



































Effect of Probiotic Consortium on the Local Inflammatory Process in Chronic Periodontitis


 

 

New articles in this journal are licensed under a Creative Commons Attribution 3.0 United States License. 

 

 

This journal is published by theUniversity Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

 

 

 

 

 

 
 

 

 

Effect of Probiotic Consortium on 

the Local Inflammatory Process in 

Chronic Periodontitis 

 

Zhanagul Khasenbekova, Saule 

Saduakhasova, Alexandr Gulayev, 

Almagul Kushugulova, Samat 

Kozhakhmetov, Gulnara 

Shakhabayeva, Indira 

Tynybayeva, Talgat Nurgozhin, 

Zhaxybay Zhumadilov 
 
Center for Life Sciences, Nazarbayev 
University, Astana, Kazakhstan 

 

 

Vol. 2, Suppl. (2013)   |   ISSN 2166-7403 (online)  

DOI 10.5195/cajgh.2013.109  |   http://cajgh.pitt.edu 

http://www.library.pitt.edu/
http://www.pitt.edu/
http://www.library.pitt.edu/articles/digpubtype/index.html
http://www.upress.pitt.edu/upressIndex.aspx
http://creativecommons.org/licenses/by/3.0/us/


 

 

KHASENBEKOVA 

 

 

This work is licensed under a Creative Commons Attribution 3.0 United States License. 

 

This journal is published by theUniversity Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 2, Suppl. (2013)  |  ISSN 2166-7403 (online) |  DOI 10.5195/cajgh.2013.109  |  http://cajgh.pitt.edu 

 

 

Abstract 

Introduction: Inflammatory periodontal disease is one of the major concerns of researchers and clinicians, because it can lead to 

tooth loss and an increased risk of systemic pathologies, even at the age of 35. The purpose of this study was to determine the 

effects of gelatin-based probiotic consortium on the local and general factors of inflammation in rats with chronic periodontitis. 

Methods: The study object was a complex of probiotic bacteria based in an odourless 6% gelatin plate with neutral flavour. A 

cellular biomass of the consortium consists of following lactobacilli: Lactobacillus casei subsp. pseudoplantarum, Lactobacillus 

caseisubsp.casei, L.fermentum, and L. helveticus. The viable cell number was 2.5 x 109 CFU/ml. The model of chronic 

periodontitis was reproduced in the white random-bred rats that weighed 160-220g, by keeping them on a low-protein diet. After 

three months, symptoms associated with medium and severe chronic periodontitis were observed in the rats. Application was 

carried out on the oral mucosa of rats 1 time per day for 14 days. The stickers lacking consortium of microorganisms were used 

as the placebo. The "Solcoseril" gel was chosen as a comparator. The hematologic, biochemical, and morphological 

characteristics were investigated.  

Results: A complete clearance of periodontal pockets was observed during an objective examination of the experimental group 

rats on the 14th day of the experiment. Moreover, a gingival mucous turned pink, and there were no cyanosis tissues. The local 

changes were accompanied by improvement in hematological parameters, such as a reduction of blood eosinophilia and 

neutrophilia, and a recovery of the white blood cells number to the normal degree within the group that received the probiotic 

complex. A decrease of the acute plethora of microvasculature was observed morphologically as a result of the treatment. There 

were signs of basal layer activation of the stratified squamous epithelium with a merger of the acanthosis outgrowths and a 

formation of the fibrotic nodules. Biochemical investigations did not show significant changes in the indicators. 

Conclusions: In the settings of the chronic periodontitis model, the use of gelatin-based probiotic consortium consisting of 

Lactobacillus casei subsp. pseudoplantarum, Lactobacillus caseisubsp.casei, L.fermentum, L. helveticus. at 2.5 x 109 CFU/ml 

viable cell numbers lead to the reduction of the local inflammatory manifestations of the periodontitis in 14 days of treatment. 

Keywords: probiotics, chronic periodontitis, lactobacilli 

 

http://www.library.pitt.edu/
http://www.pitt.edu/
http://www.library.pitt.edu/articles/digpubtype/index.html
http://www.upress.pitt.edu/upressIndex.aspx

