Bull 145 Hayder Badry Ali & Ruia Safwan Kamal Bull. Iraq nat. Hist. Mus. December, (2018) 15 (2): 145-151 FAUNISTIC REVIEW OF THE GENUS CHAITOPHORUS KOCH, 1854 (APHIDIDAE, CHAITOPHORINAE, CHAITOPHORINI) WITH NEW RECORD SPECIES FOR IRAQ APHID FAUNA Hayder Badry Ali* and Ruia Safwan Kamal Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq * Corresponding author e-mail: hayder.ali1130@yahoo.com Received Date: 12 May 2018 Accepted Date: 26 June 2018 ABSTRACT A faunistic review of the genus Chaitophorus Koch, 1854, including four species in Iraq is given; the distribution data of each species and their hosts have been recorded. In this investigation the poplar leaf aphid Ch. populialbae (Boyer de Fonscolombe, 1841) is recorded here for the first time in Iraq on popular trees Populus euphratica Oliv. during the period from November 2016 to April 2017 in Baghdad province. A brief description for apterous viviparous female of this species is given; and a key to the species of the genus Chaitophorus is constricted. Key words: Aphid fauna, Aphididae, Chaitophorinae, Chaitophorus, Iraq. INTRODUCTION The subfamily Chaitophorinae (Homoptera: Aphididae) divided into two tribes Chaitophorini and Siphini, comprises about 170 species within 12 genera (Wieczorek et al., 2017); the distribution range of this subfamily is mainly in the Palaearctic (about 80% of species), and Nearctic region (Wieczorek, 2010; Zhu et al., 2017). Most of the species of the tribe Chaitophorini are monoecious holocyclic and infest plant families which belong to Salicaceae (Populus and Salix) and Aceraceae (Blackman and Eastop, 1994), there is a difficulty to distinguish between species of Chaitophorinae based on their morphological characteristics, although the tribe Chaitophorini has clear morphological differences between genera; separating between Chaitophorus species using morphology has always been problematic because of the fact that the morphological differences between species within these genera are relatively slight (Hille Ris Lambers, 1960; Pintera, 1987; Zhu et al., 2017). Chaitophorus is a typical genus of the Chaitophorinae: it is the largest genus in the subfamily and is well known from Europe and Asia (Pintera, 1987); This genus consists of about 109 valid species (Zhu et al., 2017) and distribution in the Palaearctic and Nearctic zoogeographical regions; in addition to the species of plant family Salicaceae as a host some Chaitophorus species infest limited host belongs to Vitaceae, Daucaceae and Compositae (Pintera, 1987). http://dx.doi.org/10.26842/binhm.7.2018.15.2.0145 146 Faunistic review of the genus Chaitophorus Koch Very little attention had been given to the study of this group in Iraq; a total of four species are now known from the genus Chaitophorus in Iraq, these species are Ch. euphraticus Hodjat, Ch. leucomelas Koch and Ch. nigritus Hille Ris Lambers (Kaddou, 1966; Daoud and El-Haidari, 1968; Al-Ali, 1977); in addition to Ch. populialbae (Boyer de Fonscolombe, 1841) which recorded here as new record species to aphid fauna of Iraq which are listed in the present study. MATERIALS AND METHODS Aphid specimens infested Euphratic – Poplar Populus euphratica Oliv.; the trees were collected from various locations of Baghdad province, over a period from November 2016 to April 2017. Aphids were collected from their host plants with a fine brush and put in a tube which contained 70% alcohol, the collecting and preserving technique was based mainly on van Emden (1972) method. The aphids were systematically classified according to the catalog of Remaudiére and Remaudiére (1997); all measurements of the aphid are with millimeter (mm),the parameters measured are as in Ilharco and van Harten (1987), except for body length (BL) which is always measured to the base of the Cauda and does not include any projecting cauda. Aphid specimens were deposited in the collection of Iraq Natural History Research Center and Museum, University of Baghdad. RESULTS AND DISCUSSION During the current investigation, four species of the genus Chaitophorus Koch have been recorded to Iraq aphid fauna; the records information for these species is compared with previous checklists as above in addition to our collection; the key to the species of this genus is designed as follows: Key to Chaitophorus species on Iraq popular trees (apterous viviparae in spring): 1- Body oval, color in life dark green, blackish-brown to black……..................… Ch. nigritus - Body short-bodied, to elongate oval, color in life pale green to yellowish white…………..2 2- Dorsum pale or with green irregular markings, siphunculi black…………. Ch. leucornelas - Dorsum with black or dark green longitudinal pleural stripes, siphunculi pale ………...….3 3- Antennal segment VI processus terminalis (ANT PT) / base of antennal segment VI base 2.4-3.0, ultimate rostral segment (URS) 1.0-1.28 × second segment of hind tarsus HT II,……………………………………………………………………….…….Ch. populialbae - Antennal segment VI processus terminalis ANT PT / base of antennal segment VI base 1.5- 2.0, ultimate rostral segment (URS) 0.9-1.0 × second segment of hind tarsus HT II, …………………………………………………...…………………….…Ch. euphraticus Chaitophorus populialbae (Boyer de Fonscolombe, 1841) Diagnostic characters: Apterous viviparous female: color in life pale green to yellowish white, often with small green spots, head and tips of antennal segments and tarsi light brown (Pl. 1) 147 Hayder Badry Ali & Ruia Safwan Kamal Body 1.2-2.4 mm long (Pl. 2A); antennae pale 0.80-1.0 mm long with six segment, 0.50- 0.85 X as long as the body, third antennal segment (ANT III) 0.18-0.30 mm long with blunt or acuminate at apices hairs longest one 0.03- 0.05 mm long. Processus terminalis (ANT PT) 0.22-0.31 mm long, 2.4-3.0 as long as base of antennal segment VI (ANT VI b) (Pl. 2B) which is 0.085-0.12 mm long; rostrum reaches hind coxae, ultimate rostral segment (URS) (Pl. 2C) 0.095-0.138 mm long; 1.0-1.28 X as long as second segment of hind tarsus (HT II) (Pl. 2D) and bears 3-4 accessory hairs. Abdominal dorsum faintly nodulose, hairs on dorsum of abdomen acuminate or furcated at apices; legs pale, hairs on tibiae fine, longest one on hind tibiae 0.070-0.080 mm long, 2.5-3.1 X as long as diameter at middle of hind tibiae, HT II 0.09-0.128 mm long, first tarsal segments with 5, 5, 5 hairs. Siphunculi (Pl. 2E) pale, reticulated; 0.05-0.08 mm long; 0.035- 0.055 X as long as body; cauda (Pl. 2F) pale, distinctly knobbed; 0.08-0.18 mm long; 0.05- 0.08 X as long as body with 6-7 hairs. Plate (1): Ch. populialbae (Boyer de Fonscolombe) color variation in life. Comments: As far as in diagnostic characters between our description of this study and previous literature related to this species, there are many differences, with respect to measurement of body length, Ghosh (1980) determined smaller size than our specimens, as for the length of the antenna, Pintera (1987) described longer antenna, while Ghosh (1980) recorded shorter antenna, and other differences as length of second segment of hind tarsus (HT) that our specimens are significantly longer than that determined by Ghosh (1980) and less clearly by Hodjat (1981). The other characteristic, such as the length of siphunculi and cauda showed minor differences that do not count importantly in taxonomy, and perhaps they are due to geographical variation between different populations of this species. Material examined: sixteen apterous viviparous female and several nymphs, Baghdad province: Al- Jadiriya, 22.XI.2016, 28.II.2017; Taji, 08.III.2016; Abu Ghraib, 25.III.2017; Al- Zaifraniya, 02.IV.2017. 148 Faunistic review of the genus Chaitophorus Koch Global Distribution: Palaearctic region, parts of Africa, and are introduced and widespread in North America (Blackman and Eastop, 1994), this species is new for the Iraqi aphid fauna. Chaitophorus euphraticus Hodjat, 1981 Global Distribution: Iran (Hodjat, 1981), in Iraq Daoud and El-Haidari (1968) recorded this species feed on Euphrates poplar at Baghdad province during April and July. Chaitophorus leucomelas Koch, 1854 (= Chaitophorus versicolor Koch, 1854) Global Distribution: Widely distributed in Europe (Pintera, 1987; Blackman and Eastop, 2015); North Africa (Boukhris-Bouhachem et al., 2007; Laamari et al., 2013); Asia including Kazakhstan, Mongolia, East Siberia (Pashchenko, 1988); North and South America (Rubín de Celis and Ortiz, 1992; Ramirez et al., 2004); in Iraq Daoud and El-Haidari (1968) and Al-Ali (1977) recorded this species on Populus euphratica in Baghdad at August; Diyala and Baquba at October. Chaitophorus nigritus Hille Ris Lambers, 1966 Global Distribution: India (Ghosh, 1980); Pakistan and Iran (Pintera, 1987); Turkey and Iraq (Blackman and Eastop, 2015; Güçlü et al., 2015). Plate (2): Chaitophorus populialbae: (A) Mounted body, (B) Sixth antennal segment (ANT VI), (C) Ultimate rostral segment (URS), (D) Second segment of hind tarsus (HT II), (E) Siphunculi SIHP, (F) Cauda. 149 Hayder Badry Ali & Ruia Safwan Kamal LITEREATURE CITED Al-Ali, A. S. 1977. Phytophagous and entomophagous insects and mites of Iraq. Iraq Natural History Museum, Publication, 33:142pp. Blackman, R. L. and Eastop V. F. 1994. Aphids on the world’s trees. CAB International, Wallingford, 987 pp. Blackman, R. L. and Eastop, V. F. 2015. Aphids on the World’s Plants: An online identification and information guide. Available at: http://www.aphidsonworldsplants.info/ Boukhris-Bouhachem, S., Souissi, R., Turpeau, E., Rouzé Jouan, J., Fahem, M. and Hullé, M. 2007. Aphid (Hemiptera, Aphidoidea) diversity in Tunisia in relation to seed potato production. Annales de la Société Entomologique de France (NS), 43(3): 311–318. Daoud, A. K. and El-Haidari, H. S. 1968. Recorded aphids of Iraq. Iraq Natural History Museum, Publication, 24: 37 pp. Ghosh, A. K. 1980. The fauna of India and the Adjacent Countries. Homoptera, Aphidoidea. Part 1. General Introduction and sub-family Chaitophirinae. Zoological Survey of India, Calcutta, 124 pp. Güçlü, Ş., Kavaz, H., Güçlü, C. and Özdemir, I. 2015. Aphids (Hemiptera: Aphididae) and their parasitoids on ornamental trees and shrubs in Erzurum, Turkey. Türkiye Entomoloji Dergisi, 39 (1): 3-9. Hille Ris Lambers, D. 1960. The genus Chaitophorus Koch in North America (Homoptera, Aphididae). Tijdschrift voor entomologie, 103: 1-30. Hodjat, S. H. 1981. Two new aphids from Iran and keys to related Middle Eastern species. Journal of Natural History, 15: 365-374 Ilharco, F. A. and van Harten, A. 1987. Systematics. pp 51-77. In: Minks, A.K. and Harrewijn, P. (eds.) Aphids, their Biology, Natural Enemies and Control, Vol. A. Elsevier, Amsterdam. Kaddou, I. K. 1966. Aphidae from Iraq. Bulletin of Biological Research Center, 2:21-35. Laamari, M., Coeur d’acier, A. and Jousselin, E. 2013. New data on aphid fauna (Hemiptera, Aphididae) in Algeria. ZooKeys, 319: 223-229 Pashchenko, N. F. 1988. Suborder Aphidinea – aphids. In: Lehr, P.A. (ed.) Keys to the identification of insects of the Soviet Far East Vol. 2, Hemiptera and Heteroptera. 'Nauka', Leningrad, pp 546-686. (In Russian) Pintera, A. 1987. Taxonomic revision of the species of the genus Chaitophorus Koch in Palaearctis (Homoptera: Aphidoidea). Deutsche Entomologische Zeitschrift, 34 (4-5): 219-340. 150 Faunistic review of the genus Chaitophorus Koch Ramírez, C. C., Zamudio, F., Verduco, J. V. and Nuñez, M. E. 2004. Differential susceptibility of poplar hybrids to the aphid Chaitophorus leu (Homoptera: Aphididae). Journal of Economic Entomology, 97: 1965-1971. Remaudière, G. and Remaudière, M. 1997. Catalogue des Aphididae du Monde. INRA, Paris, 473 pp. Rubín de Celis, V. E. and Ortiz, M.S. 1992. Chaitophorus leucomelas Koch (Homoptera: Aphididae, Chaitophorinae) Nuevo registro para Sudamérica. Revista Peruana de Entomologia, 35: 53. Van Emden, H. F. (ed.) 1972. Aphid technology. Academic press, New York, 344pp. Wieczorek, K. 2010. Amonograph of Siphini Mordvilko, 1928 (Hemiptera: Aphidoidea: Chaitophorinae). Wydawnictwo Uniwersytetu Slaskiego, Katowice, 297 pp. Wieczorek, K., Lachowska-Cierlik, D., Kajtoch, È. and Kanturski, M. 2017. The relationships within the Chaitophorinae and Drepanosiphinae (Hemiptera, Aphididae) inferred from molecular-based phylogeny and comprehensive morphological data. PLoS ONE, 12(3): 1-31. Zhu, X-C., Chen, J., Chen, R., Jiang, L-Y. and Qiao, G-X. 2017. DNA barcoding and species delimitation of Chaitophorinae (Hemiptera, Aphididae). ZooKeys, 656: 25–50. 151 Hayder Badry Ali & Ruia Safwan Kamal Bull. Iraq nat. Hist. Mus. December, (2018) 15 (2): 145-151 مراجعة لفونا الجنس Chaitophorus Koch, 1854 (Aphididae, Chaitophorinae, Chaitophorini) مع تسجيل جديد الحد انواع الجنس لفونا الَمن العراقية رؤيا صفوان كمال و حيدر بدري علي .العلوم، جامعة بغداد، بغداد، العراققسم علوم الحياة، كلية 12/52/1522: تاريخ القبول 21/50/1522: تاريخ االستالم الخالصة ، بما في ذلك اربعة Chaitophorus Koch, 1854اجريت مراجعة لفونا الجنس خالل هذا التحري، . بيانات توزيع كل نوع مع مضائفها أنواع في العراق، وقد تم تسجيل Ch. populialbae (Boyer de سجلت حشرة َمن أوراق شجرة الحور Fonscolombe, 1841) على اشجار Populus euphratica Oliv. خالل الفترة من في محافظة بغداد للمرة األولى كإضافة للفونا 6102 إلى نيسان 6102تشرين الثاني . يوانية العراقيةالح اعطي وصف موجز لألناث العذرية غير المجنحة من هذا النوع، مع مفتاح لعزل انواع .في العراق Chaitophorus الجنس