haptoglobin; Hemopexin; Salivary Cystatin; Histatin; Statherin; In the cell membrane, proteins showed the highest percentage rate, 83,91%, being the most prevalent α5β1 (16,13%), α2β1 (6,45%), αV subunit (6,45%) and an integrin groups could not be classified at the subunit level (25,81%). In the extracellular matrix had a higher abundance of fibronectins (31,82%), being that of fibronectins type III (1,52%), vitronectin (15,15%), collagenous fibers (10,61%), which 6,06% represented type I, 8 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 laminin (7,59%), these 1,52% represented the α and γ subunits (each), osteoprotegerin (6,06%), and bone sialoprotein-2 (6,06%). In the factors group, the most abundant was fibrinogen (35,29%) followed by transforming growth factor (29,4%), with 5,88% for factors α, β1, β2, β4, β7 (each). In plasma albumin was the most abundant protein (31,58%) followed by apolipoprotein (13,15%), with the C1 subunit being 2,63% and 5,26% the A1 and E subunits (each). Protein Data All the protein groups will participate at different times in the osseointegration pro- cess. In the first moment after implant installation, there will occur the protein adsorp- tion of blood plasma and factors (mediating the angiogenesis process), complement system and almost at the same time salivary proteins. After osteoconduction, the recognition and interaction between osteoblasts and implant will be mediated by cell membrane proteins, cytoskeleton, and bone formation and remodeling by the extra- cellular matrix bone proteins. Figure 3 shows the proteins network distribution in the osseointegrated process. Figure 3. Multidimensional Protein Scaling. Multidimensional scaling projecting the proteins on adjusted plane representing the distances between points. t-test, p < 0.05. Discussion The present study explored the proteins involved during the osseointegration pro- cess of osseointegrable implants. Several proteins have been previously reported with greater frequency, but the idealization of periodontal ligament regeneration has always been denied. Therefore, this study sought to think about how each protein and protein grouping would behave during the integration process. Con- 9 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 sidering the existence of a peri-implant ligament, we decided to investigate to what extent this ligament biomimics the functions of the periodontal ligament. Thus, we hypothetically considered osseointegration compatible with the state of periodontal health. Osseointegrable implants cannot be in direct contact with bone tissue, since this would not fulfill the tertiary stability function, which is related to the dissipation of these loads after osseointegration, which would result in bone fracture. Thus, the idea that there is a protein ligament around the implant becomes more accepted from this perspective. Thus, based on agreements and disagreements with the studies in this review, we reached a hypothesis and can make the following consideration: the two ligaments have practically the same composition. Although the periodontal ligament is described predominantly with Sharpey’s fibers that are permeated by collagenous fibers type III, this type of collagenous fiber is not often found alone because it is more fragile (thin), so it is a supportive col- lagenous fiber that in most cases is accompanied by collagenous fibers type I. In addition, the periodontal ligament contains several collagenous fibers types, such as type I, III, V, VI, XII34. The periodontal ligament cellular components are 50-60% fibroblasts, immune system defense cells, osteoblasts and osteoclasts (hard blade residents), cemen- toblasts, stem and progenitor cells. Protein components of the extracellular bone matrix such as collagen fibers type I, BMP-2, TFG-β, osteocalcin, osteopontin, osteonectin, bone sialoprotein, fibronectin, and proteoglycans are also present35. The periodontal ligament in anatomical classification is described as a gonphous joint and, although it is described as a joint, there are no significant levels of carti- laginous tissue in this region, even though our research found chondroitin sulfate proteoglycan 4 (CSPG4) which is produced by chondroblasts and has the function of lubricating the medium36. Furthermore, the periodontal ligament itself does not have the same nourishing function as the tooth, since the periodontal space is composed of an unformed dense connective tissue with the presence of blood vessels and nerve plexuses. Thus, the tooth is nourished through diffusion determined by the concentration of gradient driving force. This ligament has both sensory and support functions. The first is related to the perception of the tooth in the body, and the second to the sup- port and dissipation of masticatory loads. And these two functions are compatible with the functions of the peri-implant ligament. In this sense, starting from osteoblasts we will divide the interpretations on the role of protein components mediating the connections with the integrin (inside-out binding), in such a way that biochemical mechanisms are configured to promote affinity alter- ations for specific ligands. Generally, these mediations will occur by binding to actin filaments through talin (adaptor protein) or intermediate filaments37. Thus, starting from the osteoblast cell membrane integrins, the only possibilities for binding of these proteins end up in: 10 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 The integrins α1β2, α2β1 are collagen receptors; the α2β1, α3β1, α5β1, α8β1, αVβ1 are recep- tors for fibronectin; the α5β1 fibrinogen receptor; the α2β1 are receptors for laminin; the α3β1 is a receptor for epiligrin, thrombospondin and CSPG4. Some α1, α2, α5, αV, β1, β3 subunits were not specified in the studies analysed. However, The α1 subunit can only bind with β1 or β2, and the α2 can only bind with β1, which in any case will characterize as collagen fiber receptor; the α5 subunit can only and bind with β1, which characterizes it as fibronectin receptor; the β1 subunit recognizes the RGD sequence on a wide range of ligands of α1 (collagen fibers and laminin), α2 (collagen fibers and laminin), α3 (fibronectin, collagen fibers and laminin), α4 (fibronec- tin and VCAM-1), α5 (fibronectin), α6 (laminin), α7 (laminin), α8 (osteopontin, vitronec- tin, fibronectin and nephronectin), α9 (tenascin-C, VEGF-C and VEGF-D), α10 (collagen fibers), α11 (collagen fibers) and αV (fibronectin, vitronectin and osteopontin); the β3 sub- unit can only and make bonds with αIIb (fibronectin, fibrinogen, von Willebrand Factor, thrombospondin, and vitronectin) or with αV (osteopondin and collagenous fibers). Thus, in the extracellular space the binding possibilities will be mediated with plasma proteins, complement system globulins and with the factors while the angiogenesis process occurs (initial moment). The salivary proteins will not have direct contact of specific binding with the membrane proteins, they will only subsidize the acquired film formation. As for the constituents of the extracellular matrix, we can highlight some bone matrix proteins such as BMP-2, IBSP, SPARC, OPG, BGLAP, SPP1, COL-1 and GAG that will participate in bone formation and remodeling. Thus, the possible and main structures between plastic membrane and implant, based on integrin bind- ing possibilities, are: laminin, fibronectin, vitronectin and collagen fibers. Laminin is a plasma membrane protein that constitutes the basal membrane. It is a receptor for integrin collagen fibers type IV, heparan sulfate (GAG) has as their main function the organization of the extracellular matrix. The fibronectin binds to cell surfaces by means of the integrin and is able to bind various compounds, including collagen fibers, fibrin, heparin, DNA and actin. It par- ticipates in the process of adhesion, cell locomotion, opsonization, regeneration, and maintenance of cell shape. It acts in the matrix assembly and thus in fibrillogenesis (essential for osteoblast mineralization), besides participating in the regulation of col- lagen fibers type I deposition (matrix secretion). Vitronectin is an integrin receptor protein and can interact with GAG’s and proteo- glycans. It participates in cell adhesion and dissemination, literally functions as a cell-substrate adhesion molecule. Collagen fibers type I (with portions of type III and V) are the main constituent of the extracellular matrix. They can bind with integrin, fibronectin, vitronectin. Specifically, collagen type IV (non-fibrillar) has an affinity for laminin, for collagens XV and XVIII, and proteoglycans. Collagen fibers type VI associate with type I and III to form microfibrils and are stabi- lized by non-covalent GAG bonds; collagen fibers type XII also associate with colla- gen fibers type I; collagen type XV is a constituent of the basal membrane; collagen type XVII participates in the adhesion process of epithelial cells with the extracellular matrix and is a receptor for laminins and integrins. 11 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 Thus, our hypothesis is based on a structure similar to the basal membrane. When thinking about the concept in a broad way, the osteoconduction space will perform the role of basal membrane, since it has the same characterizations, the same constituents, and basically the same function. However, the concept of the basal membrane does not compete with the tissues involved, this being a characteristic, with high specificity of epithelial tissue (ectoderm), so that the basal membrane serves to join the epithelial tissue to the adjacent connective tissue. So, in this case, specifically, there is the bone tissue (mesoderm) and the implant, and both do not have any epithelial characteristic, because there is no epithelium, but there is specialized connective tissue (bone tissue). Therefore, it can be considered a structure similar and compatible with the basal mem- brane, but that is not characterized as the basal membrane, properly speaking. Thus, by convention we decided to call it the peri-implantar membrane, characterizing it as a delegate of the basal membrane, which has the delegated competence, but does not hold the position of basal membrane (Figure 4). A B Figure 4. Interaction of Peri-implantar Membrane with the Implant Surface 12 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 Despite these perspectives, there is research exploring mechanisms of periodon- tal ligament regeneration in osseointegrable implants either through biomimetic routes, implant surface treatments, or even enzymatic routes. However, this study suggests that the ligament regenerates, or rather that there is an attempt to regenerate them. Thus, due to virtually the same composition, it can be suggested that the periodontal ligament attempts to regenerate, however, due to some differences in specifications regarding constituents, and for not being able to provide the “nourishing function”, there is a differentiation between both. Thus, we can consider that the peri-implant ligament can biomimicry the functions and composition of the periodontal ligament or consider that the peri-implant ligament is a consequence of the regeneration of the periodontal ligament. In addition, the osseointegration concept has its complexity, especially in post-Brånemark concepts. Thus, understanding the role of these proteins during the osseointegration process is not something easy and simple. However, it is pos- sible to distinguish that the proteins that participate in osteoblast adhesion are those that are part of the cell membrane and the proteins of the temporary matrix that are formed immediately after implant insertion. Furthermore, it is suggestive that a ligamentous structure similar to the periodontal ligament is formed, since there is similarity in composition and function between both ligaments. After all, if there were no ligament between the implant and bone, there would be no distribution of masticatory loads and therefore maxillary/mandibular fracture would occur. Thus, the peri-implant ligament is a consequence of the osse- ointegration process. In that manner, as a limitation of this study we can mention the absence of protein distinction in implants with different surface treatments because most of the results did not make this very clear. This limited a possible statistic about it. However, the role of surface topography in implants is already known. There is already an identification of roughness and wettability parameters as well as surface treat- ments that directly influence the osseointegration process itself. Furthermore, this study only considered the osseointegration process in the setting of systemic health and periodontal health. It disregards patients with different systemic health and periodontal conditions. In conclusion, this systematic scoping review points to evidence suggesting the existence of a peri-implant ligament. This ligament has a composition similar to the periodontal ligament and can mimic sensory and support functions. However, future studies on molecular analysis regarding the composition of the peri-implant ligament and characterizations of this ligament are needed for a better understanding. This is in addition to longevity analyses regarding the success of osseointegrable implants, differences in ligament composition in relation to implant composition, and differ- ences and changes in different periodontal profiles. Furthermore, the variants that can modulate the osseointegration process will probably also intervene in the forma- tion process of this peri-implant membrane. 13 Nascimento et al. Braz J Oral Sci. 2023;22:e231269 Funding No funding sources. Conflicts of interest There is no conflict of interest in this project.  Data availability statement Datasets related to this article can be found at https://osf.io/9sd5h/, hosted at Open Science Framework (OSF). 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