Whole genome sequencing of M.tuberculosis in Kazakhstan: preliminary data


 

 

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Whole genome sequencing of 

M.tuberculosis in Kazakhstan: 

preliminary data 

 

 

Ulykbek Kairov1, Ulan 

Kozhamkulov1, Saule Rakhimova1, 

Ayken Askapuli1, Maxat 

Zhabagin1, Venera Bismilda2, 

Leyla Chingissova2, Zhaxybay 

Zhumadilov1, Ainur Akilzhanova1 

 
 

1Center for Life Sciences, Nazarbayev 

University, Astana, Kazakhstan; 
2National Center for Problems of 

Tuberculosis, Almaty, Kazakhstan  

 

 

Vol. 2, Suppl. (2013)   |   ISSN 2166-7403 (online)     

DOI 10.5195/cajgh.2013.121   |   http://cajgh.pitt.edu 

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KAIROV 

 

 

This work is licensed under a Creative Commons Attribution 3.0 United States License. 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 2, Suppl. (2013)  |  ISSN 2166-7403 (online) |  DOI 10.5195/cajgh.2013.121 |  http://cajgh.pitt.edu 

  

 

Abstract 

Background: Tuberculosis is a major public health problem which infects one third of the world’s population, 

resulting in more than two million deaths every year. The emergence of whole genome sequencing (WGS) 

technologies as a primary research tool has allowed for the detection of genetic diversity in Mycobacterium 

tuberculosis (MTB) with unprecedented resolution. WGS has been used to address a broad range of topics, including 

the dynamics of evolution, transmission, and treatment. To our knowledge, studies involving WGS of Kazakhstani 

strains of M. tuberculosis have not yet been performed. 

 

Aim: To perform whole genome sequencing of M. tuberculosis strains isolated in Kazakhstan and analyze sequence 

data (first experience and preliminary data). 

 

Results: In the present report, we announce the whole-genome sequences of the two clinical isolates of 

Mycobacterium tuberculosis, MTB-489 and MTB-476, isolated from the Almaty region. These strains were part of a 

repository that was created during our project “Creating prerequisites of personalized approach in the diagnosis and 

treatment of tuberculosis, based on whole genome-sequencing of M. tuberculosis”. Two strains were isolated from 

sputum samples of patients P1 and P2. Phenotypically, two isolates were drug-susceptible M. tuberculosis. Sequence 

data was compared with the publicly available data on M. tuberculosis laboratory strain H37Rv and others. The 

sequencing of the strains was performed on a Roche 454 GS FLX+ next-generation sequencing platform using a 

standard protocol for a shotgun genome library. The whole genome sequencing was performed for two 

M.tuberculosis isolates MTB-476 and MTB-489. 96 M bp with an average read length of 520 bp, approximately 

21.8X coverage and 104.2 M bp with an average read length of 589 bp and approximately 23.7X coverage were 

generated for the MTB-476 and MTB-489, respectively. The genome of MTB-476 consists of 257 contigs, 4204 

CDS, 46 tRNAs and 3 rRNAs. MTB-489 has 187 contigs, 4183 CDS, 45 tRNAs and 3rRNAs. 

  

Conclusion: The results of genome assembling have been submitted into NCBI GenBank and are available for 

public access under the accession numbers AZBA00000000 and AZAZ00000000. These genome assemblies can be 

useful for comparative genome analysis and for identification of novel SNPs and gene variants in genomes of 

M.tuberculosis.  

Keywords: tuberculosis, genome sequencing, Kazakhstan 

 

 

 

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