id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
ct-9454	Bothun, Alisha ; Woods, Dori 	Steps toward the generation of ovarian somatic cells from pluripotent stem cells	2020	7	.pdf	application/pdf	3892	160	39	and it was not until 2012 that functional oocytes capable of maturation, fertilization, and the ability to give rise to live offspring were realized.3 Notably, the protocol that was used to accomplish this feat, as well as a subsequent report by the same group, relied on the innate ability of PSC-derived primordial germ cell-like cells (PGCLCs) to interact with endogenous fetal ovarian tissue, providing the microenvironment and granulosa cells that enabled follicle growth and maturation, and with it oocyte development.3-5 An additional recent report in which functional eggs were generated from induced PSCs (iPSCs) derived from granulosa cells similarly used fetal ovarian tissue.6 Together, these reports highlighted the notion that the fetal ovarian somatic microenvironment is not only nurturing, but likely requisite for the development of functional eggs from PSC sources. Generating large numbers of granulosa cells by this method that are capable of hormone synthesis would require healthy granulosa cells to generate iPSCs, a lengthy and costly endeavor to complete on a per person basis.	cache/ct-9454.pdf	txt/ct-9454.txt