id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
can-1685	Echeverry-Chica, Julián; Naranjo-Díaz, Andrea; Araque-Marín, Pedronel	Silver nanoparticles functionalized in situ with D-limonene: Effect on antibacterial activity	2022	12	.pdf	application/pdf	6835	317	44	(bilateral) Difference in means AgNP + limonene 0.000 5 1.000 0.0 AgNP only 1.571 5 0.177 3.0 Limonene only 4.999 5 0.004 14.0 Disinfectant based on hydrogen peroxide 5.609 5 0.002 26.7 Quaternary ammonium-based disinfectant −1.532 5 0.186 −4.3 Having the behavior of the average concentra- tions necessary to inhibit the growth of the differ- ent bacteria, Student’s t-test was performed taking as reference the mean given for formulation F1 (AgNP and d-limonene) 26.3 μg∙mL−1. The formulation named F1 showed an outstand- ing broad-spectrum antimicrobial activity since it acted similarly on Gram-positive cocci and Gram-negative bacilli, eliminating the in vitro growth of all the bacteria tested with a MIC and 43 BMC of 28 μg∙mL−1 with an average of 26.3 μg∙mL−1; the formulation named F2 presented an MIC and a WBC of 34 μg∙mL−1 and formulation F3 showed an MIC and a WBC of 50 μg∙mL−1; these data indicate that AgNPs functionalized in situ with d-limonene in a nanoemulsion type formulation present an antimicrobial additive effect, being nec- essary less quantity of formulation F1 to eliminate 99.9% of the tested bacteria, compared to F2 and F3.	cache/can-1685.pdf	txt/can-1685.txt