id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
ct-10183	Ferrer, Maria S. ; Palomares, Roberto A. ; Bullington, Anna-Claire ; Jabur Bittar, João Henrique 	Effect of liposome-containing diluent and centrifugation on motion parameters and membrane integrity of electroejaculated cooled bovine spermatozoa	2018	10	.pdf	application/pdf	4907	273	43	Crespilho AM, Papa FO, Santos MP, et al: Use of cooled bull semen as a strategy to increase the pregnancy rate in fixed-time artificial insemination programs-case report. Keywords: Cryopreservation, bovine, centrifugation, motility, chilled semen Introduction The use of cooled shipped semen for artificial insemination is a routine procedure in the equine and canine industries.1,2 Maintaining semen at refrigeration temperature lowers the metabolic rate of spermatozoa and decreases overgrowth of bacteria, extending the lifespan of spermatozoa.3 In general, pregnancy rates achieved with cooled semen are higher than with frozen semen.4,5 This is because freezing and thawing induce sperm damage associated with plasma membrane reorganization, accumulation of intracellular ions, osmotic stress, cryoprotectant toxicity and formation of intracellular ice crystals, altering sperm viability and function.6,7	cache/ct-10183.pdf	txt/ct-10183.txt