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Lactobacillus for Vaginal 

Microflora Correction 

 

Saule Saduakhasova, Almagul 

Kushugulova, Gulnara 

Shakhabayeva, Samat 

Kozhakhmetov, Zhanagul 

Khasenbekova, Indira 

Tynybayeva, Talgat Nurgozhin, 

Zhaxybay Zhumadilov 

 
Center for Life Sciences, Nazarbayev 
University, Astana, Kazakhstan 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Vol. 3, Suppl. (2014)   |   ISSN 2166-7403 (online)    

DOI 10.5195/cajgh.2014.171   |   http://cajgh.pitt.edu 

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SADUAKHASOVA 

 

 

This work is licensed under a Creative Commons Attribution 4.0 United States License. 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 3, Suppl. (2014)  |  ISSN 2166-7403 (online)  |  DOI 10.5195/cajgh.2014.171  |  http://cajgh.pitt.edu 

  

 

Abstract 

Introduction. Despite the significant progress made in prevention, diagnosis, and treatment, there is still a high rate of vaginal 

dysbiosis in Kazakh women. The use of antibiotics in the treatment of vaginal dysbiosis contributes to the elimination of pathogens 

as well as microflora, which can lead to a decrease in local immunity and more favorable conditions for infection spread. The most 

physiologically safe and promising method for the restoration of vaginal biocenosis is the use of probiotics administered by a 

vaginal route.  

Methods. We have allocated 64 of cultures of Lactobacillus from the vaginal epithelium of healthy women of reproductive age 

and women with diagnosed bacterial vaginosis (BV). Identification of cultures was performed by PCR analysis of 16S ribosomal 

RNA.  Evaluation of biological significance was determined by the following criteria: high antagonistic activity against Candida 

albicans, Escherichia coli, Serratia marcescens, Proteus mirabilis, Klebsiella ozaenae, and Staphylococcus aureus; and production 

of hydrogen peroxide, resistance to antibiotics, adhesive activity. We studied the symbiotic relationship of selected biologically 

active of cultures to each other and received options for consortiums with  properties of  probiotics through co-cultivation. 

Results. Results of genotyping  showed that the isolated lactobacilli belong to the seven species: L. fermentum, L. salivarius, L. 

gasseri, L. crispatus, L. jensenii, L. plantarum, and L. delbrueskii. L. fermentum, L. salivarius, L. gasseri, and L. jensenii occur in 

women with suspected BV. The highest percentage of occurrence in the vagina of healthy women was L. fermentum (28%). Most 

strains of lactobacilli possess high inhibitory activity for all test-strains, except Candida albicans (37.5%). 56% of studied cultures 

revealed high adhesion to human erythrocytes. All lactobacillus strains were resistant to metronidazole, 80% to kanamycin, 57%  

to vancomycin, and sensitivity to roxithromycin, amoxiclav, ampicillin was diagnosed in all strains. 50% of cultures showed a 

moderate sensitivity to gentamicin and cefazolin.  In a study of peroxide-producing activity, 80% of the cultures exhibited peroxide-

producing activity. As a result of sсreening, the 7 most active strains of lactobacilli were selected for development of 10 variants 

of probiotic consortia. Also, there was increase of  adhesive activity in the consortia compared to other components. These consortia 

can be used for the treatment of BV in addition to metronidazole. 

Conclusion. The probiotic consortia identified in this study had high antagonistic, adhesive properties, and resistance to 

metronidazole. These probiotics can potentially be used for the development of biological products for the treatment and prevention 

of bacterial vaginosis. 

Keywords: vaginal microflora, lactobacillus, bacterial vaginosis, probiotics 

 

 

 

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