id	sid	tid	token	lemma	pos
cet-13508	1	1	doi	doi	NOUN
cet-13508	1	2	:	:	PUNCT
cet-13508	1	3	10.3303	10.3303	NUM
cet-13508	1	4	/	/	SYM
cet-13508	1	5	cet23102018	cet23102018	PROPN
cet-13508	1	6	paper	paper	NOUN
cet-13508	1	7	received	receive	VERB
cet-13508	1	8	:	:	PUNCT
cet-13508	1	9	23	23	NUM
cet-13508	1	10	february	february	NOUN
cet-13508	1	11	2023	2023	NUM
cet-13508	1	12	;	;	PUNCT
cet-13508	1	13	revised	revise	VERB
cet-13508	1	14	:	:	PUNCT
cet-13508	1	15	17	17	NUM
cet-13508	1	16	april	april	PROPN
cet-13508	1	17	2023	2023	NUM
cet-13508	1	18	;	;	PUNCT
cet-13508	1	19	accepted	accept	VERB
cet-13508	1	20	:	:	PUNCT
cet-13508	1	21	29	29	NUM
cet-13508	1	22	august	august	PROPN
cet-13508	1	23	2023	2023	NUM
cet-13508	1	24	please	please	INTJ
cet-13508	1	25	cite	cite	VERB
cet-13508	1	26	this	this	DET
cet-13508	1	27	article	article	NOUN
cet-13508	1	28	as	as	ADP
cet-13508	1	29	:	:	PUNCT
cet-13508	1	30	klemanska	klemanska	ADJ
cet-13508	1	31	a.	a.	NOUN
cet-13508	1	32	,	,	PUNCT
cet-13508	1	33	pisano	pisano	PROPN
cet-13508	1	34	i.	i.	PROPN
cet-13508	1	35	,	,	PUNCT
cet-13508	1	36	dwyer	dwyer	PROPN
cet-13508	1	37	k.	k.	PROPN
cet-13508	1	38	,	,	PUNCT
cet-13508	1	39	walsh	walsh	PROPN
cet-13508	1	40	g.	g.	PROPN
cet-13508	1	41	,	,	PUNCT
cet-13508	1	42	2023	2023	NUM
cet-13508	1	43	,	,	PUNCT
cet-13508	1	44	purification	purification	NOUN
cet-13508	1	45	and	and	CCONJ
cet-13508	1	46	characterisation	characterisation	NOUN
cet-13508	1	47	of	of	ADP
cet-13508	1	48	a	a	DET
cet-13508	1	49	fungal	fungal	ADJ
cet-13508	1	50	β-1,6	β-1,6	NOUN
cet-13508	1	51	-	-	NOUN
cet-13508	1	52	glucanase	glucanase	NOUN
cet-13508	1	53	for	for	ADP
cet-13508	1	54	the	the	DET
cet-13508	1	55	production	production	NOUN
cet-13508	1	56	of	of	ADP
cet-13508	1	57	gentio	gentio	NOUN
cet-13508	1	58	-	-	PUNCT
cet-13508	1	59	oligosaccharides	oligosaccharide	NOUN
cet-13508	1	60	from	from	ADP
cet-13508	1	61	brewer	brewer	NOUN
cet-13508	1	62	’s	’s	PART
cet-13508	1	63	spent	spend	VERB
cet-13508	1	64	yeast	yeast	NOUN
cet-13508	1	65	,	,	PUNCT
cet-13508	1	66	chemical	chemical	NOUN
cet-13508	1	67	engineering	engineering	NOUN
cet-13508	1	68	transactions	transaction	NOUN
cet-13508	1	69	,	,	PUNCT
cet-13508	1	70	102	102	NUM
cet-13508	1	71	,	,	PUNCT
cet-13508	1	72	103	103	NUM
cet-13508	1	73	-	-	SYM
cet-13508	1	74	108	108	NUM
cet-13508	1	75	doi:10.3303	doi:10.3303	NOUN
cet-13508	1	76	/	/	SYM
cet-13508	1	77	cet23102018	cet23102018	PROPN
cet-13508	1	78	chemical	chemical	PROPN
cet-13508	1	79	engineering	engineering	NOUN
cet-13508	1	80	transactions	transaction	NOUN
cet-13508	1	81	vol	vol	NOUN
cet-13508	1	82	.	.	PUNCT
cet-13508	2	1	102	102	NUM
cet-13508	2	2	,	,	PUNCT
cet-13508	2	3	2023	2023	NUM
cet-13508	2	4	a	a	DET
cet-13508	2	5	publication	publication	NOUN
cet-13508	2	6	of	of	ADP
cet-13508	2	7	the	the	DET
cet-13508	2	8	italian	italian	ADJ
cet-13508	2	9	association	association	NOUN
cet-13508	2	10	of	of	ADP
cet-13508	2	11	chemical	chemical	PROPN
cet-13508	2	12	engineering	engineering	NOUN
cet-13508	2	13	online	online	ADV
cet-13508	2	14	at	at	ADP
cet-13508	2	15	www.cetjournal.it	www.cetjournal.it	PROPN
cet-13508	2	16	guest	guest	NOUN
cet-13508	2	17	editors	editor	NOUN
cet-13508	2	18	:	:	PUNCT
cet-13508	2	19	laura	laura	PROPN
cet-13508	2	20	piazza	piazza	PROPN
cet-13508	2	21	,	,	PUNCT
cet-13508	2	22	mauro	mauro	PROPN
cet-13508	2	23	moresi	moresi	PROPN
cet-13508	2	24	,	,	PUNCT
cet-13508	2	25	francesco	francesco	PROPN
cet-13508	2	26	donsì	donsì	PROPN
cet-13508	2	27	copyright	copyright	NOUN
cet-13508	3	1	©	©	PROPN
cet-13508	3	2	2023	2023	NUM
cet-13508	3	3	,	,	PUNCT
cet-13508	3	4	aidic	aidic	ADJ
cet-13508	3	5	servizi	servizi	PROPN
cet-13508	3	6	s.r.l	s.r.l	NOUN
cet-13508	3	7	.	.	PUNCT
cet-13508	4	1	isbn	isbn	PROPN
cet-13508	4	2	979	979	NUM
cet-13508	4	3	-	-	SYM
cet-13508	4	4	12	12	NUM
cet-13508	4	5	-	-	PUNCT
cet-13508	4	6	81206	81206	NUM
cet-13508	4	7	-	-	PUNCT
cet-13508	4	8	01	01	NUM
cet-13508	4	9	-	-	PUNCT
cet-13508	4	10	4	4	NUM
cet-13508	4	11	;	;	PUNCT
cet-13508	4	12	issn	issn	PROPN
cet-13508	4	13	2283	2283	NUM
cet-13508	4	14	-	-	SYM
cet-13508	4	15	9216	9216	NUM
cet-13508	4	16	purification	purification	NOUN
cet-13508	4	17	and	and	CCONJ
cet-13508	4	18	characterisation	characterisation	NOUN
cet-13508	4	19	of	of	ADP
cet-13508	4	20	a	a	DET
cet-13508	4	21	fungal	fungal	ADJ
cet-13508	4	22	β-1,6	β-1,6	NOUN
cet-13508	4	23	-	-	NOUN
cet-13508	4	24	glucanase	glucanase	NOUN
cet-13508	4	25	for	for	ADP
cet-13508	4	26	the	the	DET
cet-13508	4	27	production	production	NOUN
cet-13508	4	28	of	of	ADP
cet-13508	4	29	gentio	gentio	NOUN
cet-13508	4	30	-	-	PUNCT
cet-13508	4	31	oligosaccharides	oligosaccharide	NOUN
cet-13508	4	32	from	from	ADP
cet-13508	4	33	brewer	brewer	NOUN
cet-13508	4	34	’s	’s	PART
cet-13508	4	35	spent	spend	VERB
cet-13508	4	36	yeast	yeast	NOUN
cet-13508	4	37	anastasia	anastasia	PROPN
cet-13508	4	38	klemanskaa	klemanskaa	PROPN
cet-13508	4	39	,	,	PUNCT
cet-13508	4	40	b	b	PROPN
cet-13508	4	41	*	*	PROPN
cet-13508	4	42	,	,	PUNCT
cet-13508	4	43	italo	italo	PROPN
cet-13508	4	44	pisanoa	pisanoa	PROPN
cet-13508	4	45	,	,	PUNCT
cet-13508	4	46	kelly	kelly	PROPN
cet-13508	4	47	dwyerb	dwyerb	PROPN
cet-13508	4	48	,	,	PUNCT
cet-13508	4	49	gary	gary	PROPN
cet-13508	4	50	walsha	walsha	PROPN
cet-13508	4	51	abernal	abernal	PROPN
cet-13508	4	52	institute	institute	PROPN
cet-13508	4	53	,	,	PUNCT
cet-13508	4	54	university	university	PROPN
cet-13508	4	55	of	of	ADP
cet-13508	4	56	limerick	limerick	PROPN
cet-13508	4	57	,	,	PUNCT
cet-13508	4	58	castletroy	castletroy	PROPN
cet-13508	4	59	,	,	PUNCT
cet-13508	4	60	limerick	limerick	PROPN
cet-13508	4	61	city	city	PROPN
cet-13508	4	62	,	,	PUNCT
cet-13508	4	63	ireland	ireland	NOUN
cet-13508	4	64	bmonaghan	bmonaghan	NOUN
cet-13508	4	65	mushrooms	mushroom	NOUN
cet-13508	4	66	,	,	PUNCT
cet-13508	4	67	tyholland	tyholland	PROPN
cet-13508	4	68	,	,	PUNCT
cet-13508	4	69	co.	co.	PROPN
cet-13508	4	70	monaghan	monaghan	PROPN
cet-13508	4	71	,	,	PUNCT
cet-13508	4	72	ireland	ireland	PROPN
cet-13508	4	73	anastasia.klemanska@ul.ie	anastasia.klemanska@ul.ie	NOUN
cet-13508	4	74	gentio	gentio	NOUN
cet-13508	4	75	-	-	PUNCT
cet-13508	4	76	oligosaccharides	oligosaccharide	NOUN
cet-13508	4	77	(	(	PUNCT
cet-13508	4	78	gnos	gnos	PROPN
cet-13508	4	79	)	)	PUNCT
cet-13508	4	80	are	be	AUX
cet-13508	4	81	an	an	DET
cet-13508	4	82	emerging	emerge	VERB
cet-13508	4	83	class	class	NOUN
cet-13508	4	84	of	of	ADP
cet-13508	4	85	prebiotics	prebiotic	NOUN
cet-13508	4	86	derived	derive	VERB
cet-13508	4	87	from	from	ADP
cet-13508	4	88	β-1,6	β-1,6	PROPN
cet-13508	4	89	-	-	PUNCT
cet-13508	4	90	glucan	glucan	NOUN
cet-13508	4	91	,	,	PUNCT
cet-13508	4	92	a	a	DET
cet-13508	4	93	polysaccharide	polysaccharide	NOUN
cet-13508	4	94	found	find	VERB
cet-13508	4	95	within	within	ADP
cet-13508	4	96	the	the	DET
cet-13508	4	97	cell	cell	NOUN
cet-13508	4	98	walls	wall	NOUN
cet-13508	4	99	of	of	ADP
cet-13508	4	100	fungi	fungus	NOUN
cet-13508	4	101	such	such	ADJ
cet-13508	4	102	as	as	ADP
cet-13508	4	103	saccharomyces	saccharomyce	NOUN
cet-13508	4	104	cerevisiae	cerevisiae	NOUN
cet-13508	4	105	,	,	PUNCT
cet-13508	4	106	or	or	CCONJ
cet-13508	4	107	brewer	brewer	NOUN
cet-13508	4	108	’s	’s	PART
cet-13508	4	109	yeast	yeast	NOUN
cet-13508	4	110	(	(	PUNCT
cet-13508	4	111	bsy	bsy	NOUN
cet-13508	4	112	)	)	PUNCT
cet-13508	4	113	.	.	PUNCT
cet-13508	5	1	the	the	DET
cet-13508	5	2	generation	generation	NOUN
cet-13508	5	3	of	of	ADP
cet-13508	5	4	gnos	gno	NOUN
cet-13508	5	5	from	from	ADP
cet-13508	5	6	bsy	bsy	NOUN
cet-13508	5	7	by	by	ADP
cet-13508	5	8	enzymatic	enzymatic	ADJ
cet-13508	5	9	hydrolysis	hydrolysis	NOUN
cet-13508	5	10	could	could	AUX
cet-13508	5	11	create	create	VERB
cet-13508	5	12	functional	functional	ADJ
cet-13508	5	13	food	food	NOUN
cet-13508	5	14	additives	additive	NOUN
cet-13508	5	15	,	,	PUNCT
cet-13508	5	16	as	as	ADV
cet-13508	5	17	well	well	ADV
cet-13508	5	18	as	as	ADP
cet-13508	5	19	minimize	minimize	VERB
cet-13508	5	20	waste	waste	NOUN
cet-13508	5	21	and	and	CCONJ
cet-13508	5	22	waste	waste	NOUN
cet-13508	5	23	-	-	PUNCT
cet-13508	5	24	disposal	disposal	NOUN
cet-13508	5	25	costs	cost	NOUN
cet-13508	5	26	,	,	PUNCT
cet-13508	5	27	thereby	thereby	ADV
cet-13508	5	28	contributing	contribute	VERB
cet-13508	5	29	to	to	ADP
cet-13508	5	30	the	the	DET
cet-13508	5	31	circular	circular	ADJ
cet-13508	5	32	economy	economy	NOUN
cet-13508	5	33	.	.	PUNCT
cet-13508	6	1	in	in	ADP
cet-13508	6	2	this	this	DET
cet-13508	6	3	study	study	NOUN
cet-13508	6	4	,	,	PUNCT
cet-13508	6	5	a	a	DET
cet-13508	6	6	β-1,6glucanase	β-1,6glucanase	NOUN
cet-13508	6	7	from	from	ADP
cet-13508	6	8	the	the	DET
cet-13508	6	9	mycoparasitic	mycoparasitic	ADJ
cet-13508	6	10	fungus	fungus	PROPN
cet-13508	6	11	trichoderma	trichoderma	PROPN
cet-13508	6	12	virens	viren	NOUN
cet-13508	6	13	was	be	AUX
cet-13508	6	14	cloned	clone	VERB
cet-13508	6	15	and	and	CCONJ
cet-13508	6	16	expressed	express	VERB
cet-13508	6	17	in	in	ADP
cet-13508	6	18	pichia	pichia	NOUN
cet-13508	6	19	pastoris	pastoris	NOUN
cet-13508	6	20	,	,	PUNCT
cet-13508	6	21	and	and	CCONJ
cet-13508	6	22	subsequently	subsequently	ADV
cet-13508	6	23	purified	purified	ADJ
cet-13508	6	24	and	and	CCONJ
cet-13508	6	25	characterized	characterize	VERB
cet-13508	6	26	in	in	SCONJ
cet-13508	6	27	order	order	NOUN
cet-13508	6	28	to	to	PART
cet-13508	6	29	assess	assess	VERB
cet-13508	6	30	its	its	PRON
cet-13508	6	31	suitability	suitability	NOUN
cet-13508	6	32	for	for	ADP
cet-13508	6	33	the	the	DET
cet-13508	6	34	production	production	NOUN
cet-13508	6	35	of	of	ADP
cet-13508	6	36	gnos	gno	NOUN
cet-13508	6	37	from	from	ADP
cet-13508	6	38	bsy	bsy	PROPN
cet-13508	6	39	.	.	PROPN
cet-13508	6	40	tvir30	tvir30	PROPN
cet-13508	6	41	was	be	AUX
cet-13508	6	42	purified	purify	VERB
cet-13508	6	43	by	by	ADP
cet-13508	6	44	imac	imac	NOUN
cet-13508	6	45	to	to	ADP
cet-13508	6	46	a	a	DET
cet-13508	6	47	yield	yield	NOUN
cet-13508	6	48	of	of	ADP
cet-13508	6	49	47.5	47.5	NUM
cet-13508	6	50	%	%	NOUN
cet-13508	6	51	with	with	ADP
cet-13508	6	52	a	a	DET
cet-13508	6	53	purification	purification	NOUN
cet-13508	6	54	factor	factor	NOUN
cet-13508	6	55	of	of	ADP
cet-13508	6	56	5.63	5.63	NUM
cet-13508	6	57	.	.	PUNCT
cet-13508	7	1	the	the	DET
cet-13508	7	2	ph	ph	ADJ
cet-13508	7	3	and	and	CCONJ
cet-13508	7	4	temperature	temperature	NOUN
cet-13508	7	5	optima	optima	NOUN
cet-13508	7	6	of	of	ADP
cet-13508	7	7	tvir30	tvir30	NOUN
cet-13508	7	8	were	be	AUX
cet-13508	7	9	determined	determine	VERB
cet-13508	7	10	to	to	PART
cet-13508	7	11	be	be	AUX
cet-13508	7	12	5.0	5.0	NUM
cet-13508	7	13	and	and	CCONJ
cet-13508	7	14	45oc	45oc	NOUN
cet-13508	7	15	respectively	respectively	ADV
cet-13508	7	16	.	.	PUNCT
cet-13508	8	1	the	the	DET
cet-13508	8	2	enzyme	enzyme	NOUN
cet-13508	8	3	was	be	AUX
cet-13508	8	4	found	find	VERB
cet-13508	8	5	to	to	PART
cet-13508	8	6	be	be	AUX
cet-13508	8	7	glycosylated	glycosylate	VERB
cet-13508	8	8	,	,	PUNCT
cet-13508	8	9	and	and	CCONJ
cet-13508	8	10	substrate	substrate	NOUN
cet-13508	8	11	specificity	specificity	NOUN
cet-13508	8	12	analysis	analysis	NOUN
cet-13508	8	13	revealed	reveal	VERB
cet-13508	8	14	it	it	PRON
cet-13508	8	15	to	to	PART
cet-13508	8	16	be	be	AUX
cet-13508	8	17	specific	specific	ADJ
cet-13508	8	18	to	to	ADP
cet-13508	8	19	β-1,6	β-1,6	NOUN
cet-13508	8	20	-	-	NOUN
cet-13508	8	21	linkages	linkage	NOUN
cet-13508	8	22	only	only	ADV
cet-13508	8	23	.	.	PUNCT
cet-13508	9	1	tvir30	tvir30	PROPN
cet-13508	9	2	was	be	AUX
cet-13508	9	3	relatively	relatively	ADV
cet-13508	9	4	heat	heat	NOUN
cet-13508	9	5	sensitive	sensitive	ADJ
cet-13508	9	6	,	,	PUNCT
cet-13508	9	7	with	with	ADP
cet-13508	9	8	50	50	NUM
cet-13508	9	9	%	%	NOUN
cet-13508	9	10	relative	relative	ADJ
cet-13508	9	11	activity	activity	NOUN
cet-13508	9	12	remaining	remain	VERB
cet-13508	9	13	after	after	ADP
cet-13508	9	14	15	15	NUM
cet-13508	9	15	min	min	NOUN
cet-13508	9	16	incubation	incubation	NOUN
cet-13508	9	17	at	at	ADP
cet-13508	9	18	50oc	50oc	PROPN
cet-13508	9	19	.	.	PUNCT
cet-13508	10	1	however	however	ADV
cet-13508	10	2	,	,	PUNCT
cet-13508	10	3	at	at	ADP
cet-13508	10	4	40oc	40oc	PROPN
cet-13508	10	5	the	the	DET
cet-13508	10	6	stability	stability	NOUN
cet-13508	10	7	of	of	ADP
cet-13508	10	8	tvir30	tvir30	NOUN
cet-13508	10	9	was	be	AUX
cet-13508	10	10	improved	improve	VERB
cet-13508	10	11	,	,	PUNCT
cet-13508	10	12	with	with	ADP
cet-13508	10	13	activity	activity	NOUN
cet-13508	10	14	reaching	reach	VERB
cet-13508	10	15	80	80	NUM
cet-13508	10	16	%	%	NOUN
cet-13508	10	17	at	at	ADP
cet-13508	10	18	100	100	NUM
cet-13508	10	19	h	h	NOUN
cet-13508	10	20	of	of	ADP
cet-13508	10	21	incubation	incubation	NOUN
cet-13508	10	22	.	.	PUNCT
cet-13508	11	1	kinetic	kinetic	ADJ
cet-13508	11	2	analysis	analysis	NOUN
cet-13508	11	3	revealed	reveal	VERB
cet-13508	11	4	classic	classic	ADJ
cet-13508	11	5	michaelis	michaeli	NOUN
cet-13508	11	6	-	-	PUNCT
cet-13508	11	7	menten	menten	ADJ
cet-13508	11	8	kinetics	kinetic	NOUN
cet-13508	11	9	,	,	PUNCT
cet-13508	11	10	with	with	ADP
cet-13508	11	11	a	a	DET
cet-13508	11	12	vmax	vmax	NOUN
cet-13508	11	13	of	of	ADP
cet-13508	11	14	214.4	214.4	NUM
cet-13508	11	15	μmol	μmol	NOUN
cet-13508	11	16	mg-1	mg-1	NOUN
cet-13508	11	17	min-1	min-1	PROPN
cet-13508	11	18	,	,	PUNCT
cet-13508	11	19	a	a	DET
cet-13508	11	20	km	km	NOUN
cet-13508	11	21	of	of	ADP
cet-13508	11	22	1.873	1.873	NUM
cet-13508	11	23	mg	mg	PROPN
cet-13508	11	24	ml-1	ml-1	PROPN
cet-13508	11	25	,	,	PUNCT
cet-13508	11	26	and	and	CCONJ
cet-13508	11	27	a	a	DET
cet-13508	11	28	kcat	kcat	NOUN
cet-13508	11	29	of	of	ADP
cet-13508	11	30	161	161	NUM
cet-13508	11	31	sec-1	sec-1	NOUN
cet-13508	11	32	.	.	PUNCT
cet-13508	12	1	finally	finally	ADV
cet-13508	12	2	,	,	PUNCT
cet-13508	12	3	analysis	analysis	NOUN
cet-13508	12	4	of	of	ADP
cet-13508	12	5	hydrolysis	hydrolysis	NOUN
cet-13508	12	6	products	product	NOUN
cet-13508	12	7	revealed	reveal	VERB
cet-13508	12	8	tvir30	tvir30	NOUN
cet-13508	12	9	to	to	PART
cet-13508	12	10	have	have	VERB
cet-13508	12	11	an	an	DET
cet-13508	12	12	endolytic	endolytic	ADJ
cet-13508	12	13	mode	mode	NOUN
cet-13508	12	14	of	of	ADP
cet-13508	12	15	action	action	NOUN
cet-13508	12	16	,	,	PUNCT
cet-13508	12	17	capable	capable	ADJ
cet-13508	12	18	of	of	ADP
cet-13508	12	19	generating	generate	VERB
cet-13508	12	20	gnos	gno	NOUN
cet-13508	12	21	with	with	ADP
cet-13508	12	22	dp>2	dp>2	NOUN
cet-13508	12	23	,	,	PUNCT
cet-13508	12	24	including	include	VERB
cet-13508	12	25	from	from	ADP
cet-13508	12	26	yeast	yeast	NOUN
cet-13508	12	27	β	β	NOUN
cet-13508	12	28	-	-	NOUN
cet-13508	12	29	glucan	glucan	NOUN
cet-13508	12	30	.	.	PUNCT
cet-13508	13	1	1	1	X
cet-13508	13	2	.	.	X
cet-13508	13	3	introduction	introduction	NOUN
cet-13508	13	4	prebiotics	prebiotic	NOUN
cet-13508	13	5	are	be	AUX
cet-13508	13	6	an	an	DET
cet-13508	13	7	emerging	emerge	VERB
cet-13508	13	8	class	class	NOUN
cet-13508	13	9	of	of	ADP
cet-13508	13	10	health	health	NOUN
cet-13508	13	11	-	-	PUNCT
cet-13508	13	12	promoting	promote	VERB
cet-13508	13	13	food	food	NOUN
cet-13508	13	14	additives	additive	NOUN
cet-13508	13	15	that	that	PRON
cet-13508	13	16	function	function	VERB
cet-13508	13	17	primarily	primarily	ADV
cet-13508	13	18	as	as	ADP
cet-13508	13	19	energy	energy	NOUN
cet-13508	13	20	sources	source	NOUN
cet-13508	13	21	for	for	ADP
cet-13508	13	22	probiotic	probiotic	ADJ
cet-13508	13	23	micro	micro	NOUN
cet-13508	13	24	-	-	NOUN
cet-13508	13	25	organisms	organism	NOUN
cet-13508	13	26	.	.	PUNCT
cet-13508	14	1	the	the	DET
cet-13508	14	2	international	international	ADJ
cet-13508	14	3	scientific	scientific	ADJ
cet-13508	14	4	association	association	NOUN
cet-13508	14	5	for	for	ADP
cet-13508	14	6	probiotics	probiotic	NOUN
cet-13508	14	7	and	and	CCONJ
cet-13508	14	8	prebiotics	prebiotic	NOUN
cet-13508	14	9	(	(	PUNCT
cet-13508	14	10	isapp	isapp	ADJ
cet-13508	14	11	)	)	PUNCT
cet-13508	14	12	defines	define	VERB
cet-13508	14	13	the	the	DET
cet-13508	14	14	term	term	NOUN
cet-13508	14	15	prebiotic	prebiotic	ADJ
cet-13508	14	16	as	as	ADP
cet-13508	14	17	“	"	PUNCT
cet-13508	14	18	a	a	DET
cet-13508	14	19	substrate	substrate	NOUN
cet-13508	14	20	that	that	PRON
cet-13508	14	21	is	be	AUX
cet-13508	14	22	selectively	selectively	ADV
cet-13508	14	23	utilised	utilise	VERB
cet-13508	14	24	by	by	ADP
cet-13508	14	25	host	host	NOUN
cet-13508	14	26	microorganisms	microorganism	NOUN
cet-13508	14	27	conferring	confer	VERB
cet-13508	14	28	a	a	DET
cet-13508	14	29	health	health	NOUN
cet-13508	14	30	benefit	benefit	NOUN
cet-13508	14	31	”	"	PUNCT
cet-13508	14	32	(	(	PUNCT
cet-13508	14	33	gibson	gibson	PROPN
cet-13508	14	34	et	et	PROPN
cet-13508	14	35	al	al	PROPN
cet-13508	14	36	.	.	PROPN
cet-13508	14	37	,	,	PUNCT
cet-13508	14	38	2017	2017	NUM
cet-13508	14	39	)	)	PUNCT
cet-13508	14	40	.	.	PUNCT
cet-13508	15	1	health	health	NOUN
cet-13508	15	2	-	-	PUNCT
cet-13508	15	3	promoting	promote	VERB
cet-13508	15	4	effects	effect	NOUN
cet-13508	15	5	include	include	VERB
cet-13508	15	6	inhibition	inhibition	NOUN
cet-13508	15	7	of	of	ADP
cet-13508	15	8	pathogenic	pathogenic	ADJ
cet-13508	15	9	microorganisms	microorganism	NOUN
cet-13508	15	10	,	,	PUNCT
cet-13508	15	11	production	production	NOUN
cet-13508	15	12	of	of	ADP
cet-13508	15	13	short	short	ADJ
cet-13508	15	14	-	-	PUNCT
cet-13508	15	15	chain	chain	NOUN
cet-13508	15	16	fatty	fatty	NOUN
cet-13508	15	17	acids	acid	NOUN
cet-13508	15	18	,	,	PUNCT
cet-13508	15	19	immune	immune	ADJ
cet-13508	15	20	cell	cell	NOUN
cet-13508	15	21	activation	activation	NOUN
cet-13508	15	22	,	,	PUNCT
cet-13508	15	23	and	and	CCONJ
cet-13508	15	24	even	even	ADV
cet-13508	15	25	anti	anti	ADJ
cet-13508	15	26	-	-	ADJ
cet-13508	15	27	tumorigenic	tumorigenic	ADJ
cet-13508	15	28	activity	activity	NOUN
cet-13508	15	29	(	(	PUNCT
cet-13508	15	30	kim	kim	PROPN
cet-13508	15	31	et	et	PROPN
cet-13508	15	32	al	al	PROPN
cet-13508	15	33	.	.	PROPN
cet-13508	15	34	,	,	PUNCT
cet-13508	15	35	2006	2006	NUM
cet-13508	15	36	)	)	PUNCT
cet-13508	15	37	.	.	PUNCT
cet-13508	16	1	polyand	polyand	NOUN
cet-13508	16	2	oligosaccharides	oligosaccharide	NOUN
cet-13508	16	3	are	be	AUX
cet-13508	16	4	an	an	DET
cet-13508	16	5	important	important	ADJ
cet-13508	16	6	class	class	NOUN
cet-13508	16	7	of	of	ADP
cet-13508	16	8	prebiotics	prebiotic	NOUN
cet-13508	16	9	,	,	PUNCT
cet-13508	16	10	with	with	ADP
cet-13508	16	11	well	well	ADV
cet-13508	16	12	-	-	PUNCT
cet-13508	16	13	characterised	characterise	VERB
cet-13508	16	14	examples	example	NOUN
cet-13508	16	15	such	such	ADJ
cet-13508	16	16	as	as	ADP
cet-13508	16	17	inulin	inulin	NOUN
cet-13508	16	18	,	,	PUNCT
cet-13508	16	19	whereas	whereas	SCONJ
cet-13508	16	20	oligosaccharides	oligosaccharide	NOUN
cet-13508	16	21	derived	derive	VERB
cet-13508	16	22	from	from	ADP
cet-13508	16	23	β-1,6	β-1,6	NOUN
cet-13508	16	24	-	-	PUNCT
cet-13508	16	25	glucan	glucan	PROPN
cet-13508	16	26	are	be	AUX
cet-13508	16	27	considered	consider	VERB
cet-13508	16	28	an	an	DET
cet-13508	16	29	emerging	emerge	VERB
cet-13508	16	30	class	class	NOUN
cet-13508	16	31	(	(	PUNCT
cet-13508	16	32	wang	wang	PROPN
cet-13508	16	33	et	et	PROPN
cet-13508	16	34	al	al	PROPN
cet-13508	16	35	.	.	PROPN
cet-13508	16	36	,	,	PUNCT
cet-13508	16	37	2020	2020	NUM
cet-13508	16	38	)	)	PUNCT
cet-13508	16	39	.	.	PUNCT
cet-13508	17	1	a	a	DET
cet-13508	17	2	polymer	polymer	NOUN
cet-13508	17	3	of	of	ADP
cet-13508	17	4	d	d	NOUN
cet-13508	17	5	-	-	PUNCT
cet-13508	17	6	glucose	glucose	NOUN
cet-13508	17	7	units	unit	NOUN
cet-13508	17	8	joined	join	VERB
cet-13508	17	9	by	by	ADP
cet-13508	17	10	β-1,6	β-1,6	PROPN
cet-13508	17	11	-	-	PUNCT
cet-13508	17	12	branching	branching	NOUN
cet-13508	17	13	,	,	PUNCT
cet-13508	17	14	β-1,6	β-1,6	NOUN
cet-13508	17	15	-	-	PUNCT
cet-13508	17	16	glucan	glucan	PROPN
cet-13508	17	17	is	be	AUX
cet-13508	17	18	found	find	VERB
cet-13508	17	19	within	within	ADP
cet-13508	17	20	the	the	DET
cet-13508	17	21	cell	cell	NOUN
cet-13508	17	22	walls	wall	NOUN
cet-13508	17	23	of	of	ADP
cet-13508	17	24	fungi	fungus	NOUN
cet-13508	17	25	such	such	ADJ
cet-13508	17	26	as	as	ADP
cet-13508	17	27	saccharomyces	saccharomyce	NOUN
cet-13508	17	28	cerevisiae	cerevisiae	NOUN
cet-13508	17	29	,	,	PUNCT
cet-13508	17	30	or	or	CCONJ
cet-13508	17	31	brewer	brewer	NOUN
cet-13508	17	32	’s	’s	PART
cet-13508	17	33	yeast	yeast	NOUN
cet-13508	17	34	,	,	PUNCT
cet-13508	17	35	and	and	CCONJ
cet-13508	17	36	can	can	AUX
cet-13508	17	37	be	be	AUX
cet-13508	17	38	enzymatically	enzymatically	ADV
cet-13508	17	39	digested	digest	VERB
cet-13508	17	40	to	to	PART
cet-13508	17	41	generate	generate	VERB
cet-13508	17	42	short	short	ADJ
cet-13508	17	43	-	-	PUNCT
cet-13508	17	44	chain	chain	NOUN
cet-13508	17	45	oligosaccharides	oligosaccharide	NOUN
cet-13508	17	46	called	call	VERB
cet-13508	17	47	gentio	gentio	NOUN
cet-13508	17	48	-	-	PUNCT
cet-13508	17	49	oligosaccharides	oligosaccharide	NOUN
cet-13508	17	50	(	(	PUNCT
cet-13508	17	51	gnos	gnos	PROPN
cet-13508	17	52	)	)	PUNCT
cet-13508	17	53	.	.	PUNCT
cet-13508	18	1	gnos	gno	NOUN
cet-13508	18	2	have	have	AUX
cet-13508	18	3	been	be	AUX
cet-13508	18	4	found	find	VERB
cet-13508	18	5	to	to	PART
cet-13508	18	6	be	be	AUX
cet-13508	18	7	more	more	ADV
cet-13508	18	8	resistant	resistant	ADJ
cet-13508	18	9	to	to	ADP
cet-13508	18	10	digestion	digestion	NOUN
cet-13508	18	11	than	than	ADP
cet-13508	18	12	oligosaccharides	oligosaccharide	NOUN
cet-13508	18	13	with	with	ADP
cet-13508	18	14	β-1,4or	β-1,4or	NUM
cet-13508	18	15	β-1,3	β-1,3	NOUN
cet-13508	18	16	-	-	PUNCT
cet-13508	18	17	linkages	linkage	NOUN
cet-13508	18	18	,	,	PUNCT
cet-13508	18	19	which	which	PRON
cet-13508	18	20	means	mean	VERB
cet-13508	18	21	they	they	PRON
cet-13508	18	22	may	may	AUX
cet-13508	18	23	be	be	AUX
cet-13508	18	24	able	able	ADJ
cet-13508	18	25	to	to	PART
cet-13508	18	26	reach	reach	VERB
cet-13508	18	27	more	more	ADJ
cet-13508	18	28	distal	distal	ADJ
cet-13508	18	29	parts	part	NOUN
cet-13508	18	30	of	of	ADP
cet-13508	18	31	the	the	DET
cet-13508	18	32	colon	colon	NOUN
cet-13508	18	33	(	(	PUNCT
cet-13508	18	34	ferreira	ferreira	PROPN
cet-13508	18	35	-	-	PUNCT
cet-13508	18	36	lazarte	lazarte	PROPN
cet-13508	18	37	et	et	PROPN
cet-13508	18	38	al	al	PROPN
cet-13508	18	39	.	.	PROPN
cet-13508	18	40	,	,	PUNCT
cet-13508	18	41	2019	2019	NUM
cet-13508	18	42	)	)	PUNCT
cet-13508	18	43	.	.	PUNCT
cet-13508	19	1	as	as	ADP
cet-13508	19	2	such	such	ADJ
cet-13508	19	3	,	,	PUNCT
cet-13508	19	4	spent	spend	VERB
cet-13508	19	5	brewer	brewer	NOUN
cet-13508	19	6	’s	’s	PART
cet-13508	19	7	yeast	yeast	NOUN
cet-13508	19	8	(	(	PUNCT
cet-13508	19	9	bsy	bsy	NOUN
cet-13508	19	10	)	)	PUNCT
cet-13508	19	11	represents	represent	VERB
cet-13508	19	12	a	a	DET
cet-13508	19	13	large	large	ADJ
cet-13508	19	14	untapped	untapped	ADJ
cet-13508	19	15	source	source	NOUN
cet-13508	19	16	of	of	ADP
cet-13508	19	17	lucrative	lucrative	ADJ
cet-13508	19	18	β-1,6	β-1,6	NOUN
cet-13508	19	19	-	-	PUNCT
cet-13508	19	20	glucans	glucan	NOUN
cet-13508	19	21	.	.	PUNCT
cet-13508	20	1	in	in	ADP
cet-13508	20	2	the	the	DET
cet-13508	20	3	production	production	NOUN
cet-13508	20	4	of	of	ADP
cet-13508	20	5	10,000	10,000	NUM
cet-13508	20	6	hl	hl	NOUN
cet-13508	20	7	of	of	ADP
cet-13508	20	8	beer	beer	NOUN
cet-13508	20	9	,	,	PUNCT
cet-13508	20	10	approximately	approximately	ADV
cet-13508	20	11	15	15	NUM
cet-13508	20	12	-	-	SYM
cet-13508	20	13	18	18	NUM
cet-13508	20	14	tons	ton	NOUN
cet-13508	20	15	of	of	ADP
cet-13508	20	16	s.	s.	PROPN
cet-13508	20	17	cerevisiae	cerevisiae	PROPN
cet-13508	20	18	is	be	AUX
cet-13508	20	19	discarded	discard	VERB
cet-13508	20	20	as	as	SCONJ
cet-13508	20	21	brewer	brewer	NOUN
cet-13508	20	22	’s	’s	PART
cet-13508	20	23	spent	spend	VERB
cet-13508	20	24	yeast	yeast	NOUN
cet-13508	20	25	(	(	PUNCT
cet-13508	20	26	bsy	bsy	PROPN
cet-13508	20	27	)	)	PUNCT
cet-13508	20	28	(	(	PUNCT
cet-13508	20	29	kunze	kunze	PROPN
cet-13508	20	30	,	,	PUNCT
cet-13508	20	31	1999	1999	NUM
cet-13508	20	32	)	)	PUNCT
cet-13508	20	33	.	.	PUNCT
cet-13508	21	1	the	the	DET
cet-13508	21	2	yeast	yeast	NOUN
cet-13508	21	3	cell	cell	NOUN
cet-13508	21	4	wall	wall	NOUN
cet-13508	21	5	makes	make	VERB
cet-13508	21	6	up	up	ADP
cet-13508	21	7	20	20	NUM
cet-13508	21	8	-	-	SYM
cet-13508	21	9	30	30	NUM
cet-13508	21	10	%	%	NOUN
cet-13508	21	11	of	of	ADP
cet-13508	21	12	the	the	DET
cet-13508	21	13	total	total	ADJ
cet-13508	21	14	cell	cell	NOUN
cet-13508	21	15	weight	weight	NOUN
cet-13508	21	16	,	,	PUNCT
cet-13508	21	17	approx	approx	PROPN
cet-13508	21	18	.	.	PUNCT
cet-13508	22	1	85	85	NUM
cet-13508	22	2	%	%	NOUN
cet-13508	22	3	of	of	ADP
cet-13508	22	4	the	the	DET
cet-13508	22	5	cell	cell	NOUN
cet-13508	22	6	wall	wall	NOUN
cet-13508	22	7	is	be	AUX
cet-13508	22	8	polysaccharide	polysaccharide	NOUN
cet-13508	22	9	,	,	PUNCT
cet-13508	22	10	and	and	CCONJ
cet-13508	22	11	a	a	DET
cet-13508	22	12	further	further	ADJ
cet-13508	22	13	10	10	NUM
cet-13508	22	14	-	-	SYM
cet-13508	22	15	15	15	NUM
cet-13508	22	16	%	%	NOUN
cet-13508	22	17	of	of	ADP
cet-13508	22	18	that	that	PRON
cet-13508	22	19	is	be	AUX
cet-13508	22	20	β-1,6glucan	β-1,6glucan	ADJ
cet-13508	22	21	(	(	PUNCT
cet-13508	22	22	aimanianda	aimanianda	PROPN
cet-13508	22	23	et	et	PROPN
cet-13508	22	24	al	al	PROPN
cet-13508	22	25	.	.	PROPN
cet-13508	22	26	,	,	PUNCT
cet-13508	22	27	2009	2009	NUM
cet-13508	22	28	)	)	PUNCT
cet-13508	22	29	.	.	PUNCT
cet-13508	23	1	therefore	therefore	ADV
cet-13508	23	2	,	,	PUNCT
cet-13508	23	3	at	at	ADP
cet-13508	23	4	a	a	DET
cet-13508	23	5	conservative	conservative	ADJ
cet-13508	23	6	estimate	estimate	NOUN
cet-13508	23	7	,	,	PUNCT
cet-13508	23	8	the	the	DET
cet-13508	23	9	amount	amount	NOUN
cet-13508	23	10	of	of	ADP
cet-13508	23	11	β-1,6	β-1,6	NOUN
cet-13508	23	12	-	-	PUNCT
cet-13508	23	13	glucan	glucan	NOUN
cet-13508	23	14	present	present	NOUN
cet-13508	23	15	in	in	ADP
cet-13508	23	16	15	15	NUM
cet-13508	23	17	tons	ton	NOUN
cet-13508	23	18	of	of	ADP
cet-13508	23	19	bsy	bsy	NOUN
cet-13508	23	20	is	be	AUX
cet-13508	23	21	~260	~260	NOUN
cet-13508	23	22	kg	kg	X
cet-13508	23	23	.	.	PUNCT
cet-13508	24	1	the	the	DET
cet-13508	24	2	generation	generation	NOUN
cet-13508	24	3	of	of	ADP
cet-13508	24	4	gnos	gno	NOUN
cet-13508	24	5	from	from	ADP
cet-13508	24	6	bsy	bsy	NOUN
cet-13508	24	7	by	by	ADP
cet-13508	24	8	enzymatic	enzymatic	ADJ
cet-13508	24	9	hydrolysis	hydrolysis	NOUN
cet-13508	24	10	could	could	AUX
cet-13508	24	11	create	create	VERB
cet-13508	24	12	functional	functional	ADJ
cet-13508	24	13	food	food	NOUN
cet-13508	24	14	additives	additive	NOUN
cet-13508	24	15	,	,	PUNCT
cet-13508	24	16	as	as	ADV
cet-13508	24	17	well	well	ADV
cet-13508	24	18	as	as	ADP
cet-13508	24	19	minimize	minimize	VERB
cet-13508	24	20	waste	waste	NOUN
cet-13508	24	21	and	and	CCONJ
cet-13508	24	22	waste	waste	NOUN
cet-13508	24	23	-	-	PUNCT
cet-13508	24	24	disposal	disposal	NOUN
cet-13508	24	25	costs	cost	NOUN
cet-13508	24	26	,	,	PUNCT
cet-13508	24	27	thereby	thereby	ADV
cet-13508	24	28	contributing	contribute	VERB
cet-13508	24	29	to	to	ADP
cet-13508	24	30	the	the	DET
cet-13508	24	31	circular	circular	ADJ
cet-13508	24	32	economy	economy	NOUN
cet-13508	24	33	.	.	PUNCT
cet-13508	25	1	in	in	ADP
cet-13508	25	2	this	this	DET
cet-13508	25	3	study	study	NOUN
cet-13508	25	4	,	,	PUNCT
cet-13508	25	5	a	a	DET
cet-13508	25	6	β-1,6	β-1,6	NOUN
cet-13508	25	7	-	-	NOUN
cet-13508	25	8	glucanase	glucanase	NOUN
cet-13508	25	9	from	from	ADP
cet-13508	25	10	the	the	DET
cet-13508	25	11	mycoparasitic	mycoparasitic	ADJ
cet-13508	25	12	fungus	fungus	PROPN
cet-13508	25	13	trichoderma	trichoderma	PROPN
cet-13508	25	14	virens	viren	NOUN
cet-13508	25	15	was	be	AUX
cet-13508	25	16	cloned	clone	VERB
cet-13508	25	17	and	and	CCONJ
cet-13508	25	18	103	103	NUM
cet-13508	25	19	expressed	express	VERB
cet-13508	25	20	in	in	ADP
cet-13508	25	21	pichia	pichia	NOUN
cet-13508	25	22	pastoris	pastoris	NOUN
cet-13508	25	23	,	,	PUNCT
cet-13508	25	24	and	and	CCONJ
cet-13508	25	25	subsequently	subsequently	ADV
cet-13508	25	26	purified	purified	ADJ
cet-13508	25	27	and	and	CCONJ
cet-13508	25	28	characterized	characterize	VERB
cet-13508	25	29	in	in	ADP
cet-13508	25	30	order	order	NOUN
cet-13508	25	31	to	to	PART
cet-13508	25	32	assess	assess	VERB
cet-13508	25	33	its	its	PRON
cet-13508	25	34	suitability	suitability	NOUN
cet-13508	25	35	for	for	ADP
cet-13508	25	36	the	the	DET
cet-13508	25	37	production	production	NOUN
cet-13508	25	38	of	of	ADP
cet-13508	25	39	gnos	gno	NOUN
cet-13508	25	40	from	from	ADP
cet-13508	25	41	bsy	bsy	PROPN
cet-13508	25	42	.	.	PROPN
cet-13508	25	43	2	2	NUM
cet-13508	25	44	.	.	PUNCT
cet-13508	25	45	materials	material	NOUN
cet-13508	25	46	and	and	CCONJ
cet-13508	25	47	methods	method	NOUN
cet-13508	25	48	the	the	DET
cet-13508	25	49	fungal	fungal	ADJ
cet-13508	25	50	β-1,6	β-1,6	ADJ
cet-13508	25	51	-	-	ADJ
cet-13508	25	52	glucanase	glucanase	ADJ
cet-13508	25	53	gene	gene	NOUN
cet-13508	25	54	tvir30	tvir30	NOUN
cet-13508	25	55	from	from	ADP
cet-13508	25	56	trichoderma	trichoderma	NOUN
cet-13508	25	57	virens	viren	NOUN
cet-13508	25	58	was	be	AUX
cet-13508	25	59	transformed	transform	VERB
cet-13508	25	60	into	into	ADP
cet-13508	25	61	pichia	pichia	NOUN
cet-13508	25	62	pastoris	pastoris	NOUN
cet-13508	25	63	via	via	ADP
cet-13508	25	64	electroporation	electroporation	NOUN
cet-13508	25	65	and	and	CCONJ
cet-13508	25	66	the	the	DET
cet-13508	25	67	reaction	reaction	NOUN
cet-13508	25	68	was	be	AUX
cet-13508	25	69	plated	plate	VERB
cet-13508	25	70	on	on	ADP
cet-13508	25	71	ypd	ypd	NOUN
cet-13508	25	72	agar	agar	NOUN
cet-13508	25	73	containing	contain	VERB
cet-13508	25	74	100	100	NUM
cet-13508	25	75	-	-	SYM
cet-13508	25	76	1000	1000	NUM
cet-13508	25	77	μg	μg	PROPN
cet-13508	25	78	ml-1	ml-1	PRON
cet-13508	25	79	zeocin	zeocin	PROPN
cet-13508	25	80	antibiotic	antibiotic	ADJ
cet-13508	25	81	.	.	PUNCT
cet-13508	26	1	resultant	resultant	NOUN
cet-13508	26	2	clones	clone	NOUN
cet-13508	26	3	were	be	AUX
cet-13508	26	4	expressed	express	VERB
cet-13508	26	5	in	in	ADP
cet-13508	26	6	bmgy	bmgy	NOUN
cet-13508	26	7	/	/	SYM
cet-13508	26	8	bmmy	bmmy	ADJ
cet-13508	26	9	media	medium	NOUN
cet-13508	26	10	over	over	ADP
cet-13508	26	11	72	72	NUM
cet-13508	26	12	h	h	NOUN
cet-13508	26	13	in	in	ADP
cet-13508	26	14	2	2	NUM
cet-13508	26	15	ml	ml	NOUN
cet-13508	26	16	cultures	culture	NOUN
cet-13508	26	17	and	and	CCONJ
cet-13508	26	18	screened	screen	VERB
cet-13508	26	19	for	for	ADP
cet-13508	26	20	the	the	DET
cet-13508	26	21	presence	presence	NOUN
cet-13508	26	22	of	of	ADP
cet-13508	26	23	active	active	ADJ
cet-13508	26	24	,	,	PUNCT
cet-13508	26	25	his	his	PRON
cet-13508	26	26	-	-	PUNCT
cet-13508	26	27	tagged	tag	VERB
cet-13508	26	28	protein	protein	NOUN
cet-13508	26	29	.	.	PUNCT
cet-13508	27	1	proteins	protein	NOUN
cet-13508	27	2	were	be	AUX
cet-13508	27	3	visualised	visualise	VERB
cet-13508	27	4	using	use	VERB
cet-13508	27	5	a	a	DET
cet-13508	27	6	10	10	NUM
cet-13508	27	7	%	%	NOUN
cet-13508	27	8	(	(	PUNCT
cet-13508	27	9	w	w	NOUN
cet-13508	27	10	/	/	SYM
cet-13508	27	11	v	v	NOUN
cet-13508	27	12	)	)	PUNCT
cet-13508	27	13	sds	sds	NOUN
cet-13508	27	14	-	-	PUNCT
cet-13508	27	15	page	page	NOUN
cet-13508	27	16	gel	gel	NOUN
cet-13508	27	17	in	in	ADP
cet-13508	27	18	a	a	DET
cet-13508	27	19	vertical	vertical	ADJ
cet-13508	27	20	electrophoretic	electrophoretic	ADJ
cet-13508	27	21	system	system	NOUN
cet-13508	27	22	using	use	VERB
cet-13508	27	23	1x	1x	NUM
cet-13508	27	24	tris	tris	ADJ
cet-13508	27	25	-	-	PUNCT
cet-13508	27	26	glycine	glycine	NOUN
cet-13508	27	27	running	running	NOUN
cet-13508	27	28	buffer	buffer	NOUN
cet-13508	27	29	.	.	PUNCT
cet-13508	28	1	gels	gel	NOUN
cet-13508	28	2	were	be	AUX
cet-13508	28	3	stained	stain	VERB
cet-13508	28	4	using	use	VERB
cet-13508	28	5	a	a	DET
cet-13508	28	6	coomassie	coomassie	ADJ
cet-13508	28	7	stain	stain	NOUN
cet-13508	28	8	for	for	ADP
cet-13508	28	9	1	1	NUM
cet-13508	28	10	hr	hr	NOUN
cet-13508	28	11	at	at	ADP
cet-13508	28	12	rt	rt	PROPN
cet-13508	28	13	and	and	CCONJ
cet-13508	28	14	destained	destain	VERB
cet-13508	28	15	with	with	ADP
cet-13508	28	16	distilled	distil	VERB
cet-13508	28	17	water	water	NOUN
cet-13508	28	18	.	.	PUNCT
cet-13508	29	1	the	the	DET
cet-13508	29	2	highest	high	ADJ
cet-13508	29	3	expressing	express	VERB
cet-13508	29	4	clone	clone	NOUN
cet-13508	29	5	was	be	AUX
cet-13508	29	6	chosen	choose	VERB
cet-13508	29	7	for	for	ADP
cet-13508	29	8	large	large	ADJ
cet-13508	29	9	-	-	PUNCT
cet-13508	29	10	scale	scale	NOUN
cet-13508	29	11	(	(	PUNCT
cet-13508	29	12	150	150	NUM
cet-13508	29	13	ml	ml	NOUN
cet-13508	29	14	)	)	PUNCT
cet-13508	29	15	culture	culture	NOUN
cet-13508	29	16	with	with	ADP
cet-13508	29	17	1	1	NUM
cet-13508	29	18	%	%	NOUN
cet-13508	29	19	methanol	methanol	NOUN
cet-13508	29	20	induction	induction	NOUN
cet-13508	29	21	every	every	DET
cet-13508	29	22	24	24	NUM
cet-13508	29	23	h	h	NOUN
cet-13508	29	24	over	over	ADP
cet-13508	29	25	a	a	DET
cet-13508	29	26	period	period	NOUN
cet-13508	29	27	of	of	ADP
cet-13508	29	28	48	48	NUM
cet-13508	29	29	h.	h.	NOUN
cet-13508	29	30	the	the	DET
cet-13508	29	31	crude	crude	ADJ
cet-13508	29	32	enzyme	enzyme	NOUN
cet-13508	29	33	was	be	AUX
cet-13508	29	34	harvested	harvest	VERB
cet-13508	29	35	by	by	ADP
cet-13508	29	36	centrifugation	centrifugation	NOUN
cet-13508	29	37	,	,	PUNCT
cet-13508	29	38	equilibrated	equilibrate	VERB
cet-13508	29	39	with	with	ADP
cet-13508	29	40	imac	imac	NOUN
cet-13508	29	41	equilibration	equilibration	NOUN
cet-13508	29	42	buffer	buffer	NOUN
cet-13508	29	43	(	(	PUNCT
cet-13508	29	44	50	50	NUM
cet-13508	29	45	mm	mm	NOUN
cet-13508	29	46	sodium	sodium	NOUN
cet-13508	29	47	phosphate	phosphate	NOUN
cet-13508	29	48	,	,	PUNCT
cet-13508	29	49	300	300	NUM
cet-13508	29	50	mm	mm	NOUN
cet-13508	29	51	sodium	sodium	NOUN
cet-13508	29	52	chloride	chloride	NOUN
cet-13508	29	53	;	;	PUNCT
cet-13508	29	54	ph	ph	NOUN
cet-13508	29	55	7.4	7.4	NUM
cet-13508	29	56	)	)	PUNCT
cet-13508	29	57	,	,	PUNCT
cet-13508	29	58	and	and	CCONJ
cet-13508	29	59	applied	apply	VERB
cet-13508	29	60	onto	onto	ADP
cet-13508	29	61	a	a	DET
cet-13508	29	62	hispur	hispur	ADJ
cet-13508	29	63	cobalt	cobalt	NOUN
cet-13508	29	64	resin	resin	NOUN
cet-13508	29	65	packed	pack	VERB
cet-13508	29	66	column	column	NOUN
cet-13508	29	67	.	.	PUNCT
cet-13508	30	1	bound	bind	VERB
cet-13508	30	2	enzyme	enzyme	NOUN
cet-13508	30	3	was	be	AUX
cet-13508	30	4	eluted	elute	VERB
cet-13508	30	5	using	use	VERB
cet-13508	30	6	50	50	NUM
cet-13508	30	7	mm	mm	NUM
cet-13508	30	8	sodium	sodium	NOUN
cet-13508	30	9	phosphate	phosphate	NOUN
cet-13508	30	10	,	,	PUNCT
cet-13508	30	11	300	300	NUM
cet-13508	30	12	mm	mm	NOUN
cet-13508	30	13	sodium	sodium	NOUN
cet-13508	30	14	chloride	chloride	NOUN
cet-13508	30	15	,	,	PUNCT
cet-13508	30	16	150	150	NUM
cet-13508	30	17	mm	mm	NOUN
cet-13508	30	18	imidazole	imidazole	NOUN
cet-13508	30	19	;	;	PUNCT
cet-13508	30	20	ph	ph	NOUN
cet-13508	30	21	7.4	7.4	NUM
cet-13508	30	22	,	,	PUNCT
cet-13508	30	23	diafiltered	diafiltere	VERB
cet-13508	30	24	with	with	ADP
cet-13508	30	25	50	50	NUM
cet-13508	30	26	mm	mm	NOUN
cet-13508	30	27	citrate	citrate	NOUN
cet-13508	30	28	buffer	buffer	NOUN
cet-13508	30	29	ph	ph	VERB
cet-13508	30	30	5.0	5.0	NUM
cet-13508	30	31	,	,	PUNCT
cet-13508	30	32	and	and	CCONJ
cet-13508	30	33	used	use	VERB
cet-13508	30	34	in	in	ADP
cet-13508	30	35	characterisation	characterisation	NOUN
cet-13508	30	36	studies	study	NOUN
cet-13508	30	37	.	.	PUNCT
cet-13508	31	1	each	each	DET
cet-13508	31	2	characterisation	characterisation	NOUN
cet-13508	31	3	was	be	AUX
cet-13508	31	4	performed	perform	VERB
cet-13508	31	5	at	at	ADP
cet-13508	31	6	least	least	ADV
cet-13508	31	7	twice	twice	ADV
cet-13508	31	8	.	.	PUNCT
cet-13508	32	1	enzyme	enzyme	NOUN
cet-13508	32	2	activity	activity	NOUN
cet-13508	32	3	during	during	ADP
cet-13508	32	4	characterisation	characterisation	NOUN
cet-13508	32	5	studies	study	NOUN
cet-13508	32	6	(	(	PUNCT
cet-13508	32	7	optimum	optimum	ADJ
cet-13508	32	8	ph	ph	VERB
cet-13508	32	9	,	,	PUNCT
cet-13508	32	10	temperature	temperature	NOUN
cet-13508	32	11	,	,	PUNCT
cet-13508	32	12	and	and	CCONJ
cet-13508	32	13	substrate	substrate	NOUN
cet-13508	32	14	specificity	specificity	NOUN
cet-13508	32	15	)	)	PUNCT
cet-13508	32	16	was	be	AUX
cet-13508	32	17	assessed	assess	VERB
cet-13508	32	18	by	by	ADP
cet-13508	32	19	the	the	DET
cet-13508	32	20	amount	amount	NOUN
cet-13508	32	21	of	of	ADP
cet-13508	32	22	reducing	reduce	VERB
cet-13508	32	23	sugar	sugar	NOUN
cet-13508	32	24	liberated	liberate	VERB
cet-13508	32	25	during	during	ADP
cet-13508	32	26	the	the	DET
cet-13508	32	27	incubation	incubation	NOUN
cet-13508	32	28	of	of	ADP
cet-13508	32	29	enzyme	enzyme	NOUN
cet-13508	32	30	with	with	ADP
cet-13508	32	31	substrate	substrate	NOUN
cet-13508	32	32	via	via	ADP
cet-13508	32	33	the	the	DET
cet-13508	32	34	3,5	3,5	NUM
cet-13508	32	35	-	-	PUNCT
cet-13508	32	36	dinitrosalicylic	dinitrosalicylic	ADJ
cet-13508	32	37	acid	acid	NOUN
cet-13508	32	38	(	(	PUNCT
cet-13508	32	39	dns	dns	NOUN
cet-13508	32	40	)	)	PUNCT
cet-13508	32	41	assay	assay	PROPN
cet-13508	32	42	(	(	PUNCT
cet-13508	32	43	miller	miller	PROPN
cet-13508	32	44	,	,	PUNCT
cet-13508	32	45	1959	1959	NUM
cet-13508	32	46	)	)	PUNCT
cet-13508	32	47	.	.	PUNCT
cet-13508	33	1	a	a	DET
cet-13508	33	2	standard	standard	ADJ
cet-13508	33	3	assay	assay	NOUN
cet-13508	33	4	involved	involve	VERB
cet-13508	33	5	the	the	DET
cet-13508	33	6	addition	addition	NOUN
cet-13508	33	7	of	of	ADP
cet-13508	33	8	0.25	0.25	NUM
cet-13508	33	9	ml	ml	NOUN
cet-13508	33	10	of	of	ADP
cet-13508	33	11	enzyme	enzyme	NOUN
cet-13508	33	12	to	to	ADP
cet-13508	33	13	0.25	0.25	NUM
cet-13508	33	14	ml	ml	NOUN
cet-13508	33	15	of	of	ADP
cet-13508	33	16	0.5	0.5	NUM
cet-13508	33	17	%	%	NOUN
cet-13508	33	18	(	(	PUNCT
cet-13508	33	19	w	w	NOUN
cet-13508	33	20	/	/	SYM
cet-13508	33	21	v	v	NOUN
cet-13508	33	22	)	)	PUNCT
cet-13508	33	23	substrate	substrate	NOUN
cet-13508	33	24	,	,	PUNCT
cet-13508	33	25	pre	pre	VERB
cet-13508	33	26	-	-	VERB
cet-13508	33	27	equilibrated	equilibrate	VERB
cet-13508	33	28	to	to	ADP
cet-13508	33	29	the	the	DET
cet-13508	33	30	desired	desire	VERB
cet-13508	33	31	temperature	temperature	NOUN
cet-13508	33	32	,	,	PUNCT
cet-13508	33	33	and	and	CCONJ
cet-13508	33	34	incubation	incubation	NOUN
cet-13508	33	35	of	of	ADP
cet-13508	33	36	the	the	DET
cet-13508	33	37	reaction	reaction	NOUN
cet-13508	33	38	at	at	ADP
cet-13508	33	39	that	that	DET
cet-13508	33	40	temperature	temperature	NOUN
cet-13508	33	41	for	for	ADP
cet-13508	33	42	15	15	NUM
cet-13508	33	43	min	min	NOUN
cet-13508	33	44	.	.	PUNCT
cet-13508	34	1	the	the	DET
cet-13508	34	2	reaction	reaction	NOUN
cet-13508	34	3	was	be	AUX
cet-13508	34	4	stopped	stop	VERB
cet-13508	34	5	by	by	ADP
cet-13508	34	6	the	the	DET
cet-13508	34	7	addition	addition	NOUN
cet-13508	34	8	of	of	ADP
cet-13508	34	9	0.75	0.75	NUM
cet-13508	34	10	ml	ml	NOUN
cet-13508	34	11	dns	dns	NOUN
cet-13508	34	12	reagent	reagent	NOUN
cet-13508	34	13	and	and	CCONJ
cet-13508	34	14	heated	heat	VERB
cet-13508	34	15	to	to	ADP
cet-13508	34	16	95oc	95oc	NOUN
cet-13508	34	17	for	for	ADP
cet-13508	34	18	5	5	NUM
cet-13508	34	19	min	min	NOUN
cet-13508	34	20	,	,	PUNCT
cet-13508	34	21	then	then	ADV
cet-13508	34	22	placed	place	VERB
cet-13508	34	23	on	on	ADP
cet-13508	34	24	ice	ice	NOUN
cet-13508	34	25	for	for	ADP
cet-13508	34	26	10	10	NUM
cet-13508	34	27	min	min	NOUN
cet-13508	34	28	.	.	PROPN
cet-13508	35	1	once	once	ADV
cet-13508	35	2	at	at	ADP
cet-13508	35	3	room	room	NOUN
cet-13508	35	4	temperature	temperature	NOUN
cet-13508	35	5	,	,	PUNCT
cet-13508	35	6	the	the	DET
cet-13508	35	7	absorbance	absorbance	NOUN
cet-13508	35	8	of	of	ADP
cet-13508	35	9	the	the	DET
cet-13508	35	10	reaction	reaction	NOUN
cet-13508	35	11	was	be	AUX
cet-13508	35	12	recorded	record	VERB
cet-13508	35	13	at	at	ADP
cet-13508	35	14	540	540	NUM
cet-13508	35	15	nm	nm	NOUN
cet-13508	35	16	using	use	VERB
cet-13508	35	17	a	a	DET
cet-13508	35	18	spectrophotometer	spectrophotometer	NOUN
cet-13508	35	19	.	.	PUNCT
cet-13508	36	1	every	every	DET
cet-13508	36	2	assay	assay	NOUN
cet-13508	36	3	was	be	AUX
cet-13508	36	4	performed	perform	VERB
cet-13508	36	5	in	in	ADP
cet-13508	36	6	triplicate	triplicate	NOUN
cet-13508	36	7	with	with	ADP
cet-13508	36	8	an	an	DET
cet-13508	36	9	appropriate	appropriate	ADJ
cet-13508	36	10	blank	blank	NOUN
cet-13508	36	11	(	(	PUNCT
cet-13508	36	12	n=3	n=3	NOUN
cet-13508	36	13	)	)	PUNCT
cet-13508	36	14	.	.	PUNCT
cet-13508	37	1	the	the	DET
cet-13508	37	2	products	product	NOUN
cet-13508	37	3	of	of	ADP
cet-13508	37	4	hydrolysis	hydrolysis	NOUN
cet-13508	37	5	were	be	AUX
cet-13508	37	6	analysed	analyse	VERB
cet-13508	37	7	by	by	ADP
cet-13508	37	8	high	high	ADJ
cet-13508	37	9	-	-	PUNCT
cet-13508	37	10	performance	performance	NOUN
cet-13508	37	11	anion	anion	NOUN
cet-13508	37	12	-	-	PUNCT
cet-13508	37	13	exchange	exchange	NOUN
cet-13508	37	14	chromatography	chromatography	NOUN
cet-13508	37	15	with	with	ADP
cet-13508	37	16	pulsed	pulse	VERB
cet-13508	37	17	amperometric	amperometric	NOUN
cet-13508	37	18	detection	detection	NOUN
cet-13508	37	19	(	(	PUNCT
cet-13508	37	20	hpae	hpae	NOUN
cet-13508	37	21	-	-	PUNCT
cet-13508	37	22	pad	pad	NOUN
cet-13508	37	23	)	)	PUNCT
cet-13508	37	24	using	use	VERB
cet-13508	37	25	a	a	DET
cet-13508	37	26	carbopac	carbopac	NOUN
cet-13508	37	27	pa200	pa200	PROPN
cet-13508	37	28	column	column	NOUN
cet-13508	37	29	,	,	PUNCT
cet-13508	37	30	90	90	NUM
cet-13508	37	31	%	%	NOUN
cet-13508	37	32	0.1	0.1	NUM
cet-13508	37	33	m	m	NOUN
cet-13508	37	34	naoh	naoh	NOUN
cet-13508	37	35	and	and	CCONJ
cet-13508	37	36	10	10	NUM
cet-13508	37	37	%	%	NOUN
cet-13508	37	38	0.1	0.1	NUM
cet-13508	37	39	m	m	NOUN
cet-13508	37	40	naoh	naoh	NOUN
cet-13508	37	41	in	in	ADP
cet-13508	37	42	0.5	0.5	NUM
cet-13508	37	43	m	m	NOUN
cet-13508	37	44	naoac	naoac	NOUN
cet-13508	37	45	,	,	PUNCT
cet-13508	37	46	at	at	ADP
cet-13508	37	47	30oc	30oc	PROPN
cet-13508	37	48	with	with	ADP
cet-13508	37	49	a	a	DET
cet-13508	37	50	flow	flow	NOUN
cet-13508	37	51	rate	rate	NOUN
cet-13508	37	52	of	of	ADP
cet-13508	37	53	0.35	0.35	NUM
cet-13508	37	54	ml	ml	NOUN
cet-13508	37	55	/	/	SYM
cet-13508	37	56	min	min	NOUN
cet-13508	37	57	,	,	PUNCT
cet-13508	37	58	and	and	CCONJ
cet-13508	37	59	thin	thin	ADJ
cet-13508	37	60	layer	layer	NOUN
cet-13508	37	61	chromatography	chromatography	NOUN
cet-13508	37	62	(	(	PUNCT
cet-13508	37	63	tlc	tlc	NOUN
cet-13508	37	64	)	)	PUNCT
cet-13508	37	65	.	.	PUNCT
cet-13508	38	1	during	during	ADP
cet-13508	38	2	tlc	tlc	PROPN
cet-13508	38	3	,	,	PUNCT
cet-13508	38	4	0.5	0.5	NUM
cet-13508	38	5	ml	ml	NOUN
cet-13508	38	6	of	of	ADP
cet-13508	38	7	1	1	NUM
cet-13508	38	8	%	%	NOUN
cet-13508	38	9	(	(	PUNCT
cet-13508	38	10	w	w	NOUN
cet-13508	38	11	/	/	SYM
cet-13508	38	12	v	v	NOUN
cet-13508	38	13	)	)	PUNCT
cet-13508	38	14	substrate	substrate	NOUN
cet-13508	38	15	was	be	AUX
cet-13508	38	16	incubated	incubate	VERB
cet-13508	38	17	with	with	ADP
cet-13508	38	18	0.5	0.5	NUM
cet-13508	38	19	ml	ml	NOUN
cet-13508	38	20	of	of	ADP
cet-13508	38	21	enzyme	enzyme	NOUN
cet-13508	38	22	at	at	ADP
cet-13508	38	23	specified	specified	ADJ
cet-13508	38	24	reaction	reaction	NOUN
cet-13508	38	25	conditions	condition	NOUN
cet-13508	38	26	.	.	PUNCT
cet-13508	39	1	the	the	DET
cet-13508	39	2	hydrolysis	hydrolysis	NOUN
cet-13508	39	3	reaction	reaction	NOUN
cet-13508	39	4	was	be	AUX
cet-13508	39	5	then	then	ADV
cet-13508	39	6	concentrated	concentrate	VERB
cet-13508	39	7	to	to	ADP
cet-13508	39	8	0.1	0.1	NUM
cet-13508	39	9	ml	ml	NOUN
cet-13508	39	10	by	by	ADP
cet-13508	39	11	evaporation	evaporation	NOUN
cet-13508	39	12	and	and	CCONJ
cet-13508	39	13	0.5	0.5	NUM
cet-13508	39	14	µl	µl	NOUN
cet-13508	39	15	was	be	AUX
cet-13508	39	16	spotted	spot	VERB
cet-13508	39	17	onto	onto	ADP
cet-13508	39	18	silica	silica	NOUN
cet-13508	39	19	-	-	PUNCT
cet-13508	39	20	coated	coat	VERB
cet-13508	39	21	tlc	tlc	NOUN
cet-13508	39	22	plates	plate	NOUN
cet-13508	39	23	which	which	PRON
cet-13508	39	24	were	be	AUX
cet-13508	39	25	placed	place	VERB
cet-13508	39	26	into	into	ADP
cet-13508	39	27	a	a	DET
cet-13508	39	28	chamber	chamber	NOUN
cet-13508	39	29	containing	contain	VERB
cet-13508	39	30	ethyl	ethyl	PROPN
cet-13508	39	31	acetate	acetate	NOUN
cet-13508	39	32	:	:	PUNCT
cet-13508	39	33	acetic	acetic	ADJ
cet-13508	39	34	acid	acid	NOUN
cet-13508	39	35	:	:	PUNCT
cet-13508	39	36	water	water	NOUN
cet-13508	39	37	(	(	PUNCT
cet-13508	39	38	2:2:1	2:2:1	NUM
cet-13508	39	39	)	)	PUNCT
cet-13508	40	1	(	(	PUNCT
cet-13508	40	2	kumar	kumar	PROPN
cet-13508	40	3	et	et	PROPN
cet-13508	40	4	al	al	PROPN
cet-13508	40	5	.	.	PROPN
cet-13508	40	6	,	,	PUNCT
cet-13508	40	7	2020	2020	NUM
cet-13508	40	8	)	)	PUNCT
cet-13508	40	9	.	.	PUNCT
cet-13508	41	1	the	the	DET
cet-13508	41	2	tlc	tlc	NOUN
cet-13508	41	3	plates	plate	NOUN
cet-13508	41	4	were	be	AUX
cet-13508	41	5	developed	develop	VERB
cet-13508	41	6	in	in	ADP
cet-13508	41	7	a	a	DET
cet-13508	41	8	100oc	100oc	NOUN
cet-13508	41	9	oven	oven	VERB
cet-13508	41	10	after	after	ADP
cet-13508	41	11	spraying	spray	VERB
cet-13508	41	12	with	with	ADP
cet-13508	41	13	10	10	NUM
cet-13508	41	14	%	%	NOUN
cet-13508	41	15	(	(	PUNCT
cet-13508	41	16	v	v	NOUN
cet-13508	41	17	/	/	SYM
cet-13508	41	18	v	v	NOUN
cet-13508	41	19	)	)	PUNCT
cet-13508	41	20	sulfuric	sulfuric	NOUN
cet-13508	41	21	acid	acid	NOUN
cet-13508	41	22	.	.	PUNCT
cet-13508	42	1	3	3	X
cet-13508	42	2	.	.	NOUN
cet-13508	42	3	results	result	NOUN
cet-13508	42	4	and	and	CCONJ
cet-13508	42	5	discussion	discussion	NOUN
cet-13508	42	6	a	a	DET
cet-13508	42	7	150	150	NUM
cet-13508	42	8	ml	ml	NOUN
cet-13508	42	9	sample	sample	NOUN
cet-13508	42	10	of	of	ADP
cet-13508	42	11	crude	crude	ADJ
cet-13508	42	12	enzyme	enzyme	NOUN
cet-13508	42	13	was	be	AUX
cet-13508	42	14	collected	collect	VERB
cet-13508	42	15	48	48	NUM
cet-13508	42	16	h	h	NOUN
cet-13508	42	17	post	post	NOUN
cet-13508	42	18	-	-	NOUN
cet-13508	42	19	induction	induction	NOUN
cet-13508	42	20	and	and	CCONJ
cet-13508	42	21	purified	purify	VERB
cet-13508	42	22	by	by	ADP
cet-13508	42	23	imac	imac	NOUN
cet-13508	42	24	.	.	PUNCT
cet-13508	43	1	the	the	DET
cet-13508	43	2	purified	purified	ADJ
cet-13508	43	3	enzyme	enzyme	NOUN
cet-13508	43	4	was	be	AUX
cet-13508	43	5	then	then	ADV
cet-13508	43	6	characterised	characterise	VERB
cet-13508	43	7	to	to	PART
cet-13508	43	8	determine	determine	VERB
cet-13508	43	9	its	its	PRON
cet-13508	43	10	optimum	optimum	ADJ
cet-13508	43	11	temperature	temperature	NOUN
cet-13508	43	12	,	,	PUNCT
cet-13508	43	13	ph	ph	ADJ
cet-13508	43	14	,	,	PUNCT
cet-13508	43	15	substrate	substrate	NOUN
cet-13508	43	16	specificity	specificity	NOUN
cet-13508	43	17	,	,	PUNCT
cet-13508	43	18	thermal	thermal	ADJ
cet-13508	43	19	stability	stability	NOUN
cet-13508	43	20	,	,	PUNCT
cet-13508	43	21	kinetic	kinetic	ADJ
cet-13508	43	22	properties	property	NOUN
cet-13508	43	23	,	,	PUNCT
cet-13508	43	24	and	and	CCONJ
cet-13508	43	25	products	product	NOUN
cet-13508	43	26	of	of	ADP
cet-13508	43	27	substrate	substrate	NOUN
cet-13508	43	28	hydrolysis	hydrolysis	NOUN
cet-13508	43	29	.	.	PUNCT
cet-13508	44	1	3.1	3.1	NUM
cet-13508	44	2	imac	imac	PROPN
cet-13508	44	3	purification	purification	NOUN
cet-13508	44	4	tvir30	tvir30	NOUN
cet-13508	44	5	was	be	AUX
cet-13508	44	6	purified	purify	VERB
cet-13508	44	7	using	use	VERB
cet-13508	44	8	a	a	DET
cet-13508	44	9	one	one	NUM
cet-13508	44	10	-	-	PUNCT
cet-13508	44	11	step	step	NOUN
cet-13508	44	12	imac	imac	NOUN
cet-13508	44	13	technique	technique	NOUN
cet-13508	44	14	using	use	VERB
cet-13508	44	15	hispur	hispur	ADJ
cet-13508	44	16	cobalt	cobalt	NOUN
cet-13508	44	17	resin	resin	NOUN
cet-13508	44	18	.	.	PUNCT
cet-13508	45	1	the	the	DET
cet-13508	45	2	chromatogram	chromatogram	PROPN
cet-13508	45	3	(	(	PUNCT
cet-13508	45	4	figure	figure	NOUN
cet-13508	45	5	1	1	NUM
cet-13508	45	6	)	)	PUNCT
cet-13508	45	7	shows	show	VERB
cet-13508	45	8	that	that	SCONJ
cet-13508	45	9	the	the	DET
cet-13508	45	10	elution	elution	NOUN
cet-13508	45	11	of	of	ADP
cet-13508	45	12	tvir30	tvir30	NOUN
cet-13508	45	13	occurred	occur	VERB
cet-13508	45	14	over	over	ADP
cet-13508	45	15	two	two	NUM
cet-13508	45	16	fractions	fraction	NOUN
cet-13508	45	17	,	,	PUNCT
cet-13508	45	18	as	as	SCONJ
cet-13508	45	19	evidenced	evidence	VERB
cet-13508	45	20	by	by	ADP
cet-13508	45	21	the	the	DET
cet-13508	45	22	peak	peak	NOUN
cet-13508	45	23	in	in	ADP
cet-13508	45	24	absorbance	absorbance	NOUN
cet-13508	45	25	at	at	ADP
cet-13508	45	26	280	280	NUM
cet-13508	45	27	nm	nm	NOUN
cet-13508	45	28	and	and	CCONJ
cet-13508	45	29	the	the	DET
cet-13508	45	30	corresponding	corresponding	ADJ
cet-13508	45	31	peak	peak	NOUN
cet-13508	45	32	in	in	ADP
cet-13508	45	33	β-1,6	β-1,6	NOUN
cet-13508	45	34	-	-	ADJ
cet-13508	45	35	glucanase	glucanase	ADJ
cet-13508	45	36	activity	activity	NOUN
cet-13508	45	37	.	.	PUNCT
cet-13508	46	1	the	the	DET
cet-13508	46	2	protein	protein	NOUN
cet-13508	46	3	-	-	PUNCT
cet-13508	46	4	containing	contain	VERB
cet-13508	46	5	elution	elution	NOUN
cet-13508	46	6	fractions	fraction	NOUN
cet-13508	46	7	show	show	VERB
cet-13508	46	8	a	a	DET
cet-13508	46	9	pattern	pattern	NOUN
cet-13508	46	10	of	of	ADP
cet-13508	46	11	two	two	NUM
cet-13508	46	12	protein	protein	NOUN
cet-13508	46	13	bands	band	NOUN
cet-13508	46	14	in	in	ADP
cet-13508	46	15	the	the	DET
cet-13508	46	16	sds	sds	NOUN
cet-13508	46	17	-	-	PUNCT
cet-13508	46	18	page	page	NOUN
cet-13508	46	19	gel	gel	NOUN
cet-13508	46	20	in	in	ADP
cet-13508	46	21	the	the	DET
cet-13508	46	22	correct	correct	ADJ
cet-13508	46	23	size	size	NOUN
cet-13508	46	24	range	range	NOUN
cet-13508	46	25	for	for	ADP
cet-13508	46	26	tvir30	tvir30	NOUN
cet-13508	46	27	,	,	PUNCT
cet-13508	46	28	which	which	PRON
cet-13508	46	29	has	have	VERB
cet-13508	46	30	a	a	DET
cet-13508	46	31	predicted	predict	VERB
cet-13508	46	32	molecular	molecular	ADJ
cet-13508	46	33	mass	mass	NOUN
cet-13508	46	34	of	of	ADP
cet-13508	46	35	51.7	51.7	NUM
cet-13508	46	36	kda	kda	NOUN
cet-13508	46	37	,	,	PUNCT
cet-13508	46	38	with	with	ADP
cet-13508	46	39	no	no	DET
cet-13508	46	40	contaminants	contaminant	NOUN
cet-13508	46	41	(	(	PUNCT
cet-13508	46	42	figure	figure	NOUN
cet-13508	46	43	1	1	NUM
cet-13508	46	44	b	b	NOUN
cet-13508	46	45	,	,	PUNCT
cet-13508	46	46	lanes	lane	NOUN
cet-13508	46	47	2	2	NUM
cet-13508	46	48	and	and	CCONJ
cet-13508	46	49	3	3	NUM
cet-13508	46	50	)	)	PUNCT
cet-13508	46	51	.	.	PUNCT
cet-13508	47	1	the	the	DET
cet-13508	47	2	final	final	ADJ
cet-13508	47	3	yield	yield	NOUN
cet-13508	47	4	of	of	ADP
cet-13508	47	5	tvir30	tvir30	NOUN
cet-13508	47	6	after	after	ADP
cet-13508	47	7	purification	purification	NOUN
cet-13508	47	8	was	be	AUX
cet-13508	47	9	47.5	47.5	NUM
cet-13508	47	10	%	%	NOUN
cet-13508	47	11	,	,	PUNCT
cet-13508	47	12	with	with	ADP
cet-13508	47	13	a	a	DET
cet-13508	47	14	final	final	ADJ
cet-13508	47	15	specific	specific	ADJ
cet-13508	47	16	activity	activity	NOUN
cet-13508	47	17	of	of	ADP
cet-13508	47	18	47.65	47.65	NUM
cet-13508	47	19	μmol	μmol	NOUN
cet-13508	47	20	min-1.mg-1	min-1.mg-1	NOUN
cet-13508	47	21	and	and	CCONJ
cet-13508	47	22	a	a	DET
cet-13508	47	23	purification	purification	NOUN
cet-13508	47	24	factor	factor	NOUN
cet-13508	47	25	of	of	ADP
cet-13508	47	26	5.63	5.63	NUM
cet-13508	47	27	.	.	PUNCT
cet-13508	48	1	the	the	DET
cet-13508	48	2	banding	banding	NOUN
cet-13508	48	3	pattern	pattern	NOUN
cet-13508	48	4	of	of	ADP
cet-13508	48	5	purified	purified	ADJ
cet-13508	48	6	tvir30	tvir30	NOUN
cet-13508	48	7	can	can	AUX
cet-13508	48	8	be	be	AUX
cet-13508	48	9	explained	explain	VERB
cet-13508	48	10	by	by	ADP
cet-13508	48	11	heterologous	heterologous	ADJ
cet-13508	48	12	glycosylation	glycosylation	NOUN
cet-13508	48	13	.	.	PUNCT
cet-13508	49	1	bioinformatic	bioinformatic	ADJ
cet-13508	49	2	analysis	analysis	NOUN
cet-13508	49	3	of	of	ADP
cet-13508	49	4	tvir30	tvir30	NOUN
cet-13508	49	5	using	use	VERB
cet-13508	49	6	the	the	DET
cet-13508	49	7	eukaryotic	eukaryotic	ADJ
cet-13508	49	8	linear	linear	NOUN
cet-13508	49	9	motif	motif	NOUN
cet-13508	49	10	resource	resource	NOUN
cet-13508	49	11	revealed	reveal	VERB
cet-13508	49	12	two	two	NUM
cet-13508	49	13	potential	potential	ADJ
cet-13508	49	14	n	n	CCONJ
cet-13508	49	15	-	-	PUNCT
cet-13508	49	16	linked	link	VERB
cet-13508	49	17	glycosylation	glycosylation	NOUN
cet-13508	49	18	sites	site	NOUN
cet-13508	49	19	at	at	ADP
cet-13508	49	20	asn140	asn140	PROPN
cet-13508	49	21	and	and	CCONJ
cet-13508	49	22	asn282	asn282	PROPN
cet-13508	49	23	in	in	ADP
cet-13508	49	24	the	the	DET
cet-13508	49	25	amino	amino	NOUN
cet-13508	49	26	acid	acid	NOUN
cet-13508	49	27	sequence	sequence	NOUN
cet-13508	49	28	.	.	PUNCT
cet-13508	50	1	pichia	pichia	NOUN
cet-13508	50	2	pastoris	pastoris	PROPN
cet-13508	50	3	is	be	AUX
cet-13508	50	4	known	know	VERB
cet-13508	50	5	to	to	PART
cet-13508	50	6	glycosylate	glycosylate	VERB
cet-13508	50	7	and	and	CCONJ
cet-13508	50	8	occasionally	occasionally	ADV
cet-13508	50	9	hyperglycosylate	hyperglycosylate	VERB
cet-13508	50	10	recombinant	recombinant	ADJ
cet-13508	50	11	enzymes	enzyme	NOUN
cet-13508	50	12	.	.	PUNCT
cet-13508	51	1	incubation	incubation	NOUN
cet-13508	51	2	of	of	ADP
cet-13508	51	3	tvir30	tvir30	NOUN
cet-13508	51	4	with	with	ADP
cet-13508	51	5	pngase	pngase	PROPN
cet-13508	51	6	f	f	X
cet-13508	51	7	,	,	PUNCT
cet-13508	51	8	an	an	DET
cet-13508	51	9	amidase	amidase	NOUN
cet-13508	51	10	capable	capable	ADJ
cet-13508	51	11	of	of	ADP
cet-13508	51	12	cleaving	cleave	VERB
cet-13508	51	13	most	most	ADJ
cet-13508	51	14	n	n	CCONJ
cet-13508	51	15	-	-	PUNCT
cet-13508	51	16	linked	link	VERB
cet-13508	51	17	oligosaccharides	oligosaccharide	NOUN
cet-13508	51	18	from	from	ADP
cet-13508	51	19	proteins	protein	NOUN
cet-13508	51	20	,	,	PUNCT
cet-13508	51	21	revealed	reveal	VERB
cet-13508	51	22	that	that	SCONJ
cet-13508	51	23	the	the	DET
cet-13508	51	24	heavier	heavy	ADJ
cet-13508	51	25	60	60	NUM
cet-13508	51	26	kda	kda	NOUN
cet-13508	51	27	band	band	NOUN
cet-13508	51	28	was	be	AUX
cet-13508	51	29	a	a	DET
cet-13508	51	30	glycosylated	glycosylated	ADJ
cet-13508	51	31	version	version	NOUN
cet-13508	51	32	of	of	ADP
cet-13508	51	33	the	the	DET
cet-13508	51	34	enzyme	enzyme	NOUN
cet-13508	51	35	,	,	PUNCT
cet-13508	51	36	as	as	SCONJ
cet-13508	51	37	it	it	PRON
cet-13508	51	38	could	could	AUX
cet-13508	51	39	not	not	PART
cet-13508	51	40	be	be	AUX
cet-13508	51	41	resolved	resolve	VERB
cet-13508	51	42	post	post	ADJ
cet-13508	51	43	-	-	NOUN
cet-13508	51	44	digestion	digestion	NOUN
cet-13508	51	45	(	(	PUNCT
cet-13508	51	46	figure	figure	NOUN
cet-13508	51	47	1	1	NUM
cet-13508	51	48	,	,	PUNCT
cet-13508	51	49	lane	lane	NOUN
cet-13508	51	50	4	4	NUM
cet-13508	51	51	)	)	PUNCT
cet-13508	51	52	.	.	PUNCT
cet-13508	52	1	in	in	ADP
cet-13508	52	2	nature	nature	NOUN
cet-13508	52	3	,	,	PUNCT
cet-13508	52	4	the	the	DET
cet-13508	52	5	purpose	purpose	NOUN
cet-13508	52	6	of	of	ADP
cet-13508	52	7	differential	differential	ADJ
cet-13508	52	8	glycosylation	glycosylation	NOUN
cet-13508	52	9	in	in	ADP
cet-13508	52	10	fungi	fungi	PROPN
cet-13508	52	11	is	be	AUX
cet-13508	52	12	to	to	PART
cet-13508	52	13	expand	expand	VERB
cet-13508	52	14	the	the	DET
cet-13508	52	15	diversity	diversity	NOUN
cet-13508	52	16	of	of	ADP
cet-13508	52	17	enzymes	enzyme	NOUN
cet-13508	52	18	secreted	secrete	VERB
cet-13508	52	19	by	by	ADP
cet-13508	52	20	a	a	DET
cet-13508	52	21	particular	particular	ADJ
cet-13508	52	22	strain	strain	NOUN
cet-13508	52	23	.	.	PUNCT
cet-13508	53	1	for	for	ADP
cet-13508	53	2	example	example	NOUN
cet-13508	53	3	,	,	PUNCT
cet-13508	53	4	saccharomyces	saccharomyce	NOUN
cet-13508	53	5	cerevisiae	cerevisiae	NOUN
cet-13508	53	6	was	be	AUX
cet-13508	53	7	shown	show	VERB
cet-13508	53	8	to	to	PART
cet-13508	53	9	produce	produce	VERB
cet-13508	53	10	two	two	NUM
cet-13508	53	11	different	different	ADJ
cet-13508	53	12	exo	exo	NOUN
cet-13508	53	13	-	-	PUNCT
cet-13508	53	14	βglucanases	βglucanase	NOUN
cet-13508	53	15	,	,	PUNCT
cet-13508	53	16	both	both	DET
cet-13508	53	17	products	product	NOUN
cet-13508	53	18	of	of	ADP
cet-13508	53	19	the	the	DET
cet-13508	53	20	same	same	ADJ
cet-13508	53	21	gene	gene	NOUN
cet-13508	53	22	,	,	PUNCT
cet-13508	53	23	with	with	ADP
cet-13508	53	24	different	different	ADJ
cet-13508	53	25	molecular	molecular	ADJ
cet-13508	53	26	masses	masse	NOUN
cet-13508	53	27	and	and	CCONJ
cet-13508	53	28	biochemical	biochemical	ADJ
cet-13508	53	29	properties	property	NOUN
cet-13508	53	30	(	(	PUNCT
cet-13508	53	31	soler	soler	NOUN
cet-13508	53	32	et	et	PROPN
cet-13508	53	33	al	al	PROPN
cet-13508	53	34	.	.	PROPN
cet-13508	53	35	,	,	PUNCT
cet-13508	53	36	1999	1999	NUM
cet-13508	53	37	)	)	PUNCT
cet-13508	53	38	.	.	PUNCT
cet-13508	54	1	it	it	PRON
cet-13508	54	2	was	be	AUX
cet-13508	54	3	proposed	propose	VERB
cet-13508	54	4	that	that	SCONJ
cet-13508	54	5	this	this	DET
cet-13508	54	6	heterogeneity	heterogeneity	NOUN
cet-13508	54	7	arose	arise	VERB
cet-13508	54	8	from	from	ADP
cet-13508	54	9	divergent	divergent	ADJ
cet-13508	54	10	protein	protein	NOUN
cet-13508	54	11	folding	folding	NOUN
cet-13508	54	12	,	,	PUNCT
cet-13508	54	13	which	which	PRON
cet-13508	54	14	could	could	AUX
cet-13508	54	15	in	in	ADP
cet-13508	54	16	turn	turn	NOUN
cet-13508	54	17	mask	mask	VERB
cet-13508	54	18	certain	certain	ADJ
cet-13508	54	19	glycosylation	glycosylation	NOUN
cet-13508	54	20	sites	site	NOUN
cet-13508	54	21	and	and	CCONJ
cet-13508	54	22	alter	alter	VERB
cet-13508	54	23	the	the	DET
cet-13508	54	24	level	level	NOUN
cet-13508	54	25	of	of	ADP
cet-13508	54	26	glycosylation	glycosylation	NOUN
cet-13508	54	27	at	at	ADP
cet-13508	54	28	each	each	DET
cet-13508	54	29	site	site	NOUN
cet-13508	54	30	.	.	PUNCT
cet-13508	55	1	104	104	NUM
cet-13508	55	2	figure	figure	NOUN
cet-13508	55	3	1	1	NUM
cet-13508	55	4	:	:	PUNCT
cet-13508	55	5	a.	a.	NOUN
cet-13508	55	6	chromatogram	chromatogram	PROPN
cet-13508	55	7	of	of	ADP
cet-13508	55	8	tvir30	tvir30	NOUN
cet-13508	55	9	one	one	NUM
cet-13508	55	10	-	-	PUNCT
cet-13508	55	11	step	step	NOUN
cet-13508	55	12	imac	imac	NOUN
cet-13508	55	13	purification	purification	NOUN
cet-13508	55	14	.	.	PUNCT
cet-13508	56	1	absorbance	absorbance	NOUN
cet-13508	56	2	at	at	ADP
cet-13508	56	3	280	280	NUM
cet-13508	56	4	nm	nm	NOUN
cet-13508	56	5	is	be	AUX
cet-13508	56	6	represented	represent	VERB
cet-13508	56	7	by	by	ADP
cet-13508	56	8	blue	blue	ADJ
cet-13508	56	9	points	point	NOUN
cet-13508	56	10	and	and	CCONJ
cet-13508	56	11	enzyme	enzyme	NOUN
cet-13508	56	12	activity	activity	NOUN
cet-13508	56	13	against	against	ADP
cet-13508	56	14	pustulan	pustulan	NOUN
cet-13508	56	15	at	at	ADP
cet-13508	56	16	30oc	30oc	NUM
cet-13508	56	17	,	,	PUNCT
cet-13508	56	18	ph	ph	X
cet-13508	56	19	6.0	6.0	NUM
cet-13508	56	20	by	by	ADP
cet-13508	56	21	orange	orange	PROPN
cet-13508	56	22	points	point	NOUN
cet-13508	56	23	.	.	PUNCT
cet-13508	57	1	b.	b.	NOUN
cet-13508	57	2	10	10	NUM
cet-13508	57	3	%	%	NOUN
cet-13508	57	4	sds	sds	NOUN
cet-13508	57	5	-	-	PUNCT
cet-13508	57	6	page	page	NOUN
cet-13508	57	7	gel	gel	NOUN
cet-13508	57	8	of	of	ADP
cet-13508	57	9	corresponding	correspond	VERB
cet-13508	57	10	tvir30	tvir30	NOUN
cet-13508	57	11	purification	purification	NOUN
cet-13508	57	12	fractions	fraction	NOUN
cet-13508	57	13	and	and	CCONJ
cet-13508	57	14	deglycosylation	deglycosylation	NOUN
cet-13508	57	15	analysis	analysis	NOUN
cet-13508	57	16	.	.	PUNCT
cet-13508	58	1	3.2	3.2	NUM
cet-13508	58	2	determination	determination	NOUN
cet-13508	58	3	of	of	ADP
cet-13508	58	4	temperature	temperature	NOUN
cet-13508	58	5	and	and	CCONJ
cet-13508	58	6	ph	ph	ADJ
cet-13508	58	7	optima	optima	NOUN
cet-13508	58	8	the	the	DET
cet-13508	58	9	optimum	optimum	ADJ
cet-13508	58	10	temperature	temperature	NOUN
cet-13508	58	11	for	for	ADP
cet-13508	58	12	activity	activity	NOUN
cet-13508	58	13	of	of	ADP
cet-13508	58	14	tvir30	tvir30	NOUN
cet-13508	58	15	was	be	AUX
cet-13508	58	16	initially	initially	ADV
cet-13508	58	17	determined	determine	VERB
cet-13508	58	18	in	in	ADP
cet-13508	58	19	50	50	NUM
cet-13508	58	20	mm	mm	NOUN
cet-13508	58	21	potassium	potassium	NOUN
cet-13508	58	22	phosphate	phosphate	NOUN
cet-13508	58	23	buffer	buffer	NOUN
cet-13508	58	24	,	,	PUNCT
cet-13508	58	25	ph	ph	NOUN
cet-13508	58	26	6.0	6.0	NUM
cet-13508	58	27	,	,	PUNCT
cet-13508	58	28	against	against	ADP
cet-13508	58	29	0.5	0.5	NUM
cet-13508	58	30	%	%	NOUN
cet-13508	58	31	(	(	PUNCT
cet-13508	58	32	w	w	NOUN
cet-13508	58	33	/	/	SYM
cet-13508	58	34	v	v	NOUN
cet-13508	58	35	)	)	PUNCT
cet-13508	58	36	pustulan	pustulan	NOUN
cet-13508	58	37	in	in	ADP
cet-13508	58	38	a	a	DET
cet-13508	58	39	standard	standard	NOUN
cet-13508	58	40	assay	assay	NOUN
cet-13508	58	41	.	.	PUNCT
cet-13508	59	1	the	the	DET
cet-13508	59	2	enzyme	enzyme	NOUN
cet-13508	59	3	had	have	VERB
cet-13508	59	4	>	>	X
cet-13508	59	5	80	80	NUM
cet-13508	59	6	%	%	NOUN
cet-13508	59	7	mean	mean	NOUN
cet-13508	59	8	relative	relative	ADJ
cet-13508	59	9	activity	activity	NOUN
cet-13508	59	10	between	between	ADP
cet-13508	59	11	40	40	NUM
cet-13508	59	12	and	and	CCONJ
cet-13508	59	13	50oc	50oc	NOUN
cet-13508	59	14	,	,	PUNCT
cet-13508	59	15	with	with	ADP
cet-13508	59	16	an	an	DET
cet-13508	59	17	optimum	optimum	ADJ
cet-13508	59	18	temperature	temperature	NOUN
cet-13508	59	19	of	of	ADP
cet-13508	59	20	45oc	45oc	NOUN
cet-13508	59	21	(	(	PUNCT
cet-13508	59	22	figure	figure	NOUN
cet-13508	59	23	2	2	NUM
cet-13508	59	24	a	a	NOUN
cet-13508	59	25	)	)	PUNCT
cet-13508	59	26	.	.	PUNCT
cet-13508	60	1	activity	activity	NOUN
cet-13508	60	2	dropped	drop	VERB
cet-13508	60	3	sharply	sharply	ADV
cet-13508	60	4	outside	outside	ADV
cet-13508	60	5	of	of	ADP
cet-13508	60	6	this	this	DET
cet-13508	60	7	range	range	NOUN
cet-13508	60	8	,	,	PUNCT
cet-13508	60	9	and	and	CCONJ
cet-13508	60	10	at	at	ADP
cet-13508	60	11	65oc	65oc	NOUN
cet-13508	60	12	the	the	DET
cet-13508	60	13	enzyme	enzyme	NOUN
cet-13508	60	14	was	be	AUX
cet-13508	60	15	completely	completely	ADV
cet-13508	60	16	deactivated	deactivate	VERB
cet-13508	60	17	,	,	PUNCT
cet-13508	60	18	as	as	SCONJ
cet-13508	60	19	expected	expect	VERB
cet-13508	60	20	for	for	ADP
cet-13508	60	21	an	an	DET
cet-13508	60	22	enzyme	enzyme	NOUN
cet-13508	60	23	from	from	ADP
cet-13508	60	24	a	a	DET
cet-13508	60	25	mesophilic	mesophilic	ADJ
cet-13508	60	26	organism	organism	NOUN
cet-13508	60	27	.	.	PUNCT
cet-13508	61	1	the	the	DET
cet-13508	61	2	optimum	optimum	ADJ
cet-13508	61	3	ph	ph	NOUN
cet-13508	61	4	value	value	NOUN
cet-13508	61	5	of	of	ADP
cet-13508	61	6	tvir30	tvir30	NOUN
cet-13508	61	7	was	be	AUX
cet-13508	61	8	determined	determine	VERB
cet-13508	61	9	in	in	ADP
cet-13508	61	10	100	100	NUM
cet-13508	61	11	mm	mm	NUM
cet-13508	61	12	phosphate	phosphate	ADJ
cet-13508	61	13	-	-	PUNCT
cet-13508	61	14	citrate	citrate	NOUN
cet-13508	61	15	buffer	buffer	NOUN
cet-13508	61	16	,	,	PUNCT
cet-13508	61	17	ph	ph	X
cet-13508	61	18	2.5	2.5	NUM
cet-13508	61	19	–	–	PUNCT
cet-13508	61	20	8	8	NUM
cet-13508	61	21	,	,	PUNCT
cet-13508	61	22	under	under	ADP
cet-13508	61	23	standard	standard	ADJ
cet-13508	61	24	assay	assay	NOUN
cet-13508	61	25	conditions	condition	NOUN
cet-13508	61	26	.	.	PUNCT
cet-13508	62	1	the	the	DET
cet-13508	62	2	enzyme	enzyme	NOUN
cet-13508	62	3	displayed	display	VERB
cet-13508	62	4	>	>	X
cet-13508	62	5	80	80	NUM
cet-13508	62	6	%	%	NOUN
cet-13508	62	7	activity	activity	NOUN
cet-13508	62	8	in	in	ADP
cet-13508	62	9	a	a	DET
cet-13508	62	10	narrow	narrow	ADJ
cet-13508	62	11	ph	ph	ADJ
cet-13508	62	12	range	range	NOUN
cet-13508	62	13	of	of	ADP
cet-13508	62	14	4.5	4.5	NUM
cet-13508	62	15	to	to	PART
cet-13508	62	16	5.5	5.5	NUM
cet-13508	62	17	,	,	PUNCT
cet-13508	62	18	with	with	ADP
cet-13508	62	19	an	an	DET
cet-13508	62	20	optimal	optimal	ADJ
cet-13508	62	21	ph	ph	NOUN
cet-13508	62	22	value	value	NOUN
cet-13508	62	23	of	of	ADP
cet-13508	62	24	5.0	5.0	NUM
cet-13508	62	25	(	(	PUNCT
cet-13508	62	26	figure	figure	NOUN
cet-13508	62	27	2	2	NUM
cet-13508	62	28	b	b	NOUN
cet-13508	62	29	)	)	PUNCT
cet-13508	62	30	.	.	PUNCT
cet-13508	63	1	these	these	DET
cet-13508	63	2	optima	optima	PROPN
cet-13508	63	3	are	be	AUX
cet-13508	63	4	common	common	ADJ
cet-13508	63	5	among	among	ADP
cet-13508	63	6	fungal	fungal	ADJ
cet-13508	63	7	β-1,6	β-1,6	NOUN
cet-13508	63	8	-	-	PUNCT
cet-13508	63	9	glucanases	glucanase	NOUN
cet-13508	63	10	.	.	PUNCT
cet-13508	64	1	for	for	ADP
cet-13508	64	2	example	example	NOUN
cet-13508	64	3	,	,	PUNCT
cet-13508	64	4	bgn16.1	bgn16.1	PROPN
cet-13508	64	5	,	,	PUNCT
cet-13508	64	6	a	a	DET
cet-13508	64	7	glycoside	glycoside	NOUN
cet-13508	64	8	hydrolase	hydrolase	NOUN
cet-13508	64	9	family	family	NOUN
cet-13508	64	10	30	30	NUM
cet-13508	64	11	β-1,6	β-1,6	PROPN
cet-13508	64	12	-	-	PUNCT
cet-13508	64	13	glucanase	glucanase	NOUN
cet-13508	64	14	from	from	ADP
cet-13508	64	15	trichoderma	trichoderma	PROPN
cet-13508	64	16	harzianum	harzianum	NOUN
cet-13508	64	17	,	,	PUNCT
cet-13508	64	18	has	have	VERB
cet-13508	64	19	temperature	temperature	NOUN
cet-13508	64	20	and	and	CCONJ
cet-13508	64	21	ph	ph	ADJ
cet-13508	64	22	optima	optima	NOUN
cet-13508	64	23	of	of	ADP
cet-13508	64	24	50oc	50oc	PROPN
cet-13508	64	25	and	and	CCONJ
cet-13508	64	26	ph	ph	ADJ
cet-13508	64	27	5.5	5.5	NUM
cet-13508	64	28	respectively	respectively	ADV
cet-13508	64	29	(	(	PUNCT
cet-13508	64	30	cruz	cruz	NOUN
cet-13508	64	31	and	and	CCONJ
cet-13508	64	32	llobell	llobell	PROPN
cet-13508	64	33	,	,	PUNCT
cet-13508	64	34	1999	1999	NUM
cet-13508	64	35	)	)	PUNCT
cet-13508	64	36	.	.	PUNCT
cet-13508	65	1	similarly	similarly	ADV
cet-13508	65	2	,	,	PUNCT
cet-13508	65	3	bgn16.3	bgn16.3	NOUN
cet-13508	65	4	,	,	PUNCT
cet-13508	65	5	also	also	ADV
cet-13508	65	6	from	from	ADP
cet-13508	65	7	t.	t.	PROPN
cet-13508	65	8	harzianum	harzianum	PROPN
cet-13508	65	9	(	(	PUNCT
cet-13508	65	10	a	a	DET
cet-13508	65	11	well	well	ADV
cet-13508	65	12	characterised	characterise	VERB
cet-13508	65	13	mycoparasite	mycoparasite	NOUN
cet-13508	65	14	)	)	PUNCT
cet-13508	65	15	,	,	PUNCT
cet-13508	65	16	displays	display	VERB
cet-13508	65	17	optimum	optimum	ADJ
cet-13508	65	18	activity	activity	NOUN
cet-13508	65	19	at	at	ADP
cet-13508	65	20	50oc	50oc	NOUN
cet-13508	65	21	and	and	CCONJ
cet-13508	65	22	ph	ph	ADJ
cet-13508	65	23	5.0	5.0	NUM
cet-13508	65	24	(	(	PUNCT
cet-13508	65	25	montero	montero	PROPN
cet-13508	65	26	et	et	PROPN
cet-13508	65	27	al	al	PROPN
cet-13508	65	28	.	.	PROPN
cet-13508	65	29	,	,	PUNCT
cet-13508	65	30	2005	2005	NUM
cet-13508	65	31	)	)	PUNCT
cet-13508	65	32	.	.	PUNCT
cet-13508	66	1	interestingly	interestingly	ADV
cet-13508	66	2	,	,	PUNCT
cet-13508	66	3	tvir30	tvir30	NOUN
cet-13508	66	4	is	be	AUX
cet-13508	66	5	the	the	DET
cet-13508	66	6	only	only	ADJ
cet-13508	66	7	recombinant	recombinant	ADJ
cet-13508	66	8	fungal	fungal	ADJ
cet-13508	66	9	β-1,6	β-1,6	NOUN
cet-13508	66	10	-	-	NOUN
cet-13508	66	11	glucanase	glucanase	NOUN
cet-13508	66	12	that	that	PRON
cet-13508	66	13	appears	appear	VERB
cet-13508	66	14	to	to	PART
cet-13508	66	15	be	be	AUX
cet-13508	66	16	glycosylated	glycosylate	VERB
cet-13508	66	17	.	.	PUNCT
cet-13508	67	1	volkov	volkov	PROPN
cet-13508	67	2	et	et	PROPN
cet-13508	67	3	al	al	PROPN
cet-13508	67	4	.	.	PROPN
cet-13508	67	5	,	,	PUNCT
cet-13508	67	6	(	(	PUNCT
cet-13508	67	7	2021	2021	NUM
cet-13508	67	8	)	)	PUNCT
cet-13508	67	9	reported	report	VERB
cet-13508	67	10	a	a	DET
cet-13508	67	11	β-1,6glucanase	β-1,6glucanase	NOUN
cet-13508	67	12	from	from	ADP
cet-13508	67	13	t.	t.	PROPN
cet-13508	67	14	harzianum	harzianum	PROPN
cet-13508	67	15	expressed	express	VERB
cet-13508	67	16	in	in	ADP
cet-13508	67	17	penicillium	penicillium	NOUN
cet-13508	67	18	verruculosum	verruculosum	NOUN
cet-13508	67	19	with	with	ADP
cet-13508	67	20	one	one	NUM
cet-13508	67	21	predicted	predict	VERB
cet-13508	67	22	n	n	CCONJ
cet-13508	67	23	-	-	PUNCT
cet-13508	67	24	glycosylation	glycosylation	NOUN
cet-13508	67	25	site	site	NOUN
cet-13508	67	26	but	but	CCONJ
cet-13508	67	27	did	do	AUX
cet-13508	67	28	not	not	PART
cet-13508	67	29	detect	detect	VERB
cet-13508	67	30	any	any	DET
cet-13508	67	31	carbohydrate	carbohydrate	NOUN
cet-13508	67	32	moieties	moiety	NOUN
cet-13508	67	33	within	within	ADP
cet-13508	67	34	the	the	DET
cet-13508	67	35	recombinant	recombinant	ADJ
cet-13508	67	36	protein	protein	NOUN
cet-13508	67	37	(	(	PUNCT
cet-13508	67	38	volkov	volkov	PROPN
cet-13508	67	39	et	et	PROPN
cet-13508	67	40	al	al	PROPN
cet-13508	67	41	.	.	PROPN
cet-13508	67	42	,	,	PUNCT
cet-13508	67	43	2021	2021	NUM
cet-13508	67	44	)	)	PUNCT
cet-13508	67	45	.	.	PUNCT
cet-13508	68	1	figure	figure	VERB
cet-13508	68	2	2	2	NUM
cet-13508	68	3	:	:	PUNCT
cet-13508	68	4	a.	a.	NOUN
cet-13508	68	5	temperature	temperature	NOUN
cet-13508	68	6	profile	profile	NOUN
cet-13508	68	7	of	of	ADP
cet-13508	68	8	purified	purified	ADJ
cet-13508	68	9	tvir30	tvir30	NOUN
cet-13508	68	10	showing	show	VERB
cet-13508	68	11	an	an	DET
cet-13508	68	12	optimum	optimum	ADJ
cet-13508	68	13	temperature	temperature	NOUN
cet-13508	68	14	of	of	ADP
cet-13508	68	15	45oc	45oc	NOUN
cet-13508	68	16	±sd	±sd	NOUN
cet-13508	68	17	,	,	PUNCT
cet-13508	68	18	n	n	NOUN
cet-13508	68	19	=	=	SYM
cet-13508	68	20	3	3	X
cet-13508	68	21	.	.	X
cet-13508	68	22	b.	b.	PROPN
cet-13508	68	23	ph	ph	PROPN
cet-13508	68	24	profile	profile	NOUN
cet-13508	68	25	of	of	ADP
cet-13508	68	26	tvir30	tvir30	NOUN
cet-13508	68	27	,	,	PUNCT
cet-13508	68	28	showing	show	VERB
cet-13508	68	29	optimal	optimal	ADJ
cet-13508	68	30	activity	activity	NOUN
cet-13508	68	31	at	at	ADP
cet-13508	68	32	ph	ph	PROPN
cet-13508	68	33	5.0	5.0	NUM
cet-13508	68	34	±sd	±sd	NUM
cet-13508	68	35	,	,	PUNCT
cet-13508	68	36	n	n	NOUN
cet-13508	68	37	=	=	SYM
cet-13508	68	38	3	3	NUM
cet-13508	68	39	.	.	NOUN
cet-13508	68	40	3.3	3.3	NUM
cet-13508	68	41	substrate	substrate	NOUN
cet-13508	68	42	specificity	specificity	NOUN
cet-13508	68	43	and	and	CCONJ
cet-13508	68	44	thermal	thermal	ADJ
cet-13508	68	45	stability	stability	NOUN
cet-13508	68	46	substrate	substrate	NOUN
cet-13508	68	47	specificity	specificity	NOUN
cet-13508	68	48	analysis	analysis	NOUN
cet-13508	68	49	was	be	AUX
cet-13508	68	50	performed	perform	VERB
cet-13508	68	51	to	to	PART
cet-13508	68	52	confirm	confirm	VERB
cet-13508	68	53	the	the	DET
cet-13508	68	54	activity	activity	NOUN
cet-13508	68	55	of	of	ADP
cet-13508	68	56	tvir30	tvir30	NOUN
cet-13508	68	57	.	.	PUNCT
cet-13508	69	1	results	result	NOUN
cet-13508	69	2	revealed	reveal	VERB
cet-13508	69	3	that	that	SCONJ
cet-13508	69	4	at	at	ADP
cet-13508	69	5	optimum	optimum	ADJ
cet-13508	69	6	assay	assay	NOUN
cet-13508	69	7	conditions	condition	NOUN
cet-13508	69	8	of	of	ADP
cet-13508	69	9	ph	ph	ADJ
cet-13508	69	10	5.0	5.0	NUM
cet-13508	69	11	and	and	CCONJ
cet-13508	69	12	45oc	45oc	NOUN
cet-13508	69	13	,	,	PUNCT
cet-13508	69	14	tvir30	tvir30	NOUN
cet-13508	69	15	had	have	VERB
cet-13508	69	16	the	the	DET
cet-13508	69	17	highest	high	ADJ
cet-13508	69	18	activity	activity	NOUN
cet-13508	69	19	on	on	ADP
cet-13508	69	20	pustulan	pustulan	NOUN
cet-13508	69	21	,	,	PUNCT
cet-13508	69	22	a	a	DET
cet-13508	69	23	polysaccharide	polysaccharide	NOUN
cet-13508	69	24	of	of	ADP
cet-13508	69	25	d	d	NOUN
cet-13508	69	26	-	-	PUNCT
cet-13508	69	27	glucose	glucose	NOUN
cet-13508	69	28	units	unit	NOUN
cet-13508	69	29	joined	join	VERB
cet-13508	69	30	by	by	ADP
cet-13508	69	31	β-1,6	β-1,6	PROPN
cet-13508	69	32	-	-	PUNCT
cet-13508	69	33	branching	branching	NOUN
cet-13508	69	34	.	.	PUNCT
cet-13508	70	1	as	as	SCONJ
cet-13508	70	2	expected	expect	VERB
cet-13508	70	3	,	,	PUNCT
cet-13508	70	4	the	the	DET
cet-13508	70	5	enzyme	enzyme	NOUN
cet-13508	70	6	had	have	VERB
cet-13508	70	7	negligible	negligible	ADJ
cet-13508	70	8	activity	activity	NOUN
cet-13508	70	9	on	on	ADP
cet-13508	70	10	β-1,3and	β-1,3and	NOUN
cet-13508	70	11	β-1,4	β-1,4	ADV
cet-13508	70	12	-	-	PUNCT
cet-13508	70	13	linked	link	VERB
cet-13508	70	14	substrates	substrate	NOUN
cet-13508	70	15	,	,	PUNCT
cet-13508	70	16	such	such	ADJ
cet-13508	70	17	as	as	ADP
cet-13508	70	18	curdlan	curdlan	NOUN
cet-13508	70	19	and	and	CCONJ
cet-13508	70	20	cellulose	cellulose	NOUN
cet-13508	70	21	.	.	PUNCT
cet-13508	71	1	it	it	PRON
cet-13508	71	2	did	do	AUX
cet-13508	71	3	however	however	ADV
cet-13508	71	4	exhibit	exhibit	VERB
cet-13508	71	5	11.9	11.9	NUM
cet-13508	71	6	%	%	NOUN
cet-13508	71	7	activity	activity	NOUN
cet-13508	71	8	on	on	ADP
cet-13508	71	9	laminarin	laminarin	NOUN
cet-13508	71	10	,	,	PUNCT
cet-13508	71	11	a	a	DET
cet-13508	71	12	β-1,3	β-1,3	PROPN
cet-13508	71	13	-	-	PUNCT
cet-13508	71	14	linked	link	VERB
cet-13508	71	15	polysaccharide	polysaccharide	NOUN
cet-13508	71	16	with	with	ADP
cet-13508	71	17	β-1,6	β-1,6	NOUN
cet-13508	71	18	-	-	PUNCT
cet-13508	71	19	branching	branch	VERB
cet-13508	71	20	(	(	PUNCT
cet-13508	71	21	figure	figure	NOUN
cet-13508	71	22	3	3	NUM
cet-13508	71	23	)	)	PUNCT
cet-13508	71	24	.	.	PUNCT
cet-13508	72	1	these	these	DET
cet-13508	72	2	results	result	NOUN
cet-13508	72	3	confirm	confirm	VERB
cet-13508	72	4	tvir30	tvir30	NOUN
cet-13508	72	5	is	be	AUX
cet-13508	72	6	specific	specific	ADJ
cet-13508	72	7	to	to	ADP
cet-13508	72	8	β-1,6	β-1,6	NOUN
cet-13508	72	9	-	-	NOUN
cet-13508	72	10	linkages	linkage	NOUN
cet-13508	72	11	only	only	ADV
cet-13508	72	12	.	.	PUNCT
cet-13508	73	1	0.0	0.0	NUM
cet-13508	73	2	20.0	20.0	NUM
cet-13508	73	3	40.0	40.0	NUM
cet-13508	73	4	60.0	60.0	NUM
cet-13508	73	5	80.0	80.0	NUM
cet-13508	73	6	100.0	100.0	NUM
cet-13508	73	7	0	0	NUM
cet-13508	73	8	2	2	NUM
cet-13508	73	9	4	4	NUM
cet-13508	73	10	6	6	NUM
cet-13508	73	11	8	8	NUM
cet-13508	73	12	10	10	NUM
cet-13508	73	13	ph	ph	NOUN
cet-13508	73	14	0.0	0.0	NUM
cet-13508	73	15	20.0	20.0	NUM
cet-13508	73	16	40.0	40.0	NUM
cet-13508	73	17	60.0	60.0	NUM
cet-13508	73	18	80.0	80.0	NUM
cet-13508	73	19	100.0	100.0	NUM
cet-13508	73	20	0	0	NUM
cet-13508	73	21	20	20	NUM
cet-13508	73	22	40	40	NUM
cet-13508	73	23	60	60	NUM
cet-13508	73	24	80	80	NUM
cet-13508	73	25	m	m	NOUN
cet-13508	73	26	e	e	NOUN
cet-13508	73	27	a	a	DET
cet-13508	73	28	n	n	NOUN
cet-13508	73	29	r	r	NOUN
cet-13508	73	30	e	e	X
cet-13508	73	31	la	la	X
cet-13508	73	32	ti	ti	X
cet-13508	73	33	v	v	ADP
cet-13508	73	34	e	e	X
cet-13508	73	35	e	e	X
cet-13508	73	36	n	n	PROPN
cet-13508	73	37	z	z	PROPN
cet-13508	73	38	y	y	NOUN
cet-13508	73	39	m	m	VERB
cet-13508	73	40	e	e	NOUN
cet-13508	73	41	a	a	PRON
cet-13508	73	42	c	c	NOUN
cet-13508	73	43	ti	ti	NOUN
cet-13508	73	44	v	v	ADP
cet-13508	73	45	it	it	PRON
cet-13508	73	46	y	y	NOUN
cet-13508	73	47	(	(	PUNCT
cet-13508	73	48	%	%	NOUN
cet-13508	73	49	)	)	PUNCT
cet-13508	73	50	temperature	temperature	NOUN
cet-13508	73	51	(	(	PUNCT
cet-13508	73	52	oc	oc	NOUN
cet-13508	73	53	)	)	PUNCT
cet-13508	73	54	a.	a.	PROPN
cet-13508	73	55	b.	b.	PROPN
cet-13508	74	1	105	105	NUM
cet-13508	74	2	figure	figure	NOUN
cet-13508	74	3	3	3	NUM
cet-13508	74	4	:	:	PUNCT
cet-13508	74	5	a.	a.	NOUN
cet-13508	74	6	substrate	substrate	NOUN
cet-13508	74	7	specificity	specificity	NOUN
cet-13508	74	8	of	of	ADP
cet-13508	74	9	tvir30	tvir30	NOUN
cet-13508	74	10	at	at	ADP
cet-13508	74	11	50oc	50oc	PROPN
cet-13508	74	12	,	,	PUNCT
cet-13508	74	13	ph	ph	ADJ
cet-13508	74	14	5.0	5.0	NUM
cet-13508	74	15	,	,	PUNCT
cet-13508	74	16	on	on	ADP
cet-13508	74	17	0.5	0.5	NUM
cet-13508	74	18	%	%	NOUN
cet-13508	74	19	(	(	PUNCT
cet-13508	74	20	w	w	NOUN
cet-13508	74	21	/	/	SYM
cet-13508	74	22	v	v	NOUN
cet-13508	74	23	)	)	PUNCT
cet-13508	74	24	substrate	substrate	NOUN
cet-13508	74	25	.	.	PUNCT
cet-13508	75	1	b.	b.	PROPN
cet-13508	75	2	thermal	thermal	ADJ
cet-13508	75	3	stability	stability	NOUN
cet-13508	75	4	of	of	ADP
cet-13508	75	5	tvir30	tvir30	NOUN
cet-13508	75	6	assessed	assess	VERB
cet-13508	75	7	at	at	ADP
cet-13508	75	8	50oc	50oc	PROPN
cet-13508	75	9	in	in	ADP
cet-13508	75	10	100	100	NUM
cet-13508	75	11	mm	mm	NUM
cet-13508	75	12	phosphate	phosphate	ADJ
cet-13508	75	13	-	-	PUNCT
cet-13508	75	14	citrate	citrate	NOUN
cet-13508	75	15	buffer	buffer	NOUN
cet-13508	75	16	ph	ph	VERB
cet-13508	75	17	5.0	5.0	NUM
cet-13508	75	18	over	over	ADP
cet-13508	75	19	1	1	NUM
cet-13508	75	20	hour	hour	NOUN
cet-13508	75	21	and	and	CCONJ
cet-13508	75	22	c.	c.	NOUN
cet-13508	75	23	40oc	40oc	PROPN
cet-13508	75	24	over	over	ADP
cet-13508	75	25	100	100	NUM
cet-13508	75	26	hr	hr	NOUN
cet-13508	75	27	.	.	PUNCT
cet-13508	76	1	the	the	DET
cet-13508	76	2	thermal	thermal	ADJ
cet-13508	76	3	stability	stability	NOUN
cet-13508	76	4	of	of	ADP
cet-13508	76	5	tvir30	tvir30	NOUN
cet-13508	76	6	was	be	AUX
cet-13508	76	7	assessed	assess	VERB
cet-13508	76	8	by	by	ADP
cet-13508	76	9	incubation	incubation	NOUN
cet-13508	76	10	at	at	ADP
cet-13508	76	11	50oc	50oc	PROPN
cet-13508	76	12	in	in	ADP
cet-13508	76	13	100	100	NUM
cet-13508	76	14	mm	mm	NUM
cet-13508	76	15	phosphate	phosphate	ADJ
cet-13508	76	16	-	-	PUNCT
cet-13508	76	17	citrate	citrate	NOUN
cet-13508	76	18	buffer	buffer	NOUN
cet-13508	76	19	ph	ph	VERB
cet-13508	76	20	5.0	5.0	NUM
cet-13508	76	21	over	over	ADP
cet-13508	76	22	1	1	NUM
cet-13508	76	23	hour	hour	NOUN
cet-13508	76	24	.	.	PUNCT
cet-13508	77	1	it	it	PRON
cet-13508	77	2	was	be	AUX
cet-13508	77	3	revealed	reveal	VERB
cet-13508	77	4	that	that	SCONJ
cet-13508	77	5	at	at	ADP
cet-13508	77	6	15	15	NUM
cet-13508	77	7	min	min	NOUN
cet-13508	77	8	of	of	ADP
cet-13508	77	9	incubation	incubation	NOUN
cet-13508	77	10	at	at	ADP
cet-13508	77	11	50oc	50oc	PROPN
cet-13508	77	12	,	,	PUNCT
cet-13508	77	13	the	the	DET
cet-13508	77	14	enzyme	enzyme	NOUN
cet-13508	77	15	retained	retain	VERB
cet-13508	77	16	50	50	NUM
cet-13508	77	17	%	%	NOUN
cet-13508	77	18	of	of	ADP
cet-13508	77	19	its	its	PRON
cet-13508	77	20	original	original	ADJ
cet-13508	77	21	activity	activity	NOUN
cet-13508	77	22	under	under	ADP
cet-13508	77	23	standard	standard	ADJ
cet-13508	77	24	assay	assay	NOUN
cet-13508	77	25	conditions	condition	NOUN
cet-13508	77	26	(	(	PUNCT
cet-13508	77	27	figure	figure	NOUN
cet-13508	77	28	3b	3b	NUM
cet-13508	77	29	)	)	PUNCT
cet-13508	77	30	.	.	PUNCT
cet-13508	78	1	the	the	DET
cet-13508	78	2	d	d	NOUN
cet-13508	78	3	-	-	NOUN
cet-13508	78	4	value	value	NOUN
cet-13508	78	5	,	,	PUNCT
cet-13508	78	6	i.e.	i.e.	X
cet-13508	78	7	,	,	PUNCT
cet-13508	78	8	the	the	DET
cet-13508	78	9	time	time	NOUN
cet-13508	78	10	it	it	PRON
cet-13508	78	11	takes	take	VERB
cet-13508	78	12	for	for	SCONJ
cet-13508	78	13	the	the	DET
cet-13508	78	14	enzyme	enzyme	NOUN
cet-13508	78	15	to	to	PART
cet-13508	78	16	be	be	AUX
cet-13508	78	17	reduced	reduce	VERB
cet-13508	78	18	to	to	ADP
cet-13508	78	19	10	10	NUM
cet-13508	78	20	%	%	NOUN
cet-13508	78	21	of	of	ADP
cet-13508	78	22	its	its	PRON
cet-13508	78	23	original	original	ADJ
cet-13508	78	24	activity	activity	NOUN
cet-13508	78	25	,	,	PUNCT
cet-13508	78	26	was	be	AUX
cet-13508	78	27	calculated	calculate	VERB
cet-13508	78	28	to	to	PART
cet-13508	78	29	be	be	AUX
cet-13508	78	30	18.4	18.4	NUM
cet-13508	78	31	min	min	NOUN
cet-13508	78	32	.	.	PUNCT
cet-13508	79	1	this	this	DET
cet-13508	79	2	small	small	ADJ
cet-13508	79	3	d	d	NOUN
cet-13508	79	4	-	-	PUNCT
cet-13508	79	5	value	value	NOUN
cet-13508	79	6	indicates	indicate	VERB
cet-13508	79	7	that	that	DET
cet-13508	79	8	tvir30	tvir30	NOUN
cet-13508	79	9	is	be	AUX
cet-13508	79	10	highly	highly	ADV
cet-13508	79	11	liable	liable	ADJ
cet-13508	79	12	to	to	PART
cet-13508	79	13	heat	heat	VERB
cet-13508	79	14	inactivation	inactivation	NOUN
cet-13508	79	15	at	at	ADP
cet-13508	79	16	50oc	50oc	PROPN
cet-13508	79	17	.	.	PUNCT
cet-13508	80	1	conversely	conversely	ADV
cet-13508	80	2	,	,	PUNCT
cet-13508	80	3	at	at	ADP
cet-13508	80	4	40oc	40oc	ADJ
cet-13508	80	5	the	the	DET
cet-13508	80	6	activity	activity	NOUN
cet-13508	80	7	of	of	ADP
cet-13508	80	8	tvir30	tvir30	NOUN
cet-13508	80	9	dropped	drop	VERB
cet-13508	80	10	below	below	ADP
cet-13508	80	11	90	90	NUM
cet-13508	80	12	%	%	NOUN
cet-13508	80	13	after	after	ADP
cet-13508	80	14	72	72	NUM
cet-13508	80	15	h	h	NOUN
cet-13508	80	16	of	of	ADP
cet-13508	80	17	incubation	incubation	NOUN
cet-13508	80	18	only	only	ADV
cet-13508	80	19	(	(	PUNCT
cet-13508	80	20	figure	figure	NOUN
cet-13508	80	21	3c	3c	NUM
cet-13508	80	22	)	)	PUNCT
cet-13508	80	23	.	.	PUNCT
cet-13508	81	1	for	for	SCONJ
cet-13508	81	2	the	the	DET
cet-13508	81	3	enzyme	enzyme	NOUN
cet-13508	81	4	to	to	PART
cet-13508	81	5	effectively	effectively	ADV
cet-13508	81	6	hydrolyse	hydrolyse	VERB
cet-13508	81	7	yeast	yeast	NOUN
cet-13508	81	8	cell	cell	NOUN
cet-13508	81	9	walls	wall	NOUN
cet-13508	81	10	,	,	PUNCT
cet-13508	81	11	the	the	DET
cet-13508	81	12	reaction	reaction	NOUN
cet-13508	81	13	temperature	temperature	NOUN
cet-13508	81	14	must	must	AUX
cet-13508	81	15	be	be	AUX
cet-13508	81	16	below	below	ADP
cet-13508	81	17	50oc	50oc	PROPN
cet-13508	81	18	,	,	PUNCT
cet-13508	81	19	and	and	CCONJ
cet-13508	81	20	40oc	40oc	PROPN
cet-13508	81	21	is	be	AUX
cet-13508	81	22	suitable	suitable	ADJ
cet-13508	81	23	as	as	SCONJ
cet-13508	81	24	the	the	DET
cet-13508	81	25	enzyme	enzyme	NOUN
cet-13508	81	26	maintains	maintain	VERB
cet-13508	81	27	high	high	ADJ
cet-13508	81	28	activity	activity	NOUN
cet-13508	81	29	for	for	ADP
cet-13508	81	30	a	a	DET
cet-13508	81	31	period	period	NOUN
cet-13508	81	32	of	of	ADP
cet-13508	81	33	~	~	PUNCT
cet-13508	81	34	72	72	NUM
cet-13508	81	35	h	h	NOUN
cet-13508	81	36	at	at	ADP
cet-13508	81	37	this	this	DET
cet-13508	81	38	temperature	temperature	NOUN
cet-13508	81	39	.	.	PUNCT
cet-13508	82	1	3.4	3.4	NUM
cet-13508	82	2	enzyme	enzyme	NOUN
cet-13508	82	3	kinetics	kinetic	NOUN
cet-13508	82	4	assessment	assessment	NOUN
cet-13508	82	5	of	of	ADP
cet-13508	82	6	tvir30	tvir30	ADJ
cet-13508	82	7	kinetics	kinetic	NOUN
cet-13508	82	8	was	be	AUX
cet-13508	82	9	carried	carry	VERB
cet-13508	82	10	out	out	ADP
cet-13508	82	11	on	on	ADP
cet-13508	82	12	pustulan	pustulan	NOUN
cet-13508	82	13	at	at	ADP
cet-13508	82	14	a	a	DET
cet-13508	82	15	range	range	NOUN
cet-13508	82	16	of	of	ADP
cet-13508	82	17	concentrations	concentration	NOUN
cet-13508	82	18	from	from	ADP
cet-13508	82	19	0.5	0.5	NUM
cet-13508	82	20	to	to	PART
cet-13508	82	21	10	10	NUM
cet-13508	82	22	mg	mg	NUM
cet-13508	82	23	ml-1	ml-1	ADV
cet-13508	82	24	via	via	ADP
cet-13508	82	25	the	the	DET
cet-13508	82	26	dns	dns	NOUN
cet-13508	82	27	assay	assay	VERB
cet-13508	82	28	,	,	PUNCT
cet-13508	82	29	as	as	SCONJ
cet-13508	82	30	described	describe	VERB
cet-13508	82	31	in	in	ADP
cet-13508	82	32	the	the	DET
cet-13508	82	33	materials	material	NOUN
cet-13508	82	34	and	and	CCONJ
cet-13508	82	35	methods	method	NOUN
cet-13508	82	36	section	section	NOUN
cet-13508	82	37	.	.	PUNCT
cet-13508	83	1	the	the	DET
cet-13508	83	2	assay	assay	NOUN
cet-13508	83	3	was	be	AUX
cet-13508	83	4	performed	perform	VERB
cet-13508	83	5	at	at	ADP
cet-13508	83	6	optimal	optimal	ADJ
cet-13508	83	7	conditions	condition	NOUN
cet-13508	83	8	over	over	ADP
cet-13508	83	9	10	10	NUM
cet-13508	83	10	minutes	minute	NOUN
cet-13508	83	11	,	,	PUNCT
cet-13508	83	12	during	during	ADP
cet-13508	83	13	which	which	PRON
cet-13508	83	14	the	the	DET
cet-13508	83	15	hydrolysis	hydrolysis	NOUN
cet-13508	83	16	reaction	reaction	NOUN
cet-13508	83	17	was	be	AUX
cet-13508	83	18	in	in	ADP
cet-13508	83	19	the	the	DET
cet-13508	83	20	linear	linear	ADJ
cet-13508	83	21	phase	phase	NOUN
cet-13508	83	22	.	.	PUNCT
cet-13508	84	1	gprahpad	gprahpad	PROPN
cet-13508	84	2	prism	prism	NOUN
cet-13508	84	3	9	9	NUM
cet-13508	84	4	was	be	AUX
cet-13508	84	5	used	use	VERB
cet-13508	84	6	to	to	PART
cet-13508	84	7	plot	plot	VERB
cet-13508	84	8	the	the	DET
cet-13508	84	9	reaction	reaction	NOUN
cet-13508	84	10	and	and	CCONJ
cet-13508	84	11	to	to	PART
cet-13508	84	12	determine	determine	VERB
cet-13508	84	13	the	the	DET
cet-13508	84	14	kinetic	kinetic	ADJ
cet-13508	84	15	parameters	parameter	NOUN
cet-13508	84	16	for	for	ADP
cet-13508	84	17	tvir30	tvir30	NOUN
cet-13508	84	18	.	.	PUNCT
cet-13508	85	1	the	the	DET
cet-13508	85	2	michaelis	michaelis	ADJ
cet-13508	85	3	-	-	PUNCT
cet-13508	85	4	menten	menten	ADJ
cet-13508	85	5	constant	constant	ADJ
cet-13508	85	6	(	(	PUNCT
cet-13508	85	7	km	km	NOUN
cet-13508	85	8	)	)	PUNCT
cet-13508	85	9	was	be	AUX
cet-13508	85	10	determined	determine	VERB
cet-13508	85	11	to	to	PART
cet-13508	85	12	be	be	AUX
cet-13508	85	13	1.87	1.87	NUM
cet-13508	85	14	mg	mg	NUM
cet-13508	85	15	ml-1	ml-1	SYM
cet-13508	85	16	,	,	PUNCT
cet-13508	85	17	the	the	DET
cet-13508	85	18	maximal	maximal	ADJ
cet-13508	85	19	velocity	velocity	NOUN
cet-13508	85	20	(	(	PUNCT
cet-13508	85	21	vmax	vmax	PROPN
cet-13508	85	22	)	)	PUNCT
cet-13508	85	23	was	be	AUX
cet-13508	85	24	calculated	calculate	VERB
cet-13508	85	25	as	as	ADP
cet-13508	85	26	214.4	214.4	NUM
cet-13508	85	27	μmol	μmol	NOUN
cet-13508	85	28	min-1.mg-1	min-1.mg-1	NOUN
cet-13508	85	29	,	,	PUNCT
cet-13508	85	30	and	and	CCONJ
cet-13508	85	31	kcat	kcat	NOUN
cet-13508	85	32	as	as	ADP
cet-13508	85	33	161	161	NUM
cet-13508	85	34	s-1	s-1	NOUN
cet-13508	85	35	,	,	PUNCT
cet-13508	85	36	indicating	indicate	VERB
cet-13508	85	37	that	that	SCONJ
cet-13508	85	38	a	a	DET
cet-13508	85	39	single	single	ADJ
cet-13508	85	40	tvir30	tvir30	NOUN
cet-13508	85	41	enzyme	enzyme	NOUN
cet-13508	85	42	can	can	AUX
cet-13508	85	43	convert	convert	VERB
cet-13508	85	44	161	161	NUM
cet-13508	85	45	substrate	substrate	NOUN
cet-13508	85	46	molecules	molecule	NOUN
cet-13508	85	47	per	per	ADP
cet-13508	85	48	second	second	NOUN
cet-13508	85	49	at	at	ADP
cet-13508	85	50	saturation	saturation	NOUN
cet-13508	85	51	under	under	ADP
cet-13508	85	52	optimal	optimal	ADJ
cet-13508	85	53	conditions	condition	NOUN
cet-13508	85	54	.	.	PUNCT
cet-13508	86	1	finally	finally	ADV
cet-13508	86	2	,	,	PUNCT
cet-13508	86	3	the	the	DET
cet-13508	86	4	specificity	specificity	NOUN
cet-13508	86	5	constant	constant	ADJ
cet-13508	86	6	,	,	PUNCT
cet-13508	86	7	or	or	CCONJ
cet-13508	86	8	kcat	kcat	NOUN
cet-13508	86	9	/	/	SYM
cet-13508	86	10	km	km	NOUN
cet-13508	86	11	,	,	PUNCT
cet-13508	86	12	was	be	AUX
cet-13508	86	13	determined	determine	VERB
cet-13508	86	14	to	to	PART
cet-13508	86	15	be	be	AUX
cet-13508	86	16	85.85	85.85	NUM
cet-13508	86	17	ml	ml	NOUN
cet-13508	86	18	mg-1.s-1	mg-1.s-1	NOUN
cet-13508	86	19	.	.	PUNCT
cet-13508	87	1	the	the	DET
cet-13508	87	2	relatively	relatively	ADV
cet-13508	87	3	low	low	ADJ
cet-13508	87	4	km	km	NOUN
cet-13508	87	5	of	of	ADP
cet-13508	87	6	tvir30	tvir30	NOUN
cet-13508	87	7	indicates	indicate	VERB
cet-13508	87	8	that	that	SCONJ
cet-13508	87	9	the	the	DET
cet-13508	87	10	enzyme	enzyme	NOUN
cet-13508	87	11	has	have	VERB
cet-13508	87	12	high	high	ADJ
cet-13508	87	13	affinity	affinity	NOUN
cet-13508	87	14	for	for	ADP
cet-13508	87	15	its	its	PRON
cet-13508	87	16	substrate	substrate	NOUN
cet-13508	87	17	,	,	PUNCT
cet-13508	87	18	a	a	DET
cet-13508	87	19	common	common	ADJ
cet-13508	87	20	feature	feature	NOUN
cet-13508	87	21	of	of	ADP
cet-13508	87	22	enzymes	enzyme	NOUN
cet-13508	87	23	that	that	PRON
cet-13508	87	24	exist	exist	VERB
cet-13508	87	25	in	in	ADP
cet-13508	87	26	environments	environment	NOUN
cet-13508	87	27	with	with	ADP
cet-13508	87	28	a	a	DET
cet-13508	87	29	low	low	ADJ
cet-13508	87	30	abundance	abundance	NOUN
cet-13508	87	31	of	of	ADP
cet-13508	87	32	their	their	PRON
cet-13508	87	33	substrates	substrate	NOUN
cet-13508	87	34	(	(	PUNCT
cet-13508	87	35	nelson	nelson	PROPN
cet-13508	87	36	and	and	CCONJ
cet-13508	87	37	cox	cox	PROPN
cet-13508	87	38	,	,	PUNCT
cet-13508	87	39	2008	2008	NUM
cet-13508	87	40	)	)	PUNCT
cet-13508	87	41	.	.	PUNCT
cet-13508	88	1	in	in	ADP
cet-13508	88	2	the	the	DET
cet-13508	88	3	degradation	degradation	NOUN
cet-13508	88	4	of	of	ADP
cet-13508	88	5	bsy	bsy	NOUN
cet-13508	88	6	,	,	PUNCT
cet-13508	88	7	the	the	DET
cet-13508	88	8	high	high	ADJ
cet-13508	88	9	affinity	affinity	NOUN
cet-13508	88	10	of	of	ADP
cet-13508	88	11	tvir30	tvir30	NOUN
cet-13508	88	12	for	for	ADP
cet-13508	88	13	β-1,6	β-1,6	PROPN
cet-13508	88	14	-	-	PUNCT
cet-13508	88	15	glucan	glucan	NOUN
cet-13508	88	16	could	could	AUX
cet-13508	88	17	be	be	AUX
cet-13508	88	18	advantageous	advantageous	ADJ
cet-13508	88	19	as	as	SCONJ
cet-13508	88	20	it	it	PRON
cet-13508	88	21	accounts	account	VERB
cet-13508	88	22	for	for	ADP
cet-13508	88	23	only	only	ADV
cet-13508	88	24	10	10	NUM
cet-13508	88	25	-	-	SYM
cet-13508	88	26	15	15	NUM
cet-13508	88	27	%	%	NOUN
cet-13508	88	28	of	of	ADP
cet-13508	88	29	total	total	ADJ
cet-13508	88	30	polysaccharide	polysaccharide	NOUN
cet-13508	88	31	within	within	ADP
cet-13508	88	32	the	the	DET
cet-13508	88	33	s.	s.	PROPN
cet-13508	88	34	cerevisiae	cerevisiae	PROPN
cet-13508	88	35	cell	cell	NOUN
cet-13508	88	36	wall	wall	NOUN
cet-13508	88	37	,	,	PUNCT
cet-13508	88	38	and	and	CCONJ
cet-13508	88	39	is	be	AUX
cet-13508	88	40	in	in	ADP
cet-13508	88	41	complex	complex	ADJ
cet-13508	88	42	with	with	ADP
cet-13508	88	43	other	other	ADJ
cet-13508	88	44	cell	cell	NOUN
cet-13508	88	45	wall	wall	NOUN
cet-13508	88	46	components	component	NOUN
cet-13508	88	47	(	(	PUNCT
cet-13508	88	48	aimanianda	aimanianda	PROPN
cet-13508	88	49	et	et	PROPN
cet-13508	88	50	al	al	PROPN
cet-13508	88	51	.	.	PROPN
cet-13508	88	52	,	,	PUNCT
cet-13508	88	53	2009	2009	NUM
cet-13508	88	54	)	)	PUNCT
cet-13508	88	55	.	.	PUNCT
cet-13508	89	1	the	the	DET
cet-13508	89	2	kcat	kcat	PROPN
cet-13508	89	3	values	value	NOUN
cet-13508	89	4	of	of	ADP
cet-13508	89	5	other	other	ADJ
cet-13508	89	6	fungal	fungal	ADJ
cet-13508	89	7	glucanases	glucanase	NOUN
cet-13508	89	8	have	have	AUX
cet-13508	89	9	been	be	AUX
cet-13508	89	10	found	find	VERB
cet-13508	89	11	to	to	PART
cet-13508	89	12	be	be	AUX
cet-13508	89	13	as	as	ADV
cet-13508	89	14	low	low	ADJ
cet-13508	89	15	as	as	ADP
cet-13508	89	16	0.016	0.016	NUM
cet-13508	89	17	s-1	s-1	NOUN
cet-13508	89	18	and	and	CCONJ
cet-13508	89	19	as	as	ADV
cet-13508	89	20	high	high	ADJ
cet-13508	89	21	as	as	ADP
cet-13508	89	22	15,601	15,601	NUM
cet-13508	89	23	s-1	s-1	NOUN
cet-13508	89	24	(	(	PUNCT
cet-13508	89	25	boyce	boyce	NOUN
cet-13508	89	26	and	and	CCONJ
cet-13508	89	27	walsh	walsh	NOUN
cet-13508	89	28	,	,	PUNCT
cet-13508	89	29	2007	2007	NUM
cet-13508	89	30	;	;	PUNCT
cet-13508	90	1	yang	yang	PROPN
cet-13508	90	2	et	et	PROPN
cet-13508	90	3	al	al	PROPN
cet-13508	90	4	.	.	PROPN
cet-13508	90	5	,	,	PUNCT
cet-13508	90	6	2014	2014	NUM
cet-13508	90	7	)	)	PUNCT
cet-13508	90	8	.	.	PUNCT
cet-13508	91	1	the	the	DET
cet-13508	91	2	catalytic	catalytic	ADJ
cet-13508	91	3	efficiency	efficiency	NOUN
cet-13508	91	4	of	of	ADP
cet-13508	91	5	tvir30	tvir30	NOUN
cet-13508	91	6	is	be	AUX
cet-13508	91	7	therefore	therefore	ADV
cet-13508	91	8	within	within	ADP
cet-13508	91	9	the	the	DET
cet-13508	91	10	normal	normal	ADJ
cet-13508	91	11	range	range	NOUN
cet-13508	91	12	and	and	CCONJ
cet-13508	91	13	is	be	AUX
cet-13508	91	14	acceptable	acceptable	ADJ
cet-13508	91	15	for	for	ADP
cet-13508	91	16	industrial	industrial	ADJ
cet-13508	91	17	application	application	NOUN
cet-13508	91	18	.	.	PUNCT
cet-13508	92	1	3.5	3.5	NUM
cet-13508	92	2	products	product	NOUN
cet-13508	92	3	of	of	ADP
cet-13508	92	4	hydrolysis	hydrolysis	NOUN
cet-13508	92	5	tvir30	tvir30	NOUN
cet-13508	92	6	was	be	AUX
cet-13508	92	7	incubated	incubate	VERB
cet-13508	92	8	with	with	ADP
cet-13508	92	9	1	1	NUM
cet-13508	92	10	%	%	NOUN
cet-13508	92	11	(	(	PUNCT
cet-13508	92	12	w	w	NOUN
cet-13508	92	13	/	/	SYM
cet-13508	92	14	v	v	NOUN
cet-13508	92	15	)	)	PUNCT
cet-13508	92	16	pustulan	pustulan	NOUN
cet-13508	92	17	and	and	CCONJ
cet-13508	92	18	1	1	NUM
cet-13508	92	19	%	%	NOUN
cet-13508	92	20	(	(	PUNCT
cet-13508	92	21	w	w	NOUN
cet-13508	92	22	/	/	SYM
cet-13508	92	23	v	v	NOUN
cet-13508	92	24	)	)	PUNCT
cet-13508	92	25	alkali	alkali	ADJ
cet-13508	92	26	-	-	PUNCT
cet-13508	92	27	soluble	soluble	ADJ
cet-13508	92	28	yeast	yeast	NOUN
cet-13508	92	29	β	β	NOUN
cet-13508	92	30	-	-	PUNCT
cet-13508	92	31	glucan	glucan	NOUN
cet-13508	92	32	(	(	PUNCT
cet-13508	92	33	ybg	ybg	PROPN
cet-13508	92	34	)	)	PUNCT
cet-13508	92	35	for	for	ADP
cet-13508	92	36	1	1	NUM
cet-13508	92	37	hour	hour	NOUN
cet-13508	92	38	and	and	CCONJ
cet-13508	92	39	18	18	NUM
cet-13508	92	40	h	h	NOUN
cet-13508	92	41	respectively	respectively	ADV
cet-13508	92	42	at	at	ADP
cet-13508	92	43	40oc	40oc	PROPN
cet-13508	92	44	at	at	ADP
cet-13508	92	45	an	an	DET
cet-13508	92	46	appropriate	appropriate	ADJ
cet-13508	92	47	dilution	dilution	NOUN
cet-13508	92	48	.	.	PUNCT
cet-13508	93	1	the	the	DET
cet-13508	93	2	products	product	NOUN
cet-13508	93	3	of	of	ADP
cet-13508	93	4	hydrolysis	hydrolysis	NOUN
cet-13508	93	5	were	be	AUX
cet-13508	93	6	resolved	resolve	VERB
cet-13508	93	7	by	by	ADP
cet-13508	93	8	high	high	ADJ
cet-13508	93	9	hpaepad	hpaepad	NOUN
cet-13508	93	10	visualised	visualise	VERB
cet-13508	93	11	by	by	ADP
cet-13508	93	12	tlc	tlc	NOUN
cet-13508	93	13	as	as	SCONJ
cet-13508	93	14	described	describe	VERB
cet-13508	93	15	in	in	ADP
cet-13508	93	16	materials	material	NOUN
cet-13508	93	17	and	and	CCONJ
cet-13508	93	18	methods	method	NOUN
cet-13508	93	19	.	.	PUNCT
cet-13508	94	1	0.0	0.0	NUM
cet-13508	94	2	20.0	20.0	NUM
cet-13508	94	3	40.0	40.0	NUM
cet-13508	94	4	60.0	60.0	NUM
cet-13508	94	5	80.0	80.0	NUM
cet-13508	94	6	100.0	100.0	NUM
cet-13508	94	7	curdlan	curdlan	NOUN
cet-13508	94	8	cellulose	cellulose	NOUN
cet-13508	94	9	barley	barley	PROPN
cet-13508	94	10	βglucan	βglucan	PROPN
cet-13508	94	11	pustulan	pustulan	PROPN
cet-13508	95	1	lichenan	lichenan	PROPN
cet-13508	95	2	laminarin	laminarin	PROPN
cet-13508	95	3	m	m	VERB
cet-13508	95	4	e	e	NOUN
cet-13508	95	5	a	a	PRON
cet-13508	95	6	n	n	NOUN
cet-13508	95	7	r	r	NOUN
cet-13508	95	8	e	e	X
cet-13508	95	9	la	la	X
cet-13508	95	10	ti	ti	X
cet-13508	95	11	v	v	ADP
cet-13508	95	12	e	e	X
cet-13508	95	13	e	e	X
cet-13508	95	14	n	n	PROPN
cet-13508	95	15	z	z	PROPN
cet-13508	95	16	y	y	NOUN
cet-13508	95	17	m	m	VERB
cet-13508	95	18	e	e	NOUN
cet-13508	95	19	a	a	PRON
cet-13508	95	20	c	c	NOUN
cet-13508	95	21	ti	ti	NOUN
cet-13508	95	22	v	v	ADP
cet-13508	95	23	it	it	PRON
cet-13508	95	24	y	y	PROPN
cet-13508	95	25	(	(	PUNCT
cet-13508	95	26	%	%	NOUN
cet-13508	95	27	)	)	PUNCT
cet-13508	95	28	a.	a.	NOUN
cet-13508	95	29	0.0	0.0	NUM
cet-13508	95	30	20.0	20.0	NUM
cet-13508	95	31	40.0	40.0	NUM
cet-13508	95	32	60.0	60.0	NUM
cet-13508	95	33	80.0	80.0	NUM
cet-13508	95	34	100.0	100.0	NUM
cet-13508	95	35	120.0	120.0	NUM
cet-13508	95	36	0	0	NUM
cet-13508	95	37	0.25	0.25	NUM
cet-13508	95	38	0.5	0.5	NUM
cet-13508	95	39	0.75	0.75	NUM
cet-13508	95	40	1	1	NUM
cet-13508	95	41	m	m	NOUN
cet-13508	95	42	e	e	NOUN
cet-13508	95	43	a	a	DET
cet-13508	95	44	n	n	NOUN
cet-13508	95	45	r	r	NOUN
cet-13508	95	46	e	e	X
cet-13508	95	47	la	la	X
cet-13508	95	48	ti	ti	X
cet-13508	95	49	v	v	ADP
cet-13508	95	50	e	e	X
cet-13508	95	51	e	e	X
cet-13508	95	52	n	n	PROPN
cet-13508	95	53	z	z	PROPN
cet-13508	95	54	y	y	NOUN
cet-13508	95	55	m	m	VERB
cet-13508	95	56	e	e	NOUN
cet-13508	95	57	a	a	PRON
cet-13508	95	58	c	c	NOUN
cet-13508	95	59	ti	ti	NOUN
cet-13508	95	60	v	v	ADP
cet-13508	95	61	it	it	PRON
cet-13508	95	62	y	y	PROPN
cet-13508	95	63	(	(	PUNCT
cet-13508	95	64	%	%	NOUN
cet-13508	95	65	)	)	PUNCT
cet-13508	95	66	time	time	NOUN
cet-13508	95	67	(	(	PUNCT
cet-13508	95	68	h	h	NOUN
cet-13508	95	69	)	)	PUNCT
cet-13508	95	70	50	50	NUM
cet-13508	95	71	°	°	NUM
cet-13508	95	72	c	c	NOUN
cet-13508	95	73	0.0	0.0	NUM
cet-13508	95	74	20.0	20.0	NUM
cet-13508	95	75	40.0	40.0	NUM
cet-13508	95	76	60.0	60.0	NUM
cet-13508	95	77	80.0	80.0	NUM
cet-13508	95	78	100.0	100.0	NUM
cet-13508	95	79	120.0	120.0	NUM
cet-13508	95	80	0	0	NUM
cet-13508	95	81	20	20	NUM
cet-13508	95	82	40	40	NUM
cet-13508	95	83	60	60	NUM
cet-13508	95	84	80	80	NUM
cet-13508	95	85	100	100	NUM
cet-13508	95	86	time	time	NOUN
cet-13508	95	87	(	(	PUNCT
cet-13508	95	88	h	h	NOUN
cet-13508	95	89	)	)	PUNCT
cet-13508	95	90	40	40	NUM
cet-13508	95	91	°	°	NUM
cet-13508	95	92	cb	cb	PROPN
cet-13508	95	93	.	.	PUNCT
cet-13508	95	94	c.	c.	PROPN
cet-13508	95	95	106	106	NUM
cet-13508	95	96	hydrolysis	hydrolysis	NOUN
cet-13508	95	97	of	of	ADP
cet-13508	95	98	pustulan	pustulan	NOUN
cet-13508	95	99	determined	determine	VERB
cet-13508	95	100	that	that	DET
cet-13508	95	101	tvir30	tvir30	NOUN
cet-13508	95	102	has	have	VERB
cet-13508	95	103	an	an	DET
cet-13508	95	104	endolytic	endolytic	ADJ
cet-13508	95	105	mode	mode	NOUN
cet-13508	95	106	of	of	ADP
cet-13508	95	107	action	action	NOUN
cet-13508	95	108	,	,	PUNCT
cet-13508	95	109	as	as	SCONJ
cet-13508	95	110	it	it	PRON
cet-13508	95	111	can	can	AUX
cet-13508	95	112	liberate	liberate	VERB
cet-13508	95	113	a	a	DET
cet-13508	95	114	range	range	NOUN
cet-13508	95	115	of	of	ADP
cet-13508	95	116	sugars	sugar	NOUN
cet-13508	95	117	with	with	ADP
cet-13508	95	118	a	a	DET
cet-13508	95	119	dp	dp	NOUN
cet-13508	95	120	>	>	X
cet-13508	95	121	2	2	X
cet-13508	95	122	.	.	PUNCT
cet-13508	96	1	hpaec	hpaec	NOUN
cet-13508	96	2	analysis	analysis	NOUN
cet-13508	96	3	revealed	reveal	VERB
cet-13508	96	4	the	the	DET
cet-13508	96	5	main	main	ADJ
cet-13508	96	6	products	product	NOUN
cet-13508	96	7	of	of	ADP
cet-13508	96	8	hydrolysis	hydrolysis	NOUN
cet-13508	96	9	as	as	ADP
cet-13508	96	10	glucose	glucose	NOUN
cet-13508	96	11	(	(	PUNCT
cet-13508	96	12	peak	peak	NOUN
cet-13508	96	13	2	2	NUM
cet-13508	96	14	)	)	PUNCT
cet-13508	96	15	and	and	CCONJ
cet-13508	96	16	gentiobiose	gentiobiose	X
cet-13508	96	17	(	(	PUNCT
cet-13508	96	18	peak	peak	NOUN
cet-13508	96	19	3	3	NUM
cet-13508	96	20	)	)	PUNCT
cet-13508	96	21	(	(	PUNCT
cet-13508	96	22	figure	figure	NOUN
cet-13508	96	23	4a	4a	NUM
cet-13508	96	24	)	)	PUNCT
cet-13508	96	25	.	.	PUNCT
cet-13508	97	1	the	the	DET
cet-13508	97	2	peak	peak	NOUN
cet-13508	97	3	with	with	ADP
cet-13508	97	4	the	the	DET
cet-13508	97	5	largest	large	ADJ
cet-13508	97	6	area	area	NOUN
cet-13508	97	7	,	,	PUNCT
cet-13508	97	8	peak	peak	NOUN
cet-13508	97	9	1	1	NUM
cet-13508	97	10	,	,	PUNCT
cet-13508	97	11	likely	likely	ADV
cet-13508	97	12	arises	arise	VERB
cet-13508	97	13	from	from	ADP
cet-13508	97	14	the	the	DET
cet-13508	97	15	elution	elution	NOUN
cet-13508	97	16	of	of	ADP
cet-13508	97	17	glycerol	glycerol	NOUN
cet-13508	97	18	within	within	ADP
cet-13508	97	19	the	the	DET
cet-13508	97	20	enzyme	enzyme	NOUN
cet-13508	97	21	sample	sample	NOUN
cet-13508	97	22	.	.	PUNCT
cet-13508	98	1	besides	besides	SCONJ
cet-13508	98	2	glucose	glucose	NOUN
cet-13508	98	3	and	and	CCONJ
cet-13508	98	4	gentiobiose	gentiobiose	NOUN
cet-13508	98	5	,	,	PUNCT
cet-13508	98	6	five	five	NUM
cet-13508	98	7	additional	additional	ADJ
cet-13508	98	8	distinct	distinct	ADJ
cet-13508	98	9	peaks	peak	NOUN
cet-13508	98	10	are	be	AUX
cet-13508	98	11	visible	visible	ADJ
cet-13508	98	12	,	,	PUNCT
cet-13508	98	13	and	and	CCONJ
cet-13508	98	14	the	the	DET
cet-13508	98	15	tlc	tlc	NOUN
cet-13508	98	16	plate	plate	NOUN
cet-13508	98	17	in	in	ADP
cet-13508	98	18	figure	figure	NOUN
cet-13508	98	19	4b	4b	PROPN
cet-13508	98	20	indicates	indicate	VERB
cet-13508	98	21	that	that	SCONJ
cet-13508	98	22	these	these	PRON
cet-13508	98	23	may	may	AUX
cet-13508	98	24	be	be	AUX
cet-13508	98	25	gentiotriose	gentiotriose	VERB
cet-13508	98	26	,	,	PUNCT
cet-13508	98	27	gentiotetraose	gentiotetraose	NOUN
cet-13508	98	28	,	,	PUNCT
cet-13508	98	29	gentiopentaose	gentiopentaose	NOUN
cet-13508	98	30	,	,	PUNCT
cet-13508	98	31	and	and	CCONJ
cet-13508	98	32	so	so	ADV
cet-13508	98	33	on	on	ADV
cet-13508	98	34	.	.	PUNCT
cet-13508	99	1	figure	figure	VERB
cet-13508	99	2	4	4	NUM
cet-13508	99	3	:	:	PUNCT
cet-13508	99	4	a.	a.	NOUN
cet-13508	99	5	hpae	hpae	NOUN
cet-13508	99	6	-	-	PUNCT
cet-13508	99	7	pad	pad	NOUN
cet-13508	99	8	analysis	analysis	NOUN
cet-13508	99	9	and	and	CCONJ
cet-13508	99	10	b.	b.	PROPN
cet-13508	99	11	tlc	tlc	NOUN
cet-13508	99	12	analysis	analysis	NOUN
cet-13508	99	13	of	of	ADP
cet-13508	99	14	a	a	DET
cet-13508	99	15	sample	sample	NOUN
cet-13508	99	16	of	of	ADP
cet-13508	99	17	pustulan	pustulan	NOUN
cet-13508	99	18	hydrolysed	hydrolyse	VERB
cet-13508	99	19	by	by	ADP
cet-13508	99	20	tvir30	tvir30	NOUN
cet-13508	99	21	for	for	ADP
cet-13508	99	22	1	1	NUM
cet-13508	99	23	h	h	NOUN
cet-13508	99	24	at	at	ADP
cet-13508	99	25	40oc	40oc	ADJ
cet-13508	99	26	.	.	PUNCT
cet-13508	100	1	to	to	PART
cet-13508	100	2	determine	determine	VERB
cet-13508	100	3	if	if	SCONJ
cet-13508	100	4	tvir30	tvir30	NOUN
cet-13508	100	5	could	could	AUX
cet-13508	100	6	be	be	AUX
cet-13508	100	7	used	use	VERB
cet-13508	100	8	to	to	PART
cet-13508	100	9	generate	generate	VERB
cet-13508	100	10	gnos	gno	NOUN
cet-13508	100	11	from	from	ADP
cet-13508	100	12	brewer	brewer	NOUN
cet-13508	100	13	’s	’s	PART
cet-13508	100	14	spent	spend	VERB
cet-13508	100	15	yeast	yeast	NOUN
cet-13508	100	16	,	,	PUNCT
cet-13508	100	17	the	the	DET
cet-13508	100	18	enzyme	enzyme	NOUN
cet-13508	100	19	was	be	AUX
cet-13508	100	20	incubated	incubate	VERB
cet-13508	100	21	with	with	ADP
cet-13508	100	22	1	1	NUM
cet-13508	100	23	%	%	NOUN
cet-13508	100	24	(	(	PUNCT
cet-13508	100	25	w	w	NOUN
cet-13508	100	26	/	/	SYM
cet-13508	100	27	v	v	NOUN
cet-13508	100	28	)	)	PUNCT
cet-13508	100	29	alkali	alkali	ADJ
cet-13508	100	30	-	-	PUNCT
cet-13508	100	31	soluble	soluble	ADJ
cet-13508	100	32	yeast	yeast	NOUN
cet-13508	100	33	β	β	NOUN
cet-13508	100	34	-	-	NOUN
cet-13508	100	35	glucan	glucan	NOUN
cet-13508	100	36	.	.	PUNCT
cet-13508	101	1	the	the	DET
cet-13508	101	2	reaction	reaction	NOUN
cet-13508	101	3	was	be	AUX
cet-13508	101	4	analysed	analyse	VERB
cet-13508	101	5	by	by	ADP
cet-13508	101	6	hpaec	hpaec	NOUN
cet-13508	101	7	and	and	CCONJ
cet-13508	101	8	tlc	tlc	NOUN
cet-13508	101	9	as	as	SCONJ
cet-13508	101	10	shown	show	VERB
cet-13508	101	11	in	in	ADP
cet-13508	101	12	figure	figure	NOUN
cet-13508	101	13	5	5	NUM
cet-13508	101	14	.	.	PUNCT
cet-13508	102	1	peak	peak	NOUN
cet-13508	102	2	1	1	NUM
cet-13508	102	3	in	in	ADP
cet-13508	102	4	figure	figure	NOUN
cet-13508	102	5	5a	5a	NUM
cet-13508	102	6	is	be	AUX
cet-13508	102	7	presumed	presume	VERB
cet-13508	102	8	to	to	PART
cet-13508	102	9	be	be	AUX
cet-13508	102	10	glycerol	glycerol	NOUN
cet-13508	102	11	within	within	ADP
cet-13508	102	12	the	the	DET
cet-13508	102	13	sample	sample	NOUN
cet-13508	102	14	eluting	elute	VERB
cet-13508	102	15	from	from	ADP
cet-13508	102	16	the	the	DET
cet-13508	102	17	column	column	NOUN
cet-13508	102	18	.	.	PUNCT
cet-13508	103	1	peaks	peak	NOUN
cet-13508	103	2	2	2	NUM
cet-13508	103	3	and	and	CCONJ
cet-13508	103	4	3	3	NUM
cet-13508	103	5	are	be	AUX
cet-13508	103	6	glucose	glucose	NOUN
cet-13508	103	7	and	and	CCONJ
cet-13508	103	8	gentiobiose	gentiobiose	VERB
cet-13508	103	9	respectively	respectively	ADV
cet-13508	103	10	.	.	PUNCT
cet-13508	104	1	there	there	PRON
cet-13508	104	2	is	be	VERB
cet-13508	104	3	one	one	NUM
cet-13508	104	4	more	more	ADV
cet-13508	104	5	distinct	distinct	ADJ
cet-13508	104	6	peak	peak	NOUN
cet-13508	104	7	,	,	PUNCT
cet-13508	104	8	labelled	label	VERB
cet-13508	104	9	peak	peak	NOUN
cet-13508	104	10	5	5	NUM
cet-13508	104	11	,	,	PUNCT
cet-13508	104	12	as	as	ADV
cet-13508	104	13	well	well	ADV
cet-13508	104	14	as	as	ADP
cet-13508	104	15	additional	additional	ADJ
cet-13508	104	16	smaller	small	ADJ
cet-13508	104	17	peaks	peak	NOUN
cet-13508	104	18	.	.	PUNCT
cet-13508	105	1	these	these	PRON
cet-13508	105	2	are	be	AUX
cet-13508	105	3	presumed	presume	VERB
cet-13508	105	4	to	to	PART
cet-13508	105	5	be	be	AUX
cet-13508	105	6	larger	large	ADJ
cet-13508	105	7	oligosaccharides	oligosaccharide	NOUN
cet-13508	105	8	generated	generate	VERB
cet-13508	105	9	by	by	ADP
cet-13508	105	10	tvir30	tvir30	ADJ
cet-13508	105	11	digestion	digestion	NOUN
cet-13508	105	12	as	as	SCONJ
cet-13508	105	13	tlc	tlc	NOUN
cet-13508	105	14	analysis	analysis	NOUN
cet-13508	105	15	indicated	indicate	VERB
cet-13508	105	16	the	the	DET
cet-13508	105	17	presence	presence	NOUN
cet-13508	105	18	of	of	ADP
cet-13508	105	19	sugars	sugar	NOUN
cet-13508	105	20	larger	large	ADJ
cet-13508	105	21	than	than	ADP
cet-13508	105	22	gentiobiose	gentiobiose	NOUN
cet-13508	105	23	(	(	PUNCT
cet-13508	105	24	in	in	ADP
cet-13508	105	25	the	the	DET
cet-13508	105	26	boxed	boxed	ADJ
cet-13508	105	27	region	region	NOUN
cet-13508	105	28	of	of	ADP
cet-13508	105	29	figure	figure	NOUN
cet-13508	105	30	5b	5b	NUM
cet-13508	105	31	)	)	PUNCT
cet-13508	105	32	.	.	PUNCT
cet-13508	106	1	the	the	DET
cet-13508	106	2	poor	poor	ADJ
cet-13508	106	3	resolution	resolution	NOUN
cet-13508	106	4	of	of	ADP
cet-13508	106	5	oligosaccharides	oligosaccharide	NOUN
cet-13508	106	6	dp>2	dp>2	PROPN
cet-13508	106	7	on	on	ADP
cet-13508	106	8	the	the	DET
cet-13508	106	9	tlc	tlc	NOUN
cet-13508	106	10	plate	plate	NOUN
cet-13508	106	11	is	be	AUX
cet-13508	106	12	likely	likely	ADJ
cet-13508	106	13	the	the	DET
cet-13508	106	14	result	result	NOUN
cet-13508	106	15	of	of	ADP
cet-13508	106	16	their	their	PRON
cet-13508	106	17	low	low	ADJ
cet-13508	106	18	concentration	concentration	NOUN
cet-13508	106	19	.	.	PUNCT
cet-13508	107	1	these	these	DET
cet-13508	107	2	results	result	NOUN
cet-13508	107	3	demonstrate	demonstrate	VERB
cet-13508	107	4	that	that	DET
cet-13508	107	5	tvir30	tvir30	NOUN
cet-13508	107	6	may	may	AUX
cet-13508	107	7	be	be	AUX
cet-13508	107	8	employed	employ	VERB
cet-13508	107	9	in	in	ADP
cet-13508	107	10	the	the	DET
cet-13508	107	11	utilisation	utilisation	NOUN
cet-13508	107	12	of	of	ADP
cet-13508	107	13	bsy	bsy	NOUN
cet-13508	107	14	by	by	ADP
cet-13508	107	15	generating	generate	VERB
cet-13508	107	16	value	value	NOUN
cet-13508	107	17	-	-	PUNCT
cet-13508	107	18	added	add	VERB
cet-13508	107	19	products	product	NOUN
cet-13508	107	20	in	in	ADP
cet-13508	107	21	the	the	DET
cet-13508	107	22	form	form	NOUN
cet-13508	107	23	of	of	ADP
cet-13508	107	24	prebiotic	prebiotic	ADJ
cet-13508	107	25	gnos	gno	NOUN
cet-13508	107	26	.	.	PUNCT
cet-13508	108	1	4	4	X
cet-13508	108	2	.	.	X
cet-13508	108	3	conclusions	conclusion	NOUN
cet-13508	108	4	in	in	ADP
cet-13508	108	5	conclusion	conclusion	NOUN
cet-13508	108	6	,	,	PUNCT
cet-13508	108	7	a	a	DET
cet-13508	108	8	fungal	fungal	ADJ
cet-13508	108	9	β-1,6	β-1,6	NOUN
cet-13508	108	10	-	-	NOUN
cet-13508	108	11	glucanase	glucanase	NOUN
cet-13508	108	12	from	from	ADP
cet-13508	108	13	the	the	DET
cet-13508	108	14	glycoside	glycoside	NOUN
cet-13508	108	15	hydrolase	hydrolase	NOUN
cet-13508	108	16	30	30	NUM
cet-13508	108	17	family	family	NOUN
cet-13508	108	18	was	be	AUX
cet-13508	108	19	successfully	successfully	ADV
cet-13508	108	20	cloned	clone	VERB
cet-13508	108	21	,	,	PUNCT
cet-13508	108	22	expressed	express	VERB
cet-13508	108	23	,	,	PUNCT
cet-13508	108	24	and	and	CCONJ
cet-13508	108	25	purified	purified	ADJ
cet-13508	108	26	.	.	PUNCT
cet-13508	109	1	the	the	DET
cet-13508	109	2	enzyme	enzyme	NOUN
cet-13508	109	3	,	,	PUNCT
cet-13508	109	4	tvir30	tvir30	NOUN
cet-13508	109	5	,	,	PUNCT
cet-13508	109	6	exhibited	exhibit	VERB
cet-13508	109	7	predicted	predict	VERB
cet-13508	109	8	substrate	substrate	NOUN
cet-13508	109	9	specificity	specificity	NOUN
cet-13508	109	10	to	to	ADP
cet-13508	109	11	β-1,6	β-1,6	NOUN
cet-13508	109	12	-	-	NOUN
cet-13508	109	13	linkages	linkage	NOUN
cet-13508	109	14	only	only	ADV
cet-13508	109	15	and	and	CCONJ
cet-13508	109	16	had	have	VERB
cet-13508	109	17	kinetic	kinetic	ADJ
cet-13508	109	18	properties	property	NOUN
cet-13508	109	19	similar	similar	ADJ
cet-13508	109	20	to	to	ADP
cet-13508	109	21	those	those	PRON
cet-13508	109	22	of	of	ADP
cet-13508	109	23	other	other	ADJ
cet-13508	109	24	purified	purified	ADJ
cet-13508	109	25	fungal	fungal	ADJ
cet-13508	109	26	β-1,6	β-1,6	NOUN
cet-13508	109	27	-	-	PUNCT
cet-13508	109	28	glucanases	glucanase	NOUN
cet-13508	109	29	.	.	PUNCT
cet-13508	110	1	a	a	DET
cet-13508	110	2	unique	unique	ADJ
cet-13508	110	3	feature	feature	NOUN
cet-13508	110	4	of	of	ADP
cet-13508	110	5	tvir30	tvir30	NOUN
cet-13508	110	6	is	be	AUX
cet-13508	110	7	its	its	PRON
cet-13508	110	8	expression	expression	NOUN
cet-13508	110	9	as	as	ADP
cet-13508	110	10	two	two	NUM
cet-13508	110	11	isoforms	isoform	NOUN
cet-13508	110	12	,	,	PUNCT
cet-13508	110	13	one	one	NUM
cet-13508	110	14	of	of	ADP
cet-13508	110	15	which	which	PRON
cet-13508	110	16	appears	appear	VERB
cet-13508	110	17	glycosylated	glycosylate	VERB
cet-13508	110	18	.	.	PUNCT
cet-13508	111	1	whether	whether	SCONJ
cet-13508	111	2	native	native	ADJ
cet-13508	111	3	expression	expression	NOUN
cet-13508	111	4	in	in	ADP
cet-13508	111	5	trichoderma	trichoderma	NOUN
cet-13508	111	6	virens	viren	NOUN
cet-13508	111	7	would	would	AUX
cet-13508	111	8	produce	produce	VERB
cet-13508	111	9	a	a	DET
cet-13508	111	10	glycosylated	glycosylated	ADJ
cet-13508	111	11	variant	variant	NOUN
cet-13508	111	12	is	be	AUX
cet-13508	111	13	unclear	unclear	ADJ
cet-13508	111	14	,	,	PUNCT
cet-13508	111	15	but	but	CCONJ
cet-13508	111	16	the	the	DET
cet-13508	111	17	amino	amino	NOUN
cet-13508	111	18	acid	acid	NOUN
cet-13508	111	19	sequence	sequence	NOUN
cet-13508	111	20	does	do	AUX
cet-13508	111	21	encode	encode	VERB
cet-13508	111	22	for	for	ADP
cet-13508	111	23	two	two	NUM
cet-13508	111	24	n1	n1	NOUN
cet-13508	111	25	2	2	NUM
cet-13508	111	26	3	3	NUM
cet-13508	111	27	4	4	NUM
cet-13508	111	28	1	1	NUM
cet-13508	111	29	.	.	PUNCT
cet-13508	112	1	glucose	glucose	NOUN
cet-13508	112	2	2	2	NUM
cet-13508	112	3	.	.	PUNCT
cet-13508	112	4	gentiobiose	gentiobiose	VERB
cet-13508	112	5	3	3	NUM
cet-13508	112	6	.	.	PUNCT
cet-13508	112	7	pustulan	pustulan	NOUN
cet-13508	112	8	hydrolysis	hydrolysis	NOUN
cet-13508	112	9	,	,	PUNCT
cet-13508	112	10	1	1	NUM
cet-13508	112	11	h	h	NOUN
cet-13508	112	12	4	4	NUM
cet-13508	112	13	.	.	PUNCT
cet-13508	112	14	pustulan	pustulan	NOUN
cet-13508	112	15	hydrolysis	hydrolysis	NOUN
cet-13508	112	16	blank	blank	PROPN
cet-13508	112	17	b.	b.	PROPN
cet-13508	112	18	a.	a.	PROPN
cet-13508	112	19	a.	a.	PROPN
cet-13508	112	20	b.	b.	PROPN
cet-13508	113	1	1	1	X
cet-13508	113	2	.	.	X
cet-13508	113	3	ybg	ybg	PROPN
cet-13508	113	4	spiked	spike	VERB
cet-13508	113	5	with	with	ADP
cet-13508	113	6	glucose	glucose	NOUN
cet-13508	113	7	2	2	NUM
cet-13508	113	8	.	.	PUNCT
cet-13508	113	9	ybg	ybg	PROPN
cet-13508	113	10	spiked	spike	VERB
cet-13508	113	11	with	with	ADP
cet-13508	113	12	gentiobiose	gentiobiose	NOUN
cet-13508	113	13	3	3	NUM
cet-13508	113	14	.	.	PUNCT
cet-13508	113	15	ybg	ybg	NOUN
cet-13508	113	16	hydrolysis	hydrolysis	NOUN
cet-13508	113	17	,	,	PUNCT
cet-13508	113	18	18	18	NUM
cet-13508	113	19	h	h	NOUN
cet-13508	113	20	4	4	NUM
cet-13508	113	21	.	.	PUNCT
cet-13508	114	1	ybg	ybg	NOUN
cet-13508	114	2	hydrolysis	hydrolysis	NOUN
cet-13508	114	3	blank	blank	ADJ
cet-13508	114	4	1	1	NUM
cet-13508	114	5	2	2	NUM
cet-13508	114	6	3	3	NUM
cet-13508	114	7	4	4	NUM
cet-13508	114	8	figure	figure	NOUN
cet-13508	114	9	5	5	NUM
cet-13508	114	10	:	:	PUNCT
cet-13508	114	11	a.	a.	NOUN
cet-13508	114	12	hpae	hpae	NOUN
cet-13508	114	13	-	-	PUNCT
cet-13508	114	14	pad	pad	NOUN
cet-13508	114	15	analysis	analysis	NOUN
cet-13508	114	16	and	and	CCONJ
cet-13508	114	17	b.	b.	PROPN
cet-13508	114	18	tlc	tlc	NOUN
cet-13508	114	19	analysis	analysis	NOUN
cet-13508	114	20	of	of	ADP
cet-13508	114	21	a	a	DET
cet-13508	114	22	sample	sample	NOUN
cet-13508	114	23	of	of	ADP
cet-13508	114	24	yeast	yeast	NOUN
cet-13508	114	25	β	β	NOUN
cet-13508	114	26	-	-	PUNCT
cet-13508	114	27	glucan	glucan	NOUN
cet-13508	114	28	hydrolysed	hydrolyse	VERB
cet-13508	114	29	by	by	ADP
cet-13508	114	30	tvir30	tvir30	NOUN
cet-13508	114	31	for	for	ADP
cet-13508	114	32	18	18	NUM
cet-13508	114	33	h	h	NOUN
cet-13508	114	34	at	at	ADP
cet-13508	114	35	40oc	40oc	ADJ
cet-13508	114	36	.	.	PUNCT
cet-13508	115	1	107	107	NUM
cet-13508	115	2	linked	link	VERB
cet-13508	115	3	glycosylation	glycosylation	NOUN
cet-13508	115	4	sites	site	NOUN
cet-13508	115	5	,	,	PUNCT
cet-13508	115	6	and	and	CCONJ
cet-13508	115	7	fungal	fungal	ADJ
cet-13508	115	8	mycoparasites	mycoparasite	NOUN
cet-13508	115	9	are	be	AUX
cet-13508	115	10	known	know	VERB
cet-13508	115	11	to	to	PART
cet-13508	115	12	produce	produce	VERB
cet-13508	115	13	a	a	DET
cet-13508	115	14	diverse	diverse	ADJ
cet-13508	115	15	range	range	NOUN
cet-13508	115	16	of	of	ADP
cet-13508	115	17	hydrolytic	hydrolytic	ADJ
cet-13508	115	18	enzymes	enzyme	NOUN
cet-13508	115	19	,	,	PUNCT
cet-13508	115	20	often	often	ADV
cet-13508	115	21	in	in	ADP
cet-13508	115	22	one	one	NUM
cet-13508	115	23	glycoside	glycoside	NOUN
cet-13508	115	24	hydrolase	hydrolase	NOUN
cet-13508	115	25	family	family	NOUN
cet-13508	115	26	,	,	PUNCT
cet-13508	115	27	to	to	PART
cet-13508	115	28	efficiently	efficiently	ADV
cet-13508	115	29	break	break	VERB
cet-13508	115	30	down	down	ADP
cet-13508	115	31	the	the	DET
cet-13508	115	32	cell	cell	NOUN
cet-13508	115	33	walls	wall	NOUN
cet-13508	115	34	of	of	ADP
cet-13508	115	35	their	their	PRON
cet-13508	115	36	hosts	host	NOUN
cet-13508	115	37	.	.	PUNCT
cet-13508	116	1	for	for	ADP
cet-13508	116	2	an	an	DET
cet-13508	116	3	industrial	industrial	ADJ
cet-13508	116	4	application	application	NOUN
cet-13508	116	5	,	,	PUNCT
cet-13508	116	6	resolution	resolution	NOUN
cet-13508	116	7	of	of	ADP
cet-13508	116	8	isoforms	isoform	NOUN
cet-13508	116	9	may	may	AUX
cet-13508	116	10	be	be	AUX
cet-13508	116	11	unnecessary	unnecessary	ADJ
cet-13508	116	12	if	if	SCONJ
cet-13508	116	13	batch	batch	NOUN
cet-13508	116	14	-	-	PUNCT
cet-13508	116	15	to	to	ADP
cet-13508	116	16	-	-	PUNCT
cet-13508	116	17	batch	batch	NOUN
cet-13508	116	18	variability	variability	NOUN
cet-13508	116	19	is	be	AUX
cet-13508	116	20	negligible	negligible	ADJ
cet-13508	116	21	.	.	PUNCT
cet-13508	117	1	tvir30	tvir30	PROPN
cet-13508	117	2	demonstrates	demonstrate	VERB
cet-13508	117	3	both	both	DET
cet-13508	117	4	high	high	ADJ
cet-13508	117	5	affinity	affinity	NOUN
cet-13508	117	6	and	and	CCONJ
cet-13508	117	7	activity	activity	NOUN
cet-13508	117	8	against	against	ADP
cet-13508	117	9	β-1,6	β-1,6	NOUN
cet-13508	117	10	-	-	NOUN
cet-13508	117	11	linkages	linkage	NOUN
cet-13508	117	12	,	,	PUNCT
cet-13508	117	13	as	as	ADV
cet-13508	117	14	well	well	ADV
cet-13508	117	15	as	as	ADP
cet-13508	117	16	an	an	DET
cet-13508	117	17	endolytic	endolytic	ADJ
cet-13508	117	18	mode	mode	NOUN
cet-13508	117	19	of	of	ADP
cet-13508	117	20	action	action	NOUN
cet-13508	117	21	,	,	PUNCT
cet-13508	117	22	making	make	VERB
cet-13508	117	23	it	it	PRON
cet-13508	117	24	a	a	DET
cet-13508	117	25	favourable	favourable	ADJ
cet-13508	117	26	candidate	candidate	NOUN
cet-13508	117	27	in	in	ADP
cet-13508	117	28	the	the	DET
cet-13508	117	29	degradation	degradation	NOUN
cet-13508	117	30	of	of	ADP
cet-13508	117	31	bsy	bsy	NOUN
cet-13508	117	32	for	for	ADP
cet-13508	117	33	the	the	DET
cet-13508	117	34	production	production	NOUN
cet-13508	117	35	of	of	ADP
cet-13508	117	36	gnos	gno	NOUN
cet-13508	117	37	.	.	PUNCT
cet-13508	118	1	the	the	DET
cet-13508	118	2	enzyme	enzyme	NOUN
cet-13508	118	3	is	be	AUX
cet-13508	118	4	thermally	thermally	ADV
cet-13508	118	5	inactivated	inactivate	VERB
cet-13508	118	6	by	by	ADP
cet-13508	118	7	only	only	ADV
cet-13508	118	8	30	30	NUM
cet-13508	118	9	min	min	NOUN
cet-13508	118	10	incubation	incubation	NOUN
cet-13508	118	11	at	at	ADP
cet-13508	118	12	50oc	50oc	PROPN
cet-13508	118	13	but	but	CCONJ
cet-13508	118	14	was	be	AUX
cet-13508	118	15	found	find	VERB
cet-13508	118	16	to	to	PART
cet-13508	118	17	be	be	AUX
cet-13508	118	18	highly	highly	ADV
cet-13508	118	19	stable	stable	ADJ
cet-13508	118	20	at	at	ADP
cet-13508	118	21	40oc	40oc	PROPN
cet-13508	118	22	.	.	PUNCT
cet-13508	119	1	from	from	ADP
cet-13508	119	2	the	the	DET
cet-13508	119	3	characterisation	characterisation	NOUN
cet-13508	119	4	performed	perform	VERB
cet-13508	119	5	in	in	ADP
cet-13508	119	6	this	this	DET
cet-13508	119	7	study	study	NOUN
cet-13508	119	8	,	,	PUNCT
cet-13508	119	9	a	a	DET
cet-13508	119	10	novel	novel	NOUN
cet-13508	119	11	and	and	CCONJ
cet-13508	119	12	efficient	efficient	ADJ
cet-13508	119	13	method	method	NOUN
cet-13508	119	14	for	for	ADP
cet-13508	119	15	the	the	DET
cet-13508	119	16	enzymatic	enzymatic	ADJ
cet-13508	119	17	degradation	degradation	NOUN
cet-13508	119	18	of	of	ADP
cet-13508	119	19	bsy	bsy	NOUN
cet-13508	119	20	could	could	AUX
cet-13508	119	21	be	be	AUX
cet-13508	119	22	developed	develop	VERB
cet-13508	119	23	to	to	PART
cet-13508	119	24	generate	generate	VERB
cet-13508	119	25	value	value	NOUN
cet-13508	119	26	-	-	PUNCT
cet-13508	119	27	added	add	VERB
cet-13508	119	28	oligosaccharides	oligosaccharide	NOUN
cet-13508	119	29	,	,	PUNCT
cet-13508	119	30	while	while	SCONJ
cet-13508	119	31	reducing	reduce	VERB
cet-13508	119	32	waste	waste	NOUN
cet-13508	119	33	and	and	CCONJ
cet-13508	119	34	waste	waste	NOUN
cet-13508	119	35	-	-	PUNCT
cet-13508	119	36	disposal	disposal	NOUN
cet-13508	119	37	costs	cost	NOUN
cet-13508	119	38	.	.	PUNCT
cet-13508	120	1	acknowledgments	acknowledgment	NOUN
cet-13508	120	2	this	this	DET
cet-13508	120	3	study	study	NOUN
cet-13508	120	4	was	be	AUX
cet-13508	120	5	funded	fund	VERB
cet-13508	120	6	by	by	ADP
cet-13508	120	7	the	the	DET
cet-13508	120	8	irish	irish	PROPN
cet-13508	120	9	research	research	PROPN
cet-13508	120	10	council	council	PROPN
cet-13508	120	11	and	and	CCONJ
cet-13508	120	12	monaghan	monaghan	PROPN
cet-13508	120	13	mushrooms	mushroom	NOUN
cet-13508	120	14	.	.	PUNCT
cet-13508	121	1	references	reference	NOUN
cet-13508	121	2	aimanianda	aimanianda	ADV
cet-13508	121	3	,	,	PUNCT
cet-13508	121	4	v.	v.	ADV
cet-13508	121	5	,	,	PUNCT
cet-13508	121	6	clavaud	clavaud	PROPN
cet-13508	121	7	,	,	PUNCT
cet-13508	121	8	c.	c.	NOUN
cet-13508	121	9	,	,	PUNCT
cet-13508	121	10	simenel	simenel	NOUN
cet-13508	121	11	,	,	PUNCT
cet-13508	121	12	c.	c.	PROPN
cet-13508	121	13	,	,	PUNCT
cet-13508	121	14	fontaine	fontaine	PROPN
cet-13508	121	15	,	,	PUNCT
cet-13508	121	16	t.	t.	PROPN
cet-13508	121	17	,	,	PUNCT
cet-13508	121	18	delepierre	delepierre	NOUN
cet-13508	121	19	,	,	PUNCT
cet-13508	121	20	m.	m.	NOUN
cet-13508	121	21	,	,	PUNCT
cet-13508	121	22	latgé	latgé	NOUN
cet-13508	121	23	,	,	PUNCT
cet-13508	121	24	j.-p	j.-p	PROPN
cet-13508	121	25	.	.	PROPN
cet-13508	121	26	,	,	PUNCT
cet-13508	121	27	2009	2009	NUM
cet-13508	121	28	.	.	PUNCT
cet-13508	122	1	cell	cell	NOUN
cet-13508	122	2	wall	wall	NOUN
cet-13508	122	3	beta-(1,6)glucan	beta-(1,6)glucan	PROPN
cet-13508	122	4	of	of	ADP
cet-13508	122	5	saccharomyces	saccharomyce	NOUN
cet-13508	122	6	cerevisiae	cerevisiae	NOUN
cet-13508	122	7	:	:	PUNCT
cet-13508	122	8	structural	structural	ADJ
cet-13508	122	9	characterization	characterization	NOUN
cet-13508	122	10	and	and	CCONJ
cet-13508	122	11	in	in	ADP
cet-13508	122	12	situ	situ	ADJ
cet-13508	122	13	synthesis	synthesis	NOUN
cet-13508	122	14	.	.	PUNCT
cet-13508	123	1	j	j	PROPN
cet-13508	123	2	biol	biol	PROPN
cet-13508	123	3	chem	chem	NOUN
cet-13508	123	4	284	284	NUM
cet-13508	123	5	,	,	PUNCT
cet-13508	123	6	13401–13412	13401–13412	NUM
cet-13508	123	7	.	.	PUNCT
cet-13508	124	1	https://doi.org/10.1074/jbc.m807667200	https://doi.org/10.1074/jbc.m807667200	PROPN
cet-13508	124	2	boyce	boyce	PROPN
cet-13508	124	3	,	,	PUNCT
cet-13508	124	4	a.	a.	PROPN
cet-13508	124	5	,	,	PUNCT
cet-13508	124	6	walsh	walsh	PROPN
cet-13508	124	7	,	,	PUNCT
cet-13508	124	8	g.	g.	PROPN
cet-13508	124	9	,	,	PUNCT
cet-13508	124	10	2007	2007	NUM
cet-13508	124	11	.	.	PUNCT
cet-13508	125	1	production	production	NOUN
cet-13508	125	2	,	,	PUNCT
cet-13508	125	3	purification	purification	NOUN
cet-13508	125	4	and	and	CCONJ
cet-13508	125	5	application	application	NOUN
cet-13508	125	6	-	-	PUNCT
cet-13508	125	7	relevant	relevant	ADJ
cet-13508	125	8	characterisation	characterisation	NOUN
cet-13508	125	9	of	of	ADP
cet-13508	125	10	an	an	DET
cet-13508	125	11	endo-1,3(4)beta	endo-1,3(4)beta	NOUN
cet-13508	125	12	-	-	PUNCT
cet-13508	125	13	glucanase	glucanase	NOUN
cet-13508	125	14	from	from	ADP
cet-13508	125	15	rhizomucor	rhizomucor	PROPN
cet-13508	125	16	miehei	miehei	PROPN
cet-13508	125	17	.	.	PUNCT
cet-13508	126	1	appl	appl	PROPN
cet-13508	126	2	microbiol	microbiol	PROPN
cet-13508	126	3	biotechnol	biotechnol	PROPN
cet-13508	126	4	76	76	NUM
cet-13508	126	5	,	,	PUNCT
cet-13508	126	6	835–841	835–841	NUM
cet-13508	126	7	.	.	PUNCT
cet-13508	127	1	https://doi.org/10.1007/s00253-007-1058-x	https://doi.org/10.1007/s00253-007-1058-x	PROPN
cet-13508	127	2	cruz	cruz	PROPN
cet-13508	127	3	,	,	PUNCT
cet-13508	127	4	j.	j.	PROPN
cet-13508	127	5	de	de	PROPN
cet-13508	127	6	la	la	PROPN
cet-13508	127	7	,	,	PUNCT
cet-13508	127	8	llobell	llobell	PROPN
cet-13508	127	9	,	,	PUNCT
cet-13508	127	10	a.	a.	NOUN
cet-13508	127	11	,	,	PUNCT
cet-13508	127	12	1999	1999	NUM
cet-13508	127	13	.	.	PUNCT
cet-13508	128	1	purification	purification	NOUN
cet-13508	128	2	and	and	CCONJ
cet-13508	128	3	properties	property	NOUN
cet-13508	128	4	of	of	ADP
cet-13508	128	5	a	a	DET
cet-13508	128	6	basic	basic	ADJ
cet-13508	128	7	endo	endo	NOUN
cet-13508	128	8	-	-	PUNCT
cet-13508	128	9	β-1,6	β-1,6	NOUN
cet-13508	128	10	-	-	ADJ
cet-13508	128	11	glucanase	glucanase	ADJ
cet-13508	128	12	(	(	PUNCT
cet-13508	128	13	bgn16.1	bgn16.1	NOUN
cet-13508	128	14	)	)	PUNCT
cet-13508	128	15	from	from	ADP
cet-13508	128	16	the	the	DET
cet-13508	128	17	antagonistic	antagonistic	ADJ
cet-13508	128	18	fungus	fungus	NOUN
cet-13508	128	19	trichoderma	trichoderma	PROPN
cet-13508	128	20	harzianum	harzianum	ADJ
cet-13508	128	21	.	.	PUNCT
cet-13508	129	1	european	european	ADJ
cet-13508	129	2	journal	journal	PROPN
cet-13508	129	3	of	of	ADP
cet-13508	129	4	biochemistry	biochemistry	NOUN
cet-13508	129	5	265	265	NUM
cet-13508	129	6	,	,	PUNCT
cet-13508	129	7	145–151	145–151	NUM
cet-13508	129	8	.	.	PUNCT
cet-13508	130	1	https://doi.org/10.1046/j.1432-1327.1999.00698.x	https://doi.org/10.1046/j.1432-1327.1999.00698.x	PROPN
cet-13508	130	2	ferreira	ferreira	PROPN
cet-13508	130	3	-	-	PUNCT
cet-13508	130	4	lazarte	lazarte	PROPN
cet-13508	130	5	,	,	PUNCT
cet-13508	130	6	a.	a.	NOUN
cet-13508	130	7	,	,	PUNCT
cet-13508	130	8	gallego	gallego	NOUN
cet-13508	130	9	-	-	PUNCT
cet-13508	130	10	lobillo	lobillo	NOUN
cet-13508	130	11	,	,	PUNCT
cet-13508	130	12	p.	p.	PROPN
cet-13508	130	13	,	,	PUNCT
cet-13508	130	14	moreno	moreno	PROPN
cet-13508	130	15	,	,	PUNCT
cet-13508	130	16	f.j	f.j	PROPN
cet-13508	130	17	.	.	PROPN
cet-13508	130	18	,	,	PUNCT
cet-13508	130	19	villamiel	villamiel	NOUN
cet-13508	130	20	,	,	PUNCT
cet-13508	130	21	m.	m.	NOUN
cet-13508	130	22	,	,	PUNCT
cet-13508	130	23	hernandez	hernandez	PROPN
cet-13508	130	24	-	-	PUNCT
cet-13508	130	25	hernandez	hernandez	PROPN
cet-13508	130	26	,	,	PUNCT
cet-13508	130	27	o.	o.	PROPN
cet-13508	130	28	,	,	PUNCT
cet-13508	130	29	2019	2019	NUM
cet-13508	130	30	.	.	PUNCT
cet-13508	131	1	in	in	ADP
cet-13508	131	2	vitro	vitro	X
cet-13508	131	3	digestibility	digestibility	NOUN
cet-13508	131	4	of	of	ADP
cet-13508	131	5	galactooligosaccharides	galactooligosaccharide	NOUN
cet-13508	131	6	:	:	PUNCT
cet-13508	131	7	effect	effect	NOUN
cet-13508	131	8	of	of	ADP
cet-13508	131	9	the	the	DET
cet-13508	131	10	structural	structural	ADJ
cet-13508	131	11	features	feature	NOUN
cet-13508	131	12	on	on	ADP
cet-13508	131	13	their	their	PRON
cet-13508	131	14	intestinal	intestinal	ADJ
cet-13508	131	15	degradation	degradation	NOUN
cet-13508	131	16	.	.	PUNCT
cet-13508	132	1	j.	j.	PROPN
cet-13508	132	2	agric	agric	PROPN
cet-13508	132	3	.	.	PUNCT
cet-13508	132	4	food	food	NOUN
cet-13508	132	5	chem	chem	NOUN
cet-13508	132	6	.	.	PUNCT
cet-13508	133	1	67	67	NUM
cet-13508	133	2	,	,	PUNCT
cet-13508	133	3	4662–4670	4662–4670	NUM
cet-13508	133	4	.	.	PUNCT
cet-13508	134	1	https://doi.org/10.1021/acs.jafc.9b00417	https://doi.org/10.1021/acs.jafc.9b00417	X
cet-13508	134	2	gibson	gibson	PROPN
cet-13508	134	3	,	,	PUNCT
cet-13508	134	4	g.r	g.r	PROPN
cet-13508	134	5	.	.	PROPN
cet-13508	134	6	,	,	PUNCT
cet-13508	134	7	hutkins	hutkins	PROPN
cet-13508	134	8	,	,	PUNCT
cet-13508	134	9	r.	r.	PROPN
cet-13508	134	10	,	,	PUNCT
cet-13508	134	11	sanders	sanders	PROPN
cet-13508	134	12	,	,	PUNCT
cet-13508	134	13	m.e	m.e	PROPN
cet-13508	134	14	.	.	PROPN
cet-13508	134	15	,	,	PUNCT
cet-13508	134	16	prescott	prescott	PROPN
cet-13508	134	17	,	,	PUNCT
cet-13508	134	18	s.l	s.l	PROPN
cet-13508	134	19	.	.	PROPN
cet-13508	134	20	,	,	PUNCT
cet-13508	134	21	reimer	reimer	PROPN
cet-13508	134	22	,	,	PUNCT
cet-13508	134	23	r.a	r.a	PROPN
cet-13508	134	24	.	.	PROPN
cet-13508	134	25	,	,	PUNCT
cet-13508	134	26	salminen	salminen	PROPN
cet-13508	134	27	,	,	PUNCT
cet-13508	134	28	s.j	s.j	PROPN
cet-13508	134	29	.	.	PROPN
cet-13508	134	30	,	,	PUNCT
cet-13508	134	31	scott	scott	PROPN
cet-13508	134	32	,	,	PUNCT
cet-13508	134	33	k.	k.	PROPN
cet-13508	134	34	,	,	PUNCT
cet-13508	134	35	stanton	stanton	PROPN
cet-13508	134	36	,	,	PUNCT
cet-13508	134	37	c.	c.	PROPN
cet-13508	134	38	,	,	PUNCT
cet-13508	134	39	swanson	swanson	PROPN
cet-13508	134	40	,	,	PUNCT
cet-13508	134	41	k.s	k.s	PROPN
cet-13508	134	42	.	.	PROPN
cet-13508	134	43	,	,	PUNCT
cet-13508	134	44	cani	cani	PROPN
cet-13508	134	45	,	,	PUNCT
cet-13508	134	46	p.d	p.d	PROPN
cet-13508	134	47	.	.	PROPN
cet-13508	134	48	,	,	PUNCT
cet-13508	134	49	verbeke	verbeke	PROPN
cet-13508	134	50	,	,	PUNCT
cet-13508	134	51	k.	k.	PROPN
cet-13508	134	52	,	,	PUNCT
cet-13508	134	53	reid	reid	PROPN
cet-13508	134	54	,	,	PUNCT
cet-13508	134	55	g.	g.	PROPN
cet-13508	134	56	,	,	PUNCT
cet-13508	134	57	2017	2017	NUM
cet-13508	134	58	.	.	PUNCT
cet-13508	135	1	expert	expert	ADJ
cet-13508	135	2	consensus	consensus	NOUN
cet-13508	135	3	document	document	NOUN
cet-13508	135	4	:	:	PUNCT
cet-13508	135	5	the	the	DET
cet-13508	135	6	international	international	ADJ
cet-13508	135	7	scientific	scientific	ADJ
cet-13508	135	8	association	association	NOUN
cet-13508	135	9	for	for	ADP
cet-13508	135	10	probiotics	probiotic	NOUN
cet-13508	135	11	and	and	CCONJ
cet-13508	135	12	prebiotics	prebiotic	NOUN
cet-13508	135	13	(	(	PUNCT
cet-13508	135	14	isapp	isapp	ADJ
cet-13508	135	15	)	)	PUNCT
cet-13508	135	16	consensus	consensus	NOUN
cet-13508	135	17	statement	statement	NOUN
cet-13508	135	18	on	on	ADP
cet-13508	135	19	the	the	DET
cet-13508	135	20	definition	definition	NOUN
cet-13508	135	21	and	and	CCONJ
cet-13508	135	22	scope	scope	NOUN
cet-13508	135	23	of	of	ADP
cet-13508	135	24	prebiotics	prebiotic	NOUN
cet-13508	135	25	.	.	PUNCT
cet-13508	136	1	nat	nat	PROPN
cet-13508	136	2	rev	rev	PROPN
cet-13508	136	3	gastroenterol	gastroenterol	NOUN
cet-13508	136	4	hepatol	hepatol	ADJ
cet-13508	136	5	14	14	NUM
cet-13508	136	6	,	,	PUNCT
cet-13508	136	7	491–502	491–502	NUM
cet-13508	136	8	.	.	PUNCT
cet-13508	137	1	https://doi.org/10.1038/nrgastro.2017.75	https://doi.org/10.1038/nrgastro.2017.75	PROPN
cet-13508	137	2	kim	kim	PROPN
cet-13508	137	3	,	,	PUNCT
cet-13508	137	4	k.-h	k.-h	PROPN
cet-13508	137	5	.	.	PUNCT
cet-13508	137	6	,	,	PUNCT
cet-13508	137	7	kim	kim	PROPN
cet-13508	137	8	,	,	PUNCT
cet-13508	137	9	y.-w	y.-w	PROPN
cet-13508	137	10	.	.	PROPN
cet-13508	137	11	,	,	PUNCT
cet-13508	137	12	kim	kim	PROPN
cet-13508	137	13	,	,	PUNCT
cet-13508	137	14	h.b	h.b	PROPN
cet-13508	137	15	.	.	PROPN
cet-13508	137	16	,	,	PUNCT
cet-13508	137	17	lee	lee	PROPN
cet-13508	137	18	,	,	PUNCT
cet-13508	137	19	b.j	b.j	PROPN
cet-13508	137	20	.	.	PROPN
cet-13508	137	21	,	,	PUNCT
cet-13508	137	22	lee	lee	PROPN
cet-13508	137	23	,	,	PUNCT
cet-13508	137	24	d.s	d.s	PROPN
cet-13508	137	25	.	.	PROPN
cet-13508	137	26	,	,	PUNCT
cet-13508	137	27	2006	2006	NUM
cet-13508	137	28	.	.	PUNCT
cet-13508	138	1	anti	anti	ADJ
cet-13508	138	2	-	-	ADJ
cet-13508	138	3	apoptotic	apoptotic	ADJ
cet-13508	138	4	activity	activity	NOUN
cet-13508	138	5	of	of	ADP
cet-13508	138	6	laminarin	laminarin	ADJ
cet-13508	138	7	polysaccharides	polysaccharide	NOUN
cet-13508	138	8	and	and	CCONJ
cet-13508	138	9	their	their	PRON
cet-13508	138	10	enzymatically	enzymatically	ADV
cet-13508	138	11	hydrolyzed	hydrolyze	VERB
cet-13508	138	12	oligosaccharides	oligosaccharide	NOUN
cet-13508	138	13	from	from	ADP
cet-13508	138	14	laminaria	laminaria	PROPN
cet-13508	138	15	japonica	japonica	PROPN
cet-13508	138	16	.	.	PUNCT
cet-13508	139	1	biotechnol	biotechnol	PROPN
cet-13508	139	2	lett	lett	PROPN
cet-13508	139	3	28	28	NUM
cet-13508	139	4	,	,	PUNCT
cet-13508	139	5	439–446	439–446	NUM
cet-13508	139	6	.	.	PUNCT
cet-13508	140	1	https://doi.org/10.1007/s10529-005-6177-9	https://doi.org/10.1007/s10529-005-6177-9	PROPN
cet-13508	140	2	kumar	kumar	PROPN
cet-13508	140	3	,	,	PUNCT
cet-13508	140	4	k.	k.	PROPN
cet-13508	140	5	,	,	PUNCT
cet-13508	140	6	rajulapati	rajulapati	NOUN
cet-13508	140	7	,	,	PUNCT
cet-13508	140	8	v.	v.	ADP
cet-13508	140	9	,	,	PUNCT
cet-13508	140	10	goyal	goyal	PROPN
cet-13508	140	11	,	,	PUNCT
cet-13508	140	12	a.	a.	NOUN
cet-13508	140	13	,	,	PUNCT
cet-13508	140	14	2020	2020	NUM
cet-13508	140	15	.	.	PUNCT
cet-13508	141	1	in	in	ADP
cet-13508	141	2	vitro	vitro	X
cet-13508	141	3	prebiotic	prebiotic	ADJ
cet-13508	141	4	potential	potential	NOUN
cet-13508	141	5	,	,	PUNCT
cet-13508	141	6	digestibility	digestibility	NOUN
cet-13508	141	7	and	and	CCONJ
cet-13508	141	8	biocompatibility	biocompatibility	NOUN
cet-13508	141	9	properties	property	NOUN
cet-13508	141	10	of	of	ADP
cet-13508	141	11	laminari	laminari	ADJ
cet-13508	141	12	-	-	PUNCT
cet-13508	141	13	oligosaccharides	oligosaccharide	NOUN
cet-13508	141	14	produced	produce	VERB
cet-13508	141	15	from	from	ADP
cet-13508	141	16	curdlan	curdlan	NOUN
cet-13508	141	17	by	by	ADP
cet-13508	141	18	β-1,3	β-1,3	PROPN
cet-13508	141	19	-	-	PUNCT
cet-13508	141	20	endoglucanase	endoglucanase	NOUN
cet-13508	141	21	from	from	ADP
cet-13508	141	22	clostridium	clostridium	PROPN
cet-13508	141	23	thermocellum	thermocellum	PROPN
cet-13508	141	24	.	.	PUNCT
cet-13508	142	1	3	3	NUM
cet-13508	142	2	biotech	biotech	NOUN
cet-13508	142	3	10	10	NUM
cet-13508	142	4	,	,	PUNCT
cet-13508	142	5	241	241	NUM
cet-13508	142	6	.	.	PUNCT
cet-13508	142	7	https://doi.org/10.1007/s13205-020-02234-0	https://doi.org/10.1007/s13205-020-02234-0	NUM
cet-13508	142	8	kunze	kunze	PROPN
cet-13508	142	9	,	,	PUNCT
cet-13508	142	10	w.	w.	PROPN
cet-13508	142	11	,	,	PUNCT
cet-13508	142	12	1999	1999	NUM
cet-13508	142	13	.	.	PUNCT
cet-13508	143	1	technology	technology	NOUN
cet-13508	143	2	brewing	brewing	NOUN
cet-13508	143	3	and	and	CCONJ
cet-13508	143	4	malting	malting	NOUN
cet-13508	143	5	.	.	PUNCT
cet-13508	144	1	miller	miller	PROPN
cet-13508	144	2	,	,	PUNCT
cet-13508	144	3	g.l	g.l	PROPN
cet-13508	144	4	.	.	PROPN
cet-13508	144	5	,	,	PUNCT
cet-13508	144	6	1959	1959	NUM
cet-13508	144	7	.	.	PUNCT
cet-13508	145	1	use	use	NOUN
cet-13508	145	2	of	of	ADP
cet-13508	145	3	dinitrosalicylic	dinitrosalicylic	ADJ
cet-13508	145	4	acid	acid	NOUN
cet-13508	145	5	reagent	reagent	NOUN
cet-13508	145	6	for	for	ADP
cet-13508	145	7	determination	determination	NOUN
cet-13508	145	8	of	of	ADP
cet-13508	145	9	reducing	reduce	VERB
cet-13508	145	10	sugar	sugar	NOUN
cet-13508	145	11	.	.	PUNCT
cet-13508	146	1	anal	anal	ADJ
cet-13508	146	2	.	.	PUNCT
cet-13508	146	3	chem	chem	PROPN
cet-13508	146	4	.	.	PUNCT
cet-13508	147	1	31	31	NUM
cet-13508	147	2	,	,	PUNCT
cet-13508	147	3	426–428	426–428	NUM
cet-13508	147	4	.	.	PUNCT
cet-13508	148	1	https://doi.org/10.1021/ac60147a030	https://doi.org/10.1021/ac60147a030	PROPN
cet-13508	148	2	montero	montero	PROPN
cet-13508	148	3	,	,	PUNCT
cet-13508	148	4	m.	m.	NOUN
cet-13508	148	5	,	,	PUNCT
cet-13508	148	6	sanz	sanz	PROPN
cet-13508	148	7	,	,	PUNCT
cet-13508	148	8	l.	l.	PROPN
cet-13508	148	9	,	,	PUNCT
cet-13508	148	10	rey	rey	PROPN
cet-13508	148	11	,	,	PUNCT
cet-13508	148	12	m.	m.	NOUN
cet-13508	148	13	,	,	PUNCT
cet-13508	148	14	monte	monte	PROPN
cet-13508	148	15	,	,	PUNCT
cet-13508	148	16	e.	e.	PROPN
cet-13508	148	17	,	,	PUNCT
cet-13508	148	18	llobell	llobell	PROPN
cet-13508	148	19	,	,	PUNCT
cet-13508	148	20	a.	a.	NOUN
cet-13508	148	21	,	,	PUNCT
cet-13508	148	22	2005	2005	NUM
cet-13508	148	23	.	.	PUNCT
cet-13508	149	1	bgn16.3	bgn16.3	NOUN
cet-13508	149	2	,	,	PUNCT
cet-13508	149	3	a	a	DET
cet-13508	149	4	novel	novel	ADJ
cet-13508	149	5	acidic	acidic	ADJ
cet-13508	149	6	β-1,6	β-1,6	PROPN
cet-13508	149	7	-	-	NOUN
cet-13508	149	8	glucanase	glucanase	NOUN
cet-13508	149	9	from	from	ADP
cet-13508	149	10	mycoparasitic	mycoparasitic	ADJ
cet-13508	149	11	fungus	fungus	PROPN
cet-13508	149	12	trichoderma	trichoderma	PROPN
cet-13508	149	13	harzianum	harzianum	ADJ
cet-13508	149	14	cect	cect	NOUN
cet-13508	149	15	2413	2413	NUM
cet-13508	149	16	.	.	PUNCT
cet-13508	150	1	the	the	DET
cet-13508	150	2	febs	febs	NOUN
cet-13508	150	3	journal	journal	PROPN
cet-13508	150	4	272	272	NUM
cet-13508	150	5	,	,	PUNCT
cet-13508	150	6	3441–3448	3441–3448	NUM
cet-13508	150	7	.	.	PUNCT
cet-13508	150	8	https://doi.org/10.1111/j.1742-4658.2005.04762.x	https://doi.org/10.1111/j.1742-4658.2005.04762.x	PROPN
cet-13508	150	9	nelson	nelson	PROPN
cet-13508	150	10	,	,	PUNCT
cet-13508	150	11	d.l	d.l	PROPN
cet-13508	150	12	.	.	PROPN
cet-13508	150	13	,	,	PUNCT
cet-13508	150	14	cox	cox	PROPN
cet-13508	150	15	,	,	PUNCT
cet-13508	150	16	m.m	m.m	PROPN
cet-13508	150	17	.	.	PROPN
cet-13508	150	18	,	,	PUNCT
cet-13508	150	19	2008	2008	NUM
cet-13508	150	20	.	.	PUNCT
cet-13508	151	1	lehninger	lehninger	NOUN
cet-13508	151	2	principles	principle	NOUN
cet-13508	151	3	of	of	ADP
cet-13508	151	4	biochemistry	biochemistry	NOUN
cet-13508	151	5	,	,	PUNCT
cet-13508	151	6	4th	4th	ADJ
cet-13508	151	7	ed	ed	NOUN
cet-13508	151	8	.	.	PUNCT
cet-13508	152	1	palgrave	palgrave	PROPN
cet-13508	152	2	macmillan	macmillan	PROPN
cet-13508	152	3	.	.	PUNCT
cet-13508	152	4	soler	soler	PROPN
cet-13508	152	5	,	,	PUNCT
cet-13508	152	6	a.	a.	PROPN
cet-13508	152	7	,	,	PUNCT
cet-13508	152	8	de	de	PROPN
cet-13508	152	9	la	la	PROPN
cet-13508	152	10	cruz	cruz	PROPN
cet-13508	152	11	,	,	PUNCT
cet-13508	152	12	j.	j.	PROPN
cet-13508	152	13	,	,	PUNCT
cet-13508	152	14	llobell	llobell	PROPN
cet-13508	152	15	,	,	PUNCT
cet-13508	152	16	a.	a.	NOUN
cet-13508	152	17	,	,	PUNCT
cet-13508	152	18	1999	1999	NUM
cet-13508	152	19	.	.	PUNCT
cet-13508	153	1	detection	detection	NOUN
cet-13508	153	2	of	of	ADP
cet-13508	153	3	β-1,6	β-1,6	NOUN
cet-13508	153	4	-	-	ADJ
cet-13508	153	5	glucanase	glucanase	ADJ
cet-13508	153	6	isozymes	isozyme	NOUN
cet-13508	153	7	from	from	ADP
cet-13508	153	8	trichoderma	trichoderma	PROPN
cet-13508	153	9	strains	strain	NOUN
cet-13508	153	10	in	in	ADP
cet-13508	153	11	sodium	sodium	NOUN
cet-13508	153	12	dodecyl	dodecyl	PROPN
cet-13508	153	13	sulphate	sulphate	NOUN
cet-13508	153	14	–	–	PUNCT
cet-13508	153	15	polyacrylamide	polyacrylamide	NOUN
cet-13508	153	16	gel	gel	NOUN
cet-13508	153	17	electrophoresis	electrophoresis	NOUN
cet-13508	153	18	and	and	CCONJ
cet-13508	153	19	isoelectrofocusing	isoelectrofocuse	VERB
cet-13508	153	20	gels	gel	NOUN
cet-13508	153	21	.	.	PUNCT
cet-13508	154	1	journal	journal	NOUN
cet-13508	154	2	of	of	ADP
cet-13508	154	3	microbiological	microbiological	ADJ
cet-13508	154	4	methods	method	NOUN
cet-13508	154	5	35	35	NUM
cet-13508	154	6	,	,	PUNCT
cet-13508	154	7	245–251	245–251	NUM
cet-13508	154	8	.	.	PUNCT
cet-13508	155	1	https://doi.org/10.1016/s0167-7012(99)00021-4	https://doi.org/10.1016/s0167-7012(99)00021-4	NOUN
cet-13508	155	2	volkov	volkov	PROPN
cet-13508	155	3	,	,	PUNCT
cet-13508	155	4	p.v	p.v	PROPN
cet-13508	155	5	.	.	PROPN
cet-13508	155	6	,	,	PUNCT
cet-13508	155	7	rubtsova	rubtsova	PROPN
cet-13508	155	8	,	,	PUNCT
cet-13508	155	9	e.a	e.a	PROPN
cet-13508	155	10	.	.	PROPN
cet-13508	155	11	,	,	PUNCT
cet-13508	155	12	rozhkova	rozhkova	PROPN
cet-13508	155	13	,	,	PUNCT
cet-13508	155	14	a.m.	a.m.	ADV
cet-13508	155	15	,	,	PUNCT
cet-13508	155	16	sinitsyna	sinitsyna	NOUN
cet-13508	155	17	,	,	PUNCT
cet-13508	155	18	o.a	o.a	PROPN
cet-13508	155	19	.	.	PROPN
cet-13508	155	20	,	,	PUNCT
cet-13508	155	21	zorov	zorov	PROPN
cet-13508	155	22	,	,	PUNCT
cet-13508	155	23	i.n	i.n	PROPN
cet-13508	155	24	.	.	PROPN
cet-13508	155	25	,	,	PUNCT
cet-13508	155	26	kondratyeva	kondratyeva	PROPN
cet-13508	155	27	,	,	PUNCT
cet-13508	155	28	e.g.	e.g.	ADV
cet-13508	155	29	,	,	PUNCT
cet-13508	155	30	sinitsyn	sinitsyn	PROPN
cet-13508	155	31	,	,	PUNCT
cet-13508	155	32	a.p	a.p	PROPN
cet-13508	155	33	.	.	PROPN
cet-13508	155	34	,	,	PUNCT
cet-13508	155	35	2021	2021	NUM
cet-13508	155	36	.	.	PUNCT
cet-13508	156	1	properties	property	NOUN
cet-13508	156	2	of	of	ADP
cet-13508	156	3	recombinant	recombinant	ADJ
cet-13508	156	4	endo	endo	NOUN
cet-13508	156	5	-	-	PUNCT
cet-13508	156	6	β-1,6	β-1,6	NOUN
cet-13508	156	7	-	-	NOUN
cet-13508	156	8	glucanase	glucanase	NOUN
cet-13508	156	9	from	from	ADP
cet-13508	156	10	trichoderma	trichoderma	PROPN
cet-13508	156	11	harzianum	harzianum	ADJ
cet-13508	156	12	and	and	CCONJ
cet-13508	156	13	its	its	PRON
cet-13508	156	14	application	application	NOUN
cet-13508	156	15	in	in	ADP
cet-13508	156	16	the	the	DET
cet-13508	156	17	pustulan	pustulan	NOUN
cet-13508	156	18	hydrolysis	hydrolysis	NOUN
cet-13508	156	19	.	.	PUNCT
cet-13508	157	1	carbohydr	carbohydr	NOUN
cet-13508	157	2	res	re	VERB
cet-13508	157	3	499	499	NUM
cet-13508	157	4	,	,	PUNCT
cet-13508	157	5	108211	108211	NUM
cet-13508	157	6	.	.	PUNCT
cet-13508	158	1	https://doi.org/10.1016/j.carres.2020.108211	https://doi.org/10.1016/j.carres.2020.108211	PROPN
cet-13508	158	2	wang	wang	PROPN
cet-13508	158	3	,	,	PUNCT
cet-13508	158	4	s.	s.	PROPN
cet-13508	158	5	,	,	PUNCT
cet-13508	158	6	xiao	xiao	PROPN
cet-13508	158	7	,	,	PUNCT
cet-13508	158	8	y.	y.	PROPN
cet-13508	158	9	,	,	PUNCT
cet-13508	158	10	tian	tian	PROPN
cet-13508	158	11	,	,	PUNCT
cet-13508	158	12	f.	f.	PROPN
cet-13508	158	13	,	,	PUNCT
cet-13508	158	14	zhao	zhao	PROPN
cet-13508	158	15	,	,	PUNCT
cet-13508	158	16	j.	j.	PROPN
cet-13508	158	17	,	,	PUNCT
cet-13508	158	18	zhang	zhang	PROPN
cet-13508	158	19	,	,	PUNCT
cet-13508	158	20	h.	h.	PROPN
cet-13508	158	21	,	,	PUNCT
cet-13508	158	22	zhai	zhai	PROPN
cet-13508	158	23	,	,	PUNCT
cet-13508	158	24	q.	q.	PROPN
cet-13508	158	25	,	,	PUNCT
cet-13508	158	26	chen	chen	PROPN
cet-13508	158	27	,	,	PUNCT
cet-13508	158	28	w.	w.	PROPN
cet-13508	158	29	,	,	PUNCT
cet-13508	158	30	2020	2020	NUM
cet-13508	158	31	.	.	PUNCT
cet-13508	159	1	rational	rational	ADJ
cet-13508	159	2	use	use	NOUN
cet-13508	159	3	of	of	ADP
cet-13508	159	4	prebiotics	prebiotic	NOUN
cet-13508	159	5	for	for	ADP
cet-13508	159	6	gut	gut	ADJ
cet-13508	159	7	microbiota	microbiota	NOUN
cet-13508	159	8	alterations	alteration	NOUN
cet-13508	159	9	:	:	PUNCT
cet-13508	159	10	specific	specific	ADJ
cet-13508	159	11	bacterial	bacterial	ADJ
cet-13508	159	12	phylotypes	phylotype	NOUN
cet-13508	159	13	and	and	CCONJ
cet-13508	159	14	related	related	ADJ
cet-13508	159	15	mechanisms	mechanism	NOUN
cet-13508	159	16	.	.	PUNCT
cet-13508	160	1	journal	journal	NOUN
cet-13508	160	2	of	of	ADP
cet-13508	160	3	functional	functional	ADJ
cet-13508	160	4	foods	food	NOUN
cet-13508	160	5	66	66	NUM
cet-13508	160	6	,	,	PUNCT
cet-13508	160	7	103838	103838	NUM
cet-13508	160	8	.	.	PUNCT
cet-13508	161	1	https://doi.org/10.1016/j.jff.2020.103838	https://doi.org/10.1016/j.jff.2020.103838	PROPN
cet-13508	161	2	yang	yang	PROPN
cet-13508	161	3	,	,	PUNCT
cet-13508	161	4	s.	s.	PROPN
cet-13508	161	5	,	,	PUNCT
cet-13508	161	6	xiong	xiong	PROPN
cet-13508	161	7	,	,	PUNCT
cet-13508	161	8	h.	h.	PROPN
cet-13508	161	9	,	,	PUNCT
cet-13508	161	10	yan	yan	PROPN
cet-13508	161	11	,	,	PUNCT
cet-13508	161	12	q.	q.	PROPN
cet-13508	161	13	,	,	PUNCT
cet-13508	161	14	yang	yang	PROPN
cet-13508	161	15	,	,	PUNCT
cet-13508	161	16	h.	h.	PROPN
cet-13508	161	17	,	,	PUNCT
cet-13508	161	18	jiang	jiang	PROPN
cet-13508	161	19	,	,	PUNCT
cet-13508	161	20	z.	z.	PROPN
cet-13508	161	21	,	,	PUNCT
cet-13508	161	22	2014	2014	NUM
cet-13508	161	23	.	.	PUNCT
cet-13508	162	1	purification	purification	NOUN
cet-13508	162	2	and	and	CCONJ
cet-13508	162	3	characterization	characterization	NOUN
cet-13508	162	4	of	of	ADP
cet-13508	162	5	a	a	DET
cet-13508	162	6	novel	novel	ADJ
cet-13508	162	7	alkaline	alkaline	NOUN
cet-13508	162	8	β1,3	β1,3	PROPN
cet-13508	162	9	-	-	PUNCT
cet-13508	162	10	1,4	1,4	NUM
cet-13508	162	11	-	-	ADJ
cet-13508	162	12	glucanase	glucanase	ADJ
cet-13508	162	13	(	(	PUNCT
cet-13508	162	14	lichenase	lichenase	NOUN
cet-13508	162	15	)	)	PUNCT
cet-13508	162	16	from	from	ADP
cet-13508	162	17	thermophilic	thermophilic	ADJ
cet-13508	162	18	fungus	fungus	NOUN
cet-13508	162	19	malbranchea	malbranchea	PROPN
cet-13508	162	20	cinnamomea	cinnamomea	NOUN
cet-13508	162	21	.	.	PUNCT
cet-13508	163	1	journal	journal	PROPN
cet-13508	163	2	of	of	ADP
cet-13508	163	3	industrial	industrial	ADJ
cet-13508	163	4	microbiology	microbiology	NOUN
cet-13508	163	5	and	and	CCONJ
cet-13508	163	6	biotechnology	biotechnology	NOUN
cet-13508	163	7	41	41	NUM
cet-13508	163	8	,	,	PUNCT
cet-13508	163	9	1487–1495	1487–1495	NUM
cet-13508	163	10	.	.	PUNCT
cet-13508	164	1	https://doi.org/10.1007/s10295-014-1494-4	https://doi.org/10.1007/s10295-014-1494-4	NUM
cet-13508	164	2	108	108	NUM
cet-13508	164	3	5klemanska.pdf	5klemanska.pdf	NUM
cet-13508	164	4	purification	purification	NOUN
cet-13508	164	5	and	and	CCONJ
cet-13508	164	6	characterisation	characterisation	NOUN
cet-13508	164	7	of	of	ADP
cet-13508	164	8	a	a	DET
cet-13508	164	9	fungal	fungal	ADJ
cet-13508	164	10	β-1,6	β-1,6	NOUN
cet-13508	164	11	-	-	NOUN
cet-13508	164	12	glucanase	glucanase	NOUN
cet-13508	164	13	for	for	ADP
cet-13508	164	14	the	the	DET
cet-13508	164	15	production	production	NOUN
cet-13508	164	16	of	of	ADP
cet-13508	164	17	gentio	gentio	NOUN
cet-13508	164	18	-	-	PUNCT
cet-13508	164	19	oligosaccharides	oligosaccharide	NOUN
cet-13508	164	20	from	from	ADP
cet-13508	164	21	brewer	brewer	NOUN
cet-13508	164	22	’s	’s	PART
cet-13508	164	23	spent	spend	VERB
cet-13508	164	24	yeast	yeast	NOUN