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This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

 

 

 

 

 

 

 

Antimutagenic Properties of 

Biologically Active Substances of 

Microalgae Associates  

 

Saule Kolumbayeva1, Saule 

Dzhokebayeva2, Dinara 

Begimbetova3, Anna Lovinskaya2 

 
1Department of Biology and 
Biotechnology, Al-Farabi Kazakh National 
University, Almaty, Kazakhstan; 2Ecology 
Institute, Al-Farabi Kazakh National 
University, Almaty, Kazakhstan; 3Center 
for Life Sciences, Nazarbayev University, 
Astana, Kazakhstan 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  

 

 

 

Vol. 3, Suppl. (2014)   |   ISSN 2166-7403 (online)    

DOI 10.5195/cajgh.2014.162   |   http://cajgh.pitt.edu  

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KOLUMBAYEVA 

 

 

This work is licensed under a Creative Commons Attribution 4.0 United States License. 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 3, Suppl. (2014)  |  ISSN 2166-7403 (online)  |  DOI 10.5195/cajgh.2014.162  |  http://cajgh.pitt.edu 

  

 

Abstract 

Introduction: There are an increasing number of different xenobiotics negatively influencing population health. Therefore, it is 

important to find effective protectors against mutagenic and toxic effects of environmental pollutants. Naturally occurring 

biologically active substances, the majority of which are antioxidants, are capable of functioning as modifiers of the induced 

mutation process. The application of various naturally occurring protectors will lower essential risks of congenital malformations, 

cancer, and hereditary diseases caused by mutational damages. Therefore, it is crucial to screen algal flora of Kazakhstan for the 

antimutagenic activity. This study involved the assessment of antimutagenic potential of biologically active polypeptide (BAP) 

produced in mixed microalgae cultures.  

Methods. 70 white outbred male rats (Rattus norvegicus) at 6 months of age were used for this study. The dosage of BAP produced 

by microalgae associates Anabaena flos-aquae x Anabaenopsis sp. comprised 100 mg/kg. Cadmium sulfate was used as a mutagen 

in a concentration of 1 mg/kg. Experiments on antimutagenic activity of BAP were carried out with the Mammalian Bone Marrow 

Chromosomal Aberration Test. 

Results. After acute and subacute exposure of BAP, the level of chromosomal structural abnormalities in rat bone marrow cells 

was the same as in control group. Therefore, BAP showed no mutagenic activity, whereas exposure to cadmium sulfate at used 

concentration induced chromosomal aberrations with a significantly higher frequency than the spontaneous mutation rate. The 

exposure combination of BAP with cadmium sulfate resulted in a two-fold decrease (р < 0.05) of mutagen-induced chromosomal 

aberrations. The range of induced chromosomal aberrations included alterations of all types both in control and experimental 

groups.  

Conclusion. Most of the genotoxic effects are mediated through oxidative stress. The repair of DNA damage is an enzymatic 

process, which depends on the cellular metabolic rate. It has previously been shown that many biologically active substances lead 

to reduction of DNA sensitivity to mutagenic damaging factors. Based on these facts and obtained results, it can be assumed that 

BAP from mixed microalgae cultures Anabaena flos-aquae х Anabaenopsis sp. are capable of blocking free radical process reducing 

the likelihood of genome damage, as well as triggering the cellular repair system.   

Keywords: natural protectors, microalgae, biologically active polypeptide, antimutagenic activity 

 

 

 

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