CHEMICAL ENGINEERING TRANSACTIONS VOL. 56, 2017 A publication of The Italian Association of Chemical Engineering Online at www.aidic.it/cet Guest Editors: Jiří Jaromír Klemeš, Peng Yen Liew, Wai Shin Ho, Jeng Shiun Lim Copyright © 2017, AIDIC Servizi S.r.l., ISBN 978-88-95608-47-1; ISSN 2283-9216 Feedstock Amendment for the Production of Quality Compost for Soil Amendment and Heavy Metal Immobilisation Lim Li Yeea,b , Lee Chew Tin*a,b , Lim Jeng Shiunb,c, Jiří Jaromír Klemešd, Ho Chin Siongb,e, Nur Naha Abu Mansorf a Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia (UTM), 81310 UTM Skudai, Johor Bahru, Johor, Malaysia b UTM Low Carbon Asia Research Centre, Universiti Teknologi Malaysia (UTM), 81310 UTM Skudai, Johor Bahru, Johor, Malaysia c Process Systems Engineering Centre (PROSPECT), Research Institute for Sustainable Environment, Universiti Teknologi Malaysia (UTM), 81310 UTM Johor Bahru, Johor, Malaysia d Faculty of Information Technology and Bionics (Pázmány Péter Katolikus Egyetem, Információs Technológiai és Bionikai Kar), Práter u. 50/a, 1083 Budapest, Hungary e Faculty of Built Environment, Universiti Teknologi Malaysia (UTM), 81310 UTM Johor Bahru, Johor, Malaysia f Faculty of Management, Universiti Teknologi Malaysia (UTM), 81310 UTM Johor Bahru, Johor, Malaysia. ctlee@utm.my Composting is a waste management technology for recycling organic waste. A compost without detectable of pathogens and heavy metals can act as a soil conditioner or organic fertilizer to promote plant growth. It provides the soil with organic matter and nutrients, improving soil texture and water holding capacity and suppressing plant diseases with the presence of beneficial microorganisms. Many researchers aim to improve the quality of compost at a reduced process cost and composting duration. Preferably the composting process also offers the co-benefits of reduced environmental impact. The quality of the compost is closely related to the feedstock used for the composting. The feedstock ranges from bio-waste, plant- and animal-based agricultural waste and sometimes the organic fraction in the municipal solid waste. This paper reviews a range of feedstock amendment methods including the addition of bulking agent or chemical additive, inoculation with earthworm or microbial inoculants, and the addition of the mature compost, that could influence the composting process and the end product quality. The potential of immobilisation of the heavy metals in the waste material through composting was also discussed. 1. Introduction As the backbone of many developing countries, agricultural sectors face various global challenges, including climate change, urbanization, and some environmental issues such as leaching and accumulation of pesticides and fertilizers. The performance of the conventional farming associated with the overconsumption of chemical fertilizers has led to some severe environmental problems. To cope with the natural leaching caused by the loss of around 2/3 of the applied fertilizers, high amount of fertilizers has been applied and resulted in the contamination of ground water (especially by nitrate, or some other pollutants such as pesticides) (Devassine et al., 2002). World fertiliser consumption rate had increased from 98.7 to 120 kg per hectare of arable land from year 2002 to 2013 (Food and Agriculture Organisation, 2016). According to the Department of Statistics (2016), Malaysia’s population reaches 31.7 million in 2016 with an annual growth rate of 1.5 % where 71 % of the total population has resided in the urban area in tandem with the rapid development of Malaysia. Rapid population growth and urbanisation have increased the generation of the solid waste. In many fast developing countries such as Malaysia, landfilling remained the main disposal method, i.e about 94.5 % of the collected wastes is disposed in the landfills, with a recycling and composting rate of 5.5 and 1 % (Periathamby et al., 2009). About 57 % of the waste is made out of organic solid waste (Saeed et al., 2009). DOI: 10.3303/CET1756084 Please cite this article as: Lim L.Y., Lee C.T., Lim J.S., Klemeš J.J., Ho C.S., Mansor N.N.A., 2017, Feedstock amendment for the production of quality compost for soil amendment and heavy metal immobilisation, Chemical Engineering Transactions, 56, 499-504 DOI:10.3303/CET1756084 499 Composting can be an alternative to overcome these challenges. Composting is a biological process which converts organic wastes into a stable organic product with high humic substances that last longer in soil. It involves a relatively low processing cost and less complicated technology. Composting has been widely practised in many developing countries however the replacement ratio of compost for chemical fertilizer is still low due to the inconsistency in the end quality of compost (Fan et al., 2016). Composting transforms the organic wastes into compost that can be used to improve soil quality, minimise soil erosion, and promote plant growth. Table 1 lists general requirements for organic fertiliser according to Malaysia Standard MS1517:2012. This paper reviews the feedstock amendment strategies during composting to enhance the end compost quality and the immobilisation of bioavailable heavy metal in compost and soil. Table 1: General requirements for organic fertiliser Characteristic Recommendation Range Moisture Content ≤ 30 % (wet weight basis) Organic Matter Content ≥ 50 % Nitrogen Content ≥ 1.5 % C/N Ratio ≤ 25 : 1 Maximum Permissible Value for Heavy Metals Cr ≤ 200 mg/kg; Pb ≤ 300 mg/kg; Ni ≤ 150 mg/kg; Cd ≤ 5 mg/kg; Hg ≤ 2 mg/kg; As ≤ 50 mg/kg Pathogenic Evaluation E. coli < 10 cfu/g; Pseudomonas aeruginosa < 10 cfu/g; Salmonella: Absent; Staphylococcus aureus < 10 cfu/g 2. Feedstock amendments during composting Several amendments can be made during the composting process to improve the efficiency of the process as well as the quality of the final compost. A range of amendment strategies during composting has been reported to mitigate the emission of greenhouse gases (GHG) (Bong et al., 2016). Common amendment strategies include the control of the aeration rate, addition of bulking agent or chemical additive, inoculation with earthworm or microbial inoculants, and covered or mixed the compost with the mature compost. Table 2 shows the positive effect of different amendments in the final compost quality. Most of the amendments gave more than 50 % of increment in the final nutrient concentrations with lower carbon-to-nitrogen (C/N) ratios indicating a better degree of waste degradation. For instance, a two-stage composting technology, amended with earthworm and chemical (Zhang and Sun, 2015) or matured compost (Zhang and Sun, 2016), has enhanced the decomposition of the recalcitrant green waste. It was likely that the beneficiary microbes in the mature compost improved the biochemical transformation of the organic materials, while earthworms enhanced the substrate availability to microbes (Lim et al., 2014). This innovative composting system has shortened the traditional composting period (90 - 270 d) to 30 d. The addition of microbial inoculant (MI) improves the degradation of the organic waste and humic substance formation. It is a common practice when interact with those recalcitrant organic wastes, i.e. lignocellulosic- and cellulosic- based wastes such as oil palm empty fruit bunches and wood bark. Previous studies indicated that the effect of MI for composting is more significant on the recalcitrant organic wastes than the easily degradable waste such as the food waste. MI enhances the initial microbial population in the waste by providing higher concentration of enzymes for the degradation of substrates. Single MI tends to offer a more specific and reproducible effect when degrading specific substrates. A similar quality of final compost was obtained when the sugar-cane waste by-products were inoculated with either Pleurotus sajorcaju alone or the mixed culture of Pleurotus sajorcaju, Trichoderma viridae, Aspergillus niger, and Pseudomonas striatum (Kumar et al., 2010). The effect of single or mixed MI is complicated and specific to the characteristics of the feedstock and operating conditions.The addition of chemical or mineral additive, such as zeolite and biochar, are able to increase the water and nutrient retention, reduce N volatilisation, improve aeration, enhance microbial activities, mitigate GHG emission, and immobilise the heavy metal in compost and soil following compost application (Zhang and Sun, 2015). According to Zhang and Sun (2015), zeolite have the unique structure that is efficient in adsorbing gases, water, nutrients, and heavy metals and stabilising the final compost. Covering or mixing the compost pile with the mature compost was report to reduce the GHG emission, the mature compost can also serve as the microbial inoculant for the next cycle of composting (Fukumoto et al., 2011). Although aeration rate is the main factor that influences the compost stability and GHG emission, it has little impact in the final compost quality (Yuan et al., 2016). 500 Table 2: Type of amendment made during composting and their respective effect in improving the final compost quality Refere nce Study Area Composting System Waste Duration (d) Amendment Nutrient content (%) C/N N P K Zhang and Sun (2015) Beijing, China Two-stage composting process: • Primary Fermentation (PF): cement containers (6 L × 2 W × 1.5 H m) with automated turning and watering system • Second Fermentation (SF): windrow with trapezoidal cross- section (2 L × 1.5 W × 1 H m) Green waste PF: 6 SF: 24 a) No amendment 17.22 2.27 0.12 0.30 b) 0.3 % earthworm casts + 25 % clinoptilolite 5.02 4.23 0.58 0.91 Zhang and Sun (2016) Beijing, China Two-stage composting process: • PF: cement containers (6 L × 2 W × 1.5 H m) with automated turning and watering system • SF: windrow with trapezoidal cross- section (2 L × 1.5 W × 1 H m) Green waste PF: 6 SF: 24 a) No amendment -- 2.07 0.08 0.26 b) 15 % woodchip + 35 % composted green waste -- 4.90 0.72 1.01 Lim et al. (2014) Selangor, Malaysia Rectangular plastic containers (17 cm × 14 cm × 12 cm) Compost was periodically turned for 2 weeks followed by a 6- weeks vermicomposting POME 56 a) 1 part rice straw : 3 parts POME (without earthworms) 20.36 -- 0.36 1.01 b) 1 part rice straw : 3 parts POME (with earthworms) 9.64 -- 0.63 2.14 Kumar et al. (2010) India 60 kg pre-decomposed substrates (6 d) were subjected to compost (30 d) with microbial inoculation followed by vermicomposting (another 40 d) Press- mud: bagasse: sugarcane trash = 1: 1: 1 70 a) Without microbial inoculant -- 0.96 0.88 0.60 b) With microbial inoculant (P. sajorcaju) -- 1.40 1.44 1.82 Awasthi et al. (2016) Shanxi, China 130 L bench-scale PVC composter air was pumped from the bottom into the composter with a constant air flow about 0.35 L h −1 kg−1 dry weight during thermophilic phase dewatered fresh sewage sludge: wheat straw = 1: 1 (C/N ~25) 56 a) No amendment 25.7 1.63 0.93 2.85 b) Amended with 1 % lime and 12% biochar 11.8 2.41 1.24 2.70 501 According to Yuan et al. (2016), higher AR (≥ 0.3 L·kg-1 dry matter·min-1) might lead to the insufficient in compost sanitation, especially when running a laboratory scale composting. The insufficient heat during the process caused by high AR also decreased the degree of compost maturity and humification. Low (0.4 L·kg- 1·min-1) to medium (0.6 L·kg-1·min-1) AR were suggested by Rasapoor et al. (2009) when running a larger scale (3 m wide × 6 m long × 1.6 m high) composting process when considering from the aspect of energy consumption and overall compost maturity. Table 3: Effect of composting or compost for the immobilisation of heavy metal and compost quality Refere nce Study Area Type of Compost Nutrient content (%) Experimental Design Composting Effect C/N HA pH Al Mamun et al. (2016) New Zealand Municipal compost 9.10 -- 7.40 Addition of 2.5% compost to soil (Pukekohe and Levin soil) Application of compost decreased the Cd uptake by 20 – 60 % (plant type: onion, spinach, lettuce) Tapia et al. (2010) Madrid, Spain Pruning waste + biosolids (1 : 3 w/w) compost 15.20 8.40 6.90 Sequential extraction of Cd from compost after 4-week incubation with 80 or 200 mg/kg Cd Only 0.2 % of Cd leached out from the compost Lv et al. (2016) Shang- hai, China Vermicompo sted cattle dung (CD) 13.60 6.23 7.90 Using sequential extraction to evaluate the effects of vermicomposting on the Speciation and mobility of heavy metals (Zn, Pb, Cr, and Cu) in CD and PM The total heavy metals in the final CD and PM were higher than the initial values and also the control without the worms (due to the concentrated of substrates), however vermicomposting decreased the migration and availability of heavy metals Vermicompo sted pig manure (PM) 13.30 5.70 7.10 Song et al. (2014) Su- zhou, China Vermicompo sted cow manure/ mushroom residues 11.32 5.62 7.57 Investigating the effects of vermicomposting on the mobility and availability of heavy metal Composting increased the total heavy metal concentration irrespective of the presence of earthworm in both cases; but the availability of heavy metal significantly decreased especially in vermicomposting Vermicompo sted pig manure/ mushroom residues 10.43 6.70 7.35 Awasthi et al. (2016) Shaan xi, China Dewatered fresh sewage sludge : wheat straw 11.80 17.23 7.72 Evaluating the effect of lime and biochar amendment on bio-availability of heavy metals Amended compost showed higher humic acid (17.23 %) concentration which reduced the bio-availability of heavy metals (34.81 % Cu, 56.74 % Zn, 87.96 % Pb and 86.65 % Ni) and improved compost maturity Singh and Kalamd had (2013) India Water hyacinth: cattle manure: sawdust = 6 : 3 : 1 -- -- 7.3- 7.7 Determining the bioavailability and leachability of heavy metals (Zn, Cu, Mn, Fe, Ni, Pb, Cd and Cr) in compost mixed with lime Addition of lime (≤2 %) can enhance the composting temperature (to >57 °C), organic matter degradation (up to 38.5 %) and reduce the bioavailability and leachability of heavy metals (>61 %) 502 3. Immobilisation of heavy metal by compost Intensification of industrialisation and urbanisation, and the intensive use of chemical fertiliser, herbicide, pesticide, and fungicide, have caused the contamination of many agricultural soils with heavy metals (Khairiah et al., 2012). These heavy metals can lead to severe health issue if they enter the food chain via the vegetables and animal feedstock. Composting can be a potential solution to overcome this issue. Composting has been reported to immobilise the heavy metals in the waste. Table 3 shows the effect of composting on the immobilisation of heavy metal in the contaminated waste and soil. With the formation of humic substances (HS), composting can reduce the bioavailability of heavy metals. HS are physically and chemically homogeneous mixture with relatively high molecular mass and stability. The present of carboxyl and hydroxyl groups in HS allowed their strong interaction with heavy metals. The concentration of heavy metals increased during the composting process due to the concentrated effect of substrates, however the bio-availability of the heavy metal was significantly decreased. Reduced bio-availability of the heavy metal is crucial to avoid the uptake by the plants. All reviews on the immobilisation of heavy metal by the amendment methods are summarised in Table 3. Apart of the presence of HS, pH is another key factor that affects the mobility of heavy metal. The higher the pH of the compost (pH 6 and above), the more stable the insoluble complexes formed between heavy metals and the organic matters (Tapia et al., 2010). According to Al Mamun et al. (2016), the uptake of Cd by plants has been reduced by 30 – 60 % at a higher pH of the soil (at pH 6.2) following the application of compost. The application of amendments, such as earthworm inoculation and alkaline materials and mineral additives, into compost also improved the immobilisation of heavy metal. The mineral additives can improve the heavy metals binding by the stable organic matter while the addition of alkaline material, such as lime, can increase the compost pH and these further lead to the decrease in the bioavailability of heavy metals (Singh and Kalamdhad, 2013). Vermicomposting combined the positive effects by the earthworms and microorganisms. Vermicomposting could offer a higher degree of humification (Lv et al., 2016), as it could directly or indirectly accelerate the humification process through their digestion, burrowing or simulating (Song et al., 2014). Earthworms can also bio-accumulate the heavy metal in their bodies. Vermicomposting seems to be more efficient in decomposing the waste with heavy metals contamination and to produce better quality compost with higher humus content and less phytotoxicity, and within a shorter period of composting. 4. Conclusions Composting can be a viable and effective treatment for organic waste management. With the application of feedstock amendments for composting, higher rates of composting, assimilation of nutrient contents and the immobilisation of heavy metals could be achieved. Future study should intensify the potential of microbial and earthworms to achieve high efficiency for the immobilisation of heavy metals content for the composting of organic waste followed by the plant uptake study on the bio-available of heavy metals. Acknowledgments The authors acknowledge research grants from the Ministry of Higher Education (MOHE) Malaysia with grant no. 7301.4B145 and 2546.15H25; and from Universiti Teknologi Malaysia with the grant no. 2546.14H65 and 2501.10H28. The authors also acknowledge the funding and support from JICA-JST-SATREPS entitled “Development of Low Carbon Scenarios in Asian Region” and support from the Faculty of Information Technology and Bionics, Pázmány Péter Catholic University in Budapest, Hungary. Reference Al Mamun S., Chanson G., Muliadi, Benyas E., Aktar M., Lehto N., McDowell R., Cavanagh J., Kellermann L., Clucas L., Robinson B., 2016, Municipal composts reduce the transfer of Cd from soil to vegetables, Environmental Pollution 213, 8-15. Awasthi M.K., Wang Q., Huang H., Li R., Shen F., Lahori A.H., Wang P., Guo D., Guo Z., Jiang S., Zhang Z., 2016, Effect of biochar amendment on greenhouse gas emission and bio-availability of heavy metals during sewage sludge co-composting, Journal of Cleaner Production 135, 829-835. Bong C.P.C., Lim L.Y., Ho W.S., Lim J.S., Klemeš J.J., Towprayoon S., Ho C.S., Lee C.T., 2016, A review on the global warming potential of cleaner composting and mitigation strategies, Journal of Cleaner Production, DOI: 10.1016/j.jclepro.2016.07.066. Department of Statistics Malaysia, 2016, Current population estimates, Malaysia, 2014 - 2016 <www.statistics.gov.my/index.php?r=column/cthemeByCat&cat=155&bul_id=OWlxdEVoYlJCS0hUZzJyRU cvZEYxZz09&menu_id=L0pheU43NWJwRWVSZklWdzQ4TlhUUT09> accessed 15.08.2016 503 Devassine M., Henry F., Guerin P., Briand X., 2002, Coating of fertilizers by degradable polymers, International Journal of Pharmaceutics 242(1–2), 399-404. Fan Y.V., Lee C.T., Klemeš J.J., Bong C.P.C., Ho W.S., 2016, Economic assessment system towards sustainable composting quality in the developing countries, Clean Technologies and Environmental Policy DOI: 10.1007/s10098-016-1209-9. Food and Agriculture Organization, 2016, Fertilizer consumption (kilograms per hectare of arable land) <data. worldbank.org/indicator/AG.CON.FERT.ZS?end=2013&start=2002&view=chart>accessed 20.09.2016 Fukumoto Y., Suzuki K., Kuroda K., Waki M., Yasuda T., 2011, Effects of struvite formation and nitratation promotion on nitrogenous emissions such as NH3, N2O and NO during swine manure composting, Bioresource Technology 102 (2), 1468-1474. Khairiah J., Tharmendren M.S.M., Habibah J., Zulkefly H., Kamal W.I.W., Ismail B.S., 2012, Heavy metal content in paddy soils of Ketara, Besut, Terengganu, Malaysia, World Applied Sciences Journal 19 (2), 183-191. Kumar R., Verma D., Singh B.L., Kumar U., Shweta, 2010, Composting of sugar-cane waste by-products through treatment with microorganisms and subsequent vermicomposting, Bioresource Technology 101 (17), 6707-6711. Lim S.L., Wu T.Y., Clarke C., 2014, Treatment and Biotransformation of Highly Polluted Agro-industrial Wastewater from a Palm Oil Mill into Vermicompost Using Earthworms, Journal of Agricultural and Food Chemistry 62 (3), 691-698. Lv B., Xing M., Yang J., 2016, Speciation and transformation of heavy metals during vermicomposting of animal manure, Bioresource Technology 209, 397-401. Periathamby A., Hamid F.S., Khidzir K., 2009, Evolution of solid waste management in Malaysia: impacts and implications of the solid waste bill, 2007, Journal of Material Cycles and Waste Management 11 (2), 96- 103. Rasapoor, M., Nasrabadi, T., Kamali, M., Hoveidi, H., 2009, The effects of aeration rate on generated compost quality, using aerated static pile method, Waste Management 29 (2), 570-573. Saeed M.O., Hassan M.N., Mujeebu M.A., 2009, Assessment of municipal solid waste generation and recyclable materials potential in Kuala Lumpur, Malaysia, Waste Management 29 (7), 2209-2213. Singh J., Kalamdhad A.S., 2013, Effects of lime on bioavailability and leachability of heavy metals during agitated pile composting of water hyacinth, Bioresource Technology 138, 148-155. Song X., Liu M., Wu D., Qi L., Ye C., Jiao J., Hu F., 2014, Heavy metal and nutrient changes during vermicomposting animal manure spiked with mushroom residues, Waste Management 34 (11), 1977-83. Tapia Y., Cala V., Eymar E., Frutos I., Gárate A., Masaguer A., 2010, Chemical characterization and evaluation of composts as organic amendments for immobilizing cadmium. Bioresource Technology, 101 (14), 5437-5443. Yuan J., Chadwick D., Zhang D., Li G., Chen S., Luo W., Du L., He S., Peng S., 2016, Effects of aeration rate on maturity and gaseous emissions during sewage sludge composting, Waste Management, DOI: 10.1016/j.wasman.2016.07.017. Zhang L., Sun X., 2015, Effects of earthworm casts and zeolite on the two-stage composting of green waste, Waste Management 39, 119-129. Zhang L., Sun X., 2016, Influence of bulking agents on physical, chemical, and microbiological properties during the two-stage composting of green waste, Waste Management 48, 115-126. 504