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New articles in this journal are licensed under a Creative Commons Attribution 4.0 United States License. 

 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

 

 

 

 

 

 
 

 

 

 

Clinical Experience with Chitosan 

Matrix and Cultured Fibroblasts 

for Burns  

  

Gaziza Danlybayeva1, Assylbek 

Zhylkibayev1, Zhansaya 

Akhmadeyeva1, Elena Belan2, 

Zhanatay Ramazanov2 

 
1Laboratory of Stem Cells, National 
Center for Biotechnology, Astana, 
Kazakhstan; 2Scientific Research 
Institute of Traumatology and 
Orthopedics, Astana, Kazakhstan 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Vol. 3, Suppl. (2014)   |   ISSN 2166-7403 (online)    

DOI 10.5195/cajgh.2014.157   |   http://cajgh.pitt.edu 

 

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DANLYBAYEVA 

 

 

This work is licensed under a Creative Commons Attribution 4.0 United States License. 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 3, Suppl. (2014)  |  ISSN 2166-7403 (online)  |  DOI 10.5195/cajgh.2014.157  |  http://cajgh.pitt.edu 

  

 

Abstract 

Introduction. Burns are an important public health challenge due to the frequency of getting burns in day-to-day life, occupational 

hazards, and catastrophes. Treatment of burns is complex and is associated with high morbidity and mortality. Duration and 

complexity of burn treatment require finding new ways of curing and rehabilitating burns. The result of burn treatment plays a 

significant role in post-traumatic status of a patient and his or her consequent adaptation in society. Chitosan is a natural safe non-

toxic product compatible with human tissues, characterized by hydrosorbid, anticoagulant, antibacterial, and wound healing 

features. The study aims to  show a clinical application of chitosan-pectin scaffold with cultured human skin fibroblasts in the 

treatment of deep burns. 

Methods. The substrate was prepared by dissolving 3% chitosan in 0.5N acetic acid, which was then mixed with 3% solution of 

pectin dissolved in distillated water. Chitosan film was formed in a Petri dish for 20-24 hours at 20-25 °C. After drying the film, 

cultured allogeneic fibroblasts (patent number RK-25091) were seeded on its surface. 

Results. The results from an in vitro culture study showed that human allogeneic fibroblasts could adhere well and grow on the 

selected scaffold with a typical morphology. During autodermoplasty surgery, cultured allogeneic fibroblasts were applied on 

granulating wounds of 9 patients with IIIA to IVB degree burns and limited donor resources. Wounds treated with the fibroblast-

seeded scaffold among all patients provided the highest level of re-epithelialization (day 5), in comparison to cell-free scaffold (day 

7) and untreated surface of wounds (day 10).  

Conclusion. Our results indicate the potential use of chitosan for wound healing due to its allogenic fibroblast adherence to 

scaffolding as well as high epithelization. This warrants further studies on chitosan for use in wounds resulting from third and 

fourth degree burns. 

Keywords: burns, chitosan-pectin scaffold, fibroblast, epithelialization 

 

 

 

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