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of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

 

 

 

 

 

 
 

 

 

 

 

Antioxidant activity of the 

probiotic consortium in vitro 

 

Saule Saduakhasova, Almagul 

Kushugulova, Samat 

Kozhakhmetov, Gulnara 

Shakhabayeva, Indira 

Tynybayeva, Talgat Nurgozhin, 

Zhaxybay Zhumadilov 

 

Center for Life Sciences, Nazarbayev 

University 

 

Vol. 2, Suppl. (2013)   |   ISSN 2166-7403 (online)  

DOI 10.5195/cajgh.2013.115  |   http://cajgh.pitt.edu 

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SADUAKHASOVA 

 

 

This work is licensed under a Creative Commons Attribution 3.0 United States License. 

 

This journal is published by theUniversity Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 2, Suppl. (2013)  |  ISSN 2166-7403 (online) |  DOI 10.5195/cajgh.2013.115 |  http://cajgh.pitt.edu 

 

 

Abstract 

Introduction: Available evidence suggests that probiotics have different biological functions that depend on several 

mechanisms, such as antioxidant and DNA-protective activities. The probiotic consortium includes bacterial cultures such as 

Streptococcus thermophilus, Lactococcus lactis, Lactobacillus plantarum, and other bacterial cultures isolated from traditional 

Kazakh dairy products (ayran, kumys, shubat, and healthy clinical material). The aim of this study was to investigate the total 

antioxidant activity of the consortium of probiotic bacteria and to determine the activity of superoxide dismutase, glutathione 

reductase, and DNA-protective action. 

 

Material and methods: In vitro comet assay was used to determine the antigenotoxicity of the probiotic consortium. Total 

antioxidant activity was determined using a method of analysis with Trolox as the equivalent. The analysis method of superoxide 

dismutase activity assesses the inhibition rate of the nitroblue tetrazolium reduction to formazan by superoxide dismutase. 

Determination of glutathione reductase activity is based on the measurement of the NADPH oxidation speed. 

 

Results: A significantly high level of the total antioxidant activity of the probiotic consortium intact cells (15.3 mM/ml) was 

observed whereas the activity index of  lysate  was 11.1 mM/ml. 

 

The superoxide dismutase activity of probiotic consortium lysate was evaluated, with values that peaked at 0.24 U/mg protein. 

The superoxide dismutase activity of the consortium was lower in comparison to L.fernentum E-3 and L.fernentum E-18 cultures 

with values of 0.85 U/mg and 0.76 U/mg protein, respectively. SOD activity of probiotic consortium whole cells was not 

observed, which is typical for lactic acid bacteria. 

 

Glutathione reductase plays an important role in the optimal protection from oxidative stress. Glutathione reductase activity of 

the studied probiotic consortium was low; moreover, the activity of the lysate was two times higher than the activity of the cells 

reaching 0.01 units/ml. Investigations by Dr. Li have shown that the intracellular glutathione may give a significant protection of 

Lactococcus from the damaging action of H2O2, even at very low concentrations. 

 

The data from our study suggests that the co-incubation of the epithelial cells with probiotic bacteria reduces the percentage of 

damaged cells (damage index–0.60). 

 

Conclusion: The studied probiotic consortium has antigenotoxic and antioxidant activities. Preparations and products of this 

probiotic consortium may serve as a protective component in the intestinal microbial ecosystem. 

Keywords: antioxidant activity, probiotic bacteria 

 

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