id	sid	tid	token	lemma	pos
cet-12518	1	1	doi	doi	NOUN
cet-12518	1	2	:	:	PUNCT
cet-12518	1	3	10.3303	10.3303	NUM
cet-12518	1	4	/	/	SYM
cet-12518	1	5	cet2293021	cet2293021	NOUN
cet-12518	1	6	paper	paper	NOUN
cet-12518	1	7	received	receive	VERB
cet-12518	1	8	:	:	PUNCT
cet-12518	1	9	22	22	NUM
cet-12518	1	10	november	november	PROPN
cet-12518	1	11	2021	2021	NUM
cet-12518	1	12	;	;	PUNCT
cet-12518	1	13	revised	revise	VERB
cet-12518	1	14	:	:	PUNCT
cet-12518	1	15	3	3	NUM
cet-12518	1	16	march	march	NOUN
cet-12518	1	17	2022	2022	NUM
cet-12518	1	18	;	;	PUNCT
cet-12518	1	19	accepted	accept	VERB
cet-12518	1	20	:	:	PUNCT
cet-12518	1	21	9	9	NUM
cet-12518	1	22	may	may	AUX
cet-12518	1	23	2022	2022	NUM
cet-12518	1	24	please	please	INTJ
cet-12518	1	25	cite	cite	VERB
cet-12518	1	26	this	this	DET
cet-12518	1	27	article	article	NOUN
cet-12518	1	28	as	as	ADP
cet-12518	1	29	:	:	PUNCT
cet-12518	1	30	morales	morales	PROPN
cet-12518	1	31	-	-	PUNCT
cet-12518	1	32	fonseca	fonseca	PROPN
cet-12518	1	33	d.	d.	PROPN
cet-12518	1	34	,	,	PUNCT
cet-12518	1	35	coronado	coronado	PROPN
cet-12518	1	36	-	-	PUNCT
cet-12518	1	37	jimenez	jimenez	PROPN
cet-12518	1	38	l.	l.	PROPN
cet-12518	1	39	,	,	PUNCT
cet-12518	1	40	gonzalez	gonzalez	PROPN
cet-12518	1	41	-	-	PUNCT
cet-12518	1	42	moya	moya	PROPN
cet-12518	1	43	v.	v.	PROPN
cet-12518	1	44	,	,	PUNCT
cet-12518	1	45	zambrano	zambrano	PROPN
cet-12518	1	46	m.m	m.m	PROPN
cet-12518	1	47	.	.	PROPN
cet-12518	1	48	,	,	PUNCT
cet-12518	1	49	sandoval	sandoval	PROPN
cet-12518	1	50	-	-	PUNCT
cet-12518	1	51	herrera	herrera	PROPN
cet-12518	1	52	j.	j.	PROPN
cet-12518	1	53	,	,	PUNCT
cet-12518	1	54	arturocalvache	arturocalvache	PROPN
cet-12518	1	55	j.e	j.e	PROPN
cet-12518	1	56	.	.	PROPN
cet-12518	1	57	,	,	PUNCT
cet-12518	1	58	2022	2022	NUM
cet-12518	1	59	,	,	PUNCT
cet-12518	1	60	effect	effect	NOUN
cet-12518	1	61	of	of	ADP
cet-12518	1	62	agitation	agitation	NOUN
cet-12518	1	63	on	on	ADP
cet-12518	1	64	taq	taq	PROPN
cet-12518	1	65	dna	dna	PROPN
cet-12518	1	66	polymerase	polymerase	NOUN
cet-12518	1	67	production	production	NOUN
cet-12518	1	68	by	by	ADP
cet-12518	1	69	escherichia	escherichia	NOUN
cet-12518	1	70	coli	coli	NOUN
cet-12518	1	71	in	in	ADP
cet-12518	1	72	bioreactor	bioreactor	NOUN
cet-12518	1	73	,	,	PUNCT
cet-12518	1	74	chemical	chemical	NOUN
cet-12518	1	75	engineering	engineering	NOUN
cet-12518	1	76	transactions	transaction	NOUN
cet-12518	1	77	,	,	PUNCT
cet-12518	1	78	93	93	NUM
cet-12518	1	79	,	,	PUNCT
cet-12518	1	80	121	121	NUM
cet-12518	1	81	-	-	SYM
cet-12518	1	82	126	126	NUM
cet-12518	1	83	doi:10.3303	doi:10.3303	NOUN
cet-12518	1	84	/	/	SYM
cet-12518	1	85	cet2293021	cet2293021	NOUN
cet-12518	1	86	chemical	chemical	PROPN
cet-12518	1	87	engineering	engineering	NOUN
cet-12518	1	88	transactions	transaction	NOUN
cet-12518	1	89	vol	vol	NOUN
cet-12518	1	90	.	.	PUNCT
cet-12518	1	91	93	93	NUM
cet-12518	1	92	,	,	PUNCT
cet-12518	1	93	2022	2022	NUM
cet-12518	1	94	a	a	DET
cet-12518	1	95	publication	publication	NOUN
cet-12518	1	96	of	of	ADP
cet-12518	1	97	the	the	DET
cet-12518	1	98	italian	italian	ADJ
cet-12518	1	99	association	association	NOUN
cet-12518	1	100	of	of	ADP
cet-12518	1	101	chemical	chemical	PROPN
cet-12518	1	102	engineering	engineering	NOUN
cet-12518	1	103	online	online	ADV
cet-12518	1	104	at	at	ADP
cet-12518	1	105	www.cetjournal.it	www.cetjournal.it	PROPN
cet-12518	1	106	effect	effect	NOUN
cet-12518	1	107	of	of	ADP
cet-12518	1	108	agitation	agitation	NOUN
cet-12518	1	109	on	on	ADP
cet-12518	1	110	taq	taq	PROPN
cet-12518	1	111	dna	dna	PROPN
cet-12518	1	112	polymerase	polymerase	NOUN
cet-12518	1	113	production	production	NOUN
cet-12518	1	114	by	by	ADP
cet-12518	1	115	escherichia	escherichia	NOUN
cet-12518	1	116	coli	coli	NOUN
cet-12518	1	117	in	in	ADP
cet-12518	1	118	bioreactor	bioreactor	NOUN
cet-12518	1	119	diana	diana	PROPN
cet-12518	1	120	morales	morales	PROPN
cet-12518	1	121	-	-	PUNCT
cet-12518	1	122	fonseca*a	fonseca*a	PROPN
cet-12518	1	123	,	,	PUNCT
cet-12518	1	124	laura	laura	PROPN
cet-12518	1	125	coronado	coronado	PROPN
cet-12518	1	126	-	-	PUNCT
cet-12518	1	127	jimeneza	jimeneza	PROPN
cet-12518	1	128	,	,	PUNCT
cet-12518	1	129	valentina	valentina	PROPN
cet-12518	1	130	gonzalez	gonzalez	PROPN
cet-12518	1	131	-	-	PUNCT
cet-12518	1	132	moyaa	moyaa	PROPN
cet-12518	1	133	,	,	PUNCT
cet-12518	1	134	maría	maría	PROPN
cet-12518	1	135	mercedes	mercedes	PROPN
cet-12518	1	136	-	-	PUNCT
cet-12518	1	137	zambranob	zambranob	PROPN
cet-12518	1	138	,	,	PUNCT
cet-12518	1	139	juan	juan	PROPN
cet-12518	1	140	sandoval	sandoval	PROPN
cet-12518	1	141	-	-	PUNCT
cet-12518	1	142	herreraa	herreraa	NOUN
cet-12518	1	143	,	,	PUNCT
cet-12518	1	144	jaime	jaime	PROPN
cet-12518	1	145	e.	e.	PROPN
cet-12518	1	146	arturo	arturo	PROPN
cet-12518	1	147	-	-	PUNCT
cet-12518	1	148	calvachea	calvachea	PROPN
cet-12518	1	149	.	.	PUNCT
cet-12518	2	1	a	a	DET
cet-12518	2	2	department	department	NOUN
cet-12518	2	3	of	of	ADP
cet-12518	2	4	chemical	chemical	PROPN
cet-12518	2	5	and	and	CCONJ
cet-12518	2	6	environmental	environmental	ADJ
cet-12518	2	7	engineering	engineering	NOUN
cet-12518	2	8	,	,	PUNCT
cet-12518	2	9	fundación	fundación	PROPN
cet-12518	2	10	universidad	universidad	PROPN
cet-12518	2	11	de	de	PROPN
cet-12518	2	12	américa	américa	PROPN
cet-12518	2	13	,	,	PUNCT
cet-12518	2	14	bogotá	bogotá	NOUN
cet-12518	2	15	,	,	PUNCT
cet-12518	2	16	colombia	colombia	PROPN
cet-12518	2	17	.	.	PUNCT
cet-12518	3	1	bcorporación	bcorporación	PROPN
cet-12518	3	2	corpogen	corpogen	PROPN
cet-12518	3	3	,	,	PUNCT
cet-12518	3	4	bogotá	bogotá	NOUN
cet-12518	3	5	,	,	PUNCT
cet-12518	3	6	colombia	colombia	PROPN
cet-12518	3	7	.	.	PUNCT
cet-12518	4	1	diana.morales@profesores.uamerica.edu.co	diana.morales@profesores.uamerica.edu.co	PUNCT
cet-12518	4	2	the	the	DET
cet-12518	4	3	taq	taq	PROPN
cet-12518	4	4	dna	dna	PROPN
cet-12518	4	5	polymerase	polymerase	NOUN
cet-12518	4	6	is	be	AUX
cet-12518	4	7	an	an	DET
cet-12518	4	8	important	important	ADJ
cet-12518	4	9	protein	protein	NOUN
cet-12518	4	10	used	use	VERB
cet-12518	4	11	during	during	ADP
cet-12518	4	12	pcr	pcr	PROPN
cet-12518	4	13	(	(	PUNCT
cet-12518	4	14	polymerase	polymerase	NOUN
cet-12518	4	15	chain	chain	NOUN
cet-12518	4	16	reaction	reaction	NOUN
cet-12518	4	17	)	)	PUNCT
cet-12518	4	18	,	,	PUNCT
cet-12518	4	19	a	a	DET
cet-12518	4	20	technique	technique	NOUN
cet-12518	4	21	widely	widely	ADV
cet-12518	4	22	used	use	VERB
cet-12518	4	23	today	today	NOUN
cet-12518	4	24	.	.	PUNCT
cet-12518	5	1	the	the	DET
cet-12518	5	2	production	production	NOUN
cet-12518	5	3	of	of	ADP
cet-12518	5	4	this	this	DET
cet-12518	5	5	enzyme	enzyme	NOUN
cet-12518	5	6	in	in	ADP
cet-12518	5	7	bioreactors	bioreactor	NOUN
cet-12518	5	8	make	make	VERB
cet-12518	5	9	possible	possible	ADJ
cet-12518	5	10	to	to	PART
cet-12518	5	11	improve	improve	VERB
cet-12518	5	12	efficiency	efficiency	NOUN
cet-12518	5	13	,	,	PUNCT
cet-12518	5	14	to	to	PART
cet-12518	5	15	reduce	reduce	VERB
cet-12518	5	16	production	production	NOUN
cet-12518	5	17	time	time	NOUN
cet-12518	5	18	,	,	PUNCT
cet-12518	5	19	to	to	PART
cet-12518	5	20	increase	increase	VERB
cet-12518	5	21	production	production	NOUN
cet-12518	5	22	and	and	CCONJ
cet-12518	5	23	expression	expression	NOUN
cet-12518	5	24	,	,	PUNCT
cet-12518	5	25	or	or	CCONJ
cet-12518	5	26	to	to	PART
cet-12518	5	27	reduce	reduce	VERB
cet-12518	5	28	production	production	NOUN
cet-12518	5	29	costs	cost	NOUN
cet-12518	5	30	.	.	PUNCT
cet-12518	6	1	this	this	PRON
cet-12518	6	2	is	be	AUX
cet-12518	6	3	achieved	achieve	VERB
cet-12518	6	4	thanks	thank	NOUN
cet-12518	6	5	to	to	ADP
cet-12518	6	6	the	the	DET
cet-12518	6	7	measurement	measurement	NOUN
cet-12518	6	8	,	,	PUNCT
cet-12518	6	9	monitoring	monitoring	NOUN
cet-12518	6	10	and	and	CCONJ
cet-12518	6	11	control	control	NOUN
cet-12518	6	12	of	of	ADP
cet-12518	6	13	the	the	DET
cet-12518	6	14	different	different	ADJ
cet-12518	6	15	parameters	parameter	NOUN
cet-12518	6	16	that	that	PRON
cet-12518	6	17	influence	influence	VERB
cet-12518	6	18	this	this	DET
cet-12518	6	19	biotechnological	biotechnological	ADJ
cet-12518	6	20	process	process	NOUN
cet-12518	6	21	:	:	PUNCT
cet-12518	6	22	dissolved	dissolve	VERB
cet-12518	6	23	oxygen	oxygen	NOUN
cet-12518	6	24	,	,	PUNCT
cet-12518	6	25	ph	ph	VERB
cet-12518	6	26	,	,	PUNCT
cet-12518	6	27	temperature	temperature	NOUN
cet-12518	6	28	,	,	PUNCT
cet-12518	6	29	incubation	incubation	NOUN
cet-12518	6	30	time	time	NOUN
cet-12518	6	31	,	,	PUNCT
cet-12518	6	32	agitation	agitation	NOUN
cet-12518	6	33	during	during	ADP
cet-12518	6	34	the	the	DET
cet-12518	6	35	incubation	incubation	NOUN
cet-12518	6	36	and	and	CCONJ
cet-12518	6	37	induction	induction	NOUN
cet-12518	6	38	phase.in	phase.in	PRON
cet-12518	6	39	the	the	DET
cet-12518	6	40	present	present	ADJ
cet-12518	6	41	work	work	NOUN
cet-12518	6	42	,	,	PUNCT
cet-12518	6	43	the	the	DET
cet-12518	6	44	production	production	NOUN
cet-12518	6	45	of	of	ADP
cet-12518	6	46	taq	taq	NOUN
cet-12518	6	47	polymerase	polymerase	NOUN
cet-12518	6	48	expressed	express	VERB
cet-12518	6	49	in	in	ADP
cet-12518	6	50	escherichia	escherichia	NOUN
cet-12518	6	51	coli	coli	NOUN
cet-12518	6	52	,	,	PUNCT
cet-12518	6	53	was	be	AUX
cet-12518	6	54	made	make	VERB
cet-12518	6	55	on	on	ADP
cet-12518	6	56	a	a	DET
cet-12518	6	57	bioreactor	bioreactor	NOUN
cet-12518	6	58	scale	scale	NOUN
cet-12518	6	59	,	,	PUNCT
cet-12518	6	60	bioflo	bioflo	NOUN
cet-12518	6	61	/	/	SYM
cet-12518	6	62	celligen	celligen	PROPN
cet-12518	6	63	115	115	NUM
cet-12518	6	64	®	®	PROPN
cet-12518	6	65	,	,	PUNCT
cet-12518	6	66	with	with	ADP
cet-12518	6	67	a	a	DET
cet-12518	6	68	volume	volume	NOUN
cet-12518	6	69	of	of	ADP
cet-12518	6	70	1	1	NUM
cet-12518	6	71	l	l	NOUN
cet-12518	6	72	,	,	PUNCT
cet-12518	6	73	equipped	equip	VERB
cet-12518	6	74	with	with	ADP
cet-12518	6	75	temperature	temperature	NOUN
cet-12518	6	76	(	(	PUNCT
cet-12518	6	77	37	37	NUM
cet-12518	6	78	°	°	NOUN
cet-12518	6	79	c	c	NOUN
cet-12518	6	80	)	)	PUNCT
cet-12518	6	81	and	and	CCONJ
cet-12518	6	82	ph	ph	NOUN
cet-12518	6	83	(	(	PUNCT
cet-12518	6	84	6.5	6.5	NUM
cet-12518	6	85	)	)	PUNCT
cet-12518	6	86	control	control	NOUN
cet-12518	6	87	,	,	PUNCT
cet-12518	6	88	and	and	CCONJ
cet-12518	6	89	with	with	ADP
cet-12518	6	90	a	a	DET
cet-12518	6	91	constant	constant	ADJ
cet-12518	6	92	level	level	NOUN
cet-12518	6	93	of	of	ADP
cet-12518	6	94	dissolved	dissolve	VERB
cet-12518	6	95	oxygen	oxygen	NOUN
cet-12518	6	96	provided	provide	VERB
cet-12518	6	97	by	by	ADP
cet-12518	6	98	a	a	DET
cet-12518	6	99	1	1	NUM
cet-12518	6	100	vvm	vvm	NOUN
cet-12518	6	101	flow	flow	NOUN
cet-12518	6	102	of	of	ADP
cet-12518	6	103	air	air	NOUN
cet-12518	6	104	.	.	PUNCT
cet-12518	7	1	after	after	ADP
cet-12518	7	2	the	the	DET
cet-12518	7	3	induction	induction	NOUN
cet-12518	7	4	phase	phase	NOUN
cet-12518	7	5	,	,	PUNCT
cet-12518	7	6	we	we	PRON
cet-12518	7	7	extracted	extract	VERB
cet-12518	7	8	the	the	DET
cet-12518	7	9	polymerase	polymerase	NOUN
cet-12518	7	10	by	by	ADP
cet-12518	7	11	cell	cell	NOUN
cet-12518	7	12	disruption	disruption	NOUN
cet-12518	7	13	using	use	VERB
cet-12518	7	14	centrifugation	centrifugation	NOUN
cet-12518	7	15	and	and	CCONJ
cet-12518	7	16	some	some	DET
cet-12518	7	17	substances	substance	NOUN
cet-12518	7	18	.	.	PUNCT
cet-12518	8	1	for	for	SCONJ
cet-12518	8	2	the	the	DET
cet-12518	8	3	protein	protein	NOUN
cet-12518	8	4	measurement	measurement	NOUN
cet-12518	8	5	was	be	AUX
cet-12518	8	6	implemented	implement	VERB
cet-12518	8	7	d	d	PRON
cet-12518	8	8	technique	technique	NOUN
cet-12518	8	9	and	and	CCONJ
cet-12518	8	10	sds	sds	NOUN
cet-12518	8	11	-	-	PUNCT
cet-12518	8	12	page	page	NOUN
cet-12518	8	13	electrophoresis	electrophoresis	NOUN
cet-12518	8	14	.	.	PUNCT
cet-12518	9	1	according	accord	VERB
cet-12518	9	2	to	to	ADP
cet-12518	9	3	gel	gel	NOUN
cet-12518	9	4	electrophoresis	electrophoresis	NOUN
cet-12518	9	5	results	result	NOUN
cet-12518	9	6	,	,	PUNCT
cet-12518	9	7	molecular	molecular	ADJ
cet-12518	9	8	weight	weight	NOUN
cet-12518	9	9	of	of	ADP
cet-12518	9	10	the	the	DET
cet-12518	9	11	protein	protein	NOUN
cet-12518	9	12	obtained	obtain	VERB
cet-12518	9	13	in	in	ADP
cet-12518	9	14	bioreactor	bioreactor	NOUN
cet-12518	9	15	was	be	AUX
cet-12518	9	16	80	80	NUM
cet-12518	9	17	kd	kd	NOUN
cet-12518	9	18	,	,	PUNCT
cet-12518	9	19	similar	similar	ADJ
cet-12518	9	20	to	to	ADP
cet-12518	9	21	the	the	DET
cet-12518	9	22	value	value	NOUN
cet-12518	9	23	of	of	ADP
cet-12518	9	24	commercial	commercial	ADJ
cet-12518	9	25	production	production	NOUN
cet-12518	9	26	in	in	ADP
cet-12518	9	27	flasks	flask	NOUN
cet-12518	9	28	.	.	PUNCT
cet-12518	10	1	the	the	DET
cet-12518	10	2	effect	effect	NOUN
cet-12518	10	3	of	of	ADP
cet-12518	10	4	agitation	agitation	NOUN
cet-12518	10	5	on	on	ADP
cet-12518	10	6	protein	protein	NOUN
cet-12518	10	7	concentration	concentration	NOUN
cet-12518	10	8	after	after	SCONJ
cet-12518	10	9	induction	induction	NOUN
cet-12518	10	10	was	be	AUX
cet-12518	10	11	studied	study	VERB
cet-12518	10	12	at	at	ADP
cet-12518	10	13	two	two	NUM
cet-12518	10	14	levels	level	NOUN
cet-12518	10	15	:	:	PUNCT
cet-12518	10	16	220	220	NUM
cet-12518	10	17	and	and	CCONJ
cet-12518	10	18	350	350	NUM
cet-12518	10	19	rpm	rpm	NOUN
cet-12518	10	20	,	,	PUNCT
cet-12518	10	21	the	the	DET
cet-12518	10	22	study	study	NOUN
cet-12518	10	23	found	find	VERB
cet-12518	10	24	a	a	DET
cet-12518	10	25	production	production	NOUN
cet-12518	10	26	of	of	ADP
cet-12518	10	27	1465	1465	NUM
cet-12518	10	28	𝜇𝜇𝜇𝜇	𝜇𝜇𝜇𝜇	NOUN
cet-12518	10	29	𝐿𝐿−1	𝐿𝐿−1	NOUN
cet-12518	10	30	and	and	CCONJ
cet-12518	10	31	2975	2975	NUM
cet-12518	10	32	𝜇𝜇𝜇𝜇	𝜇𝜇𝜇𝜇	PROPN
cet-12518	10	33	𝐿𝐿−1	𝐿𝐿−1	NOUN
cet-12518	10	34	of	of	ADP
cet-12518	10	35	protein	protein	NOUN
cet-12518	10	36	respectively	respectively	ADV
cet-12518	10	37	.	.	PUNCT
cet-12518	11	1	the	the	DET
cet-12518	11	2	results	result	NOUN
cet-12518	11	3	confirm	confirm	VERB
cet-12518	11	4	a	a	DET
cet-12518	11	5	higher	high	ADJ
cet-12518	11	6	production	production	NOUN
cet-12518	11	7	of	of	ADP
cet-12518	11	8	protein	protein	NOUN
cet-12518	11	9	in	in	ADP
cet-12518	11	10	this	this	DET
cet-12518	11	11	bioreactor	bioreactor	NOUN
cet-12518	11	12	,	,	PUNCT
cet-12518	11	13	keeping	keep	VERB
cet-12518	11	14	the	the	DET
cet-12518	11	15	type	type	NOUN
cet-12518	11	16	of	of	ADP
cet-12518	11	17	polymerase	polymerase	NOUN
cet-12518	11	18	.	.	PUNCT
cet-12518	12	1	this	this	PRON
cet-12518	12	2	could	could	AUX
cet-12518	12	3	be	be	AUX
cet-12518	12	4	the	the	DET
cet-12518	12	5	first	first	ADJ
cet-12518	12	6	step	step	NOUN
cet-12518	12	7	to	to	PART
cet-12518	12	8	scale	scale	VERB
cet-12518	12	9	the	the	DET
cet-12518	12	10	production	production	NOUN
cet-12518	12	11	with	with	ADP
cet-12518	12	12	controlled	control	VERB
cet-12518	12	13	conditions	condition	NOUN
cet-12518	12	14	at	at	ADP
cet-12518	12	15	higher	high	ADJ
cet-12518	12	16	rates	rate	NOUN
cet-12518	12	17	.	.	PUNCT
cet-12518	13	1	1	1	X
cet-12518	13	2	.	.	X
cet-12518	13	3	introduction	introduction	NOUN
cet-12518	13	4	the	the	DET
cet-12518	13	5	production	production	NOUN
cet-12518	13	6	of	of	ADP
cet-12518	13	7	heterologous	heterologous	ADJ
cet-12518	13	8	proteins	protein	NOUN
cet-12518	13	9	on	on	ADP
cet-12518	13	10	a	a	DET
cet-12518	13	11	bank	bank	NOUN
cet-12518	13	12	and	and	CCONJ
cet-12518	13	13	industrial	industrial	ADJ
cet-12518	13	14	scale	scale	NOUN
cet-12518	13	15	represents	represent	VERB
cet-12518	13	16	the	the	DET
cet-12518	13	17	opportunity	opportunity	NOUN
cet-12518	13	18	to	to	PART
cet-12518	13	19	design	design	VERB
cet-12518	13	20	bioprocess	bioprocess	NOUN
cet-12518	13	21	with	with	ADP
cet-12518	13	22	higher	high	ADJ
cet-12518	13	23	yields	yield	NOUN
cet-12518	13	24	and	and	CCONJ
cet-12518	13	25	profitability	profitability	NOUN
cet-12518	13	26	that	that	PRON
cet-12518	13	27	allow	allow	VERB
cet-12518	13	28	more	more	ADV
cet-12518	13	29	accessible	accessible	ADJ
cet-12518	13	30	commercial	commercial	ADJ
cet-12518	13	31	values	value	NOUN
cet-12518	13	32	,	,	PUNCT
cet-12518	13	33	especially	especially	ADV
cet-12518	13	34	in	in	ADP
cet-12518	13	35	the	the	DET
cet-12518	13	36	field	field	NOUN
cet-12518	13	37	of	of	ADP
cet-12518	13	38	industrial	industrial	ADJ
cet-12518	13	39	biotechnology	biotechnology	NOUN
cet-12518	13	40	in	in	ADP
cet-12518	13	41	colombia	colombia	PROPN
cet-12518	13	42	.	.	PUNCT
cet-12518	14	1	the	the	DET
cet-12518	14	2	taq	taq	PROPN
cet-12518	14	3	dna	dna	PROPN
cet-12518	14	4	polymerase	polymerase	NOUN
cet-12518	14	5	is	be	AUX
cet-12518	14	6	an	an	DET
cet-12518	14	7	essential	essential	ADJ
cet-12518	14	8	enzyme	enzyme	NOUN
cet-12518	14	9	used	use	VERB
cet-12518	14	10	to	to	PART
cet-12518	14	11	amplify	amplify	VERB
cet-12518	14	12	dna	dna	PROPN
cet-12518	14	13	sequences	sequence	NOUN
cet-12518	14	14	by	by	ADP
cet-12518	14	15	pcr	pcr	ADJ
cet-12518	14	16	technique	technique	NOUN
cet-12518	14	17	,	,	PUNCT
cet-12518	14	18	the	the	DET
cet-12518	14	19	enzyme	enzyme	NOUN
cet-12518	14	20	was	be	AUX
cet-12518	14	21	extracted	extract	VERB
cet-12518	14	22	from	from	ADP
cet-12518	14	23	thermophilic	thermophilic	ADJ
cet-12518	14	24	bacteria	bacteria	NOUN
cet-12518	14	25	and	and	CCONJ
cet-12518	14	26	for	for	ADP
cet-12518	14	27	its	its	PRON
cet-12518	14	28	production	production	NOUN
cet-12518	14	29	it	it	PRON
cet-12518	14	30	is	be	AUX
cet-12518	14	31	expressed	express	VERB
cet-12518	14	32	in	in	ADP
cet-12518	14	33	a	a	DET
cet-12518	14	34	fast	fast	ADV
cet-12518	14	35	-	-	PUNCT
cet-12518	14	36	growing	grow	VERB
cet-12518	14	37	bacteria	bacteria	NOUN
cet-12518	14	38	escherichia	escherichia	NOUN
cet-12518	14	39	coli	coli	NOUN
cet-12518	14	40	as	as	ADP
cet-12518	14	41	heterologous	heterologous	ADJ
cet-12518	14	42	protein	protein	NOUN
cet-12518	14	43	(	(	PUNCT
cet-12518	14	44	sung	sung	NOUN
cet-12518	14	45	-	-	PUNCT
cet-12518	14	46	gun	gun	NOUN
cet-12518	14	47	and	and	CCONJ
cet-12518	14	48	jong	jong	PROPN
cet-12518	14	49	-	-	PUNCT
cet-12518	14	50	tae	tae	PROPN
cet-12518	14	51	,	,	PUNCT
cet-12518	14	52	2014	2014	NUM
cet-12518	14	53	)	)	PUNCT
cet-12518	14	54	.	.	PUNCT
cet-12518	15	1	several	several	ADJ
cet-12518	15	2	factors	factor	NOUN
cet-12518	15	3	affect	affect	VERB
cet-12518	15	4	the	the	DET
cet-12518	15	5	expression	expression	NOUN
cet-12518	15	6	of	of	ADP
cet-12518	15	7	a	a	DET
cet-12518	15	8	metabolite	metabolite	NOUN
cet-12518	15	9	of	of	ADP
cet-12518	15	10	interest	interest	NOUN
cet-12518	15	11	on	on	ADP
cet-12518	15	12	the	the	DET
cet-12518	15	13	part	part	NOUN
cet-12518	15	14	of	of	ADP
cet-12518	15	15	a	a	DET
cet-12518	15	16	microorganism	microorganism	NOUN
cet-12518	15	17	,	,	PUNCT
cet-12518	15	18	aspects	aspect	NOUN
cet-12518	15	19	such	such	ADJ
cet-12518	15	20	as	as	ADP
cet-12518	15	21	metabolism	metabolism	NOUN
cet-12518	15	22	,	,	PUNCT
cet-12518	15	23	genetic	genetic	ADJ
cet-12518	15	24	regulation	regulation	NOUN
cet-12518	15	25	,	,	PUNCT
cet-12518	15	26	and	and	CCONJ
cet-12518	15	27	the	the	DET
cet-12518	15	28	conditions	condition	NOUN
cet-12518	15	29	of	of	ADP
cet-12518	15	30	bioreactor	bioreactor	NOUN
cet-12518	15	31	operation	operation	NOUN
cet-12518	15	32	,	,	PUNCT
cet-12518	15	33	it	it	PRON
cet-12518	15	34	is	be	AUX
cet-12518	15	35	important	important	ADJ
cet-12518	15	36	to	to	PART
cet-12518	15	37	consider	consider	VERB
cet-12518	15	38	criteria	criterion	NOUN
cet-12518	15	39	such	such	ADJ
cet-12518	15	40	as	as	ADP
cet-12518	15	41	volume	volume	NOUN
cet-12518	15	42	,	,	PUNCT
cet-12518	15	43	temperature	temperature	NOUN
cet-12518	15	44	,	,	PUNCT
cet-12518	15	45	aeration	aeration	NOUN
cet-12518	15	46	,	,	PUNCT
cet-12518	15	47	and	and	CCONJ
cet-12518	15	48	agitation	agitation	NOUN
cet-12518	15	49	of	of	ADP
cet-12518	15	50	the	the	DET
cet-12518	15	51	system	system	NOUN
cet-12518	15	52	,	,	PUNCT
cet-12518	15	53	among	among	ADP
cet-12518	15	54	others	other	NOUN
cet-12518	15	55	.	.	PUNCT
cet-12518	16	1	in	in	ADP
cet-12518	16	2	the	the	DET
cet-12518	16	3	case	case	NOUN
cet-12518	16	4	of	of	ADP
cet-12518	16	5	products	product	NOUN
cet-12518	16	6	obtained	obtain	VERB
cet-12518	16	7	by	by	ADP
cet-12518	16	8	recombinant	recombinant	ADJ
cet-12518	16	9	dna	dna	PROPN
cet-12518	16	10	technology	technology	NOUN
cet-12518	16	11	,	,	PUNCT
cet-12518	16	12	it	it	PRON
cet-12518	16	13	is	be	AUX
cet-12518	16	14	necessary	necessary	ADJ
cet-12518	16	15	to	to	PART
cet-12518	16	16	specify	specify	VERB
cet-12518	16	17	how	how	SCONJ
cet-12518	16	18	these	these	DET
cet-12518	16	19	conditions	condition	NOUN
cet-12518	16	20	affect	affect	VERB
cet-12518	16	21	the	the	DET
cet-12518	16	22	stability	stability	NOUN
cet-12518	16	23	of	of	ADP
cet-12518	16	24	the	the	DET
cet-12518	16	25	plasmid	plasmid	NOUN
cet-12518	16	26	reflected	reflect	VERB
cet-12518	16	27	in	in	ADP
cet-12518	16	28	the	the	DET
cet-12518	16	29	expression	expression	NOUN
cet-12518	16	30	of	of	ADP
cet-12518	16	31	the	the	DET
cet-12518	16	32	protein	protein	NOUN
cet-12518	16	33	(	(	PUNCT
cet-12518	16	34	che	che	X
cet-12518	16	35	et	et	PROPN
cet-12518	16	36	al	al	PROPN
cet-12518	16	37	.	.	PROPN
cet-12518	16	38	,	,	PUNCT
cet-12518	16	39	2016	2016	NUM
cet-12518	16	40	)	)	PUNCT
cet-12518	16	41	.	.	PUNCT
cet-12518	17	1	the	the	DET
cet-12518	17	2	production	production	NOUN
cet-12518	17	3	of	of	ADP
cet-12518	17	4	taq	taq	NOUN
cet-12518	17	5	polymerase	polymerase	NOUN
cet-12518	17	6	is	be	AUX
cet-12518	17	7	documented	document	VERB
cet-12518	17	8	at	at	ADP
cet-12518	17	9	laboratory	laboratory	NOUN
cet-12518	17	10	scale	scale	NOUN
cet-12518	17	11	in	in	ADP
cet-12518	17	12	flasks	flask	NOUN
cet-12518	17	13	;	;	PUNCT
cet-12518	17	14	however	however	ADV
cet-12518	17	15	,	,	PUNCT
cet-12518	17	16	this	this	PRON
cet-12518	17	17	limits	limit	VERB
cet-12518	17	18	the	the	DET
cet-12518	17	19	options	option	NOUN
cet-12518	17	20	to	to	PART
cet-12518	17	21	constantly	constantly	ADV
cet-12518	17	22	monitor	monitor	VERB
cet-12518	17	23	and	and	CCONJ
cet-12518	17	24	control	control	VERB
cet-12518	17	25	the	the	DET
cet-12518	17	26	evolution	evolution	NOUN
cet-12518	17	27	of	of	ADP
cet-12518	17	28	the	the	DET
cet-12518	17	29	fermentation	fermentation	NOUN
cet-12518	17	30	process	process	NOUN
cet-12518	17	31	.	.	PUNCT
cet-12518	18	1	bioreactors	bioreactor	NOUN
cet-12518	18	2	allow	allow	VERB
cet-12518	18	3	the	the	DET
cet-12518	18	4	development	development	NOUN
cet-12518	18	5	of	of	ADP
cet-12518	18	6	different	different	ADJ
cet-12518	18	7	biotechnological	biotechnological	ADJ
cet-12518	18	8	processes	process	NOUN
cet-12518	18	9	because	because	SCONJ
cet-12518	18	10	they	they	PRON
cet-12518	18	11	offer	offer	VERB
cet-12518	18	12	operational	operational	ADJ
cet-12518	18	13	control	control	NOUN
cet-12518	18	14	at	at	ADP
cet-12518	18	15	different	different	ADJ
cet-12518	18	16	scales	scale	NOUN
cet-12518	18	17	(	(	PUNCT
cet-12518	18	18	liu	liu	PROPN
cet-12518	18	19	.	.	PROPN
cet-12518	18	20	,2020	,2020	PROPN
cet-12518	18	21	)	)	PUNCT
cet-12518	18	22	.	.	PUNCT
cet-12518	19	1	the	the	DET
cet-12518	19	2	bioreactor	bioreactor	NOUN
cet-12518	19	3	used	use	VERB
cet-12518	19	4	in	in	ADP
cet-12518	19	5	this	this	DET
cet-12518	19	6	work	work	NOUN
cet-12518	19	7	is	be	AUX
cet-12518	19	8	the	the	DET
cet-12518	19	9	bioflo	bioflo	NOUN
cet-12518	19	10	/	/	SYM
cet-12518	19	11	celligen	celligen	PROPN
cet-12518	19	12	115	115	NUM
cet-12518	19	13	®	®	NOUN
cet-12518	19	14	,	,	PUNCT
cet-12518	19	15	a	a	DET
cet-12518	19	16	continuous	continuous	ADJ
cet-12518	19	17	agitation	agitation	NOUN
cet-12518	19	18	bioreactor	bioreactor	NOUN
cet-12518	19	19	,	,	PUNCT
cet-12518	19	20	which	which	PRON
cet-12518	19	21	provides	provide	VERB
cet-12518	19	22	process	process	NOUN
cet-12518	19	23	control	control	NOUN
cet-12518	19	24	for	for	ADP
cet-12518	19	25	ph	ph	NOUN
cet-12518	19	26	,	,	PUNCT
cet-12518	19	27	agitation	agitation	NOUN
cet-12518	19	28	,	,	PUNCT
cet-12518	19	29	dissolved	dissolve	VERB
cet-12518	19	30	oxygen	oxygen	NOUN
cet-12518	19	31	and	and	CCONJ
cet-12518	19	32	temperature	temperature	NOUN
cet-12518	19	33	,	,	PUNCT
cet-12518	19	34	and	and	CCONJ
cet-12518	19	35	in	in	ADP
cet-12518	19	36	turn	turn	NOUN
cet-12518	19	37	has	have	VERB
cet-12518	19	38	the	the	DET
cet-12518	19	39	biocommand	biocommand	NOUN
cet-12518	19	40	software	software	NOUN
cet-12518	19	41	,	,	PUNCT
cet-12518	19	42	which	which	PRON
cet-12518	19	43	allows	allow	VERB
cet-12518	19	44	monitoring	monitor	VERB
cet-12518	19	45	environmental	environmental	ADJ
cet-12518	19	46	parameters	parameter	NOUN
cet-12518	19	47	(	(	PUNCT
cet-12518	19	48	eppendorf	eppendorf	PROPN
cet-12518	19	49	.	.	PROPN
cet-12518	19	50	,	,	PUNCT
cet-12518	19	51	2009	2009	NUM
cet-12518	19	52	)	)	PUNCT
cet-12518	19	53	.	.	PUNCT
cet-12518	20	1	guest	guest	NOUN
cet-12518	20	2	editors	editor	NOUN
cet-12518	20	3	:	:	PUNCT
cet-12518	20	4	marco	marco	PROPN
cet-12518	20	5	bravi	bravi	PROPN
cet-12518	20	6	,	,	PUNCT
cet-12518	20	7	alberto	alberto	PROPN
cet-12518	20	8	brucato	brucato	PROPN
cet-12518	20	9	,	,	PUNCT
cet-12518	20	10	antonio	antonio	PROPN
cet-12518	20	11	marzocchella	marzocchella	NOUN
cet-12518	20	12	copyright	copyright	NOUN
cet-12518	20	13	©	©	PROPN
cet-12518	20	14	2022	2022	NUM
cet-12518	20	15	,	,	PUNCT
cet-12518	20	16	aidic	aidic	ADJ
cet-12518	20	17	servizi	servizi	PROPN
cet-12518	20	18	s.r.l	s.r.l	PROPN
cet-12518	20	19	.	.	PUNCT
cet-12518	21	1	isbn	isbn	ADJ
cet-12518	21	2	978	978	NUM
cet-12518	21	3	-	-	SYM
cet-12518	21	4	88	88	NUM
cet-12518	21	5	-	-	PUNCT
cet-12518	21	6	95608	95608	NUM
cet-12518	21	7	-	-	PUNCT
cet-12518	21	8	91	91	NUM
cet-12518	21	9	-	-	PUNCT
cet-12518	21	10	4	4	NUM
cet-12518	21	11	;	;	PUNCT
cet-12518	21	12	issn	issn	PROPN
cet-12518	21	13	2283	2283	NUM
cet-12518	21	14	-	-	SYM
cet-12518	21	15	9216	9216	NUM
cet-12518	21	16	121	121	NUM
cet-12518	21	17	the	the	DET
cet-12518	21	18	main	main	ADJ
cet-12518	21	19	objective	objective	NOUN
cet-12518	21	20	of	of	ADP
cet-12518	21	21	this	this	DET
cet-12518	21	22	work	work	NOUN
cet-12518	21	23	was	be	AUX
cet-12518	21	24	to	to	PART
cet-12518	21	25	study	study	VERB
cet-12518	21	26	the	the	DET
cet-12518	21	27	effect	effect	NOUN
cet-12518	21	28	of	of	ADP
cet-12518	21	29	agitation	agitation	NOUN
cet-12518	21	30	on	on	ADP
cet-12518	21	31	the	the	DET
cet-12518	21	32	production	production	NOUN
cet-12518	21	33	of	of	ADP
cet-12518	21	34	the	the	DET
cet-12518	21	35	commercial	commercial	ADJ
cet-12518	21	36	enzyme	enzyme	NOUN
cet-12518	21	37	taq	taq	NOUN
cet-12518	21	38	polymerase	polymerase	NOUN
cet-12518	21	39	tucan	tucan	PROPN
cet-12518	21	40	taq	taq	PROPN
cet-12518	21	41	®	®	NOUN
cet-12518	21	42	from	from	ADP
cet-12518	21	43	a	a	DET
cet-12518	21	44	recombinant	recombinant	ADJ
cet-12518	21	45	strain	strain	NOUN
cet-12518	21	46	of	of	ADP
cet-12518	21	47	e.	e.	PROPN
cet-12518	21	48	coli	coli	NOUN
cet-12518	21	49	in	in	ADP
cet-12518	21	50	a	a	DET
cet-12518	21	51	bioreactor	bioreactor	NOUN
cet-12518	21	52	with	with	ADP
cet-12518	21	53	an	an	DET
cet-12518	21	54	effective	effective	ADJ
cet-12518	21	55	volume	volume	NOUN
cet-12518	21	56	of	of	ADP
cet-12518	21	57	400	400	NUM
cet-12518	21	58	ml	ml	NOUN
cet-12518	21	59	,	,	PUNCT
cet-12518	21	60	keeping	keep	VERB
cet-12518	21	61	an	an	DET
cet-12518	21	62	airflow	airflow	NOUN
cet-12518	21	63	of	of	ADP
cet-12518	21	64	1	1	NUM
cet-12518	21	65	vvm	vvm	NOUN
cet-12518	21	66	.	.	PUNCT
cet-12518	22	1	the	the	DET
cet-12518	22	2	results	result	NOUN
cet-12518	22	3	of	of	ADP
cet-12518	22	4	the	the	DET
cet-12518	22	5	enzymatic	enzymatic	ADJ
cet-12518	22	6	expression	expression	NOUN
cet-12518	22	7	were	be	AUX
cet-12518	22	8	compared	compare	VERB
cet-12518	22	9	with	with	ADP
cet-12518	22	10	the	the	DET
cet-12518	22	11	laboratoryscale	laboratoryscale	ADJ
cet-12518	22	12	production	production	NOUN
cet-12518	22	13	in	in	ADP
cet-12518	22	14	shake	shake	NOUN
cet-12518	22	15	flasks	flask	NOUN
cet-12518	22	16	to	to	PART
cet-12518	22	17	establish	establish	VERB
cet-12518	22	18	that	that	SCONJ
cet-12518	22	19	the	the	DET
cet-12518	22	20	production	production	NOUN
cet-12518	22	21	on	on	ADP
cet-12518	22	22	a	a	DET
cet-12518	22	23	larger	large	ADJ
cet-12518	22	24	scale	scale	NOUN
cet-12518	22	25	does	do	AUX
cet-12518	22	26	not	not	PART
cet-12518	22	27	alter	alter	VERB
cet-12518	22	28	the	the	DET
cet-12518	22	29	stability	stability	NOUN
cet-12518	22	30	of	of	ADP
cet-12518	22	31	the	the	DET
cet-12518	22	32	plasmid	plasmid	NOUN
cet-12518	22	33	and	and	CCONJ
cet-12518	22	34	the	the	DET
cet-12518	22	35	bioproduct	bioproduct	NOUN
cet-12518	22	36	.	.	PUNCT
cet-12518	23	1	2	2	X
cet-12518	23	2	.	.	X
cet-12518	23	3	materials	material	NOUN
cet-12518	23	4	and	and	CCONJ
cet-12518	23	5	method	method	VERB
cet-12518	23	6	2.1	2.1	NUM
cet-12518	23	7	microorganism	microorganism	NOUN
cet-12518	23	8	e.	e.	PROPN
cet-12518	23	9	coli	coli	NOUN
cet-12518	23	10	strain	strain	NOUN
cet-12518	23	11	dh5α	dh5α	PROPN
cet-12518	23	12	was	be	AUX
cet-12518	23	13	transformed	transform	VERB
cet-12518	23	14	with	with	ADP
cet-12518	23	15	a	a	DET
cet-12518	23	16	plasmid	plasmid	NOUN
cet-12518	23	17	which	which	PRON
cet-12518	23	18	contains	contain	VERB
cet-12518	23	19	a	a	DET
cet-12518	23	20	taq	taq	NOUN
cet-12518	23	21	polymerase	polymerase	NOUN
cet-12518	23	22	gene	gene	NOUN
cet-12518	23	23	.	.	PUNCT
cet-12518	24	1	transformation	transformation	NOUN
cet-12518	24	2	and	and	CCONJ
cet-12518	24	3	activation	activation	NOUN
cet-12518	24	4	were	be	AUX
cet-12518	24	5	performed	perform	VERB
cet-12518	24	6	in	in	ADP
cet-12518	24	7	broth	broth	NOUN
cet-12518	24	8	lb	lb	ADP
cet-12518	24	9	(	(	PUNCT
cet-12518	24	10	10	10	NUM
cet-12518	24	11	g	g	NOUN
cet-12518	24	12	/	/	SYM
cet-12518	24	13	l	l	NOUN
cet-12518	24	14	triptone	triptone	NOUN
cet-12518	24	15	,	,	PUNCT
cet-12518	24	16	5	5	NUM
cet-12518	24	17	g	g	NOUN
cet-12518	24	18	/	/	SYM
cet-12518	24	19	l	l	NOUN
cet-12518	24	20	yeast	yeast	NOUN
cet-12518	24	21	extract	extract	NOUN
cet-12518	24	22	,	,	PUNCT
cet-12518	24	23	10	10	NUM
cet-12518	24	24	g	g	NOUN
cet-12518	24	25	/	/	SYM
cet-12518	24	26	l	l	NOUN
cet-12518	24	27	sodium	sodium	NOUN
cet-12518	24	28	chloride	chloride	NOUN
cet-12518	24	29	)	)	PUNCT
cet-12518	24	30	(	(	PUNCT
cet-12518	24	31	merck	merck	NOUN
cet-12518	24	32	,	,	PUNCT
cet-12518	24	33	2020	2020	NUM
cet-12518	24	34	)	)	PUNCT
cet-12518	24	35	supplemented	supplement	VERB
cet-12518	24	36	with	with	ADP
cet-12518	24	37	ampicillin	ampicillin	NOUN
cet-12518	24	38	100	100	NUM
cet-12518	24	39	µg	µg	NOUN
cet-12518	24	40	/	/	SYM
cet-12518	24	41	ml	ml	NOUN
cet-12518	24	42	.	.	PUNCT
cet-12518	25	1	the	the	DET
cet-12518	25	2	culture	culture	NOUN
cet-12518	25	3	was	be	AUX
cet-12518	25	4	incubated	incubate	VERB
cet-12518	25	5	at	at	ADP
cet-12518	25	6	37	37	NUM
cet-12518	25	7	°	°	NOUN
cet-12518	25	8	c	c	NOUN
cet-12518	25	9	for	for	ADP
cet-12518	25	10	12	12	NUM
cet-12518	25	11	hours	hour	NOUN
cet-12518	25	12	.	.	PUNCT
cet-12518	26	1	2.2	2.2	NUM
cet-12518	26	2	preinoculum	preinoculum	NOUN
cet-12518	26	3	inoculum	inoculum	NOUN
cet-12518	26	4	was	be	AUX
cet-12518	26	5	inoculated	inoculate	VERB
cet-12518	26	6	in	in	ADP
cet-12518	26	7	a	a	DET
cet-12518	26	8	shake	shake	NOUN
cet-12518	26	9	flask	flask	NOUN
cet-12518	26	10	with	with	ADP
cet-12518	26	11	200	200	NUM
cet-12518	26	12	ml	ml	NOUN
cet-12518	26	13	of	of	ADP
cet-12518	26	14	lb	lb	DET
cet-12518	26	15	-	-	PUNCT
cet-12518	26	16	ampicillim	ampicillim	NOUN
cet-12518	26	17	broth	broth	NOUN
cet-12518	26	18	for	for	ADP
cet-12518	26	19	transformed	transform	VERB
cet-12518	26	20	strain	strain	NOUN
cet-12518	26	21	.	.	PUNCT
cet-12518	27	1	culture	culture	NOUN
cet-12518	27	2	was	be	AUX
cet-12518	27	3	incubated	incubate	VERB
cet-12518	27	4	at	at	ADP
cet-12518	27	5	37	37	NUM
cet-12518	27	6	°	°	SYM
cet-12518	27	7	c	c	NOUN
cet-12518	27	8	at	at	ADP
cet-12518	27	9	200	200	NUM
cet-12518	27	10	rpm	rpm	NOUN
cet-12518	27	11	in	in	ADP
cet-12518	27	12	a	a	DET
cet-12518	27	13	shaker	shaker	NOUN
cet-12518	27	14	incubator	incubator	NOUN
cet-12518	27	15	.	.	PUNCT
cet-12518	28	1	2.3	2.3	NUM
cet-12518	28	2	batch	batch	NOUN
cet-12518	28	3	fermentation	fermentation	NOUN
cet-12518	28	4	condition	condition	NOUN
cet-12518	28	5	and	and	CCONJ
cet-12518	28	6	expression	expression	NOUN
cet-12518	28	7	plasmid	plasmid	VERB
cet-12518	28	8	the	the	DET
cet-12518	28	9	conditions	condition	NOUN
cet-12518	28	10	of	of	ADP
cet-12518	28	11	bioreactor	bioreactor	NOUN
cet-12518	28	12	,	,	PUNCT
cet-12518	28	13	bioflo	bioflo	NOUN
cet-12518	28	14	/	/	SYM
cet-12518	28	15	celligen	celligen	PROPN
cet-12518	28	16	115	115	NUM
cet-12518	28	17	®	®	NOUN
cet-12518	28	18	,	,	PUNCT
cet-12518	28	19	was	be	AUX
cet-12518	28	20	as	as	SCONJ
cet-12518	28	21	follows	follow	VERB
cet-12518	28	22	:	:	PUNCT
cet-12518	28	23	presterilized	presterilize	VERB
cet-12518	28	24	400	400	NUM
cet-12518	28	25	ml	ml	NOUN
cet-12518	28	26	lb	lb	NUM
cet-12518	28	27	-	-	PUNCT
cet-12518	28	28	ampicillim	ampicillim	NOUN
cet-12518	28	29	broth	broth	NOUN
cet-12518	28	30	(	(	PUNCT
cet-12518	28	31	working	working	NOUN
cet-12518	28	32	volume	volume	NOUN
cet-12518	28	33	)	)	PUNCT
cet-12518	28	34	,	,	PUNCT
cet-12518	28	35	1	1	NUM
cet-12518	28	36	%	%	NOUN
cet-12518	28	37	of	of	ADP
cet-12518	28	38	preinoculum	preinoculum	NOUN
cet-12518	28	39	,	,	PUNCT
cet-12518	28	40	temperature	temperature	NOUN
cet-12518	28	41	37	37	NUM
cet-12518	28	42	°	°	SYM
cet-12518	28	43	c	c	NOUN
cet-12518	28	44	,	,	PUNCT
cet-12518	28	45	airflow	airflow	NOUN
cet-12518	28	46	rate	rate	NOUN
cet-12518	28	47	1	1	NUM
cet-12518	28	48	vvm	vvm	NOUN
cet-12518	28	49	(	(	PUNCT
cet-12518	28	50	volume	volume	NOUN
cet-12518	28	51	of	of	ADP
cet-12518	28	52	air	air	NOUN
cet-12518	28	53	per	per	ADP
cet-12518	28	54	unit	unit	NOUN
cet-12518	28	55	of	of	ADP
cet-12518	28	56	growth	growth	NOUN
cet-12518	28	57	medium	medium	NOUN
cet-12518	28	58	per	per	ADP
cet-12518	28	59	minute	minute	NOUN
cet-12518	28	60	)	)	PUNCT
cet-12518	28	61	.	.	PUNCT
cet-12518	29	1	two	two	NUM
cet-12518	29	2	agitation	agitation	NOUN
cet-12518	29	3	rates	rate	NOUN
cet-12518	29	4	(	(	PUNCT
cet-12518	29	5	200	200	NUM
cet-12518	29	6	-350	-350	PROPN
cet-12518	29	7	rpm	rpm	PROPN
cet-12518	29	8	)	)	PUNCT
cet-12518	29	9	were	be	AUX
cet-12518	29	10	evaluated	evaluate	VERB
cet-12518	29	11	.	.	PUNCT
cet-12518	30	1	the	the	DET
cet-12518	30	2	batch	batch	NOUN
cet-12518	30	3	fermentation	fermentation	NOUN
cet-12518	30	4	process	process	NOUN
cet-12518	30	5	continued	continue	VERB
cet-12518	30	6	until	until	SCONJ
cet-12518	30	7	obtain	obtain	VERB
cet-12518	30	8	an	an	DET
cet-12518	30	9	optical	optical	ADJ
cet-12518	30	10	density	density	NOUN
cet-12518	30	11	(	(	PUNCT
cet-12518	30	12	od595	od595	PROPN
cet-12518	30	13	nm	nm	NOUN
cet-12518	30	14	)	)	PUNCT
cet-12518	30	15	between	between	ADP
cet-12518	30	16	0.28	0.28	NUM
cet-12518	30	17	–	–	PUNCT
cet-12518	30	18	0.32	0.32	NUM
cet-12518	30	19	.	.	PUNCT
cet-12518	31	1	the	the	DET
cet-12518	31	2	induction	induction	NOUN
cet-12518	31	3	was	be	AUX
cet-12518	31	4	performed	perform	VERB
cet-12518	31	5	by	by	ADP
cet-12518	31	6	0.5	0.5	NUM
cet-12518	31	7	mm	mm	NOUN
cet-12518	31	8	isopropyl	isopropyl	NOUN
cet-12518	31	9	-	-	PUNCT
cet-12518	31	10	β	β	NOUN
cet-12518	31	11	-	-	PUNCT
cet-12518	31	12	d-1	d-1	NOUN
cet-12518	31	13	-	-	PUNCT
cet-12518	31	14	thiogalactopyranoside	thiogalactopyranoside	NOUN
cet-12518	31	15	(	(	PUNCT
cet-12518	31	16	iptg	iptg	NOUN
cet-12518	31	17	)	)	PUNCT
cet-12518	31	18	and	and	CCONJ
cet-12518	31	19	continued	continue	VERB
cet-12518	31	20	for	for	ADP
cet-12518	31	21	16	16	NUM
cet-12518	31	22	hours	hour	NOUN
cet-12518	31	23	(	(	PUNCT
cet-12518	31	24	singh	singh	PROPN
cet-12518	31	25	et	et	PROPN
cet-12518	31	26	al	al	PROPN
cet-12518	31	27	.	.	PROPN
cet-12518	31	28	,	,	PUNCT
cet-12518	31	29	2015	2015	NUM
cet-12518	31	30	)	)	PUNCT
cet-12518	31	31	.	.	PUNCT
cet-12518	32	1	the	the	DET
cet-12518	32	2	experiments	experiment	NOUN
cet-12518	32	3	were	be	AUX
cet-12518	32	4	performed	perform	VERB
cet-12518	32	5	by	by	ADP
cet-12518	32	6	duplicate	duplicate	NOUN
cet-12518	32	7	for	for	ADP
cet-12518	32	8	two	two	NUM
cet-12518	32	9	agitation	agitation	NOUN
cet-12518	32	10	speeds	speed	NOUN
cet-12518	32	11	.	.	PUNCT
cet-12518	33	1	2.4	2.4	NUM
cet-12518	33	2	biomass	biomass	NOUN
cet-12518	33	3	determination	determination	NOUN
cet-12518	33	4	biomass	biomass	NOUN
cet-12518	33	5	was	be	AUX
cet-12518	33	6	determined	determine	VERB
cet-12518	33	7	by	by	ADP
cet-12518	33	8	optical	optical	ADJ
cet-12518	33	9	density	density	NOUN
cet-12518	33	10	at	at	ADP
cet-12518	33	11	595	595	NUM
cet-12518	33	12	nm	nm	NOUN
cet-12518	33	13	(	(	PUNCT
cet-12518	33	14	od595	od595	NOUN
cet-12518	33	15	nm	nm	PROPN
cet-12518	33	16	)	)	PUNCT
cet-12518	33	17	,	,	PUNCT
cet-12518	33	18	from	from	ADP
cet-12518	33	19	aliquots	aliquot	NOUN
cet-12518	33	20	of	of	ADP
cet-12518	33	21	fermentation	fermentation	NOUN
cet-12518	33	22	broth	broth	NOUN
cet-12518	33	23	,	,	PUNCT
cet-12518	33	24	was	be	AUX
cet-12518	33	25	collected	collect	VERB
cet-12518	33	26	at	at	ADP
cet-12518	33	27	every	every	DET
cet-12518	33	28	60	60	NUM
cet-12518	33	29	minutes	minute	NOUN
cet-12518	33	30	during	during	ADP
cet-12518	33	31	the	the	DET
cet-12518	33	32	first	first	ADJ
cet-12518	33	33	9	9	NUM
cet-12518	33	34	hours	hour	NOUN
cet-12518	33	35	of	of	ADP
cet-12518	33	36	fermentation	fermentation	NOUN
cet-12518	33	37	process	process	NOUN
cet-12518	33	38	(	(	PUNCT
cet-12518	33	39	before	before	ADP
cet-12518	33	40	and	and	CCONJ
cet-12518	33	41	after	after	ADP
cet-12518	33	42	induction	induction	NOUN
cet-12518	33	43	stage	stage	NOUN
cet-12518	33	44	)	)	PUNCT
cet-12518	33	45	.	.	PUNCT
cet-12518	34	1	2.5	2.5	NUM
cet-12518	34	2	extraction	extraction	NOUN
cet-12518	34	3	and	and	CCONJ
cet-12518	34	4	purification	purification	NOUN
cet-12518	34	5	of	of	ADP
cet-12518	34	6	taq	taq	NOUN
cet-12518	34	7	polymerase	polymerase	NOUN
cet-12518	34	8	the	the	DET
cet-12518	34	9	fermented	ferment	VERB
cet-12518	34	10	broth	broth	NOUN
cet-12518	34	11	was	be	AUX
cet-12518	34	12	recovered	recover	VERB
cet-12518	34	13	in	in	ADP
cet-12518	34	14	centrifuge	centrifuge	NOUN
cet-12518	34	15	tubes	tube	NOUN
cet-12518	34	16	,	,	PUNCT
cet-12518	34	17	they	they	PRON
cet-12518	34	18	were	be	AUX
cet-12518	34	19	spun	spin	VERB
cet-12518	34	20	at	at	ADP
cet-12518	34	21	4800	4800	NUM
cet-12518	34	22	rpm	rpm	NOUN
cet-12518	34	23	during	during	ADP
cet-12518	34	24	20	20	NUM
cet-12518	34	25	minutes	minute	NOUN
cet-12518	34	26	at	at	ADP
cet-12518	34	27	4	4	NUM
cet-12518	34	28	°	°	NOUN
cet-12518	34	29	c	c	NOUN
cet-12518	34	30	.	.	PUNCT
cet-12518	35	1	the	the	DET
cet-12518	35	2	fermented	ferment	VERB
cet-12518	35	3	cell	cell	NOUN
cet-12518	35	4	washed	wash	VERB
cet-12518	35	5	pellet	pellet	NOUN
cet-12518	35	6	was	be	AUX
cet-12518	35	7	suspended	suspend	VERB
cet-12518	35	8	with	with	ADP
cet-12518	35	9	30	30	NUM
cet-12518	35	10	ml	ml	NOUN
cet-12518	35	11	pre	pre	ADJ
cet-12518	35	12	-	-	ADJ
cet-12518	35	13	cell	cell	ADJ
cet-12518	35	14	lysis	lysis	NOUN
cet-12518	35	15	buffer	buffer	NOUN
cet-12518	35	16	(	(	PUNCT
cet-12518	35	17	50	50	NUM
cet-12518	35	18	mm	mm	NOUN
cet-12518	35	19	tris	tris	NOUN
cet-12518	35	20	-	-	PUNCT
cet-12518	35	21	hcl	hcl	NOUN
cet-12518	35	22	,	,	PUNCT
cet-12518	35	23	50	50	NUM
cet-12518	35	24	mm	mm	NOUN
cet-12518	35	25	dextrose	dextrose	NOUN
cet-12518	35	26	,	,	PUNCT
cet-12518	35	27	1	1	NUM
cet-12518	35	28	mm	mm	NOUN
cet-12518	35	29	edta	edta	NOUN
cet-12518	35	30	and	and	CCONJ
cet-12518	35	31	100	100	NUM
cet-12518	35	32	µg	µg	ADJ
cet-12518	35	33	/	/	SYM
cet-12518	35	34	ml	ml	NOUN
cet-12518	35	35	of	of	ADP
cet-12518	35	36	lysozyme	lysozyme	NOUN
cet-12518	35	37	)	)	PUNCT
cet-12518	35	38	and	and	CCONJ
cet-12518	35	39	agitated	agitate	VERB
cet-12518	35	40	by	by	ADP
cet-12518	35	41	vortex	vortex	NOUN
cet-12518	35	42	(	(	PUNCT
cet-12518	35	43	ferralli	ferralli	PROPN
cet-12518	35	44	et	et	PROPN
cet-12518	35	45	al	al	PROPN
cet-12518	35	46	.	.	PROPN
cet-12518	35	47	,	,	PUNCT
cet-12518	35	48	2007	2007	NUM
cet-12518	35	49	)	)	PUNCT
cet-12518	35	50	.	.	PUNCT
cet-12518	36	1	the	the	DET
cet-12518	36	2	supernatant	supernatant	NOUN
cet-12518	36	3	was	be	AUX
cet-12518	36	4	incubated	incubate	VERB
cet-12518	36	5	at	at	ADP
cet-12518	36	6	37	37	NUM
cet-12518	36	7	°	°	NOUN
cet-12518	36	8	c	c	NOUN
cet-12518	36	9	for	for	ADP
cet-12518	36	10	30	30	NUM
cet-12518	36	11	minutes	minute	NOUN
cet-12518	36	12	and	and	CCONJ
cet-12518	36	13	suspended	suspend	VERB
cet-12518	36	14	in	in	ADP
cet-12518	36	15	cell	cell	NOUN
cet-12518	36	16	lysis	lysis	NOUN
cet-12518	36	17	buffer	buffer	NOUN
cet-12518	36	18	(	(	PUNCT
cet-12518	36	19	50	50	NUM
cet-12518	36	20	mm	mm	NOUN
cet-12518	36	21	tris	tris	NOUN
cet-12518	36	22	-	-	PUNCT
cet-12518	36	23	hcl	hcl	NOUN
cet-12518	36	24	,	,	PUNCT
cet-12518	36	25	50	50	NUM
cet-12518	36	26	mm	mm	NOUN
cet-12518	36	27	kcl	kcl	NOUN
cet-12518	36	28	,	,	PUNCT
cet-12518	36	29	1	1	NUM
cet-12518	36	30	mm	mm	NUM
cet-12518	36	31	edta	edta	NOUN
cet-12518	36	32	,	,	PUNCT
cet-12518	36	33	1	1	NUM
cet-12518	36	34	%	%	NOUN
cet-12518	36	35	triton	triton	PROPN
cet-12518	36	36	x-100	x-100	PROPN
cet-12518	36	37	and	and	CCONJ
cet-12518	36	38	pmsf	pmsf	NOUN
cet-12518	36	39	)	)	PUNCT
cet-12518	36	40	(	(	PUNCT
cet-12518	36	41	farazmandfar	farazmandfar	ADV
cet-12518	36	42	et	et	PROPN
cet-12518	36	43	al	al	PROPN
cet-12518	36	44	,	,	PUNCT
cet-12518	36	45	2013	2013	NUM
cet-12518	36	46	)	)	PUNCT
cet-12518	37	1	it	it	PRON
cet-12518	37	2	was	be	AUX
cet-12518	37	3	agitated	agitate	VERB
cet-12518	37	4	by	by	ADP
cet-12518	37	5	vortex	vortex	NOUN
cet-12518	37	6	and	and	CCONJ
cet-12518	37	7	incubated	incubate	VERB
cet-12518	37	8	in	in	ADP
cet-12518	37	9	a	a	DET
cet-12518	37	10	water	water	NOUN
cet-12518	37	11	bath	bath	NOUN
cet-12518	37	12	at	at	ADP
cet-12518	37	13	75	75	NUM
cet-12518	37	14	°	°	NUM
cet-12518	37	15	c	c	NOUN
cet-12518	37	16	for	for	ADP
cet-12518	37	17	an	an	DET
cet-12518	37	18	hour	hour	NOUN
cet-12518	37	19	.	.	PUNCT
cet-12518	38	1	protein	protein	NOUN
cet-12518	38	2	was	be	AUX
cet-12518	38	3	obtained	obtain	VERB
cet-12518	38	4	by	by	ADP
cet-12518	38	5	centrifuging	centrifuge	VERB
cet-12518	38	6	at	at	ADP
cet-12518	38	7	10000	10000	NUM
cet-12518	38	8	rpm	rpm	NOUN
cet-12518	38	9	for	for	ADP
cet-12518	38	10	15	15	NUM
cet-12518	38	11	minutes	minute	NOUN
cet-12518	38	12	at	at	ADP
cet-12518	38	13	4	4	NUM
cet-12518	38	14	°	°	NOUN
cet-12518	38	15	c	c	NOUN
cet-12518	38	16	.	.	NOUN
cet-12518	38	17	2.6	2.6	NUM
cet-12518	38	18	protein	protein	NOUN
cet-12518	38	19	determination	determination	NOUN
cet-12518	38	20	and	and	CCONJ
cet-12518	38	21	quantification	quantification	NOUN
cet-12518	38	22	protein	protein	NOUN
cet-12518	38	23	concentration	concentration	NOUN
cet-12518	38	24	was	be	AUX
cet-12518	38	25	determined	determine	VERB
cet-12518	38	26	according	accord	VERB
cet-12518	38	27	to	to	ADP
cet-12518	38	28	bradford	bradford	PROPN
cet-12518	38	29	(	(	PUNCT
cet-12518	38	30	bradford	bradford	NOUN
cet-12518	38	31	,	,	PUNCT
cet-12518	38	32	1976	1976	NUM
cet-12518	38	33	)	)	PUNCT
cet-12518	38	34	using	use	VERB
cet-12518	38	35	gammaglobuline	gammaglobuline	NOUN
cet-12518	38	36	at	at	ADP
cet-12518	38	37	640	640	NUM
cet-12518	38	38	µg	µg	NOUN
cet-12518	38	39	/	/	SYM
cet-12518	38	40	ml	ml	NOUN
cet-12518	38	41	as	as	ADP
cet-12518	38	42	patron	patron	NOUN
cet-12518	38	43	curve	curve	NOUN
cet-12518	38	44	.	.	PUNCT
cet-12518	39	1	coomassie	coomassie	PROPN
cet-12518	39	2	blue	blue	PROPN
cet-12518	39	3	g-250	g-250	PROPN
cet-12518	39	4	was	be	AUX
cet-12518	39	5	used	use	VERB
cet-12518	39	6	for	for	ADP
cet-12518	39	7	protein	protein	NOUN
cet-12518	39	8	visualization	visualization	NOUN
cet-12518	39	9	.	.	PUNCT
cet-12518	40	1	gel	gel	NOUN
cet-12518	40	2	electrophoresis	electrophoresis	NOUN
cet-12518	40	3	was	be	AUX
cet-12518	40	4	used	use	VERB
cet-12518	40	5	to	to	PART
cet-12518	40	6	analyze	analyze	VERB
cet-12518	40	7	protein	protein	NOUN
cet-12518	40	8	(	(	PUNCT
cet-12518	40	9	biorad	biorad	ADJ
cet-12518	40	10	,	,	PUNCT
cet-12518	40	11	2020	2020	NUM
cet-12518	40	12	)	)	PUNCT
cet-12518	40	13	and	and	CCONJ
cet-12518	40	14	visualization	visualization	NOUN
cet-12518	40	15	was	be	AUX
cet-12518	40	16	made	make	VERB
cet-12518	40	17	by	by	ADP
cet-12518	40	18	coomassie	coomassie	ADJ
cet-12518	40	19	blue	blue	NOUN
cet-12518	40	20	and	and	CCONJ
cet-12518	40	21	a	a	DET
cet-12518	40	22	molecular	molecular	ADJ
cet-12518	40	23	weight	weight	NOUN
cet-12518	40	24	tracer	tracer	NOUN
cet-12518	40	25	with	with	ADP
cet-12518	40	26	a	a	DET
cet-12518	40	27	range	range	NOUN
cet-12518	40	28	of	of	ADP
cet-12518	40	29	11	11	NUM
cet-12518	40	30	kda	kda	NOUN
cet-12518	40	31	to	to	ADP
cet-12518	40	32	245	245	NUM
cet-12518	40	33	kda	kda	NOUN
cet-12518	40	34	.	.	PUNCT
cet-12518	41	1	each	each	DET
cet-12518	41	2	agitation	agitation	NOUN
cet-12518	41	3	level	level	NOUN
cet-12518	41	4	was	be	AUX
cet-12518	41	5	measured	measure	VERB
cet-12518	41	6	by	by	ADP
cet-12518	41	7	duplicate	duplicate	NOUN
cet-12518	41	8	.	.	PUNCT
cet-12518	42	1	2.7	2.7	NUM
cet-12518	42	2	measurement	measurement	NOUN
cet-12518	42	3	of	of	ADP
cet-12518	42	4	operation	operation	NOUN
cet-12518	42	5	parameters	parameter	NOUN
cet-12518	42	6	dissolved	dissolve	VERB
cet-12518	42	7	oxygen	oxygen	NOUN
cet-12518	42	8	and	and	CCONJ
cet-12518	42	9	ph	ph	NOUN
cet-12518	42	10	were	be	AUX
cet-12518	42	11	monitored	monitor	VERB
cet-12518	42	12	by	by	ADP
cet-12518	42	13	means	mean	NOUN
cet-12518	42	14	of	of	ADP
cet-12518	42	15	software	software	NOUN
cet-12518	42	16	biocommand	biocommand	NOUN
cet-12518	42	17	®	®	NOUN
cet-12518	42	18	and	and	CCONJ
cet-12518	42	19	the	the	DET
cet-12518	42	20	respective	respective	ADJ
cet-12518	42	21	electrodes	electrode	NOUN
cet-12518	42	22	for	for	ADP
cet-12518	42	23	all	all	DET
cet-12518	42	24	the	the	DET
cet-12518	42	25	fermentation	fermentation	NOUN
cet-12518	42	26	process	process	NOUN
cet-12518	42	27	(	(	PUNCT
cet-12518	42	28	21	21	NUM
cet-12518	42	29	hours	hour	NOUN
cet-12518	42	30	)	)	PUNCT
cet-12518	42	31	,	,	PUNCT
cet-12518	42	32	giving	give	VERB
cet-12518	42	33	21	21	NUM
cet-12518	42	34	data	datum	NOUN
cet-12518	42	35	or	or	CCONJ
cet-12518	42	36	each	each	DET
cet-12518	42	37	replica	replica	NOUN
cet-12518	42	38	in	in	ADP
cet-12518	42	39	each	each	PRON
cet-12518	42	40	of	of	ADP
cet-12518	42	41	the	the	DET
cet-12518	42	42	agitation	agitation	NOUN
cet-12518	42	43	level	level	NOUN
cet-12518	42	44	.	.	PUNCT
cet-12518	43	1	3	3	X
cet-12518	43	2	.	.	NOUN
cet-12518	43	3	results	result	NOUN
cet-12518	43	4	and	and	CCONJ
cet-12518	43	5	discussion	discussion	NOUN
cet-12518	43	6	3.1	3.1	NUM
cet-12518	43	7	biomass	biomass	NOUN
cet-12518	43	8	determination	determination	NOUN
cet-12518	43	9	the	the	DET
cet-12518	43	10	indirect	indirect	ADJ
cet-12518	43	11	measurement	measurement	NOUN
cet-12518	43	12	of	of	ADP
cet-12518	43	13	cell	cell	NOUN
cet-12518	43	14	growth	growth	NOUN
cet-12518	43	15	by	by	ADP
cet-12518	43	16	optical	optical	ADJ
cet-12518	43	17	density	density	NOUN
cet-12518	43	18	was	be	AUX
cet-12518	43	19	performed	perform	VERB
cet-12518	43	20	during	during	ADP
cet-12518	43	21	the	the	DET
cet-12518	43	22	first	first	ADJ
cet-12518	43	23	9	9	NUM
cet-12518	43	24	hours	hour	NOUN
cet-12518	43	25	.	.	PUNCT
cet-12518	44	1	in	in	ADP
cet-12518	44	2	the	the	DET
cet-12518	44	3	first	first	ADJ
cet-12518	44	4	4	4	NUM
cet-12518	44	5	hours	hour	NOUN
cet-12518	44	6	occurs	occur	VERB
cet-12518	44	7	latent	latent	NOUN
cet-12518	44	8	phase	phase	NOUN
cet-12518	44	9	and	and	CCONJ
cet-12518	44	10	the	the	DET
cet-12518	44	11	following	follow	VERB
cet-12518	44	12	hours	hour	NOUN
cet-12518	44	13	the	the	DET
cet-12518	44	14	exponential	exponential	ADJ
cet-12518	44	15	phase	phase	NOUN
cet-12518	44	16	.	.	PUNCT
cet-12518	45	1	about	about	ADV
cet-12518	45	2	the	the	DET
cet-12518	45	3	fifth	fifth	ADJ
cet-12518	45	4	hour	hour	NOUN
cet-12518	45	5	of	of	ADP
cet-12518	45	6	incubation	incubation	NOUN
cet-12518	45	7	in	in	ADP
cet-12518	45	8	which	which	PRON
cet-12518	45	9	the	the	DET
cet-12518	45	10	od	od	NOUN
cet-12518	45	11	was	be	AUX
cet-12518	45	12	in	in	ADP
cet-12518	45	13	the	the	DET
cet-12518	45	14	range	range	NOUN
cet-12518	45	15	of	of	ADP
cet-12518	45	16	0.28	0.28	NUM
cet-12518	45	17	0.32	0.32	NUM
cet-12518	45	18	,	,	PUNCT
cet-12518	45	19	the	the	DET
cet-12518	45	20	induction	induction	NOUN
cet-12518	45	21	process	process	NOUN
cet-12518	45	22	was	be	AUX
cet-12518	45	23	carried	carry	VERB
cet-12518	45	24	out	out	ADP
cet-12518	45	25	with	with	ADP
cet-12518	45	26	0.5	0.5	NUM
cet-12518	45	27	mm	mm	NOUN
cet-12518	45	28	of	of	ADP
cet-12518	45	29	iptg	iptg	PROPN
cet-12518	45	30	.	.	PUNCT
cet-12518	46	1	figures	figure	NOUN
cet-12518	46	2	1	1	NUM
cet-12518	46	3	show	show	VERB
cet-12518	46	4	that	that	SCONJ
cet-12518	46	5	cell	cell	NOUN
cet-12518	46	6	growth	growth	NOUN
cet-12518	46	7	is	be	AUX
cet-12518	46	8	similar	similar	ADJ
cet-12518	46	9	at	at	ADP
cet-12518	46	10	both	both	DET
cet-12518	46	11	levels	level	NOUN
cet-12518	46	12	of	of	ADP
cet-12518	46	13	agitation	agitation	NOUN
cet-12518	46	14	,	,	PUNCT
cet-12518	46	15	however	however	ADV
cet-12518	46	16	,	,	PUNCT
cet-12518	46	17	in	in	ADP
cet-12518	46	18	the	the	DET
cet-12518	46	19	agitation	agitation	NOUN
cet-12518	46	20	at	at	ADP
cet-12518	46	21	350	350	NUM
cet-12518	46	22	rpm	rpm	NOUN
cet-12518	46	23	there	there	PRON
cet-12518	46	24	was	be	VERB
cet-12518	46	25	a	a	DET
cet-12518	46	26	slight	slight	ADJ
cet-12518	46	27	increase	increase	NOUN
cet-12518	46	28	over	over	ADP
cet-12518	46	29	the	the	DET
cet-12518	46	30	last	last	ADJ
cet-12518	46	31	few	few	ADJ
cet-12518	46	32	hours	hour	NOUN
cet-12518	46	33	evaluate	evaluate	VERB
cet-12518	46	34	.	.	PUNCT
cet-12518	47	1	the	the	DET
cet-12518	47	2	monitoring	monitoring	NOUN
cet-12518	47	3	of	of	ADP
cet-12518	47	4	microbial	microbial	ADJ
cet-12518	47	5	growth	growth	NOUN
cet-12518	47	6	and	and	CCONJ
cet-12518	47	7	its	its	PRON
cet-12518	47	8	physiology	physiology	NOUN
cet-12518	47	9	allow	allow	VERB
cet-12518	47	10	to	to	PART
cet-12518	47	11	infer	infer	VERB
cet-12518	47	12	that	that	SCONJ
cet-12518	47	13	the	the	DET
cet-12518	47	14	cells	cell	NOUN
cet-12518	47	15	did	do	AUX
cet-12518	47	16	not	not	PART
cet-12518	47	17	present	present	VERB
cet-12518	47	18	stress	stress	NOUN
cet-12518	47	19	when	when	SCONJ
cet-12518	47	20	changing	change	VERB
cet-12518	47	21	from	from	ADP
cet-12518	47	22	an	an	DET
cet-12518	47	23	orbital	orbital	ADJ
cet-12518	47	24	shaking	shake	VERB
cet-12518	47	25	agitation	agitation	NOUN
cet-12518	47	26	to	to	ADP
cet-12518	47	27	a	a	DET
cet-12518	47	28	continued	continue	VERB
cet-12518	47	29	stirred	stir	VERB
cet-12518	47	30	reactor	reactor	NOUN
cet-12518	47	31	,	,	PUNCT
cet-12518	47	32	this	this	DET
cet-12518	47	33	system	system	NOUN
cet-12518	47	34	may	may	AUX
cet-12518	47	35	favour	favour	VERB
cet-12518	47	36	oxygen	oxygen	NOUN
cet-12518	47	37	mixing	mixing	NOUN
cet-12518	47	38	and	and	CCONJ
cet-12518	47	39	transfer	transfer	NOUN
cet-12518	47	40	processes	process	NOUN
cet-12518	47	41	which	which	PRON
cet-12518	47	42	may	may	AUX
cet-12518	47	43	influence	influence	VERB
cet-12518	47	44	cell	cell	NOUN
cet-12518	47	45	integrity	integrity	NOUN
cet-12518	47	46	122	122	NUM
cet-12518	47	47	and	and	CCONJ
cet-12518	47	48	increase	increase	VERB
cet-12518	47	49	in	in	ADP
cet-12518	47	50	protein	protein	NOUN
cet-12518	47	51	production	production	NOUN
cet-12518	47	52	(	(	PUNCT
cet-12518	47	53	bagagli	bagagli	NOUN
cet-12518	47	54	and	and	CCONJ
cet-12518	47	55	sato	sato	NOUN
cet-12518	47	56	2013	2013	NUM
cet-12518	47	57	)	)	PUNCT
cet-12518	47	58	.	.	PUNCT
cet-12518	48	1	these	these	DET
cet-12518	48	2	results	result	NOUN
cet-12518	48	3	can	can	AUX
cet-12518	48	4	be	be	AUX
cet-12518	48	5	a	a	DET
cet-12518	48	6	first	first	ADJ
cet-12518	48	7	approximation	approximation	NOUN
cet-12518	48	8	,	,	PUNCT
cet-12518	48	9	considering	consider	VERB
cet-12518	48	10	that	that	SCONJ
cet-12518	48	11	the	the	DET
cet-12518	48	12	relation	relation	NOUN
cet-12518	48	13	between	between	ADP
cet-12518	48	14	aeration	aeration	NOUN
cet-12518	48	15	rate	rate	NOUN
cet-12518	48	16	and	and	CCONJ
cet-12518	48	17	impeller	impeller	NOUN
cet-12518	48	18	agitation	agitation	NOUN
cet-12518	48	19	speed	speed	NOUN
cet-12518	48	20	is	be	AUX
cet-12518	48	21	so	so	ADV
cet-12518	48	22	important	important	ADJ
cet-12518	48	23	as	as	ADP
cet-12518	48	24	a	a	DET
cet-12518	48	25	scale	scale	NOUN
cet-12518	48	26	-	-	PUNCT
cet-12518	48	27	up	up	ADP
cet-12518	48	28	parameter	parameter	NOUN
cet-12518	48	29	in	in	ADP
cet-12518	48	30	the	the	DET
cet-12518	48	31	protein	protein	NOUN
cet-12518	48	32	production	production	NOUN
cet-12518	48	33	by	by	ADP
cet-12518	48	34	e.coli	e.coli	NOUN
cet-12518	48	35	as	as	ADP
cet-12518	48	36	a	a	DET
cet-12518	48	37	host	host	NOUN
cet-12518	48	38	microorganism	microorganism	NOUN
cet-12518	48	39	(	(	PUNCT
cet-12518	48	40	espinosa	espinosa	PROPN
cet-12518	48	41	et	et	PROPN
cet-12518	48	42	al	al	PROPN
cet-12518	48	43	.	.	PROPN
cet-12518	48	44	,	,	PUNCT
cet-12518	48	45	2018	2018	NUM
cet-12518	48	46	)	)	PUNCT
cet-12518	48	47	.	.	PUNCT
cet-12518	49	1	figure	figure	VERB
cet-12518	49	2	1	1	NUM
cet-12518	49	3	:	:	PUNCT
cet-12518	49	4	biomass	biomass	NOUN
cet-12518	49	5	analysis	analysis	NOUN
cet-12518	49	6	at	at	ADP
cet-12518	49	7	different	different	ADJ
cet-12518	49	8	agitation	agitation	NOUN
cet-12518	49	9	speeds	speed	VERB
cet-12518	49	10	a	a	PRON
cet-12518	49	11	)	)	PUNCT
cet-12518	49	12	220	220	NUM
cet-12518	49	13	rpm	rpm	NOUN
cet-12518	49	14	,	,	PUNCT
cet-12518	49	15	b	b	NOUN
cet-12518	49	16	)	)	PUNCT
cet-12518	49	17	350	350	NUM
cet-12518	49	18	rpm	rpm	NOUN
cet-12518	49	19	3.2	3.2	NUM
cet-12518	49	20	protein	protein	NOUN
cet-12518	49	21	determination	determination	NOUN
cet-12518	49	22	and	and	CCONJ
cet-12518	49	23	quantification	quantification	NOUN
cet-12518	49	24	as	as	ADP
cet-12518	49	25	table	table	NOUN
cet-12518	49	26	1	1	NUM
cet-12518	49	27	shows	show	NOUN
cet-12518	49	28	,	,	PUNCT
cet-12518	49	29	at	at	ADP
cet-12518	49	30	350	350	NUM
cet-12518	49	31	rpm	rpm	NOUN
cet-12518	49	32	there	there	PRON
cet-12518	49	33	is	be	VERB
cet-12518	49	34	a	a	DET
cet-12518	49	35	higher	high	ADJ
cet-12518	49	36	production	production	NOUN
cet-12518	49	37	of	of	ADP
cet-12518	49	38	protein	protein	NOUN
cet-12518	49	39	than	than	ADP
cet-12518	49	40	at	at	ADP
cet-12518	49	41	220	220	NUM
cet-12518	49	42	rpm	rpm	NOUN
cet-12518	49	43	,	,	PUNCT
cet-12518	49	44	showing	show	VERB
cet-12518	49	45	that	that	SCONJ
cet-12518	49	46	the	the	DET
cet-12518	49	47	aeration	aeration	NOUN
cet-12518	49	48	and	and	CCONJ
cet-12518	49	49	mixing	mixing	NOUN
cet-12518	49	50	process	process	NOUN
cet-12518	49	51	is	be	AUX
cet-12518	49	52	more	more	ADV
cet-12518	49	53	effective	effective	ADJ
cet-12518	49	54	,	,	PUNCT
cet-12518	49	55	managing	manage	VERB
cet-12518	49	56	to	to	PART
cet-12518	49	57	better	well	ADV
cet-12518	49	58	supply	supply	VERB
cet-12518	49	59	the	the	DET
cet-12518	49	60	respiratory	respiratory	ADJ
cet-12518	49	61	and	and	CCONJ
cet-12518	49	62	energy	energy	NOUN
cet-12518	49	63	demand	demand	NOUN
cet-12518	49	64	required	require	VERB
cet-12518	49	65	for	for	ADP
cet-12518	49	66	the	the	DET
cet-12518	49	67	metabolic	metabolic	NOUN
cet-12518	49	68	processes	process	NOUN
cet-12518	49	69	of	of	ADP
cet-12518	49	70	e.	e.	PROPN
cet-12518	49	71	coli	coli	PROPN
cet-12518	49	72	,	,	PUNCT
cet-12518	49	73	biosynthetic	biosynthetic	ADJ
cet-12518	49	74	processes	process	NOUN
cet-12518	49	75	require	require	VERB
cet-12518	49	76	large	large	ADJ
cet-12518	49	77	dell	dell	NOUN
cet-12518	49	78	energy	energy	NOUN
cet-12518	49	79	investments	investment	NOUN
cet-12518	49	80	therefore	therefore	ADV
cet-12518	49	81	oxidative	oxidative	ADJ
cet-12518	49	82	metabolism	metabolism	NOUN
cet-12518	49	83	should	should	AUX
cet-12518	49	84	not	not	PART
cet-12518	49	85	be	be	AUX
cet-12518	49	86	limited	limit	VERB
cet-12518	49	87	to	to	PART
cet-12518	49	88	supply	supply	VERB
cet-12518	49	89	these	these	DET
cet-12518	49	90	needs	need	NOUN
cet-12518	49	91	.	.	PUNCT
cet-12518	50	1	tan	tan	VERB
cet-12518	50	2	et	et	PROPN
cet-12518	50	3	al	al	PROPN
cet-12518	50	4	.	.	PROPN
cet-12518	50	5	,	,	PUNCT
cet-12518	50	6	(	(	PUNCT
cet-12518	50	7	2018	2018	NUM
cet-12518	50	8	)	)	PUNCT
cet-12518	50	9	analyzed	analyze	VERB
cet-12518	50	10	the	the	DET
cet-12518	50	11	influence	influence	NOUN
cet-12518	50	12	of	of	ADP
cet-12518	50	13	agitation	agitation	NOUN
cet-12518	50	14	speed	speed	NOUN
cet-12518	50	15	on	on	ADP
cet-12518	50	16	production	production	NOUN
cet-12518	50	17	of	of	ADP
cet-12518	50	18	a	a	DET
cet-12518	50	19	protein	protein	NOUN
cet-12518	50	20	with	with	ADP
cet-12518	50	21	a	a	DET
cet-12518	50	22	recombinant	recombinant	ADJ
cet-12518	50	23	e.coli	e.coli	NOUN
cet-12518	50	24	de3	de3	NOUN
cet-12518	50	25	,	,	PUNCT
cet-12518	50	26	they	they	PRON
cet-12518	50	27	observed	observe	VERB
cet-12518	50	28	the	the	DET
cet-12518	50	29	highest	high	ADJ
cet-12518	50	30	growth	growth	NOUN
cet-12518	50	31	and	and	CCONJ
cet-12518	50	32	production	production	NOUN
cet-12518	50	33	were	be	AUX
cet-12518	50	34	at	at	ADP
cet-12518	50	35	an	an	DET
cet-12518	50	36	agitation	agitation	NOUN
cet-12518	50	37	speed	speed	NOUN
cet-12518	50	38	of	of	ADP
cet-12518	50	39	1000	1000	NUM
cet-12518	50	40	rpm	rpm	NOUN
cet-12518	50	41	compared	compare	VERB
cet-12518	50	42	whit	whit	NOUN
cet-12518	50	43	less	less	ADJ
cet-12518	50	44	speeds	speed	NOUN
cet-12518	50	45	.	.	PUNCT
cet-12518	51	1	table	table	NOUN
cet-12518	51	2	1	1	NUM
cet-12518	51	3	:	:	PUNCT
cet-12518	51	4	protein	protein	NOUN
cet-12518	51	5	concentration	concentration	NOUN
cet-12518	51	6	1non	1non	NUM
cet-12518	51	7	induced	induce	VERB
cet-12518	51	8	concentration	concentration	NOUN
cet-12518	51	9	;	;	PUNCT
cet-12518	51	10	2average	2average	NUM
cet-12518	51	11	non	non	NOUN
cet-12518	51	12	induced	induced	ADJ
cet-12518	51	13	concentration	concentration	NOUN
cet-12518	51	14	;	;	PUNCT
cet-12518	51	15	3	3	NUM
cet-12518	51	16	induced	induced	ADJ
cet-12518	51	17	concentrations	concentration	NOUN
cet-12518	51	18	;	;	PUNCT
cet-12518	51	19	4	4	NUM
cet-12518	51	20	average	average	ADJ
cet-12518	51	21	induced	induced	ADJ
cet-12518	51	22	concentration	concentration	NOUN
cet-12518	51	23	experimental	experimental	ADJ
cet-12518	51	24	production	production	NOUN
cet-12518	51	25	of	of	ADP
cet-12518	51	26	the	the	DET
cet-12518	51	27	tucan	tucan	PROPN
cet-12518	51	28	taq	taq	PROPN
cet-12518	51	29	®	®	NOUN
cet-12518	51	30	was	be	AUX
cet-12518	51	31	carried	carry	VERB
cet-12518	51	32	out	out	ADP
cet-12518	51	33	at	at	ADP
cet-12518	51	34	210rpm	210rpm	NUM
cet-12518	51	35	with	with	ADP
cet-12518	51	36	orbital	orbital	ADJ
cet-12518	51	37	agitation	agitation	NOUN
cet-12518	51	38	with	with	ADP
cet-12518	51	39	an	an	DET
cet-12518	51	40	average	average	ADJ
cet-12518	51	41	final	final	ADJ
cet-12518	51	42	enzyme	enzyme	NOUN
cet-12518	51	43	production	production	NOUN
cet-12518	51	44	of	of	ADP
cet-12518	51	45	1633	1633	NUM
cet-12518	51	46	µg	µg	NOUN
cet-12518	51	47	/	/	SYM
cet-12518	51	48	ml	ml	PROPN
cet-12518	51	49	(	(	PUNCT
cet-12518	51	50	data	datum	NOUN
cet-12518	51	51	provided	provide	VERB
cet-12518	51	52	by	by	ADP
cet-12518	51	53	corpogen	corpogen	NOUN
cet-12518	51	54	)	)	PUNCT
cet-12518	51	55	.	.	PUNCT
cet-12518	52	1	in	in	ADP
cet-12518	52	2	relation	relation	NOUN
cet-12518	52	3	to	to	ADP
cet-12518	52	4	the	the	DET
cet-12518	52	5	results	result	NOUN
cet-12518	52	6	in	in	ADP
cet-12518	52	7	table	table	NOUN
cet-12518	52	8	1	1	NUM
cet-12518	52	9	and	and	CCONJ
cet-12518	52	10	table	table	NOUN
cet-12518	52	11	2	2	NUM
cet-12518	52	12	,	,	PUNCT
cet-12518	52	13	bioreactor	bioreactor	NOUN
cet-12518	52	14	production	production	NOUN
cet-12518	52	15	with	with	ADP
cet-12518	52	16	a	a	DET
cet-12518	52	17	mechanical	mechanical	ADJ
cet-12518	52	18	stirring	stirring	NOUN
cet-12518	52	19	system	system	NOUN
cet-12518	52	20	at	at	ADP
cet-12518	52	21	350	350	NUM
cet-12518	52	22	rpm	rpm	NOUN
cet-12518	52	23	improved	improve	VERB
cet-12518	52	24	by	by	ADP
cet-12518	52	25	82	82	NUM
cet-12518	52	26	%	%	NOUN
cet-12518	52	27	compared	compare	VERB
cet-12518	52	28	to	to	ADP
cet-12518	52	29	flask	flask	NOUN
cet-12518	52	30	-	-	PUNCT
cet-12518	52	31	level	level	NOUN
cet-12518	52	32	production	production	NOUN
cet-12518	52	33	.	.	PUNCT
cet-12518	53	1	however	however	ADV
cet-12518	53	2	,	,	PUNCT
cet-12518	53	3	at	at	ADP
cet-12518	53	4	an	an	DET
cet-12518	53	5	agitation	agitation	NOUN
cet-12518	53	6	speed	speed	NOUN
cet-12518	53	7	of	of	ADP
cet-12518	53	8	250	250	NUM
cet-12518	53	9	rpm	rpm	NOUN
cet-12518	53	10	the	the	DET
cet-12518	53	11	production	production	NOUN
cet-12518	53	12	was	be	AUX
cet-12518	53	13	lower	low	ADJ
cet-12518	53	14	,	,	PUNCT
cet-12518	53	15	which	which	PRON
cet-12518	53	16	may	may	AUX
cet-12518	53	17	imply	imply	VERB
cet-12518	53	18	deficiencies	deficiency	NOUN
cet-12518	53	19	in	in	ADP
cet-12518	53	20	the	the	DET
cet-12518	53	21	transfer	transfer	NOUN
cet-12518	53	22	of	of	ADP
cet-12518	53	23	oxygen	oxygen	NOUN
cet-12518	53	24	and	and	CCONJ
cet-12518	53	25	mass	mass	NOUN
cet-12518	53	26	within	within	ADP
cet-12518	53	27	the	the	DET
cet-12518	53	28	system	system	NOUN
cet-12518	53	29	.	.	PUNCT
cet-12518	54	1	higher	high	ADJ
cet-12518	54	2	efficient	efficient	ADJ
cet-12518	54	3	oxygen	oxygen	NOUN
cet-12518	54	4	transfer	transfer	NOUN
cet-12518	54	5	includes	include	VERB
cet-12518	54	6	an	an	DET
cet-12518	54	7	improvement	improvement	NOUN
cet-12518	54	8	in	in	ADP
cet-12518	54	9	the	the	DET
cet-12518	54	10	yields	yield	NOUN
cet-12518	54	11	of	of	ADP
cet-12518	54	12	biomass	biomass	NOUN
cet-12518	54	13	product	product	NOUN
cet-12518	54	14	,	,	PUNCT
cet-12518	54	15	especially	especially	ADV
cet-12518	54	16	when	when	SCONJ
cet-12518	54	17	talking	talk	VERB
cet-12518	54	18	about	about	ADP
cet-12518	54	19	the	the	DET
cet-12518	54	20	expression	expression	NOUN
cet-12518	54	21	of	of	ADP
cet-12518	54	22	metabolites	metabolite	NOUN
cet-12518	54	23	associated	associate	VERB
cet-12518	54	24	with	with	ADP
cet-12518	54	25	growth	growth	NOUN
cet-12518	54	26	(	(	PUNCT
cet-12518	54	27	falco	falco	PROPN
cet-12518	54	28	et	et	PROPN
cet-12518	54	29	al	al	PROPN
cet-12518	54	30	.	.	PROPN
cet-12518	54	31	,	,	PUNCT
cet-12518	54	32	2014	2014	NUM
cet-12518	54	33	)	)	PUNCT
cet-12518	54	34	.	.	PUNCT
cet-12518	55	1	table	table	NOUN
cet-12518	55	2	2	2	NUM
cet-12518	55	3	:	:	PUNCT
cet-12518	55	4	protein	protein	NOUN
cet-12518	55	5	production	production	NOUN
cet-12518	55	6	at	at	ADP
cet-12518	55	7	flask	flask	NOUN
cet-12518	55	8	and	and	CCONJ
cet-12518	55	9	bioreactor	bioreactor	NOUN
cet-12518	55	10	level	level	NOUN
cet-12518	55	11	1average	1average	NUM
cet-12518	55	12	production	production	NOUN
cet-12518	55	13	enzyme	enzyme	NOUN
cet-12518	55	14	;	;	PUNCT
cet-12518	55	15	2percentage	2percentage	NUM
cet-12518	55	16	improvement	improvement	NOUN
cet-12518	55	17	production	production	NOUN
cet-12518	55	18	compared	compare	VERB
cet-12518	55	19	whit	whit	NOUN
cet-12518	55	20	flask	flask	NOUN
cet-12518	55	21	level	level	NOUN
cet-12518	55	22	0,0	0,0	NUM
cet-12518	55	23	0,2	0,2	NUM
cet-12518	55	24	0,4	0,4	NUM
cet-12518	55	25	0,6	0,6	NUM
cet-12518	55	26	0,8	0,8	NUM
cet-12518	55	27	1,0	1,0	NUM
cet-12518	55	28	1,2	1,2	NUM
cet-12518	55	29	1,4	1,4	NUM
cet-12518	55	30	1,6	1,6	NUM
cet-12518	55	31	1	1	NUM
cet-12518	55	32	2	2	NUM
cet-12518	55	33	3	3	NUM
cet-12518	55	34	4	4	NUM
cet-12518	55	35	5	5	NUM
cet-12518	55	36	6	6	NUM
cet-12518	55	37	7	7	NUM
cet-12518	55	38	8	8	NUM
cet-12518	55	39	9	9	NUM
cet-12518	55	40	o	o	NOUN
cet-12518	55	41	pt	pt	NOUN
cet-12518	56	1	ic	ic	PROPN
cet-12518	56	2	al	al	PROPN
cet-12518	56	3	d	d	PROPN
cet-12518	56	4	en	en	ADP
cet-12518	56	5	si	si	PROPN
cet-12518	56	6	ty	ty	INTJ
cet-12518	56	7	5	5	NUM
cet-12518	56	8	95	95	NUM
cet-12518	56	9	(	(	PUNCT
cet-12518	56	10	n	n	NOUN
cet-12518	56	11	m	m	NOUN
cet-12518	56	12	)	)	PUNCT
cet-12518	56	13	time	time	NOUN
cet-12518	56	14	(	(	PUNCT
cet-12518	56	15	h	h	NOUN
cet-12518	56	16	)	)	PUNCT
cet-12518	56	17	biomass	biomass	NOUN
cet-12518	56	18	production	production	NOUN
cet-12518	56	19	at	at	ADP
cet-12518	56	20	350	350	NUM
cet-12518	56	21	rpm	rpm	ADJ
cet-12518	56	22	test	test	NOUN
cet-12518	56	23	1	1	NUM
cet-12518	56	24	test	test	NOUN
cet-12518	56	25	2	2	NUM
cet-12518	56	26	0,0	0,0	NUM
cet-12518	56	27	0,2	0,2	NUM
cet-12518	56	28	0,4	0,4	NUM
cet-12518	56	29	0,6	0,6	NUM
cet-12518	56	30	0,8	0,8	NUM
cet-12518	56	31	1,0	1,0	NUM
cet-12518	56	32	1,2	1,2	NUM
cet-12518	56	33	1,4	1,4	NUM
cet-12518	56	34	1	1	NUM
cet-12518	56	35	2	2	NUM
cet-12518	56	36	3	3	NUM
cet-12518	56	37	4	4	NUM
cet-12518	56	38	5	5	NUM
cet-12518	56	39	6	6	NUM
cet-12518	56	40	7	7	NUM
cet-12518	56	41	8	8	NUM
cet-12518	56	42	9	9	NUM
cet-12518	56	43	o	o	NOUN
cet-12518	56	44	pt	pt	NOUN
cet-12518	57	1	ic	ic	PROPN
cet-12518	57	2	al	al	PROPN
cet-12518	57	3	d	d	PROPN
cet-12518	57	4	en	en	ADP
cet-12518	57	5	si	si	PROPN
cet-12518	57	6	ty	ty	INTJ
cet-12518	57	7	5	5	NUM
cet-12518	57	8	95	95	NUM
cet-12518	57	9	(	(	PUNCT
cet-12518	57	10	n	n	NOUN
cet-12518	57	11	m	m	NOUN
cet-12518	57	12	)	)	PUNCT
cet-12518	57	13	time	time	NOUN
cet-12518	57	14	(	(	PUNCT
cet-12518	57	15	h	h	NOUN
cet-12518	57	16	)	)	PUNCT
cet-12518	57	17	biomass	biomass	NOUN
cet-12518	57	18	production	production	NOUN
cet-12518	57	19	at	at	ADP
cet-12518	57	20	220	220	NUM
cet-12518	57	21	rpm	rpm	ADJ
cet-12518	57	22	test	test	NOUN
cet-12518	57	23	1	1	NUM
cet-12518	57	24	test	test	NOUN
cet-12518	57	25	2	2	NUM
cet-12518	57	26	a	a	DET
cet-12518	57	27	)	)	PUNCT
cet-12518	57	28	b	b	NOUN
cet-12518	57	29	)	)	PUNCT
cet-12518	57	30	agitation	agitation	NOUN
cet-12518	57	31	(	(	PUNCT
cet-12518	57	32	rpm	rpm	NOUN
cet-12518	57	33	)	)	PUNCT
cet-12518	57	34	test	test	NOUN
cet-12518	57	35	nic1	nic1	NOUN
cet-12518	57	36	(	(	PUNCT
cet-12518	57	37	µg	µg	NOUN
cet-12518	57	38	/	/	SYM
cet-12518	57	39	ml	ml	NOUN
cet-12518	57	40	)	)	PUNCT
cet-12518	57	41	anic2	anic2	NOUN
cet-12518	57	42	(	(	PUNCT
cet-12518	57	43	µg	µg	NOUN
cet-12518	57	44	/	/	SYM
cet-12518	57	45	ml	ml	NOUN
cet-12518	57	46	)	)	PUNCT
cet-12518	57	47	ic3	ic3	PROPN
cet-12518	57	48	(	(	PUNCT
cet-12518	57	49	µg	µg	NOUN
cet-12518	57	50	/	/	SYM
cet-12518	57	51	ml	ml	NOUN
cet-12518	57	52	)	)	PUNCT
cet-12518	57	53	aic4	aic4	PROPN
cet-12518	57	54	(	(	PUNCT
cet-12518	57	55	µg	µg	NOUN
cet-12518	57	56	/	/	SYM
cet-12518	57	57	ml	ml	NOUN
cet-12518	57	58	)	)	PUNCT
cet-12518	57	59	220	220	NUM
cet-12518	57	60	1	1	NUM
cet-12518	57	61	857	857	NUM
cet-12518	57	62	883.25	883.25	NUM
cet-12518	57	63	1352.66	1352.66	NUM
cet-12518	57	64	1465.83	1465.83	NUM
cet-12518	57	65	2	2	NUM
cet-12518	57	66	909.5	909.5	NUM
cet-12518	57	67	1579	1579	NUM
cet-12518	57	68	350	350	NUM
cet-12518	57	69	1	1	NUM
cet-12518	57	70	1141	1141	NUM
cet-12518	57	71	1197.75	1197.75	NUM
cet-12518	57	72	3071	3071	NUM
cet-12518	57	73	2973.5	2973.5	NUM
cet-12518	57	74	2	2	NUM
cet-12518	57	75	1254.5	1254.5	NUM
cet-12518	57	76	2876	2876	NUM
cet-12518	57	77	scale	scale	NOUN
cet-12518	57	78	agitation	agitation	NOUN
cet-12518	57	79	(	(	PUNCT
cet-12518	57	80	rpm	rpm	PROPN
cet-12518	57	81	)	)	PUNCT
cet-12518	57	82	ape1	ape1	NOUN
cet-12518	57	83	(	(	PUNCT
cet-12518	57	84	µg	µg	NOUN
cet-12518	57	85	/	/	SYM
cet-12518	57	86	ml	ml	NOUN
cet-12518	57	87	)	)	PUNCT
cet-12518	57	88	pip2	pip2	NOUN
cet-12518	57	89	(	(	PUNCT
cet-12518	57	90	µg	µg	NOUN
cet-12518	57	91	/	/	SYM
cet-12518	57	92	ml	ml	NOUN
cet-12518	57	93	)	)	PUNCT
cet-12518	57	94	flask	flask	NOUN
cet-12518	57	95	210	210	NUM
cet-12518	57	96	(	(	PUNCT
cet-12518	57	97	orbital	orbital	ADJ
cet-12518	57	98	)	)	PUNCT
cet-12518	57	99	1633	1633	NUM
cet-12518	57	100	bioreactor	bioreactor	NOUN
cet-12518	57	101	350	350	NUM
cet-12518	57	102	2973.5	2973.5	NUM
cet-12518	57	103	82.08	82.08	NUM
cet-12518	57	104	220	220	NUM
cet-12518	57	105	1465.8	1465.8	NUM
cet-12518	57	106	-10.23	-10.23	SYM
cet-12518	57	107	123	123	NUM
cet-12518	57	108	it	it	PRON
cet-12518	57	109	is	be	AUX
cet-12518	57	110	important	important	ADJ
cet-12518	57	111	to	to	PART
cet-12518	57	112	clarify	clarify	VERB
cet-12518	57	113	that	that	SCONJ
cet-12518	57	114	the	the	DET
cet-12518	57	115	experimental	experimental	ADJ
cet-12518	57	116	processes	process	NOUN
cet-12518	57	117	at	at	ADP
cet-12518	57	118	flask	flask	NOUN
cet-12518	57	119	and	and	CCONJ
cet-12518	57	120	bioreactor	bioreactor	NOUN
cet-12518	57	121	scale	scale	NOUN
cet-12518	57	122	were	be	AUX
cet-12518	57	123	carried	carry	VERB
cet-12518	57	124	out	out	ADP
cet-12518	57	125	under	under	ADP
cet-12518	57	126	similar	similar	ADJ
cet-12518	57	127	conditions	condition	NOUN
cet-12518	57	128	such	such	ADJ
cet-12518	57	129	as	as	ADP
cet-12518	57	130	working	work	VERB
cet-12518	57	131	volume	volume	NOUN
cet-12518	57	132	(	(	PUNCT
cet-12518	57	133	400ml	400ml	PROPN
cet-12518	57	134	)	)	PUNCT
cet-12518	57	135	,	,	PUNCT
cet-12518	57	136	temperature	temperature	NOUN
cet-12518	57	137	,	,	PUNCT
cet-12518	57	138	culture	culture	NOUN
cet-12518	57	139	media	medium	NOUN
cet-12518	57	140	and	and	CCONJ
cet-12518	57	141	ph	ph	ADJ
cet-12518	57	142	,	,	PUNCT
cet-12518	57	143	so	so	ADV
cet-12518	57	144	a	a	DET
cet-12518	57	145	decrease	decrease	NOUN
cet-12518	57	146	in	in	ADP
cet-12518	57	147	the	the	DET
cet-12518	57	148	production	production	NOUN
cet-12518	57	149	of	of	ADP
cet-12518	57	150	taq	taq	NOUN
cet-12518	57	151	polymerase	polymerase	NOUN
cet-12518	57	152	at	at	ADP
cet-12518	57	153	the	the	DET
cet-12518	57	154	lowest	low	ADJ
cet-12518	57	155	speed	speed	NOUN
cet-12518	57	156	in	in	ADP
cet-12518	57	157	bioreactor	bioreactor	NOUN
cet-12518	57	158	suggests	suggest	VERB
cet-12518	57	159	an	an	DET
cet-12518	57	160	effect	effect	NOUN
cet-12518	57	161	on	on	ADP
cet-12518	57	162	enzyme	enzyme	NOUN
cet-12518	57	163	expression	expression	NOUN
cet-12518	57	164	;	;	PUNCT
cet-12518	57	165	however	however	ADV
cet-12518	57	166	,	,	PUNCT
cet-12518	57	167	this	this	PRON
cet-12518	57	168	requires	require	VERB
cet-12518	57	169	a	a	DET
cet-12518	57	170	greater	great	ADJ
cet-12518	57	171	number	number	NOUN
cet-12518	57	172	of	of	ADP
cet-12518	57	173	experiments	experiment	NOUN
cet-12518	57	174	to	to	PART
cet-12518	57	175	infer	infer	VERB
cet-12518	57	176	that	that	PRON
cet-12518	57	177	.	.	PUNCT
cet-12518	58	1	figure	figure	NOUN
cet-12518	58	2	2	2	NUM
cet-12518	58	3	.	.	NOUN
cet-12518	58	4	polyacrylamide	polyacrylamide	NOUN
cet-12518	58	5	gel	gel	NOUN
cet-12518	58	6	electrophoresis	electrophoresis	NOUN
cet-12518	58	7	for	for	ADP
cet-12518	58	8	the	the	DET
cet-12518	58	9	expression	expression	NOUN
cet-12518	58	10	and	and	CCONJ
cet-12518	58	11	purification	purification	NOUN
cet-12518	58	12	of	of	ADP
cet-12518	58	13	taq	taq	NOUN
cet-12518	58	14	polymerase	polymerase	NOUN
cet-12518	58	15	from	from	ADP
cet-12518	58	16	recombinant	recombinant	ADJ
cet-12518	58	17	e.	e.	PROPN
cet-12518	58	18	coli	coli	PROPN
cet-12518	58	19	tucan	tucan	PROPN
cet-12518	58	20	taq	taq	PROPN
cet-12518	58	21	®	®	PROPN
cet-12518	58	22	.	.	PUNCT
cet-12518	59	1	lane	lane	PROPN
cet-12518	59	2	m	m	PROPN
cet-12518	59	3	,	,	PUNCT
cet-12518	59	4	protein	protein	NOUN
cet-12518	59	5	molecular	molecular	ADJ
cet-12518	59	6	weight	weight	NOUN
cet-12518	59	7	marker	marker	NOUN
cet-12518	59	8	;	;	PUNCT
cet-12518	59	9	lane	lane	NOUN
cet-12518	59	10	1	1	NUM
cet-12518	59	11	,	,	PUNCT
cet-12518	59	12	e.	e.	PROPN
cet-12518	59	13	coli	coli	PROPN
cet-12518	59	14	after	after	ADP
cet-12518	59	15	inducing	induce	VERB
cet-12518	59	16	,	,	PUNCT
cet-12518	59	17	test	test	NOUN
cet-12518	59	18	1	1	NUM
cet-12518	59	19	at	at	ADP
cet-12518	59	20	350	350	NUM
cet-12518	59	21	rpm	rpm	NOUN
cet-12518	59	22	;	;	PUNCT
cet-12518	59	23	lane	lane	NOUN
cet-12518	59	24	2	2	NUM
cet-12518	59	25	,	,	PUNCT
cet-12518	59	26	e.	e.	PROPN
cet-12518	59	27	coli	coli	PROPN
cet-12518	59	28	before	before	ADP
cet-12518	59	29	induction	induction	NOUN
cet-12518	59	30	,	,	PUNCT
cet-12518	59	31	test	test	NOUN
cet-12518	59	32	1	1	NUM
cet-12518	59	33	at	at	ADP
cet-12518	59	34	350	350	NUM
cet-12518	59	35	rpm	rpm	NOUN
cet-12518	59	36	;	;	PUNCT
cet-12518	59	37	lane	lane	NOUN
cet-12518	59	38	3	3	NUM
cet-12518	59	39	,	,	PUNCT
cet-12518	59	40	e.	e.	PROPN
cet-12518	59	41	coli	coli	PROPN
cet-12518	59	42	after	after	ADP
cet-12518	59	43	inducing	induce	VERB
cet-12518	59	44	,	,	PUNCT
cet-12518	59	45	test	test	NOUN
cet-12518	59	46	2	2	NUM
cet-12518	59	47	at	at	ADP
cet-12518	59	48	350	350	NUM
cet-12518	59	49	rpm	rpm	NOUN
cet-12518	59	50	;	;	PUNCT
cet-12518	59	51	lane	lane	NOUN
cet-12518	59	52	4	4	NUM
cet-12518	59	53	,	,	PUNCT
cet-12518	59	54	e.	e.	PROPN
cet-12518	59	55	coli	coli	NOUN
cet-12518	59	56	before	before	ADP
cet-12518	59	57	inducing	induce	VERB
cet-12518	59	58	,	,	PUNCT
cet-12518	59	59	test	test	NOUN
cet-12518	59	60	2	2	NUM
cet-12518	59	61	at	at	ADP
cet-12518	59	62	350	350	NUM
cet-12518	59	63	rpm	rpm	NOUN
cet-12518	59	64	;	;	PUNCT
cet-12518	59	65	lane	lane	NOUN
cet-12518	59	66	5	5	NUM
cet-12518	59	67	,	,	PUNCT
cet-12518	59	68	e.	e.	PROPN
cet-12518	59	69	coli	coli	PROPN
cet-12518	59	70	after	after	ADP
cet-12518	59	71	inducing	induce	VERB
cet-12518	59	72	,	,	PUNCT
cet-12518	59	73	test	test	NOUN
cet-12518	59	74	2	2	NUM
cet-12518	59	75	at	at	ADP
cet-12518	59	76	220	220	NUM
cet-12518	59	77	rpm	rpm	NOUN
cet-12518	59	78	;	;	PUNCT
cet-12518	59	79	lane	lane	NOUN
cet-12518	59	80	6	6	NUM
cet-12518	59	81	,	,	PUNCT
cet-12518	59	82	e.	e.	PROPN
cet-12518	59	83	coli	coli	NOUN
cet-12518	59	84	before	before	ADP
cet-12518	59	85	inducing	induce	VERB
cet-12518	59	86	,	,	PUNCT
cet-12518	59	87	test	test	NOUN
cet-12518	59	88	2	2	NUM
cet-12518	59	89	at	at	ADP
cet-12518	59	90	220	220	NUM
cet-12518	59	91	rpm	rpm	NOUN
cet-12518	59	92	;	;	PUNCT
cet-12518	59	93	lane	lane	NOUN
cet-12518	59	94	7	7	NUM
cet-12518	59	95	,	,	PUNCT
cet-12518	59	96	e.	e.	PROPN
cet-12518	59	97	coli	coli	PROPN
cet-12518	59	98	after	after	ADP
cet-12518	59	99	inducing	induce	VERB
cet-12518	59	100	,	,	PUNCT
cet-12518	59	101	test	test	NOUN
cet-12518	59	102	1	1	NUM
cet-12518	59	103	at	at	ADP
cet-12518	59	104	220	220	NUM
cet-12518	59	105	rpm	rpm	NOUN
cet-12518	59	106	;	;	PUNCT
cet-12518	59	107	lane	lane	NOUN
cet-12518	59	108	8	8	NUM
cet-12518	59	109	,	,	PUNCT
cet-12518	59	110	e.	e.	PROPN
cet-12518	59	111	coli	coli	NOUN
cet-12518	59	112	before	before	ADP
cet-12518	59	113	inducing	induce	VERB
cet-12518	59	114	,	,	PUNCT
cet-12518	59	115	test	test	NOUN
cet-12518	59	116	1	1	NUM
cet-12518	59	117	at	at	ADP
cet-12518	59	118	220	220	NUM
cet-12518	59	119	rpm	rpm	NOUN
cet-12518	59	120	.	.	PUNCT
cet-12518	60	1	in	in	ADP
cet-12518	60	2	comparison	comparison	NOUN
cet-12518	60	3	with	with	ADP
cet-12518	60	4	the	the	DET
cet-12518	60	5	samples	sample	NOUN
cet-12518	60	6	before	before	ADP
cet-12518	60	7	induction	induction	NOUN
cet-12518	60	8	,	,	PUNCT
cet-12518	60	9	the	the	DET
cet-12518	60	10	absence	absence	NOUN
cet-12518	60	11	of	of	ADP
cet-12518	60	12	the	the	DET
cet-12518	60	13	expected	expect	VERB
cet-12518	60	14	band	band	NOUN
cet-12518	60	15	in	in	ADP
cet-12518	60	16	the	the	DET
cet-12518	60	17	molecular	molecular	ADJ
cet-12518	60	18	weight	weight	NOUN
cet-12518	60	19	of	of	ADP
cet-12518	60	20	taq	taq	NOUN
cet-12518	60	21	polymerase	polymerase	NOUN
cet-12518	60	22	is	be	AUX
cet-12518	60	23	evidenced	evidence	VERB
cet-12518	60	24	in	in	ADP
cet-12518	60	25	figure	figure	NOUN
cet-12518	60	26	2	2	NUM
cet-12518	60	27	,	,	PUNCT
cet-12518	60	28	indicating	indicate	VERB
cet-12518	60	29	that	that	SCONJ
cet-12518	60	30	these	these	DET
cet-12518	60	31	samples	sample	NOUN
cet-12518	60	32	do	do	AUX
cet-12518	60	33	not	not	PART
cet-12518	60	34	contain	contain	VERB
cet-12518	60	35	the	the	DET
cet-12518	60	36	protein	protein	NOUN
cet-12518	60	37	of	of	ADP
cet-12518	60	38	interest	interest	NOUN
cet-12518	60	39	.	.	PUNCT
cet-12518	61	1	for	for	ADP
cet-12518	61	2	all	all	DET
cet-12518	61	3	the	the	DET
cet-12518	61	4	replicates	replicate	NOUN
cet-12518	61	5	of	of	ADP
cet-12518	61	6	each	each	DET
cet-12518	61	7	level	level	NOUN
cet-12518	61	8	of	of	ADP
cet-12518	61	9	agitation	agitation	NOUN
cet-12518	61	10	,	,	PUNCT
cet-12518	61	11	it	it	PRON
cet-12518	61	12	is	be	AUX
cet-12518	61	13	observed	observe	VERB
cet-12518	61	14	that	that	SCONJ
cet-12518	61	15	the	the	DET
cet-12518	61	16	samples	sample	NOUN
cet-12518	61	17	after	after	ADP
cet-12518	61	18	induction	induction	NOUN
cet-12518	61	19	present	present	VERB
cet-12518	61	20	an	an	DET
cet-12518	61	21	intense	intense	ADJ
cet-12518	61	22	band	band	NOUN
cet-12518	61	23	at	at	ADP
cet-12518	61	24	a	a	DET
cet-12518	61	25	molecular	molecular	ADJ
cet-12518	61	26	weight	weight	NOUN
cet-12518	61	27	of	of	ADP
cet-12518	61	28	approximately	approximately	ADV
cet-12518	61	29	80	80	NUM
cet-12518	61	30	kda	kda	NOUN
cet-12518	61	31	,	,	PUNCT
cet-12518	61	32	indicating	indicate	VERB
cet-12518	61	33	the	the	DET
cet-12518	61	34	success	success	NOUN
cet-12518	61	35	of	of	ADP
cet-12518	61	36	the	the	DET
cet-12518	61	37	induction	induction	NOUN
cet-12518	61	38	and	and	CCONJ
cet-12518	61	39	expression	expression	NOUN
cet-12518	61	40	of	of	ADP
cet-12518	61	41	the	the	DET
cet-12518	61	42	enzyme	enzyme	NOUN
cet-12518	61	43	,	,	PUNCT
cet-12518	61	44	since	since	SCONJ
cet-12518	61	45	the	the	DET
cet-12518	61	46	presence	presence	NOUN
cet-12518	61	47	of	of	ADP
cet-12518	61	48	taq	taq	PROPN
cet-12518	61	49	is	be	AUX
cet-12518	61	50	evident	evident	ADJ
cet-12518	61	51	in	in	ADP
cet-12518	61	52	the	the	DET
cet-12518	61	53	different	different	ADJ
cet-12518	61	54	samples	sample	NOUN
cet-12518	61	55	.	.	PUNCT
cet-12518	62	1	although	although	SCONJ
cet-12518	62	2	the	the	DET
cet-12518	62	3	bradford	bradford	PROPN
cet-12518	62	4	technique	technique	NOUN
cet-12518	62	5	shows	show	VERB
cet-12518	62	6	an	an	DET
cet-12518	62	7	increase	increase	NOUN
cet-12518	62	8	in	in	ADP
cet-12518	62	9	protein	protein	NOUN
cet-12518	62	10	concentration	concentration	NOUN
cet-12518	62	11	at	at	ADP
cet-12518	62	12	350	350	NUM
cet-12518	62	13	rpm	rpm	NOUN
cet-12518	62	14	,	,	PUNCT
cet-12518	62	15	the	the	DET
cet-12518	62	16	gel	gel	NOUN
cet-12518	62	17	electrophoresis	electrophoresis	NOUN
cet-12518	62	18	technique	technique	NOUN
cet-12518	62	19	only	only	ADV
cet-12518	62	20	allows	allow	VERB
cet-12518	62	21	inferring	infer	VERB
cet-12518	62	22	whether	whether	SCONJ
cet-12518	62	23	the	the	DET
cet-12518	62	24	induction	induction	NOUN
cet-12518	62	25	was	be	AUX
cet-12518	62	26	successful	successful	ADJ
cet-12518	62	27	or	or	CCONJ
cet-12518	62	28	not	not	PART
cet-12518	62	29	.	.	PUNCT
cet-12518	63	1	3.3	3.3	NUM
cet-12518	63	2	measurement	measurement	NOUN
cet-12518	63	3	of	of	ADP
cet-12518	63	4	operation	operation	NOUN
cet-12518	63	5	parameters	parameter	NOUN
cet-12518	63	6	a	a	DET
cet-12518	63	7	change	change	NOUN
cet-12518	63	8	in	in	ADP
cet-12518	63	9	agitation	agitation	NOUN
cet-12518	63	10	speed	speed	NOUN
cet-12518	63	11	does	do	AUX
cet-12518	63	12	not	not	PART
cet-12518	63	13	generate	generate	VERB
cet-12518	63	14	a	a	DET
cet-12518	63	15	significant	significant	ADJ
cet-12518	63	16	difference	difference	NOUN
cet-12518	63	17	in	in	ADP
cet-12518	63	18	the	the	DET
cet-12518	63	19	measured	measured	ADJ
cet-12518	63	20	ph	ph	ADJ
cet-12518	63	21	values	value	NOUN
cet-12518	63	22	(	(	PUNCT
cet-12518	63	23	figure	figure	NOUN
cet-12518	63	24	3	3	NUM
cet-12518	63	25	)	)	PUNCT
cet-12518	63	26	;	;	PUNCT
cet-12518	63	27	in	in	ADP
cet-12518	63	28	addition	addition	NOUN
cet-12518	63	29	,	,	PUNCT
cet-12518	63	30	they	they	PRON
cet-12518	63	31	are	be	AUX
cet-12518	63	32	in	in	ADP
cet-12518	63	33	the	the	DET
cet-12518	63	34	optimal	optimal	ADJ
cet-12518	63	35	range	range	NOUN
cet-12518	63	36	for	for	ADP
cet-12518	63	37	growth	growth	NOUN
cet-12518	63	38	of	of	ADP
cet-12518	63	39	e.	e.	PROPN
cet-12518	63	40	coli	coli	PROPN
cet-12518	63	41	,	,	PUNCT
cet-12518	63	42	this	this	PRON
cet-12518	63	43	being	be	AUX
cet-12518	63	44	6	6	NUM
cet-12518	63	45	8.6	8.6	NUM
cet-12518	63	46	.	.	PUNCT
cet-12518	64	1	the	the	DET
cet-12518	64	2	ph	ph	PROPN
cet-12518	64	3	was	be	AUX
cet-12518	64	4	not	not	PART
cet-12518	64	5	controlled	control	VERB
cet-12518	64	6	by	by	ADP
cet-12518	64	7	adding	add	VERB
cet-12518	64	8	acids	acid	NOUN
cet-12518	64	9	and	and	CCONJ
cet-12518	64	10	bases	basis	NOUN
cet-12518	64	11	to	to	ADP
cet-12518	64	12	the	the	DET
cet-12518	64	13	fermentation	fermentation	NOUN
cet-12518	64	14	,	,	PUNCT
cet-12518	64	15	but	but	CCONJ
cet-12518	64	16	it	it	PRON
cet-12518	64	17	was	be	AUX
cet-12518	64	18	monitored	monitor	VERB
cet-12518	64	19	to	to	PART
cet-12518	64	20	ensure	ensure	VERB
cet-12518	64	21	that	that	SCONJ
cet-12518	64	22	the	the	DET
cet-12518	64	23	host	host	NOUN
cet-12518	64	24	and	and	CCONJ
cet-12518	64	25	the	the	DET
cet-12518	64	26	enzyme	enzyme	NOUN
cet-12518	64	27	of	of	ADP
cet-12518	64	28	interest	interest	NOUN
cet-12518	64	29	are	be	AUX
cet-12518	64	30	found	find	VERB
cet-12518	64	31	in	in	ADP
cet-12518	64	32	a	a	DET
cet-12518	64	33	growth	growth	NOUN
cet-12518	64	34	medium	medium	NOUN
cet-12518	64	35	that	that	PRON
cet-12518	64	36	will	will	AUX
cet-12518	64	37	generate	generate	VERB
cet-12518	64	38	a	a	DET
cet-12518	64	39	neutral	neutral	ADJ
cet-12518	64	40	ph	ph	ADJ
cet-12518	64	41	range	range	NOUN
cet-12518	64	42	.	.	PUNCT
cet-12518	65	1	according	accord	VERB
cet-12518	65	2	to	to	ADP
cet-12518	65	3	different	different	ADJ
cet-12518	65	4	studies	study	NOUN
cet-12518	65	5	of	of	ADP
cet-12518	65	6	taq	taq	NOUN
cet-12518	65	7	polymerase	polymerase	NOUN
cet-12518	65	8	production	production	NOUN
cet-12518	65	9	,	,	PUNCT
cet-12518	65	10	the	the	DET
cet-12518	65	11	reported	report	VERB
cet-12518	65	12	ph	ph	NOUN
cet-12518	65	13	is	be	AUX
cet-12518	65	14	the	the	DET
cet-12518	65	15	one	one	NUM
cet-12518	65	16	provided	provide	VERB
cet-12518	65	17	by	by	ADP
cet-12518	65	18	the	the	DET
cet-12518	65	19	culture	culture	NOUN
cet-12518	65	20	medium	medium	NOUN
cet-12518	65	21	used	use	VERB
cet-12518	65	22	,	,	PUNCT
cet-12518	65	23	being	be	AUX
cet-12518	65	24	the	the	DET
cet-12518	65	25	lb	lb	NOUN
cet-12518	65	26	,	,	PUNCT
cet-12518	65	27	with	with	ADP
cet-12518	65	28	a	a	DET
cet-12518	65	29	ph	ph	NOUN
cet-12518	65	30	of	of	ADP
cet-12518	65	31	6.8	6.8	NUM
cet-12518	65	32	to	to	PART
cet-12518	65	33	7.2	7.2	NUM
cet-12518	65	34	,	,	PUNCT
cet-12518	65	35	it	it	PRON
cet-12518	65	36	should	should	AUX
cet-12518	65	37	be	be	AUX
cet-12518	65	38	clarified	clarify	VERB
cet-12518	65	39	that	that	SCONJ
cet-12518	65	40	this	this	DET
cet-12518	65	41	production	production	NOUN
cet-12518	65	42	was	be	AUX
cet-12518	65	43	carried	carry	VERB
cet-12518	65	44	out	out	ADP
cet-12518	65	45	in	in	ADP
cet-12518	65	46	a	a	DET
cet-12518	65	47	flask	flask	NOUN
cet-12518	65	48	(	(	PUNCT
cet-12518	65	49	farazmandfar	farazmandfar	ADV
cet-12518	65	50	et	et	PROPN
cet-12518	65	51	al	al	PROPN
cet-12518	65	52	.	.	PROPN
cet-12518	65	53	,	,	PUNCT
cet-12518	65	54	2013	2013	NUM
cet-12518	65	55	)	)	PUNCT
cet-12518	65	56	.	.	PUNCT
cet-12518	66	1	the	the	DET
cet-12518	66	2	effect	effect	NOUN
cet-12518	66	3	of	of	ADP
cet-12518	66	4	agitation	agitation	NOUN
cet-12518	66	5	on	on	ADP
cet-12518	66	6	the	the	DET
cet-12518	66	7	availability	availability	NOUN
cet-12518	66	8	of	of	ADP
cet-12518	66	9	dissolved	dissolved	ADJ
cet-12518	66	10	oxygen	oxygen	NOUN
cet-12518	66	11	(	(	PUNCT
cet-12518	66	12	figure	figure	NOUN
cet-12518	66	13	4	4	NUM
cet-12518	66	14	)	)	PUNCT
cet-12518	66	15	in	in	ADP
cet-12518	66	16	the	the	DET
cet-12518	66	17	medium	medium	NOUN
cet-12518	66	18	is	be	AUX
cet-12518	66	19	significant	significant	ADJ
cet-12518	66	20	,	,	PUNCT
cet-12518	66	21	generating	generate	VERB
cet-12518	66	22	a	a	DET
cet-12518	66	23	decrease	decrease	NOUN
cet-12518	66	24	in	in	ADP
cet-12518	66	25	this	this	DET
cet-12518	66	26	element	element	NOUN
cet-12518	66	27	for	for	ADP
cet-12518	66	28	5	5	NUM
cet-12518	66	29	hours	hour	NOUN
cet-12518	66	30	when	when	SCONJ
cet-12518	66	31	the	the	DET
cet-12518	66	32	agitation	agitation	NOUN
cet-12518	66	33	was	be	AUX
cet-12518	66	34	at	at	ADP
cet-12518	66	35	220	220	NUM
cet-12518	66	36	rpm	rpm	NOUN
cet-12518	66	37	compared	compare	VERB
cet-12518	66	38	to	to	ADP
cet-12518	66	39	350	350	NUM
cet-12518	66	40	rpm	rpm	NOUN
cet-12518	66	41	where	where	SCONJ
cet-12518	66	42	the	the	DET
cet-12518	66	43	decrease	decrease	NOUN
cet-12518	66	44	was	be	AUX
cet-12518	66	45	for	for	ADP
cet-12518	66	46	1	1	NUM
cet-12518	66	47	hour	hour	NOUN
cet-12518	66	48	.	.	PUNCT
cet-12518	67	1	at	at	ADP
cet-12518	67	2	lower	low	ADJ
cet-12518	67	3	levels	level	NOUN
cet-12518	67	4	of	of	ADP
cet-12518	67	5	agitation	agitation	NOUN
cet-12518	67	6	,	,	PUNCT
cet-12518	67	7	cells	cell	NOUN
cet-12518	67	8	need	need	VERB
cet-12518	67	9	more	more	ADJ
cet-12518	67	10	time	time	NOUN
cet-12518	67	11	to	to	PART
cet-12518	67	12	be	be	AUX
cet-12518	67	13	able	able	ADJ
cet-12518	67	14	to	to	PART
cet-12518	67	15	adapt	adapt	VERB
cet-12518	67	16	their	their	PRON
cet-12518	67	17	metabolism	metabolism	NOUN
cet-12518	67	18	to	to	ADP
cet-12518	67	19	the	the	DET
cet-12518	67	20	metabolic	metabolic	NOUN
cet-12518	67	21	load	load	NOUN
cet-12518	67	22	imposed	impose	VERB
cet-12518	67	23	by	by	ADP
cet-12518	67	24	all	all	DET
cet-12518	67	25	the	the	DET
cet-12518	67	26	biological	biological	ADJ
cet-12518	67	27	processes	process	NOUN
cet-12518	67	28	carried	carry	VERB
cet-12518	67	29	out	out	ADP
cet-12518	67	30	(	(	PUNCT
cet-12518	67	31	sørensen	sørensen	NOUN
cet-12518	67	32	and	and	CCONJ
cet-12518	67	33	mortensen	mortensen	PROPN
cet-12518	67	34	,	,	PUNCT
cet-12518	67	35	2005	2005	NUM
cet-12518	67	36	)	)	PUNCT
cet-12518	67	37	.	.	PUNCT
cet-12518	68	1	at	at	ADP
cet-12518	68	2	350	350	NUM
cet-12518	68	3	rpm	rpm	NOUN
cet-12518	68	4	,	,	PUNCT
cet-12518	68	5	being	be	AUX
cet-12518	68	6	a	a	DET
cet-12518	68	7	higher	high	ADJ
cet-12518	68	8	agitation	agitation	NOUN
cet-12518	68	9	speed	speed	NOUN
cet-12518	68	10	,	,	PUNCT
cet-12518	68	11	a	a	DET
cet-12518	68	12	bubble	bubble	NOUN
cet-12518	68	13	size	size	NOUN
cet-12518	68	14	smaller	small	ADJ
cet-12518	68	15	than	than	ADP
cet-12518	68	16	220	220	NUM
cet-12518	68	17	rpm	rpm	NOUN
cet-12518	68	18	is	be	AUX
cet-12518	68	19	generated	generate	VERB
cet-12518	68	20	and	and	CCONJ
cet-12518	68	21	it	it	PRON
cet-12518	68	22	gives	give	VERB
cet-12518	68	23	a	a	DET
cet-12518	68	24	greater	great	ADJ
cet-12518	68	25	contact	contact	NOUN
cet-12518	68	26	area	area	NOUN
cet-12518	68	27	and	and	CCONJ
cet-12518	68	28	therefore	therefore	ADV
cet-12518	68	29	the	the	DET
cet-12518	68	30	diffusion	diffusion	NOUN
cet-12518	68	31	of	of	ADP
cet-12518	68	32	dissolved	dissolved	ADJ
cet-12518	68	33	oxygen	oxygen	NOUN
cet-12518	68	34	in	in	ADP
cet-12518	68	35	the	the	DET
cet-12518	68	36	medium	medium	NOUN
cet-12518	68	37	is	be	AUX
cet-12518	68	38	greater	great	ADJ
cet-12518	68	39	(	(	PUNCT
cet-12518	68	40	kulkarni	kulkarni	PROPN
cet-12518	68	41	et	et	PROPN
cet-12518	68	42	al	al	PROPN
cet-12518	68	43	.	.	PROPN
cet-12518	68	44	,	,	PUNCT
cet-12518	68	45	2009	2009	NUM
cet-12518	68	46	)	)	PUNCT
cet-12518	68	47	.	.	PUNCT
cet-12518	69	1	124	124	NUM
cet-12518	69	2	figure	figure	NOUN
cet-12518	69	3	3	3	NUM
cet-12518	69	4	:	:	PUNCT
cet-12518	69	5	ph	ph	ADJ
cet-12518	69	6	behavior	behavior	NOUN
cet-12518	69	7	at	at	ADP
cet-12518	69	8	different	different	ADJ
cet-12518	69	9	agitation	agitation	NOUN
cet-12518	69	10	speeds	speed	VERB
cet-12518	69	11	a	a	DET
cet-12518	69	12	)	)	PUNCT
cet-12518	69	13	220	220	NUM
cet-12518	69	14	rpm	rpm	NOUN
cet-12518	69	15	,	,	PUNCT
cet-12518	69	16	b	b	NOUN
cet-12518	69	17	)	)	PUNCT
cet-12518	69	18	350	350	NUM
cet-12518	69	19	rpm	rpm	NOUN
cet-12518	69	20	.	.	PUNCT
cet-12518	70	1	figure	figure	VERB
cet-12518	70	2	4	4	NUM
cet-12518	70	3	:	:	PUNCT
cet-12518	70	4	dissolved	dissolve	VERB
cet-12518	70	5	oxygen	oxygen	NOUN
cet-12518	70	6	behaviour	behaviour	NOUN
cet-12518	70	7	at	at	ADP
cet-12518	70	8	different	different	ADJ
cet-12518	70	9	agitation	agitation	NOUN
cet-12518	70	10	speeds	speed	VERB
cet-12518	70	11	a	a	PRON
cet-12518	70	12	)	)	PUNCT
cet-12518	70	13	220	220	NUM
cet-12518	70	14	rpm	rpm	NOUN
cet-12518	70	15	,	,	PUNCT
cet-12518	70	16	b	b	NOUN
cet-12518	70	17	)	)	PUNCT
cet-12518	70	18	350	350	NUM
cet-12518	70	19	rpm	rpm	NOUN
cet-12518	70	20	4	4	NUM
cet-12518	70	21	.	.	PUNCT
cet-12518	71	1	conclusions	conclusion	NOUN
cet-12518	71	2	continuous	continuous	ADJ
cet-12518	71	3	mechanical	mechanical	ADJ
cet-12518	71	4	agitation	agitation	NOUN
cet-12518	71	5	of	of	ADP
cet-12518	71	6	the	the	DET
cet-12518	71	7	bioreactor	bioreactor	NOUN
cet-12518	71	8	influences	influence	VERB
cet-12518	71	9	greatly	greatly	ADV
cet-12518	71	10	on	on	ADP
cet-12518	71	11	the	the	DET
cet-12518	71	12	concentration	concentration	NOUN
cet-12518	71	13	of	of	ADP
cet-12518	71	14	the	the	DET
cet-12518	71	15	recombinant	recombinant	ADJ
cet-12518	71	16	protein	protein	NOUN
cet-12518	71	17	of	of	ADP
cet-12518	71	18	taq	taq	NOUN
cet-12518	71	19	polymerase	polymerase	NOUN
cet-12518	71	20	expressed	express	VERB
cet-12518	71	21	in	in	ADP
cet-12518	71	22	escherichia	escherichia	NOUN
cet-12518	71	23	coli	coli	NOUN
cet-12518	71	24	.	.	PUNCT
cet-12518	72	1	it	it	PRON
cet-12518	72	2	increases	increase	VERB
cet-12518	72	3	the	the	DET
cet-12518	72	4	concentration	concentration	NOUN
cet-12518	72	5	at	at	ADP
cet-12518	72	6	higher	high	ADJ
cet-12518	72	7	levels	level	NOUN
cet-12518	72	8	of	of	ADP
cet-12518	72	9	agitation	agitation	NOUN
cet-12518	72	10	,	,	PUNCT
cet-12518	72	11	on	on	ADP
cet-12518	72	12	average	average	ADJ
cet-12518	72	13	82	82	NUM
cet-12518	72	14	%	%	NOUN
cet-12518	72	15	in	in	ADP
cet-12518	72	16	the	the	DET
cet-12518	72	17	level	level	NOUN
cet-12518	72	18	of	of	ADP
cet-12518	72	19	350	350	NUM
cet-12518	72	20	rpm	rpm	NOUN
cet-12518	72	21	compared	compare	VERB
cet-12518	72	22	to	to	ADP
cet-12518	72	23	flask	flask	NOUN
cet-12518	72	24	scale	scale	NOUN
cet-12518	72	25	.	.	PUNCT
cet-12518	73	1	production	production	NOUN
cet-12518	73	2	of	of	ADP
cet-12518	73	3	taq	taq	NOUN
cet-12518	73	4	polymerase	polymerase	NOUN
cet-12518	73	5	at	at	ADP
cet-12518	73	6	a	a	DET
cet-12518	73	7	bioreactor	bioreactor	NOUN
cet-12518	73	8	scale	scale	NOUN
cet-12518	73	9	is	be	AUX
cet-12518	73	10	of	of	ADP
cet-12518	73	11	great	great	ADJ
cet-12518	73	12	importance	importance	NOUN
cet-12518	73	13	since	since	SCONJ
cet-12518	73	14	the	the	DET
cet-12518	73	15	demand	demand	NOUN
cet-12518	73	16	for	for	ADP
cet-12518	73	17	the	the	DET
cet-12518	73	18	pcr	pcr	ADJ
cet-12518	73	19	technique	technique	NOUN
cet-12518	73	20	in	in	ADP
cet-12518	73	21	the	the	DET
cet-12518	73	22	world	world	NOUN
cet-12518	73	23	has	have	AUX
cet-12518	73	24	increased	increase	VERB
cet-12518	73	25	considerably	considerably	ADV
cet-12518	73	26	.	.	PUNCT
cet-12518	74	1	finally	finally	ADV
cet-12518	74	2	,	,	PUNCT
cet-12518	74	3	bioreactors	bioreactor	NOUN
cet-12518	74	4	for	for	ADP
cet-12518	74	5	production	production	NOUN
cet-12518	74	6	of	of	ADP
cet-12518	74	7	recombinant	recombinant	ADJ
cet-12518	74	8	proteins	protein	NOUN
cet-12518	74	9	have	have	VERB
cet-12518	74	10	easier	easy	ADJ
cet-12518	74	11	monitoring	monitoring	NOUN
cet-12518	74	12	and	and	CCONJ
cet-12518	74	13	control	control	NOUN
cet-12518	74	14	of	of	ADP
cet-12518	74	15	the	the	DET
cet-12518	74	16	different	different	ADJ
cet-12518	74	17	environmental	environmental	ADJ
cet-12518	74	18	parameters	parameter	NOUN
cet-12518	74	19	providing	provide	VERB
cet-12518	74	20	advantages	advantage	NOUN
cet-12518	74	21	when	when	SCONJ
cet-12518	74	22	it	it	PRON
cet-12518	74	23	comes	come	VERB
cet-12518	74	24	to	to	ADP
cet-12518	74	25	an	an	DET
cet-12518	74	26	industrial	industrial	ADJ
cet-12518	74	27	process	process	NOUN
cet-12518	74	28	.	.	PUNCT
cet-12518	75	1	acknowledgments	acknowledgment	NOUN
cet-12518	75	2	the	the	DET
cet-12518	75	3	authors	author	NOUN
cet-12518	75	4	express	express	VERB
cet-12518	75	5	sincere	sincere	ADJ
cet-12518	75	6	gratitude	gratitude	NOUN
cet-12518	75	7	,	,	PUNCT
cet-12518	75	8	especially	especially	ADV
cet-12518	75	9	to	to	ADP
cet-12518	75	10	corporation	corporation	NOUN
cet-12518	75	11	corpogen	corpogen	X
cet-12518	75	12	(	(	PUNCT
cet-12518	75	13	private	private	ADJ
cet-12518	75	14	non	non	ADJ
cet-12518	75	15	-	-	ADJ
cet-12518	75	16	profit	profit	ADJ
cet-12518	75	17	research	research	NOUN
cet-12518	75	18	center	center	NOUN
cet-12518	75	19	,	,	PUNCT
cet-12518	75	20	https://en.corpogen.org/	https://en.corpogen.org/	PROPN
cet-12518	75	21	)	)	PUNCT
cet-12518	75	22	,	,	PUNCT
cet-12518	75	23	the	the	DET
cet-12518	75	24	researcher	researcher	NOUN
cet-12518	75	25	dayana	dayana	PROPN
cet-12518	75	26	calderon	calderon	NOUN
cet-12518	75	27	and	and	CCONJ
cet-12518	75	28	fundación	fundación	PROPN
cet-12518	75	29	universidad	universidad	PROPN
cet-12518	75	30	de	de	PROPN
cet-12518	75	31	américa	américa	PROPN
cet-12518	75	32	,	,	PUNCT
cet-12518	75	33	for	for	ADP
cet-12518	75	34	supporting	support	VERB
cet-12518	75	35	this	this	DET
cet-12518	75	36	research	research	NOUN
cet-12518	75	37	references	reference	NOUN
cet-12518	75	38	bagagli	bagagli	NOUN
cet-12518	75	39	m.p	m.p	PROPN
cet-12518	75	40	and	and	CCONJ
cet-12518	75	41	sato	sato	PROPN
cet-12518	75	42	h.	h.	PROPN
cet-12518	75	43	,	,	PUNCT
cet-12518	75	44	2013	2013	NUM
cet-12518	75	45	,	,	PUNCT
cet-12518	75	46	two	two	NUM
cet-12518	75	47	-	-	PUNCT
cet-12518	75	48	staged	stage	VERB
cet-12518	75	49	temperature	temperature	NOUN
cet-12518	75	50	and	and	CCONJ
cet-12518	75	51	agitation	agitation	NOUN
cet-12518	75	52	strategy	strategy	NOUN
cet-12518	75	53	for	for	ADP
cet-12518	75	54	the	the	DET
cet-12518	75	55	production	production	NOUN
cet-12518	75	56	of	of	ADP
cet-12518	75	57	transglutaminase	transglutaminase	NOUN
cet-12518	75	58	from	from	ADP
cet-12518	75	59	a	a	DET
cet-12518	75	60	streptomyces	streptomyce	NOUN
cet-12518	75	61	sp	sp	NOUN
cet-12518	75	62	.	.	NOUN
cet-12518	75	63	isolated	isolate	VERB
cet-12518	75	64	from	from	ADP
cet-12518	75	65	brazilian	brazilian	ADJ
cet-12518	75	66	soil	soil	NOUN
cet-12518	75	67	.	.	PUNCT
cet-12518	76	1	applied	apply	VERB
cet-12518	76	2	biochem	biochem	PROPN
cet-12518	76	3	biotechnol	biotechnol	NOUN
cet-12518	76	4	,	,	PUNCT
cet-12518	76	5	170	170	NUM
cet-12518	76	6	,	,	PUNCT
cet-12518	76	7	1057	1057	NUM
cet-12518	76	8	-	-	SYM
cet-12518	76	9	1065	1065	NUM
cet-12518	76	10	bradford	bradford	PROPN
cet-12518	76	11	mm	mm	PROPN
cet-12518	76	12	.	.	PROPN
cet-12518	76	13	,	,	PUNCT
cet-12518	76	14	1976	1976	NUM
cet-12518	76	15	,	,	PUNCT
cet-12518	76	16	a	a	DET
cet-12518	76	17	rapid	rapid	ADJ
cet-12518	76	18	and	and	CCONJ
cet-12518	76	19	sensitive	sensitive	ADJ
cet-12518	76	20	method	method	NOUN
cet-12518	76	21	for	for	ADP
cet-12518	76	22	the	the	DET
cet-12518	76	23	quantification	quantification	NOUN
cet-12518	76	24	of	of	ADP
cet-12518	76	25	microgram	microgram	ADJ
cet-12518	76	26	quantities	quantity	NOUN
cet-12518	76	27	of	of	ADP
cet-12518	76	28	protein	protein	NOUN
cet-12518	76	29	-	-	PUNCT
cet-12518	76	30	dye	dye	NOUN
cet-12518	76	31	binding	bind	VERB
cet-12518	76	32	,	,	PUNCT
cet-12518	76	33	analytical	analytical	ADJ
cet-12518	76	34	biochemestry	biochemestry	NOUN
cet-12518	76	35	,	,	PUNCT
cet-12518	76	36	72	72	NUM
cet-12518	76	37	,	,	PUNCT
cet-12518	76	38	248	248	NUM
cet-12518	76	39	-	-	SYM
cet-12518	76	40	254	254	NUM
cet-12518	76	41	biorad	biorad	NOUN
cet-12518	76	42	2020	2020	NUM
cet-12518	76	43	.	.	PUNCT
cet-12518	77	1	introduction	introduction	NOUN
cet-12518	77	2	to	to	ADP
cet-12518	77	3	protein	protein	NOUN
cet-12518	77	4	electrophoresis	electrophoresis	NOUN
cet-12518	77	5	.	.	PUNCT
cet-12518	78	1	available	available	ADJ
cet-12518	78	2	at	at	ADP
cet-12518	78	3	www.bio-rad.com/en-us/applicationstechnologies/introduction-protein-electrophoresis?id=lusovo47b	www.bio-rad.com/en-us/applicationstechnologies/introduction-protein-electrophoresis?id=lusovo47b	PROPN
cet-12518	78	4	che	che	PROPN
cet-12518	78	5	m.r	m.r	PROPN
cet-12518	78	6	.	.	PROPN
cet-12518	78	7	,	,	PUNCT
cet-12518	78	8	fauzi	fauzi	PROPN
cet-12518	78	9	i.a	i.a	PROPN
cet-12518	78	10	.	.	PROPN
cet-12518	78	11	,	,	PUNCT
cet-12518	78	12	siti	siti	PROPN
cet-12518	78	13	f.z	f.z	PROPN
cet-12518	78	14	.	.	PROPN
cet-12518	78	15	,	,	PUNCT
cet-12518	78	16	ghazali	ghazali	PROPN
cet-12518	78	17	n.	n.	PROPN
cet-12518	78	18	,	,	PUNCT
cet-12518	78	19	md	md	PROPN
cet-12518	78	20	i.r	i.r	PROPN
cet-12518	78	21	.	.	PROPN
cet-12518	78	22	,2016	,2016	PUNCT
cet-12518	78	23	,	,	PUNCT
cet-12518	78	24	effects	effect	NOUN
cet-12518	78	25	of	of	ADP
cet-12518	78	26	culture	culture	NOUN
cet-12518	78	27	conditions	condition	NOUN
cet-12518	78	28	of	of	ADP
cet-12518	78	29	immobilized	immobilized	ADJ
cet-12518	78	30	recombinant	recombinant	ADJ
cet-12518	78	31	escherichia	escherichia	NOUN
cet-12518	78	32	coli	coli	NOUN
cet-12518	78	33	on	on	ADP
cet-12518	78	34	cyclodextrin	cyclodextrin	NOUN
cet-12518	78	35	glucanotransferase	glucanotransferase	NOUN
cet-12518	78	36	(	(	PUNCT
cet-12518	78	37	cgtase	cgtase	NOUN
cet-12518	78	38	)	)	PUNCT
cet-12518	78	39	excretion	excretion	NOUN
cet-12518	78	40	and	and	CCONJ
cet-12518	78	41	cell	cell	NOUN
cet-12518	78	42	stability	stability	NOUN
cet-12518	78	43	,	,	PUNCT
cet-12518	78	44	process	process	NOUN
cet-12518	78	45	biochemistry	biochemistry	NOUN
cet-12518	78	46	,	,	PUNCT
cet-12518	78	47	51	51	NUM
cet-12518	78	48	(	(	PUNCT
cet-12518	78	49	4	4	NUM
cet-12518	78	50	)	)	PUNCT
cet-12518	78	51	,	,	PUNCT
cet-12518	78	52	474	474	NUM
cet-12518	78	53	-	-	SYM
cet-12518	78	54	483	483	NUM
cet-12518	78	55	5,0	5,0	NUM
cet-12518	78	56	5,5	5,5	NUM
cet-12518	79	1	6,0	6,0	NUM
cet-12518	79	2	6,5	6,5	NUM
cet-12518	79	3	7,0	7,0	NUM
cet-12518	79	4	7,5	7,5	NUM
cet-12518	79	5	8,0	8,0	NUM
cet-12518	79	6	8,5	8,5	NUM
cet-12518	79	7	9,0	9,0	NOUN
cet-12518	79	8	1	1	NUM
cet-12518	79	9	2	2	NUM
cet-12518	79	10	3	3	NUM
cet-12518	79	11	4	4	NUM
cet-12518	79	12	5	5	NUM
cet-12518	79	13	6	6	NUM
cet-12518	79	14	7	7	NUM
cet-12518	79	15	8	8	NUM
cet-12518	79	16	9	9	NUM
cet-12518	79	17	10	10	NUM
cet-12518	79	18	11	11	NUM
cet-12518	79	19	12	12	NUM
cet-12518	79	20	13	13	NUM
cet-12518	79	21	14	14	NUM
cet-12518	79	22	15	15	NUM
cet-12518	79	23	16	16	NUM
cet-12518	79	24	17	17	NUM
cet-12518	79	25	18	18	NUM
cet-12518	79	26	19	19	NUM
cet-12518	79	27	20	20	NUM
cet-12518	79	28	21	21	NUM
cet-12518	79	29	ph	ph	NOUN
cet-12518	79	30	time	time	NOUN
cet-12518	79	31	(	(	PUNCT
cet-12518	79	32	h	h	NOUN
cet-12518	79	33	)	)	PUNCT
cet-12518	79	34	ph	ph	NOUN
cet-12518	79	35	at	at	ADP
cet-12518	79	36	350	350	NUM
cet-12518	79	37	rpm	rpm	ADJ
cet-12518	79	38	test	test	NOUN
cet-12518	79	39	1	1	NUM
cet-12518	79	40	test	test	NOUN
cet-12518	79	41	2	2	NUM
cet-12518	79	42	5,0	5,0	NUM
cet-12518	79	43	5,5	5,5	NUM
cet-12518	79	44	6,0	6,0	NUM
cet-12518	79	45	6,5	6,5	NUM
cet-12518	79	46	7,0	7,0	NUM
cet-12518	79	47	7,5	7,5	NUM
cet-12518	79	48	8,0	8,0	NUM
cet-12518	79	49	8,5	8,5	NUM
cet-12518	79	50	9,0	9,0	NOUN
cet-12518	79	51	1	1	NUM
cet-12518	79	52	2	2	NUM
cet-12518	79	53	3	3	NUM
cet-12518	79	54	4	4	NUM
cet-12518	79	55	5	5	NUM
cet-12518	79	56	6	6	NUM
cet-12518	79	57	7	7	NUM
cet-12518	79	58	8	8	NUM
cet-12518	79	59	9	9	NUM
cet-12518	79	60	10	10	NUM
cet-12518	79	61	11	11	NUM
cet-12518	79	62	12	12	NUM
cet-12518	79	63	13	13	NUM
cet-12518	79	64	14	14	NUM
cet-12518	79	65	15	15	NUM
cet-12518	79	66	16	16	NUM
cet-12518	79	67	17	17	NUM
cet-12518	79	68	18	18	NUM
cet-12518	79	69	19	19	NUM
cet-12518	79	70	20	20	NUM
cet-12518	79	71	21	21	NUM
cet-12518	79	72	ph	ph	NOUN
cet-12518	79	73	time	time	NOUN
cet-12518	79	74	(	(	PUNCT
cet-12518	79	75	h	h	NOUN
cet-12518	79	76	)	)	PUNCT
cet-12518	79	77	ph	ph	NOUN
cet-12518	79	78	at	at	ADP
cet-12518	79	79	220	220	NUM
cet-12518	79	80	rpm	rpm	ADJ
cet-12518	79	81	test	test	NOUN
cet-12518	79	82	1	1	NUM
cet-12518	79	83	test	test	NOUN
cet-12518	79	84	2	2	NUM
cet-12518	79	85	a	a	PRON
cet-12518	79	86	)	)	PUNCT
cet-12518	79	87	b	b	NOUN
cet-12518	79	88	)	)	PUNCT
cet-12518	79	89	0	0	NUM
cet-12518	79	90	20	20	NUM
cet-12518	79	91	40	40	NUM
cet-12518	79	92	60	60	NUM
cet-12518	79	93	80	80	NUM
cet-12518	79	94	100	100	NUM
cet-12518	79	95	1	1	NUM
cet-12518	79	96	2	2	NUM
cet-12518	79	97	3	3	NUM
cet-12518	79	98	4	4	NUM
cet-12518	79	99	5	5	NUM
cet-12518	79	100	6	6	NUM
cet-12518	79	101	7	7	NUM
cet-12518	79	102	8	8	NUM
cet-12518	79	103	9	9	NUM
cet-12518	79	104	10	10	NUM
cet-12518	79	105	11	11	NUM
cet-12518	79	106	12	12	NUM
cet-12518	79	107	13	13	NUM
cet-12518	79	108	14	14	NUM
cet-12518	79	109	15	15	NUM
cet-12518	79	110	16	16	NUM
cet-12518	79	111	17	17	NUM
cet-12518	79	112	18	18	NUM
cet-12518	79	113	19	19	NUM
cet-12518	79	114	20	20	NUM
cet-12518	79	115	21	21	NUM
cet-12518	79	116	22	22	NUM
cet-12518	79	117	d	d	NOUN
cet-12518	79	118	is	be	AUX
cet-12518	79	119	so	so	ADV
cet-12518	79	120	lv	lv	PROPN
cet-12518	80	1	ed	ed	NOUN
cet-12518	81	1	o	o	X
cet-12518	81	2	xy	xy	INTJ
cet-12518	81	3	ge	ge	PROPN
cet-12518	81	4	n	n	PROPN
cet-12518	81	5	(	(	PUNCT
cet-12518	81	6	%	%	NOUN
cet-12518	81	7	)	)	PUNCT
cet-12518	81	8	time	time	NOUN
cet-12518	81	9	(	(	PUNCT
cet-12518	81	10	h	h	NOUN
cet-12518	81	11	)	)	PUNCT
cet-12518	81	12	dissolved	dissolve	VERB
cet-12518	81	13	oxygen	oxygen	NOUN
cet-12518	81	14	at	at	ADP
cet-12518	81	15	220	220	NUM
cet-12518	81	16	rpm	rpm	ADJ
cet-12518	81	17	test	test	NOUN
cet-12518	81	18	1	1	NUM
cet-12518	81	19	test	test	NOUN
cet-12518	81	20	2	2	NUM
cet-12518	81	21	0	0	NUM
cet-12518	81	22	20	20	NUM
cet-12518	81	23	40	40	NUM
cet-12518	81	24	60	60	NUM
cet-12518	81	25	80	80	NUM
cet-12518	81	26	100	100	NUM
cet-12518	81	27	1	1	NUM
cet-12518	81	28	2	2	NUM
cet-12518	81	29	3	3	NUM
cet-12518	81	30	4	4	NUM
cet-12518	81	31	5	5	NUM
cet-12518	81	32	6	6	NUM
cet-12518	81	33	7	7	NUM
cet-12518	81	34	8	8	NUM
cet-12518	81	35	9	9	NUM
cet-12518	81	36	10	10	NUM
cet-12518	81	37	11	11	NUM
cet-12518	81	38	12	12	NUM
cet-12518	81	39	13	13	NUM
cet-12518	81	40	14	14	NUM
cet-12518	81	41	15	15	NUM
cet-12518	81	42	16	16	NUM
cet-12518	81	43	17	17	NUM
cet-12518	81	44	18	18	NUM
cet-12518	81	45	19	19	NUM
cet-12518	81	46	20	20	NUM
cet-12518	81	47	21	21	NUM
cet-12518	81	48	d	d	NOUN
cet-12518	81	49	is	be	AUX
cet-12518	81	50	so	so	ADV
cet-12518	81	51	lv	lv	PROPN
cet-12518	82	1	ed	ed	NOUN
cet-12518	83	1	o	o	X
cet-12518	83	2	xy	xy	INTJ
cet-12518	83	3	ge	ge	PROPN
cet-12518	83	4	n	n	PROPN
cet-12518	83	5	(	(	PUNCT
cet-12518	83	6	%	%	NOUN
cet-12518	83	7	)	)	PUNCT
cet-12518	83	8	time	time	NOUN
cet-12518	83	9	(	(	PUNCT
cet-12518	83	10	h	h	NOUN
cet-12518	83	11	)	)	PUNCT
cet-12518	83	12	dissolved	dissolve	VERB
cet-12518	83	13	oxygen	oxygen	NOUN
cet-12518	83	14	at	at	ADP
cet-12518	83	15	350	350	NUM
cet-12518	83	16	rpm	rpm	ADJ
cet-12518	83	17	test	test	NOUN
cet-12518	83	18	1	1	NUM
cet-12518	83	19	test	test	NOUN
cet-12518	83	20	2	2	NUM
cet-12518	83	21	a	a	PRON
cet-12518	83	22	)	)	PUNCT
cet-12518	83	23	b	b	NOUN
cet-12518	83	24	)	)	PUNCT
cet-12518	83	25	125	125	NUM
cet-12518	83	26	https://en.corpogen.org/	https://en.corpogen.org/	PROPN
cet-12518	83	27	eppendorf	eppendorf	NOUN
cet-12518	83	28	.	.	PUNCT
cet-12518	83	29	,	,	PUNCT
cet-12518	83	30	2009	2009	NUM
cet-12518	83	31	.	.	PUNCT
cet-12518	84	1	bioflo	bioflo	PROPN
cet-12518	84	2	®	®	PROPN
cet-12518	84	3	/celligen	/celligen	SYM
cet-12518	84	4	®	®	NOUN
cet-12518	84	5	115	115	NUM
cet-12518	84	6	.	.	PUNCT
cet-12518	85	1	advantage	advantage	NOUN
cet-12518	85	2	business	business	NOUN
cet-12518	85	3	media	medium	NOUN
cet-12518	85	4	.	.	PUNCT
cet-12518	86	1	espinosa	espinosa	PROPN
cet-12518	86	2	,	,	PUNCT
cet-12518	86	3	r.	r.	PROPN
cet-12518	86	4	,	,	PUNCT
cet-12518	86	5	garcía	garcía	PROPN
cet-12518	86	6	,	,	PUNCT
cet-12518	86	7	j.	j.	PROPN
cet-12518	86	8	,	,	PUNCT
cet-12518	86	9	narciandi	narciandi	PROPN
cet-12518	86	10	,	,	PUNCT
cet-12518	86	11	e.	e.	PROPN
cet-12518	86	12	,	,	PUNCT
cet-12518	86	13	silva	silva	PROPN
cet-12518	86	14	r.	r.	PROPN
cet-12518	86	15	,	,	PUNCT
cet-12518	86	16	caballero	caballero	PROPN
cet-12518	86	17	,	,	PUNCT
cet-12518	86	18	e.	e.	PROPN
cet-12518	86	19	,	,	PUNCT
cet-12518	86	20	díaz	díaz	PROPN
cet-12518	86	21	h.	h.	PROPN
cet-12518	86	22	,	,	PUNCT
cet-12518	86	23	musacchio	musacchio	VERB
cet-12518	86	24	a	a	PRON
cet-12518	86	25	,	,	PUNCT
cet-12518	86	26	2018	2018	NUM
cet-12518	86	27	.	.	PUNCT
cet-12518	87	1	scaling	scale	VERB
cet-12518	87	2	-	-	PUNCT
cet-12518	87	3	up	up	ADP
cet-12518	87	4	fermentation	fermentation	NOUN
cet-12518	87	5	of	of	ADP
cet-12518	87	6	escherichia	escherichia	NOUN
cet-12518	87	7	coli	coli	NOUN
cet-12518	87	8	for	for	ADP
cet-12518	87	9	production	production	NOUN
cet-12518	87	10	of	of	ADP
cet-12518	87	11	recombinant	recombinant	ADJ
cet-12518	87	12	p64k	p64k	X
cet-12518	87	13	protein	protein	NOUN
cet-12518	87	14	from	from	ADP
cet-12518	87	15	neisseria	neisseria	PROPN
cet-12518	87	16	meningitidis	meningitidi	NOUN
cet-12518	87	17	.	.	PUNCT
cet-12518	88	1	electronic	electronic	ADJ
cet-12518	88	2	journal	journal	NOUN
cet-12518	88	3	of	of	ADP
cet-12518	88	4	biotechnology	biotechnology	NOUN
cet-12518	88	5	,	,	PUNCT
cet-12518	88	6	33	33	NUM
cet-12518	88	7	,	,	PUNCT
cet-12518	88	8	29	29	NUM
cet-12518	88	9	-	-	SYM
cet-12518	88	10	35	35	NUM
cet-12518	88	11	.	.	PUNCT
cet-12518	89	1	falco	falco	PROPN
cet-12518	89	2	f.c	f.c	PROPN
cet-12518	89	3	.	.	PROPN
cet-12518	89	4	,	,	PUNCT
cet-12518	89	5	landi	landi	PROPN
cet-12518	89	6	c.	c.	PROPN
cet-12518	89	7	,	,	PUNCT
cet-12518	89	8	paciello	paciello	PROPN
cet-12518	89	9	l.	l.	PROPN
cet-12518	89	10	,	,	PUNCT
cet-12518	89	11	zueco	zueco	PROPN
cet-12518	89	12	j.	j.	PROPN
cet-12518	89	13	,	,	PUNCT
cet-12518	89	14	parascandola	parascandola	PROPN
cet-12518	89	15	p.	p.	PROPN
cet-12518	89	16	,	,	PUNCT
cet-12518	89	17	2014	2014	NUM
cet-12518	89	18	,	,	PUNCT
cet-12518	89	19	fed	feed	VERB
cet-12518	89	20	-	-	PUNCT
cet-12518	89	21	batch	batch	NOUN
cet-12518	89	22	production	production	NOUN
cet-12518	89	23	of	of	ADP
cet-12518	89	24	endoglucanase	endoglucanase	NOUN
cet-12518	89	25	with	with	ADP
cet-12518	89	26	a	a	DET
cet-12518	89	27	recombinant	recombinant	ADJ
cet-12518	89	28	industrial	industrial	ADJ
cet-12518	89	29	stran	stran	NOUN
cet-12518	89	30	of	of	ADP
cet-12518	89	31	the	the	DET
cet-12518	89	32	yeast	yeast	NOUN
cet-12518	89	33	saccharomyces	saccharomyce	NOUN
cet-12518	89	34	cerevisiae	cerevisiae	NOUN
cet-12518	89	35	,	,	PUNCT
cet-12518	89	36	chemical	chemical	ADJ
cet-12518	89	37	engineering	engineering	NOUN
cet-12518	89	38	transactions	transaction	NOUN
cet-12518	89	39	,	,	PUNCT
cet-12518	89	40	38	38	NUM
cet-12518	89	41	,	,	PUNCT
cet-12518	89	42	379	379	NUM
cet-12518	89	43	-	-	SYM
cet-12518	89	44	384	384	NUM
cet-12518	89	45	.	.	PUNCT
cet-12518	90	1	farazmandfar	farazmandfar	PROPN
cet-12518	90	2	,	,	PUNCT
cet-12518	90	3	t.	t.	PROPN
cet-12518	90	4	,	,	PUNCT
cet-12518	90	5	rafiei	rafiei	PROPN
cet-12518	90	6	,	,	PUNCT
cet-12518	90	7	a.	a.	PROPN
cet-12518	90	8	,	,	PUNCT
cet-12518	90	9	hashemi	hashemi	NOUN
cet-12518	90	10	-	-	PUNCT
cet-12518	90	11	sotehoh	sotehoh	PROPN
cet-12518	90	12	,	,	PUNCT
cet-12518	90	13	m.	m.	PROPN
cet-12518	90	14	b.	b.	PROPN
cet-12518	90	15	,	,	PUNCT
cet-12518	90	16	valadan	valadan	PROPN
cet-12518	90	17	,	,	PUNCT
cet-12518	90	18	r.	r.	PROPN
cet-12518	90	19	,	,	PUNCT
cet-12518	90	20	alavi	alavi	NOUN
cet-12518	90	21	,	,	PUNCT
cet-12518	90	22	m.	m.	NOUN
cet-12518	90	23	,	,	PUNCT
cet-12518	90	24	moradian	moradian	ADJ
cet-12518	90	25	,	,	PUNCT
cet-12518	90	26	f	f	X
cet-12518	90	27	,	,	PUNCT
cet-12518	90	28	2013	2013	NUM
cet-12518	90	29	,	,	PUNCT
cet-12518	90	30	a	a	DET
cet-12518	90	31	simplified	simplified	ADJ
cet-12518	90	32	protocol	protocol	NOUN
cet-12518	90	33	for	for	ADP
cet-12518	90	34	producing	produce	VERB
cet-12518	90	35	taq	taq	NOUN
cet-12518	90	36	dna	dna	PROPN
cet-12518	90	37	polymerase	polymerase	NOUN
cet-12518	90	38	in	in	ADP
cet-12518	90	39	biology	biology	NOUN
cet-12518	90	40	laboratory	laboratory	NOUN
cet-12518	90	41	.	.	PUNCT
cet-12518	91	1	research	research	NOUN
cet-12518	91	2	in	in	ADP
cet-12518	91	3	molecular	molecular	ADJ
cet-12518	91	4	medicine	medicine	NOUN
cet-12518	91	5	,	,	PUNCT
cet-12518	91	6	1(2	1(2	NUM
cet-12518	91	7	)	)	PUNCT
cet-12518	91	8	,	,	PUNCT
cet-12518	91	9	23	23	NUM
cet-12518	91	10	-	-	SYM
cet-12518	91	11	26	26	NUM
cet-12518	91	12	.	.	PUNCT
cet-12518	92	1	ferralli	ferralli	PROPN
cet-12518	92	2	,	,	PUNCT
cet-12518	92	3	p.	p.	PROPN
cet-12518	92	4	,	,	PUNCT
cet-12518	92	5	egan	egan	PROPN
cet-12518	92	6	j.	j.	PROPN
cet-12518	92	7	d.	d.	PROPN
cet-12518	92	8	,	,	PUNCT
cet-12518	92	9	erickson	erickson	PROPN
cet-12518	92	10	,	,	PUNCT
cet-12518	92	11	f.	f.	PROPN
cet-12518	92	12	l.	l.	PROPN
cet-12518	92	13	,	,	PUNCT
cet-12518	92	14	2007	2007	NUM
cet-12518	92	15	,	,	PUNCT
cet-12518	92	16	making	make	VERB
cet-12518	92	17	taq	taq	PROPN
cet-12518	92	18	dna	dna	PROPN
cet-12518	92	19	polymerase	polymerase	NOUN
cet-12518	92	20	in	in	ADP
cet-12518	92	21	the	the	DET
cet-12518	92	22	undergraduate	undergraduate	NOUN
cet-12518	92	23	biology	biology	NOUN
cet-12518	92	24	laboratory	laboratory	NOUN
cet-12518	92	25	.	.	PUNCT
cet-12518	93	1	bios	bios	PROPN
cet-12518	93	2	journal	journal	NOUN
cet-12518	93	3	,	,	PUNCT
cet-12518	93	4	69	69	NUM
cet-12518	93	5	-	-	SYM
cet-12518	93	6	74	74	NUM
cet-12518	93	7	.	.	PUNCT
cet-12518	94	1	kulkarni	kulkarni	PROPN
cet-12518	94	2	a.	a.	PROPN
cet-12518	94	3	v.	v.	PROPN
cet-12518	94	4	,	,	PUNCT
cet-12518	94	5	badgandi	badgandi	VERB
cet-12518	94	6	s.	s.	PROPN
cet-12518	94	7	v.	v.	PROPN
cet-12518	94	8	,	,	PUNCT
cet-12518	94	9	joshi	joshi	PROPN
cet-12518	94	10	j.	j.	PROPN
cet-12518	94	11	b.	b.	PROPN
cet-12518	94	12	,	,	PUNCT
cet-12518	94	13	2009	2009	NUM
cet-12518	94	14	,	,	PUNCT
cet-12518	94	15	design	design	NOUN
cet-12518	94	16	of	of	ADP
cet-12518	94	17	ring	ring	NOUN
cet-12518	94	18	and	and	CCONJ
cet-12518	94	19	spider	spider	NOUN
cet-12518	94	20	type	type	NOUN
cet-12518	94	21	spargers	sparger	NOUN
cet-12518	94	22	for	for	ADP
cet-12518	94	23	bubble	bubble	NOUN
cet-12518	94	24	column	column	NOUN
cet-12518	94	25	reactor	reactor	NOUN
cet-12518	94	26	:	:	PUNCT
cet-12518	94	27	experimental	experimental	ADJ
cet-12518	94	28	measurements	measurement	NOUN
cet-12518	94	29	and	and	CCONJ
cet-12518	94	30	cfd	cfd	NOUN
cet-12518	94	31	simulation	simulation	NOUN
cet-12518	94	32	of	of	ADP
cet-12518	94	33	flow	flow	NOUN
cet-12518	94	34	and	and	CCONJ
cet-12518	94	35	weeping	weeping	NOUN
cet-12518	94	36	.	.	PUNCT
cet-12518	95	1	chemical	chemical	NOUN
cet-12518	95	2	engineering	engineering	PROPN
cet-12518	95	3	research	research	NOUN
cet-12518	95	4	and	and	CCONJ
cet-12518	95	5	design	design	NOUN
cet-12518	95	6	,	,	PUNCT
cet-12518	95	7	87(12	87(12	NUM
cet-12518	95	8	)	)	PUNCT
cet-12518	95	9	,	,	PUNCT
cet-12518	95	10	1612	1612	NUM
cet-12518	95	11	-	-	SYM
cet-12518	95	12	1630	1630	NUM
cet-12518	95	13	.	.	PUNCT
cet-12518	96	1	liu	liu	PROPN
cet-12518	96	2	s.	s.	PROPN
cet-12518	96	3	,	,	PUNCT
cet-12518	96	4	2020	2020	NUM
cet-12518	96	5	,	,	PUNCT
cet-12518	96	6	bioreactor	bioreactor	NOUN
cet-12518	96	7	design	design	NOUN
cet-12518	96	8	operation	operation	NOUN
cet-12518	96	9	,	,	PUNCT
cet-12518	96	10	chapter	chapter	NOUN
cet-12518	96	11	17	17	NUM
cet-12518	96	12	in	in	ADP
cet-12518	96	13	bioprocess	bioprocess	NOUN
cet-12518	96	14	engineering	engineering	NOUN
cet-12518	96	15	,	,	PUNCT
cet-12518	96	16	third	third	ADJ
cet-12518	96	17	edition	edition	NOUN
cet-12518	96	18	.	.	PUNCT
cet-12518	97	1	isbn	isbn	ADJ
cet-12518	97	2	978	978	NUM
cet-12518	97	3	-	-	SYM
cet-12518	97	4	00444	00444	NUM
cet-12518	97	5	-	-	PUNCT
cet-12518	97	6	63783	63783	NUM
cet-12518	97	7	-	-	PUNCT
cet-12518	97	8	3	3	NUM
cet-12518	97	9	.	.	X
cet-12518	97	10	merck	merck	PROPN
cet-12518	97	11	.	.	PROPN
cet-12518	97	12	,	,	PUNCT
cet-12518	97	13	2020	2020	NUM
cet-12518	97	14	caldo	caldo	NOUN
cet-12518	97	15	lb	lb	DET
cet-12518	97	16	miller	miller	NOUN
cet-12518	97	17	www.merckmillipore.com/co/es/product/lb-broth-miller,mda_chem-110285	www.merckmillipore.com/co/es/product/lb-broth-miller,mda_chem-110285	PUNCT
cet-12518	97	18	singh	singh	PROPN
cet-12518	97	19	a.k	a.k	PROPN
cet-12518	97	20	.	.	PROPN
cet-12518	97	21	,	,	PUNCT
cet-12518	97	22	shrivastava	shrivastava	PROPN
cet-12518	97	23	s.	s.	PROPN
cet-12518	97	24	,	,	PUNCT
cet-12518	97	25	kumar	kumar	PROPN
cet-12518	97	26	s.	s.	PROPN
cet-12518	97	27	,	,	PUNCT
cet-12518	97	28	pal	pal	NOUN
cet-12518	97	29	v.	v.	ADV
cet-12518	97	30	,	,	PUNCT
cet-12518	97	31	gopalan	gopalan	PROPN
cet-12518	97	32	n.	n.	PROPN
cet-12518	97	33	,2015	,2015	PUNCT
cet-12518	97	34	,	,	PUNCT
cet-12518	97	35	batch	batch	VERB
cet-12518	97	36	fermentation	fermentation	NOUN
cet-12518	97	37	of	of	ADP
cet-12518	97	38	recombinant	recombinant	ADJ
cet-12518	97	39	burkholderia	burkholderia	NOUN
cet-12518	97	40	intracellular	intracellular	ADJ
cet-12518	97	41	motility	motility	NOUN
cet-12518	97	42	a	a	DET
cet-12518	97	43	protein	protein	NOUN
cet-12518	97	44	in	in	ADP
cet-12518	97	45	escherichia	escherichia	NOUN
cet-12518	97	46	coli	coli	NOUN
cet-12518	97	47	for	for	ADP
cet-12518	97	48	the	the	DET
cet-12518	97	49	diagnosis	diagnosis	NOUN
cet-12518	97	50	of	of	ADP
cet-12518	97	51	equine	equine	NOUN
cet-12518	97	52	glander	glander	NOUN
cet-12518	97	53	.	.	PUNCT
cet-12518	98	1	journal	journal	PROPN
cet-12518	98	2	of	of	ADP
cet-12518	98	3	equine	equine	NOUN
cet-12518	98	4	veterinary	veterinary	ADJ
cet-12518	98	5	science	science	NOUN
cet-12518	98	6	,	,	PUNCT
cet-12518	98	7	35	35	NUM
cet-12518	98	8	(	(	PUNCT
cet-12518	98	9	2	2	NUM
cet-12518	98	10	)	)	PUNCT
cet-12518	98	11	,	,	PUNCT
cet-12518	98	12	124	124	NUM
cet-12518	98	13	-	-	SYM
cet-12518	98	14	129	129	NUM
cet-12518	98	15	.	.	PUNCT
cet-12518	99	1	sørensen	sørensen	PROPN
cet-12518	99	2	h.	h.	PROPN
cet-12518	99	3	p.	p.	PROPN
cet-12518	99	4	,	,	PUNCT
cet-12518	99	5	mortensen	mortensen	PROPN
cet-12518	99	6	k.	k.	PROPN
cet-12518	99	7	k.	k.	PROPN
cet-12518	99	8	,	,	PUNCT
cet-12518	99	9	2005	2005	NUM
cet-12518	99	10	,	,	PUNCT
cet-12518	99	11	soluble	soluble	ADJ
cet-12518	99	12	expression	expression	NOUN
cet-12518	99	13	of	of	ADP
cet-12518	99	14	recombinant	recombinant	ADJ
cet-12518	99	15	proteins	protein	NOUN
cet-12518	99	16	in	in	ADP
cet-12518	99	17	the	the	DET
cet-12518	99	18	cytoplasm	cytoplasm	NOUN
cet-12518	99	19	of	of	ADP
cet-12518	99	20	escherichia	escherichia	NOUN
cet-12518	99	21	coli	coli	NOUN
cet-12518	99	22	.	.	PUNCT
cet-12518	100	1	microbial	microbial	ADJ
cet-12518	100	2	cell	cell	NOUN
cet-12518	100	3	factories	factory	NOUN
cet-12518	100	4	,	,	PUNCT
cet-12518	100	5	4(1	4(1	NOUN
cet-12518	100	6	)	)	PUNCT
cet-12518	100	7	,	,	PUNCT
cet-12518	100	8	1	1	NUM
cet-12518	100	9	-	-	SYM
cet-12518	100	10	8	8	NUM
cet-12518	100	11	.	.	PUNCT
cet-12518	100	12	sung	sung	NOUN
cet-12518	100	13	-	-	PUNCT
cet-12518	100	14	gun	gun	NOUN
cet-12518	100	15	k	k	PROPN
cet-12518	100	16	and	and	CCONJ
cet-12518	100	17	jong	jong	PROPN
cet-12518	100	18	-	-	PUNCT
cet-12518	100	19	tae	tae	PROPN
cet-12518	100	20	p.	p.	NOUN
cet-12518	100	21	,2014	,2014	PUNCT
cet-12518	100	22	,	,	PUNCT
cet-12518	100	23	production	production	NOUN
cet-12518	100	24	of	of	ADP
cet-12518	100	25	dna	dna	PROPN
cet-12518	100	26	polymerase	polymerase	NOUN
cet-12518	100	27	from	from	ADP
cet-12518	100	28	thermus	thermus	PROPN
cet-12518	100	29	aquaticus	aquaticus	VERB
cet-12518	100	30	in	in	ADP
cet-12518	100	31	recombinant	recombinant	ADJ
cet-12518	100	32	escherichia	escherichia	NOUN
cet-12518	100	33	coli	coli	NOUN
cet-12518	100	34	.	.	PUNCT
cet-12518	101	1	korean	korean	ADJ
cet-12518	101	2	journal	journal	PROPN
cet-12518	101	3	of	of	ADP
cet-12518	101	4	agricultural	agricultural	ADJ
cet-12518	101	5	science	science	NOUN
cet-12518	101	6	,	,	PUNCT
cet-12518	101	7	41(3	41(3	NUM
cet-12518	101	8	)	)	PUNCT
cet-12518	101	9	,	,	PUNCT
cet-12518	101	10	245	245	NUM
cet-12518	101	11	-	-	SYM
cet-12518	101	12	249	249	NUM
cet-12518	101	13	.	.	PUNCT
cet-12518	102	1	tan	tan	PROPN
cet-12518	102	2	j.s	j.s	PROPN
cet-12518	102	3	.	.	PROPN
cet-12518	102	4	,	,	PUNCT
cet-12518	102	5	abbasiliasi	abbasiliasi	PROPN
cet-12518	102	6	s.	s.	PROPN
cet-12518	102	7	,	,	PUNCT
cet-12518	102	8	tam	tam	PROPN
cet-12518	102	9	j.y	j.y	PROPN
cet-12518	102	10	.	.	PROPN
cet-12518	102	11	,	,	PUNCT
cet-12518	102	12	tang	tang	PROPN
cet-12518	103	1	t.k	t.k	PROPN
cet-12518	103	2	,	,	PUNCT
cet-12518	103	3	lee	lee	PROPN
cet-12518	103	4	y	y	PROPN
cet-12518	103	5	-	-	PUNCT
cet-12518	103	6	y.	y.	PROPN
cet-12518	103	7	,	,	PUNCT
cet-12518	103	8	ariff	ariff	PROPN
cet-12518	103	9	a.b	a.b	PROPN
cet-12518	103	10	,	,	PUNCT
cet-12518	103	11	2018	2018	NUM
cet-12518	103	12	,	,	PUNCT
cet-12518	103	13	microtiter	microtiter	NOUN
cet-12518	103	14	miniature	miniature	NOUN
cet-12518	103	15	shaken	shake	VERB
cet-12518	103	16	bioreactor	bioreactor	NOUN
cet-12518	103	17	system	system	NOUN
cet-12518	103	18	as	as	ADP
cet-12518	103	19	a	a	DET
cet-12518	103	20	scale	scale	NOUN
cet-12518	103	21	-	-	PUNCT
cet-12518	103	22	down	down	ADP
cet-12518	103	23	model	model	NOUN
cet-12518	103	24	for	for	ADP
cet-12518	103	25	process	process	NOUN
cet-12518	103	26	development	development	NOUN
cet-12518	103	27	of	of	ADP
cet-12518	103	28	production	production	NOUN
cet-12518	103	29	of	of	ADP
cet-12518	103	30	therapeutic	therapeutic	ADJ
cet-12518	103	31	alpha	alpha	NOUN
cet-12518	103	32	-	-	PUNCT
cet-12518	103	33	interferon2b	interferon2b	NOUN
cet-12518	103	34	by	by	ADP
cet-12518	103	35	recombinant	recombinant	ADJ
cet-12518	103	36	escherichia	escherichia	NOUN
cet-12518	103	37	coli	coli	NOUN
cet-12518	103	38	,	,	PUNCT
cet-12518	103	39	bmc	bmc	NOUN
cet-12518	103	40	microbiology	microbiology	NOUN
cet-12518	103	41	18:3	18:3	NUM
cet-12518	103	42	126	126	NUM
cet-12518	103	43	https://www.merckmillipore.com/co/es/product/lb-broth-miller,mda_chem-110285	https://www.merckmillipore.com/co/es/product/lb-broth-miller,mda_chem-110285	NOUN
cet-12518	103	44	33moralesfonseca.pdf	33moralesfonseca.pdf	NUM
cet-12518	103	45	effect	effect	NOUN
cet-12518	103	46	of	of	ADP
cet-12518	103	47	agitation	agitation	NOUN
cet-12518	103	48	on	on	ADP
cet-12518	103	49	taq	taq	PROPN
cet-12518	103	50	dna	dna	PROPN
cet-12518	103	51	polymerase	polymerase	NOUN
cet-12518	103	52	production	production	NOUN
cet-12518	103	53	by	by	ADP
cet-12518	103	54	escherichia	escherichia	NOUN
cet-12518	103	55	coli	coli	NOUN
cet-12518	103	56	in	in	ADP
cet-12518	103	57	bioreactor	bioreactor	NOUN