





































Clinical Medicine Insights
Received 05 Feb 2022 | Revised 08 Feb 2022 | Accepted 14 Feb 2022 | Published Online 16 Feb 2022

DOI: https://doi.org/10.52845/CMI/2022-3-1-3 
CMI 03 (01), 256−261 (2022) ISSN (O) 2694-4626 IF:1.6

RESEARCH ARTICLE

Environmental Problem Solution

Michael Vladislavovich Tyurin M.D., Ph.D
1the CEO, Executive Chairman of
Microbial Biocatalyst
International, Inc. and
Inorgcarbdiesel, Inc. P. O. Box
300230, Houston, TEXAS, the
USA.

Abstract
We describe herein our corporate experience of use of both carbon
negative the gasoline replacement fuel isobutanol and the diesel fuel
replacement fuel diacetyl alcohol in the engines of the sacrificed for this
test corporate cars 2020 Toyota Camry and 2020 Porshe Cayenne Turbo
(gasoline replacement) and 2020 Shevrolet Silverado 1500 (diesel fuel
replacement). Gasoline fuel replacements were used for 20,000 miles
and the diesel fuel replacement was used for 10,000 miles. The certified
mechanics invited from the respective cars dealerships did not find
no any deviations in said cars performance and no any gaskets leaks
creating further problems in said cars. We suggest that our carbon
negative gasoline and diesel fuel replacements will replace initially the
gasoline and the diesel fuel nationwide and then world-wide.

Copyright : © 2022 The Authors. Published by Publisher. This is an
open access article under the CC BY-NC-ND license
(https://creativecommons.org/licenses/by-nc-nd/4.0/).

1 INTRODUCTION

We presently are in the desperate need to
find the good our corporate investment
opportunity to invest in our own gaso-

line stations to sell the fuel isobutanol and the
fuel diactetyl alcohol as the replacements of the
gasoline and the diesel fuel both originating from
petroleum distillation, respectively. The refineries,
80 petroleum refineries in the Continental US, pro-
ducing a lot of air CO2 as the mandatory byproduct
of petroleum distillation, the petroleum distillation
on the petroleum fractions with different content
of the carbohydrate molecules C7 to C9 for the
manufacture of gasoline and C10-C15 for the diesel
fuel manufacture there is the now mandatory need

to find the replacements of the gasoline and the
diesel fuel from said petroleum distillation. With
the petroleum-based gasoline and diesel fuel there
is always the problem of polluting the environment
during the petroleum distillation process, necessary
to get the fractions of petroleum originated gaso-
line and petroleum originated diesel fuel, and more
the air CO2 contamination during burning of said
gasoline and diesel fuel. Even NASA in 2010 after
bombarding the Earth satellite Moon and finding
ice on the dark Moon surface stated that the Earth
has passed the point of no return to the natural
environmental conditions of the pre petroleum use
era of the year 1900 [1]. Using of gasoline or diesel
fuel emits even more air CO2. You remember the
US families where each kid at the age 16 wants to

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https://doi.org/10.52845/CMI/2022-3-1-3
https://creativecommons.org/licenses/by-nc-nd/4.0/


MANUSCRIPT CENTRAL
M.D., PH.D

have their own car despite he or she has brothers or
sisters. In 2010 NASA has claimed that our planet
Earth has reached the point of no return because
the air CO2 level has reached in 2010 the mark of
400 ppm. Now it is above 450 ppm and growing
above that. What specifically the air CO2 does to
the environment? The CO2 is among the heaviest
gases of the air blend, reaching its density of 1.997
g/m3. As such, the air CO2 under no wind conditions
spreads on the ground surface. CO2 is known as
the natural absorbent of the Solar infrared energy.
Distributing over the ground CO2 gets very hot due
to the Solar infra red energy adsorption and heats the
ground. It has been noted, that in the Southern US the
ground temperature might exceed the water boiling
temperature under the normal atmospheric pressure
conditions. The ground is the main refuge for all
the fresh water: water coming from rains and water
coming from melting snow and ice. From the ground
where it is collected the fresh water goes under the
constant Earth gravity to feel streams, rivers and
lakes. If the fresh water gets boiled in the ground,
then less fresh water goes to the rivers and lakes.
Not only boiling leads to the fresh water evaporation
under the constant Earth gravity force [1] This is the
huge current environmental problem, identified by
NASA in 2010 and noted by the Author of this article
in the year of 2000, when he worked at Celanese
Chemicals dealing with the acetic action production
by themicroorganismsActetogenswho are themajor
air CO2 absorbers to produce acetate via the acetyl-
CoA pathway [1].

The Fig. 1 shows the effect of air CO2 on the Earth
environment.

Supplementary information The online version of
this article (https://doi.org/xx.xxx/xxx.xx) contains
supplementary material, which is available to autho-
rized users.

Fig. 1. The negative environmental effect of the
increased air CO2 content [1].

Evaporated fresh water gets in the Earth atmosphere,
and from the Earth atmosphere comes to the Space
vacuum surrounding our planet. In 2010 NASA has
found fresh water frozen as ice on the dark Moon
surface. We might need to remind the readers that
Moon is located in 220,000 miles away from Earth
surface. So, the Universe vacuum sucks fresh water
from Earth indefinitely and the dark surface of the
Moon worked as the vacuum trap for said fresh water
vapors from the Earth. We on the Earth produce the
air CO2, more and more every year, facilitating the
loss of the fresh Water to the outer Space.
The most advanced Earth venture capitalists already
have started testing of the new Space travel technol-
ogy intended for the search of the new planets for
the relocation of the overcrowded Earth population
as the practical solution of the outlined above envi-
ronmental problem Earth faces currently. However,
despite of the venture capitalist investments, the level
of the technology used still does not allow the con-
struction of the efficient outer Space transportation
vehicles.
We are herein, on the planet Earth, and we de-
velop the carbon negative technologies of gasoline
and diesel fuel manufacture from the concentrated
air CO2. We have already developed Acetogens-
biocatalysts for the efficient continuous fermentation
of the gas blends composed from the concentrated air
CO2 and Nitrogen, also present in the atmospheric
air for above 70 %. The third component of said gas

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ENVIRONMENTAL PROBLEM SOLUTION

blend is Hydrogen, H2. We have postulated our en-
vironmentally free mission to produce the hydrogen
by the sea water distillation using the free electricity
obtained using the modern solar panels [5-7]. The
Equator area is the best for the solar energy collection
using the solar panels. The level of Solar irradiation
at the Equator area exceeding 11,000 miles around
the Earth, is 1,330 Wt/m2 of solar panels/hour. Basi-
cally, we are thinking of growing the separate line of
the private investment to organize themanufacture of
the Solar panel boats, then distributed in the Ocean
around the Equator. We are still in the process of
determining how much that will cost to produce a
particular Solar panel boat, as it has to distill the sea
water 24/7/365. Said floating Solar panel boats with
the sea water distillation capability have to have the
alkaline batteries currently available in the multitude
at the disposing the used submarines shipyards in the
USA. Due to the restricted nature of said shipyards
information, the process is somewhat complicated,
but we are inspired by our direct desire and our
corporate mission to establish the reliable network
of floating sea water distillers, packing said distilled
sea water in the regular water bottles for the selling to
the Earth customers. This is the second major solu-
tion of the environmental problem the overcrowded
Earth currently has, beyond the relocation of the
Humankind to the other planets. The relocation is
great but has multiple problems not only related
to the Spacecraft creation but also to the potential
danger of the new planets environment for the relo-
cating people. We are working also in this direction
trying to create de novo the ways to distribute the
recombinant vaccine proteins in the human bodies
[13]. Unfortunately current technologies used by the
gene transfer corporations do not allow the health
safe systems for the recombinant protein distribution
to the human bodies [2-4]. Our publication shows
this our intent acting in the form of the publication
of the peer reviewed articles instead of filing the US
patents due to the limits we do currently have with
the investor funding [2-7].
If the author may come back to his fuel isobutanol
as the carbon negative gasoline replacement, the
network of the biochemical reactions leading from
the atmospheric CO2 and the atmospheric nitrogen
to the fuel isobutanol is the following, as shown in

Fig. 2. We also have used the hydrogen obtained by
the solar power distillation of the freely available sea
water of the Mexican Gulf. The manufacturing cost
of the fuel isobutanol in our hands was $0.35 per
gallon of product.

Fig. 2. Fuel Isobutanol produced from the air with
the addition of hydrogen obtained via solar panels
powered electrolysis of the sea water [1].
Let’s go to the fuels section of this article. Isobutanol
fuel as the carbon negative gasoline replacement
was used in the gasoline engines of our corporation
freely subjected to this fuel tests. Since n-butanol
became the fuel gasoline replacement of the future
[12]. Fuel Isobutanol was also recommended as the
part of the fuel mixture for the airjet turbines by
the Gevo Inc. [8]. In the field of fuel butanol iso-
mers our current competitors include only Butamax
(DuPont enterprise). In the field of the existing gaso-
line market (over $1 trillion) or competitors are all
international petroleum corporations like SHELL,
BP, etc. We offer herein our carbon negative fuel
isobutanol manufactured from the air CO2 for only
$0.35 per gallon. We describe in this article our
corporate experience of use of both carbon nega-
tive the gasoline replacement fuel isobutanol and

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M.D., PH.D

the diesel fuel replacement fuel diacetyl alcohol in
the engines of the sacrificed for this test corporate
cars 2020 Toyota Camry and 2020 Porshe Cayenne
Turbo (gasoline replacement, 20,000 for each said
vehicle) and the diesel fuel replacement fuel diactyl
alcohol tested was the sacrificed by our corporations
2020 Shevrolet Silverado 1500 (10,000 miles for
the last cartesting).. We are not in the position to
disrupt the fuel market structure at this point of our
corporate funding, and at the price ranks we facing
currently we are not going to do so. To stay herein
on the existing fuel market with our carbon negative
gasoline replacement the fuel Isoburanol we offer
the free foods for the amount of the fuel isobutanol
purchase to every customer at our future gasoline
stations. This way we plan to be very profitable. Re-
member, the Author was almost killed for his intent
to commercialize his fuel isobutanol manufacture
technology at the international corporation ******,
and after that, with the Houston Police report of not
guilty for said the Author’s corporate car totaling by
the Toyota Tundra truck in Houston TEXAS, which
has crossed the Club Creek drive where the Author
drove straight the Author could not get any feedback
from the Houston FBI for the investigation of his
attempted murder as it stands now in 2021, however
the statute of limitations for the attempted murder
in the USA is 20 years. The only drawback of the
fuel isobutanol is the increase of its viscosity in the
fuel line at negative surrounding air temperatures. To
overcome this shortcoming of the fuel isobutanol for
its use at the low temperatures we offer it not in the
pure form as it has been suggested before [8], but as
the mixture with the gasoline at the ratio 80% fuel
isobutanol and 20% of gasoline. Of course, in the
Southern States of the USA the 100% fuel isobutanol
can and has to be used with no problems due to its
higher viscosity at the low air temperatures almost
around the year long: from February-March through
the end of November. During short winter time we
offer for sale the gasoline fuel isobutanol/gasoline
mixture as described above.
Now, let’s go back to the carbon negative manufac-
ture of the carbon negative diesel fuel manufacture.
The Author has invented and published in the peer-
reviewed journal and herein distributes his technol-
ogy of the diesel fuel manufacture from the air CO2

[8]. Basically the biochemical network of the related
to the carbon negative manufacture of the carbon
negative diesel fuel is as shown in the Fig. 3.

Fig. 3. Shematic network of the biochemical reac-
tions of the acetone production from air CO2[10].
The produced acetone was condensed to the diacetyl
alcohol per the US Patent US1550792A [11].
The manufacturing cost of 1 gallon of the carbon
negative fuel diactetyl alcohol is only $0.37 while
Shell, BP and other petroleum corporations spend
over $1.70 per diesel fuel gallon manufacture at
the petroleum refineries {1}. The Author describes
herein the results of his personal use of the fuel di-
acetyl alcohol solely in his corporate 2020 Shevrolet
Silverado 1500 truck with no attempts to convey said
personal experience on the other makes of the diesel
fuel trucks and other diesel fuel engines. Over the
10,000 miles run, the 2020 Shevrolet Silverado 1500
truck mileage was achieved on the highway at the
average mileage ~35 mpg, and at ~24 mpg driving in
the city. That was the new 2020 corporate Shevrolet
Silverado 1500 truck with the diesel engine.With the
older models of the diesel trucks these numbers will
be most likely lower.We did not notice no side effect
of using the diacetyl alcohol instead of the regular
diesel fuel as the diesel truck engine and the truck
was checked by the Chevrolet mechanic, with the
only exception that we had to keep in memory the
need to feel the truck with the fuel only at the corpo-
rate territory, not at the regular gas station. Probably,
at this point we will offer the fuel diacetyl alcohol
to the local gas stations as the fuel for the diesel

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ENVIRONMENTAL PROBLEM SOLUTION

engines or will have our own carbon negative diesel
fuel stations around the Nation.. However, they will
have to test all the safety parameters of storage of
this fuel on the station and the details of its use by
the customers if anything unusual comes up. If we are
successful in all said tests, the satisfactory results will
go for the National/World’s implementation of our
new diesel fuel and to the new gasoline stations, sell-
ing only diacetyl alcohol to customers as the diesel
fuel at the same sales cost, as there is no our intent to
disrupt the fuel market by the sudden decrease of the
fuel prices. Again, in this case we will offer to the
customers purchased our carbon negative diactetyl
alcohol the food for the amount they spent on the
diesel fuel. .Ourmajor concern herein is related to the
use of our gasoline and the diesel fuel replacements
to help the air jet aviation to help to fight the air CO2

production. We are not aware of any competition on
the carbon negative diesel fuel market at this time,
but the market competition will be with the major
international petroleum corporations like Shell, BP
and others.

2 DECLARATIONS:

Ethical Approval and Consent to Participate. This
article does not contain any section, requiring Ethical
Approval. The only Author of this article is complied
this section with the Consent to Participate. Consent
to Participate. The Author complied with the consent
to publish this article.
Consent to Publish. The Author complied with the
Consent to Publish this original article. Authors Con-
tribution. Dr. Michael V. Tyurin has planned all the
experimental work, conducted all the experiments,
analyzed the experimental data, wrote this original
manuscript, edited ot as appropriate and submitted
for this publication the edited original manuscript.
FUNDING was done by the private investors, who
declined to provide their names and their business
affiliations. The investors noted, the author should
decline any source of funding. Competing Interests.
The Author does not have any competing interests
with any third party. The author declares his personal
conflict of interests with the law firm in Houston, TX

Hirsch andWestheimer, which has destroyed his cor-
porate website http:syngasbiofuelsenergy.com, and
with the major petroleum and gasoline/diesel fuel
companies in Houston (TX), with the Houston Police
(City of Houston) and with the Houston FBI ignoring
the Author’s concern about his attempted murder
committed by SHELL after the Author has presented
to SHELL his proprietary technology for gasoline
manufacture from the air CO2, not from petroleum
(Tyurin MV, et al. 2019) The attempted murder is
of no interest for the corrupt by the major petroleum
corporations in Houston, TX Houston FBI. The Au-
thor has no intent to file the lawsuit against the
Houston FBI at any point, but he is inclined to make
this case the public domain. Houston FBI does not
follow the established in the USA Federal Laws.
Availability of data and materials, All the data and
materials are available if necessary from the Author
of this manuscript.
Authors’ information The Author is the owner of
his mentioned TEXAS businesses Microbial Bio-
catalyst International, Inc. and Inorgcarbdiesel, Inc.
The work has been done at the corporate site with
the USPS address P. O. Box 300230, Houston, TX
77230.

3 REFERENCES

1.http://syngasbiofuelsenergy.com
2.Tyurin MV (2021) Cure of the Intestinal Disorders
(recombinant pancreatic lipase expression at intesti-
nal bifidobacteria). Medical & Clinical Research
6(12):788-794.
3.Tyurin MV (2021) Expression in Situ of the
Recombinant Human Erythropoetin and Recom-
binant In-Sulin. J Diabetes Metab. 12:900. doi:
10.35248/2252-5211.21.12.900
4.Tyurin MV (2021) Successful Treatment of
Diabetes II in adult patient and New Prospects of
Recombinant Vaccine and Recombinant Proteins
Engineering in situ. J Diabetes Metab. 12:871-875.
5.Tyurin MV 2021. Gasoline Replacement Fuel Di-
acetyl Alcohol. International Journal of Automotive
Technology. Submitted.
6.Tyurin MV 2021. Air CO2 for the Manufacture
of the Commodity Fuels. Atmospheric Pollution

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M.D., PH.D

Research, Submitted October 2021.
7.Tyurin MV 2021. Diacetone Alcohol as the Diesel.
Energy Efficiency. Energy Efficiency. Ed.: Dr.
Muhammad Wakil Shahzad. IntechOpen. Rijeka:
Janeza Trdine 9, 51000 Rijeka, Croatia. London: 5
Princes Gate Court, London, SW7 2QJ, UK. ISBN
978-1-83969-828-6.
8.Berzin V, Kiriukhin M, Tyurin M. (2012)
Selective production of acetone during contin-
uous synthesis gas fermentation by engineered
biocatalyst Clostridium sp. MAceT113. Letters of
Appl Microbiol. 55(2):149-54. doi: 10.1111/j.1472-
765X.2012.03272.x.
9.Glen Johnston. Alcohol to Jet-Isobutanol. PDF of
the PPT Presentation. Expect for Future Develop-
ment and production of Aviation Alternative Fuel.
2017 ICAO Seminal on Alternative Fuels. ICAO
Headquarters, Montreal, 8-9 February 2017.
10.Berzin V, Kiriukhin M, Tyurin M. (2012)
Selective production of acetone during contin-

uous synthesis gas fermentation by engineered
biocatalyst Clostridium sp. MAceT113. Letters of
Appl Microbiol. 55(2):149-54. doi: 10.1111/j.1472-
765X.2012.03272.x.
11.US Patent US1550792A.
12.Tyurin MV, Padda R, Huang K-X, Wardwell
S, Caprette D, Bennett GN (2000) Electrotrans-
formation of Clostridium acetobutylicum ATCC
824 using high-voltage radio frequency modulated
square pulses // J Appl Microbiol. 88(2):220-227.
13.Tyurin MV. (2021) Environmental Problem
Solution. Submitted for purlication.

How to cite this article: M.D., Ph.D M.V.T.
Environmental Problem Solution. Clinical
Medicine Insights. 2022;256−261. https://doi.or
g/10.52845/CMI/2022-3-1-3

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	Introduction
	DECLARATIONS: 
	References

