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of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

 

 

 

 

 

 

 

 

 

Influence of Probiotic Consortium 

on TH1 and TH2 Immune 

Response 

 

 

Gulnara Shakhabayeva, Almagul 

Kushugulova, Saule 

Saduakhasova, Samat 

Kozhakhmetov, Zhanagul 

Khasenbekova, Indira 

Tynybayeva, Talgat Nurgozhin, 

Zhaxybay Zhumadilov 
 

Center for Life Sciences, Nazarbayev 

University, Astana, Kazakhstan 

 

 

Vol. 2, Suppl. (2013)   |   ISSN 2166-7403 (online)     

DOI 10.5195/cajgh.2013.122   |   http://cajgh.pitt.edu 

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SHAKHABAYEVA 

 

 

This work is licensed under a Creative Commons Attribution 3.0 United States License. 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 2, Suppl. (2013)  |  ISSN 2166-7403 (online) |  DOI 10.5195/cajgh.2013.122 |  http://cajgh.pitt.edu 

  

 

Abstract 

Introduction: The main role of probiotics is to maintain homeostasis in the intestines and improve bowel protective function. 

The aim of the present study is to investigate immuno-modulatory effects of a probiotic consortium. 

 

Methods: Observations were carried out in vitro. The presence of IL-2, IL-4, IL-6, IL-8, IL-10, TNF-α, IFN-γ, IgA, IgM, and 

IgE was studied using a solid-phase enzyme immunosorbent assay on the VECTOR-BEST sets (Russia). 

 

Results: Immunomodulatory properties of the probiotic consortium were studied, which consisted of the following strains: 

Streptococcus thermophilus, Lactococcus lactis, Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus acidophilus, 

Bifidobacterium longum, and Bifidobacterium bifidum. Elevated concentrations of INFγ in control samples decreased 3.9 times 

(p < 0.05) after a saturation of blood with the probiotic consortium. Significant reduction of cytokine levels after the probiotic 

effects of the consortium was observed in IL-10 by 2.1 times (p < 0.05) and IgA by 1.87 times (p < 0.0005). There was a 

significant increase in the levels of IL-4, IgE, IL-6, and IL-8 by 1.3 (p < 0.005), 1.1 (p < 0.5), 18.0 (p < 0.005), and 6 (p < 0.05) 

times, respectively, in comparison with the control samples. IL-4 and INFγ have different effects on the synthesis of IgE. Soluble 

low affinity receptors FcεRII (CD23) in association with IL-4 facilitate a differentiation of the B-lymphocytes in IgE-

synthesizing cells, while γ-INF inhibits this process. It is known that the intracellular expression of γ-INF and IL-4 is the most 

reliable marker for Th1 and Th2 immune responses, respectively. The conducted studies determined that the ratio of INF-γ/IL-4 

was 0.9 (control 4.8, P < 0.005) after the saturation of the blood cells with probiotic consortium. NF-γ/IL4 ratio decreased by 5.3 

times compared with a control value, which indicates a reduction in the functional activity of Th1 type lymphocytes in 

comparison with the function of Th2 cells. 

  

Conclusion: The application of the probiotic consortium results in the maintenance of homeostasis by the stimulation of immune 

function through the activation of humoral immunity. Moreover, the probiotic application changes the orientation of the 

immunological memory causing the cancellation of the recruitment of Th1 cells in the response.  

Keywords: probiotics, immune response, immunity 

 

 

 

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