DOI: 10.3303/CET2293025 Paper Received: 5 December 2021; Revised: 15 March 2022; Accepted: 8 May 2022 Please cite this article as: Medina Mori M., Suarez Alvites H., del Pilar Lopez Padilla R., Castaneda-Olivera C.A., Benites Alfaro E.G., 2022, Alternative Energy by Bioelectrogenesis from the Bacteria Pseudomonas Aeruginosa and Aeromonas Hydrophila, Chemical Engineering Transactions, 93, 145-150 DOI:10.3303/CET2293025 CHEMICAL ENGINEERING TRANSACTIONS VOL. 93, 2022 A publication of The Italian Association of Chemical Engineering Online at www.cetjournal.it Guest Editors: Marco Bravi, Alberto Brucato, Antonio Marzocchella Copyright © 2022, AIDIC Servizi S.r.l. ISBN 978-88-95608-91-4; ISSN 2283-9216 Alternative Energy by Bioelectrogenesis from the Bacteria Pseudomonas Aeruginosa and Aeromonas Hydrophila Mariela Medina Mori, Haydeé Suarez Alvites, Rosario del Pilar López Padilla, Carlos Castañeda Olivera, Elmer Benites-Alfaro* Universidad César Vallejo, Av. Alfredo Mendiola 6232 Los Olivos Lima Perú ebenitesa@ucv.edu.pe Bioelectrogenesis allows the transformation of chemical energy into electrical energy by means of microbial fuel cells.The research aimed to determine the amount of energy generated by bioelectrogenesis using Pseudomonas aeruginosa and Aeromonas hydrophila bacteria. Four double chamber H-type glass microbial fuel cells with a capacity of 500 mL and 2 control cells were constructed, using graphite or aluminium rod electrodes at the anode and graphite rod at the cathode for all the cells. 325 mL of anaerobic sludge and 75 mL of wastewater from a wastewater treatment plant were inoculated as substrate for the bacteria in the anode, where 50 mL of the aforementioned bacteria strain were inoculated, respectively. The experimental part was carried out in 20 days; the conditions of the cells were evaluated in terms of temperature and pH, characteristics of the bacteria and the behaviour of the voltage generated. It was established that the bacteria that generated the highest voltage was Pseudomonas aeruginosa with 0.8960V, using an aluminum electrode in the anode chamber. The results indicate that bioelectrogenesis using bacteria in anaerobic sludge and wastewater is a promising technology for obtaining clean and low-cost energy. 1. Introduction Fossil energy sources in the world are becoming increasingly scarce, so there is a need to replace them with sustainable and renewable energy, one of the alternatives is bioenergy, which according to data only represents 10% of primary energy worldwide (IEA, 2022). An emerging biotechnology application is the generation of energy using microbial fuel cells where microorganisms are used to convert the chemical energy in a substrate into electrical energy through metabolic activity by transferring electrons to an anode electrode which, when placed in circuit with a cathode chamber separated by a proton membrane, produces electrical energy (Revelo et al., 2013). Research has been carried out on the use of bioelectrogenesis, it is indicated that the substrate has a relevant role in the anodic behavior of the microbiological fuel cell, it has been tested with acetate as a substrate in the cells, giving a significant generation of electricity; In the same way, the characterization of the microorganisms in suspension and the biofilm by means of the Illumina technique, showed that Desulfuromonas, Solitalea, Acholeplasma, Desulfobacula and Sphaerochaeta are the main responsible for the generation of energy (Mateo S., 2018). Electrical energy has been obtained from wastewater from the Rio Seco industrial park, with the syntrophic association of the microalgae Chlorella vulgaris and Scenedesmus obliquus and a consortium of anaerobic bacteria native to the wastewater, this bioelectrochemical system generated an average voltage of 66. 50 ± 1.70 mV, an average current density of 0.02 ± 0.00 mA/mm2 and an average power density of 2.43 ± 0.33 mW/mm2, up to 14 days of evaluation (Terán, 2017). The design of a microbial fuel cell with electrodes such as copper and zinc, allowed the generation of bioelectricity whose average circuit voltage was 0.507 V, current of 476.7 μA and maximum power density of 0.24 mW/cm2; Also, the conductivity in the anode chamber was around 14.5 to 7.75 μS/cm with an average pH of 8.0. (Rojas, S. et al., 2019). 145 The selection of materials, measurements and components of a fuel cell have a positive impact on energy generation; in a microbial fuel cell process with temperatures ranging from 45 °C to 28 °C for 30 hours of evaluation, the optimum temperature was 38.31 °C using Pseudomonas aeruginosa bacteria and 37.9 7°C for Escherichia coli; In addition, pH values of 6. 3 and 6.5 with Escherichia coli bacteria generate higher energy, while using Pseudomonas aeruginosa the values should be close to 7. The average voltage generated by Pseudomonas aeruginosa was 294.17 mV, while for Escherichia coli produced an average voltage of 186.44 mV (Bermúdez and Bernal, 2018). 2. Methodology The research was experimental in nature and was carried out according to the following steps: 2.1 Obtaining the bacteria and adaptation. Bacterial strains of Pseudomonas aeruginosa and Aeromonas hydrophila species were seeded to verify their adaptability and reproduction using Mc Hillton and Mac Conkey agar culture media, respectively. 2.2 Obtaining and characterization of the substrate The substrate was obtained consisting of anaerobic sludge with water from the CITRAR-UNI domestic wastewater treatment plant. These sludge and water were characterized for their physicochemical properties at the César Vallejo University Laboratory before and after being used as substrate in the microbial fuel cell process, see Table 1. Table 1: Characteristics of the sludge, initial and in the treatment cells Parameter Initial Cell 1: Aeromonas hydrophila bacteria Cell 2: Pseudomonas aeruginosa bacteria Cell 3: Aeromonas hydrophila bacteria Cell 4: Pseudomonas aeruginosa bacteria Standard deviation pH 8.0 8.05 7.90 8.04 8.15 0.10 Temperature (°C) 22.3 22.4 22.5 22.7 22.7 0.15 Conductivity ((μS/cm) 3.10 13.80 8.40 7.8 3.70 4.14 Turbidity (NTU) 77.0 50.0 61.0 33.6 35.1 13.02 Dissolved oxygen (ppm) 5.19 0.60 8.34 5.59 5.56 3.22 2.3 Microbial Cell Design and Construction Six 500-mL double-chamber H-type glass microbial cells joined by a 10-cm glass bridge were designed and constructed, as shown in Figure 1. A graphite electrode was used as cathode, a graphite electrode or aluminum as anode (depending on the assay) and proton exchange membrane bridge with agar-agar solution (a 100 mL syringe was used). The experimental design and the description of the cells for the investigation is presented in Table 2. Figura 1: Microbial cell 146 Table 2: Description of the anodic and cathodic cells for each design Cell 1 design Cell 2 design Cell 3 design Cell 4 design Cell 5 Design (Control) Cell 6 Design (Control) Anodic Cell Bacteria: 50 mL of inoculum of Aeromonas hydrophila 50 mL of inoculum of Aeromonas hydrophila 50 mL of inoculum of Pseudomonas aeruginosa 50 mL of inoculum of Pseudomonas aeruginosa - - Electrode type: Graphite (44.37 cm2) Aluminum (36 cm2) Graphite (44.37 cm2) Aluminum (36 cm2) Graphite (44.37 cm2) Aluminum (36 cm2) Sustrato: Sludge: 235 mL Residual water: 75 mL Sludge: 235 mL Residual water: 75 mL Sludge: 235 mL Residual water: 75 mL Sludge: 235 mL Residual water: 75 mL Sludge: 235 mL Residual water: 75 mL Sludge: 235 mL Residual water: 75 mL Cathodic Cell Electrode type: Graphite (22 cm2) Graphite (22 cm2) Graphite (22 cm2) Graphite (22 cm2) Graphite (22 cm2) Graphite (22 cm2) Medio: Copper sulfate solution (1 M): 400 mL Copper sulfate solution (1 M): 400 mL Copper sulfate solution (1 M): 400 mL Copper sulfate solution (1 M): 400 mL 400 mL of distilled water 400 mL of distilled water 2.4 Power generation process In the process of energy generation with the cells with the different designs, it was monitored for 20 days, 64 measurements of the voltage generated were made; in the first 8 days it was measured twice a day every 12 hours and in the following days, 4 times a day every 6 hours. The pH, temperature and bacterial population of the anodes of the experimental cells were also recorded. 3. Results and discussion 3.1 Cell temperature during the process The cells maintained a temperature between 20.8 °C and 21.8 °C, those in the anode were inoculated with Aeromonas hydrophila and Pseudomonas aeruginosa bacteria, as well as in the control cells. (Figure 2); these temperatures were relatively low and could affect the optimal generation of energy because scientific literature indicates that taking into account that the presence of substrates and their concentration requires more time to obtain a constant voltage of electric current in a microbial cell, the appropriate temperature for the generation of electric energy and removal of organic matter in the wastewater is 25°C reaching high columbic efficiencies between 28. 4% and 70.69%, for chemical demands of 1980 mg/L and 3200 mg/L; therefore, the oxidation process in the anode substrate, is directly related to the temperature, in the generation of electric power (Valencia, 2018). Figure 2: Cell temperatures 19.5 20 20.5 21 21.5 22 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 T e m p e re a tu ra ( °C ) Time (Days) Temperature (c1) Temperature (C2) Temperature (C3) Temperature (C4) Temperature (C5) Temperature (C6) 147 3.2 Energy generated in Microbial Cell 1 (C1) This cell consisted of an anode cell where Aeromonas hydrophila bacteria were inoculated in a sludge medium with wastewater and a graphite electrode was placed, according to the Cell 1 design mentioned in Table 2. As shown in Figure 3, in this cell the energy generation was increasing with a logarithmic trend as time progressed, possibly due to the growth of the bacterial population and was higher than the energy generated in the control cell where no bacteria were inoculated (Cell 5), by 6.31 % on day 20. Figure 3: Energy generated in microbial cell 1 3.3 Energy generated in Microbial Cell 2 (C2) This cell corresponded to cell design 2 (see Table 2), it consisted of an anodic cell where Pseudomonas aeruginosa bacteria were inoculated in a sludge medium with wastewater and an aluminum electrode was placed. The energy generated in this cell on day 5 was 0.4484 V, higher than the energy generated by the control cell 5 (control), from day 6 the energy generated had a very slow growth so that the energy generated in the control cell (without bacteria) was higher (Figure 4). So, in this cell the inoculated bacteria did not improve the generation of energy in the way that, if it happened with the other microbial cells, it is very likely that this result was influenced by the temperature that in some cases inhibits the generation of energy (Valencia, 2018). Figure 4: Energy generated in Microbial Cell 2 Comparing Cell 1 with Aeromonas hydrophila bacteria and Cell 2 with Pseudomonas aeruginosa bacteria, where in both anode chambers graphite electrode was used, it was in cell 2 that the energy generated was lower. In cell 2 the pH was in the range of 7.2 to 7.9 and presented optimal conditions for population growth of the bacteria that was higher than cell 1 and the others; it is important to keep in mind this parameter because it can affect the solubilization of organic compounds (such as phosphates) for the performance of microorganisms (Acosta- Suárez et al., 2019). 8.1 8.0 0.8240 0.7720 0.0000 0.1000 0.2000 0.3000 0.4000 0.5000 0.6000 0.7000 0.8000 0.9000 7.2 7.4 7.6 7.8 8 8.2 8.4 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 V o lt a g e ( V ) p H Time ( days) pH Energy (C1) Energy (C. control 5) 7.9 7.7 0.4990 0.7990 -0.1000 0.1000 0.3000 0.5000 0.7000 0.9000 7.1 7.3 7.5 7.7 7.9 8.1 8.3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 V o lt a g e ( V ) p H Time (days) pH Energy (C2) Energy (C. control 5) 148 3.4 Energy generated in Microbial Cell 3 (C3) Cell 3 with an anode chamber where Aeromonas hydrophila bacteria were inoculated in a sludge medium with wastewater and an aluminum electrode was placed. The energy generated from day 7 when it reached 0.8366 V was almost similar until day 19 when it was 0.8410 V, see Figure 5. The energy generated by this cell, in the same time interval, was higher than that generated in the control cell by approximately 10 %. Figure 5: Energy generated in Microbial Cell 3 3.5 Energy generated in Microbial Cell 4 (C4) In Cell 4 with an anode cell inoculated with Pseudomonas aeruginosa bacteria in a sludge medium with wastewater and an aluminum electrode was placed. The energy obtained in cell 4 from day 2 was higher than the energy generated in the control cell until day 20 of monitoring, reaching the highest level on day 18 with the value of 0.8960 V while in the control cell it was 0.7530 V, i.e., with a margin of 15.95 % higher, see Figure 6. The pH was progressively increasing in the interval from 7.3 to 8.44. Figure 6: Energy generated in Microbial Cell 4 It was determined that using the same aluminum electrode in the anodic cell and the cathodic cell with graphite electrode, when Pseudomonas aeruginosa was inoculated, the microbial cell produced more energy than when Aeromonas hydrophila bacteria were inoculated. Of the 4 microbial cells, it resulted that cell 4 generated more energy with 0.8960 V, then cell 3 with 0.8410 V, cell C1 with 0.8240 V and finally cell C2 with 0. 4920 V; that is when using in the anionic cell Pseudomonas aeruginosa bacteria in sludge substrate with wastewater, with aluminum electrode and in the cathodic cell a graphene electrode in copper sulphate solution, proved to be the most optimal in obtaining energy; therefore, microbial fuel cells allows obtaining bioelectricity while in this process decreases organic pollutants with the presence of carbon and nitrogen (Sawasdee V. and Pisutpaisal N., 2018) that in high levels are found in sewage 0.8410 0.7510 0.0000 0.2000 0.4000 0.6000 0.8000 1.0000 6.9 7.1 7.3 7.5 7.7 7.9 8.1 8.3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 V o lt a g e ( v)p H Time (Days) pH Energy (C3) Energy (C. control 6) 0.8960 0.7570 0.7530 0.0000 0.2000 0.4000 0.6000 0.8000 1.0000 7.2 7.3 7.4 7.5 7.6 7.7 7.8 7.9 8 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 V o lt a g e ( v) p H Time (Days) pH Energy (C4) Energy (C. control 6) 149 sludge or wastewater treatment plants and require convenient handling such as also use of fixed bed gasifiers to produce hydrogen (Zaccariello and Mastellone, 2020). 3.6 Bacterial growth in anionic cells The bacteria inoculated in the anionic chambers of the microbial cells had the population growth shown in Table 3, where it was found that bacteria cell C2 reached day 20 with a higher population than the other cells. This bacterium, due to its great capacity for adaptability and metabolizing various types of substrates, allows its growth, as graphite does not interfere with the transfer of protons through the selective membrane, favouring microbial activity. Table 3: Bacterial growth in the cells Time (Days) Cell 1: Number of Aeromonas hydrophila bacteria Cell 2: Number of Pseudomonas aeruginosa bacteria Cell 3: Number of Aeromonas hydrophila bacteria Cell 4: Number of Pseudomonas aeruginosa bacteria 0 0.019 0.019 0.019 0.019 4 1.44E+10 3.42E+10 1.52E+10 8.55E+09 8 8.59E+10 1.216E+11 4.8336E+11 1.52E+11 20 5.244E+11 6.3232E+11 3.61E+09 9.12E+10 4. Conclusion The generation of energy in microbial fuel cells is a feasible alternative to be used and perfected to generate sustainable renewable energy, so it requires further research into the conditions conducive to making this method of bioelectrogenesis efficient; this research verifies this possibility, finding that Pseudomonas aeruginosa bacteria with sludge support with wastewater and aluminum electrode in the anode chamber generated 0.8960 V, higher than in cells where Aeromonas hydrophila and graphite electrodes were used. Acknowledgments To Universidad César Vallejo for their support in the dissemination of this research. References Acosta-Suárez M., Cruz-Martín M., Pichardo T., Rodríguez, E., Barbón R., Capote A., Pérez, A., Alvarado-Capó Y., 2019. Solubilización de fosfatos in vitro por cepas de Aspergillus y Penicillium y promoción del crecimiento de plantas de cafeto. Biotecnología Vegetal 19, 65–72. Bermúdez M. y Bernal E., 2018. 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