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Fetal Nerve Cell Transplantation in 

Early Post-Resuscitation Period in 

Rats 

 

Damira Tazhibayeva1, Farkhad 

Olzhayev2, Natalya 

Kadbudalieva1, Zhaina Aitbayeva1, 

Lina Zaripova1 

 
1Department of Pathological Physiology, 
Astana Medical University, Astana,  
Kazakhstan; 2Center for Life Sciences, 
Nazarbayev University, Astana, 
Kazakhstan 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Vol. 3, Suppl. (2014)   |   ISSN 2166-7403 (online)    

DOI 10.5195/cajgh.2014.178   |   http://cajgh.pitt.edu 

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TAZHIBAYEVA 

 

 

This work is licensed under a Creative Commons Attribution 4.0 United States License. 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 3, Suppl. (2014)  |  ISSN 2166-7403 (online)  |  DOI 10.5195/cajgh.2014.178  |  http://cajgh.pitt.edu 

  

 

Abstract 

Introduction. Fetal cell transplantation is a promising biomedical approach for disease treatment; however, the use of fetal cell 

therapy is still experimental. This research was deemed a necessity to provide evidence-based research for the application of cell 

transplantation as a treatment method. The aim of this study was to evaluate the effect of fetal nerve cell transplantation in rat 

survivors (and non-survivors) after clinical death by mechanical asphyxia. 

Methods. 68 white laboratory rats were divided into two groups of identical age and sex: a control group of 12-month adult male 

rats (n = 26) and an experimental group (n = 42). Rats were fixed under ether anesthesia. We then blocked the oral and nasal regions 

with cotton wool soaked in saline solution. A four-minute clinical death though acute mechanical asphyxia was simulated by 

applying the method of N. Shim. After the 4-minute clinical death, we resuscitated the rats using external cardiac massage and 

artifical respiration. Suspension of the fetal nerve cells was injected intraperitoneally at 1mm3 per 25g at the time of cardiac activity 

restoration. Lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) levels were examined in the homogenate cerebral 

cortex of reanimated animals. We recorded the survival rate during the post-resuscitation period and analyzed the integrative brain 

functions using anxiety-phobic status and latent inhibition.  

Results. After fetal nerve cell transplantation, the enzymatic reactions in the experimental group became normal with a significant 

decrease in LDH and an increase in CPK levels compared to the control group. In the control group, 10 rats died and 16 lived (62% 

survival rate), while 7 rats died and 35 lived (83% survival rate) in the experimental group during the first 7 days. Rats that did not 

receive the treatment tended to die sooner than those in the experimental group. As a result of transplantation, the anxiety level in 

the experimental group was less than in the control group. Moreover, cell therapy improved the reflexes in the experimental animals.  

Conclusions. The study revealed the positive neuroprotective effect of the fetal nerve cells on the recovery in the early post-

resuscitation period. This was confirmed by the normalization of enzymatic reactions, improvement reflective activity, and increase 

in the survival rate of the resuscitated animals in the group treated with fetal nerve cell transplantation. These findings warrant 

future research on the mechanisms associated with reflex improvement.  

Keywords: fetal nerve cell transplantation, survival, resuscitation  

 

 

 

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