id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
cet-12518		cet-12518		6	.pdf	application/pdf	3130	111	44	The monitoring of microbial growth and its physiology allow to infer that the cells did not present stress when changing from an orbital shaking agitation to a continued stirred reactor, this system may favour oxygen mixing and transfer processes which may influence cell integrity 122 and increase in protein production (Bagagli and Sato 2013). Table 2: Protein production at Flask and Bioreactor level 1Average Production Enzyme; 2Percentage Improvement Production compared whit flask level 0,0 0,2 0,4 0,6 0,8 1,0 1,2 1,4 1,6 1 2 3 4 5 6 7 8 9 O pt ic al D en si ty 5 95 (n m ) Time (h) Biomass Production at 350 rpm Test 1 Test 2 0,0 0,2 0,4 0,6 0,8 1,0 1,2 1,4 1 2 3 4 5 6 7 8 9 O pt ic al D en si ty 5 95 (n m ) Time (h) Biomass Production at 220 rpm Test 1 Test 2 a) b) Agitation (rpm) Test NIC1 (µg/mL) ANIC2 (µg/mL) IC3 (µg/mL) AIC4 (µg/mL) 220 1 857 883.25 1352.66 1465.83 2 909.5 1579 350 1 1141 1197.75 3071 2973.5 2 1254.5 2876 Scale Agitation (rpm) APE1 (µg/mL) PIP2 (µg/mL)	cache/cet-12518.pdf	txt/cet-12518.txt