id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
ct-12690	Filho, Roberto Rosa ; Vieira, Nívea ; Brito, Maíra ; Losano, João ; Kawai, Giulia ; Mendes, Camilla ; Rui, Bruno; Nagai, Ken ; Alves, Álvaro ; Frias, Henrique ; Sousa, Raphaela ; Sanches, Fábio ; Assumpção, Mayra ; Nichi, Marcilio 	Effects of nonenzymatic and enzymatic antioxidants in cryopreserved canine semen extended with docosahexaenoic acid	2025	10	.pdf	application/pdf	6192	260	44	Effect of vitamin E and catalase and their combination to sperm oxidative stress features http://dx.doi.org/10.58292/CT.v17.12690 https://doi.org/10.1016/j.theriogenology.2007.04.053 https://doi.org/10.1016/S0304-4157(00)00018-6 https://doi.org/10.1155/2014/190542 https://doi.org/10.1530/rep.0.1210315 https://doi.org/10.1530/rep.0.1210315 http://www.cbra.org.br/pages/publicacoes/rbra/v34n2/p79-86.pdf http://www.cbra.org.br/pages/publicacoes/rbra/v34n2/p79-86.pdf 8 Citation: Clinical Theriogenology 2025, 17, 12690, http://dx.doi.org/10.58292/CT.v17.12690 6. In addition, lipid composition of sperm membrane in several species influences resistance to cold shock during refrigeration and cryopreservation.6 Therefore, polyunsaturated fatty acids are also directly involved in protecting the membrane during cryopreserva- tion.7,8 An alternative would be to supplement the samples with PUFAs during cryopreservation, protecting sperm against cryoinjury.9 However, the high percentage of PUFAs makes sperm susceptible to oxidizing agents, leading to lipid peroxi- dation (due to their large amount of unsaturation, they are more easily oxidized).5 Additionally, for canine semen preser- vation, it is necessary to remove seminal plasma, and with that important antioxidants are removed.10 When reactive oxy- gen species (ROS) act on the unsaturated fatty acids’ double bonds, a lipid peroxidation chain reaction begins and, if not interrupted, leads to loss of membrane permeability and, con- sequently, sperm function.11 Based on these assertions, sperm *Cofirst authors mailto:mnichi@usp.br http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ http://dx.doi.org/10.58292/CT.v17.12690 2 Citation: Clinical Theriogenology 2025, 17, 12690, http://dx.doi.org/10.58292/CT.v17.12690 treatment with PUFAs during cryopreservation could provide ambiguous results, but if PUFAs are incorporated into the sperm membrane, this would make it even more susceptible to ROS attack.	cache/ct-12690.pdf	txt/ct-12690.txt