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New articles in this journal are licensed under a Creative Commons Attribution 4.0 United States License. 

 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

 

 

 

 

 

 
 

 

Transplantation of Cultivated 

Fibroblasts on a Backing of 

Xenogenic Tissue in the 

Treatment of Wounds 

 

Kabylbek Abugaliyev1, Ogay 

Vyacheslav2, Danlybayeva 

Gaziza2, Miras Karzhauov2, 

Akhmadeyeva Zhansaya2, Anuar 

Zhaikenov1  

 
1Republican Scientific Center for 
Emergency Care, Astana, Kazakhstan; 
2National Center for Biotechnology, 
Astana, Kazakhstan 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Vol. 3, Suppl. (2014)   |   ISSN 2166-7403 (online)    

DOI 10.5195/cajgh.2014.143   |   http://cajgh.pitt.edu 

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ABUGALIYEV 

 

 

This work is licensed under a Creative Commons Attribution 4.0 United States License. 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 3, Suppl. (2014)  |  ISSN 2166-7403 (online)  |  DOI 10.5195/cajgh.2014.143  |  http://cajgh.pitt.edu 

  

 

Abstract 

Introduction. Trophic ulcers are a common health problem, and there are numerous treatment methods. Irreversible damage in the 

skin, subcutaneous tissue, and fascia with long-term ulcer existence make standard autotransplantation inneffective. Skin grafts are 

often complicated by partial or complete rejection of skin flaps. The aim of this study was to examine the feasibility of using 

transplanted cultivated allogenic fibroblasts on the backing of a cellularless xenogenic fabric for wound healing. 

Methods. Transplantation of cultured embryonic fibroblasts on a backing of xenogenic tissue was used in the complex treatment 

of trophic ulcers for stimulation of regenerative processes. Decellularization xenogenic film was previously held. Then allogenic 

fibroblasts were cultivated on the surface of collagen-elastin matrix. Since 2013, we treated 12 patients with giant ulcers caused by 

the following: lymphedema (2 patients), vascular disease (3 patients), diabetes (2 patients), after injury (4 patients), and radiation 

ulcer (1 patient). Dimensions of ulcers were from 150 to 600 cm2. Duration of the lower limb ulcers ranged from 8 months to 10 

years. For a number of years, all patients were on a complex therapy, which had not resulted in healing wounds. During the operation 

when excision of granulation tissue was performed, plastic wounds perforated with the ratio 1:2 autoskin. Xenogenic fabric with 

cultured fibroblasts was applied on top. In this case, xenogenic film protected the skin from drying, created optimal microclimate, 

and cultured fibroblasts stimulating regeneration and improving engraftment. 

Results. The first redress was held on the fifth day. In all cases, the results of engraftment skin grafts achieved maximum possible 

(100%) and optimal (90%). Complete epithelialization of the cell perforation was seen in five patients on the fifth day and three on 

seventh day after skin plastics. Average period of inpatient treatment was 20.7 days. All patients were discharged with healed 

wounds.  

Conclusion. Thus, the treatment of trophic ulcers can be successfully solved using advances in biotechnology. Transplantation of 

cultivated allogenic fibroblasts on a backing of cellularless xenogenic fabric shows good clinical results due to the stimulation of 

regenerative processes and creates the optimum environment for autotransplants. 

Keywords: transplantology, tissue engineering, fibroblasts  

 

 

 

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