Complete Genome Sequence of the Probiotic Lactic Acid Bacterium Lactobacillus Rhamnosus


 

 

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Complete Genome Sequence of 

the Probiotic Lactic Acid 

Bacterium Lactobacillus 

Rhamnosus 

 

Samat Kozhakhmetov1, Almagul 

Kushugulova1, Adil Supiyev1, 

Indira Tynybayeva1, Ulykbek 

Kairov1, Saule Saduakhasova1,  

Gulnara Shakhabayeva1, 

Kenzhebulat Bapishev2, Talgat 

Nurgozhin1, Zhaxybay 

Zhumadilov1 

 
1Center for Life Sciences, Nazarbaev 

University, Astana, Kazakhstan  
2Medical Social Institution for Older 
People and Invalids, Astana, Kazakhstan 

 

Vol. 2, Suppl. (2013)   |   ISSN 2166-7403 (online)  

DOI 10.5195/cajgh.2013.113  |   http://cajgh.pitt.edu 

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KOZHAKHMETOV 

 

 

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This journal is published by theUniversity Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 2, Suppl. (2013) |  ISSN 2166-7403 (online) | DOI 10.5195/cajgh.2013.113 | http://cajgh.pitt.edu 

 

 

Abstract 

Introduction: Lactobacilli are a bacteria commonly found in the gastrointestinal tract. Some species of this genus have probiotic 

properties. The most common of these is Lactobacillus rhamnosus, a microoganism, generally regarded as safe (GRAS). It is also 

a homofermentative L-(+)-lactic acid producer. The genus Lactobacillus is characterized by an extraordinary degree of the 

phenotypic and genotypic diversity. However, the studies of the genus were conducted mostly with the unequally distributed, 

non-random choice of species for sequencing; thus, there is only one representative genome from the Lactobacillus rhamnosus 

clade available to date. The aim of this study was to characterize the genome sequencing of selected strains of Lactobacilli.  

 

Methods: 109 samples were isolated from national domestic dairy products in the laboratory of Center for life sciences. After 

screaning isolates for probiotic properties, a highly active Lactobacillus spp strain was chosen. 

Genomic DNA was extracted according to the manufacturing protocol (Wizard® Genomic DNA Purification Kit). The 

Lactobacillus rhamnosus strain was identified as the highly active Lactobacillus strain accoridng to its morphological, cultural, 

physiological, and biochemical properties, and a genotypic analysis. 

 

Results: The genome of Lactobacillus rhamnosus was sequenced using the Roche 454 GS FLX (454 GS FLX) platforms. The 

initial draft assembly was prepared from 14 large contigs (20 all contigs) by the Newbler gsAssembler 2.3 (454 Life Sciences, 

Branford, CT). 

 

Conclusion: A full genome-sequencing of selected strains of lactic acid bacteria was made during the study. 

Keywords: lactobacilli, lactic acid bacteria, genome sequencing

 

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