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New articles in this journal are licensed under a Creative Commons Attribution 4.0 United States License. 

 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

 

 

 

 

 

 
 

 

 

 

PTH Gene Polymorphism and 

Breast Cancer Risk in Kazakhstan 

 

Nurgul Sikhayeva1, Zhannur 

Abilova2, Ivan Shtefanov3, Abai 

Makishev3, Ainur Akilzhanova2 

 
1National Center for Biotechnology, 
Astana, Kazakhstan; 2Center for Life 
Sciences, Astana, Kazakhstan; 
3Department of Oncology, Astana 
Medical University, Kazakhstan 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Vol. 3, Suppl. (2014)   |   ISSN 2166-7403 (online)    

DOI 10.5195/cajgh.2014.175   |   http://cajgh.pitt.edu 

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SIKHAYEVA 

 

 

This work is licensed under a Creative Commons Attribution 4.0 United States License. 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 3, Suppl. (2014)  |  ISSN 2166-7403 (online)  |  DOI 10.5195/cajgh.2014.175  |  http://cajgh.pitt.edu 

  

 

Abstract 

Introduction. Breast cancer is the most common type of cancer among women. In Kazakhstan, breast cancer holds first place 

among causes of women death caused by cancer in the 45-55 year age group . Many studies have shown that the risk of acquiring 

breast cancer may be related to the level of calcium in the blood serum. One of the important regulators of calcium metabolism in 

the body is the parathyroid hormone. Single nucleotide polymorphisms in the gene encoding the parathyroid hormone (PTH) are 

associated with breast cancer development risk, and may modify the associative interaction between the levels of calcium intake 

and breast cancer. Experimental studies have shown that PTH gene has a carcinogenic effect. At least three studies showed a weak 

positive correlation between the risk of acquiring breast cancer and primary hyperparathyroidism, a state with high levels of PTH 

and often high levels of calcium. The aim of this investigation was to evaluate potential association between PTH gene 

polymorphism and breast cancer risk among Kazakhstani women. 

Methods. Female breast cancer patients (n = 429) and matched control women (n = 373) were recruited into a case – control study,. 

Genomic DNA was extracted from peripheral venous blood of study participants using Wizard® Genomic DNA Purification Kit 

(Promega, USA). Detection of PTH gene polymorphism (rs1459015) was done by means of the TaqMan® SNP Genotyping Assay 

of real-time PCR. Statistical analysis was conducted using SPSS 19.0. 

Results. PTH gene alleles were in Hardy–Weinberg equilibrium (p > 0.05). Distribution was 59% CC, 35% CT, 6% TT in the 

group with breast cancer and 50% CC, 43% CT, 6% TT in the control group. Total difference (between the group with breast cancer 

and the control group) in allele frequencies for PTH polymorphism was not significant (p > 0.05). No association was found 

between rs1459015 TT and breast cancer risk (OR = 1.039; 95%, CI 0.740 - 1.297; p = 0.893). 

Conclusion. We found no association between PTHrs1459015 polymorphism and breast cancer in our present study. Further 

studies are required to confirm our results and clarify role of PTH gene genotypes on breast cancer risk. 

Keywords: breast cancer, parathyroid hormone gene, polymorphism 

 

 

 

http://www.library.pitt.edu/
http://www.pitt.edu/
http://www.library.pitt.edu/articles/digpubtype/index.html
http://www.upress.pitt.edu/upressIndex.aspx
https://www.promega.com/resources/protocols/technical-manuals/0/wizard-genomic-dna-purification-kit-protocol/
https://www.promega.com/resources/protocols/technical-manuals/0/wizard-genomic-dna-purification-kit-protocol/
http://www.lifetechnologies.com/kz/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/snp-genotyping-taqman-assays/single-tube-snp-genotyping.html?ICID=search-product

