Herbal extracts in the treatment of Diabetic Foot Syndrome


 

 

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Herbal extracts in the treatment 

of Diabetic Foot Syndrome 

 

Tatyana Kustova1, Tatyana 

Karpenyuk1, Alla Goncharova1, 

Leonid Mamonov2, Samir Ross3 

 
1Department of Biology and 

Biotechnology, Al-Farabi Kazakh National 

University, Almaty, Kazakhstan; 
2Institute of Plant Biology and 

Biotechnology; 3School of Pharmacy, 

University of Missisippi, Oxford, 

Mississippi 

 

 

Vol. 2, Suppl. (2013)   |   ISSN 2166-7403 (online)    

DOI 10.5195/cajgh.2013.86   |   http://cajgh.pitt.edu 

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KUSTOVA 

 

 

This work is licensed under a Creative Commons Attribution 3.0 United States License. 

 

This journal is published by the University Library System of the University of Pittsburgh as part  

of its D-Scribe Digital Publishing Program and is cosponsored by the University of Pittsburgh Press. 

 

Central Asian Journal of Global Health 

Volume 2, Suppl. (2013)  |  ISSN 2166-7403 (online)  |  DOI 10.5195/cajgh.2013.86  |  http://cajgh.pitt.edu 

  

 

Abstract 

Introduction: One of the most serious complications of diabetes is the formation of Diabetic Foot Syndrome. Herbal extracts 

that combine high antioxidant and antimicrobial properties can be used to treat the resulting neuropathy.    

The aim of this study was to determine antimicrobial and antioxidant activities of crude extracts isolated from plants growing in 

Kazakhstan, which could be used to develop products for treatment of Diabetic Foot Syndrome. 

Method: Different solvents, including dichloromethane and ethanol, were used to prepare plant extracts. The crude extracts from 

the plants were tested for antimicrobial activity using a modified version of the CLSI/NCCLS methods. All organisms were 

obtained from American Type Culture Collection. These included the fungi Candida glabrata ATTC 90030, the bacteria 

Staphylococcus aureus ATCC 29213, and Methicillin-resistant S. aureus ATCC 43300. The 2,2-diphеnyl-1-picrylhydrazyl 

(DPPH) assay, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay were 

used to analyzed the antioxidant capacity. 

Results: The results clearly indicate that antibacterial and antifungal activities vary with plant species. Dichloromethane extracts 

produced favorable results in all assays. Epilobium hirsutum, Rhodiola quadrifida, Rumex confertus showed antifungal activity 

against Candida glabrata in all extracts where IC50 less than 3 μg/ml. Rumex confertus, Glycyrrhiza Uralensis and Vexibia 

alopecuroides showed anti-fungal activity against Staphylococcus aureus (IC50 =10.80 μg/ml), (IC50 =11.10 μg/ml), (IC50 =3.05 

μg/ml) and Methicillin-resistant S. aureus (IC50 =16.20 μg/ml), (IC50 =11.00 μg/ml), (IC50 =2.90 μg/ml) respectively.  In spite of 

this, Vexibia alopecuroides extract showed no antioxidant activity. The other extracts showed a dose dependent ABTS 

scavenging activity. IC50 values were for the following: 6.6 μg/ml Epilobium hirsutum; 4.5 μg/ml Rumex confertus; 3.8 μg/ml 

Rhodiola quadrifida, 5.7 μg/ml Glycyrrhiza Uralensis. Extracts of Epilobium hirsutum and Rumex confertus had high antioxidant 

activity greater than 85% inhibition of DPPH (P ≤ 0.05). 

Conclusion: The demonstrated antimicrobial and antioxidant activities showed evidence supporting the use of herbal extracts to 

treat Diabetic Foot Syndrome.  

Keywords: diabetes, diabetic food syndrome, herbal extracts, Kazakhstan 

 

 

 

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