id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
cajgh-165	Kulyyassov, Arman; Zhubanova, Gulsamal; Ramanculov, Erlan; Ogryzko, Vasily	Proximity Utilizing Biotinylation of Nuclear Proteins in vivo	2015	2	.pdf	application/pdf	630	29	28	It has been estimated that over 80% of proteins do not operate alone, but in complexes. Here, A and B are the next nuclear proteins used in the experiments – 3 paralogues of heterochromatin protein HP1 (CBX5), HP1 (CBX1), HP1 (CBX3), wild type and transcription mutant factor Kap1, translesion DNA polymerase PolH and E3, ubiquitin ligase RAD18, Proliferative Cell Nuclear Antigen (PCNA), ubiquitin Ub, SUMO-2/3, different types and isoforms of histones H2A, H2Az, H3.1, H3.3, CenpA, H2A.BBD, and macroH2A. The variant of this approach is termed PUB-NChIP (Proximity Utilizing Biotinylation with Native Chromatin Immuno-precipitation) and is designed to purify and study the protein composition of chromatin in proximity to the nuclear protein of interest.	cache/cajgh-165.pdf	txt/cajgh-165.txt