








































CMI 05 (02), 600−618 CMI JOURNAL 600

Clinical Medicine Insights 

DOI:https://doi.org/10.52845/CMI/2023-5-2-1
CMI 05 (02), 600-618 (2023) ISSN (O) 2694-4626

ORIGINAL ARTICLE

Carbon Negative Gasoline and Diesel Fuel Replacements

Dr. Michael Vladislavovich Tyurin

*Corresponding Author: Dr. Michael Vladislavovich Tyurin

Introduction

Recent changes at the top of the US Executive 

administration have been combined with the not 

very wise decisions to cut down the petroleum 

imports from the Russian Federation.  The free 

market economy of the US  immediately reacted 

by skyrocketing of the Gasoline and the Diesel 

fuels  prices at the gas stations to the levels 

affecting all other prices nationwide.  For instance 

the cost of the TX DL skyrocketed from $11 USD 

to $33 USD (the coefficient of recalculation all the 

prices this year vs. year of 2021 and below ghat 

year is up to 3 and above - the rule of the market 

economy).  That coefficient 3 and above 3 is 

typical for the increase of other prices Nationwide 

as the major price-wise rule of the free market 

economy [21].  Subsequently, the proper running 

of the US economy is possible only with the very 

wise decisions of the top of governmental 

administration.  We offer herein the carbon 

negative replacements of the Gasoline and of the 

Diesel fuel manufactured from petroleum by the 

same fuels manufactured from the air CO2.  

Nationwide replacement of the technologies based 

on the use of petroleum as the source of chemicals 

or the fuels manufactured by the carbon negative 

technologies is absolutely essential since the Earth 

has passed the level of the air CO2 400 ppm, the 

“Point of No Return” per the NASA discoveries of 

2010 [21].  Now the air CO2 exceeds 450 ppm 

51.and goes up based of the wide distribution of

the intensive transportation based on the use of 

petroleum-derived Gasoline and Diesel fuel [21].  

The Author has written much about the Carbon 

Negative replacements of Gasoline or Diesel fuel 

manufactured by international petroleum 

corporations from petroleum via its distillation at 

the refineries the number of which is around 80 in 

the continental US and each of them operating 

produces a lot of air CO2 [5-16,19].  Now there is 

the time to elaborate this topic.   

Abstract:  
Petroleum recovery from the Ground and its distillation to get Gasoline and Diesel fuel burned in 

Gasoline and Diesel cars / trucks, airjets, etc. leads to the Global Environmental Catastrophe in 10-15 

years from now which the humankind will not survive.  The Author plans to save the Earth’s fresh water 

by developing Nationwide manufacture of the Carbon Negative replacements of Gasoline and Diesel fuel 

and add additional distilled water and the genetically engineered foods to help the humankind pass said 

.environmental crisis.  The Author plans to raise additional investor’s money to organize Nationwide 

network of the new type of gas stations selling not only Carbon Negative fuels but additionally to that the 

foods for home cooking and essential household items to save time to the valued customers preventing 

them to drive additionally to Walmart and grocery stores. The ordering system to provide additional goods 

is also planned. The environmental problem outlined has the solution: elimination of international 

petroleum corporations and their change to Carbon Negative corporations. 

Key words:  Carbon Negative fuels; additional distilled water from sea water; genetically engineered 

Carbon Negative foods; new type of the gas stations; the ordering system for the new type of gas stations. 

Copyright : © 2021 The Authors. Published by Medical Editor and Educational Research Publishers Ltd. 

This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/lic enses/by-

nc-nd/4.0/). 

Received 25 Jan 2024 | Revised 10 Feb 2024 | Accepted 25 Mar 2024 | Published Online 15 Apr 2024

https://creativecommons.org/lic%20enses/by-nc-nd/4.0/
https://creativecommons.org/lic%20enses/by-nc-nd/4.0/


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The combustion energy of fuel butanol is 

represented by the following numbers for the 

isomers of fuel n-Butanol shown below as 

indicated in [6,7]: 1-Butanol: −2.671 kJ/mol, 2-

Butanol: −2.661.1 kJ/Mol, 62.Isobutanol:

−2.662.6 kJ/Mol, Tert-butyl alcohol: −2.644.8 

kJ/Mol. The combustion energy of Gasoline is 

slightly higher. It is around -3.200kJ/Mol 64.[6]. 

The combustion energy of DAA or Mesityl oxide 

is -3.600 kJ/Mol [6]. In addition to that the octane 

number of the most prosperous fuel Isobutanol is 

98 [1,2]. Therefore, adding the fuel Isobutanol 

[18] to the mixture of other Carbon Negative fuels 

as above will substantially increase the overall 

summary octane number of said mixture. Besides 

that the viscosity of pure fuel Isobutanol and other 

isomers of fuel n-Butanol prevents it safe use at 

outside temperatures below 34
o
F [21].  That is

why we offer this Carbon Negative mixture 

comprising 40% of fuel Isobutanol, 10% fuel n-

Butanol, 72.30%  Mesityl oxide [24,34,35] and 

20% fuel Diacetyl alcohol (DAA) [15-17, 73.29].  

This will eventually compete with the products of 

the international petroleum corporations 

originated from petroleum as we discuss below its 

causing the outlined environmental crisis related 

to the increase of the air CO2 content [24]. 

The Mesityl oxide chemical formula is give in 

Fig. 1. 

Fig. 1.  Mesityl oxide [24,34,35]. 

Therefore, we have decided to sacrifice both 

corporate cars for the test driving them on the 

highway and in the city of Brownsville, TEXAS, 

to see if any damage could possibly happen to the 

cars, and especially their engines operated with 

the fuel isobutanol and other carbon negative fuels 

as above. The manufacturing price of the acetone 

manufactured from the air CO2 is approximately 

$0.35 per gallon [3]. The condensation of the two 

acetone molecules over the alkaline catalyst per 

[4] gives fuel Diacetyl alcohol at the 

manufacturing cost of $0.02 per gallon. The 

combustion energy of the fuel Diacetyl alcohol is 

approximately -3.473 kJ/Mol as compared to the 

combustion energy of the Diesel fuel of -808 

kJ/Mol which substantially exceeds the typical for 

the diesel fuel combustion engine energy release 

as the mechanical energy of driving and 

corresponds to the higher engine power under the 

given engine mechanical dimensions, probably 

because of the increase of the combustion mixture 

explosive pressure. Mesityl oxide combustion 

energy elevates that of the Diesel fuel, and we 

have decided to test the said mixture of the 

Carbon Negative fuels comprising as above  40% 

of fuel Isobutanol, 10% fuel n-Butanol, 30%  

Mesityl oxide [24,34,35] and 20% fuel Diacetyl 

alcohol (DAA) and that 98.mixture has been 

tested on the Diesel truck 2022 RAM 3500. 

However, we are not the explosion experts to 

discuss this herein [5].  Indicated elevation of the 

explosive pressure in the case of using Diacetyl 

alcohol (DAA) and Metsityl oxide happens in the 

purchased manufactured diesel vehicles.  This 

note is to keep checking the engine combustion 

chamber gaskets often to notice any damage by 

the qualified dealership mechanic or to notice 

sudden loss of engine power with the need to get 

the engine to the qualified dealership mechanic for 

repairs of damaged combustion chamber gaskets 

[6]. 

Biocatalysts used to manufacture the Carbon 

Negative fuels have different synthetic genes 

introduced by the means of genome tailoring 

using the unique Electrotrasformation / 

Electrofusion Generator developed by the Author 

when he still was in the Russian Federation 

[18,19]. Four carbon alcohol fuel n-Butanol and 

its isomers are widely recommended to replace 

Gasoline manufacture by the international 



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CMI JOURNAL  Dr. Michael Vladislavovich Tyurin 

petroleum corporations from petroleum.  Said isomers of fuel n-Butanol are shown in Fig. 2.

Fig. 2.  Fuel n-Butanol and its isomers sec-Butanol, tert-Butanol and Isobutanol [20]. 

Our biocatalyst making fuel Isobutanol has several 

genetically engineered genes for that purpose 

[18,20].  The fuel Isobutanol bioisynthesis by this 

strain is shown in Fig. 3 

Fig. 3.   Genetically engineered fuel Isobutanol pathway in our biocatalyst [18,20]. 



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124.Fuel Isobutanol has elevated viscosity and at 

lower temperatures near O 
o 

C and below that 

forms gels [21].  It is obvious that any gel 

structure in the fuel line at said low outside 

temperatures will be the obstacle for the proper 

internal combustion engine  operations. Therefore 

we offer the blends of 80 % (vol/vol) carbon 

negative Isobutanol manufactured by our carbon 

negative corporations and 20% (vol/vol) of 

Diacetone Alcohol manufactured by our carbon 

negative corporations, etc.  Said mixture of the 

Carbon Negative fuels as above survives the 

negative temperatures without the increase of the 

viscosity in the fuel line and therefore, this 

mixture is totally compatible with the Diesel and 

Gasoline using trucks nationwide, including the 

territories where the low winter and Spring / 

Autum temperatures exist Nationwide. Said 

mixture of Carbon Negative fuels maintains liquid 

state even at very low negative (much below 0
o
C)

temperatures and does not form any gel structures 

in the fuel line ensuring the normal operation of 

the engine. 

Fuel Isobutanol, the perfect Carbon Negative fuel, 

has a shortcoming bringing it to the zero use:  

high viscosity, tendency to form gel at 

temperatures below 0
o
C (or 34

o
F) [22,23].  The

fuel Isobutanol has viscosity cP or mPa•s @ 20°C 

3.102 [24].  Forming gel in the fuel line is 

absolutely not acceptable.  The engines have to 

run perfectly at any reasonable temperatures.  If in 

the State of TX the low temperatures are typical 

for only a few months, then in the Northern States 

the winter has low air temperatures for a much 

longer time.  Therefore, there has to be the tool to 

eliminate the Gasoline viscosity to the levels 

acceptable at the low air temperatures below 0
o
C.

This remedy is found already.  Mixing of 80 % 

(vol/vol) of Carbon Negative fuel Isobutanol with 

20 % (vol/vol) of Diacetyl Alcohol (DAA), which 

we produce Carbon Negative from condensing of 

the two Carbon Negative acetone molecules over 

the alkalyne catalyst [25,26]. Let me go to the 

carbon negative fuels - Carbon Negative 

replacements of Gasoline and Diesel fuel 

manufactured from petroleum by the international 

petroleum corporations.  Earlier we have 

published the Diacetone Alcohol (DAA) as the 

carbon negative replacement of the Gasoline and 

Diesel fuel.  DAA might be produced by the 

condensation of the two Acetone molecules over 

the alkaline catalyst [4].  The explosion energy of 

DAA  is way above the explosion energy of the 

Diesel fuel manufactured by the major 

international petroleum corporations from the 

petroleum [27,28].  Therefore, we have to pray 

that our customers will check their engines 

regularly with the qualified mechanics for the 

damages which potentially might be caused by the 

use of DAA in the existing trucks. Therefore the 

manufacturers of the diesel trucks will have to 

make certain changes to the design of their trucks 

to accommodate much higher explosion energy of 

added part of DAA.  This might be unnecessary 

however their manufacturers have to acknowledge 

the massive use of the carbon negative Gasoline 

replacement and make certain changes improving 

the mechanical resistance of the gaskets in the 

engine compartment.  Not to mention herein that 

the international petroleum corporations now 

spend $1.75 to manufacture 1 gallon of Gasoline 

or 1 gallon of the Diesel fuel.  That eventually 

affects the prices [of the Gasoline and the Diesel 

fuel manufactured from petroleum [6]. 

Fig. 4 explains how our Acetogen biocatalyst 

synthesized Carbon Negative fuel Acetone from 

the air CO2 [29].  Resulting Carbon Negative fuel 

Acetone is used for the manufacture of DAA by 

the condensation of the two Acetone molecules 

over the alkaline catalyst [4].



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Fig. 4.  Biosynthesis of fuel Carbon Negative acetone from the air CO2 [29]. 

The following chemical reaction allows us make 

certain calculations per the Acetone yield [29]:  

12 CO2 + 56 H2 = 3.6 Acetone + 0.06 Cell Carbon 

Again, we have to note, that our manufacturing 

facility is the grid-independent, getting all the 

electric energy solely from the solar panels 

covering all the area over our bioreactors. We 

used our original not having any World’s analogs 

gas blend fermentation vessels [1,3,5,7-18]. The 

bottom of each vessel was covered with the gas 

Pall spargers providing the size of gas blend bells 

of around 0.5 micrometers. The overall 

fermentation work went extremely well with the 

high microbial densities of the genetically 

engineered strains of Clostridium sp. MT896 and 

of Clostridium sp. MAceT113 [29]. When the 

acetone reached the concentration of about 8 - 9 

g/l in each fermentation block of our fermentation 

plant, the fermentation was deceased and the 

acetone was collected from the fermentation fluid. 

Evaporated acetone was collected and then 

subjected to the treatment under the alkaline 

catalyst per the US Patent 1550792A [4] to reach 

the production of the Diacetyl alcohol. At the 

concentration of the Diacetyl alcohol 97% it was 

collected, dehydrated from the trace amounts of 

water and used as the diesel engine fuel. So, now 

we are ready to offer to the customers the 

replacement of the diesel fuel, manufactured in the 

US solely from the air CO2.  

Materials and Methods 

In this article we describe the bioreactors used to 

manufacture our Carbon Negative fuels.  The 

image reflecting one of said bioreactors is shown 

in Fig. 5. 



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Fig.5.  Our horizontal bioreactor design [30]. 

This is the modification of the known already 

horizontal bioreactor of 

Beckman Coulter Life Sciences [11].  The idea is 

the same:  the feed is gaseous like for growing of 

Algae cells [11].  However we grow the Acetogen 

biocatalysts in one set of bioreactor modules 

producing from the gas blend of H2 and CO2 (the 

gas blend we use has 80 % vol/vol of H2 .and 20 % 

vol/vol CO2 fuel Isobutanol [7-10, 2-16,19] and in 

the other set of said bioreactor modules to 

manufacture acetone [29]. 

As Fig. 5 shows, in our system we used 14 

impellers causing random distribution of the 

gaseous blend over the bioreactor volume we have 

used.  Said gas blend was composed of 80% 

vol/vol H2 and 20% vol/vol of CO2.  This is very 

important that said CO2 was extracted from the 

surrounding air.  The process of said C02 

extraction is proprietary and we will describe it in 

our subsequent publication since we do not have 

enough money for the US patenting process and 

trust that the publications in the peer-reviewed 

Journals totally replace the related patents and 

have to be always overcome by the patents of 

competitors if any. The horizontal bioreactor 

shown in Fig. 5 has been filled by the nutrient 

medium by 67- 68%.  The rest of the bioreactor 

space was for the used by us gas blend as above.  

It is important to note that each impellers used 

were composed of the properly balanced for the 

rotation 

three polished stainless 316S steel blades at the 

speed of 450 rpm.  The pressure of  the gas blend 

used in the horizontal bioreactor shown in Fig. 5 

was 15 psi.  The total number of said diffusion 

stones was 39 per each bioreactor we have used. 

Fuel Isobutanol was manufactured using the 

genetically engineered biocatalysts per [18].  

Diacetone Alcohol (DAA)  was created from the 

Carbon Negative acetone manufactured before 

using the technology we have already described 

[29] by the condensation of the two Carbon 

Negative acetone molecules over the alkaline 

catalyst as described [29].   Said Carbon Negative 

fuel Isobutanol and DAA were used as the Carbon 

Negative fuels sold to the customers.  In case of 

fuel Isobutanol its known high viscosity causing 

problems in fuel lines of the cars at low air 

temperatures if fuel Isobutanol was used pure 

were resolved as discussed before [6] by mixing 

of 80% vol/vol of fuel Isobutanol with 20% 

vol/vol of Carbon Negative DAA as described 

herein above.  Said problem with elevated 

viscosity does not exist for the said mixture of the 



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carbon negative fuels as above:  40% of fuel 

Isobutanol, 10% fuel n-Butanol, 30%  Mesityl 

oxide [24,34,35] and 20% fuel Diacetyl alcohol 

(DAA). 

It is important to note that the impeller blades 

were placed on a 316S stainless teal shift fixed in 

the horizontal bioreactor by the Podshipniks [31]. 

These are the specially designed and properly 

manufactured for handling and rotation elements 

providing long and productive life for shift with 

the impellers shown in Fig. 4 and used in the 

bioreactor.  Podshipniks are shown in Fig. 4 and 

in Fig. 5. 

Fig. 6.  Podshipnik used in our horizontal bioreactor [30]. 

It is important to note that the Author was 

assassinated by SHELL after offering SHELL 

commercialization of his proprietary technology 

of Gasoline manufacturing from the air CO2 (the 

Declaration of Interests Statement section of this 

original article).  As the response the Author has 

came up with the proposition to displace the 

existing petroleum corporations operating in the 

USA by the corporations manufacturing Carbon 

Negative fuels from the air CO2 in sincere 

Author’s intent to bring the air CO2 content to the 

level of the year 1900 or just before the active 

petroleum fields discovery and exploration 

followed by the distillation of petroleum in the 

refineries itself producing the air CO2 [6].  

It is also important to mention herein that the 

Carbon negative fuels were sold at the new type of 

the gas stations shown in Fig 3. below. 

We have tested our Carbon Negative fuels of the 

set of corporate vehicles we have sacrificed for 

that purpose even without knowing the results of 

the testing performed but with the great hope that 

all said vehicles will survive said testing and will 

be used again but with only the Carbon Negative 

fuels. For said testing we have sacrificed corporate 

270.2020 Toyota Camry, Peugeout 3008 and the 

diesel truck 2020 Chevrolet Silverado 1500 

Duramax Diesel. The gas mileage in the town of 

Brownsville and on the highways was 

meticulously recorded by the respective 

chauffeurs Certified mechanics from the Toyota, 

Peugeot and Chevrolet dealerships were invited to 

the corporate garage of our corporations to 

meticulously check 1)stated cars driving and 2) 

any mechanical damages, especially in the engine 

compartments, like gaskets on the engines 

anticipating damage of some of them after at least 

10,000 miles of testing.  The testing was 

performed for 20,000 miles for each test vehicle.  

The records on the gas mileage for all types of 

280.tested cars are discussed herein.   

We have mentioned that we sell our Carbon 

Negative fuels at the new type of the gas stations, 

combining 1) the sales of the Carbon Negative 

fuels and 2) the sales of the certain goods for 

which the valued customers had to drive 



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additionally to Walmart and the grocery stores 

along with ordering (includes also the on-line 

ordering) of the goods the  customers could not 

find at the new type of the gas stations but needed 

and had to drive to the next to the gas stations 

store to purchase them.  In addition to that the new 

type of the gas stations was selling the bottled 

distilled water manufactured at our new type of 

business activity - the sea based set of solar panels 

powered boats for the distillation of the sea water.  

The new type of the gas stations was also used to 

sell to the valued customers - consumers of the 

Carbon Negative fuels the food for home cooking, 

not the prepared at the gas stations or ready to eat 

food sold at the old type of the gas stations.  Our 

intent was to ensure the valued customers will find 

the new type of the carbon negative gas stations a 

valuable option to save their time for not visiting  

on their cars the Walmart and the grocery stores.  

Certain foods were prepared using the genetically 

engineered fungi (to be approved for the 

Nationwide use).  We used the Ordering option 

for the goods not currently sold bu had to be 

added to save the time for our valuable customers.  

For that purpose the sales people took the orders 

and there was also the on-line ordering system. 

Development of Carbon Negative Foods. 

The genetic engineering of the fungi was 

performed as described before for the genetic 

engineering of the Acetogens-biocatalysts 

At the first step the Author has eliminated fungi 

genes not essential for the fungi maintenance and 

propagation [4]. At the second step of 308.genetic 

engineering of fungi is the cloning in fungi of the 

recombinant genes of lamb and other animals (not 

listed herein) or tomatoes, potatoes, cabbage, 

bread and spice.  Said genetically engineered 

fungi  will be cultivated in the sterile evironment 

at the corporate site. 

The special care will be taken about the 

contracting capabilities of the genetically 

engineered muscle tissues allowing the muscle 

tissues of said genetically engineered creatures to 

grow as the natural muscle tissue has to grow with 

the training of the muscle owners. 

Genetically engineered meats. 

The Author trusts that his energy and cell 

components Carbon Negative system developed 

recently for the engineered Acetogen Biocatalysts 

should work well without any complications in 

genetically engineered fungi (the species has to be 

determined yet) containing the genes of lamb 

structures as thought originally should work 

without any problems in genetically engineered 

fungi (the species has to be .determined yet) 

containing the genes of meat production.  Original 

thinking of the Author was about the lamb 

originated genes, but that might change at the 

level of operations. A variety of genes encoding 

various meat structures must be engineered to add 

the smell and the feeling at the teeth during 

chowing the exact feeling of the meat structures 

consumed.  Like the sensation of cracking the rib, 

cartilage, etc. 

The special care will be taken about the 

contracting capabilities of the genetically 

engineered muscle tissues allowing the muscle 

tissues of said genetically engineered creatures to 

grow as the natural muscle tissue has to grow with 

the training of the genetically engineered said 

333.muscle owners - genetically engineered 

muscle fibers. 

Genetically engineered tomatoes, potatoes, 

cabbage, etc. 

The Author trusts that the energy generation and 

cell components structures creation could be 

produced by the carbon negative system 

developed recently for the engineered Acetogen 

Biocatalysts [6-18] should work well without any 

complications in genetically engineered fungi (the 

species has to be determined yet) containing the 

genes of respective vegetables production.  The 

Author eventually will not be able to provide the 

exposure to the Sun light and the fresh air of said 

genetically engineered tomatoes, potatoes, 

cabbage and other vegetables which have to be 

maintained under the sterile conditions over the 

lifetime of the genetically engineered fungi-based 

systems. 

Genetically engineered bread and bread-based 

products 

The Author trusts that the energy and cell 

components Carbon Negative system developed 

recently for the engineered Acetogen Biocatalysts 

should work well without any complications in 

genetically engineered fungi (the species has to be 



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determined yet) containing the genes of respective 

bread and bread-based products production.  The 

Author has to engineer and design the system for 

baking of said genetically engineered bread and 

bread-based products.  Eventually there will be 

certain problems with not properly cooked 

genetically engineered bread and bread-based 

products. 

Genetically engineered Spices 

The Author has given great thought about spices.  

Any food especially genetically engineered one 

has to have salt and spices.  The Author did not 

think about the genetic engineering of salt, it has 

to be available with no limitations.  But 

genetically engineered spices based on pepper and 

other components used to produce spices have to 

be engineered.  The Author trusts that the energy 

and cell components Carbon Negative system 

developed recently for the engineered Acetogen 

Biocatalyst should work well without any 

complications in genetically engineered fungi (the 

species has to be determined yet) containing the 

genes of respective genetically engineered pepper 

and other components giving the taste of spices.  

The salt will be added to such genetically 

engineered spices as the powder which can be 

sterilized as usually to get into the sterile 

genetically engineered environment, or there will 

not be any need for that. 

Results 

The Author was really surprised when all his 

planned herein in this original article experimental 

parts worked well as described before [7-10, 12-

14] with the Carbon Negative replacement of

Gasoline and Diesel fuel. 

Data on testing of the carbon negative fuel using 

real Gasoline and Diesel fuels vehicles. Results on 

no any visible defects or vehicles misbehavior 

while driving 

The certified mechanics from the respective car 

dealerships meticulously tested the respective 

corporate cars at the corporate manufacturing site 

and drove said cars on the roads nearby the 

manufacturing site meticulously to reveal any 

damages caused by the replacement of the 

Gasoline or the Diesel fuel by the suggested 

mixture of fuel Diacetyl alcohol (80% v./v.) and 

Fuel Isobutanol (20% v./v.). The 40% of fuel 

Isobutanol, 10% fuel n-Butanol, 30%  Mesityl 

oxide [24,34,35] and 20% fuel Diacetyl alcohol 

(DAA) to power the Diesel truck 2022 RAM 

3500.  The cars were placed on the treadmill with 

the device to check the engine emissions. No 

unusual exhaust gas emissions were ever tested 

and discovered in the exhaust gas of all tested 

vehicles. 

No unusual emissions in the cars exhausts 

No unusual engine emissions were noted by said 

mechanics for all tested corporate cars. Then the 

mechanics made the test drives of the each said 

vehicle.  No any mechanical or any other damages 

to the engines of the all said cars were ever 

revealed upon said thorough testing.  Test drives 

of each said vehicle by the certified mechanics did 

not reveal any abnormal driving behavior in any 

of said cars.  The mechanics still did not make any 

further recommendations on the following use of 

the Gasoline and the Diesel fuel replacement 

leaving for the Author the possibility of reporting 

this very good news to the general public.  The 

gas mileages of the cars used to test the mixture of 

fuel Diacetyl alcohol (80% v./v.) and fuel 

Isobutanol (20% v./v.) or the mixture as we stated 

above40% of fuel Isobutanol, 10% fuel n-Butanol, 

30%  Mesityl oxide [24,34,35] and 20% fuel 

Diacetyl alcohol (DAA)  as the  replacement of 

Gasoline were 37 mpg on the highway and 31 

mpg in the city of Brownsville.  You can easily 

calculate the average gas mileage from said 

numbers. But the overall picture looks to us very 

promising since the gas mileage even slightly 

exceeded the Gasoline from petroleum use 

mileage in Toyota Camry and Porshe Cayenne 

Turbo.  Due to the limited number of the tested 

cars we cannot make any predictions for the other 

car makes and the cars made before the year of 

2020.  This will become the risk of the other car 

owners, who will decide to replace their gasoline 

with the produced from the air CO2 mixture of 

fuel Diacetyl alcohol (80% v./v.) and fuel 

Isobutanol (20% v./v.).  If you remember our 

corporate goal is to replace all the petroleum use 

by the use of the air CO2, initially to resolve the 

issue of the transportation problems, substantially 

increasing the air CO2 content via the air CO2 

accumulation and eventually to decrease the air 

CO2 content causing the fresh water loss to the 

outer Space vacuum as stated before.  The similar 



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picture was seen if we used said mixture 40% of 

fuel Isobutanol, 10% fuel n-Butanol, 30%  Mesityl 

oxide [24,34,35] and 20% fuel Diacetyl alcohol 

(DAA) to fuel the Diesel truck 2022 RAM 3500:  

highway mileage 37 mpg, town mileage 35 mpg 

for the long testing time of 20,000. 

Fuel efficiency of the new carbon negative 

replacement of the Gasoline and Diesel fuel 

For the use of said mixture of fuel Diacetyl 

alcohol (80% v./v.) and fuel Isobutanol (20% 

v./v.) as the Diesel fuel replacement for over the 

20,000 miles runs, the 10,000 miles old 2020 

Shevrolet Silverado1500 truck was used for test 

runs on our carbon negative Diesel fuel for 20,000 

miles.. The Diesel fuel mileage was achieved on 

the highway at the average diesel fuel mileage ~38 

mpg, and at ~32 mpg driving in the city. The 

calculated the combined driving efficiency was 

about ~36 mpg. The Author does not take any risk 

in forecasting the Diesel fuel mileage behavior of 

the other diesel cars/trucks makes and per the 

mileage forecasting for the Diesel cars/trucks 

makes older than the year of 2020. 

Discussion 

NASA has stated in 2010 after measuring the air 

CO2 content above 400 ppm that that was the 

“Point of No Return” to healthy environment on 

the Earth.  Now the air CO2 level exceeds 450 

ppm. What that means and how do we pan to 

reduce the air CO2 content to the level we have in 

our dreams - the air CO2 level of the year 1900 or 

just before the active discovery of the oil wells 

and fields and the massive petroleum production 

and use fore distillation to manufacture Gasoline 

and Diesel fuel by the international petroleum 

corporations. 

The air CO2 is the heaviest gas of the air gas blend 

having density of 1.22 g/m
3. 

 Air CO2 density

exceeds 1.97 g/m
3.  

Therefore by the gravity force

the air CO2 is covering the Ground.  The Ground 

is the natural  refuge for all kinds of fresh water:  

fresh water from rains, snow/ice melting, etc.  The 

ground is the place where from the creeks, rivers 

start from to go to the sea, lakes and ponds.  With 

the air CO2 excessively covering the Ground it 

produces much more fresh water vapors as that 

was in the year of 1900.  In the Southern States of 

the US the temperature of the Ground reaches 100 
o 

C and up.  The fresh water trapped in the Ground 

might even boil increasing the evaporation of the 

fresh water vapors to the air.  The air is attached to 

the Ground by the gravity force.  The average 

thickness of the air layer above the ground is only 

400 miles. The other end of said air layer is the 

outer Space vacuum.  In 2010 NASA has found a 

lot of ice on the dark surface of the Moon.  NASA 

has concluded that ice was captured by the dark 

surface of the Moon (-293
o
C) and the fresh water

vapors came form Earth.  The Moon worked as 

the “cold trap” for said vapors.  Compare 

diameters of earth and the Moon, thinking that the 

Moon is located in 200,000 miles distance from 

Earth.  

What that means?  Earth is wasting its fresh water 

to the outer Space.   How to stop it?  This might 

be stopped by the stopping the air CO2 production.  

For that purpose we have developed our Carbon 

Negative fuel and food corporations and 

manufacture the Carbon Negative fuels. Therefore 

we are offering the Carbon Negative fuels instead 

of the widely used Gasoline and Diesel fuel 

manufactured by the international petroleum 

corporations from petroleum on the over 80 in the 

continental US refineries and sold to customers 

Nationwide. 

The described for the Acetogen biocatalysts new 

type of the horizontal bioreactor design has the 

three gas blend diffusion stones per the perimeter 

of the bioreactor to ensure the even distribution of 

the feeding Acetogen biocatalysts  with the gas 

blend components.  The total number of said 

diffusion stones was per each bioreactor.  They 

are located evenly with three inches apart from 

each other solely in the liquid part of the 

horizontal bioreactor.  Fig. 1 has the only one of 

said diffusion stones, but the horizontal bioreactor 

for the manufacture of the Carbon Negative fuels 

has three at the perimeter of the immersed to the 

medium part three inches apart from each other 

with the total number of said stones Thirty Nine 

[30]. 

The Author suggests his own Carbon Negative 

fuels sold at the new type of gas stations shown in 

Fig. 7. 



CMI 05 (02), 600−618 
CMI JOURNAL 610 

CMI JOURNAL  Dr. Michael Vladislavovich Tyurin 

Fig. 7.  New type of the Carbon Negative fuels gas station offered herein. 

The new gas stations  (Fig. 7) selling Carbon 

Negative DAA and the Carbon Negative mixture 

as we noted above: 40% of fuel Isobutanol, 10% 

fuel n-Butanol, 30%  Mesityl oxide [24,34,35] and 

20% fuel Diacetyl alcohol (DAA) [15-17, 29]  

along with the genetically engineered foods and 

normal foods for home cooking of the customers 

showed pretty much promising tendencies of the 

improved sales of both the carbon negative fuel 

and the foods  for the home cooking and 

consumption.  Additionally said gas stations will 

sell to the customers the chemicals widely used by 

the household - and this all just to save the 

valuable time for the valuable customers. 

Apparently customers really appreciated the 

opportunity to save their time while visiting said 

new version of the gas stations selling also foods 

for the households.  Saving of the customers time 

was our primary priority and the new route to 

improve sales of the carbon negative fuels.   

Basically we have reached our goal; the new kind 

of the gas stations will probably replace the 

existing gas station selling only snacks and 

Gasoline and Diesel fuel manufactured from 

petroleum.  

Actually, said above the new type of the gas 

stations along with the offered there Carbon 

Negative fuels is the delicate response of the 

Author to SHELL with its attempted murder of 

the Author of this original article in response to 

the Author’s offer to SHELL go start the 

manufacture of the Carbon Negative fuels. 

We are concerned about the amount of the fresh 

water reserves on Earth.  Fig. 8 shows the 

Governmental data on that [33].  Fig. 9 elaborates 

these data. 



CMI 05 (02), 600−618 
CMI JOURNAL 611 

CMI JOURNAL  Dr. Michael Vladislavovich Tyurin 

Fig. 8.  Fresh water reserves of Earth [33]. 

Fig. 9. Loss of fresh water from Earth to the Space vacuum due to the elevated air CO2 concentrations 

[6,21]. 



CMI 05 (02), 600−618 
CMI JOURNAL 612 

CMI JOURNAL  Dr. Michael Vladislavovich Tyurin 

The Author came to Houston in 1998.  He was 

pleased by the Houston warm weather and always 

blue bottomless sky.  Just in passed from that 26 

years the Houston weather has changed.  There is 

rain almost every day now.  Rain is good as more 

fresh water comes to the Ground.  However we 

need to realize that the Earth atmosphere which is 

only 400 miles thick above the Ground and ends 

up with the outer Space vacuum sucking the fresh 

water vapors from Earth’s atmosphere if the air 

CO2 exceeds certain levels.  Said atmosphere 

structure is not even.  Rains are at the 

atmosphere’s bottom.  Above that there are 

different events and above that is the Space 

vacuum.  Therefore, the rainy now weather in 

Houston reflects the substantially increased 

amount of the fresh water vapors in the air gas 

blend blend.   Specifically, with the changes in 

Houston weather you can easily see that over 

accumulated air CO2 boils sea water in the 

Mexican Gulf.  Most part of Mexican Gulf is in 

the Equator area where the Sun gives for free to us 

up to 1,330 Wt/m
2
 of the solar panels.  The same

happens with the air CO2 which selectively 

absorbs the solar light infra red energy converting 

it to heat.  That heat boils sea water on the surface 

of Mexican Gulf causing changes in Houston 

weather.  South winds bring evaporated from 

Mexican Gulf fresh water condensed in the clouds 

often offering rain these days.  Only 25 years from 

the Author’s arrival to Houston and such a 

dramatic weather change.  So, you can easily see 

that the changes to the fresh water evaporation to 

the Space vacuum are coming as we now project - 

in the next 10-25 years from now. Fresh water 

shortage will mean the shortage of the crops and 

the livestock production. The ecological crisis is 

coming and it will be the worst in the history of 

the humankind.  The Author plans to prevent that 

from happening.  Fig. 10 explains the thoughts of 

the Author. 

Fig. 10.  The intelligent response of the Author to 

the attempt of SHELL to kill him after his visit 

and proposition to SHELL to commercialize the 

proprietary technology of Gasoline manufacture 

from the air CO2 for just $0.35 per gallon of 

product while SHELL and other international 

petroleum corporations spend over $1.75 to 

manufacture gallon of Gasoline or Diesel fuel 

from petroleum.  Replacement of petroleum 

corporations by the Carbon Negative corporations 

offering Carbon Negative fuels and Carbon 

Negative genetically engineered foods for the 

humankind consumption during the time of the 

proposed fresh water shortage. 



CMI 05 (02), 600−618 
CMI JOURNAL 613 

CMI JOURNAL  Dr. Michael Vladislavovich Tyurin 

The planned price for the Gasoline and Diesel fuel 

replacements is planned at the level of $2.65-

$2.87 per gallon regardless if the fuel will be used 

for the gasoline or Diesel fuel powered cars / 

trucks.  We have not had a chance to investigate 

the suitability of this new Carbon Negative 

mixture use for powering of the air jet turbines yet 

[36].  We are confident it will work even there, 

but we have to check it thoroughly and 

meticulously there like we did with the Gasoline 

or Diesel fuel powered cars in this article. 

Therefore we are gong to save the Earth’s fresh 

water escaping to the outer Space.  What fresh 

water shortage mean?  No crops and no livestock 

production.  Global starvation is coming to Earth.  

And we are going to revert it by noted herein 

means.  The Author claims he will save this 

world, he will save the Earth’s fresh water. 

Declaration of Interests Statement 

Ethics approval and consent to participate.  

The Author has received all the proper documents 

granting the Ethical Approval and the Consent to 

Participate from the State of Texas officials. The 

Author has made sure that the ethical approval and 

his consent to participate in preparation and 

submission for publication of this article were 

properly approved by the respective authorities of 

the State of Texas. 

Consent for publication.  

The Author has expressed his complete consent to 

participate in work with this article and its 

publication in this Journal. 

Availability of data and materials.  

The Author makes all his data and materials 

herein available for any third party. The data and 

materials might be obtained from the Author at 

PO Box 300230, Houston, TX, 77340. Email 

drmtyurin64@gmail.com.  If any third party needs 

any materials used to publish this article, please, 

do contact the Author. 

Competing interests. 

This article is another step in the war which has 

started by SHELL International Petroleum 

Corporation some time back by attempted murder 

of the Author.  The people hired by SHELL has 

totaled the Author’s corporate car and did some 

other illegal things to the Author which have 

concluded the Author that the war with the 

international petroleum corporations has started 

already.  No Houston FBI or Houston Police 

investigation of said attempted murder has 

happened and this is behind the responsibility of 

the Texas Governor with all the legal remedies the 

Author might use in his fight to sue the State and 

its Governor Gregory Abbott.  The attempted 

murder has the statute of limitations 20 years, and 

there are jurisdictions above the level of the State 

of TEXAS.  The Author had certain legal 

problems with the at that time Attorney General of 

the State of Texas in 2013, now Texas Governor, 

Greg Abbott and the Author is going to resolve all 

the legal problems with the Texas Governor, 

regardless would he be dismissed or not. The 

corporate Author’s website 

http://syngasbiofuelsenergy.com was destroyed by 

the person, the Attorney of Hirsch and 

Westheimer Law Firm, PC by the last name Levy. 

She belongs to the same family which owned or 

owns the grocery stores chain named  “Fiesta” in 

Houston Texas.  The Author was unable to find a 

lawyer in Texas to file the respective federal law 

suit and recover his corporate website, but the 

Author has the US Constitutional right to recite 

this website in the references herein.  Therefore 

the Author has multiple legal problems with the 

State of Texas and the Author is going to resolve 

said legal problems at the respective Court level in 

the US.  The Author was already involved into the 

war which has been started by the International 

Petroleum Corporation SHELL. The Author has 

approached SHELL offering them for the 

commercialization the proprietary technology of 

Gasoline or Diesel fuel manufacture from the air 

CO2, not from petroleum which SHELL and other 

international petroleum corporations use.  SHELL 

representative met the Author, got his draft of the 

at that time in publication article on creation 

Gasoline from the air CO2 [2]. SHELL hired two 

Mexicans to kill the Author in the car accident, 

paying the possibly around $6,000 for this dirty 

job, which has been done in Houston TEXAS at 

the US59 down South (9494 Southwest Fwy).  

The Houston FBI and the Houston Police were 

contacted by the Author multiple times over his 

mobile phone however there was no any detailed 

and thorough Police or the FBI investigation of 

said attempted murder of the Author since as the 

http://syngasbiofuelsenergy.com/


CMI 04 (03), 396−414 
CMI JOURNAL 614 

CMI JOURNAL  Dr. Michael Vladislavovich Tyurin 

 

Author trusts, both Houston FBI and Houston 

Police are totally corrupted by the international 

petroleum corporations.  Therefore any law is not 

used in the State of Texas to hurt said 

corporations.  Therefore the Author has to look for 

the law enforcement outside of the State of Texas, 

at the National level and he will do that to affect 

the Texas Governor Abbott for his actions of 2013 

and have him resign from his position. Therefore 

the Author has conflict of interests with the State 

of Texas, TEXAS Governor Gregg Abbott, Texas 

FBI and Texas Police. Since that time more 

scientific publications by the Author on the 

Gasoline and Diesel fuel production from the air 

CO2 came out [11-47].  The US patents are 

extremely expensive, therefore the Author uses 

instead of patents scientific publications.  

Publications are as good as the US patents, each 

the US patent has to claim something better than 

the Author already did in his scientific 

publications.  The Author working on the 

Acetogens-biocatalysts has no any competition in 

the world because of his prior invention, the 

electroporation / electrofusion Generator, already 

sold as a sample (with no right for reproduction) 

to the US corporation BTX, Inc. / Genetronics, 

Inc. (San-Diego, CA).  Said Generator and the 

invented by the Authorgenome tailoring 

technology make him with no any competition in 

the whole World.  The Author has conflict of 

interest with his Ph. D. Supervisor, Professor 

Boris A. Shenderov, who apparently worked for 

the KGB in the former Soviet Union.  In 1992, 

when the Author has applied form the first 

International Passport of the USSR, we went to 

the local Police department and set in line much 

before the International Passport department 

started working .  When the Author has entered 

said local Police Department he immediately was 

arrested and jailed in the local prison without any 

announcement of his fault why he was arrested.  

The Author has recently changed his job leaving 

Professor Shenderov and joining the USSR 

Research Institute GNIIGENETICA.  Therfore 

Prof. Shenderov started making every possible 

efforts to harm the Author with the use of KGB.  

Therefore the Author instead of getting his first 

International Passport of the USSR was arrested 

and without any notification of the crime he has 

committed he was arrested and forwarded with the 

machine guns armed Police workers to the local 

jail.  In the local jail the Author was placed with 

another five persons, criminals, who knew what 

for they were arrested and jailed  and smoked 

tobacco heavily right in the prison jail cell with 

the size of about 5.5 x 5.5 x 5.5 meters (about 10 x 

10 x 10 feet).  Because of said tobacco smoke the 

Author became very sick and in five hours after 

being jailed he asked the God he never prayed to 

(the Author is the ateist) to take the Author to 

Haven.  The Author wanted to die at said moment.  

His blood pressure and heart pulse were 

astonishingly high, and the Author was really sick 

at said time.  He started knocking inside the jail 

cell to the cell entrance door trying to get attention 

of the Policemen on duty.  But that was not 

effective and no medical help was rendered by the 

Police to the Author at the Author’s requests. He 

asked the God to make him dead, he asked God to 

take him to Haven.  Suddenly the Author has 

heard the God’s voice right in the Author’s head.  

The God said in English:  “This is not your time to 

die yet .  You have not done your major business 

yet.  Calm down, try to relax and wait till the 

Police understands that they were mistaken with 

you and will release you”.  Indeed in a few days 

the Author was released and has received his first 

International passport of the USSR.  In 2001 the 

Author has recovered that he has being doing 

wrong things at his work and started working on 

the Carbon Negative fuels and genetically 

engineered foods on the base of Acetogens.  That 

was the major business the Russian God told to 

the Author in 1992 at the jail cell when the Author 

has asked his God to take him to Haven and die.  

Therefore the Author has the conflict of interest 

with his former Ph. D.  Supervisor Prof.  B. A. 

Shenderov. The Author states herein that certain 

articles  in the reference section cited Berzin V.  

Berzin VG was the business partner of the Author 

during his time at Syngas Biofuels Energy. Inc 

(2007-2013).  He even paid his own $300 to start 

said business and spoke to his personal attorney to 

start said business registering it with the state of 

TX. However Berzin V did not participate in no 

actual work at said corporation and obviously did 

not do any contributions to the articles listed in the 

reference section herein.  His authorship was 

included as such as he paid for said business.  

That is all the readers want to know.  Berzin V. 

retired at the age of 65 and went to the retirement 

house which name is kept by him strictly 



CMI 05 (02), 600−618 
CMI JOURNAL 615 

CMI JOURNAL  Dr. Michael Vladislavovich Tyurin 

confidential to avoid the Author of contacting him 

at any time. The Author recites publications where 

the authors were Drs. M. Kiriukhin and E. Gak.  

Said authors were paid significant amounts of 

cash by the Author for doing their research when 

the Author has hired them in the Russian 

Federation to do said R & D projects on the 

Acetogens when the Author was the CEO, 

Executive Chairman, President of Tyurin 

Enterprises, LLC. 

Funding.  

The Author has received no funding. The 

investors of the Author has read this article draft 

to his investors and they have decided not to 

include no funding information as they have stated 

to the Author. They stated the Author should write 

“No Funding” per this original article. No funding.  

No funding was used to prepare and publish this 

original article.  The available investors have 

declined to provide their information for this 

publication.  They have suggested to note that the 

article was prepared and published with no any 

funding source. 

Authors' contributions.  

The Author has conducted all the experiments 

himself.    The Author has planned, wrote this 

original article and edited the written text, 

including proper placement of the illustrations 

mentioned.  The Author read, edited and approved 

the final manuscript. The Author is the only owner 

of all materials disclosed in this original article. 

The Author has plans to distribute his proprietary 

products after their approval as needed.  The 

Author might be contacted for the data and 

materials at PO  Box  300230, Houston, TX, 

77230,  drmtyurin64@gmail.com.  The Author 

contributed to the study conception and design. 

Material preparation, data collection and analysis 

were performed by the Author. The first draft of 

the manuscript was written by the Author. The 

Author read and approved the final manuscript. 

Acknowledgements.  

The Author does have the other parties to 

acknowledge. Ms. Joany Kerr, the International 

Sales Director of BTX, Inc./ Genetronics, Inc.,  

and the CEO of  BTX, Inc./ Genetronics, Inc. Dr. 

Gunter A. Hoffman for giving to the Author his 

copy of the Electrofusion / Electrotransformation 

Generator prior to that sold to BTX, Inc./ 

Genetronics, Inc. The Author confirms herein the 

help of the Third Secretary of the US Embassy in 

Moscow at that time (1995) Mr. James 

Winkelman for his help to receive funds for the 

transportation of said Generator from Moscow to 

San-Diego via UPS in 1995. The Author herein 

confirms help of Dr. Nikita Tamm from the 

Moscow State University for his help in receiving 

cash in $ in Moscow, the Russian Federation, 

from San-Diego, CA  in 1995. 

Authors' information. 

The Author  has the B. Sci. In Biological 

Engineering (1984), M. Sci. In Biology (1985), 

M. D. in Internal Medicine (1986) (all from 

Saratov State Medical University, the USSR) in 

addition to the Ph. D. in Molecular Biology, 

Molecular Pharmacology, Microbiology (1990) 

from the USSR Research Institute for Antibiotics 

(Moscow, the USSR).  The Author Dr. Tyurin 

Michael Vladislavovich has changed his second 

name from “Vladimirovich” to “Vladislavovich” 

after his father, Professor, Dr.  Tyurin Vladimir 

Iljich has changed his first name from “Vladimir” 

to “Vladislav” in 2017. The Author at his 

corporations can be reached at PO Box 300230 .in 

Houston TX 77230 and via Email 

drmtyurin64gmail.com. 

Originality-Significance Statement 

The Author has wrote this original article based on 

his originality of the business approach and the 

existing resistance of the International Petroleum 

corporation to the technology of manufacturing 

carbon negative fuels to replace their production 

of fuels originating from petroleum.  The 

reduction of the air CO2 levels towards the pre-

petroleum era of the year 1900 is paramount,since 

our planet looses fresh water to the outer Space 

vacuum.  NASA has confirmed that in 2010 sating 

that the Earth has reached the “Point of No 

Return” to the healthy environmental conditions 

suitable for life on our planet.  The new family of 

the gas stations selling not only Carbon Negative 

fuels but also foods for cooking at home and 

commodity chemicals for the households will save 

a lot of time for the customers of said new gas 

stations. 



CMI 05 (02), 600−618 
CMI JOURNAL 616 

CMI JOURNAL  Dr. Michael Vladislavovich Tyurin 

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Genome tailoring powered production of

isobutanol in continuous CO2/H2 blend

fermentation using engineered acetogen

biocatalyst. J Ind Microbiol Biotechnol.

41(5):763-781.

2. Morrison RT, Boyd RN (1992). Organic

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3. Tyurin M. 2013. Gene replacement and

elimination using λRed- and FLP-based tool to 

re-direct carbon flux in acetogen biocatalyst 

during continuous CO₂/H₂ blend fermentation. 

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Alcohol:: 9786204749587: Amazon.com: 

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https://pubhtml5.com/pobs/%20twbs/Diacetone_Alcohol_as_the_Diesel_Fuel/16
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CMI 05 (02), 600−618 
CMI JOURNAL 617 

CMI JOURNAL  Dr. Michael Vladislavovich Tyurin 

and electrofusion/electrodistruction of various 

cells: microbial, animal, plant and the process 

of its manufacture, and the set of plate-parallel 

polished electrodes and the process of their 

manufacture from the titanium-based metal 

alloy VK-2.  

20. Tyurin MV (1992). Russian Patent RU 2-

005776-2. Plate-parallel polished electrodes

and the process of their manufacture from the

titanium-based metal alloy VK-2.

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Genome tailoring powered production of

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fermentation using engineered acetogen

biocatalyst. J Ind Microbiol Biotechnol.

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lox66/lox71-based elimination of

phosphotransacetylase or acetaldehyde

dehydrogenase shifted carbon flux in acetogen

rendering selective overproduction of ethanol

or acetate. Appl Biochem Biotechnol.

195(3):181-8. https://link.springer.com/article

/10.1007/s12010-012-9864-8.

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html.

35. US Patent 1232897 Preparation of Isophorone

and Mesityl oxide from Acetone. Filed 1984-

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CMI 05 (02), 600−618 CMI JOURNAL 618 

CMI JOURNAL  Dr. Michael Vladislavovich Tyurin 

36. US Patent 3,413,372.   Process for the

synthesis of mesitylene.  Filed 1966-09-29.

Expired 1985-11-26 (Lifetime).

37. Jet Fuel: Biofuels are slowly coming to

business jets - AOPA.

https://www.aopa.org/news-and-media/all-news/2020/february/pilot/turbine-pilot-biofuel
https://www.aopa.org/news-and-media/all-news/2020/february/pilot/turbine-pilot-biofuel



