id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
cet-6583		cet-6583		6	.pdf	application/pdf	3803	157	51	In the case of acid urease immobilised onto Eupergit® C250L in the same range of urea concentrations, the ammonia formation rate (r’A) for immobilised acid urease referred to liquid volume was reconstructed by using the Michaelis-Menten kinetic model too: LMa L ' max' A SK S V r + = ( 4) with V’max = k’cat cPi ( 5) cPi = cBd YP/B ( 6) where V’max is the maximum ammonia formation kinetic rate constant for immobilised enzyme, KMa or k’cat the apparent Michaelis constant or specific ammonia formation kinetic rate constant for immobilised enzyme, cPi the bulk Bradford protein concentration for immobilised enzymes, cBd the bulk concentration of dry beads and YP/B [I U /g d s] Figure 1: Effect of urea concentration (SL) on the specific ammonia formation rate of (A) free enzyme (πAE: ) at a free enzyme concentration (cPF) of 4.2 mg BSAE/L and (B) immobilised derivatives (πAB) with different enzyme loadings and immobilised enzyme concentrations: , YP/B = 48 mg BSAE/g ds and cBd = 0.11 mg BSAE/L; , YP/B	cache/cet-6583.pdf	txt/cet-6583.txt