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Effects of Antioxidants and 

Vitamins on the Proliferation of 

Human Diploid Cells 

 

Gaziza Dаnlybaeva, Assel 

Isabekova, Zhansaya 

Akhmadeyeva 

 

National Center for Biotechnology, 

Astana, Kazakhstan 

 

Vol. 2, Suppl. (2013)   |   ISSN 2166-7403 (online)  

DOI 10.5195/cajgh.2013.112  |   http://cajgh.pitt.edu 

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DANLYBAEVA 

 

 

This work is licensed under a Creative Commons Attribution 3.0 United States License. 

 

This journal is published by theUniversity Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 2, Suppl. (2013)  |  ISSN 2166-7403 (online) | DOI 10.5195/cajgh.2013.112 | http://cajgh.pitt.edu 

 

 

Abstract 

Introduction: Microelements, essential nutrients that are needed in small amounts including minerals such as calcium, zinc, iron 

and other vitamins (A, B, C, and etc.), are macronutrients necessary for a healthy life. 

 

The role of micronutrients in vivo is well known, and there are several publications that have examined the effects of 

micronutrients on genomic stability. Furthermore, a number of vitamins and microelements are substrates and/or cofactors in 

metabolic pathways, which regulate DNA synthesis and/or repair and gene expression. 

 

A deficiency in such nutrients may result in disruption of genomic integrity and alterations in DNA methylation patterns, linking 

cellular nutrition with change in gene expression. For example, lack of vitamin C is known to cause increased DNA oxidation 

and chromosomal damage. Vitamin A, as well as other micronutrients, have a protective effect, whereas higher concentrations 

are associated with increased DNA damage. 

 

Ubiquinone (coenzyme Q10) and dihydroquercetin are used in therapy as antioxidant compounds and electron carriers, which 

reduce lipid peroxidation of cell membranes. However, previous studies indicate that various ubiquinone analogs may cause a 

divergent effect on oxidative stress and oxidative phosphorylation. 

 

The aim of our study was to investigate the effect of vitamins A and C, coenzyme Q10, and dihydroquercetin on the proliferative 

potential of cultured human embryonic diploid fibroblasts (M-22). 

 

Methods: In the first series of experiments, nontoxic concentrations of vitamins for the cells were identified using MTT assay.  

 

Results: Vitamins A and C, dihydroquercetin of 1µM, and coenzyme Q10 of 5µM were nontoxic for human skin fibroblasts. 

In the second series of experiments, cell cultivation was carried out with nontoxic concentrations. A vitamin C concentration of 

1µM for 7 consecutive passages increased the proliferation index (PI) compared to the control. Thus, the average PI in the 

experiments was 2.3, whereas in the control, it was 1.7. Similar results were obtained when dihydroquercetin was added to the 

growth medium. However, further cultivation of cells in the presence of vitamin C decreased PI to 1.4, while the control value 

remained the same. Daily examination revealed no morphological changes in the cell culture, but the cell growth had slowed 

significantly. The use of vitamin A in a nontoxic concentration of 1 µM reduced PI to 0.7 in the first passage, so further culturing 

of human cells with vitamin A was stopped.  

 

Conclusion: Studies examining the effect of different combinations of microelements on the proliferation of human diploid cells 

and the expression of specific proteins in them are still being conducted. 

Keywords: vitamins, antioxidants, human diploid cells 

 

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