id sid tid token lemma pos ct-10670 1 1 1 1 NUM ct-10670 1 2 contact contact NOUN ct-10670 1 3 stuart stuart PROPN ct-10670 1 4 meyers meyers PROPN ct-10670 1 5 smeyers@ucdavis.edu smeyers@ucdavis.edu PROPN ct-10670 1 6 supplemental supplemental ADJ ct-10670 1 7 data datum NOUN ct-10670 1 8 for for ADP ct-10670 1 9 this this DET ct-10670 1 10 article article NOUN ct-10670 1 11 can can AUX ct-10670 1 12 be be AUX ct-10670 1 13 accessed access VERB ct-10670 1 14 here here ADV ct-10670 1 15 . . PUNCT ct-10670 2 1 © © ADP ct-10670 2 2 2024 2024 NUM ct-10670 2 3 the the DET ct-10670 2 4 author(s author(s NOUN ct-10670 2 5 ) ) PUNCT ct-10670 2 6 . . PUNCT ct-10670 3 1 this this PRON ct-10670 3 2 is be AUX ct-10670 3 3 an an DET ct-10670 3 4 open open ADJ ct-10670 3 5 access access NOUN ct-10670 3 6 article article NOUN ct-10670 3 7 distributed distribute VERB ct-10670 3 8 under under ADP ct-10670 3 9 the the DET ct-10670 3 10 terms term NOUN ct-10670 3 11 of of ADP ct-10670 3 12 the the DET ct-10670 3 13 creative creative ADJ ct-10670 3 14 commons common NOUN ct-10670 3 15 attribution attribution NOUN ct-10670 3 16 - - PUNCT ct-10670 3 17 noncommercial noncommercial ADJ ct-10670 3 18 4.0 4.0 NUM ct-10670 3 19 international international ADJ ct-10670 3 20 license license NOUN ct-10670 3 21 ( ( PUNCT ct-10670 3 22 http:// http:// PROPN ct-10670 3 23 creativecommons.org/licenses/by-nc/4.0/ creativecommons.org/licenses/by-nc/4.0/ NOUN ct-10670 3 24 ) ) PUNCT ct-10670 3 25 , , PUNCT ct-10670 3 26 permitting permit VERB ct-10670 3 27 all all DET ct-10670 3 28 noncommercial noncommercial ADJ ct-10670 3 29 use use NOUN ct-10670 3 30 , , PUNCT ct-10670 3 31 distribution distribution NOUN ct-10670 3 32 , , PUNCT ct-10670 3 33 and and CCONJ ct-10670 3 34 reproduction reproduction NOUN ct-10670 3 35 in in ADP ct-10670 3 36 any any DET ct-10670 3 37 medium medium NOUN ct-10670 3 38 , , PUNCT ct-10670 3 39 provided provide VERB ct-10670 3 40 the the DET ct-10670 3 41 original original ADJ ct-10670 3 42 work work NOUN ct-10670 3 43 is be AUX ct-10670 3 44 properly properly ADV ct-10670 3 45 cited cite VERB ct-10670 3 46 . . PUNCT ct-10670 4 1 citation citation NOUN ct-10670 4 2 : : PUNCT ct-10670 4 3 clinical clinical ADJ ct-10670 4 4 theriogenology theriogenology NOUN ct-10670 4 5 2024 2024 NUM ct-10670 4 6 , , PUNCT ct-10670 4 7 16 16 NUM ct-10670 4 8 , , PUNCT ct-10670 4 9 10670 10670 NUM ct-10670 4 10 , , PUNCT ct-10670 4 11 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PRON ct-10670 4 12 review review AUX ct-10670 4 13 report report VERB ct-10670 4 14 intracytoplasmic intracytoplasmic ADJ ct-10670 4 15 sperm sperm NOUN ct-10670 4 16 injection injection NOUN ct-10670 4 17 zone zone NOUN ct-10670 4 18 : : PUNCT ct-10670 4 19 insights insight NOUN ct-10670 4 20 and and CCONJ ct-10670 4 21 applications application NOUN ct-10670 4 22 from from ADP ct-10670 4 23 a a DET ct-10670 4 24 university university NOUN ct-10670 4 25 - - PUNCT ct-10670 4 26 based base VERB ct-10670 4 27 assisted assist VERB ct-10670 4 28 reproduction reproduction NOUN ct-10670 4 29 laboratory laboratory PROPN ct-10670 4 30 katarzyna katarzyna PROPN ct-10670 4 31 malin malin PROPN ct-10670 4 32 , , PUNCT ct-10670 4 33 a a DET ct-10670 4 34 alejandro alejandro PROPN ct-10670 4 35 de de PROPN ct-10670 4 36 la la PROPN ct-10670 4 37 fuente fuente PROPN ct-10670 4 38 , , PUNCT ct-10670 4 39 a a DET ct-10670 4 40 , , PUNCT ct-10670 4 41 b b PROPN ct-10670 4 42 thadeu thadeu NOUN ct-10670 4 43 de de X ct-10670 4 44 castro castro PROPN ct-10670 4 45 , , PUNCT ct-10670 4 46 a a DET ct-10670 4 47 soledad soledad PROPN ct-10670 4 48 martin martin PROPN ct-10670 4 49 - - PUNCT ct-10670 4 50 pelaez pelaez PROPN ct-10670 4 51 , , PUNCT ct-10670 4 52 a a DET ct-10670 4 53 margo margo NOUN ct-10670 4 54     SPACE ct-10670 4 55 verstraete verstraete NOUN ct-10670 4 56 , , PUNCT ct-10670 4 57 a a DET ct-10670 4 58 pouya pouya ADJ ct-10670 4 59 dini dini NOUN ct-10670 4 60 , , PUNCT ct-10670 4 61 a a DET ct-10670 4 62 stuart stuart PROPN ct-10670 4 63 meyersb meyersb PROPN ct-10670 4 64 * * PROPN ct-10670 4 65 adepartments adepartment NOUN ct-10670 4 66 of of ADP ct-10670 4 67 population population NOUN ct-10670 4 68 health health NOUN ct-10670 4 69 and and CCONJ ct-10670 4 70 reproduction reproduction NOUN ct-10670 4 71 , , PUNCT ct-10670 4 72 banatomy banatomy NOUN ct-10670 4 73 , , PUNCT ct-10670 4 74 physiology physiology NOUN ct-10670 4 75 , , PUNCT ct-10670 4 76 and and CCONJ ct-10670 4 77 cell cell NOUN ct-10670 4 78 biology biology NOUN ct-10670 4 79 , , PUNCT ct-10670 4 80 school school NOUN ct-10670 4 81     SPACE ct-10670 4 82 of of ADP ct-10670 4 83     SPACE ct-10670 4 84 veterinary veterinary ADJ ct-10670 4 85     SPACE ct-10670 4 86 medicine medicine NOUN ct-10670 4 87 , , PUNCT ct-10670 4 88 university university PROPN ct-10670 4 89 of of ADP ct-10670 4 90 california california PROPN ct-10670 4 91 , , PUNCT ct-10670 4 92 davis davis PROPN ct-10670 4 93 , , PUNCT ct-10670 4 94 ca ca PROPN ct-10670 4 95 , , PUNCT ct-10670 4 96 usa usa PROPN ct-10670 4 97 abstract abstract ADJ ct-10670 4 98 intracytoplasmic intracytoplasmic ADJ ct-10670 4 99 sperm sperm NOUN ct-10670 4 100 injection injection NOUN ct-10670 4 101 ( ( PUNCT ct-10670 4 102 icsi icsi NOUN ct-10670 4 103 ) ) PUNCT ct-10670 4 104 has have AUX ct-10670 4 105 become become VERB ct-10670 4 106 one one NUM ct-10670 4 107 of of ADP ct-10670 4 108 the the DET ct-10670 4 109 most most ADV ct-10670 4 110 important important ADJ ct-10670 4 111 tools tool NOUN ct-10670 4 112 used use VERB ct-10670 4 113 for for ADP ct-10670 4 114 in in ADP ct-10670 4 115 vitro vitro X ct-10670 4 116 production production NOUN ct-10670 4 117 of of ADP ct-10670 4 118 embryos embryo NOUN ct-10670 4 119 ( ( PUNCT ct-10670 4 120 ivp ivp NOUN ct-10670 4 121 ) ) PUNCT ct-10670 4 122 in in ADP ct-10670 4 123 equine equine NOUN ct-10670 4 124 reproduction reproduction NOUN ct-10670 4 125 management management NOUN ct-10670 4 126 programs program NOUN ct-10670 4 127 around around ADP ct-10670 4 128 the the DET ct-10670 4 129 world world NOUN ct-10670 4 130 . . PUNCT ct-10670 5 1 this this DET ct-10670 5 2 procedure procedure NOUN ct-10670 5 3 is be AUX ct-10670 5 4 often often ADV ct-10670 5 5 performed perform VERB ct-10670 5 6 in in ADP ct-10670 5 7 the the DET ct-10670 5 8 us us PROPN ct-10670 5 9 for for ADP ct-10670 5 10 several several ADJ ct-10670 5 11 breeds breed NOUN ct-10670 5 12 and and CCONJ ct-10670 5 13 is be AUX ct-10670 5 14 used use VERB ct-10670 5 15 primarily primarily ADV ct-10670 5 16 to to PART ct-10670 5 17 optimize optimize VERB ct-10670 5 18 foal foal NOUN ct-10670 5 19 production production NOUN ct-10670 5 20 for for ADP ct-10670 5 21 broodmares broodmare NOUN ct-10670 5 22 and and CCONJ ct-10670 5 23 performance performance NOUN ct-10670 5 24 mares mare NOUN ct-10670 5 25 but but CCONJ ct-10670 5 26 also also ADV ct-10670 5 27 for for ADP ct-10670 5 28 stallions stallion NOUN ct-10670 5 29 with with ADP ct-10670 5 30 limited limited ADJ ct-10670 5 31 semen semen NOUN ct-10670 5 32 availability availability NOUN ct-10670 5 33 or or CCONJ ct-10670 5 34 poor poor ADJ ct-10670 5 35 semen semen NOUN ct-10670 5 36 quality quality NOUN ct-10670 5 37 . . PUNCT ct-10670 6 1 as as ADP ct-10670 6 2 such such ADJ ct-10670 6 3 , , PUNCT ct-10670 6 4 there there PRON ct-10670 6 5 are be VERB ct-10670 6 6 a a DET ct-10670 6 7 limited limited ADJ ct-10670 6 8 number number NOUN ct-10670 6 9 of of ADP ct-10670 6 10 us we PRON ct-10670 6 11 laboratories laboratory NOUN ct-10670 6 12 capable capable ADJ ct-10670 6 13 of of ADP ct-10670 6 14 using use VERB ct-10670 6 15 this this DET ct-10670 6 16 method method NOUN ct-10670 6 17 , , PUNCT ct-10670 6 18 which which PRON ct-10670 6 19 requires require VERB ct-10670 6 20 advanced advanced ADJ ct-10670 6 21 training training NOUN ct-10670 6 22 of of ADP ct-10670 6 23 personnel personnel NOUN ct-10670 6 24 and and CCONJ ct-10670 6 25 specialized specialized ADJ ct-10670 6 26 equipment equipment NOUN ct-10670 6 27 . . PUNCT ct-10670 7 1 many many ADJ ct-10670 7 2 veterinarians veterinarian NOUN ct-10670 7 3 and and CCONJ ct-10670 7 4 breeding breeding NOUN ct-10670 7 5 farms farm NOUN ct-10670 7 6 currently currently ADV ct-10670 7 7 aspirate aspirate VERB ct-10670 7 8 ovarian ovarian ADJ ct-10670 7 9 follicles follicle NOUN ct-10670 7 10 in in ADP ct-10670 7 11 cycling cycling NOUN ct-10670 7 12 mares mare NOUN ct-10670 7 13 and and CCONJ ct-10670 7 14 ship ship NOUN ct-10670 7 15 oocytes oocyte NOUN ct-10670 7 16 to to ADP ct-10670 7 17 icsi icsi NOUN ct-10670 7 18 - - PUNCT ct-10670 7 19 capable capable ADJ ct-10670 7 20 laboratories laboratory NOUN ct-10670 7 21 , , PUNCT ct-10670 7 22 where where SCONJ ct-10670 7 23 embryos embryo NOUN ct-10670 7 24 can can AUX ct-10670 7 25 be be AUX ct-10670 7 26 produced produce VERB ct-10670 7 27 and and CCONJ ct-10670 7 28 typically typically ADV ct-10670 7 29 vitrified vitrify VERB ct-10670 7 30 for for ADP ct-10670 7 31 ultralow ultralow ADJ ct-10670 7 32 temperature temperature NOUN ct-10670 7 33 storage storage NOUN ct-10670 7 34 and and CCONJ ct-10670 7 35 transport transport NOUN ct-10670 7 36 for for ADP ct-10670 7 37 transfer transfer NOUN ct-10670 7 38 to to ADP ct-10670 7 39 recipient recipient NOUN ct-10670 7 40 mares mare NOUN ct-10670 7 41 and and CCONJ ct-10670 7 42 establishment establishment NOUN ct-10670 7 43 of of ADP ct-10670 7 44 surrogate surrogate ADJ ct-10670 7 45 pregnancies pregnancy NOUN ct-10670 7 46 . . PUNCT ct-10670 8 1 the the DET ct-10670 8 2 veterinary veterinary ADJ ct-10670 8 3 assisted assist VERB ct-10670 8 4 reproduction reproduction NOUN ct-10670 8 5 laboratory laboratory NOUN ct-10670 8 6 in in ADP ct-10670 8 7 the the DET ct-10670 8 8 school school NOUN ct-10670 8 9 of of ADP ct-10670 8 10 veterinary veterinary ADJ ct-10670 8 11 medicine medicine NOUN ct-10670 8 12 at at ADP ct-10670 8 13 the the DET ct-10670 8 14 university university PROPN ct-10670 8 15 of of ADP ct-10670 8 16 california california PROPN ct-10670 8 17 , , PUNCT ct-10670 8 18 davis davis PROPN ct-10670 8 19 , , PUNCT ct-10670 8 20 maintains maintain VERB ct-10670 8 21 a a DET ct-10670 8 22 3 3 NUM ct-10670 8 23 - - PUNCT ct-10670 8 24 dimensional dimensional ADJ ct-10670 8 25 approach approach NOUN ct-10670 8 26 to to ADP ct-10670 8 27 equine equine NOUN ct-10670 8 28 assisted assist VERB ct-10670 8 29 reproductive reproductive ADJ ct-10670 8 30 technology technology NOUN ct-10670 8 31 . . PUNCT ct-10670 9 1 we we PRON ct-10670 9 2 offer offer VERB ct-10670 9 3 commercial commercial ADJ ct-10670 9 4 solutions solution NOUN ct-10670 9 5 to to ADP ct-10670 9 6 breeders breeder NOUN ct-10670 9 7 , , PUNCT ct-10670 9 8 educational educational ADJ ct-10670 9 9 advice advice NOUN ct-10670 9 10 and and CCONJ ct-10670 9 11 training training NOUN ct-10670 9 12 to to ADP ct-10670 9 13 other other ADJ ct-10670 9 14 laboratories laboratory NOUN ct-10670 9 15 , , PUNCT ct-10670 9 16 veterinarians veterinarian NOUN ct-10670 9 17 , , PUNCT ct-10670 9 18 and and CCONJ ct-10670 9 19 visiting visit VERB ct-10670 9 20 scholars scholar NOUN ct-10670 9 21 . . PUNCT ct-10670 10 1 moreover moreover ADV ct-10670 10 2 , , PUNCT ct-10670 10 3 as as ADP ct-10670 10 4 a a DET ct-10670 10 5 research research NOUN ct-10670 10 6 laboratory laboratory NOUN ct-10670 10 7 , , PUNCT ct-10670 10 8 our our PRON ct-10670 10 9 objective objective NOUN ct-10670 10 10 is be AUX ct-10670 10 11 to to PART ct-10670 10 12 analyze analyze VERB ct-10670 10 13 and and CCONJ ct-10670 10 14 apply apply VERB ct-10670 10 15 observations observation NOUN ct-10670 10 16 from from ADP ct-10670 10 17 ivp ivp PRON ct-10670 10 18 to to ADP ct-10670 10 19 elucidation elucidation NOUN ct-10670 10 20 of of ADP ct-10670 10 21 complex complex ADJ ct-10670 10 22 developmental developmental ADJ ct-10670 10 23 problems problem NOUN ct-10670 10 24 such such ADJ ct-10670 10 25 as as ADP ct-10670 10 26 embryonic embryonic ADJ ct-10670 10 27 and and CCONJ ct-10670 10 28 fetal fetal ADJ ct-10670 10 29 loss loss NOUN ct-10670 10 30 . . PUNCT ct-10670 11 1 in in ADP ct-10670 11 2 2018 2018 NUM ct-10670 11 3 , , PUNCT ct-10670 11 4 we we PRON ct-10670 11 5 reported report VERB ct-10670 11 6 the the DET ct-10670 11 7 birth birth NOUN ct-10670 11 8 of of ADP ct-10670 11 9 the the DET ct-10670 11 10 first first ADJ ct-10670 11 11 foal foal NOUN ct-10670 11 12 produced produce VERB ct-10670 11 13 at at ADP ct-10670 11 14 uc uc PROPN ct-10670 11 15 davis davis PROPN ct-10670 11 16 using use VERB ct-10670 11 17 icsi icsi NOUN ct-10670 11 18 , , PUNCT ct-10670 11 19 and and CCONJ ct-10670 11 20 we we PRON ct-10670 11 21 have have AUX ct-10670 11 22 since since ADV ct-10670 11 23 developed develop VERB ct-10670 11 24 a a DET ct-10670 11 25 commercial commercial ADJ ct-10670 11 26 icsi icsi NOUN ct-10670 11 27 program program NOUN ct-10670 11 28 for for ADP ct-10670 11 29 practitioners practitioner NOUN ct-10670 11 30 and and CCONJ ct-10670 11 31 horse horse NOUN ct-10670 11 32 breeders breeder NOUN ct-10670 11 33 in in ADP ct-10670 11 34 the the DET ct-10670 11 35 us us PROPN ct-10670 11 36 . . PUNCT ct-10670 12 1 today today NOUN ct-10670 12 2 , , PUNCT ct-10670 12 3 our our PRON ct-10670 12 4 laboratory laboratory NOUN ct-10670 12 5 receives receive VERB ct-10670 12 6 thousands thousand NOUN ct-10670 12 7 of of ADP ct-10670 12 8 immature immature ADJ ct-10670 12 9 oocytes oocyte NOUN ct-10670 12 10 for for ADP ct-10670 12 11 icsi icsi NOUN ct-10670 12 12 sessions session NOUN ct-10670 12 13 every every DET ct-10670 12 14 year year NOUN ct-10670 12 15 , , PUNCT ct-10670 12 16 with with ADP ct-10670 12 17 an an DET ct-10670 12 18 average average NOUN ct-10670 12 19 of of ADP ct-10670 12 20 2 2 NUM ct-10670 12 21 embryos embryo NOUN ct-10670 12 22 per per ADP ct-10670 12 23 mare mare NOUN ct-10670 12 24 - - PUNCT ct-10670 12 25 session session NOUN ct-10670 12 26 . . PUNCT ct-10670 13 1 our our PRON ct-10670 13 2 research research NOUN ct-10670 13 3 is be AUX ct-10670 13 4 focused focus VERB ct-10670 13 5 on on ADP ct-10670 13 6 molecular molecular ADJ ct-10670 13 7 , , PUNCT ct-10670 13 8 cellular cellular ADJ ct-10670 13 9 and and CCONJ ct-10670 13 10 genetic genetic ADJ ct-10670 13 11 aspects aspect NOUN ct-10670 13 12 of of ADP ct-10670 13 13 gamete gamete ADJ ct-10670 13 14 biology biology NOUN ct-10670 13 15 , , PUNCT ct-10670 13 16 perifertilization perifertilization NOUN ct-10670 13 17 events event NOUN ct-10670 13 18 , , PUNCT ct-10670 13 19 and and CCONJ ct-10670 13 20 early early ADJ ct-10670 13 21 equine equine NOUN ct-10670 13 22 development development NOUN ct-10670 13 23 using use VERB ct-10670 13 24 an an DET ct-10670 13 25 array array NOUN ct-10670 13 26 of of ADP ct-10670 13 27 tools tool NOUN ct-10670 13 28 including include VERB ct-10670 13 29 advanced advanced ADJ ct-10670 13 30 microscopy microscopy NOUN ct-10670 13 31 , , PUNCT ct-10670 13 32 sequencing sequencing NOUN ct-10670 13 33 , , PUNCT ct-10670 13 34 and and CCONJ ct-10670 13 35 time time NOUN ct-10670 13 36 - - PUNCT ct-10670 13 37 lapse lapse NOUN ct-10670 13 38 imaging imaging NOUN ct-10670 13 39 of of ADP ct-10670 13 40 developing develop VERB ct-10670 13 41 embryos embryo NOUN ct-10670 13 42 . . PUNCT ct-10670 14 1 in in ADP ct-10670 14 2 this this DET ct-10670 14 3 review review NOUN ct-10670 14 4 , , PUNCT ct-10670 14 5 we we PRON ct-10670 14 6 have have AUX ct-10670 14 7 highlighted highlight VERB ct-10670 14 8 our our PRON ct-10670 14 9 laboratory laboratory NOUN ct-10670 14 10 ’s ’s PART ct-10670 14 11 current current ADJ ct-10670 14 12 methods method NOUN ct-10670 14 13 for for ADP ct-10670 14 14 commercial commercial ADJ ct-10670 14 15 equine equine NOUN ct-10670 14 16 ivp ivp NOUN ct-10670 14 17 and and CCONJ ct-10670 14 18 research research NOUN ct-10670 14 19 and and CCONJ ct-10670 14 20 clinical clinical ADJ ct-10670 14 21 studies study NOUN ct-10670 14 22 conducted conduct VERB ct-10670 14 23 to to PART ct-10670 14 24 optimize optimize VERB ct-10670 14 25 ivp ivp NOUN ct-10670 14 26 in in ADP ct-10670 14 27 horses horse NOUN ct-10670 14 28 . . PUNCT ct-10670 15 1 keywords keyword NOUN ct-10670 15 2 : : PUNCT ct-10670 15 3 equine equine NOUN ct-10670 15 4 embryo embryo NOUN ct-10670 15 5 , , PUNCT ct-10670 15 6 intracytoplasmic intracytoplasmic ADJ ct-10670 15 7 sperm sperm NOUN ct-10670 15 8 injection injection NOUN ct-10670 15 9 , , PUNCT ct-10670 15 10 noninvasive noninvasive ADJ ct-10670 15 11 time time NOUN ct-10670 15 12 - - PUNCT ct-10670 15 13 lapse lapse NOUN ct-10670 15 14 microscopy microscopy NOUN ct-10670 15 15 introduction1 introduction1 ADJ ct-10670 15 16 in in ADP ct-10670 15 17 the the DET ct-10670 15 18 late late ADJ ct-10670 15 19 nineteenth nineteenth ADJ ct-10670 15 20 century century NOUN ct-10670 15 21 , , PUNCT ct-10670 15 22 experiments experiment NOUN ct-10670 15 23 in in ADP ct-10670 15 24 artificial artificial ADJ ct-10670 15 25 insemination insemination NOUN ct-10670 15 26 in in ADP ct-10670 15 27 animals animal NOUN ct-10670 15 28 were be AUX ct-10670 15 29 conducted conduct VERB ct-10670 15 30 in in ADP ct-10670 15 31 multiple multiple ADJ ct-10670 15 32 research research NOUN ct-10670 15 33 centers center NOUN ct-10670 15 34 across across ADP ct-10670 15 35 europe europe PROPN ct-10670 15 36 . . PUNCT ct-10670 16 1 the the DET ct-10670 16 2 pioneering pioneering ADJ ct-10670 16 3 work work NOUN ct-10670 16 4 of of ADP ct-10670 16 5 ilya ilya PROPN ct-10670 16 6 ivanov ivanov PROPN ct-10670 16 7 and and CCONJ ct-10670 16 8 walter walter PROPN ct-10670 16 9 heape heape PROPN ct-10670 16 10 inspired inspire VERB ct-10670 16 11 an an DET ct-10670 16 12 emerging emerge VERB ct-10670 16 13 generation generation NOUN ct-10670 16 14 of of ADP ct-10670 16 15 scientists scientist NOUN ct-10670 16 16 to to PART ct-10670 16 17 further far ADV ct-10670 16 18 develop develop VERB ct-10670 16 19 this this PRON ct-10670 16 20 and and CCONJ ct-10670 16 21 other other ADJ ct-10670 16 22 gamete gamete NOUN ct-10670 16 23 techniques technique NOUN ct-10670 16 24 , , PUNCT ct-10670 16 25 with with ADP ct-10670 16 26 the the DET ct-10670 16 27 first first ADJ ct-10670 16 28 american american ADJ ct-10670 16 29 artificial artificial ADJ ct-10670 16 30 insemination insemination NOUN ct-10670 16 31 ( ( PUNCT ct-10670 16 32 ai ai NOUN ct-10670 16 33 ) ) PUNCT ct-10670 16 34 cooperative cooperative NOUN ct-10670 16 35 in in ADP ct-10670 16 36 cattle cattle NOUN ct-10670 16 37 * * NOUN ct-10670 16 38 recipient recipient NOUN ct-10670 16 39 of of ADP ct-10670 16 40 2023 2023 NUM ct-10670 16 41 bartlett bartlett PROPN ct-10670 16 42 award award PROPN ct-10670 16 43 , , PUNCT ct-10670 16 44 editor editor NOUN ct-10670 16 45 appreciates appreciate VERB ct-10670 16 46 author author NOUN ct-10670 16 47 ’s ’s PART ct-10670 16 48 willingness willingness NOUN ct-10670 16 49 to to PART ct-10670 16 50 prepare prepare VERB ct-10670 16 51 and and CCONJ ct-10670 16 52 submit submit VERB ct-10670 16 53 a a DET ct-10670 16 54 manuscript manuscript NOUN ct-10670 16 55 for for ADP ct-10670 16 56 peer peer NOUN ct-10670 16 57 review review NOUN ct-10670 16 58 ; ; PUNCT ct-10670 16 59 author author PROPN ct-10670 16 60 ’s ’s PART ct-10670 16 61 bartlett bartlett PROPN ct-10670 16 62 address address NOUN ct-10670 16 63 is be AUX ct-10670 16 64 posted post VERB ct-10670 16 65 in in ADP ct-10670 16 66 the the DET ct-10670 16 67 following follow VERB ct-10670 16 68 journal journal PROPN ct-10670 16 69 url url PROPN ct-10670 16 70 . . PUNCT ct-10670 17 1 https://doi.org/10.58292/ https://doi.org/10.58292/ PROPN ct-10670 17 2 ct.v15.9776 ct.v15.9776 PROPN ct-10670 17 3 established establish VERB ct-10670 17 4 in in ADP ct-10670 17 5 1938.1 1938.1 NUM ct-10670 17 6 it it PRON ct-10670 17 7 took take VERB ct-10670 17 8 almost almost ADV ct-10670 17 9 a a PRON ct-10670 17 10 century century NOUN ct-10670 17 11 to to PART ct-10670 17 12 commercialize commercialize VERB ct-10670 17 13     SPACE ct-10670 17 14 embryo embryo NOUN ct-10670 17 15 transfer transfer NOUN ct-10670 17 16 and and CCONJ ct-10670 17 17 embryo embryo NOUN ct-10670 17 18 transportation transportation NOUN ct-10670 17 19 ; ; PUNCT ct-10670 17 20 embryo embryo NOUN ct-10670 17 21 vitrification vitrification NOUN ct-10670 17 22 was be AUX ct-10670 17 23 introduced introduce VERB ct-10670 17 24 only only ADV ct-10670 17 25 in in ADP ct-10670 17 26 1996.2,3 1996.2,3 NUM ct-10670 17 27 today today NOUN ct-10670 17 28 , , PUNCT ct-10670 17 29 advanced advanced ADJ ct-10670 17 30 technological technological ADJ ct-10670 17 31 solutions solution NOUN ct-10670 17 32 such such ADJ ct-10670 17 33 as as ADP ct-10670 17 34 ivp ivp PROPN ct-10670 17 35 , , PUNCT ct-10670 17 36 biopsy biopsy NOUN ct-10670 17 37 for for ADP ct-10670 17 38 preimplantation preimplantation NOUN ct-10670 17 39 genetic genetic ADJ ct-10670 17 40 diagnosis diagnosis NOUN ct-10670 17 41 , , PUNCT ct-10670 17 42 embryo embryo NOUN ct-10670 17 43 vitrification vitrification NOUN ct-10670 17 44 , , PUNCT ct-10670 17 45 and and CCONJ ct-10670 17 46 time time NOUN ct-10670 17 47 - - PUNCT ct-10670 17 48 lapse lapse NOUN ct-10670 17 49 microscopy microscopy NOUN ct-10670 17 50 have have AUX ct-10670 17 51 become become VERB ct-10670 17 52 routine routine ADJ ct-10670 17 53 procedures procedure NOUN ct-10670 17 54 for for ADP ct-10670 17 55 equine equine NOUN ct-10670 17 56 embryologists embryologist NOUN ct-10670 17 57     SPACE ct-10670 17 58 in in ADP ct-10670 17 59 their their PRON ct-10670 17 60 endeavors endeavor NOUN ct-10670 17 61 to to PART ct-10670 17 62 optimize optimize VERB ct-10670 17 63 pregnancy pregnancy NOUN ct-10670 17 64 outcomes outcome NOUN ct-10670 17 65 and and CCONJ ct-10670 17 66 further far ADV ct-10670 17 67 understand understand VERB ct-10670 17 68 equine equine NOUN ct-10670 17 69 development development NOUN ct-10670 17 70 , , PUNCT ct-10670 17 71 with with ADP ct-10670 17 72 the the DET ct-10670 17 73 potential potential NOUN ct-10670 17 74 for for ADP ct-10670 17 75 involvement involvement NOUN ct-10670 17 76 of of ADP ct-10670 17 77 artificial artificial ADJ ct-10670 17 78 intelligence intelligence NOUN ct-10670 17 79 in in ADP ct-10670 17 80 the the DET ct-10670 17 81 near near PROPN ct-10670 17 82 future.4 future.4 PROPN ct-10670 17 83 intracytoplasmic intracytoplasmic ADJ ct-10670 17 84 sperm sperm NOUN ct-10670 17 85 injection injection NOUN ct-10670 17 86 ( ( PUNCT ct-10670 17 87 icsi icsi NOUN ct-10670 17 88 ) ) PUNCT ct-10670 17 89 was be AUX ct-10670 17 90 initially initially ADV ct-10670 17 91 developed develop VERB ct-10670 17 92 in in ADP ct-10670 17 93 19925 19925 NUM ct-10670 17 94 as as ADP ct-10670 17 95 a a DET ct-10670 17 96 natural natural ADJ ct-10670 17 97 development development NOUN ct-10670 17 98 of of ADP ct-10670 17 99 subzonal subzonal ADJ ct-10670 17 100 mailto:smeyers@ucdavis.edu mailto:smeyers@ucdavis.edu NOUN ct-10670 17 101 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 X ct-10670 17 102 http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ PROPN ct-10670 17 103 http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ PROPN ct-10670 17 104 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PROPN ct-10670 17 105 https://doi.org/10.58292/ct.v15.9776 https://doi.org/10.58292/ct.v15.9776 VERB ct-10670 17 106 https://doi.org/10.58292/ct.v15.9776 https://doi.org/10.58292/ct.v15.9776 NOUN ct-10670 17 107 2 2 NUM ct-10670 17 108 citation citation NOUN ct-10670 17 109 : : PUNCT ct-10670 17 110 clinical clinical ADJ ct-10670 17 111 theriogenology theriogenology NOUN ct-10670 17 112 2024 2024 NUM ct-10670 17 113 , , PUNCT ct-10670 17 114 16 16 NUM ct-10670 17 115 , , PUNCT ct-10670 17 116 10670 10670 NUM ct-10670 17 117 , , PUNCT ct-10670 17 118 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PROPN ct-10670 17 119 insemination.6 insemination.6 VERB ct-10670 17 120 the the DET ct-10670 17 121 resulting result VERB ct-10670 17 122 manuscript manuscript NOUN ct-10670 17 123 was be AUX ct-10670 17 124 published publish VERB ct-10670 17 125 in in ADP ct-10670 17 126 the the DET ct-10670 17 127 medical medical ADJ ct-10670 17 128 journal journal PROPN ct-10670 17 129 lancet lancet PROPN ct-10670 17 130 , , PUNCT ct-10670 17 131 which which PRON ct-10670 17 132 highlighted highlight VERB ct-10670 17 133 the the DET ct-10670 17 134 worldwide worldwide ADJ ct-10670 17 135 impact impact NOUN ct-10670 17 136 of of ADP ct-10670 17 137 advanced advanced ADJ ct-10670 17 138 reproductive reproductive ADJ ct-10670 17 139 technologies technology NOUN ct-10670 17 140 . . PUNCT ct-10670 18 1 as as ADP ct-10670 18 2 of of ADP ct-10670 18 3 2024 2024 NUM ct-10670 18 4 , , PUNCT ct-10670 18 5 icsi icsi NOUN ct-10670 18 6 remains remain VERB ct-10670 18 7 the the DET ct-10670 18 8 only only ADV ct-10670 18 9 commercially commercially ADV ct-10670 18 10 reliable reliable ADJ ct-10670 18 11 method method NOUN ct-10670 18 12 for for ADP ct-10670 18 13 ivp ivp NOUN ct-10670 18 14 in in ADP ct-10670 18 15 horses.7 horses.7 NOUN ct-10670 18 16 - - PUNCT ct-10670 18 17 10 10 NUM ct-10670 18 18 in in ADP ct-10670 18 19 this this DET ct-10670 18 20 species specie NOUN ct-10670 18 21 , , PUNCT ct-10670 18 22 several several ADJ ct-10670 18 23 laboratories laboratory NOUN ct-10670 18 24 in in ADP ct-10670 18 25 the the DET ct-10670 18 26 us us PROPN ct-10670 18 27 and and CCONJ ct-10670 18 28 europe europe PROPN ct-10670 18 29 routinely routinely ADV ct-10670 18 30 report report VERB ct-10670 18 31 ~ ~ PUNCT ct-10670 18 32 20 20 NUM ct-10670 18 33 - - SYM ct-10670 18 34 25 25 NUM ct-10670 18 35 % % NOUN ct-10670 18 36 success success NOUN ct-10670 18 37 rate rate NOUN ct-10670 18 38 in in ADP ct-10670 18 39 blastocyst blastocyst NOUN ct-10670 18 40 production production NOUN ct-10670 18 41 with with ADP ct-10670 18 42 an an DET ct-10670 18 43 average average NOUN ct-10670 18 44 of of ADP ct-10670 18 45 ~ ~ PUNCT ct-10670 18 46 2 2 NUM ct-10670 18 47 blastocysts blastocyst NOUN ct-10670 18 48 per per ADP ct-10670 18 49 successful successful ADJ ct-10670 18 50 icsi icsi NOUN ct-10670 18 51 session session NOUN ct-10670 18 52 for for ADP ct-10670 18 53 most most ADJ ct-10670 18 54 mares.11 mares.11 NOUN ct-10670 18 55 - - PUNCT ct-10670 18 56 13 13 NUM ct-10670 18 57 however however ADV ct-10670 18 58 , , PUNCT ct-10670 18 59 there there PRON ct-10670 18 60 has have AUX ct-10670 18 61 been be AUX ct-10670 18 62 at at ADP ct-10670 18 63 least least ADJ ct-10670 18 64 one one NUM ct-10670 18 65 recent recent ADJ ct-10670 18 66 report report NOUN ct-10670 18 67 regarding regard VERB ct-10670 18 68 successful successful ADJ ct-10670 18 69 conventional conventional ADJ ct-10670 18 70 in in ADP ct-10670 18 71 vitro vitro X ct-10670 18 72 fertilization fertilization NOUN ct-10670 18 73 ( ( PUNCT ct-10670 18 74 ivf ivf NOUN ct-10670 18 75 ) ) PUNCT ct-10670 18 76 in in ADP ct-10670 18 77 horses horse NOUN ct-10670 18 78 with with ADP ct-10670 18 79 viable viable ADJ ct-10670 18 80 offspring offspring NOUN ct-10670 18 81 obtained obtain VERB ct-10670 18 82 in in ADP ct-10670 18 83 a a DET ct-10670 18 84 research research NOUN ct-10670 18 85 setting.14 setting.14 NOUN ct-10670 18 86 the the DET ct-10670 18 87 veterinary veterinary ADJ ct-10670 18 88 assisted assist VERB ct-10670 18 89 reproduction reproduction NOUN ct-10670 18 90 laboratory laboratory NOUN ct-10670 18 91 in in ADP ct-10670 18 92 the the DET ct-10670 18 93 school school NOUN ct-10670 18 94 of of ADP ct-10670 18 95 veterinary veterinary ADJ ct-10670 18 96 medicine medicine NOUN ct-10670 18 97 at at ADP ct-10670 18 98 the the DET ct-10670 18 99 university university PROPN ct-10670 18 100 of of ADP ct-10670 18 101 california california PROPN ct-10670 18 102 , , PUNCT ct-10670 18 103 davis davis PROPN ct-10670 18 104 maintains maintain VERB ct-10670 18 105 a a DET ct-10670 18 106 3 3 NUM ct-10670 18 107 - - PUNCT ct-10670 18 108 dimensional dimensional ADJ ct-10670 18 109 approach approach NOUN ct-10670 18 110 to to ADP ct-10670 18 111 equine equine NOUN ct-10670 18 112 assisted assist VERB ct-10670 18 113 reproductive reproductive ADJ ct-10670 18 114 technology technology NOUN ct-10670 18 115 . . PUNCT ct-10670 19 1 first first ADV ct-10670 19 2 , , PUNCT ct-10670 19 3 as as ADP ct-10670 19 4 a a DET ct-10670 19 5 clinical clinical ADJ ct-10670 19 6 service service NOUN ct-10670 19 7 , , PUNCT ct-10670 19 8 we we PRON ct-10670 19 9 offer offer VERB ct-10670 19 10 reproductive reproductive ADJ ct-10670 19 11 solutions solution NOUN ct-10670 19 12 to to PART ct-10670 19 13 horse horse NOUN ct-10670 19 14 breeders breeder NOUN ct-10670 19 15 and and CCONJ ct-10670 19 16 veterinarians veterinarian NOUN ct-10670 19 17 ( ( PUNCT ct-10670 19 18 transvaginal transvaginal ADJ ct-10670 19 19 follicular follicular ADJ ct-10670 19 20 aspiration aspiration NOUN ct-10670 19 21 , , PUNCT ct-10670 19 22 oocyte oocyte NOUN ct-10670 19 23 in in ADP ct-10670 19 24 vitro vitro X ct-10670 19 25 maturation maturation NOUN ct-10670 19 26 , , PUNCT ct-10670 19 27 postmortem postmortem NOUN ct-10670 19 28 gamete gamete NOUN ct-10670 19 29 retrieval retrieval NOUN ct-10670 19 30 , , PUNCT ct-10670 19 31 in in ADP ct-10670 19 32 vitro vitro X ct-10670 19 33 embryo embryo NOUN ct-10670 19 34 production production NOUN ct-10670 19 35 , , PUNCT ct-10670 19 36 embryo embryo NOUN ct-10670 19 37 biopsy biopsy NOUN ct-10670 19 38 and and CCONJ ct-10670 19 39 vitrification vitrification NOUN ct-10670 19 40 , , PUNCT ct-10670 19 41 intracytoplasmic intracytoplasmic ADJ ct-10670 19 42 sperm sperm NOUN ct-10670 19 43 injection injection NOUN ct-10670 19 44 , , PUNCT ct-10670 19 45 cell cell NOUN ct-10670 19 46 isolation isolation NOUN ct-10670 19 47 and and CCONJ ct-10670 19 48 storage storage NOUN ct-10670 19 49 for for ADP ct-10670 19 50 posterior posterior ADJ ct-10670 19 51 cloning cloning NOUN ct-10670 19 52 , , PUNCT ct-10670 19 53 and and CCONJ ct-10670 19 54 embryo embryo NOUN ct-10670 19 55 transfer transfer NOUN ct-10670 19 56 services service NOUN ct-10670 19 57 ) ) PUNCT ct-10670 19 58 . . PUNCT ct-10670 20 1 secondly secondly ADV ct-10670 20 2 , , PUNCT ct-10670 20 3 as as ADP ct-10670 20 4 an an DET ct-10670 20 5 academic academic ADJ ct-10670 20 6 institution institution NOUN ct-10670 20 7 , , PUNCT ct-10670 20 8 we we PRON ct-10670 20 9 provide provide VERB ct-10670 20 10 educational educational ADJ ct-10670 20 11 support support NOUN ct-10670 20 12 and and CCONJ ct-10670 20 13 training training NOUN ct-10670 20 14 to to ADP ct-10670 20 15 veterinarians veterinarian NOUN ct-10670 20 16 and and CCONJ ct-10670 20 17 visiting visit VERB ct-10670 20 18 scholars scholar NOUN ct-10670 20 19 . . PUNCT ct-10670 21 1 finally finally ADV ct-10670 21 2 , , PUNCT ct-10670 21 3 as as ADP ct-10670 21 4 a a DET ct-10670 21 5 research research NOUN ct-10670 21 6 facility facility NOUN ct-10670 21 7 , , PUNCT ct-10670 21 8 we we PRON ct-10670 21 9 aim aim VERB ct-10670 21 10 to to PART ct-10670 21 11 apply apply VERB ct-10670 21 12 conclusions conclusion NOUN ct-10670 21 13 and and CCONJ ct-10670 21 14 findings finding NOUN ct-10670 21 15 from from ADP ct-10670 21 16 in in ADP ct-10670 21 17 vitro vitro X ct-10670 21 18 production production NOUN ct-10670 21 19 of of ADP ct-10670 21 20 embryos embryo NOUN ct-10670 21 21 to to ADP ct-10670 21 22 the the DET ct-10670 21 23 elucidation elucidation NOUN ct-10670 21 24 of of ADP ct-10670 21 25 complex complex ADJ ct-10670 21 26 developmental developmental ADJ ct-10670 21 27 problems problem NOUN ct-10670 21 28 such such ADJ ct-10670 21 29 as as ADP ct-10670 21 30 embryonic embryonic ADJ ct-10670 21 31 and and CCONJ ct-10670 21 32 fetal fetal ADJ ct-10670 21 33 loss loss NOUN ct-10670 21 34 . . PUNCT ct-10670 22 1 in in ADP ct-10670 22 2 the the DET ct-10670 22 3 last last ADJ ct-10670 22 4 ~ ~ PUNCT ct-10670 22 5 5 5 NUM ct-10670 22 6 years year NOUN ct-10670 22 7 , , PUNCT ct-10670 22 8 our our PRON ct-10670 22 9 laboratory laboratory NOUN ct-10670 22 10 has have AUX ct-10670 22 11 placed place VERB ct-10670 22 12 special special ADJ ct-10670 22 13 emphasis emphasis NOUN ct-10670 22 14 on on ADP ct-10670 22 15 the the DET ct-10670 22 16 study study NOUN ct-10670 22 17 of of ADP ct-10670 22 18 embryo embryo NOUN ct-10670 22 19 morphokinetics morphokinetic NOUN ct-10670 22 20 using use VERB ct-10670 22 21 noninvasive noninvasive ADJ ct-10670 22 22 imaging imaging NOUN ct-10670 22 23 incubation incubation NOUN ct-10670 22 24 technology technology NOUN ct-10670 22 25 , , PUNCT ct-10670 22 26 which which PRON ct-10670 22 27 allowed allow VERB ct-10670 22 28 us we PRON ct-10670 22 29 to to PART ct-10670 22 30 monitor monitor VERB ct-10670 22 31 embryo embryo NOUN ct-10670 22 32 development development NOUN ct-10670 22 33 in in ADP ct-10670 22 34 near near ADP ct-10670 22 35 real real ADJ ct-10670 22 36 - - PUNCT ct-10670 22 37 time time NOUN ct-10670 22 38 without without ADP ct-10670 22 39 removing remove VERB ct-10670 22 40 embryos embryo NOUN ct-10670 22 41 from from ADP ct-10670 22 42 the the DET ct-10670 22 43 incubator incubator NOUN ct-10670 22 44 to to PART ct-10670 22 45 visualize visualize VERB ct-10670 22 46 them.13 them.13 PROPN ct-10670 22 47 this this DET ct-10670 22 48 innovation innovation NOUN ct-10670 22 49 provides provide VERB ct-10670 22 50 insight insight NOUN ct-10670 22 51 into into ADP ct-10670 22 52 not not PART ct-10670 22 53 only only ADV ct-10670 22 54 fundamental fundamental ADJ ct-10670 22 55 equine equine NOUN ct-10670 22 56 biology biology NOUN ct-10670 22 57 , , PUNCT ct-10670 22 58 which which PRON ct-10670 22 59 is be AUX ct-10670 22 60 applicable applicable ADJ ct-10670 22 61 to to ADP ct-10670 22 62 other other ADJ ct-10670 22 63 species specie NOUN ct-10670 22 64 , , PUNCT ct-10670 22 65 but but CCONJ ct-10670 22 66 also also ADV ct-10670 22 67 allows allow VERB ct-10670 22 68 us we PRON ct-10670 22 69 to to PART ct-10670 22 70 optimize optimize VERB ct-10670 22 71 equine equine NOUN ct-10670 22 72 embryo embryo NOUN ct-10670 22 73 production production NOUN ct-10670 22 74 , , PUNCT ct-10670 22 75 serving serve VERB ct-10670 22 76 the the DET ct-10670 22 77 horse horse NOUN ct-10670 22 78 industry industry NOUN ct-10670 22 79 directly directly ADV ct-10670 22 80 . . PUNCT ct-10670 23 1 in in ADP ct-10670 23 2 each each DET ct-10670 23 3 icsi icsi ADJ ct-10670 23 4 session session NOUN ct-10670 23 5 , , PUNCT ct-10670 23 6 time time NOUN ct-10670 23 7 - - PUNCT ct-10670 23 8 lapse lapse NOUN ct-10670 23 9 imaging imaging NOUN ct-10670 23 10 provides provide VERB ct-10670 23 11 an an DET ct-10670 23 12 insight insight NOUN ct-10670 23 13 into into ADP ct-10670 23 14 the the DET ct-10670 23 15 development development NOUN ct-10670 23 16 from from ADP ct-10670 23 17 zygote zygote NUM ct-10670 23 18 to to ADP ct-10670 23 19 blastocyst blastocyst NOUN ct-10670 23 20 stage stage NOUN ct-10670 23 21 , , PUNCT ct-10670 23 22 when when SCONJ ct-10670 23 23 the the DET ct-10670 23 24 embryo embryo NOUN ct-10670 23 25 is be AUX ct-10670 23 26 transferable transferable ADJ ct-10670 23 27 into into ADP ct-10670 23 28 a a DET ct-10670 23 29 recipient recipient NOUN ct-10670 23 30 mare mare NOUN ct-10670 23 31 , , PUNCT ct-10670 23 32 and and CCONJ ct-10670 23 33 predict predict VERB ct-10670 23 34 which which PRON ct-10670 23 35 embryos embryo NOUN ct-10670 23 36 are be AUX ct-10670 23 37 most most ADV ct-10670 23 38 likely likely ADJ ct-10670 23 39 to to PART ct-10670 23 40 develop develop VERB ct-10670 23 41 to to ADP ct-10670 23 42 a a DET ct-10670 23 43 healthy healthy ADJ ct-10670 23 44 foal foal NOUN ct-10670 23 45 . . PUNCT ct-10670 24 1 we we PRON ct-10670 24 2 have have AUX ct-10670 24 3 also also ADV ct-10670 24 4 been be AUX ct-10670 24 5 focusing focus VERB ct-10670 24 6 on on ADP ct-10670 24 7 advanced advanced ADJ ct-10670 24 8 microscopy microscopy NOUN ct-10670 24 9 techniques technique NOUN ct-10670 24 10 , , PUNCT ct-10670 24 11 such such ADJ ct-10670 24 12 as as ADP ct-10670 24 13 conventional conventional ADJ ct-10670 24 14 and and CCONJ ct-10670 24 15 laser laser NOUN ct-10670 24 16 - - PUNCT ct-10670 24 17 scanning scan VERB ct-10670 24 18 confocal confocal ADJ ct-10670 24 19 immunofluorescence immunofluorescence NOUN ct-10670 24 20 as as ADV ct-10670 24 21 well well ADV ct-10670 24 22 as as ADP ct-10670 24 23 genomic genomic NOUN ct-10670 24 24 and and CCONJ ct-10670 24 25 transcriptomic transcriptomic ADJ ct-10670 24 26 approaches approach NOUN ct-10670 24 27 to to ADP ct-10670 24 28 gametes gamete NOUN ct-10670 24 29 , , PUNCT ct-10670 24 30 embryos embryo NOUN ct-10670 24 31 , , PUNCT ct-10670 24 32 and and CCONJ ct-10670 24 33 single single ADJ ct-10670 24 34 - - PUNCT ct-10670 24 35 cell cell NOUN ct-10670 24 36 dynamics dynamic NOUN ct-10670 24 37 . . PUNCT ct-10670 25 1 in in ADP ct-10670 25 2 2018 2018 NUM ct-10670 25 3 , , PUNCT ct-10670 25 4 we we PRON ct-10670 25 5 reported report VERB ct-10670 25 6 the the DET ct-10670 25 7 birth birth NOUN ct-10670 25 8 of of ADP ct-10670 25 9 the the DET ct-10670 25 10 first first ADJ ct-10670 25 11 foal foal NOUN ct-10670 25 12 produced produce VERB ct-10670 25 13 at at ADP ct-10670 25 14 uc uc PROPN ct-10670 25 15 davis davis PROPN ct-10670 25 16 using use VERB ct-10670 25 17 icsi icsi NOUN ct-10670 25 18 , , PUNCT ct-10670 25 19 and and CCONJ ct-10670 25 20 we we PRON ct-10670 25 21 have have AUX ct-10670 25 22 since since SCONJ ct-10670 25 23 focused focus VERB ct-10670 25 24 on on ADP ct-10670 25 25 developing develop VERB ct-10670 25 26 a a DET ct-10670 25 27 commercial commercial ADJ ct-10670 25 28 icsi icsi NOUN ct-10670 25 29 program program NOUN ct-10670 25 30 for for ADP ct-10670 25 31 horse horse NOUN ct-10670 25 32 breeders breeder NOUN ct-10670 25 33 in in ADP ct-10670 25 34 the the DET ct-10670 25 35 us us PROPN ct-10670 25 36 . . PUNCT ct-10670 26 1 today today NOUN ct-10670 26 2 , , PUNCT ct-10670 26 3 our our PRON ct-10670 26 4 laboratory laboratory NOUN ct-10670 26 5 performs perform VERB ct-10670 26 6 hundreds hundred NOUN ct-10670 26 7 of of ADP ct-10670 26 8 icsi icsi ADJ ct-10670 26 9 sessions session NOUN ct-10670 26 10 every every DET ct-10670 26 11 year year NOUN ct-10670 26 12 , , PUNCT ct-10670 26 13 with with ADP ct-10670 26 14 an an DET ct-10670 26 15 average average NOUN ct-10670 26 16 of of ADP ct-10670 26 17 2 2 NUM ct-10670 26 18 embryos embryo NOUN ct-10670 26 19 per per ADP ct-10670 26 20 mare mare NOUN ct-10670 26 21 - - PUNCT ct-10670 26 22 session session NOUN ct-10670 26 23 . . PUNCT ct-10670 27 1 an an DET ct-10670 27 2 essential essential ADJ ct-10670 27 3 value value NOUN ct-10670 27 4 of of ADP ct-10670 27 5 icsi icsi NOUN ct-10670 27 6 is be AUX ct-10670 27 7 that that SCONJ ct-10670 27 8 the the DET ct-10670 27 9 shipment shipment NOUN ct-10670 27 10 of of ADP ct-10670 27 11 oocytes oocyte NOUN ct-10670 27 12 and and CCONJ ct-10670 27 13 sperm sperm NOUN ct-10670 27 14 does do AUX ct-10670 27 15 not not PART ct-10670 27 16 require require VERB ct-10670 27 17 transportation transportation NOUN ct-10670 27 18 of of ADP ct-10670 27 19 the the DET ct-10670 27 20 animal animal NOUN ct-10670 27 21 , , PUNCT ct-10670 27 22 and and CCONJ ct-10670 27 23 valuable valuable ADJ ct-10670 27 24 mares mare NOUN ct-10670 27 25 and and CCONJ ct-10670 27 26 stallions stallion NOUN ct-10670 27 27 are be AUX ct-10670 27 28 not not PART ct-10670 27 29 subjected subject VERB ct-10670 27 30 to to ADP ct-10670 27 31 the the DET ct-10670 27 32 risks risk NOUN ct-10670 27 33 and and CCONJ ct-10670 27 34 costs cost NOUN ct-10670 27 35 associated associate VERB ct-10670 27 36 with with ADP ct-10670 27 37 transportation transportation NOUN ct-10670 27 38 . . PUNCT ct-10670 28 1 moreover moreover ADV ct-10670 28 2 , , PUNCT ct-10670 28 3 multiple multiple ADJ ct-10670 28 4 embryos embryo NOUN ct-10670 28 5 can can AUX ct-10670 28 6 be be AUX ct-10670 28 7 obtained obtain VERB ct-10670 28 8 in in ADP ct-10670 28 9 a a DET ct-10670 28 10 single single ADJ ct-10670 28 11 icsi icsi NOUN ct-10670 28 12 session session NOUN ct-10670 28 13 and and CCONJ ct-10670 28 14 transferred transfer VERB ct-10670 28 15 into into ADP ct-10670 28 16 recipients recipient NOUN ct-10670 28 17 located locate VERB ct-10670 28 18 in in ADP ct-10670 28 19 different different ADJ ct-10670 28 20 parts part NOUN ct-10670 28 21 of of ADP ct-10670 28 22 the the DET ct-10670 28 23 country country NOUN ct-10670 28 24 . . PUNCT ct-10670 29 1 the the DET ct-10670 29 2 demand demand NOUN ct-10670 29 3 for for ADP ct-10670 29 4 this this DET ct-10670 29 5 service service NOUN ct-10670 29 6 in in ADP ct-10670 29 7 the the DET ct-10670 29 8 us us PROPN ct-10670 29 9 and and CCONJ ct-10670 29 10 abroad abroad ADV ct-10670 29 11 is be AUX ct-10670 29 12 continuing continue VERB ct-10670 29 13 to to PART ct-10670 29 14 increase increase VERB ct-10670 29 15 . . PUNCT ct-10670 30 1 in in ADP ct-10670 30 2 this this DET ct-10670 30 3 review review NOUN ct-10670 30 4 , , PUNCT ct-10670 30 5 we we PRON ct-10670 30 6 discuss discuss VERB ct-10670 30 7 our our PRON ct-10670 30 8 practical practical ADJ ct-10670 30 9 approach approach NOUN ct-10670 30 10 to to ADP ct-10670 30 11 icsi icsi NOUN ct-10670 30 12 , , PUNCT ct-10670 30 13 lessons lesson NOUN ct-10670 30 14 learned learn VERB ct-10670 30 15 , , PUNCT ct-10670 30 16 and and CCONJ ct-10670 30 17 potential potential ADJ ct-10670 30 18 applications application NOUN ct-10670 30 19 of of ADP ct-10670 30 20 this this DET ct-10670 30 21 reproductive reproductive ADJ ct-10670 30 22 technology technology NOUN ct-10670 30 23 . . PUNCT ct-10670 31 1 laboratory laboratory NOUN ct-10670 31 2 protocols protocol NOUN ct-10670 31 3 , , PUNCT ct-10670 31 4 general general ADJ ct-10670 31 5 commentary commentary NOUN ct-10670 31 6 embryologists embryologist NOUN ct-10670 31 7 in in ADP ct-10670 31 8 our our PRON ct-10670 31 9 laboratory laboratory NOUN ct-10670 31 10 are be AUX ct-10670 31 11 responsible responsible ADJ ct-10670 31 12 for for ADP ct-10670 31 13 all all DET ct-10670 31 14 media medium NOUN ct-10670 31 15 preparation preparation NOUN ct-10670 31 16 , , PUNCT ct-10670 31 17 which which PRON ct-10670 31 18 is be AUX ct-10670 31 19 performed perform VERB ct-10670 31 20 in in ADP ct-10670 31 21 a a DET ct-10670 31 22 laminar laminar ADJ ct-10670 31 23 - - PUNCT ct-10670 31 24 flow flow NOUN ct-10670 31 25 biosafety biosafety NOUN ct-10670 31 26 cabinet cabinet NOUN ct-10670 31 27 using use VERB ct-10670 31 28 reported report VERB ct-10670 31 29 preparations preparation NOUN ct-10670 31 30 for for ADP ct-10670 31 31 maturation maturation NOUN ct-10670 31 32 medium medium NOUN ct-10670 31 33 ( ( PUNCT ct-10670 31 34 discussed discuss VERB ct-10670 31 35 below).11,15 below).11,15 NOUN ct-10670 31 36 since since SCONJ ct-10670 31 37 our our PRON ct-10670 31 38 main main ADJ ct-10670 31 39 interest interest NOUN ct-10670 31 40 is be AUX ct-10670 31 41 optimizing optimize VERB ct-10670 31 42 the the DET ct-10670 31 43 use use NOUN ct-10670 31 44 of of ADP ct-10670 31 45 gametes gamete NOUN ct-10670 31 46 and and CCONJ ct-10670 31 47 ultimately ultimately ADV ct-10670 31 48 producing produce VERB ct-10670 31 49 more more ADJ ct-10670 31 50 embryos embryo NOUN ct-10670 31 51 , , PUNCT ct-10670 31 52 it it PRON ct-10670 31 53 is be AUX ct-10670 31 54 necessary necessary ADJ ct-10670 31 55 to to PART ct-10670 31 56 maintain maintain VERB ct-10670 31 57 the the DET ct-10670 31 58 highest high ADJ ct-10670 31 59 standard standard NOUN ct-10670 31 60 of of ADP ct-10670 31 61 hygiene hygiene NOUN ct-10670 31 62 as as SCONJ ct-10670 31 63 to to PART ct-10670 31 64 not not PART ct-10670 31 65 pose pose VERB ct-10670 31 66 a a DET ct-10670 31 67 threat threat NOUN ct-10670 31 68 to to ADP ct-10670 31 69 gamete gamete VERB ct-10670 31 70 , , PUNCT ct-10670 31 71 zygote zygote ADJ ct-10670 31 72 , , PUNCT ct-10670 31 73 or or CCONJ ct-10670 31 74 embryo embryo NOUN ct-10670 31 75 health health NOUN ct-10670 31 76 . . PUNCT ct-10670 32 1 positive positive ADJ ct-10670 32 2 pressure pressure NOUN ct-10670 32 3 ventilation ventilation NOUN ct-10670 32 4 in in ADP ct-10670 32 5 the the DET ct-10670 32 6 icsi icsi ADJ ct-10670 32 7 laboratory laboratory NOUN ct-10670 32 8 is be AUX ct-10670 32 9 useful useful ADJ ct-10670 32 10 to to PART ct-10670 32 11 prevent prevent VERB ct-10670 32 12 the the DET ct-10670 32 13 ingress ingress NOUN ct-10670 32 14 of of ADP ct-10670 32 15 airborne airborne ADJ ct-10670 32 16 particles particle NOUN ct-10670 32 17 when when SCONJ ct-10670 32 18 the the DET ct-10670 32 19 entry entry NOUN ct-10670 32 20 door door NOUN ct-10670 32 21 is be AUX ct-10670 32 22 opened open VERB ct-10670 32 23 . . PUNCT ct-10670 33 1 we we PRON ct-10670 33 2 refrain refrain VERB ct-10670 33 3 from from ADP ct-10670 33 4 the the DET ct-10670 33 5 use use NOUN ct-10670 33 6 of of ADP ct-10670 33 7 ethanol ethanol NOUN ct-10670 33 8 - - PUNCT ct-10670 33 9 containing contain VERB ct-10670 33 10 solutions solution NOUN ct-10670 33 11 and and CCONJ ct-10670 33 12 instead instead ADV ct-10670 33 13 , , PUNCT ct-10670 33 14 we we PRON ct-10670 33 15 use use VERB ct-10670 33 16 oosafe oosafe ADJ ct-10670 33 17 ® ® NOUN ct-10670 33 18 disinfectant disinfectant NOUN ct-10670 33 19 ( ( PUNCT ct-10670 33 20 oosafe oosafe PROPN ct-10670 33 21 , , PUNCT ct-10670 33 22 inc inc PROPN ct-10670 33 23 , , PUNCT ct-10670 33 24 hingham hingham PROPN ct-10670 33 25 , , PUNCT ct-10670 33 26 ma ma PROPN ct-10670 33 27 02043 02043 NUM ct-10670 33 28 ) ) PUNCT ct-10670 33 29 for for ADP ct-10670 33 30 cleaning clean VERB ct-10670 33 31 surfaces surface NOUN ct-10670 33 32 . . PUNCT ct-10670 34 1 access access NOUN ct-10670 34 2 is be AUX ct-10670 34 3 restricted restrict VERB ct-10670 34 4 to to ADP ct-10670 34 5 essential essential ADJ ct-10670 34 6 personnel personnel NOUN ct-10670 34 7 , , PUNCT ct-10670 34 8 who who PRON ct-10670 34 9 use use VERB ct-10670 34 10 dedicated dedicated ADJ ct-10670 34 11 sets set NOUN ct-10670 34 12 of of ADP ct-10670 34 13 shoes shoe NOUN ct-10670 34 14 , , PUNCT ct-10670 34 15 scrubs scrub NOUN ct-10670 34 16 , , PUNCT ct-10670 34 17 and and CCONJ ct-10670 34 18 laboratory laboratory NOUN ct-10670 34 19 coats coat NOUN ct-10670 34 20 when when SCONJ ct-10670 34 21 working work VERB ct-10670 34 22 in in ADP ct-10670 34 23 the the DET ct-10670 34 24 icsi icsi ADJ ct-10670 34 25 room room NOUN ct-10670 34 26 . . PUNCT ct-10670 35 1 commonly commonly ADV ct-10670 35 2 , , PUNCT ct-10670 35 3 we we PRON ct-10670 35 4 avoid avoid VERB ct-10670 35 5 using use VERB ct-10670 35 6 gloves glove NOUN ct-10670 35 7 ; ; PUNCT ct-10670 35 8 thus thus ADV ct-10670 35 9 , , PUNCT ct-10670 35 10 rigorous rigorous ADJ ct-10670 35 11 hand hand NOUN ct-10670 35 12 - - PUNCT ct-10670 35 13 scrubbing scrubbing NOUN ct-10670 35 14 is be AUX ct-10670 35 15 required require VERB ct-10670 35 16 before before ADP ct-10670 35 17 entering enter VERB ct-10670 35 18 the the DET ct-10670 35 19 room room NOUN ct-10670 35 20 . . PUNCT ct-10670 36 1 use use NOUN ct-10670 36 2 of of ADP ct-10670 36 3 scented scented ADJ ct-10670 36 4 soap soap NOUN ct-10670 36 5 , , PUNCT ct-10670 36 6 perfume perfume NOUN ct-10670 36 7 , , PUNCT ct-10670 36 8 or or CCONJ ct-10670 36 9 lotions lotion NOUN ct-10670 36 10 is be AUX ct-10670 36 11 forbidden forbid VERB ct-10670 36 12 , , PUNCT ct-10670 36 13 even even ADV ct-10670 36 14 when when SCONJ ct-10670 36 15 not not PART ct-10670 36 16 working work VERB ct-10670 36 17 directly directly ADV ct-10670 36 18 with with ADP ct-10670 36 19 embryos embryo NOUN ct-10670 36 20 / / SYM ct-10670 36 21 gametes gamete NOUN ct-10670 36 22 . . PUNCT ct-10670 37 1 we we PRON ct-10670 37 2 maintain maintain VERB ct-10670 37 3 a a DET ct-10670 37 4 sticky sticky ADJ ct-10670 37 5 floor floor NOUN ct-10670 37 6 mat mat NOUN ct-10670 37 7 by by ADP ct-10670 37 8 the the DET ct-10670 37 9 door door NOUN ct-10670 37 10 to to PART ct-10670 37 11 control control VERB ct-10670 37 12 foot foot NOUN ct-10670 37 13 - - PUNCT ct-10670 37 14 carried carry VERB ct-10670 37 15 lint lint NOUN ct-10670 37 16 and and CCONJ ct-10670 37 17 debris debris NOUN ct-10670 37 18 , , PUNCT ct-10670 37 19 and and CCONJ ct-10670 37 20 end end NOUN ct-10670 37 21 - - PUNCT ct-10670 37 22 of of ADP ct-10670 37 23 - - PUNCT ct-10670 37 24 day day NOUN ct-10670 37 25 daily daily ADV ct-10670 37 26 sweeping sweep VERB ct-10670 37 27 for for ADP ct-10670 37 28 dust dust NOUN ct-10670 37 29 and and CCONJ ct-10670 37 30 debris debris NOUN ct-10670 37 31 using use VERB ct-10670 37 32 a a DET ct-10670 37 33 swiffer swiffer NOUN ct-10670 37 34 floor floor NOUN ct-10670 37 35 mop mop NOUN ct-10670 37 36 with with ADP ct-10670 37 37 dry dry ADJ ct-10670 37 38 pads pad NOUN ct-10670 37 39 sprayed spray VERB ct-10670 37 40 with with ADP ct-10670 37 41 oosafe oosafe ADJ ct-10670 37 42 ® ® NOUN ct-10670 37 43 disinfectant disinfectant NOUN ct-10670 37 44 after after SCONJ ct-10670 37 45 all all DET ct-10670 37 46 incubators incubator NOUN ct-10670 37 47 are be AUX ct-10670 37 48 closed close VERB ct-10670 37 49 for for ADP ct-10670 37 50 the the DET ct-10670 37 51 night night NOUN ct-10670 37 52 . . PUNCT ct-10670 38 1 when when SCONJ ct-10670 38 2 gametes gamete NOUN ct-10670 38 3 or or CCONJ ct-10670 38 4 embryos embryo NOUN ct-10670 38 5 are be AUX ct-10670 38 6 outside outside ADP ct-10670 38 7 the the DET ct-10670 38 8 incubator incubator NOUN ct-10670 38 9 , , PUNCT ct-10670 38 10 dim dim ADJ ct-10670 38 11 light light NOUN ct-10670 38 12 is be AUX ct-10670 38 13 used use VERB ct-10670 38 14 , , PUNCT ct-10670 38 15 and and CCONJ ct-10670 38 16 the the DET ct-10670 38 17 door door NOUN ct-10670 38 18 remains remain VERB ct-10670 38 19 closed closed ADJ ct-10670 38 20 . . PUNCT ct-10670 39 1 science science NOUN ct-10670 39 2 of of ADP ct-10670 39 3 intracytoplasmic intracytoplasmic ADJ ct-10670 39 4 sperm sperm NOUN ct-10670 39 5 injection injection NOUN ct-10670 39 6 in in ADP ct-10670 39 7 broader broad ADJ ct-10670 39 8 terms term NOUN ct-10670 39 9 , , PUNCT ct-10670 39 10 there there PRON ct-10670 39 11 is be VERB ct-10670 39 12 a a DET ct-10670 39 13 universal universal ADJ ct-10670 39 14 scheme scheme NOUN ct-10670 39 15 for for ADP ct-10670 39 16 icsi icsi NOUN ct-10670 39 17 , , PUNCT ct-10670 39 18 regardless regardless ADV ct-10670 39 19 of of ADP ct-10670 39 20 the the DET ct-10670 39 21 protocol protocol NOUN ct-10670 39 22 or or CCONJ ct-10670 39 23 species species NOUN ct-10670 39 24 specificity specificity NOUN ct-10670 39 25 : : PUNCT ct-10670 39 26 gamete gamete NOUN ct-10670 39 27 acquisition acquisition NOUN ct-10670 39 28 , , PUNCT ct-10670 39 29 in in ADP ct-10670 39 30 vitro vitro X ct-10670 39 31 maturation maturation NOUN ct-10670 39 32 ( ( PUNCT ct-10670 39 33 ivm ivm PROPN ct-10670 39 34 ) ) PUNCT ct-10670 39 35 , , PUNCT ct-10670 39 36 sperm sperm NOUN ct-10670 39 37 selection selection NOUN ct-10670 39 38 , , PUNCT ct-10670 39 39 fertilization fertilization NOUN ct-10670 39 40 using use VERB ct-10670 39 41 microinjection microinjection NOUN ct-10670 39 42 of of ADP ct-10670 39 43 a a DET ct-10670 39 44 single single ADJ ct-10670 39 45 sperm sperm NOUN ct-10670 39 46 , , PUNCT ct-10670 39 47 in in ADP ct-10670 39 48 vitro vitro X ct-10670 39 49 culture culture NOUN ct-10670 39 50 ( ( PUNCT ct-10670 39 51 ivc ivc PROPN ct-10670 39 52 ) ) PUNCT ct-10670 39 53 to to ADP ct-10670 39 54 the the DET ct-10670 39 55 blastocyst blastocyst NOUN ct-10670 39 56 stage stage NOUN ct-10670 39 57 , , PUNCT ct-10670 39 58 vitrification vitrification NOUN ct-10670 39 59 ( ( PUNCT ct-10670 39 60 optional optional ADJ ct-10670 39 61 ) ) PUNCT ct-10670 39 62 , , PUNCT ct-10670 39 63 and and CCONJ ct-10670 39 64 embryo embryo NOUN ct-10670 39 65 transfer transfer NOUN ct-10670 39 66 into into ADP ct-10670 39 67 recipient recipient NOUN ct-10670 39 68 mares mare NOUN ct-10670 39 69 . . PUNCT ct-10670 40 1 although although SCONJ ct-10670 40 2 commercial commercial ADJ ct-10670 40 3 media medium NOUN ct-10670 40 4 and and CCONJ ct-10670 40 5 their their PRON ct-10670 40 6 strictly strictly ADV ct-10670 40 7 prescribed prescribe VERB ct-10670 40 8 protocols protocol NOUN ct-10670 40 9 are be AUX ct-10670 40 10 readily readily ADV ct-10670 40 11 available available ADJ ct-10670 40 12 , , PUNCT ct-10670 40 13 embryo embryo NOUN ct-10670 40 14 production production NOUN ct-10670 40 15 methods method NOUN ct-10670 40 16 in in ADP ct-10670 40 17 our our PRON ct-10670 40 18 laboratory laboratory NOUN ct-10670 40 19 are be AUX ct-10670 40 20 based base VERB ct-10670 40 21 on on ADP ct-10670 40 22 previously previously ADV ct-10670 40 23 reported report VERB ct-10670 40 24 equine equine NOUN ct-10670 40 25 culture culture NOUN ct-10670 40 26 systems.11,15 systems.11,15 NOUN ct-10670 40 27 for for ADP ct-10670 40 28 purposes purpose NOUN ct-10670 40 29 of of ADP ct-10670 40 30 this this DET ct-10670 40 31 discussion discussion NOUN ct-10670 40 32 , , PUNCT ct-10670 40 33 we we PRON ct-10670 40 34 refer refer VERB ct-10670 40 35 to to ADP ct-10670 40 36 ‘ ' PUNCT ct-10670 40 37 holding hold VERB ct-10670 40 38 ’ ' PUNCT ct-10670 40 39 medium medium NOUN ct-10670 40 40 as as ADP ct-10670 40 41 an an DET ct-10670 40 42 oocyte oocyte ADJ ct-10670 40 43 transportation transportation NOUN ct-10670 40 44 culture culture NOUN ct-10670 40 45 medium medium NOUN ct-10670 40 46 that that PRON ct-10670 40 47 is be AUX ct-10670 40 48 commercially commercially ADV ct-10670 40 49 available available ADJ ct-10670 40 50 and and CCONJ ct-10670 40 51 used use VERB ct-10670 40 52 for for ADP ct-10670 40 53 oocyte oocyte ADJ ct-10670 40 54 shipping shipping NOUN ct-10670 40 55 to to ADP ct-10670 40 56 our our PRON ct-10670 40 57 laboratory laboratory NOUN ct-10670 40 58 , , PUNCT ct-10670 40 59 and and CCONJ ct-10670 40 60 in in ADP ct-10670 40 61 which which PRON ct-10670 40 62 metabolic metabolic NOUN ct-10670 40 63 state state NOUN ct-10670 40 64 is be AUX ct-10670 40 65 supported support VERB ct-10670 40 66 but but CCONJ ct-10670 40 67 does do AUX ct-10670 40 68 not not PART ct-10670 40 69 allow allow VERB ct-10670 40 70 oocytes oocyte NOUN ct-10670 40 71 to to PART ct-10670 40 72 develop develop VERB ct-10670 40 73 or or CCONJ ct-10670 40 74 otherwise otherwise ADV ct-10670 40 75 mature mature ADJ ct-10670 40 76 . . PUNCT ct-10670 41 1 after after ADP ct-10670 41 2 a a DET ct-10670 41 3 brief brief ADJ ct-10670 41 4 period period NOUN ct-10670 41 5 , , PUNCT ct-10670 41 6 typically typically ADV ct-10670 41 7 24 24 NUM ct-10670 41 8 hours hour NOUN ct-10670 41 9 in in ADP ct-10670 41 10 a a DET ct-10670 41 11 commercial commercial ADJ ct-10670 41 12 holding holding NOUN ct-10670 41 13 medium medium NOUN ct-10670 41 14 , , PUNCT ct-10670 41 15 the the DET ct-10670 41 16 oocytes oocyte NOUN ct-10670 41 17 are be AUX ct-10670 41 18 transferred transfer VERB ct-10670 41 19 to to ADP ct-10670 41 20 a a DET ct-10670 41 21 medium medium NOUN ct-10670 41 22 that that PRON ct-10670 41 23 supports support VERB ct-10670 41 24 ivm ivm PROPN ct-10670 41 25 from from ADP ct-10670 41 26 the the DET ct-10670 41 27 mi mi PROPN ct-10670 41 28 stage stage NOUN ct-10670 41 29 ( ( PUNCT ct-10670 41 30 no no DET ct-10670 41 31 visible visible ADJ ct-10670 41 32 polar polar ADJ ct-10670 41 33 bodies body NOUN ct-10670 41 34 ) ) PUNCT ct-10670 41 35 to to ADP ct-10670 41 36 the the DET ct-10670 41 37 mii mii PROPN ct-10670 41 38 stage stage NOUN ct-10670 41 39 of of ADP ct-10670 41 40 gametogenesis gametogenesis NOUN ct-10670 41 41 , , PUNCT ct-10670 41 42 revealing reveal VERB ct-10670 41 43 a a DET ct-10670 41 44 single single ADJ ct-10670 41 45 polar polar ADJ ct-10670 41 46 body body NOUN ct-10670 41 47 that that PRON ct-10670 41 48 indicates indicate VERB ct-10670 41 49 the the DET ct-10670 41 50 resumption resumption NOUN ct-10670 41 51 of of ADP ct-10670 41 52 meiosis meiosis NOUN ct-10670 41 53 . . PUNCT ct-10670 42 1 at at ADP ct-10670 42 2 this this DET ct-10670 42 3 stage stage NOUN ct-10670 42 4 of of ADP ct-10670 42 5 development development NOUN ct-10670 42 6 , , PUNCT ct-10670 42 7 the the DET ct-10670 42 8 oocyte oocyte NOUN ct-10670 42 9 is be AUX ct-10670 42 10 prepared prepare VERB ct-10670 42 11 to to PART ct-10670 42 12 undergo undergo VERB ct-10670 42 13 fertilization fertilization NOUN ct-10670 42 14 by by ADP ct-10670 42 15 a a DET ct-10670 42 16 single single ADJ ct-10670 42 17 sperm sperm NOUN ct-10670 42 18 . . PUNCT ct-10670 43 1 although although SCONJ ct-10670 43 2 , , PUNCT ct-10670 43 3 many many ADJ ct-10670 43 4 aspirations aspiration NOUN ct-10670 43 5 are be AUX ct-10670 43 6 performed perform VERB ct-10670 43 7 in in ADP ct-10670 43 8 - - PUNCT ct-10670 43 9 house house NOUN ct-10670 43 10 at at ADP ct-10670 43 11 uc uc PROPN ct-10670 43 12 davis davis PROPN ct-10670 43 13 , , PUNCT ct-10670 43 14 our our PRON ct-10670 43 15 labortory labortory NOUN ct-10670 43 16 , , PUNCT ct-10670 43 17 like like ADP ct-10670 43 18 most most ADJ ct-10670 43 19 icsi icsi ADJ ct-10670 43 20 facilities facility NOUN ct-10670 43 21 , , PUNCT ct-10670 43 22 receives receive VERB ct-10670 43 23 numerous numerous ADJ ct-10670 43 24 shipped ship VERB ct-10670 43 25 oocytes oocyte NOUN ct-10670 43 26 from from ADP ct-10670 43 27 referral referral ADJ ct-10670 43 28 practitioners practitioner NOUN ct-10670 43 29 performing perform VERB ct-10670 43 30 aspirations aspiration NOUN ct-10670 43 31 in in ADP ct-10670 43 32 distant distant ADJ ct-10670 43 33 locations location NOUN ct-10670 43 34 . . PUNCT ct-10670 44 1 we we PRON ct-10670 44 2 also also ADV ct-10670 44 3 obtain obtain VERB ct-10670 44 4 oocytes oocyte NOUN ct-10670 44 5 from from ADP ct-10670 44 6 postmortem postmortem PROPN ct-10670 44 7 ovarian ovarian ADJ ct-10670 44 8 retrieval retrieval NOUN ct-10670 44 9 . . PUNCT ct-10670 45 1 after after ADP ct-10670 45 2 ivm ivm PROPN ct-10670 45 3 , , PUNCT ct-10670 45 4 both both PRON ct-10670 45 5 oocytes oocyte NOUN ct-10670 45 6 and and CCONJ ct-10670 45 7 sperm sperm NOUN ct-10670 45 8 are be AUX ct-10670 45 9 evaluated evaluate VERB ct-10670 45 10 . . PUNCT ct-10670 46 1 oocytes oocyte NOUN ct-10670 46 2 that that PRON ct-10670 46 3 extruded extrude VERB ct-10670 46 4 their their PRON ct-10670 46 5 first first ADJ ct-10670 46 6 polar polar ADJ ct-10670 46 7 body body NOUN ct-10670 46 8 and and CCONJ ct-10670 46 9 motile motile ADJ ct-10670 46 10 sperm sperm NOUN ct-10670 46 11 of of ADP ct-10670 46 12 normal normal ADJ ct-10670 46 13 morphology morphology NOUN ct-10670 46 14 are be AUX ct-10670 46 15 then then ADV ct-10670 46 16 moved move VERB ct-10670 46 17 into into ADP ct-10670 46 18 the the DET ct-10670 46 19 icsi icsi ADJ ct-10670 46 20 dish dish NOUN ct-10670 46 21 and and CCONJ ct-10670 46 22 the the DET ct-10670 46 23 microinjection microinjection NOUN ct-10670 46 24 procedure procedure NOUN ct-10670 46 25 is be AUX ct-10670 46 26 performed perform VERB ct-10670 46 27 by by ADP ct-10670 46 28 an an DET ct-10670 46 29 embryologist embryologist NOUN ct-10670 46 30 . . PUNCT ct-10670 47 1 in in ADP ct-10670 47 2 a a DET ct-10670 47 3 typical typical ADJ ct-10670 47 4 session session NOUN ct-10670 47 5 , , PUNCT ct-10670 47 6 the the DET ct-10670 47 7 embryo embryo NOUN ct-10670 47 8 is be AUX ct-10670 47 9 vitrified vitrify VERB ct-10670 47 10 when when SCONJ ct-10670 47 11 it it PRON ct-10670 47 12 reaches reach VERB ct-10670 47 13 the the DET ct-10670 47 14 blastocyst blastocyst NOUN ct-10670 47 15 stage stage NOUN ct-10670 47 16 of of ADP ct-10670 47 17 development development NOUN ct-10670 47 18 at at ADP ct-10670 47 19 7 7 NUM ct-10670 47 20 to to PART ct-10670 47 21 10 10 NUM ct-10670 47 22 days day NOUN ct-10670 47 23 . . PUNCT ct-10670 48 1 vitrified vitrify VERB ct-10670 48 2 embryos embryo NOUN ct-10670 48 3 are be AUX ct-10670 48 4 then then ADV ct-10670 48 5 express express ADV ct-10670 48 6 - - PUNCT ct-10670 48 7 shipped ship VERB ct-10670 48 8 to to ADP ct-10670 48 9 veterinarians veterinarian NOUN ct-10670 48 10 managing manage VERB ct-10670 48 11 recipient recipient NOUN ct-10670 48 12 mares mare NOUN ct-10670 48 13 for for ADP ct-10670 48 14 thawing thaw VERB ct-10670 48 15 and and CCONJ ct-10670 48 16 embryo embryo NOUN ct-10670 48 17 transfer transfer NOUN ct-10670 48 18 . . PUNCT ct-10670 49 1 in in ADP ct-10670 49 2 special special ADJ ct-10670 49 3 circumstances circumstance NOUN ct-10670 49 4 , , PUNCT ct-10670 49 5 alternative alternative ADJ ct-10670 49 6 options option NOUN ct-10670 49 7 are be AUX ct-10670 49 8 available available ADJ ct-10670 49 9 depending depend VERB ct-10670 49 10 on on ADP ct-10670 49 11 the the DET ct-10670 49 12 animal animal NOUN ct-10670 49 13 ’s ’s PART ct-10670 49 14 status status NOUN ct-10670 49 15 . . PUNCT ct-10670 50 1 although although SCONJ ct-10670 50 2 rare rare ADJ ct-10670 50 3 , , PUNCT ct-10670 50 4 we we PRON ct-10670 50 5 retrieve retrieve VERB ct-10670 50 6 ovaries ovary NOUN ct-10670 50 7 at at ADP ct-10670 50 8 the the DET ct-10670 50 9 time time NOUN ct-10670 50 10 of of ADP ct-10670 50 11 or or CCONJ ct-10670 50 12 shortly shortly ADV ct-10670 50 13 after after ADP ct-10670 50 14 euthanasia euthanasia NOUN ct-10670 50 15 . . PUNCT ct-10670 51 1 inevitably inevitably ADV ct-10670 51 2 , , PUNCT ct-10670 51 3 postmortem postmortem NOUN ct-10670 51 4 oocyte oocyte NOUN ct-10670 51 5 recovery recovery NOUN ct-10670 51 6 has have VERB ct-10670 51 7 a a DET ct-10670 51 8 negative negative ADJ ct-10670 51 9 impact impact NOUN ct-10670 51 10 on on ADP ct-10670 51 11 the the DET ct-10670 51 12 quality quality NOUN ct-10670 51 13 of of ADP ct-10670 51 14 oocytes oocyte NOUN ct-10670 51 15 that that PRON ct-10670 51 16 can can AUX ct-10670 51 17 be be AUX ct-10670 51 18 reflected reflect VERB ct-10670 51 19 downstream downstream ADV ct-10670 51 20 on on ADP ct-10670 51 21 the the DET ct-10670 51 22 success success NOUN ct-10670 51 23 of of ADP ct-10670 51 24 the the DET ct-10670 51 25 icsi icsi ADJ ct-10670 51 26 procedure procedure NOUN ct-10670 51 27 . . PUNCT ct-10670 52 1 the the DET ct-10670 52 2 most most ADV ct-10670 52 3 critical critical ADJ ct-10670 52 4 factors factor NOUN ct-10670 52 5 for for ADP ct-10670 52 6 icsi icsi ADJ ct-10670 52 7 success success NOUN ct-10670 52 8 in in ADP ct-10670 52 9 such such ADJ ct-10670 52 10 cases case NOUN ct-10670 52 11 were be AUX ct-10670 52 12 the the DET ct-10670 52 13 number number NOUN ct-10670 52 14 of of ADP ct-10670 52 15 collected collect VERB ct-10670 52 16 oocytes oocyte NOUN ct-10670 52 17 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PRON ct-10670 52 18 citation citation NOUN ct-10670 52 19 : : PUNCT ct-10670 52 20 clinical clinical ADJ ct-10670 52 21 theriogenology theriogenology NOUN ct-10670 52 22 2024 2024 NUM ct-10670 52 23 , , PUNCT ct-10670 52 24 16 16 NUM ct-10670 52 25 , , PUNCT ct-10670 52 26 10670 10670 NUM ct-10670 52 27 , , PUNCT ct-10670 52 28 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 ADP ct-10670 52 29 3 3 NUM ct-10670 52 30 and and CCONJ ct-10670 52 31 euthanasia euthanasia NOUN ct-10670 52 32 being be AUX ct-10670 52 33 due due ADJ ct-10670 52 34 to to ADP ct-10670 52 35 a a DET ct-10670 52 36 nonacute nonacute ADJ ct-10670 52 37 medical medical ADJ ct-10670 52 38 condition.16 condition.16 PROPN ct-10670 52 39 furthermore furthermore ADV ct-10670 52 40 , , PUNCT ct-10670 52 41 pentobarbital pentobarbital NOUN ct-10670 52 42 penetrates penetrate VERB ct-10670 52 43 follicles follicle NOUN ct-10670 52 44 ( ( PUNCT ct-10670 52 45 detectable detectable ADJ ct-10670 52 46 soon soon ADV ct-10670 52 47 after after ADP ct-10670 52 48 injection injection NOUN ct-10670 52 49 ) ) PUNCT ct-10670 52 50 , , PUNCT ct-10670 52 51 and and CCONJ ct-10670 52 52 our our PRON ct-10670 52 53 research research NOUN ct-10670 52 54 suggested suggest VERB ct-10670 52 55 that that SCONJ ct-10670 52 56 this this PRON ct-10670 52 57 may may AUX ct-10670 52 58     SPACE ct-10670 52 59 impede impede VERB ct-10670 52 60 both both CCONJ ct-10670 52 61 bovine bovine ADJ ct-10670 52 62 and and CCONJ ct-10670 52 63 equine equine NOUN ct-10670 52 64 in in ADP ct-10670 52 65 vitro vitro X ct-10670 52 66 embryo embryo NOUN ct-10670 52 67 production.17 production.17 NOUN ct-10670 52 68 acquiring acquire VERB ct-10670 52 69 gametes gamete NOUN ct-10670 52 70 for for ADP ct-10670 52 71 intracytoplasmic intracytoplasmic ADJ ct-10670 52 72 sperm sperm NOUN ct-10670 52 73 injection injection NOUN ct-10670 52 74 transvaginal transvaginal NOUN ct-10670 52 75 aspiration aspiration NOUN ct-10670 52 76 of of ADP ct-10670 52 77 oocytes oocyte NOUN ct-10670 52 78 transvaginal transvaginal ADJ ct-10670 52 79 aspiration aspiration NOUN ct-10670 52 80 ( ( PUNCT ct-10670 52 81 tva tva PROPN ct-10670 52 82 ) ) PUNCT ct-10670 52 83 of of ADP ct-10670 52 84 oocytes oocyte NOUN ct-10670 52 85 or or CCONJ ct-10670 52 86 ovum ovum NOUN ct-10670 52 87 pick pick NOUN ct-10670 52 88 - - PUNCT ct-10670 52 89 up up ADP ct-10670 52 90 ( ( PUNCT ct-10670 52 91 opu opu NOUN ct-10670 52 92 ) ) PUNCT ct-10670 52 93 is be AUX ct-10670 52 94 necessary necessary ADJ ct-10670 52 95 to to PART ct-10670 52 96 obtain obtain VERB ct-10670 52 97 oocytes oocyte NOUN ct-10670 52 98 from from ADP ct-10670 52 99 ovarian ovarian ADJ ct-10670 52 100 follicles follicle NOUN ct-10670 52 101 . . PUNCT ct-10670 53 1 it it PRON ct-10670 53 2 is be AUX ct-10670 53 3 performed perform VERB ct-10670 53 4 on on ADP ct-10670 53 5 standing stand VERB ct-10670 53 6 , , PUNCT ct-10670 53 7 sedated sedated ADJ ct-10670 53 8 mares mare NOUN ct-10670 53 9 , , PUNCT ct-10670 53 10 using use VERB ct-10670 53 11 an an DET ct-10670 53 12 ultrasound ultrasound NOUN ct-10670 53 13 - - PUNCT ct-10670 53 14 guided guide VERB ct-10670 53 15 transvaginal transvaginal ADJ ct-10670 53 16 probe probe NOUN ct-10670 53 17 with with ADP ct-10670 53 18 needle needle NOUN ct-10670 53 19 channel channel NOUN ct-10670 53 20 attached attach VERB ct-10670 53 21 to to ADP ct-10670 53 22 an an DET ct-10670 53 23 exapad exapad NOUN ct-10670 53 24 ultrasound ultrasound NOUN ct-10670 53 25 scanner scanner NOUN ct-10670 53 26 ( ( PUNCT ct-10670 53 27 imv imv PROPN ct-10670 53 28 technologies technologies PROPN ct-10670 53 29 , , PUNCT ct-10670 53 30 inc inc PROPN ct-10670 53 31 . . PROPN ct-10670 53 32 ) ) PUNCT ct-10670 53 33 . . PUNCT ct-10670 54 1 our our PRON ct-10670 54 2 group group NOUN ct-10670 54 3 uses use VERB ct-10670 54 4 a a DET ct-10670 54 5 rocket rocket NOUN ct-10670 54 6 craft craft NOUN ct-10670 54 7 duo duo NOUN ct-10670 54 8 - - PUNCT ct-10670 54 9 vac vac NOUN ct-10670 54 10 vacuum vacuum NOUN ct-10670 54 11 suction suction NOUN ct-10670 54 12 pump pump NOUN ct-10670 54 13 ( ( PUNCT ct-10670 54 14 rocket rocket PROPN ct-10670 54 15 medical medical PROPN ct-10670 54 16 plc plc PROPN ct-10670 54 17 , , PUNCT ct-10670 54 18 uk uk PROPN ct-10670 54 19 ) ) PUNCT ct-10670 54 20 connected connect VERB ct-10670 54 21 to to ADP ct-10670 54 22 a a DET ct-10670 54 23 12 12 NUM ct-10670 54 24 g g NOUN ct-10670 54 25 x x SYM ct-10670 54 26 25 25 NUM ct-10670 54 27 ” " PUNCT ct-10670 54 28 double double ADJ ct-10670 54 29 - - PUNCT ct-10670 54 30 lumen luman NOUN ct-10670 54 31 needle needle NOUN ct-10670 54 32 that that PRON ct-10670 54 33 is be AUX ct-10670 54 34 manually manually ADV ct-10670 54 35 directed direct VERB ct-10670 54 36 into into ADP ct-10670 54 37 each each DET ct-10670 54 38 follicle follicle NOUN ct-10670 54 39 ( ( PUNCT ct-10670 54 40 5 5 NUM ct-10670 54 41 - - SYM ct-10670 54 42 20 20 NUM ct-10670 54 43 mm mm NOUN ct-10670 54 44 diameter diameter NOUN ct-10670 54 45 ) ) PUNCT ct-10670 54 46 which which PRON ct-10670 54 47 is be AUX ct-10670 54 48 punctured puncture VERB ct-10670 54 49 and and CCONJ ct-10670 54 50 followed follow VERB ct-10670 54 51 by by ADP ct-10670 54 52 manual manual ADJ ct-10670 54 53 needle needle NOUN ct-10670 54 54 agitation agitation NOUN ct-10670 54 55 ( ( PUNCT ct-10670 54 56 necessary necessary ADJ ct-10670 54 57 to to PART ct-10670 54 58 scrape scrape VERB ct-10670 54 59 the the DET ct-10670 54 60 follicular follicular ADJ ct-10670 54 61 wall wall NOUN ct-10670 54 62 ) ) PUNCT ct-10670 54 63 accompanying accompany VERB ct-10670 54 64 follicular follicular ADJ ct-10670 54 65 lavage lavage NOUN ct-10670 54 66 . . PUNCT ct-10670 55 1 each each DET ct-10670 55 2 follicle follicle NOUN ct-10670 55 3 is be AUX ct-10670 55 4 lavaged lavage VERB ct-10670 55 5 by by ADP ct-10670 55 6 injecting inject VERB ct-10670 55 7 a a DET ct-10670 55 8 small small ADJ ct-10670 55 9 volume volume NOUN ct-10670 55 10 adequate adequate ADJ ct-10670 55 11 to to PART ct-10670 55 12 visibly visibly ADV ct-10670 55 13 expand expand VERB ct-10670 55 14 the the DET ct-10670 55 15 follicle follicle NOUN ct-10670 55 16 and and CCONJ ct-10670 55 17 subsequently subsequently ADV ct-10670 55 18 draining drain VERB ct-10670 55 19 the the DET ct-10670 55 20 fluid fluid NOUN ct-10670 55 21 while while SCONJ ct-10670 55 22 observing observe VERB ct-10670 55 23 the the DET ct-10670 55 24 ultrasound ultrasound ADJ ct-10670 55 25 flat flat ADJ ct-10670 55 26 - - PUNCT ct-10670 55 27 screen screen NOUN ct-10670 55 28 display display NOUN ct-10670 55 29 . . PUNCT ct-10670 56 1 although although SCONJ ct-10670 56 2 numerous numerous ADJ ct-10670 56 3 factors factor NOUN ct-10670 56 4 contribute contribute VERB ct-10670 56 5 to to ADP ct-10670 56 6 variations variation NOUN ct-10670 56 7 in in ADP ct-10670 56 8 pump pump NOUN ct-10670 56 9 settings setting NOUN ct-10670 56 10 ( ( PUNCT ct-10670 56 11 length length NOUN ct-10670 56 12 and and CCONJ ct-10670 56 13 diameter diameter NOUN ct-10670 56 14 of of ADP ct-10670 56 15 tubing tubing NOUN ct-10670 56 16 , , PUNCT ct-10670 56 17 needle needle NOUN ct-10670 56 18 length length NOUN ct-10670 56 19 and and CCONJ ct-10670 56 20 inner inner ADJ ct-10670 56 21 diameter diameter NOUN ct-10670 56 22 , , PUNCT ct-10670 56 23 and and CCONJ ct-10670 56 24 mare mare NOUN ct-10670 56 25 ’s ’s PART ct-10670 56 26 movement movement NOUN ct-10670 56 27 / / SYM ct-10670 56 28 sedation sedation NOUN ct-10670 56 29 status status NOUN ct-10670 56 30 ) ) PUNCT ct-10670 56 31 , , PUNCT ct-10670 56 32 we we PRON ct-10670 56 33 typically typically ADV ct-10670 56 34 set set VERB ct-10670 56 35 the the DET ct-10670 56 36 pump pump NOUN ct-10670 56 37 to to PART ct-10670 56 38 aspirate aspirate VERB ct-10670 56 39 at at ADP ct-10670 56 40 a a DET ct-10670 56 41 flow flow NOUN ct-10670 56 42 rate rate NOUN ct-10670 56 43 of of ADP ct-10670 56 44 20 20 NUM ct-10670 56 45 ml/40 ml/40 PROPN ct-10670 56 46 seconds second NOUN ct-10670 56 47 , , PUNCT ct-10670 56 48 but but CCONJ ct-10670 56 49 the the DET ct-10670 56 50 pressure pressure NOUN ct-10670 56 51 measurement measurement NOUN ct-10670 56 52 can can AUX ct-10670 56 53 vary vary VERB ct-10670 56 54 under under ADP ct-10670 56 55 those those DET ct-10670 56 56 circumstances circumstance NOUN ct-10670 56 57 . . PUNCT ct-10670 57 1 follicles follicle NOUN ct-10670 57 2 > > X ct-10670 57 3 20 20 NUM ct-10670 57 4 mm mm NOUN ct-10670 57 5 diameter diameter NOUN ct-10670 57 6 are be AUX ct-10670 57 7 aspirated aspirate VERB ct-10670 57 8 after after ADP ct-10670 57 9 the the DET ct-10670 57 10 small small ADJ ct-10670 57 11 to to PART ct-10670 57 12 medium medium ADJ ct-10670 57 13 follicles follicle NOUN ct-10670 57 14 ( ( PUNCT ct-10670 57 15 5 5 NUM ct-10670 57 16 - - SYM ct-10670 57 17 20 20 NUM ct-10670 57 18 mm mm NOUN ct-10670 57 19 ) ) PUNCT ct-10670 57 20 , , PUNCT ct-10670 57 21 and and CCONJ ct-10670 57 22 as as SCONJ ct-10670 57 23 the the DET ct-10670 57 24 collapse collapse NOUN ct-10670 57 25 of of ADP ct-10670 57 26 larger large ADJ ct-10670 57 27 follicles follicle NOUN ct-10670 57 28 distorts distort VERB ct-10670 57 29 the the DET ct-10670 57 30 ovarian ovarian ADJ ct-10670 57 31 size size NOUN ct-10670 57 32 and and CCONJ ct-10670 57 33 shape shape NOUN ct-10670 57 34 , , PUNCT ct-10670 57 35 they they PRON ct-10670 57 36 are be AUX ct-10670 57 37 best well ADV ct-10670 57 38 aspirated aspirate VERB ct-10670 57 39 last last ADJ ct-10670 57 40 to to PART ct-10670 57 41 optimize optimize VERB ct-10670 57 42 the the DET ct-10670 57 43 smaller small ADJ ct-10670 57 44 follicle follicle NOUN ct-10670 57 45 aspiration aspiration NOUN ct-10670 57 46 . . PUNCT ct-10670 58 1 although although SCONJ ct-10670 58 2 mares mare NOUN ct-10670 58 3 with with ADP ct-10670 58 4 very very ADV ct-10670 58 5 active active ADJ ct-10670 58 6 ovaries ovary NOUN ct-10670 58 7 with with ADP ct-10670 58 8 small small ADJ ct-10670 58 9 to to PART ct-10670 58 10 medium medium ADJ ct-10670 58 11 antral antral ADJ ct-10670 58 12 follicles follicle NOUN ct-10670 58 13 are be AUX ct-10670 58 14 recommended recommend VERB ct-10670 58 15 as as ADP ct-10670 58 16 oocyte oocyte ADJ ct-10670 58 17 donors donor NOUN ct-10670 58 18 ( ( PUNCT ct-10670 58 19 10 10 NUM ct-10670 58 20 - - SYM ct-10670 58 21 15 15 NUM ct-10670 58 22 follicles follicle NOUN ct-10670 58 23 of of ADP ct-10670 58 24 5 5 NUM ct-10670 58 25 - - SYM ct-10670 58 26 20 20 NUM ct-10670 58 27 mm mm PROPN ct-10670 58 28 diameter diameter NOUN ct-10670 58 29 ) ) PUNCT ct-10670 58 30 , , PUNCT ct-10670 58 31 this this PRON ct-10670 58 32 is be AUX ct-10670 58 33 not not PART ct-10670 58 34 always always ADV ct-10670 58 35 possible possible ADJ ct-10670 58 36 . . PUNCT ct-10670 59 1 when when SCONJ ct-10670 59 2 less less ADJ ct-10670 59 3 than than ADP ct-10670 59 4 an an DET ct-10670 59 5 optimal optimal ADJ ct-10670 59 6 number number NOUN ct-10670 59 7 of of ADP ct-10670 59 8 follicles follicle NOUN ct-10670 59 9 are be AUX ct-10670 59 10 visualized visualize VERB ct-10670 59 11 ( ( PUNCT ct-10670 59 12 typically typically ADV ct-10670 59 13 < < X ct-10670 59 14 5 5 NUM ct-10670 59 15 - - SYM ct-10670 59 16 10 10 NUM ct-10670 59 17 visible visible ADJ ct-10670 59 18 follicles follicle NOUN ct-10670 59 19 ) ) PUNCT ct-10670 59 20 , , PUNCT ct-10670 59 21 we we PRON ct-10670 59 22 recommend recommend VERB ct-10670 59 23 postponing postpone VERB ct-10670 59 24 transvaginal transvaginal ADJ ct-10670 59 25 aspiration aspiration NOUN ct-10670 59 26 of of ADP ct-10670 59 27 oocytes oocyte NOUN ct-10670 59 28 by by ADP ct-10670 59 29 2 2 NUM ct-10670 59 30 weeks week NOUN ct-10670 59 31 to to PART ct-10670 59 32 allow allow VERB ct-10670 59 33 for for ADP ct-10670 59 34 the the DET ct-10670 59 35 mare mare NOUN ct-10670 59 36 ’s ’s PART ct-10670 59 37 natural natural ADJ ct-10670 59 38 cycle cycle NOUN ct-10670 59 39 to to PART ct-10670 59 40 recover recover VERB ct-10670 59 41 and and CCONJ ct-10670 59 42 generate generate VERB ct-10670 59 43 a a DET ct-10670 59 44 new new ADJ ct-10670 59 45 cohort cohort NOUN ct-10670 59 46 of of ADP ct-10670 59 47 aspiratable aspiratable ADJ ct-10670 59 48 follicles follicle NOUN ct-10670 59 49 , , PUNCT ct-10670 59 50 and and CCONJ ct-10670 59 51 then then ADV ct-10670 59 52 rechecking rechecke VERB ct-10670 59 53 the the DET ct-10670 59 54 mare mare NOUN ct-10670 59 55 ’s ’s PART ct-10670 59 56 ovarian ovarian ADJ ct-10670 59 57 activity.18 activity.18 PROPN ct-10670 59 58 - - PUNCT ct-10670 59 59 20 20 NUM ct-10670 59 60 however however ADV ct-10670 59 61 , , PUNCT ct-10670 59 62 with with ADP ct-10670 59 63 owner owner NOUN ct-10670 59 64 consent consent NOUN ct-10670 59 65 , , PUNCT ct-10670 59 66 we we PRON ct-10670 59 67 occasionally occasionally ADV ct-10670 59 68 schedule schedule VERB ct-10670 59 69 mares mare NOUN ct-10670 59 70 with with ADP ct-10670 59 71 ~ ~ SYM ct-10670 59 72 3 3 NUM ct-10670 59 73 immature immature ADJ ct-10670 59 74 follicles follicle NOUN ct-10670 59 75 of of ADP ct-10670 59 76 5 5 NUM ct-10670 59 77 - - SYM ct-10670 59 78 20 20 NUM ct-10670 59 79 mm mm NOUN ct-10670 59 80 diameter diameter NOUN ct-10670 59 81 ; ; PUNCT ct-10670 59 82 we we PRON ct-10670 59 83 make make VERB ct-10670 59 84 sure sure ADJ ct-10670 59 85 the the DET ct-10670 59 86 owner owner NOUN ct-10670 59 87 understands understand VERB ct-10670 59 88 that that SCONJ ct-10670 59 89 these these DET ct-10670 59 90 mares mare NOUN ct-10670 59 91 typically typically ADV ct-10670 59 92 yield yield VERB ct-10670 59 93 lower low ADJ ct-10670 59 94 numbers number NOUN ct-10670 59 95 of of ADP ct-10670 59 96 transferrable transferrable ADJ ct-10670 59 97 embryos embryo NOUN ct-10670 59 98 . . PUNCT ct-10670 60 1 for for ADP ct-10670 60 2 optimal optimal ADJ ct-10670 60 3 results result NOUN ct-10670 60 4 , , PUNCT ct-10670 60 5 which which PRON ct-10670 60 6 include include VERB ct-10670 60 7 at at ADV ct-10670 60 8 least least ADV ct-10670 60 9 1 1 NUM ct-10670 60 10 transferrable transferrable ADJ ct-10670 60 11 blastocyst blastocyst NOUN ct-10670 60 12 , , PUNCT ct-10670 60 13 our our PRON ct-10670 60 14 recommendations recommendation NOUN ct-10670 60 15 are be AUX ct-10670 60 16 that that PRON ct-10670 60 17 between between ADP ct-10670 60 18 both both DET ct-10670 60 19 ovaries ovary NOUN ct-10670 60 20 , , PUNCT ct-10670 60 21 at at ADP ct-10670 60 22 least least ADJ ct-10670 60 23 12 12 NUM ct-10670 60 24 follicles follicle NOUN ct-10670 60 25 with with ADP ct-10670 60 26 10 10 NUM ct-10670 60 27 - - SYM ct-10670 60 28 20 20 NUM ct-10670 60 29 mm mm NOUN ct-10670 60 30 diameter diameter NOUN ct-10670 60 31 be be AUX ct-10670 60 32 visualized visualize VERB ct-10670 60 33 at at ADP ct-10670 60 34 the the DET ct-10670 60 35 day day NOUN ct-10670 60 36 of of ADP ct-10670 60 37 aspiration aspiration NOUN ct-10670 60 38 . . PUNCT ct-10670 61 1 regardless regardless ADV ct-10670 61 2 of of ADP ct-10670 61 3 the the DET ct-10670 61 4 outcome outcome NOUN ct-10670 61 5 , , PUNCT ct-10670 61 6 at at ADP ct-10670 61 7 least least ADV ct-10670 61 8 14 14 NUM ct-10670 61 9 days day NOUN ct-10670 61 10 between between ADP ct-10670 61 11 sequential sequential ADJ ct-10670 61 12 aspirations aspiration NOUN ct-10670 61 13 are be AUX ct-10670 61 14 required require VERB ct-10670 61 15 for for ADP ct-10670 61 16 health health NOUN ct-10670 61 17 and and CCONJ ct-10670 61 18 safety safety NOUN ct-10670 61 19 concerns concern NOUN ct-10670 61 20 for for ADP ct-10670 61 21 donor donor NOUN ct-10670 61 22 mares mare NOUN ct-10670 61 23 . . PUNCT ct-10670 62 1 during during ADP ct-10670 62 2 that that DET ct-10670 62 3 period period NOUN ct-10670 62 4 , , PUNCT ct-10670 62 5 a a DET ct-10670 62 6 new new ADJ ct-10670 62 7 wave wave NOUN ct-10670 62 8 of of ADP ct-10670 62 9 follicles follicle NOUN ct-10670 62 10 can can AUX ct-10670 62 11 develop develop VERB ct-10670 62 12 as as ADV ct-10670 62 13 well well ADV ct-10670 62 14 . . PUNCT ct-10670 63 1 we we PRON ct-10670 63 2 can can AUX ct-10670 63 3 obtain obtain VERB ct-10670 63 4 oocytes oocyte NOUN ct-10670 63 5 from from ADP ct-10670 63 6 antral antral ADJ ct-10670 63 7 follicles follicle NOUN ct-10670 63 8 throughout throughout ADP ct-10670 63 9 the the DET ct-10670 63 10 year year NOUN ct-10670 63 11 , , PUNCT ct-10670 63 12 including include VERB ct-10670 63 13 fall fall NOUN ct-10670 63 14 and and CCONJ ct-10670 63 15 spring spring NOUN ct-10670 63 16 transition transition NOUN ct-10670 63 17 periods period NOUN ct-10670 63 18 , , PUNCT ct-10670 63 19 but but CCONJ ct-10670 63 20 this this PRON ct-10670 63 21     SPACE ct-10670 63 22 is be AUX ct-10670 63 23 not not PART ct-10670 63 24 possible possible ADJ ct-10670 63 25 from from ADP ct-10670 63 26 most most ADJ ct-10670 63 27 winter winter NOUN ct-10670 63 28 anestrous anestrous ADJ ct-10670 63 29 mares mare NOUN ct-10670 63 30 in in ADP ct-10670 63 31 northern northern ADJ ct-10670 63 32 california california PROPN ct-10670 63 33 . . PUNCT ct-10670 64 1 for for ADP ct-10670 64 2 a a DET ct-10670 64 3 tva tva NOUN ct-10670 64 4 procedure procedure NOUN ct-10670 64 5 at at ADP ct-10670 64 6 our our PRON ct-10670 64 7 facilities facility NOUN ct-10670 64 8 , , PUNCT ct-10670 64 9 mares mare NOUN ct-10670 64 10 are be AUX ct-10670 64 11 placed place VERB ct-10670 64 12 into into ADP ct-10670 64 13 a a DET ct-10670 64 14 well well ADV ct-10670 64 15 - - PUNCT ct-10670 64 16 padded pad VERB ct-10670 64 17 stock stock NOUN ct-10670 64 18 for for ADP ct-10670 64 19 standing stand VERB ct-10670 64 20 restraint restraint NOUN ct-10670 64 21 . . PUNCT ct-10670 65 1 perineum perineum NOUN ct-10670 65 2 , , PUNCT ct-10670 65 3 vulva vulva NOUN ct-10670 65 4 , , PUNCT ct-10670 65 5 and and CCONJ ct-10670 65 6 tail tail NOUN ct-10670 65 7 base base NOUN ct-10670 65 8 are be AUX ct-10670 65 9 aseptically aseptically ADV ct-10670 65 10 scrubbed scrub VERB ct-10670 65 11 using use VERB ct-10670 65 12 a a DET ct-10670 65 13 mild mild ADJ ct-10670 65 14 soap soap NOUN ct-10670 65 15 such such ADJ ct-10670 65 16 as as ADP ct-10670 65 17 ivory ivory PROPN ct-10670 65 18 soap soap PROPN ct-10670 65 19 . . PUNCT ct-10670 66 1 detomidine detomidine PROPN ct-10670 66 2 ( ( PUNCT ct-10670 66 3 0.01 0.01 NUM ct-10670 66 4 - - PUNCT ct-10670 66 5 0.02 0.02 NUM ct-10670 66 6 mg mg NUM ct-10670 66 7 / / SYM ct-10670 66 8 kg kg NOUN ct-10670 66 9 ) ) PUNCT ct-10670 66 10 and and CCONJ ct-10670 66 11 butorphanol butorphanol VERB ct-10670 66 12 ( ( PUNCT ct-10670 66 13 0.01 0.01 NUM ct-10670 66 14 - - PUNCT ct-10670 66 15 0.02 0.02 NUM ct-10670 66 16 mg mg NUM ct-10670 66 17 / / SYM ct-10670 66 18 kg kg NOUN ct-10670 66 19 ) ) PUNCT ct-10670 66 20 are be AUX ct-10670 66 21 used use VERB ct-10670 66 22 for for ADP ct-10670 66 23 standing stand VERB ct-10670 66 24 sedation sedation NOUN ct-10670 66 25 . . PUNCT ct-10670 67 1 intravenous intravenous ADJ ct-10670 67 2 buscopan buscopan NOUN ct-10670 67 3 ( ( PUNCT ct-10670 67 4 0.32 0.32 NUM ct-10670 67 5 mg mg PROPN ct-10670 67 6 / / SYM ct-10670 67 7 kg kg PROPN ct-10670 67 8 ) ) PUNCT ct-10670 67 9 is be AUX ct-10670 67 10 given give VERB ct-10670 67 11 to to PART ct-10670 67 12 further far ADV ct-10670 67 13 relax relax VERB ct-10670 67 14 the the DET ct-10670 67 15 rectum rectum NOUN ct-10670 67 16 . . PUNCT ct-10670 68 1 the the DET ct-10670 68 2 procedure procedure NOUN ct-10670 68 3 takes take VERB ct-10670 68 4 30 30 NUM ct-10670 68 5 - - SYM ct-10670 68 6 45 45 NUM ct-10670 68 7 minutes minute NOUN ct-10670 68 8 , , PUNCT ct-10670 68 9 but but CCONJ ct-10670 68 10 mares mare NOUN ct-10670 68 11 are be AUX ct-10670 68 12 usually usually ADV ct-10670 68 13 monitored monitor VERB ct-10670 68 14 overnight overnight ADV ct-10670 68 15 prior prior ADV ct-10670 68 16 to to ADP ct-10670 68 17 release release NOUN ct-10670 68 18 from from ADP ct-10670 68 19 the the DET ct-10670 68 20 hospital hospital NOUN ct-10670 68 21 . . PUNCT ct-10670 69 1 although although SCONJ ct-10670 69 2 safe safe ADJ ct-10670 69 3 , , PUNCT ct-10670 69 4 our our PRON ct-10670 69 5 research research NOUN ct-10670 69 6 revealed reveal VERB ct-10670 69 7 that that SCONJ ct-10670 69 8 there there PRON ct-10670 69 9 was be VERB ct-10670 69 10 a a DET ct-10670 69 11 significant significant ADJ ct-10670 69 12 transitory transitory ADJ ct-10670 69 13 increase increase NOUN ct-10670 69 14 in in ADP ct-10670 69 15 serum serum NOUN ct-10670 69 16 lactate lactate NOUN ct-10670 69 17 , , PUNCT ct-10670 69 18 total total ADJ ct-10670 69 19 protein protein NOUN ct-10670 69 20 , , PUNCT ct-10670 69 21 and and CCONJ ct-10670 69 22 peritoneal peritoneal ADJ ct-10670 69 23 neutrophil neutrophil ADJ ct-10670 69 24 count count NOUN ct-10670 69 25 after after ADP ct-10670 69 26 tva tva PROPN ct-10670 69 27 . . PUNCT ct-10670 70 1 furthermore furthermore ADV ct-10670 70 2 , , PUNCT ct-10670 70 3 mares mare NOUN ct-10670 70 4 can can AUX ct-10670 70 5 develop develop VERB ct-10670 70 6 low low ADJ ct-10670 70 7 - - PUNCT ct-10670 70 8 grade grade NOUN ct-10670 70 9 fever fever NOUN ct-10670 70 10 , , PUNCT ct-10670 70 11 mild mild ADJ ct-10670 70 12 colic colic NOUN ct-10670 70 13 , , PUNCT ct-10670 70 14 and and CCONJ ct-10670 70 15 transient transient ADJ ct-10670 70 16 anorexia.21 anorexia.21 NOUN ct-10670 70 17 from from ADP ct-10670 70 18 a a DET ct-10670 70 19 safe safe ADJ ct-10670 70 20 practice practice NOUN ct-10670 70 21 perspective perspective NOUN ct-10670 70 22 , , PUNCT ct-10670 70 23 we we PRON ct-10670 70 24 recommend recommend VERB ct-10670 70 25 24 24 NUM ct-10670 70 26 hours hour NOUN ct-10670 70 27 posttva posttva NOUN ct-10670 70 28 monitoring monitoring NOUN ct-10670 70 29 of of ADP ct-10670 70 30 all all DET ct-10670 70 31 mares mare NOUN ct-10670 70 32 undergoing undergo VERB ct-10670 70 33 the the DET ct-10670 70 34 procedure procedure NOUN ct-10670 70 35 for for ADP ct-10670 70 36 appetite appetite NOUN ct-10670 70 37 , , PUNCT ct-10670 70 38 feed feed NOUN ct-10670 70 39 intake intake NOUN ct-10670 70 40 , , PUNCT ct-10670 70 41 output output NOUN ct-10670 70 42 of of ADP ct-10670 70 43 feces fece NOUN ct-10670 70 44 and and CCONJ ct-10670 70 45 urine urine NOUN ct-10670 70 46 , , PUNCT ct-10670 70 47 and and CCONJ ct-10670 70 48 signs sign NOUN ct-10670 70 49 of of ADP ct-10670 70 50 discomfort discomfort NOUN ct-10670 70 51 . . PUNCT ct-10670 71 1 as as ADP ct-10670 71 2 a a DET ct-10670 71 3 prophylactic prophylactic ADJ ct-10670 71 4 method method NOUN ct-10670 71 5 , , PUNCT ct-10670 71 6 we we PRON ct-10670 71 7 give give VERB ct-10670 71 8 oral oral ADJ ct-10670 71 9     SPACE ct-10670 71 10 equisul equisul NOUN ct-10670 71 11 ( ( PUNCT ct-10670 71 12 sulfadiazine sulfadiazine NOUN ct-10670 71 13 and and CCONJ ct-10670 71 14 trimethoprim trimethoprim ADJ ct-10670 71 15 , , PUNCT ct-10670 71 16 24 24 NUM ct-10670 71 17 mg mg PROPN ct-10670 71 18 / / SYM ct-10670 71 19 kg kg NOUN ct-10670 71 20 ) ) PUNCT ct-10670 71 21 prior prior ADV ct-10670 71 22 to to ADP ct-10670 71 23 the the DET ct-10670 71 24 procedure procedure NOUN ct-10670 71 25 and and CCONJ ct-10670 71 26 2 2 NUM ct-10670 71 27 extra extra ADJ ct-10670 71 28 doses dose NOUN ct-10670 71 29 every every DET ct-10670 71 30 12 12 NUM ct-10670 71 31 hours hour NOUN ct-10670 71 32 after after ADP ct-10670 71 33 the the DET ct-10670 71 34 procedure procedure NOUN ct-10670 71 35 ( ( PUNCT ct-10670 71 36 total total NOUN ct-10670 71 37 of of ADP ct-10670 71 38 3 3 NUM ct-10670 71 39 doses dose NOUN ct-10670 71 40 ) ) PUNCT ct-10670 71 41 . . PUNCT ct-10670 72 1 we we PRON ct-10670 72 2 also also ADV ct-10670 72 3 use use VERB ct-10670 72 4 flunixin flunixin NOUN ct-10670 72 5 meglumine meglumine NOUN ct-10670 72 6 ( ( PUNCT ct-10670 72 7 banamine banamine PROPN ct-10670 72 8 ® ® NOUN ct-10670 72 9 , , PUNCT ct-10670 72 10 1.1 1.1 NUM ct-10670 72 11 mg mg NUM ct-10670 72 12 / / SYM ct-10670 72 13 kg kg NOUN ct-10670 72 14 ) ) PUNCT ct-10670 72 15 before before ADP ct-10670 72 16 the the DET ct-10670 72 17 procedure procedure NOUN ct-10670 72 18 and and CCONJ ct-10670 72 19 an an DET ct-10670 72 20 extra extra ADJ ct-10670 72 21 dose dose NOUN ct-10670 72 22 12 12 NUM ct-10670 72 23 hours hour NOUN ct-10670 72 24 prior prior ADV ct-10670 72 25 to to ADP ct-10670 72 26 a a DET ct-10670 72 27 mare mare NOUN ct-10670 72 28 ’s ’s PART ct-10670 72 29 discharge discharge NOUN ct-10670 72 30 from from ADP ct-10670 72 31 the the DET ct-10670 72 32 veterinary veterinary ADJ ct-10670 72 33 medical medical ADJ ct-10670 72 34 teaching teaching NOUN ct-10670 72 35 hospital hospital NOUN ct-10670 72 36 . . PUNCT ct-10670 73 1 transvaginal transvaginal ADJ ct-10670 73 2 aspiration aspiration NOUN ct-10670 73 3 of of ADP ct-10670 73 4 oocytes oocyte NOUN ct-10670 73 5 can can AUX ct-10670 73 6 be be AUX ct-10670 73 7 performed perform VERB ct-10670 73 8 by by ADP ct-10670 73 9 a a DET ct-10670 73 10 single single ADJ ct-10670 73 11 highly highly ADV ct-10670 73 12 experienced experienced ADJ ct-10670 73 13 person person NOUN ct-10670 73 14 ; ; PUNCT ct-10670 73 15 but but CCONJ ct-10670 73 16 as as ADP ct-10670 73 17 a a DET ct-10670 73 18 teaching teaching NOUN ct-10670 73 19 hospital hospital NOUN ct-10670 73 20 , , PUNCT ct-10670 73 21 we we PRON ct-10670 73 22 prefer prefer VERB ct-10670 73 23 a a DET ct-10670 73 24 team team NOUN ct-10670 73 25 approach approach NOUN ct-10670 73 26 to to PART ct-10670 73 27 provide provide VERB ct-10670 73 28 training training NOUN ct-10670 73 29 in in ADP ct-10670 73 30 all all DET ct-10670 73 31 aspects aspect NOUN ct-10670 73 32 of of ADP ct-10670 73 33 the the DET ct-10670 73 34 process process NOUN ct-10670 73 35 for for ADP ct-10670 73 36 residents resident NOUN ct-10670 73 37 and and CCONJ ct-10670 73 38 other other ADJ ct-10670 73 39 veterinarians veterinarian NOUN ct-10670 73 40 . . PUNCT ct-10670 74 1 our our PRON ct-10670 74 2 clinicians clinician NOUN ct-10670 74 3 follow follow VERB ct-10670 74 4 an an DET ct-10670 74 5 established establish VERB ct-10670 74 6 protocol protocol NOUN ct-10670 74 7 , , PUNCT ct-10670 74 8 with with ADP ct-10670 74 9 2 2 NUM ct-10670 74 10 veterinarians veterinarian NOUN ct-10670 74 11 operating operate VERB ct-10670 74 12 the the DET ct-10670 74 13 rectal rectal ADJ ct-10670 74 14 and and CCONJ ct-10670 74 15 vaginal vaginal ADJ ct-10670 74 16 part part NOUN ct-10670 74 17 of of ADP ct-10670 74 18 tva tva PROPN ct-10670 74 19 , , PUNCT ct-10670 74 20 respectively respectively ADV ct-10670 74 21 . . PUNCT ct-10670 75 1 first first ADJ ct-10670 75 2 person person NOUN ct-10670 75 3 maneuvers maneuver VERB ct-10670 75 4 the the DET ct-10670 75 5 ovaries ovary NOUN ct-10670 75 6 transrectally transrectally ADV ct-10670 75 7 and and CCONJ ct-10670 75 8 then then ADV ct-10670 75 9 manipulates manipulate VERB ct-10670 75 10 1 1 NUM ct-10670 75 11 ovary ovary NOUN ct-10670 75 12 to to PART ct-10670 75 13 fix fix VERB ct-10670 75 14 it it PRON ct-10670 75 15 against against ADP ct-10670 75 16 the the DET ct-10670 75 17 transvaginal transvaginal ADJ ct-10670 75 18 probe probe NOUN ct-10670 75 19 for for ADP ct-10670 75 20 optimal optimal ADJ ct-10670 75 21 visualization visualization NOUN ct-10670 75 22 . . PUNCT ct-10670 76 1 second second ADJ ct-10670 76 2 person person NOUN ct-10670 76 3 operates operate VERB ct-10670 76 4 the the DET ct-10670 76 5 needle needle NOUN ct-10670 76 6 and and CCONJ ct-10670 76 7 aspiration aspiration NOUN ct-10670 76 8 pump pump NOUN ct-10670 76 9 , , PUNCT ct-10670 76 10 being be AUX ct-10670 76 11 responsible responsible ADJ ct-10670 76 12 for for ADP ct-10670 76 13 the the DET ct-10670 76 14 puncture puncture NOUN ct-10670 76 15 and and CCONJ ct-10670 76 16 scraping scraping NOUN ct-10670 76 17 of of ADP ct-10670 76 18 each each DET ct-10670 76 19 follicle follicle NOUN ct-10670 76 20 ( ( PUNCT ct-10670 76 21 figure figure NOUN ct-10670 76 22 1 1 NUM ct-10670 76 23 ) ) PUNCT ct-10670 76 24 . . PUNCT ct-10670 77 1 the the DET ct-10670 77 2 double double ADJ ct-10670 77 3 - - PUNCT ct-10670 77 4 lumen luman NOUN ct-10670 77 5 needle needle NOUN ct-10670 77 6 allows allow VERB ct-10670 77 7 a a DET ct-10670 77 8 separate separate ADJ ct-10670 77 9 channel channel NOUN ct-10670 77 10 for for ADP ct-10670 77 11 inflow inflow NOUN ct-10670 77 12 and and CCONJ ct-10670 77 13 outflow outflow NOUN ct-10670 77 14 from from ADP ct-10670 77 15 follicles follicle NOUN ct-10670 77 16 . . PUNCT ct-10670 78 1 the the DET ct-10670 78 2 latter latter ADJ ct-10670 78 3 person person NOUN ct-10670 78 4 typically typically ADV ct-10670 78 5 operates operate VERB ct-10670 78 6 the the DET ct-10670 78 7 vacuum vacuum NOUN ct-10670 78 8 pump pump NOUN ct-10670 78 9 for for ADP ct-10670 78 10 follicular follicular ADJ ct-10670 78 11 lavage lavage NOUN ct-10670 78 12 using use VERB ct-10670 78 13 a a DET ct-10670 78 14 foot foot NOUN ct-10670 78 15 pedal pedal NOUN ct-10670 78 16 . . PUNCT ct-10670 79 1 a a DET ct-10670 79 2 third third ADJ ct-10670 79 3 person person NOUN ct-10670 79 4 manually manually ADV ct-10670 79 5 manages manage VERB ct-10670 79 6 the the DET ct-10670 79 7 fluid fluid ADJ ct-10670 79 8 injection injection NOUN ct-10670 79 9 into into ADP ct-10670 79 10 the the DET ct-10670 79 11 punctured punctured ADJ ct-10670 79 12 follicle follicle NOUN ct-10670 79 13 using use VERB ct-10670 79 14 equiplus equiplus PROPN ct-10670 79 15 opu opu PROPN ct-10670 79 16 recovery recovery PROPN ct-10670 79 17 medium medium NOUN ct-10670 79 18 ( ( PUNCT ct-10670 79 19 minitube minitube NOUN ct-10670 79 20 ) ) PUNCT ct-10670 79 21 . . PUNCT ct-10670 80 1 all all DET ct-10670 80 2 procedures procedure NOUN ct-10670 80 3 are be AUX ct-10670 80 4 under under ADP ct-10670 80 5 strict strict ADJ ct-10670 80 6 aseptic aseptic ADJ ct-10670 80 7 conditions condition NOUN ct-10670 80 8 with with ADP ct-10670 80 9 care care NOUN ct-10670 80 10 to to PART ct-10670 80 11 maintain maintain VERB ct-10670 80 12 them they PRON ct-10670 80 13 at at ADP ct-10670 80 14 all all DET ct-10670 80 15 times time NOUN ct-10670 80 16 . . PUNCT ct-10670 81 1 typically typically ADV ct-10670 81 2 , , PUNCT ct-10670 81 3 5 5 NUM ct-10670 81 4 - - SYM ct-10670 81 5 20 20 NUM ct-10670 81 6 oocytes oocyte NOUN ct-10670 81 7 are be AUX ct-10670 81 8 retrieved retrieve VERB ct-10670 81 9 per per ADP ct-10670 81 10 tva tva PROPN ct-10670 81 11 session session NOUN ct-10670 81 12 ( ( PUNCT ct-10670 81 13 12 12 NUM ct-10670 81 14 ± ± NUM ct-10670 81 15 7 7 NUM ct-10670 82 1 [ [ X ct-10670 82 2 average average ADJ ct-10670 82 3 ± ± NUM ct-10670 82 4 sd sd NOUN ct-10670 82 5 ] ] PUNCT ct-10670 82 6 ; ; PUNCT ct-10670 82 7 n n PROPN ct-10670 82 8 = = SYM ct-10670 82 9 136 136 NUM ct-10670 82 10 ) ) PUNCT ct-10670 82 11 . . PUNCT ct-10670 83 1 in in ADP ct-10670 83 2 the the DET ct-10670 83 3 case case NOUN ct-10670 83 4 of of ADP ct-10670 83 5 a a DET ct-10670 83 6 single single ADJ ct-10670 83 7 mature mature ADJ ct-10670 83 8 preovulatory preovulatory ADJ ct-10670 83 9 follicle follicle NOUN ct-10670 83 10 ( ( PUNCT ct-10670 83 11 > > X ct-10670 83 12 35 35 NUM ct-10670 83 13 mm mm NOUN ct-10670 83 14 ) ) PUNCT ct-10670 83 15 present present NOUN ct-10670 83 16 on on ADP ct-10670 83 17 1 1 NUM ct-10670 83 18 or or CCONJ ct-10670 83 19 both both PRON ct-10670 83 20 ovaries ovary NOUN ct-10670 83 21 , , PUNCT ct-10670 83 22 we we PRON ct-10670 83 23 will will AUX ct-10670 83 24 aspirate aspirate VERB ct-10670 83 25 that that DET ct-10670 83 26 follicle follicle NOUN ct-10670 83 27 and and CCONJ ct-10670 83 28 search search VERB ct-10670 83 29 for for ADP ct-10670 83 30 an an DET ct-10670 83 31 oocyte oocyte NOUN ct-10670 83 32 . . PUNCT ct-10670 84 1 since since SCONJ ct-10670 84 2 such such DET ct-10670 84 3 an an DET ct-10670 84 4 oocyte oocyte NOUN ct-10670 84 5 has have AUX ct-10670 84 6 likely likely ADV ct-10670 84 7 already already ADV ct-10670 84 8 started start VERB ct-10670 84 9 maturation maturation NOUN ct-10670 84 10 in in ADP ct-10670 84 11 vivo vivo NOUN ct-10670 84 12 , , PUNCT ct-10670 84 13 they they PRON ct-10670 84 14 generally generally ADV ct-10670 84 15 have have VERB ct-10670 84 16 a a DET ct-10670 84 17 higher high ADJ ct-10670 84 18 developmental developmental ADJ ct-10670 84 19 potential potential ADJ ct-10670 84 20 figure figure NOUN ct-10670 84 21 1 1 NUM ct-10670 84 22 . . PUNCT ct-10670 84 23 illustration illustration NOUN ct-10670 84 24 of of ADP ct-10670 84 25 a a DET ct-10670 84 26 mare mare NOUN ct-10670 84 27 ’s ’s PART ct-10670 84 28 ovary ovary NOUN ct-10670 84 29 having have VERB ct-10670 84 30 2 2 NUM ct-10670 84 31 immature immature ADJ ct-10670 84 32 antral antral ADJ ct-10670 84 33 follicles follicle NOUN ct-10670 84 34 in in ADP ct-10670 84 35 addition addition NOUN ct-10670 84 36 to to ADP ct-10670 84 37 a a DET ct-10670 84 38 corpus corpus NOUN ct-10670 84 39 luteum luteum NOUN ct-10670 84 40 . . PUNCT ct-10670 85 1 an an DET ct-10670 85 2 aspiration aspiration NOUN ct-10670 85 3 needle needle NOUN ct-10670 85 4 tip tip NOUN ct-10670 85 5 is be AUX ct-10670 85 6 depicted depict VERB ct-10670 85 7 within within ADP ct-10670 85 8 the the DET ct-10670 85 9 larger large ADJ ct-10670 85 10 follicle follicle NOUN ct-10670 85 11 during during ADP ct-10670 85 12 aspiration aspiration NOUN ct-10670 85 13 and and CCONJ ct-10670 85 14 scraping scraping NOUN ct-10670 85 15 ( ( PUNCT ct-10670 85 16 nonscale nonscale ADJ ct-10670 85 17 representation representation NOUN ct-10670 85 18 ) ) PUNCT ct-10670 85 19 . . PUNCT ct-10670 86 1 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 X ct-10670 86 2 4 4 NUM ct-10670 86 3 citation citation NOUN ct-10670 86 4 : : PUNCT ct-10670 86 5 clinical clinical ADJ ct-10670 86 6 theriogenology theriogenology NOUN ct-10670 86 7 2024 2024 NUM ct-10670 86 8 , , PUNCT ct-10670 86 9 16 16 NUM ct-10670 86 10 , , PUNCT ct-10670 86 11 10670 10670 NUM ct-10670 86 12 , , PUNCT ct-10670 86 13 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 X ct-10670 86 14 with with ADP ct-10670 86 15 icsi icsi ADJ ct-10670 86 16 injection injection NOUN ct-10670 86 17 of of ADP ct-10670 86 18 sperm sperm NOUN ct-10670 86 19 , , PUNCT ct-10670 86 20 but but CCONJ ct-10670 86 21 the the DET ct-10670 86 22 blastocyst blastocyst NOUN ct-10670 86 23 yield yield NOUN ct-10670 86 24 per per ADP ct-10670 86 25 mare mare NOUN ct-10670 86 26 is be AUX ct-10670 86 27 slightly slightly ADV ct-10670 86 28 lower low ADJ ct-10670 86 29 . . PUNCT ct-10670 87 1 after after ADP ct-10670 87 2 tva tva PROPN ct-10670 87 3 , , PUNCT ct-10670 87 4 the the DET ct-10670 87 5 aspirated aspirated ADJ ct-10670 87 6 fluid fluid NOUN ct-10670 87 7 is be AUX ct-10670 87 8 transported transport VERB ct-10670 87 9 to to ADP ct-10670 87 10 laboratory laboratory NOUN ct-10670 87 11 at at ADP ct-10670 87 12 ambient ambient ADJ ct-10670 87 13 temperature temperature NOUN ct-10670 87 14 , , PUNCT ct-10670 87 15 filtered filter VERB ct-10670 87 16 using use VERB ct-10670 87 17 opu opu PROPN ct-10670 87 18 recovery recovery PROPN ct-10670 87 19 medium medium NOUN ct-10670 87 20 and and CCONJ ct-10670 87 21 an an DET ct-10670 87 22 emcon emcon ADJ ct-10670 87 23 embryo embryo NOUN ct-10670 87 24 filter filter NOUN ct-10670 87 25 that that PRON ct-10670 87 26 is be AUX ct-10670 87 27 carefully carefully ADV ct-10670 87 28 rinsed rinse VERB ct-10670 87 29 and and CCONJ ct-10670 87 30 flushed flush VERB ct-10670 87 31 into into ADP ct-10670 87 32 large large ADJ ct-10670 87 33 petri petri ADJ ct-10670 87 34 dishes dish NOUN ct-10670 87 35 which which PRON ct-10670 87 36 are be AUX ct-10670 87 37 then then ADV ct-10670 87 38 searched search VERB ct-10670 87 39 using use VERB ct-10670 87 40 a a DET ct-10670 87 41 bright bright ADJ ct-10670 87 42 - - PUNCT ct-10670 87 43 field field NOUN ct-10670 87 44 stereomicroscope stereomicroscope NOUN ct-10670 87 45 . . PUNCT ct-10670 88 1 identified identify VERB ct-10670 88 2 oocytes oocyte NOUN ct-10670 88 3 are be AUX ct-10670 88 4 then then ADV ct-10670 88 5 placed place VERB ct-10670 88 6 into into ADP ct-10670 88 7 holding hold VERB ct-10670 88 8 medium medium NOUN ct-10670 88 9 ( ( PUNCT ct-10670 88 10 see see VERB ct-10670 88 11 below below ADV ct-10670 88 12 ) ) PUNCT ct-10670 88 13 overnight overnight ADV ct-10670 88 14 ( ( PUNCT ct-10670 88 15 12 12 NUM ct-10670 88 16 - - SYM ct-10670 88 17 16 16 NUM ct-10670 88 18 hours hour NOUN ct-10670 88 19 ) ) PUNCT ct-10670 88 20 in in ADP ct-10670 88 21 sterile sterile ADJ ct-10670 88 22 glass glass NOUN ct-10670 88 23 vials vial NOUN ct-10670 88 24 at at ADP ct-10670 88 25 ambient ambient ADJ ct-10670 88 26 temperature temperature NOUN ct-10670 88 27 ( ( PUNCT ct-10670 88 28 22oc 22oc ADJ ct-10670 88 29 ) ) PUNCT ct-10670 88 30 until until SCONJ ct-10670 88 31 they they PRON ct-10670 88 32 are be AUX ct-10670 88 33 placed place VERB ct-10670 88 34 into into ADP ct-10670 88 35 maturation maturation NOUN ct-10670 88 36 medium medium NOUN ct-10670 88 37 in in ADP ct-10670 88 38 a a DET ct-10670 88 39 trigas trigas NOUN ct-10670 88 40 environment environment NOUN ct-10670 88 41 for for ADP ct-10670 88 42 27 27 NUM ct-10670 88 43 - - SYM ct-10670 88 44 30 30 NUM ct-10670 88 45 hours hour NOUN ct-10670 88 46 in in ADP ct-10670 88 47 droplets droplet NOUN ct-10670 88 48 of of ADP ct-10670 88 49 maturation maturation NOUN ct-10670 88 50 medium medium NOUN ct-10670 88 51 at at ADP ct-10670 88 52 a a DET ct-10670 88 53 ratio ratio NOUN ct-10670 88 54 of of ADP ct-10670 88 55 10 10 NUM ct-10670 88 56 µl µl NOUN ct-10670 88 57 per per ADP ct-10670 88 58 oocyte oocyte NOUN ct-10670 88 59 under under ADP ct-10670 88 60 light light ADJ ct-10670 88 61 paraffin paraffin NOUN ct-10670 88 62 oil oil NOUN ct-10670 88 63 at at ADP ct-10670 88 64 38.2oc 38.2oc NUM ct-10670 88 65 in in ADP ct-10670 88 66 a a DET ct-10670 88 67 humidified humidify VERB ct-10670 88 68 atmosphere atmosphere NOUN ct-10670 88 69 of of ADP ct-10670 88 70 5.8 5.8 NUM ct-10670 88 71 % % NOUN ct-10670 88 72 co2 co2 NOUN ct-10670 88 73 , , PUNCT ct-10670 88 74 5%o2 5%o2 NUM ct-10670 88 75 , , PUNCT ct-10670 88 76 and and CCONJ ct-10670 88 77 89.2 89.2 NUM ct-10670 88 78 % % NOUN ct-10670 88 79 n2 n2 NOUN ct-10670 88 80 . . PUNCT ct-10670 89 1 oocytes oocyte NOUN ct-10670 89 2 are be AUX ct-10670 89 3 then then ADV ct-10670 89 4 denuded denude VERB ct-10670 89 5 from from ADP ct-10670 89 6 cumulus cumulus NOUN ct-10670 89 7 cells cell NOUN ct-10670 89 8 and and CCONJ ct-10670 89 9 evaluated evaluate VERB ct-10670 89 10 for for ADP ct-10670 89 11 extrusion extrusion NOUN ct-10670 89 12 of of ADP ct-10670 89 13 the the DET ct-10670 89 14 first first ADJ ct-10670 89 15 polar polar ADJ ct-10670 89 16 body body NOUN ct-10670 89 17 into into ADP ct-10670 89 18 the the DET ct-10670 89 19 perivitelline perivitelline ADJ ct-10670 89 20 space space NOUN ct-10670 89 21 , , PUNCT ct-10670 89 22 indicating indicate VERB ct-10670 89 23 that that SCONJ ct-10670 89 24 prophase prophase PROPN ct-10670 89 25 ii ii NOUN ct-10670 89 26 of of ADP ct-10670 89 27 meiosis meiosis NOUN ct-10670 89 28 has have AUX ct-10670 89 29 reached reach VERB ct-10670 89 30 and and CCONJ ct-10670 89 31 oocytes oocyte NOUN ct-10670 89 32 are be AUX ct-10670 89 33 ready ready ADJ ct-10670 89 34 for for ADP ct-10670 89 35 fertilization fertilization NOUN ct-10670 89 36 by by ADP ct-10670 89 37 sperm sperm NOUN ct-10670 89 38 microinjection microinjection NOUN ct-10670 89 39 . . PUNCT ct-10670 90 1 oocyte oocyte ADJ ct-10670 90 2 transport transport NOUN ct-10670 90 3 typically typically ADV ct-10670 90 4 , , PUNCT ct-10670 90 5 > > X ct-10670 90 6 80 80 NUM ct-10670 90 7 % % NOUN ct-10670 90 8 of of ADP ct-10670 90 9 oocytes oocyte NOUN ct-10670 90 10 used use VERB ct-10670 90 11 for for ADP ct-10670 90 12 icsi icsi NOUN ct-10670 90 13 are be AUX ct-10670 90 14 shipped ship VERB ct-10670 90 15 to to ADP ct-10670 90 16 our our PRON ct-10670 90 17 facility facility NOUN ct-10670 90 18 from from ADP ct-10670 90 19 veterinarians veterinarian NOUN ct-10670 90 20 in in ADP ct-10670 90 21 the the DET ct-10670 90 22 us us PROPN ct-10670 90 23 . . PUNCT ct-10670 91 1 our our PRON ct-10670 91 2 laboratory laboratory NOUN ct-10670 91 3 receives receive VERB ct-10670 91 4 oocytes oocyte NOUN ct-10670 91 5 in in ADP ct-10670 91 6 sterile sterile ADJ ct-10670 91 7 glass glass NOUN ct-10670 91 8 vials vial NOUN ct-10670 91 9 in in ADP ct-10670 91 10 1 1 NUM ct-10670 91 11 or or CCONJ ct-10670 91 12 3 3 NUM ct-10670 91 13 ml ml NOUN ct-10670 91 14 volume volume NOUN ct-10670 91 15 of of ADP ct-10670 91 16 emcare emcare PROPN ct-10670 91 17 ( ( PUNCT ct-10670 91 18 icpbio icpbio NOUN ct-10670 91 19 reproduction reproduction NOUN ct-10670 91 20 , , PUNCT ct-10670 91 21 spring spring NOUN ct-10670 91 22 valley valley NOUN ct-10670 91 23 , , PUNCT ct-10670 91 24 wi wi PROPN ct-10670 91 25 ) ) PUNCT ct-10670 91 26 or or CCONJ ct-10670 91 27 equihold equihold NOUN ct-10670 91 28 ( ( PUNCT ct-10670 91 29 minitube minitube PROPN ct-10670 91 30 , , PUNCT ct-10670 91 31 verona verona PROPN ct-10670 91 32 , , PUNCT ct-10670 91 33 wi wi PROPN ct-10670 91 34 ) ) PUNCT ct-10670 91 35 commercial commercial ADJ ct-10670 91 36 media medium NOUN ct-10670 91 37 , , PUNCT ct-10670 91 38 as as SCONJ ct-10670 91 39 they they PRON ct-10670 91 40 are be AUX ct-10670 91 41 available available ADJ ct-10670 91 42 to to ADP ct-10670 91 43 most most ADJ ct-10670 91 44 clinics clinic NOUN ct-10670 91 45 shipping shipping NOUN ct-10670 91 46 oocytes oocyte NOUN ct-10670 91 47 , , PUNCT ct-10670 91 48 and and CCONJ ct-10670 91 49 oocytes oocyte NOUN ct-10670 91 50 are be AUX ct-10670 91 51 stable stable ADJ ct-10670 91 52 in in ADP ct-10670 91 53 these these DET ct-10670 91 54 media medium NOUN ct-10670 91 55 at at ADP ct-10670 91 56 ambient ambient ADJ ct-10670 91 57 temperature temperature NOUN ct-10670 91 58 for for ADP ct-10670 91 59 at at ADV ct-10670 91 60 least least ADJ ct-10670 91 61 24 24 NUM ct-10670 91 62 hours.8,11 hours.8,11 NOUN ct-10670 91 63 oocytes oocyte NOUN ct-10670 91 64 are be AUX ct-10670 91 65 express express ADV ct-10670 91 66 - - PUNCT ct-10670 91 67 shipped ship VERB ct-10670 91 68 overnight overnight ADV ct-10670 91 69 in in ADP ct-10670 91 70 holding hold VERB ct-10670 91 71 medium medium NOUN ct-10670 91 72 with with ADP ct-10670 91 73 minimal minimal ADJ ct-10670 91 74 air air NOUN ct-10670 91 75 in in ADP ct-10670 91 76 either either CCONJ ct-10670 91 77 a a DET ct-10670 91 78 battery battery NOUN ct-10670 91 79 - - PUNCT ct-10670 91 80 powered power VERB ct-10670 91 81 shipping shipping NOUN ct-10670 91 82 incubator incubator NOUN ct-10670 91 83 ( ( PUNCT ct-10670 91 84 microq microq PROPN ct-10670 91 85 technologies technology NOUN ct-10670 91 86 , , PUNCT ct-10670 91 87 mesa mesa PROPN ct-10670 91 88 , , PUNCT ct-10670 91 89 az az PROPN ct-10670 91 90 85204 85204 NUM ct-10670 91 91 ) ) PUNCT ct-10670 91 92 or or CCONJ ct-10670 91 93 in in ADP ct-10670 91 94 the the DET ct-10670 91 95 hamilton hamilton PROPN ct-10670 91 96 biovet biovet PROPN ct-10670 91 97 equitainer equitainer NOUN ct-10670 91 98 or or CCONJ ct-10670 91 99 equocyte equocyte NOUN ct-10670 91 100 shippers shipper NOUN ct-10670 91 101 ( ( PUNCT ct-10670 91 102 ipswich ipswich PROPN ct-10670 91 103 , , PUNCT ct-10670 91 104 ma ma PROPN ct-10670 91 105 ) ) PUNCT ct-10670 91 106 using use VERB ct-10670 91 107 22oc 22oc ADJ ct-10670 91 108 insert insert ADJ ct-10670 91 109 cans can NOUN ct-10670 91 110 ( ( PUNCT ct-10670 91 111 figure figure NOUN ct-10670 91 112 2 2 NUM ct-10670 91 113 ) ) PUNCT ct-10670 91 114 . . PUNCT ct-10670 92 1 generally generally ADV ct-10670 92 2 speaking speak VERB ct-10670 92 3 , , PUNCT ct-10670 92 4 isothermal isothermal ADJ ct-10670 92 5 devices device NOUN ct-10670 92 6 maintain maintain VERB ct-10670 92 7 stable stable ADJ ct-10670 92 8 temperature temperature NOUN ct-10670 92 9 that that PRON ct-10670 92 10 is be AUX ct-10670 92 11 critical critical ADJ ct-10670 92 12 to to ADP ct-10670 92 13 oocyte oocyte PROPN ct-10670 92 14 ’s ’s PART ct-10670 92 15 survival survival NOUN ct-10670 92 16 and and CCONJ ct-10670 92 17 further further ADJ ct-10670 92 18 developmental developmental ADJ ct-10670 92 19 competence competence NOUN ct-10670 92 20 . . PUNCT ct-10670 93 1 we we PRON ct-10670 93 2 recently recently ADV ct-10670 93 3 observed observe VERB ct-10670 93 4 that that SCONJ ct-10670 93 5 microq microq NOUN ct-10670 93 6 and and CCONJ ct-10670 93 7 equitainer equitainer NOUN ct-10670 93 8 supported support VERB ct-10670 93 9 oocyte oocyte ADJ ct-10670 93 10 stability stability NOUN ct-10670 93 11 during during ADP ct-10670 93 12 shipping shipping NOUN ct-10670 93 13 and and CCONJ ct-10670 93 14 their their PRON ct-10670 93 15 subsequent subsequent ADJ ct-10670 93 16 maturation maturation NOUN ct-10670 93 17 , , PUNCT ct-10670 93 18 cleavage cleavage NOUN ct-10670 93 19 , , PUNCT ct-10670 93 20 and and CCONJ ct-10670 93 21 blastocyst blastocyst NOUN ct-10670 93 22 rates rate NOUN ct-10670 93 23 , , PUNCT ct-10670 93 24 comparing compare VERB ct-10670 93 25 them they PRON ct-10670 93 26 to to ADP ct-10670 93 27 those those PRON ct-10670 93 28 held hold VERB ct-10670 93 29 overnight overnight ADV ct-10670 93 30 in in ADP ct-10670 93 31 the the DET ct-10670 93 32 laboratory laboratory NOUN ct-10670 93 33 in in ADP ct-10670 93 34 a a DET ct-10670 93 35 battery battery NOUN ct-10670 93 36 - - PUNCT ct-10670 93 37 powered power VERB ct-10670 93 38 incubator incubator NOUN ct-10670 93 39 . . PUNCT ct-10670 94 1 interestingly interestingly ADV ct-10670 94 2 , , PUNCT ct-10670 94 3 the the DET ct-10670 94 4 overall overall ADJ ct-10670 94 5 blastocyst blastocyst NOUN ct-10670 94 6 rates rate NOUN ct-10670 94 7 were be AUX ct-10670 94 8 consistent consistent ADJ ct-10670 94 9 across across ADP ct-10670 94 10 all all DET ct-10670 94 11 devices device NOUN ct-10670 94 12 , , PUNCT ct-10670 94 13 but but CCONJ ct-10670 94 14 oocytes oocyte NOUN ct-10670 94 15 transported transport VERB ct-10670 94 16 in in ADP ct-10670 94 17 equocyte equocyte NOUN ct-10670 94 18 device device NOUN ct-10670 94 19 had have VERB ct-10670 94 20 a a DET ct-10670 94 21 lower low ADJ ct-10670 94 22 maturation maturation NOUN ct-10670 94 23 rate.22 rate.22 NOUN ct-10670 94 24 cryopreservation cryopreservation NOUN ct-10670 94 25 of of ADP ct-10670 94 26 oocytes oocyte NOUN ct-10670 94 27 remains remain VERB ct-10670 94 28 an an DET ct-10670 94 29 active active ADJ ct-10670 94 30 research research NOUN ct-10670 94 31 area area NOUN ct-10670 94 32 for for ADP ct-10670 94 33 several several ADJ ct-10670 94 34 laboratories laboratory NOUN ct-10670 94 35 ; ; PUNCT ct-10670 94 36 however however ADV ct-10670 94 37 , , PUNCT ct-10670 94 38 vitrification vitrification NOUN ct-10670 94 39 of of ADP ct-10670 94 40 equine equine NOUN ct-10670 94 41 oocytes23–25 oocytes23–25 CCONJ ct-10670 94 42 has have AUX ct-10670 94 43 not not PART ct-10670 94 44 yet yet ADV ct-10670 94 45 yielded yield VERB ct-10670 94 46 commercially commercially ADV ct-10670 94 47 acceptable acceptable ADJ ct-10670 94 48 results result NOUN ct-10670 94 49 . . PUNCT ct-10670 95 1 regardless regardless ADV ct-10670 95 2 , , PUNCT ct-10670 95 3 viable viable ADJ ct-10670 95 4 offspring offspring NOUN ct-10670 95 5 from from ADP ct-10670 95 6 such such DET ct-10670 95 7 a a DET ct-10670 95 8 pregnancy pregnancy NOUN ct-10670 95 9 was be AUX ct-10670 95 10 reported report VERB ct-10670 95 11 in in ADP ct-10670 95 12 2018.26 2018.26 NUM ct-10670 95 13 sperm sperm NOUN ct-10670 95 14 handling handle VERB ct-10670 95 15 nearly nearly ADV ct-10670 95 16 all all PRON ct-10670 95 17 icsi icsi ADJ ct-10670 95 18 sessions session NOUN ct-10670 95 19 at at ADP ct-10670 95 20 our our PRON ct-10670 95 21 laboratory laboratory NOUN ct-10670 95 22 are be AUX ct-10670 95 23 performed perform VERB ct-10670 95 24 with with ADP ct-10670 95 25 frozen frozen ADJ ct-10670 95 26 / / SYM ct-10670 95 27 thawed thaw VERB ct-10670 95 28 sperm sperm NOUN ct-10670 95 29 . . PUNCT ct-10670 96 1 although although SCONJ ct-10670 96 2 full full ADJ ct-10670 96 3 motility motility NOUN ct-10670 96 4 can can AUX ct-10670 96 5 not not PART ct-10670 96 6 be be AUX ct-10670 96 7 restored restore VERB ct-10670 96 8 from from ADP ct-10670 96 9 cryopreserved cryopreserved ADJ ct-10670 96 10 sperm sperm NOUN ct-10670 96 11 , , PUNCT ct-10670 96 12 this this DET ct-10670 96 13 technique technique NOUN ct-10670 96 14 requires require VERB ct-10670 96 15 a a DET ct-10670 96 16 very very ADV ct-10670 96 17 small small ADJ ct-10670 96 18 amount amount NOUN ct-10670 96 19 of of ADP ct-10670 96 20 thawed thaw VERB ct-10670 96 21 sperm sperm NOUN ct-10670 96 22 for for ADP ct-10670 96 23 successful successful ADJ ct-10670 96 24 fertilization fertilization NOUN ct-10670 96 25 of of ADP ct-10670 96 26 all all DET ct-10670 96 27 oocytes oocyte NOUN ct-10670 96 28 . . PUNCT ct-10670 97 1 this this PRON ct-10670 97 2 is be AUX ct-10670 97 3 especially especially ADV ct-10670 97 4 important important ADJ ct-10670 97 5 in in ADP ct-10670 97 6 cases case NOUN ct-10670 97 7 where where SCONJ ct-10670 97 8 issues issue NOUN ct-10670 97 9 with with ADP ct-10670 97 10 collection collection NOUN ct-10670 97 11 or or CCONJ ct-10670 97 12 consistent consistent ADJ ct-10670 97 13 low low ADJ ct-10670 97 14 - - PUNCT ct-10670 97 15 quality quality NOUN ct-10670 97 16 sperm sperm NOUN ct-10670 97 17 occur occur VERB ct-10670 97 18 , , PUNCT ct-10670 97 19 and and CCONJ ct-10670 97 20 when when SCONJ ct-10670 97 21 limited limited ADJ ct-10670 97 22 numbers number NOUN ct-10670 97 23 of of ADP ct-10670 97 24 straws straw NOUN ct-10670 97 25 are be AUX ct-10670 97 26 available available ADJ ct-10670 97 27 ( ( PUNCT ct-10670 97 28 i.e. i.e. X ct-10670 97 29 from from ADP ct-10670 97 30 deceased deceased ADJ ct-10670 97 31 or or CCONJ ct-10670 97 32 castrated castrate VERB ct-10670 97 33 stallions stallion NOUN ct-10670 97 34 ) ) PUNCT ct-10670 97 35 . . PUNCT ct-10670 98 1 in in ADP ct-10670 98 2 our our PRON ct-10670 98 3 observations observation NOUN ct-10670 98 4 , , PUNCT ct-10670 98 5 some some DET ct-10670 98 6 stallions stallion NOUN ct-10670 98 7 perform perform VERB ct-10670 98 8 better well ADV ct-10670 98 9 than than ADP ct-10670 98 10 others other NOUN ct-10670 98 11 , , PUNCT ct-10670 98 12 having have VERB ct-10670 98 13 higher high ADJ ct-10670 98 14 fertilization fertilization NOUN ct-10670 98 15 , , PUNCT ct-10670 98 16 cleavage cleavage NOUN ct-10670 98 17 , , PUNCT ct-10670 98 18 and and CCONJ ct-10670 98 19 blastocyst blastocyst NOUN ct-10670 98 20 rates rate NOUN ct-10670 98 21 after after ADP ct-10670 98 22 icsi icsi ADJ ct-10670 98 23 sessions session NOUN ct-10670 98 24 . . PUNCT ct-10670 99 1 furthermore furthermore ADV ct-10670 99 2 , , PUNCT ct-10670 99 3 certain certain ADJ ct-10670 99 4 mares mare NOUN ct-10670 99 5 paired pair VERB ct-10670 99 6 with with ADP ct-10670 99 7 specific specific ADJ ct-10670 99 8 stallions stallion NOUN ct-10670 99 9 achieve achieve VERB ct-10670 99 10 highly highly ADV ct-10670 99 11 variable variable ADJ ct-10670 99 12 blastocyst blastocyst NOUN ct-10670 99 13 rates rate NOUN ct-10670 99 14 , , PUNCT ct-10670 99 15 depending depend VERB ct-10670 99 16 on on ADP ct-10670 99 17 the the DET ct-10670 99 18 specific specific ADJ ct-10670 99 19 breeding breeding NOUN ct-10670 99 20 cross cross NOUN ct-10670 99 21 . . PUNCT ct-10670 100 1 similarly similarly ADV ct-10670 100 2 , , PUNCT ct-10670 100 3 different different ADJ ct-10670 100 4 batches batch NOUN ct-10670 100 5 of of ADP ct-10670 100 6 cryopreserved cryopreserved ADJ ct-10670 100 7 semen semen NOUN ct-10670 100 8 from from ADP ct-10670 100 9 the the DET ct-10670 100 10 same same ADJ ct-10670 100 11 stallion stallion NOUN ct-10670 100 12 can can AUX ct-10670 100 13 be be AUX ct-10670 100 14 of of ADP ct-10670 100 15 dramatically dramatically ADV ct-10670 100 16 different different ADJ ct-10670 100 17 quality quality NOUN ct-10670 100 18 . . PUNCT ct-10670 101 1 typically typically ADV ct-10670 101 2 , , PUNCT ct-10670 101 3 when when SCONJ ct-10670 101 4 possible possible ADJ ct-10670 101 5 , , PUNCT ct-10670 101 6 we we PRON ct-10670 101 7 request request VERB ct-10670 101 8 batches batch NOUN ct-10670 101 9 of of ADP ct-10670 101 10 frozen frozen ADJ ct-10670 101 11 semen semen NOUN ct-10670 101 12 from from ADP ct-10670 101 13 stallion stallion NOUN ct-10670 101 14 management management NOUN ct-10670 101 15 that that PRON ct-10670 101 16 have have AUX ct-10670 101 17 known know VERB ct-10670 101 18 icsi icsi ADJ ct-10670 101 19 success success NOUN ct-10670 101 20 rates rate NOUN ct-10670 101 21 . . PUNCT ct-10670 102 1 our our PRON ct-10670 102 2 laboratory laboratory NOUN ct-10670 102 3 maintains maintain VERB ct-10670 102 4 a a DET ct-10670 102 5 commercial commercial ADJ ct-10670 102 6 sperm sperm NOUN ct-10670 102 7 bank bank NOUN ct-10670 102 8 from from ADP ct-10670 102 9 highly highly ADV ct-10670 102 10 desired desire VERB ct-10670 102 11 sires sire NOUN ct-10670 102 12 for for ADP ct-10670 102 13 the the DET ct-10670 102 14 mare mare NOUN ct-10670 102 15 clients client NOUN ct-10670 102 16 ’ ’ PART ct-10670 102 17 convenience convenience NOUN ct-10670 102 18 , , PUNCT ct-10670 102 19 but but CCONJ ct-10670 102 20 every every DET ct-10670 102 21 icsi icsi ADJ ct-10670 102 22 cycle cycle NOUN ct-10670 102 23 requires require VERB ct-10670 102 24 legally legally ADV ct-10670 102 25 binding bind VERB ct-10670 102 26 contracts contract NOUN ct-10670 102 27 . . PUNCT ct-10670 103 1 our our PRON ct-10670 103 2 optimized optimize VERB ct-10670 103 3 technique technique NOUN ct-10670 103 4 allows allow VERB ct-10670 103 5 us we PRON ct-10670 103 6 to to PART ct-10670 103 7 perform perform VERB ct-10670 103 8 straw straw NOUN ct-10670 103 9 cuts cut NOUN ct-10670 103 10 such such ADJ ct-10670 103 11 that that SCONJ ct-10670 103 12 only only ADV ct-10670 103 13 1/12 1/12 NUM ct-10670 103 14 to to PART ct-10670 103 15 1/8 1/8 NUM ct-10670 103 16 of of ADP ct-10670 103 17 a a DET ct-10670 103 18 straw straw NOUN ct-10670 103 19 is be AUX ct-10670 103 20 used use VERB ct-10670 103 21 in in ADP ct-10670 103 22 each each DET ct-10670 103 23 session session NOUN ct-10670 103 24 , , PUNCT ct-10670 103 25 conserving conserve VERB ct-10670 103 26 semen semen NOUN ct-10670 103 27 samples sample NOUN ct-10670 103 28 that that PRON ct-10670 103 29 oftentimes oftentime NOUN ct-10670 103 30 are be AUX ct-10670 103 31 limited limited ADJ ct-10670 103 32 and and CCONJ ct-10670 103 33 highly highly ADV ct-10670 103 34 valuable valuable ADJ ct-10670 103 35 . . PUNCT ct-10670 104 1 for for ADP ct-10670 104 2 the the DET ct-10670 104 3 same same ADJ ct-10670 104 4 reason reason NOUN ct-10670 104 5 , , PUNCT ct-10670 104 6 we we PRON ct-10670 104 7 try try VERB ct-10670 104 8 to to PART ct-10670 104 9 schedule schedule VERB ct-10670 104 10 oocyte oocyte ADJ ct-10670 104 11 collections collection NOUN ct-10670 104 12 on on ADP ct-10670 104 13 the the DET ct-10670 104 14 same same ADJ ct-10670 104 15 day day NOUN ct-10670 104 16 so so SCONJ ct-10670 104 17 they they PRON ct-10670 104 18 can can AUX ct-10670 104 19 be be AUX ct-10670 104 20 fertilized fertilize VERB ct-10670 104 21 with with ADP ct-10670 104 22 sperm sperm NOUN ct-10670 104 23 from from ADP ct-10670 104 24 a a DET ct-10670 104 25 single single ADJ ct-10670 104 26 straw straw NOUN ct-10670 104 27 cut cut NOUN ct-10670 104 28 . . PUNCT ct-10670 105 1 the the DET ct-10670 105 2 cutting cutting NOUN ct-10670 105 3 is be AUX ct-10670 105 4 performed perform VERB ct-10670 105 5 under under ADP ct-10670 105 6 liquid liquid ADJ ct-10670 105 7 nitrogen nitrogen NOUN ct-10670 105 8 to to PART ct-10670 105 9 prevent prevent VERB ct-10670 105 10 thawing thawing NOUN ct-10670 105 11 of of ADP ct-10670 105 12 the the DET ct-10670 105 13 remaining remain VERB ct-10670 105 14 straw straw NOUN ct-10670 105 15 segment segment NOUN ct-10670 105 16 . . PUNCT ct-10670 106 1 in in ADP ct-10670 106 2 our our PRON ct-10670 106 3 laboratory laboratory NOUN ct-10670 106 4 , , PUNCT ct-10670 106 5 we we PRON ct-10670 106 6 perform perform VERB ct-10670 106 7 swim swim NOUN ct-10670 106 8 - - PUNCT ct-10670 106 9 up up NOUN ct-10670 106 10 as as ADP ct-10670 106 11 the the DET ct-10670 106 12 selection selection NOUN ct-10670 106 13 method method NOUN ct-10670 106 14 for for ADP ct-10670 106 15 high high ADJ ct-10670 106 16 - - PUNCT ct-10670 106 17 quality quality NOUN ct-10670 106 18 sperm sperm NOUN ct-10670 106 19 . . PUNCT ct-10670 107 1 swim swim NOUN ct-10670 107 2 - - PUNCT ct-10670 107 3 up up NOUN ct-10670 107 4 is be AUX ct-10670 107 5 performed perform VERB ct-10670 107 6 by by ADP ct-10670 107 7 layering layer VERB ct-10670 107 8 the the DET ct-10670 107 9 thawed thawed ADJ ct-10670 107 10 sample sample NOUN ct-10670 107 11 underneath underneath ADP ct-10670 107 12 2.0 2.0 NUM ct-10670 107 13 ml ml NOUN ct-10670 107 14 g g NOUN ct-10670 107 15 - - PUNCT ct-10670 107 16 mops mop NOUN ct-10670 107 17 ( ( PUNCT ct-10670 107 18 vitrolife vitrolife NOUN ct-10670 107 19 , , PUNCT ct-10670 107 20 inc inc PROPN ct-10670 107 21 . . PROPN ct-10670 107 22 sweden sweden PROPN ct-10670 107 23 ) ) PUNCT ct-10670 107 24 containing contain VERB ct-10670 107 25 10 10 NUM ct-10670 107 26 % % NOUN ct-10670 107 27 fetal fetal ADJ ct-10670 107 28 bovine bovine NOUN ct-10670 107 29 serum serum NOUN ct-10670 107 30 ( ( PUNCT ct-10670 107 31 fbs fbs PROPN ct-10670 107 32 ) ) PUNCT ct-10670 107 33 and and CCONJ ct-10670 107 34 incubated incubate VERB ct-10670 107 35 for for ADP ct-10670 107 36 30 30 NUM ct-10670 107 37 minutes minute NOUN ct-10670 107 38 at at ADP ct-10670 107 39 37oc 37oc NUM ct-10670 107 40 . . PUNCT ct-10670 108 1 the the DET ct-10670 108 2 sperm sperm NOUN ct-10670 108 3 are be AUX ct-10670 108 4 aspirated aspirate VERB ct-10670 108 5 from from ADP ct-10670 108 6 the the DET ct-10670 108 7 upper upper ADJ ct-10670 108 8 swim swim NOUN ct-10670 108 9 - - PUNCT ct-10670 108 10 up up ADP ct-10670 108 11 layer layer NOUN ct-10670 108 12 after after ADP ct-10670 108 13 incubation incubation NOUN ct-10670 108 14 , , PUNCT ct-10670 108 15 and and CCONJ ct-10670 108 16 their their PRON ct-10670 108 17 quality quality NOUN ct-10670 108 18 is be AUX ct-10670 108 19 evaluated evaluate VERB ct-10670 108 20 by by ADP ct-10670 108 21 the the DET ct-10670 108 22 embryologist embryologist NOUN ct-10670 108 23 . . PUNCT ct-10670 109 1 if if SCONJ ct-10670 109 2 needed need VERB ct-10670 109 3 due due ADP ct-10670 109 4 to to ADP ct-10670 109 5 poor poor ADJ ct-10670 109 6 quality quality NOUN ct-10670 109 7 , , PUNCT ct-10670 109 8 another another DET ct-10670 109 9 sample sample NOUN ct-10670 109 10 can can AUX ct-10670 109 11 be be AUX ct-10670 109 12 readily readily ADV ct-10670 109 13 processed process VERB ct-10670 109 14 . . PUNCT ct-10670 110 1 selected select VERB ct-10670 110 2 sperm sperm NOUN ct-10670 110 3 are be AUX ct-10670 110 4 suspended suspend VERB ct-10670 110 5 in in ADP ct-10670 110 6 the the DET ct-10670 110 7 icsi icsi ADJ ct-10670 110 8 dish dish NOUN ct-10670 110 9 in in ADP ct-10670 110 10 a a DET ct-10670 110 11 droplet droplet NOUN ct-10670 110 12 of of ADP ct-10670 110 13 polyvinylpyrrolidone polyvinylpyrrolidone PROPN ct-10670 110 14 ( ( PUNCT ct-10670 110 15 pvp pvp PROPN ct-10670 110 16 , , PUNCT ct-10670 110 17 7 7 NUM ct-10670 110 18 % % NOUN ct-10670 110 19 ) ) PUNCT ct-10670 110 20 , , PUNCT ct-10670 110 21 an an DET ct-10670 110 22 inert inert NOUN ct-10670 110 23 macromolecule macromolecule NOUN ct-10670 110 24 that that PRON ct-10670 110 25 helps help VERB ct-10670 110 26 prevent prevent VERB ct-10670 110 27 sperm sperm NOUN ct-10670 110 28 agglutination agglutination NOUN ct-10670 110 29 and and CCONJ ct-10670 110 30 facilitates facilitate VERB ct-10670 110 31 sperm sperm NOUN ct-10670 110 32 aspiration aspiration NOUN ct-10670 110 33 into into ADP ct-10670 110 34 the the DET ct-10670 110 35 injection injection NOUN ct-10670 110 36 needle needle NOUN ct-10670 110 37 . . PUNCT ct-10670 111 1 it it PRON ct-10670 111 2 is be AUX ct-10670 111 3 important important ADJ ct-10670 111 4 to to PART ct-10670 111 5 note note VERB ct-10670 111 6 that that SCONJ ct-10670 111 7 the the DET ct-10670 111 8 semen semen NOUN ct-10670 111 9 is be AUX ct-10670 111 10 thawed thaw VERB ct-10670 111 11 and and CCONJ ct-10670 111 12 processed process VERB ct-10670 111 13 after after SCONJ ct-10670 111 14 oocytes oocyte NOUN ct-10670 111 15 are be AUX ct-10670 111 16 denuded denude VERB ct-10670 111 17 and and CCONJ ct-10670 111 18 maturation maturation NOUN ct-10670 111 19 is be AUX ct-10670 111 20 assessed assess VERB ct-10670 111 21 . . PUNCT ct-10670 112 1 intracytoplasmic intracytoplasmic ADJ ct-10670 112 2 sperm sperm NOUN ct-10670 112 3 injection injection NOUN ct-10670 112 4 procedures procedure NOUN ct-10670 112 5 oocyte oocyte ADJ ct-10670 112 6 in in ADP ct-10670 112 7 vitro vitro X ct-10670 112 8 maturation maturation NOUN ct-10670 112 9 immature immature ADJ ct-10670 112 10 oocytes oocyte NOUN ct-10670 112 11 are be AUX ct-10670 112 12 placed place VERB ct-10670 112 13 in in ADP ct-10670 112 14 droplets droplet NOUN ct-10670 112 15 of of ADP ct-10670 112 16 maturation maturation NOUN ct-10670 112 17 medium medium NOUN ct-10670 112 18 consisting consist VERB ct-10670 112 19 of of ADP ct-10670 112 20 54.0 54.0 NUM ct-10670 112 21 % % NOUN ct-10670 112 22 dulbecco dulbecco NOUN ct-10670 112 23 ’s ’s PART ct-10670 112 24 modified modified PROPN ct-10670 112 25 eagle eagle PROPN ct-10670 112 26 ’s ’s NOUN ct-10670 112 27 a a DET ct-10670 112 28 b b NOUN ct-10670 112 29 c c X ct-10670 112 30 figure figure NOUN ct-10670 113 1 2 2 NUM ct-10670 113 2 . . X ct-10670 113 3 isothermic isothermic ADJ ct-10670 113 4 shipping shipping NOUN ct-10670 113 5 containers container NOUN ct-10670 113 6 for for ADP ct-10670 113 7 equine equine NOUN ct-10670 113 8 oocyte oocyte ADJ ct-10670 113 9 transport transport NOUN ct-10670 113 10 to to ADP ct-10670 113 11 the the DET ct-10670 113 12 icsi icsi ADJ ct-10670 113 13 laborary laborary NOUN ct-10670 113 14 . . PUNCT ct-10670 114 1 a. a. PROPN ct-10670 114 2 hamilton hamilton PROPN ct-10670 114 3 research research PROPN ct-10670 114 4 equitainer equitainer NOUN ct-10670 114 5 ( ( PUNCT ct-10670 114 6 blue blue ADJ ct-10670 114 7 ) ) PUNCT ct-10670 114 8 and and CCONJ ct-10670 114 9 equocyte equocyte NOUN ct-10670 114 10 ( ( PUNCT ct-10670 114 11 red red ADJ ct-10670 114 12 ) ) PUNCT ct-10670 114 13 transporters transporter NOUN ct-10670 114 14 . . PUNCT ct-10670 115 1 b b NOUN ct-10670 115 2 and and CCONJ ct-10670 115 3 c. c. PROPN ct-10670 115 4 microq microq PROPN ct-10670 115 5 battery battery NOUN ct-10670 115 6 - - PUNCT ct-10670 115 7 powered power VERB ct-10670 115 8 warmed warm VERB ct-10670 115 9 transporter transporter NOUN ct-10670 115 10 system system NOUN ct-10670 115 11 . . PUNCT ct-10670 116 1 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PROPN ct-10670 116 2 citation citation NOUN ct-10670 116 3 : : PUNCT ct-10670 116 4 clinical clinical ADJ ct-10670 116 5 theriogenology theriogenology NOUN ct-10670 116 6 2024 2024 NUM ct-10670 116 7 , , PUNCT ct-10670 116 8 16 16 NUM ct-10670 116 9 , , PUNCT ct-10670 116 10 10670 10670 NUM ct-10670 116 11 , , PUNCT ct-10670 116 12 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 X ct-10670 116 13 5 5 NUM ct-10670 116 14 medium medium NOUN ct-10670 116 15 ( ( PUNCT ct-10670 116 16 dmem)/f-12 dmem)/f-12 NOUN ct-10670 116 17 with with ADP ct-10670 116 18 15 15 NUM ct-10670 116 19 mm mm PROPN ct-10670 116 20 hepes hepe NOUN ct-10670 116 21 , , PUNCT ct-10670 116 22 25 25 NUM ct-10670 116 23 mg mg NOUN ct-10670 116 24 ml-1 ml-1 ADV ct-10670 116 25 gentamicin gentamicin PROPN ct-10670 116 26 , , PUNCT ct-10670 116 27 36.0 36.0 NUM ct-10670 116 28 % % NOUN ct-10670 116 29 global global ADJ ct-10670 116 30 medium medium ADJ ct-10670 116 31 ( ( PUNCT ct-10670 116 32 lifeglobal lifeglobal ADJ ct-10670 116 33 ) ) PUNCT ct-10670 116 34 , , PUNCT ct-10670 116 35 0.1 0.1 NUM ct-10670 116 36 mm mm NOUN ct-10670 116 37 sodium sodium NOUN ct-10670 116 38 pyruvate pyruvate NOUN ct-10670 116 39 , , PUNCT ct-10670 116 40 6.0 6.0 NUM ct-10670 116 41 % % NOUN ct-10670 116 42 fetal fetal ADJ ct-10670 116 43 bovine bovine NOUN ct-10670 116 44 serum serum NOUN ct-10670 116 45 ( ( PUNCT ct-10670 116 46 fbs fb VERB ct-10670 116 47 ; ; PUNCT ct-10670 116 48 f2442 f2442 NUM ct-10670 116 49 ; ; PUNCT ct-10670 116 50 sigma sigma PROPN ct-10670 116 51 ) ) PUNCT ct-10670 116 52 , , PUNCT ct-10670 116 53 10 10 NUM ct-10670 116 54 ml ml NOUN ct-10670 116 55 ml-1 ml-1 PRON ct-10670 116 56 insulin insulin NOUN ct-10670 116 57 – – PUNCT ct-10670 116 58 transferrin transferrin NOUN ct-10670 116 59 – – PUNCT ct-10670 116 60 selenium selenium NOUN ct-10670 116 61 ( ( PUNCT ct-10670 116 62 its its PRON ct-10670 116 63 ) ) PUNCT ct-10670 116 64 solution solution NOUN ct-10670 116 65 , , PUNCT ct-10670 116 66 10 10 NUM ct-10670 116 67 % % NOUN ct-10670 116 68 dominant dominant ADJ ct-10670 116 69 stimulated stimulate VERB ct-10670 116 70 follicle follicle NOUN ct-10670 116 71 follicular follicular ADJ ct-10670 116 72 fluid fluid NOUN ct-10670 116 73 , , PUNCT ct-10670 116 74 8.8 8.8 NUM ct-10670 116 75 mu mu NOUN ct-10670 116 76 ovine ovine PROPN ct-10670 116 77 fsh fsh PROPN ct-10670 116 78 ( ( PUNCT ct-10670 116 79 national national ADJ ct-10670 116 80 hormone hormone NOUN ct-10670 116 81 and and CCONJ ct-10670 116 82 peptide peptide NOUN ct-10670 116 83 program program NOUN ct-10670 116 84 ) ) PUNCT ct-10670 116 85 and and CCONJ ct-10670 116 86 1.1 1.1 NUM ct-10670 116 87 mu mu NOUN ct-10670 116 88 ml-1 ml-1 ADV ct-10670 116 89 porcine porcine NOUN ct-10670 116 90 somatotropin somatotropin NOUN ct-10670 116 91 ( ( PUNCT ct-10670 116 92 harborucla harborucla NOUN ct-10670 116 93 research research NOUN ct-10670 116 94 and and CCONJ ct-10670 116 95 education education PROPN ct-10670 116 96 institute institute PROPN ct-10670 116 97 ) ) PUNCT ct-10670 116 98 , , PUNCT ct-10670 116 99 as as SCONJ ct-10670 116 100 described.11,13 described.11,13 NOUN ct-10670 116 101 the the DET ct-10670 116 102 morning morning NOUN ct-10670 116 103 after after ADP ct-10670 116 104 aspiration aspiration NOUN ct-10670 116 105 and and CCONJ ct-10670 116 106 overnight overnight ADJ ct-10670 116 107 holding holding NOUN ct-10670 116 108 , , PUNCT ct-10670 116 109 protected protect VERB ct-10670 116 110 from from ADP ct-10670 116 111 light light ADJ ct-10670 116 112 , , PUNCT ct-10670 116 113 oocytes oocyte NOUN ct-10670 116 114 are be AUX ct-10670 116 115 moved move VERB ct-10670 116 116 from from ADP ct-10670 116 117 holding hold VERB ct-10670 116 118 medium medium NOUN ct-10670 116 119 in in ADP ct-10670 116 120 22oc 22oc ADJ ct-10670 116 121 to to ADP ct-10670 116 122 38.2oc 38.2oc NUM ct-10670 116 123 in in ADP ct-10670 116 124 a a DET ct-10670 116 125 triple triple ADJ ct-10670 116 126 gas gas NOUN ct-10670 116 127 incubator incubator NOUN ct-10670 116 128 using use VERB ct-10670 116 129 n2 n2 ADJ ct-10670 116 130 gas gas NOUN ct-10670 116 131 to to PART ct-10670 116 132 lower lower VERB ct-10670 116 133 oxygen oxygen NOUN ct-10670 116 134 tension tension NOUN ct-10670 116 135 ( ( PUNCT ct-10670 116 136 5.8 5.8 NUM ct-10670 116 137 % % NOUN ct-10670 116 138 co2 co2 NOUN ct-10670 116 139 , , PUNCT ct-10670 116 140 5%o2 5%o2 NUM ct-10670 116 141 , , PUNCT ct-10670 116 142 and and CCONJ ct-10670 116 143 89.2 89.2 NUM ct-10670 116 144 % % NOUN ct-10670 116 145 n2 n2 NOUN ct-10670 116 146 ) ) PUNCT ct-10670 116 147 for for ADP ct-10670 116 148 maturation maturation NOUN ct-10670 116 149 . . PUNCT ct-10670 117 1 oocytes oocyte NOUN ct-10670 117 2 remain remain VERB ct-10670 117 3 in in ADP ct-10670 117 4 maturation maturation NOUN ct-10670 117 5 medium medium NOUN ct-10670 117 6 for for ADP ct-10670 117 7 27 27 NUM ct-10670 117 8 - - SYM ct-10670 117 9 30 30 NUM ct-10670 117 10 hours hour NOUN ct-10670 117 11 , , PUNCT ct-10670 117 12 then then ADV ct-10670 117 13 cumulus cumulus NOUN ct-10670 117 14 oocyte oocyte ADJ ct-10670 117 15 complexes complex NOUN ct-10670 117 16 are be AUX ct-10670 117 17 denuded denude VERB ct-10670 117 18 using use VERB ct-10670 117 19 hyaluronidase hyaluronidase NOUN ct-10670 117 20 ( ( PUNCT ct-10670 117 21 hyaluronidase hyaluronidase INTJ ct-10670 117 22 0.2%/ml 0.2%/ml PUNCT ct-10670 118 1 [ [ X ct-10670 118 2 sigma sigma PROPN ct-10670 118 3 aldrich aldrich PROPN ct-10670 118 4 h3506 h3506 PROPN ct-10670 118 5 - - PUNCT ct-10670 118 6 1 1 NUM ct-10670 118 7 g g NOUN ct-10670 118 8 ] ] X ct-10670 118 9 ; ; PUNCT ct-10670 118 10 2 2 NUM ct-10670 118 11 - - PUNCT ct-10670 118 12 minute minute NOUN ct-10670 118 13 incubation incubation NOUN ct-10670 118 14 ) ) PUNCT ct-10670 118 15 and and CCONJ ct-10670 118 16 mechanical mechanical ADJ ct-10670 118 17 pipetting pipetting NOUN ct-10670 118 18 in in ADP ct-10670 118 19 a a DET ct-10670 118 20 4 4 NUM ct-10670 118 21 - - PUNCT ct-10670 118 22 well well NOUN ct-10670 118 23 dish dish NOUN ct-10670 118 24 . . PUNCT ct-10670 119 1 the the DET ct-10670 119 2 first first ADJ ct-10670 119 3 polar polar ADJ ct-10670 119 4 body body NOUN ct-10670 119 5 , , PUNCT ct-10670 119 6 if if SCONJ ct-10670 119 7 present present ADJ ct-10670 119 8 , , PUNCT ct-10670 119 9 can can AUX ct-10670 119 10 then then ADV ct-10670 119 11 be be AUX ct-10670 119 12 visualized visualize VERB ct-10670 119 13 between between ADP ct-10670 119 14 the the DET ct-10670 119 15 oolemma oolemma PROPN ct-10670 119 16 and and CCONJ ct-10670 119 17 zona zona PROPN ct-10670 119 18 pellucida pellucida PROPN ct-10670 119 19 , , PUNCT ct-10670 119 20 known know VERB ct-10670 119 21 as as ADP ct-10670 119 22 the the DET ct-10670 119 23 perivitelline perivitelline ADJ ct-10670 119 24 space space NOUN ct-10670 119 25 , , PUNCT ct-10670 119 26 using use VERB ct-10670 119 27 a a DET ct-10670 119 28 magnification magnification NOUN ct-10670 119 29 of of ADP ct-10670 119 30 20 20 NUM ct-10670 119 31 x x PUNCT ct-10670 119 32 with with ADP ct-10670 119 33 brightfield brightfield NOUN ct-10670 119 34 optics optic NOUN ct-10670 119 35 on on ADP ct-10670 119 36 an an DET ct-10670 119 37 olympus olympus PROPN ct-10670 119 38 ix-70 ix-70 NUM ct-10670 119 39 microscope microscope NOUN ct-10670 119 40 . . PUNCT ct-10670 120 1 denuding denude VERB ct-10670 120 2 the the DET ct-10670 120 3 surrounding surround VERB ct-10670 120 4 cumulus cumulus NOUN ct-10670 120 5 cells cell NOUN ct-10670 120 6 is be AUX ct-10670 120 7 necessary necessary ADJ ct-10670 120 8 to to PART ct-10670 120 9 visualize visualize VERB ct-10670 120 10 the the DET ct-10670 120 11 oolemma oolemma NOUN ct-10670 120 12 and and CCONJ ct-10670 120 13 perform perform VERB ct-10670 120 14 icsi icsi NOUN ct-10670 120 15 . . PUNCT ct-10670 121 1 this this PRON ct-10670 121 2 is be AUX ct-10670 121 3 in in ADP ct-10670 121 4 contrast contrast NOUN ct-10670 121 5 to to ADP ct-10670 121 6 traditional traditional ADJ ct-10670 121 7 ivf ivf NOUN ct-10670 121 8 , , PUNCT ct-10670 121 9 in in ADP ct-10670 121 10 which which PRON ct-10670 121 11 the the DET ct-10670 121 12 cumulus cumulus NOUN ct-10670 121 13 supports support VERB ct-10670 121 14 , , PUNCT ct-10670 121 15 and and CCONJ ct-10670 121 16 is be AUX ct-10670 121 17 necessary necessary ADJ ct-10670 121 18 for for ADP ct-10670 121 19 fertilization fertilization NOUN ct-10670 121 20 , , PUNCT ct-10670 121 21 as as SCONJ ct-10670 121 22 it it PRON ct-10670 121 23 provides provide VERB ct-10670 121 24 natural natural ADJ ct-10670 121 25 sperm sperm NOUN ct-10670 121 26 selection selection NOUN ct-10670 121 27 and and CCONJ ct-10670 121 28 prevents prevent VERB ct-10670 121 29 polyspermy polyspermy ADJ ct-10670 121 30 . . PUNCT ct-10670 122 1 after after ADP ct-10670 122 2 the the DET ct-10670 122 3 maturation maturation NOUN ct-10670 122 4 period period NOUN ct-10670 122 5 of of ADP ct-10670 122 6 a a DET ct-10670 122 7 batch batch NOUN ct-10670 122 8 of of ADP ct-10670 122 9 oocytes oocyte NOUN ct-10670 122 10 , , PUNCT ct-10670 122 11 they they PRON ct-10670 122 12 can can AUX ct-10670 122 13 not not PART ct-10670 122 14 be be AUX ct-10670 122 15 assumed assume VERB ct-10670 122 16 to to PART ct-10670 122 17 be be AUX ct-10670 122 18 in in ADP ct-10670 122 19 the the DET ct-10670 122 20 same same ADJ ct-10670 122 21 stage stage NOUN ct-10670 122 22 of of ADP ct-10670 122 23 meiosis meiosis NOUN ct-10670 122 24 . . PUNCT ct-10670 123 1 occasionally occasionally ADV ct-10670 123 2 , , PUNCT ct-10670 123 3 we we PRON ct-10670 123 4 observe observe VERB ct-10670 123 5 that that SCONJ ct-10670 123 6 even even ADV ct-10670 123 7 10 10 NUM ct-10670 123 8 minutes minute NOUN ct-10670 123 9 of of ADP ct-10670 123 10 additional additional ADJ ct-10670 123 11 ivm ivm PROPN ct-10670 123 12 culture culture NOUN ct-10670 123 13 may may AUX ct-10670 123 14 be be AUX ct-10670 123 15 sufficient sufficient ADJ ct-10670 123 16 for for ADP ct-10670 123 17 the the DET ct-10670 123 18 first first ADJ ct-10670 123 19 polar polar ADJ ct-10670 123 20 body body NOUN ct-10670 123 21 extrusion extrusion NOUN ct-10670 123 22 to to PART ct-10670 123 23 occur occur VERB ct-10670 123 24 if if SCONJ ct-10670 123 25 not not PART ct-10670 123 26 observed observe VERB ct-10670 123 27 during during ADP ct-10670 123 28 the the DET ct-10670 123 29 initial initial ADJ ct-10670 123 30 assessment assessment NOUN ct-10670 123 31 . . PUNCT ct-10670 124 1 on on ADP ct-10670 124 2 the the DET ct-10670 124 3 other other ADJ ct-10670 124 4 hand hand NOUN ct-10670 124 5 , , PUNCT ct-10670 124 6 some some DET ct-10670 124 7 oocytes oocyte NOUN ct-10670 124 8 might might AUX ct-10670 124 9 already already ADV ct-10670 124 10 be be AUX ct-10670 124 11 atretic atretic ADJ ct-10670 124 12 , , PUNCT ct-10670 124 13 and and CCONJ ct-10670 124 14 thus thus ADV ct-10670 124 15 fail fail VERB ct-10670 124 16 to to PART ct-10670 124 17 extrude extrude VERB ct-10670 124 18 a a DET ct-10670 124 19 polar polar ADJ ct-10670 124 20 body body NOUN ct-10670 124 21 regardless regardless ADV ct-10670 124 22 of of ADP ct-10670 124 23 any any DET ct-10670 124 24 additional additional ADJ ct-10670 124 25 time time NOUN ct-10670 124 26 . . PUNCT ct-10670 125 1 the the DET ct-10670 125 2 appearance appearance NOUN ct-10670 125 3 of of ADP ct-10670 125 4 the the DET ct-10670 125 5 first first ADJ ct-10670 125 6 polar polar ADJ ct-10670 125 7 body body NOUN ct-10670 125 8 defines define VERB ct-10670 125 9 the the DET ct-10670 125 10 meiotic meiotic ADJ ct-10670 125 11 stage stage NOUN ct-10670 125 12 of of ADP ct-10670 125 13 the the DET ct-10670 125 14 oocyte oocyte NOUN ct-10670 125 15 and and CCONJ ct-10670 125 16 consequently consequently ADV ct-10670 125 17 its its PRON ct-10670 125 18 readiness readiness NOUN ct-10670 125 19 to to PART ct-10670 125 20 be be AUX ct-10670 125 21 fertilized fertilize VERB ct-10670 125 22 by by ADP ct-10670 125 23 injection injection NOUN ct-10670 125 24 of of ADP ct-10670 125 25 a a DET ct-10670 125 26 single single ADJ ct-10670 125 27 sperm sperm NOUN ct-10670 125 28 . . PUNCT ct-10670 126 1 all all DET ct-10670 126 2 oocytes oocyte NOUN ct-10670 126 3 presenting present VERB ct-10670 126 4 this this DET ct-10670 126 5 morphology morphology NOUN ct-10670 126 6 are be AUX ct-10670 126 7 injected inject VERB ct-10670 126 8 with with ADP ct-10670 126 9 a a DET ct-10670 126 10 single single ADJ ct-10670 126 11 sperm sperm NOUN ct-10670 126 12 ( ( PUNCT ct-10670 126 13 figure figure NOUN ct-10670 126 14 3 3 NUM ct-10670 126 15 ) ) PUNCT ct-10670 126 16 . . PUNCT ct-10670 127 1 our our PRON ct-10670 127 2 laboratory laboratory NOUN ct-10670 127 3 uses use VERB ct-10670 127 4 a a DET ct-10670 127 5 time time NOUN ct-10670 127 6 - - PUNCT ct-10670 127 7 lapse lapse NOUN ct-10670 127 8 imaging imaging NOUN ct-10670 127 9 system system NOUN ct-10670 127 10 to to PART ct-10670 127 11 observe observe VERB ct-10670 127 12 oocyte oocyte ADJ ct-10670 127 13 maturation maturation NOUN ct-10670 127 14 inside inside ADP ct-10670 127 15 the the DET ct-10670 127 16 incubator incubator NOUN ct-10670 127 17 which which PRON ct-10670 127 18 has have AUX ct-10670 127 19 allowed allow VERB ct-10670 127 20 us we PRON ct-10670 127 21 to to PART ct-10670 127 22 observe observe VERB ct-10670 127 23 cumulus cumulus NOUN ct-10670 127 24 expansion expansion NOUN ct-10670 127 25 ( ( PUNCT ct-10670 127 26 figure figure NOUN ct-10670 127 27 4 4 NUM ct-10670 127 28 ) ) PUNCT ct-10670 127 29 and and CCONJ ct-10670 127 30 polar polar ADJ ct-10670 127 31 body body NOUN ct-10670 127 32 extrusion extrusion NOUN ct-10670 127 33 in in ADP ct-10670 127 34 near near ADJ ct-10670 127 35 - - PUNCT ct-10670 127 36 real real ADJ ct-10670 127 37 time time NOUN ct-10670 127 38 . . PUNCT ct-10670 128 1 we we PRON ct-10670 128 2 have have AUX ct-10670 128 3 measured measure VERB ct-10670 128 4 rates rate NOUN ct-10670 128 5 of of ADP ct-10670 128 6 cumulus cumulus ADJ ct-10670 128 7 - - PUNCT ct-10670 128 8 oocyte oocyte ADJ ct-10670 128 9 complex complex NOUN ct-10670 128 10 ( ( PUNCT ct-10670 128 11 coc coc ADJ ct-10670 128 12 ) ) PUNCT ct-10670 128 13 expansion expansion NOUN ct-10670 128 14 . . PUNCT ct-10670 129 1 similarly similarly ADV ct-10670 129 2 , , PUNCT ct-10670 129 3 we we PRON ct-10670 129 4 elucidated elucidate VERB ct-10670 129 5 the the DET ct-10670 129 6 dynamics dynamic NOUN ct-10670 129 7 of of ADP ct-10670 129 8 genetic genetic ADJ ct-10670 129 9 expression expression NOUN ct-10670 129 10 in in ADP ct-10670 129 11 the the DET ct-10670 129 12 cumulus cumulus NOUN ct-10670 129 13 cells cell NOUN ct-10670 129 14 and and CCONJ ct-10670 129 15 oocytes oocyte NOUN ct-10670 129 16 during during ADP ct-10670 129 17 maturation maturation NOUN ct-10670 129 18 in in ADP ct-10670 129 19 vitro vitro X ct-10670 129 20 and and CCONJ ct-10670 129 21 in in ADP ct-10670 129 22 vivo vivo NOUN ct-10670 129 23 after after ADP ct-10670 129 24 induction induction NOUN ct-10670 129 25 with with ADP ct-10670 129 26 gnrh gnrh NOUN ct-10670 129 27 analogs analog NOUN ct-10670 129 28 . . PUNCT ct-10670 130 1 this this PRON ct-10670 130 2 unraveled unravel VERB ct-10670 130 3 important important ADJ ct-10670 130 4 biological biological ADJ ct-10670 130 5 pathways pathway NOUN ct-10670 130 6 critical critical ADJ ct-10670 130 7 for for ADP ct-10670 130 8 the the DET ct-10670 130 9 proper proper ADJ ct-10670 130 10 development development NOUN ct-10670 130 11 of of ADP ct-10670 130 12 the the DET ct-10670 130 13 oocyte oocyte NOUN ct-10670 130 14 and and CCONJ ct-10670 130 15 provided provide VERB ct-10670 130 16 some some DET ct-10670 130 17 clues clue NOUN ct-10670 130 18 about about ADP ct-10670 130 19 the the DET ct-10670 130 20 main main ADJ ct-10670 130 21 difference difference NOUN ct-10670 130 22 between between ADP ct-10670 130 23 the the DET ct-10670 130 24 in in ADP ct-10670 130 25 vitro vitro X ct-10670 130 26 and and CCONJ ct-10670 130 27 in in ADP ct-10670 130 28 vivo vivo NOUN ct-10670 130 29 environments.27 environments.27 PROPN ct-10670 130 30 intracytoplasmic intracytoplasmic ADJ ct-10670 130 31 sperm sperm NOUN ct-10670 130 32 injection injection NOUN ct-10670 130 33 for for ADP ct-10670 130 34 every every DET ct-10670 130 35 icsi icsi ADJ ct-10670 130 36 procedure procedure NOUN ct-10670 130 37 , , PUNCT ct-10670 130 38 we we PRON ct-10670 130 39 prepare prepare VERB ct-10670 130 40 a a DET ct-10670 130 41 dish dish NOUN ct-10670 130 42 with with ADP ct-10670 130 43 microdroplets microdroplet NOUN ct-10670 130 44 of of ADP ct-10670 130 45 g g NOUN ct-10670 130 46 - - PUNCT ct-10670 130 47 mops-10 mops-10 PROPN ct-10670 130 48 % % NOUN ct-10670 130 49 fbs fbs NOUN ct-10670 130 50 for for ADP ct-10670 130 51 injection injection NOUN ct-10670 130 52 and and CCONJ ct-10670 130 53 pvp pvp VERB ct-10670 130 54 7 7 NUM ct-10670 130 55 % % NOUN ct-10670 130 56 for for ADP ct-10670 130 57 semen semen NOUN ct-10670 130 58 pickup pickup NOUN ct-10670 130 59 ; ; PUNCT ct-10670 130 60 both both PRON ct-10670 130 61 of of ADP ct-10670 130 62 these these DET ct-10670 130 63 media medium NOUN ct-10670 130 64 are be AUX ct-10670 130 65 buffered buffer VERB ct-10670 130 66 to to PART ct-10670 130 67 maintain maintain VERB ct-10670 130 68 the the DET ct-10670 130 69 ph ph NOUN ct-10670 130 70 while while SCONJ ct-10670 130 71 they they PRON ct-10670 130 72 are be AUX ct-10670 130 73 outside outside ADP ct-10670 130 74 the the DET ct-10670 130 75 incubator incubator NOUN ct-10670 130 76 environment environment NOUN ct-10670 130 77 . . PUNCT ct-10670 131 1 although although SCONJ ct-10670 131 2 the the DET ct-10670 131 3 micromanipulator micromanipulator NOUN ct-10670 131 4 - - PUNCT ct-10670 131 5 microscope microscope NOUN ct-10670 131 6 system system NOUN ct-10670 131 7 ( ( PUNCT ct-10670 131 8 figure figure NOUN ct-10670 131 9 5 5 NUM ct-10670 131 10 ) ) PUNCT ct-10670 131 11 is be AUX ct-10670 131 12 equipped equip VERB ct-10670 131 13 with with ADP ct-10670 131 14 a a DET ct-10670 131 15 heating heating NOUN ct-10670 131 16 stage stage NOUN ct-10670 131 17 , , PUNCT ct-10670 131 18 each each DET ct-10670 131 19 minute minute NOUN ct-10670 131 20 spent spend VERB ct-10670 131 21 outside outside ADP ct-10670 131 22 the the DET ct-10670 131 23 incubator incubator NOUN ct-10670 131 24 decreases decrease VERB ct-10670 131 25 the the DET ct-10670 131 26 chances chance NOUN ct-10670 131 27 of of ADP ct-10670 131 28 obtaining obtain VERB ct-10670 131 29 blastocysts blastocyst NOUN ct-10670 131 30 , , PUNCT ct-10670 131 31 thus thus ADV ct-10670 131 32 this this DET ct-10670 131 33 step step NOUN ct-10670 131 34 needs need VERB ct-10670 131 35 to to PART ct-10670 131 36 be be AUX ct-10670 131 37 performed perform VERB ct-10670 131 38 efficiently efficiently ADV ct-10670 131 39 and and CCONJ ct-10670 131 40 quickly quickly ADV ct-10670 131 41 . . PUNCT ct-10670 132 1 when when SCONJ ct-10670 132 2 the the DET ct-10670 132 3 swim swim NOUN ct-10670 132 4 - - PUNCT ct-10670 132 5 up up ADP ct-10670 132 6 process process NOUN ct-10670 132 7 ends end VERB ct-10670 132 8 and and CCONJ ct-10670 132 9 a a DET ct-10670 132 10 good good ADJ ct-10670 132 11 quality quality NOUN ct-10670 132 12 sample sample NOUN ct-10670 132 13 of of ADP ct-10670 132 14 sperm sperm NOUN ct-10670 132 15 is be AUX ct-10670 132 16 obtained obtain VERB ct-10670 132 17 , , PUNCT ct-10670 132 18 mature mature ADJ ct-10670 132 19 oocytes oocyte NOUN ct-10670 132 20 are be AUX ct-10670 132 21 individually individually ADV ct-10670 132 22 placed place VERB ct-10670 132 23 in in ADP ct-10670 132 24 microdroplets microdroplet NOUN ct-10670 132 25 ( ( PUNCT ct-10670 132 26 4 4 NUM ct-10670 132 27 μl μl PRON ct-10670 132 28 ) ) PUNCT ct-10670 132 29 of of ADP ct-10670 132 30 g g NOUN ct-10670 132 31 - - PUNCT ct-10670 132 32 mops mop NOUN ct-10670 132 33 10 10 NUM ct-10670 132 34 % % NOUN ct-10670 132 35 fbs fbs NOUN ct-10670 132 36 that that PRON ct-10670 132 37 are be AUX ct-10670 132 38 laid lay VERB ct-10670 132 39 out out ADP ct-10670 132 40 in in ADP ct-10670 132 41 the the DET ct-10670 132 42 icsi icsi ADJ ct-10670 132 43 dish dish NOUN ct-10670 132 44 , , PUNCT ct-10670 132 45 while while SCONJ ct-10670 132 46 3 3 NUM ct-10670 132 47 μl μl NOUN ct-10670 132 48 of of ADP ct-10670 132 49 semen semen NOUN ct-10670 132 50 is be AUX ct-10670 132 51 placed place VERB ct-10670 132 52 in in ADP ct-10670 132 53 the the DET ct-10670 132 54 droplet droplet NOUN ct-10670 132 55 of of ADP ct-10670 132 56 pvp pvp PROPN ct-10670 132 57 ( ( PUNCT ct-10670 132 58 sperm sperm NOUN ct-10670 132 59 droplet droplet ADJ ct-10670 132 60 ) ) PUNCT ct-10670 132 61 . . PUNCT ct-10670 133 1 the the DET ct-10670 133 2 first first ADJ ct-10670 133 3 step step NOUN ct-10670 133 4 is be AUX ct-10670 133 5 the the DET ct-10670 133 6 immobilization immobilization NOUN ct-10670 133 7 of of ADP ct-10670 133 8 the the DET ct-10670 133 9 targeted target VERB ct-10670 133 10 sperm sperm NOUN ct-10670 133 11 by by ADP ct-10670 133 12 pressing press VERB ct-10670 133 13 or or CCONJ ct-10670 133 14 trapping trap VERB ct-10670 133 15 the the DET ct-10670 133 16 midpiece/ midpiece/ NUM ct-10670 133 17 tail tail NOUN ct-10670 133 18 with with ADP ct-10670 133 19 the the DET ct-10670 133 20 injection injection NOUN ct-10670 133 21 needle needle NOUN ct-10670 133 22 against against ADP ct-10670 133 23 the the DET ct-10670 133 24 bottom bottom NOUN ct-10670 133 25 of of ADP ct-10670 133 26 the the DET ct-10670 133 27 dish dish NOUN ct-10670 133 28 . . PUNCT ct-10670 134 1 this this PRON ct-10670 134 2 immobilizes immobilize VERB ct-10670 134 3 the the DET ct-10670 134 4 sperm sperm NOUN ct-10670 134 5 and and CCONJ ct-10670 134 6 creates create VERB ct-10670 134 7 a a DET ct-10670 134 8 visible visible ADJ ct-10670 134 9 bend bend NOUN ct-10670 134 10 in in ADP ct-10670 134 11 the the DET ct-10670 134 12 tail tail NOUN ct-10670 134 13 . . PUNCT ct-10670 135 1 then then ADV ct-10670 135 2 , , PUNCT ct-10670 135 3 the the DET ct-10670 135 4 sperm sperm NOUN ct-10670 135 5 is be AUX ct-10670 135 6 picked pick VERB ct-10670 135 7 up up ADP ct-10670 135 8 with with ADP ct-10670 135 9 the the DET ct-10670 135 10 injection injection NOUN ct-10670 135 11 needle needle NOUN ct-10670 135 12 by by ADP ct-10670 135 13 aspirating aspirate VERB ct-10670 135 14 the the DET ct-10670 135 15 tail tail NOUN ct-10670 135 16 first first ADV ct-10670 135 17 . . PUNCT ct-10670 136 1 next next ADV ct-10670 136 2 , , PUNCT ct-10670 136 3 the the DET ct-10670 136 4 dish dish NOUN ct-10670 136 5 is be AUX ct-10670 136 6 repositioned reposition VERB ct-10670 136 7 to to PART ct-10670 136 8 center center VERB ct-10670 136 9 the the DET ct-10670 136 10 image image NOUN ct-10670 136 11 in in ADP ct-10670 136 12 a a DET ct-10670 136 13 droplet droplet ADJ ct-10670 136 14 containing contain VERB ct-10670 136 15 one one NUM ct-10670 136 16 of of ADP ct-10670 136 17 the the DET ct-10670 136 18 mii mii PROPN ct-10670 136 19 oocytes oocyte NOUN ct-10670 136 20 , , PUNCT ct-10670 136 21 and and CCONJ ct-10670 136 22 with with ADP ct-10670 136 23 the the DET ct-10670 136 24 holding hold VERB ct-10670 136 25 pipette pipette NOUN ct-10670 136 26 , , PUNCT ct-10670 136 27 the the DET ct-10670 136 28 oocyte oocyte NOUN ct-10670 136 29 is be AUX ct-10670 136 30 held hold VERB ct-10670 136 31 from from ADP ct-10670 136 32 one one NUM ct-10670 136 33 side side NOUN ct-10670 136 34 ( ( PUNCT ct-10670 136 35 9 9 NUM ct-10670 136 36 o’clock o’clock NOUN ct-10670 136 37 ) ) PUNCT ct-10670 136 38 . . PUNCT ct-10670 137 1 oocyte oocyte NOUN ct-10670 137 2 is be AUX ct-10670 137 3 rotated rotate VERB ct-10670 137 4 in in ADP ct-10670 137 5 a a DET ct-10670 137 6 manner manner NOUN ct-10670 137 7 that that SCONJ ct-10670 137 8 the the DET ct-10670 137 9 polar polar ADJ ct-10670 137 10 body body NOUN ct-10670 137 11 is be AUX ct-10670 137 12 situated situate VERB ct-10670 137 13 at at ADP ct-10670 137 14 either either CCONJ ct-10670 137 15 6 6 NUM ct-10670 137 16 or or CCONJ ct-10670 137 17 12 12 NUM ct-10670 137 18 o’clock o’clock NOUN ct-10670 137 19 position position NOUN ct-10670 137 20 , , PUNCT ct-10670 137 21 leaving leave VERB ct-10670 137 22 the the DET ct-10670 137 23 other other ADJ ct-10670 137 24 side side NOUN ct-10670 137 25 ( ( PUNCT ct-10670 137 26 3 3 NUM ct-10670 137 27 o’clock o’clock NOUN ct-10670 137 28 ) ) PUNCT ct-10670 137 29 free free ADJ ct-10670 137 30 for for ADP ct-10670 137 31 inserting insert VERB ct-10670 137 32 the the DET ct-10670 137 33 injection injection NOUN ct-10670 137 34 needle needle NOUN ct-10670 137 35 and and CCONJ ct-10670 137 36 depositing deposit VERB ct-10670 137 37 the the DET ct-10670 137 38 sperm sperm NOUN ct-10670 137 39 . . PUNCT ct-10670 138 1 in in ADP ct-10670 138 2 our our PRON ct-10670 138 3 laboratory laboratory NOUN ct-10670 138 4 , , PUNCT ct-10670 138 5 we we PRON ct-10670 138 6 use use VERB ct-10670 138 7 conventional conventional ADJ ct-10670 138 8 icsi icsi NOUN ct-10670 138 9 that that PRON ct-10670 138 10 requires require VERB ct-10670 138 11 an an DET ct-10670 138 12 injection injection NOUN ct-10670 138 13 needle needle NOUN ct-10670 138 14 sharp sharp ADJ ct-10670 138 15 enough enough ADV ct-10670 138 16 to to PART ct-10670 138 17 penetrate penetrate VERB ct-10670 138 18 the the DET ct-10670 138 19 zona zona PROPN ct-10670 138 20 pellucida pellucida PROPN ct-10670 138 21 and and CCONJ ct-10670 138 22 oocyte oocyte ADJ ct-10670 138 23 plasma plasma NOUN ct-10670 138 24 membrane membrane NOUN ct-10670 138 25 ( ( PUNCT ct-10670 138 26 oolemma oolemma PROPN ct-10670 138 27 ) ) PUNCT ct-10670 138 28 . . PUNCT ct-10670 139 1 some some DET ct-10670 139 2 laboratories laboratory NOUN ct-10670 139 3 report report VERB ct-10670 139 4 improved improve VERB ct-10670 139 5 embryo embryo NOUN ct-10670 139 6 cleavage cleavage NOUN ct-10670 139 7 and and CCONJ ct-10670 139 8 subsequent subsequent ADJ ct-10670 139 9 development development NOUN ct-10670 139 10 using use VERB ct-10670 139 11 a a DET ct-10670 139 12 piezo piezo X ct-10670 139 13 drive drive NOUN ct-10670 139 14 that that SCONJ ct-10670 139 15 gently gently ADV ct-10670 139 16 ‘ ' PUNCT ct-10670 139 17 drills drill VERB ct-10670 139 18 ’ ' PUNCT ct-10670 139 19 the the DET ct-10670 139 20 injection injection NOUN ct-10670 139 21 pipette pipette NOUN ct-10670 139 22 into into ADP ct-10670 139 23 and and CCONJ ct-10670 139 24 through through ADP ct-10670 139 25 the the DET ct-10670 139 26 zona zona PROPN ct-10670 139 27 pellucida pellucida PROPN ct-10670 139 28 while while SCONJ ct-10670 139 29 maintaining maintain VERB ct-10670 139 30 the the DET ct-10670 139 31 membrane membrane NOUN ct-10670 139 32 integrity integrity NOUN ct-10670 139 33 of of ADP ct-10670 139 34 the the DET ct-10670 139 35 oolemma oolemma NOUN ct-10670 139 36 and and CCONJ ct-10670 139 37 inducing induce VERB ct-10670 139 38 activation activation NOUN ct-10670 139 39 of of ADP ct-10670 139 40 the the DET ct-10670 139 41 oocyte oocyte NOUN ct-10670 139 42 to to ADP ct-10670 139 43 some some DET ct-10670 139 44 extent.7,11 extent.7,11 PROPN ct-10670 139 45 in in ADP ct-10670 139 46 our our PRON ct-10670 139 47 hands hand NOUN ct-10670 139 48 , , PUNCT ct-10670 139 49 the the DET ct-10670 139 50 piezo piezo PROPN ct-10670 139 51 drive drive NOUN ct-10670 139 52 has have AUX ct-10670 139 53 not not PART ct-10670 139 54 resulted result VERB ct-10670 139 55 in in ADP ct-10670 139 56 improved improved ADJ ct-10670 139 57 blastocyst blastocyst NOUN ct-10670 139 58 rates rate NOUN ct-10670 139 59 for for ADP ct-10670 139 60 equine equine NOUN ct-10670 139 61 zygotes zygote NOUN ct-10670 139 62 and and CCONJ ct-10670 139 63 appears appear VERB ct-10670 139 64 to to PART ct-10670 139 65 be be AUX ct-10670 139 66 highly highly ADV ct-10670 139 67 dependent dependent ADJ ct-10670 139 68 on on ADP ct-10670 139 69 the the DET ct-10670 139 70 skills skill NOUN ct-10670 139 71 of of ADP ct-10670 139 72 the the DET ct-10670 139 73 embryologist embryologist NOUN ct-10670 139 74 performing perform VERB ct-10670 139 75 icsi icsi NOUN ct-10670 139 76 . . PUNCT ct-10670 140 1 after after SCONJ ct-10670 140 2 the the DET ct-10670 140 3 sperm sperm NOUN ct-10670 140 4 is be AUX ct-10670 140 5 injected inject VERB ct-10670 140 6 , , PUNCT ct-10670 140 7 all all DET ct-10670 140 8 steps step NOUN ct-10670 140 9 are be AUX ct-10670 140 10 repeated repeat VERB ct-10670 140 11 for for ADP ct-10670 140 12 all all DET ct-10670 140 13 oocytes oocyte NOUN ct-10670 140 14 in in ADP ct-10670 140 15 the the DET ct-10670 140 16 dish dish NOUN ct-10670 140 17 ; ; PUNCT ct-10670 140 18 then then ADV ct-10670 140 19 , , PUNCT ct-10670 140 20 they they PRON ct-10670 140 21 can can AUX ct-10670 140 22 be be AUX ct-10670 140 23 moved move VERB ct-10670 140 24 into into ADP ct-10670 140 25 the the DET ct-10670 140 26 embryo embryo NOUN ct-10670 140 27 culture culture NOUN ct-10670 140 28 media medium NOUN ct-10670 140 29 dish dish NOUN ct-10670 140 30 in in ADP ct-10670 140 31 embryo embryo NOUN ct-10670 140 32 culture culture NOUN ct-10670 140 33 medium medium NOUN ct-10670 140 34 ( ( PUNCT ct-10670 140 35 ec ec PROPN ct-10670 140 36 ) ) PUNCT ct-10670 140 37 and and CCONJ ct-10670 140 38 placed place VERB ct-10670 140 39 back back ADV ct-10670 140 40 in in ADP ct-10670 140 41 the the DET ct-10670 140 42 incubator incubator NOUN ct-10670 140 43 . . PUNCT ct-10670 141 1 it it PRON ct-10670 141 2 is be AUX ct-10670 141 3 critical critical ADJ ct-10670 141 4 that that SCONJ ct-10670 141 5 the the DET ct-10670 141 6 injection injection NOUN ct-10670 141 7 itself itself PRON ct-10670 141 8 is be AUX ct-10670 141 9 performed perform VERB ct-10670 141 10 with with ADP ct-10670 141 11 a a DET ct-10670 141 12 sense sense NOUN ct-10670 141 13 of of ADP ct-10670 141 14 urgency urgency NOUN ct-10670 141 15 while while SCONJ ct-10670 141 16 still still ADV ct-10670 141 17 being be AUX ct-10670 141 18 careful careful ADJ ct-10670 141 19 to to PART ct-10670 141 20 not not PART ct-10670 141 21 cause cause VERB ct-10670 141 22 mechanical mechanical ADJ ct-10670 141 23 damage damage NOUN ct-10670 141 24 to to ADP ct-10670 141 25 the the DET ct-10670 141 26 oocyte oocyte NOUN ct-10670 141 27 , , PUNCT ct-10670 141 28 and and CCONJ ct-10670 141 29 the the DET ct-10670 141 30 fertilized fertilize VERB ct-10670 141 31 oocytes oocyte NOUN ct-10670 141 32 be be AUX ct-10670 141 33 placed place VERB ct-10670 141 34 in in ADP ct-10670 141 35 ec ec PROPN ct-10670 141 36 ( ( PUNCT ct-10670 141 37 54 54 NUM ct-10670 141 38 % % NOUN ct-10670 141 39 dmem dmem VERB ct-10670 141 40 / / SYM ct-10670 141 41 f-12 f-12 NOUN ct-10670 141 42 , , PUNCT ct-10670 141 43 40 40 NUM ct-10670 141 44 % % NOUN ct-10670 141 45 global global ADJ ct-10670 141 46 , , PUNCT ct-10670 141 47 6 6 NUM ct-10670 141 48 % % NOUN ct-10670 141 49 fbs fb NOUN ct-10670 141 50 , , PUNCT ct-10670 141 51 10 10 NUM ct-10670 141 52 µl µl NOUN ct-10670 141 53 / / SYM ct-10670 141 54 ml1 ml1 NOUN ct-10670 141 55 its its PRON ct-10670 141 56 solution solution NOUN ct-10670 141 57 , , PUNCT ct-10670 141 58 and and CCONJ ct-10670 141 59 0.1 0.1 NUM ct-10670 141 60 mm mm NOUN ct-10670 141 61 sodium sodium NOUN ct-10670 141 62 pyruvate pyruvate NOUN ct-10670 141 63 ) ) PUNCT ct-10670 141 64 immediately immediately ADV ct-10670 141 65 after after ADP ct-10670 141 66 the the DET ct-10670 141 67 microinjection microinjection NOUN ct-10670 141 68 . . PUNCT ct-10670 142 1 in in ADP ct-10670 142 2 vitro vitro X ct-10670 142 3 culture culture NOUN ct-10670 142 4 of of ADP ct-10670 142 5 equine equine NOUN ct-10670 142 6 embryos embryo NOUN ct-10670 142 7 our our PRON ct-10670 142 8 laboratory laboratory NOUN ct-10670 142 9 uses use VERB ct-10670 142 10 time time NOUN ct-10670 142 11 - - PUNCT ct-10670 142 12 lapse lapse NOUN ct-10670 142 13 embryo embryo NOUN ct-10670 142 14 imaging imaging NOUN ct-10670 142 15 system system NOUN ct-10670 142 16 ( ( PUNCT ct-10670 142 17 miri miri PROPN ct-10670 142 18 ® ® PROPN ct-10670 142 19 tl tl PROPN ct-10670 142 20 , , PUNCT ct-10670 142 21 esco esco PROPN ct-10670 142 22 technologies technology NOUN ct-10670 142 23 ) ) PUNCT ct-10670 142 24 for for ADP ct-10670 142 25 observation observation NOUN ct-10670 142 26 of of ADP ct-10670 142 27 cell cell NOUN ct-10670 142 28 division division NOUN ct-10670 142 29 timing timing NOUN ct-10670 142 30 and and CCONJ ct-10670 142 31 cellular cellular ADJ ct-10670 142 32 integrity integrity NOUN ct-10670 142 33 during during ADP ct-10670 142 34 embryo embryo NOUN ct-10670 142 35 culture culture NOUN ct-10670 142 36 immediately immediately ADV ct-10670 142 37 after after ADP ct-10670 142 38 the the DET ct-10670 142 39 icsi icsi ADJ ct-10670 142 40 procedure procedure NOUN ct-10670 142 41 . . PUNCT ct-10670 143 1 this this DET ct-10670 143 2 noninvasive noninvasive ADJ ct-10670 143 3 system system NOUN ct-10670 143 4 captures capture VERB ct-10670 143 5 bright bright ADJ ct-10670 143 6 - - PUNCT ct-10670 143 7 field field NOUN ct-10670 143 8 images image NOUN ct-10670 143 9 at at ADP ct-10670 143 10 5 5 NUM ct-10670 143 11 - - PUNCT ct-10670 143 12 minutes minute NOUN ct-10670 143 13 intervals interval NOUN ct-10670 143 14 in in ADP ct-10670 143 15 5 5 NUM ct-10670 143 16 various various ADJ ct-10670 143 17 focal focal ADJ ct-10670 143 18 planes plane NOUN ct-10670 143 19 using use VERB ct-10670 143 20 a a DET ct-10670 143 21 single single ADJ ct-10670 143 22 red red ADJ ct-10670 143 23 light light NOUN ct-10670 143 24 - - PUNCT ct-10670 143 25 emitting emit VERB ct-10670 143 26 diode diode NOUN ct-10670 143 27 ( ( PUNCT ct-10670 143 28 635 635 NUM ct-10670 143 29 nm nm NOUN ct-10670 143 30 ) ) PUNCT ct-10670 143 31 , , PUNCT ct-10670 143 32 with with ADP ct-10670 143 33 total total ADJ ct-10670 143 34 light light ADJ ct-10670 143 35 exposure exposure NOUN ct-10670 143 36 of of ADP ct-10670 143 37 0.064 0.064 NUM ct-10670 143 38 second second ADJ ct-10670 143 39 per per ADP ct-10670 143 40 captured capture VERB ct-10670 143 41 image image NOUN ct-10670 143 42 . . PUNCT ct-10670 144 1 figure figure NOUN ct-10670 144 2 3 3 NUM ct-10670 144 3 . . PUNCT ct-10670 145 1 mature mature ADJ ct-10670 145 2 oocyte oocyte NOUN ct-10670 145 3 held hold VERB ct-10670 145 4 by by ADP ct-10670 145 5 holding hold VERB ct-10670 145 6 pipette pipette NOUN ct-10670 145 7 at at ADP ct-10670 145 8 the the DET ct-10670 145 9 9 9 NUM ct-10670 145 10 o’clock o’clock NOUN ct-10670 145 11 position position NOUN ct-10670 145 12 . . PUNCT ct-10670 146 1 first first ADJ ct-10670 146 2 polar polar ADJ ct-10670 146 3 body body NOUN ct-10670 146 4 is be AUX ct-10670 146 5 observed observe VERB ct-10670 146 6 in in ADP ct-10670 146 7 the the DET ct-10670 146 8 bottom bottom ADJ ct-10670 146 9 part part NOUN ct-10670 146 10 of of ADP ct-10670 146 11 the the DET ct-10670 146 12 oocyte oocyte NOUN ct-10670 146 13 ( ( PUNCT ct-10670 146 14 6 6 NUM ct-10670 146 15 o’clock o’clock NOUN ct-10670 146 16 ) ) PUNCT ct-10670 146 17 , , PUNCT ct-10670 146 18 injection injection NOUN ct-10670 146 19 needle needle NOUN ct-10670 146 20 holding hold VERB ct-10670 146 21 a a DET ct-10670 146 22 single single ADJ ct-10670 146 23 sperm sperm NOUN ct-10670 146 24 about about VERB ct-10670 146 25 to to PART ct-10670 146 26 be be AUX ct-10670 146 27 injected inject VERB ct-10670 146 28 in in ADP ct-10670 146 29 the the DET ct-10670 146 30 ooplasm ooplasm NOUN ct-10670 146 31 . . PUNCT ct-10670 147 1 this this DET ct-10670 147 2 image image NOUN ct-10670 147 3 was be AUX ct-10670 147 4 obtained obtain VERB ct-10670 147 5 in in ADP ct-10670 147 6 an an DET ct-10670 147 7 olympus olympus PROPN ct-10670 147 8 microscope microscope NOUN ct-10670 147 9 ( ( PUNCT ct-10670 147 10 ix-70 ix-70 NUM ct-10670 147 11 ) ) PUNCT ct-10670 147 12 20 20 NUM ct-10670 147 13 x x NOUN ct-10670 147 14 objective objective NOUN ct-10670 147 15 . . PUNCT ct-10670 148 1 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 X ct-10670 148 2 6 6 NUM ct-10670 148 3 citation citation NOUN ct-10670 148 4 : : PUNCT ct-10670 148 5 clinical clinical ADJ ct-10670 148 6 theriogenology theriogenology NOUN ct-10670 148 7 2024 2024 NUM ct-10670 148 8 , , PUNCT ct-10670 148 9 16 16 NUM ct-10670 148 10 , , PUNCT ct-10670 148 11 10670 10670 NUM ct-10670 148 12 , , PUNCT ct-10670 148 13 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 X ct-10670 148 14 images image NOUN ct-10670 148 15 are be AUX ct-10670 148 16 then then ADV ct-10670 148 17 assembled assemble VERB ct-10670 148 18 into into ADP ct-10670 148 19 videos video NOUN ct-10670 148 20 that that PRON ct-10670 148 21 can can AUX ct-10670 148 22 be be AUX ct-10670 148 23 used use VERB ct-10670 148 24 for for ADP ct-10670 148 25 retrospective retrospective ADJ ct-10670 148 26 morphokinetic morphokinetic ADJ ct-10670 148 27 analysis analysis NOUN ct-10670 148 28 in in ADP ct-10670 148 29 near near ADP ct-10670 148 30 real real ADJ ct-10670 148 31 - - PUNCT ct-10670 148 32 time time NOUN ct-10670 148 33 . . PUNCT ct-10670 149 1 there there PRON ct-10670 149 2 are be VERB ct-10670 149 3 multiple multiple ADJ ct-10670 149 4 benefits benefit NOUN ct-10670 149 5 to to ADP ct-10670 149 6 time time NOUN ct-10670 149 7 - - PUNCT ct-10670 149 8 lapse lapse NOUN ct-10670 149 9 embryo embryo NOUN ct-10670 149 10 culture culture NOUN ct-10670 149 11 , , PUNCT ct-10670 149 12 but but CCONJ ct-10670 149 13 the the DET ct-10670 149 14 most most ADV ct-10670 149 15 important important ADJ ct-10670 149 16 is be AUX ct-10670 149 17 that that SCONJ ct-10670 149 18 continuous continuous ADJ ct-10670 149 19 embryo embryo NOUN ct-10670 149 20 evaluation evaluation NOUN ct-10670 149 21 is be AUX ct-10670 149 22 possible possible ADJ ct-10670 149 23 without without SCONJ ct-10670 149 24 an an DET ct-10670 149 25 interruption interruption NOUN ct-10670 149 26 to to PART ct-10670 149 27 remove remove VERB ct-10670 149 28 embryos embryo NOUN ct-10670 149 29 from from ADP ct-10670 149 30 the the DET ct-10670 149 31 incubator incubator NOUN ct-10670 149 32 environment environment NOUN ct-10670 149 33 , , PUNCT ct-10670 149 34 except except SCONJ ct-10670 149 35 for for ADP ct-10670 149 36 day day NOUN ct-10670 149 37 4 4 NUM ct-10670 149 38 , , PUNCT ct-10670 149 39 when when SCONJ ct-10670 149 40 the the DET ct-10670 149 41 culture culture NOUN ct-10670 149 42 medium medium NOUN ct-10670 149 43 is be AUX ct-10670 149 44 replaced replace VERB ct-10670 149 45 . . PUNCT ct-10670 150 1 embryos embryo NOUN ct-10670 150 2 are be AUX ct-10670 150 3 vitrified vitrify VERB ct-10670 150 4 when when SCONJ ct-10670 150 5 blastocysts blastocyst NOUN ct-10670 150 6 are be AUX ct-10670 150 7 visualized visualize VERB ct-10670 150 8 to to PART ct-10670 150 9 expand expand VERB ct-10670 150 10 ( ( PUNCT ct-10670 150 11 zona zona NOUN ct-10670 150 12 thinning thinning NOUN ct-10670 150 13 ) ) PUNCT ct-10670 150 14 along along ADP ct-10670 150 15 with with ADP ct-10670 150 16 the the DET ct-10670 150 17 presence presence NOUN ct-10670 150 18 of of ADP ct-10670 150 19 pulsation pulsation NOUN ct-10670 150 20 ( ( PUNCT ct-10670 150 21 see see VERB ct-10670 150 22 below below ADP ct-10670 150 23 description description NOUN ct-10670 150 24 and and CCONJ ct-10670 150 25 video video NOUN ct-10670 150 26 ) ) PUNCT ct-10670 150 27 . . PUNCT ct-10670 151 1 from from ADP ct-10670 151 2 our our PRON ct-10670 151 3 general general ADJ ct-10670 151 4 observations observation NOUN ct-10670 151 5 , , PUNCT ct-10670 151 6 a a DET ct-10670 151 7 normally normally ADV ct-10670 151 8 developing develop VERB ct-10670 151 9 embryo embryo NOUN ct-10670 151 10 is be AUX ct-10670 151 11 expected expect VERB ct-10670 151 12 to to PART ct-10670 151 13 reach reach VERB ct-10670 151 14 the the DET ct-10670 151 15 blastocyst blastocyst NOUN ct-10670 151 16 stage stage NOUN ct-10670 151 17 on on ADP ct-10670 151 18 day day NOUN ct-10670 151 19 7 7 NUM ct-10670 151 20 , , PUNCT ct-10670 151 21 and and CCONJ ct-10670 151 22 embryos embryo NOUN ct-10670 151 23 that that PRON ct-10670 151 24 take take VERB ct-10670 151 25 longer long ADV ct-10670 151 26 have have VERB ct-10670 151 27 lower low ADJ ct-10670 151 28 chances chance NOUN ct-10670 151 29 for for ADP ct-10670 151 30 pregnancy.28 pregnancy.28 NOUN ct-10670 151 31 the the PRON ct-10670 151 32 smaller small ADJ ct-10670 151 33 the the DET ct-10670 151 34 blastocyst blastocyst NOUN ct-10670 151 35 , , PUNCT ct-10670 151 36 the the PRON ct-10670 151 37 higher high ADJ ct-10670 151 38 the the DET ct-10670 151 39 potential potential ADJ ct-10670 151 40 viability viability NOUN ct-10670 151 41 , , PUNCT ct-10670 151 42 thus thus ADV ct-10670 151 43 throughout throughout ADP ct-10670 151 44 their their PRON ct-10670 151 45 monitoring monitoring NOUN ct-10670 151 46 , , PUNCT ct-10670 151 47 we we PRON ct-10670 151 48 make make VERB ct-10670 151 49 sure sure ADJ ct-10670 151 50 to to PART ct-10670 151 51 vitrify vitrify VERB ct-10670 151 52 as as ADV ct-10670 151 53 soon soon ADV ct-10670 151 54 as as ADP ct-10670 151 55 possible possible ADJ ct-10670 151 56 once once SCONJ ct-10670 151 57 a a DET ct-10670 151 58 blastocoel blastocoel NOUN ct-10670 151 59 is be AUX ct-10670 151 60 observed observe VERB ct-10670 151 61 in in ADP ct-10670 151 62 conjunction conjunction NOUN ct-10670 151 63 with with ADP ct-10670 151 64 pulsing pulse VERB ct-10670 151 65 . . PUNCT ct-10670 152 1 our our PRON ct-10670 152 2 laboratory laboratory NOUN ct-10670 152 3 has have AUX ct-10670 152 4 been be AUX ct-10670 152 5 researching research VERB ct-10670 152 6 time time NOUN ct-10670 152 7 - - PUNCT ct-10670 152 8 lapse lapse NOUN ct-10670 152 9 embryo embryo NOUN ct-10670 152 10 evaluation evaluation NOUN ct-10670 152 11 since since SCONJ ct-10670 152 12 2014 2014 NUM ct-10670 152 13 , , PUNCT ct-10670 152 14 and and CCONJ ct-10670 152 15 in in ADP ct-10670 152 16 addition addition NOUN ct-10670 152 17 to to ADP ct-10670 152 18 documenting document VERB ct-10670 152 19 key key ADJ ct-10670 152 20 cell cell NOUN ct-10670 152 21 division division NOUN ct-10670 152 22 and and CCONJ ct-10670 152 23 mitotic mitotic ADJ ct-10670 152 24 events event NOUN ct-10670 152 25 at at ADP ct-10670 152 26 specific specific ADJ ct-10670 152 27 hours hour NOUN ct-10670 152 28 after after ADP ct-10670 152 29 icsi icsi NOUN ct-10670 152 30 a a PRON ct-10670 152 31 b b NOUN ct-10670 152 32 c c X ct-10670 152 33 figure figure NOUN ct-10670 152 34 4 4 NUM ct-10670 152 35 . . PUNCT ct-10670 152 36 cumulus cumulus NOUN ct-10670 152 37 expansion expansion NOUN ct-10670 152 38 during during ADP ct-10670 152 39 the the DET ct-10670 152 40 in in ADV ct-10670 152 41 vitro vitro X ct-10670 152 42 maturation maturation NOUN ct-10670 152 43 culture culture NOUN ct-10670 152 44 for for ADP ct-10670 152 45 27 27 NUM ct-10670 152 46 - - SYM ct-10670 152 47 30 30 NUM ct-10670 152 48 hours hour NOUN ct-10670 152 49 . . PUNCT ct-10670 153 1 a. a. NOUN ct-10670 153 2 initial initial PROPN ct-10670 153 3 state state NOUN ct-10670 153 4 of of ADP ct-10670 153 5 the the DET ct-10670 153 6 cumulus cumulus NOUN ct-10670 153 7 surrounding surround VERB ct-10670 153 8 the the DET ct-10670 153 9 oocyte oocyte NOUN ct-10670 153 10 as as ADP ct-10670 153 11 a a DET ct-10670 153 12 compacted compact VERB ct-10670 153 13 group group NOUN ct-10670 153 14 of of ADP ct-10670 153 15 cells cell NOUN ct-10670 153 16 known know VERB ct-10670 153 17 as as ADP ct-10670 153 18 the the DET ct-10670 153 19 corona corona NOUN ct-10670 153 20 radiata radiata NOUN ct-10670 153 21 ( ( PUNCT ct-10670 153 22 0 0 NUM ct-10670 153 23 hours hour NOUN ct-10670 153 24 ) ) PUNCT ct-10670 153 25 . . PUNCT ct-10670 154 1 b. b. PROPN ct-10670 154 2 cumulus cumulus PROPN ct-10670 154 3 expansion expansion NOUN ct-10670 154 4 at at ADP ct-10670 154 5 mid mid NOUN ct-10670 154 6 - - NOUN ct-10670 154 7 culture culture NOUN ct-10670 154 8 , , PUNCT ct-10670 154 9 or or CCONJ ct-10670 154 10 11.5 11.5 NUM ct-10670 154 11 hours hour NOUN ct-10670 154 12 expansion expansion NOUN ct-10670 154 13 of of ADP ct-10670 154 14 cumulus cumulus NOUN ct-10670 154 15 layer layer NOUN ct-10670 154 16 during during ADP ct-10670 154 17 oocyte oocyte ADJ ct-10670 154 18 maturation maturation NOUN ct-10670 154 19 . . PUNCT ct-10670 155 1 c. c. PROPN ct-10670 155 2 end end NOUN ct-10670 155 3 of of ADP ct-10670 155 4 culture culture NOUN ct-10670 155 5 , , PUNCT ct-10670 155 6 at at ADP ct-10670 155 7 ~ ~ PUNCT ct-10670 155 8 27 27 NUM ct-10670 155 9 hours hour NOUN ct-10670 155 10 , , PUNCT ct-10670 155 11 demonstrates demonstrate VERB ct-10670 155 12 the the DET ct-10670 155 13 maximum maximum ADJ ct-10670 155 14 expansion expansion NOUN ct-10670 155 15 of of ADP ct-10670 155 16 the the DET ct-10670 155 17 cumulus cumulus NOUN ct-10670 155 18 cell cell NOUN ct-10670 155 19 layer layer NOUN ct-10670 155 20 . . PUNCT ct-10670 156 1 images image NOUN ct-10670 156 2 were be AUX ct-10670 156 3 obtained obtain VERB ct-10670 156 4 in in ADP ct-10670 156 5 the the DET ct-10670 156 6 miritl miritl PROPN ct-10670 156 7 ® ® NOUN ct-10670 156 8 time time NOUN ct-10670 156 9 - - PUNCT ct-10670 156 10 lapse lapse NOUN ct-10670 156 11 incubator incubator NOUN ct-10670 156 12 . . PUNCT ct-10670 157 1 a a DET ct-10670 157 2 b b NOUN ct-10670 157 3 c c X ct-10670 157 4 figure figure NOUN ct-10670 157 5 5 5 NUM ct-10670 157 6 . . PUNCT ct-10670 157 7 a. a. NOUN ct-10670 157 8 olympus olympus PROPN ct-10670 157 9 ix-70 ix-70 X ct-10670 157 10 with with ADP ct-10670 157 11 digital digital PROPN ct-10670 157 12 eppendorf eppendorf NOUN ct-10670 157 13 micromanipulators micromanipulator NOUN ct-10670 157 14 and and CCONJ ct-10670 157 15 microinjectors microinjector NOUN ct-10670 157 16 and and CCONJ ct-10670 157 17 brightfield brightfield NOUN ct-10670 157 18 optics optic NOUN ct-10670 157 19 and and CCONJ ct-10670 157 20 heated heated ADJ ct-10670 157 21 stage stage NOUN ct-10670 157 22 . . PUNCT ct-10670 158 1 b. b. PROPN ct-10670 158 2 microscope microscope PROPN ct-10670 158 3 stage stage NOUN ct-10670 158 4 during during ADP ct-10670 158 5 setup setup NOUN ct-10670 158 6 for for ADP ct-10670 158 7 icsi icsi NOUN ct-10670 158 8 with with ADP ct-10670 158 9 injection injection NOUN ct-10670 158 10 dish dish NOUN ct-10670 158 11 under under ADP ct-10670 158 12 sterile sterile ADJ ct-10670 158 13 embryo embryo NOUN ct-10670 158 14 grade grade NOUN ct-10670 158 15 mineral mineral NOUN ct-10670 158 16 oil oil NOUN ct-10670 158 17 . . PUNCT ct-10670 159 1 c. c. NOUN ct-10670 159 2 injection injection NOUN ct-10670 159 3 dish dish NOUN ct-10670 159 4 ready ready ADJ ct-10670 159 5 for for ADP ct-10670 159 6 injection injection NOUN ct-10670 159 7 with with ADP ct-10670 159 8 oocytes oocyte NOUN ct-10670 159 9 in in ADP ct-10670 159 10 g g NOUN ct-10670 159 11 - - PUNCT ct-10670 159 12 mops mop NOUN ct-10670 159 13 medium medium ADJ ct-10670 159 14 , , PUNCT ct-10670 159 15 swim swim NOUN ct-10670 159 16 - - PUNCT ct-10670 159 17 up up ADP ct-10670 159 18 - - PUNCT ct-10670 159 19 selected select VERB ct-10670 159 20 sperm sperm NOUN ct-10670 159 21 in in ADP ct-10670 159 22 7 7 NUM ct-10670 159 23 % % NOUN ct-10670 159 24 pvp pvp NOUN ct-10670 159 25 medium medium NOUN ct-10670 159 26 . . PUNCT ct-10670 160 1 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PROPN ct-10670 160 2 citation citation NOUN ct-10670 160 3 : : PUNCT ct-10670 160 4 clinical clinical ADJ ct-10670 160 5 theriogenology theriogenology NOUN ct-10670 160 6 2024 2024 NUM ct-10670 160 7 , , PUNCT ct-10670 160 8 16 16 NUM ct-10670 160 9 , , PUNCT ct-10670 160 10 10670 10670 NUM ct-10670 160 11 , , PUNCT ct-10670 160 12 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 X ct-10670 160 13 7 7 NUM ct-10670 160 14 ( ( PUNCT ct-10670 160 15 figure figure VERB ct-10670 160 16 6a 6a NOUN ct-10670 160 17 - - PUNCT ct-10670 160 18 f f NOUN ct-10670 160 19 ) ) PUNCT ct-10670 160 20 , , PUNCT ct-10670 160 21 we we PRON ct-10670 160 22 have have AUX ct-10670 160 23 identified identify VERB ct-10670 160 24 several several ADJ ct-10670 160 25 morphokinetic morphokinetic ADJ ct-10670 160 26 parameters parameter NOUN ct-10670 160 27 of of ADP ct-10670 160 28 successful successful ADJ ct-10670 160 29 blastocysts blastocyst NOUN ct-10670 160 30 that that PRON ct-10670 160 31 are be AUX ct-10670 160 32 consistent consistent ADJ ct-10670 160 33 with with ADP ct-10670 160 34 blastocyst blastocyst NOUN ct-10670 160 35 formation formation NOUN ct-10670 160 36 and and CCONJ ct-10670 160 37 pregnancy pregnancy NOUN ct-10670 160 38 success success NOUN ct-10670 160 39 rates.13,29 rates.13,29 NOUN ct-10670 160 40 we we PRON ct-10670 160 41 have have AUX ct-10670 160 42 described describe VERB ct-10670 160 43 2 2 NUM ct-10670 160 44 cleavage cleavage NOUN ct-10670 160 45 - - PUNCT ct-10670 160 46 related relate VERB ct-10670 160 47 phenomena phenomenon NOUN ct-10670 160 48 that that PRON ct-10670 160 49 are be AUX ct-10670 160 50 closely closely ADV ct-10670 160 51 associated associate VERB ct-10670 160 52 with with ADP ct-10670 160 53 the the DET ct-10670 160 54 completion completion NOUN ct-10670 160 55 of of ADP ct-10670 160 56 development development NOUN ct-10670 160 57 to to ADP ct-10670 160 58 a a DET ct-10670 160 59 blastocyst blastocyst NOUN ct-10670 160 60 . . PUNCT ct-10670 161 1 first first ADV ct-10670 161 2 , , PUNCT ct-10670 161 3 a a DET ct-10670 161 4 precleavage precleavage NOUN ct-10670 161 5 event event NOUN ct-10670 161 6 known know VERB ct-10670 161 7 as as ADP ct-10670 161 8 cytoplasmic cytoplasmic ADJ ct-10670 161 9 extrusion extrusion NOUN ct-10670 161 10 occurs occur VERB ct-10670 161 11 in in ADP ct-10670 161 12 equine equine NOUN ct-10670 161 13 zygotes zygote NOUN ct-10670 161 14 , , PUNCT ct-10670 161 15 whereby whereby SCONJ ct-10670 161 16 extraneous extraneous ADJ ct-10670 161 17 cytoplasmic cytoplasmic ADJ ct-10670 161 18 zygotic zygotic ADJ ct-10670 161 19 material material NOUN ct-10670 161 20 is be AUX ct-10670 161 21 released release VERB ct-10670 161 22 into into ADP ct-10670 161 23 the the DET ct-10670 161 24 perivitelline perivitelline ADJ ct-10670 161 25 space space NOUN ct-10670 161 26 from from ADP ct-10670 161 27 the the DET ct-10670 161 28 single single ADJ ct-10670 161 29 - - PUNCT ct-10670 161 30 celled celle VERB ct-10670 161 31 zygote zygote NOUN ct-10670 161 32 just just ADV ct-10670 161 33 before before ADP ct-10670 161 34 cleavage cleavage NOUN ct-10670 161 35 division division NOUN ct-10670 161 36 ( ( PUNCT ct-10670 161 37 figure figure NOUN ct-10670 161 38 6b 6b NUM ct-10670 161 39 ) ) PUNCT ct-10670 161 40 . . PUNCT ct-10670 162 1 embryos embryo NOUN ct-10670 162 2 that that PRON ct-10670 162 3 fail fail VERB ct-10670 162 4 to to PART ct-10670 162 5 become become VERB ct-10670 162 6 blastocysts blastocyst NOUN ct-10670 162 7 have have VERB ct-10670 162 8 a a DET ct-10670 162 9 lower low ADJ ct-10670 162 10 rate rate NOUN ct-10670 162 11 of of ADP ct-10670 162 12 this this DET ct-10670 162 13 precleavage precleavage NOUN ct-10670 162 14 extrusion.13 extrusion.13 X ct-10670 162 15 second second ADJ ct-10670 162 16 , , PUNCT ct-10670 162 17 as as SCONJ ct-10670 162 18 the the DET ct-10670 162 19 embryo embryo NOUN ct-10670 162 20 reaches reach VERB ct-10670 162 21 the the DET ct-10670 162 22 late late ADJ ct-10670 162 23 morula morula NOUN ct-10670 162 24 and and CCONJ ct-10670 162 25 early early ADJ ct-10670 162 26 blastocyst blastocyst NOUN ct-10670 162 27 stage stage NOUN ct-10670 162 28 , , PUNCT ct-10670 162 29 we we PRON ct-10670 162 30 routinely routinely ADV ct-10670 162 31 observe observe VERB ct-10670 162 32 the the DET ct-10670 162 33 successful successful ADJ ct-10670 162 34 embryo embryo NOUN ct-10670 162 35 begin begin VERB ct-10670 162 36 a a DET ct-10670 162 37 pulsation pulsation NOUN ct-10670 162 38 sequence30 sequence30 NOUN ct-10670 162 39 at at ADP ct-10670 162 40 regular regular ADJ ct-10670 162 41 intervals interval NOUN ct-10670 162 42 ( ( PUNCT ct-10670 162 43 0.22 0.22 NUM ct-10670 162 44 hour hour NOUN ct-10670 162 45 ) ) PUNCT ct-10670 162 46 , , PUNCT ct-10670 162 47 in in ADP ct-10670 162 48 which which PRON ct-10670 162 49 an an DET ct-10670 162 50 overall overall ADJ ct-10670 162 51 growth growth NOUN ct-10670 162 52 in in ADP ct-10670 162 53 embryo embryo NOUN ct-10670 162 54 diameter diameter NOUN ct-10670 162 55 occurs occur VERB ct-10670 162 56 with with ADP ct-10670 162 57 expansion expansion NOUN ct-10670 162 58 and and CCONJ ct-10670 162 59 subsequent subsequent ADJ ct-10670 162 60 contraction contraction NOUN ct-10670 162 61 . . PUNCT ct-10670 163 1 the the DET ct-10670 163 2 pulsation pulsation NOUN ct-10670 163 3 also also ADV ct-10670 163 4 results result VERB ct-10670 163 5 in in ADP ct-10670 163 6 thinning thinning NOUN ct-10670 163 7 of of ADP ct-10670 163 8 the the DET ct-10670 163 9 zona zona PROPN ct-10670 163 10 pellucida pellucida PROPN ct-10670 163 11 and and CCONJ ct-10670 163 12 can can AUX ct-10670 163 13 only only ADV ct-10670 163 14 be be AUX ct-10670 163 15 observed observe VERB ct-10670 163 16 using use VERB ct-10670 163 17 the the DET ct-10670 163 18 time time NOUN ct-10670 163 19 - - PUNCT ct-10670 163 20 lapse lapse NOUN ct-10670 163 21 videos video NOUN ct-10670 163 22 rather rather ADV ct-10670 163 23 than than ADP ct-10670 163 24 single single ADJ ct-10670 163 25 observations observation NOUN ct-10670 163 26 . . PUNCT ct-10670 164 1 we we PRON ct-10670 164 2 have have AUX ct-10670 164 3 reported report VERB ct-10670 164 4 this this DET ct-10670 164 5 dynamic dynamic ADJ ct-10670 164 6 phenomenon phenomenon NOUN ct-10670 164 7 to to PART ct-10670 164 8 occur occur VERB ct-10670 164 9 in in ADP ct-10670 164 10 both both CCONJ ct-10670 164 11 in in ADP ct-10670 164 12 vitro vitro X ct-10670 164 13 and and CCONJ ct-10670 164 14 in in ADP ct-10670 164 15 vivo vivo ADJ ct-10670 164 16 derived derive VERB ct-10670 164 17 embryos embryo NOUN ct-10670 164 18 ( ( PUNCT ct-10670 164 19 flushed flush VERB ct-10670 164 20 from from ADP ct-10670 164 21 the the DET ct-10670 164 22 uterus uterus NOUN ct-10670 164 23 of of ADP ct-10670 164 24 mares mare NOUN ct-10670 164 25 at at ADP ct-10670 164 26 6.5 6.5 NUM ct-10670 164 27 days day NOUN ct-10670 164 28 postovulation postovulation NOUN ct-10670 164 29 and and CCONJ ct-10670 164 30 cultured cultured ADJ ct-10670 164 31 in in ADP ct-10670 164 32 the the DET ct-10670 164 33 tl tl PROPN ct-10670 164 34 system).30 system).30 PUNCT ct-10670 164 35 while while SCONJ ct-10670 164 36 irregularities irregularity NOUN ct-10670 164 37 in in ADP ct-10670 164 38 mitosis mitosis NOUN ct-10670 164 39 can can AUX ct-10670 164 40 figure figure VERB ct-10670 164 41 6 6 NUM ct-10670 164 42 . . PUNCT ct-10670 164 43 time time NOUN ct-10670 164 44 - - PUNCT ct-10670 164 45 lapse lapse NOUN ct-10670 164 46 development development NOUN ct-10670 164 47 in in ADP ct-10670 164 48 an an DET ct-10670 164 49 equine equine NOUN ct-10670 164 50 zygote zygote NOUN ct-10670 164 51 after after ADP ct-10670 164 52 icsi icsi NOUN ct-10670 164 53 ( ( PUNCT ct-10670 164 54 a a NOUN ct-10670 164 55 ) ) PUNCT ct-10670 164 56 and and CCONJ ct-10670 164 57 progressing progress VERB ct-10670 164 58 to to ADP ct-10670 164 59 blastocyst blastocyst NOUN ct-10670 164 60 stage stage NOUN ct-10670 164 61 ( ( PUNCT ct-10670 164 62 b b X ct-10670 164 63 - - SYM ct-10670 164 64 f f NOUN ct-10670 164 65 ) ) PUNCT ct-10670 164 66 as as SCONJ ct-10670 164 67 visualized visualize VERB ct-10670 164 68 in in ADP ct-10670 164 69 the the DET ct-10670 164 70 miritl miritl PROPN ct-10670 164 71 ® ® NOUN ct-10670 164 72 incubator incubator NOUN ct-10670 164 73 . . PUNCT ct-10670 165 1 a. a. NOUN ct-10670 165 2 equine equine PROPN ct-10670 165 3 oocyte oocyte PROPN ct-10670 165 4 immediately immediately ADV ct-10670 165 5 after after ADP ct-10670 165 6 sperm sperm NOUN ct-10670 165 7 injection injection NOUN ct-10670 165 8 and and CCONJ ct-10670 165 9 placement placement NOUN ct-10670 165 10 into into ADP ct-10670 165 11 time time NOUN ct-10670 165 12 - - PUNCT ct-10670 165 13 lapse lapse NOUN ct-10670 165 14 incubator incubator NOUN ct-10670 165 15 . . PUNCT ct-10670 166 1 we we PRON ct-10670 166 2 observe observe VERB ct-10670 166 3 cellular cellular ADJ ct-10670 166 4 extrusion extrusion NOUN ct-10670 166 5 ( ( PUNCT ct-10670 166 6 b b NOUN ct-10670 166 7 ) ) PUNCT ct-10670 166 8 to to PART ct-10670 166 9 occur occur VERB ct-10670 166 10 soon soon ADV ct-10670 166 11 before before ADP ct-10670 166 12 the the DET ct-10670 166 13 first first ADJ ct-10670 166 14 cleavage cleavage NOUN ct-10670 166 15 where where SCONJ ct-10670 166 16 the the DET ct-10670 166 17 extruded extrude VERB ct-10670 166 18 material material NOUN ct-10670 166 19 occupies occupy VERB ct-10670 166 20 the the DET ct-10670 166 21 perivitelline perivitelline ADJ ct-10670 166 22 space space NOUN ct-10670 166 23 . . PUNCT ct-10670 167 1 in in ADP ct-10670 167 2 ( ( PUNCT ct-10670 167 3 c c NOUN ct-10670 167 4 ) ) PUNCT ct-10670 167 5 , , PUNCT ct-10670 167 6 2 2 NUM ct-10670 167 7 blastomeres blastomere NOUN ct-10670 167 8 are be AUX ct-10670 167 9 visualized visualize VERB ct-10670 167 10 alongside alongside ADP ct-10670 167 11 the the DET ct-10670 167 12 extruded extrude VERB ct-10670 167 13 material material NOUN ct-10670 167 14 , , PUNCT ct-10670 167 15 giving give VERB ct-10670 167 16 an an DET ct-10670 167 17 impression impression NOUN ct-10670 167 18 of of ADP ct-10670 167 19 a a DET ct-10670 167 20 third third ADJ ct-10670 167 21 blastomere blastomere NOUN ct-10670 167 22 . . PUNCT ct-10670 168 1 d. d. NOUN ct-10670 168 2 formation formation NOUN ct-10670 168 3 of of ADP ct-10670 168 4 a a DET ct-10670 168 5 4 4 NUM ct-10670 168 6 - - PUNCT ct-10670 168 7 cell cell NOUN ct-10670 168 8 embryo embryo NOUN ct-10670 168 9 . . PUNCT ct-10670 169 1 however however ADV ct-10670 169 2 , , PUNCT ct-10670 169 3 in in ADP ct-10670 169 4 contrast contrast NOUN ct-10670 169 5 to to ADP ct-10670 169 6 human human ADJ ct-10670 169 7 embryos embryo NOUN ct-10670 169 8 , , PUNCT ct-10670 169 9 it it PRON ct-10670 169 10 quickly quickly ADV ct-10670 169 11 becomes become VERB ct-10670 169 12 impossible impossible ADJ ct-10670 169 13 to to PART ct-10670 169 14 judge judge VERB ct-10670 169 15 the the DET ct-10670 169 16 number number NOUN ct-10670 169 17 of of ADP ct-10670 169 18 blastomeres blastomere NOUN ct-10670 169 19 beyond beyond ADP ct-10670 169 20 the the DET ct-10670 169 21 8 8 NUM ct-10670 169 22 - - PUNCT ct-10670 169 23 cell cell NOUN ct-10670 169 24 stage stage NOUN ct-10670 169 25 due due ADP ct-10670 169 26 to to ADP ct-10670 169 27 the the DET ct-10670 169 28 equine equine NOUN ct-10670 169 29 blastomeres blastomere NOUN ct-10670 169 30 ’ ’ PART ct-10670 169 31 dark dark ADJ ct-10670 169 32 color color NOUN ct-10670 169 33 ( ( PUNCT ct-10670 169 34 e e NOUN ct-10670 169 35 , , PUNCT ct-10670 169 36 f f NOUN ct-10670 169 37 ) ) PUNCT ct-10670 169 38 . . PUNCT ct-10670 170 1 f. f. PROPN ct-10670 170 2 icsi icsi PROPN ct-10670 170 3 - - PUNCT ct-10670 170 4 derived derive VERB ct-10670 170 5 blastocyst blastocyst NOUN ct-10670 170 6 . . PUNCT ct-10670 171 1 video video NOUN ct-10670 171 2 . . PUNCT ct-10670 172 1 time time NOUN ct-10670 172 2 - - PUNCT ct-10670 172 3 lapse lapse NOUN ct-10670 172 4 development development NOUN ct-10670 172 5 in in ADP ct-10670 172 6 an an DET ct-10670 172 7 equine equine NOUN ct-10670 172 8 early early ADJ ct-10670 172 9 embryo embryo NOUN ct-10670 172 10 . . PUNCT ct-10670 173 1 to to PART ct-10670 173 2 view view VERB ct-10670 173 3 the the DET ct-10670 173 4 video video NOUN ct-10670 173 5 , , PUNCT ct-10670 173 6 read read VERB ct-10670 173 7 the the DET ct-10670 173 8 html html NOUN ct-10670 173 9 version version NOUN ct-10670 173 10 of of ADP ct-10670 173 11 the the DET ct-10670 173 12 article article NOUN ct-10670 173 13 where where SCONJ ct-10670 173 14 the the DET ct-10670 173 15 video video NOUN ct-10670 173 16 is be AUX ct-10670 173 17 embedded embed VERB ct-10670 173 18 . . PUNCT ct-10670 174 1 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PROPN ct-10670 174 2 8 8 NUM ct-10670 174 3 citation citation NOUN ct-10670 174 4 : : PUNCT ct-10670 174 5 clinical clinical ADJ ct-10670 174 6 theriogenology theriogenology NOUN ct-10670 174 7 2024 2024 NUM ct-10670 174 8 , , PUNCT ct-10670 174 9 16 16 NUM ct-10670 174 10 , , PUNCT ct-10670 174 11 10670 10670 NUM ct-10670 174 12 , , PUNCT ct-10670 174 13 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PRON ct-10670 174 14 often often ADV ct-10670 174 15 indicate indicate VERB ct-10670 174 16 aneuploidy aneuploidy ADJ ct-10670 174 17 or or CCONJ ct-10670 174 18 polyploidy polyploidy ADJ ct-10670 174 19 in in ADP ct-10670 174 20 developing develop VERB ct-10670 174 21 embryos embryo NOUN ct-10670 174 22 , , PUNCT ct-10670 174 23 embryo embryo NOUN ct-10670 174 24 pulsing pulsing NOUN ct-10670 174 25 is be AUX ct-10670 174 26 linked link VERB ct-10670 174 27 to to ADP ct-10670 174 28 successful successful ADJ ct-10670 174 29 implantation implantation NOUN ct-10670 174 30 in in ADP ct-10670 174 31 human human ADJ ct-10670 174 32 embryos.31,32 embryos.31,32 NOUN ct-10670 174 33 our our PRON ct-10670 174 34 observations observation NOUN ct-10670 174 35 are be AUX ct-10670 174 36 in in ADP ct-10670 174 37 agreement agreement NOUN ct-10670 174 38 with with ADP ct-10670 174 39 these these DET ct-10670 174 40 reports.29,30 reports.29,30 NOUN ct-10670 174 41 the the DET ct-10670 174 42 tl tl PROPN ct-10670 174 43 system system NOUN ct-10670 174 44 has have AUX ct-10670 174 45 allowed allow VERB ct-10670 174 46 researchers researcher NOUN ct-10670 174 47 to to PART ct-10670 174 48 document document VERB ct-10670 174 49 the the DET ct-10670 174 50 embryo embryo NOUN ct-10670 174 51 development development NOUN ct-10670 174 52 timeline timeline NOUN ct-10670 174 53 for for ADP ct-10670 174 54 each each DET ct-10670 174 55 cell cell NOUN ct-10670 174 56 division division NOUN ct-10670 174 57 of of ADP ct-10670 174 58 growing grow VERB ct-10670 174 59 human human ADJ ct-10670 174 60 , , PUNCT ct-10670 174 61 bovine bovine NOUN ct-10670 174 62 , , PUNCT ct-10670 174 63 and and CCONJ ct-10670 174 64 equine equine VERB ct-10670 174 65 embryos.13,31,33 embryos.13,31,33 NOUN ct-10670 174 66 - - NOUN ct-10670 174 67 35 35 NUM ct-10670 174 68 as as SCONJ ct-10670 174 69 mentioned mention VERB ct-10670 174 70 above above ADV ct-10670 174 71 , , PUNCT ct-10670 174 72 studies study NOUN ct-10670 174 73 from from ADP ct-10670 174 74 our our PRON ct-10670 174 75 laboratory laboratory NOUN ct-10670 174 76 and and CCONJ ct-10670 174 77 others other NOUN ct-10670 174 78 have have AUX ct-10670 174 79 determined determine VERB ct-10670 174 80 the the DET ct-10670 174 81 precise precise ADJ ct-10670 174 82 timing timing NOUN ct-10670 174 83 of of ADP ct-10670 174 84 embryo embryo NOUN ct-10670 174 85 cleavage cleavage NOUN ct-10670 174 86 from from ADP ct-10670 174 87 a a DET ct-10670 174 88 single single ADJ ct-10670 174 89 cell cell NOUN ct-10670 174 90 fertilized fertilize VERB ct-10670 174 91 zygote zygote NOUN ct-10670 174 92 from from ADP ct-10670 174 93 the the DET ct-10670 174 94 moment moment NOUN ct-10670 174 95 of of ADP ct-10670 174 96 sperm sperm NOUN ct-10670 174 97 injection injection NOUN ct-10670 174 98 to to ADP ct-10670 174 99 2 2 NUM ct-10670 174 100 - - PUNCT ct-10670 174 101 cell cell NOUN ct-10670 174 102 , , PUNCT ct-10670 174 103 4 4 NUM ct-10670 174 104 - - PUNCT ct-10670 174 105 cell cell NOUN ct-10670 174 106 , , PUNCT ct-10670 174 107 8 8 NUM ct-10670 174 108 - - PUNCT ct-10670 174 109 cell cell NOUN ct-10670 174 110 , , PUNCT ct-10670 174 111 morula morula NOUN ct-10670 174 112 and and CCONJ ct-10670 174 113 blastocyst.13 blastocyst.13 VERB ct-10670 174 114 recent recent ADJ ct-10670 174 115 work work NOUN ct-10670 174 116 from from ADP ct-10670 174 117 our our PRON ct-10670 174 118 laboratory laboratory NOUN ct-10670 174 119 has have AUX ct-10670 174 120 extended extend VERB ct-10670 174 121 the the DET ct-10670 174 122 observations observation NOUN ct-10670 174 123 of of ADP ct-10670 174 124 the the DET ct-10670 174 125 first first ADJ ct-10670 174 126 few few ADJ ct-10670 174 127 equine equine NOUN ct-10670 174 128 cell cell NOUN ct-10670 174 129 divisions division NOUN ct-10670 174 130 and and CCONJ ct-10670 174 131 their their PRON ct-10670 174 132 influence influence NOUN ct-10670 174 133 on on ADP ct-10670 174 134 blastocyst blastocyst NOUN ct-10670 174 135 development development NOUN ct-10670 174 136 to to ADP ct-10670 174 137 that that PRON ct-10670 174 138 of of ADP ct-10670 174 139 equine equine NOUN ct-10670 174 140 pregnancy pregnancy NOUN ct-10670 174 141 outcomes outcome NOUN ct-10670 174 142 . . PUNCT ct-10670 175 1 with with ADP ct-10670 175 2 ~ ~ SYM ct-10670 175 3 70 70 NUM ct-10670 175 4 pregnancies pregnancy NOUN ct-10670 175 5 from from ADP ct-10670 175 6 transferred transfer VERB ct-10670 175 7 vitrified vitrified ADJ ct-10670 175 8 blastocysts blastocyst NOUN ct-10670 175 9 , , PUNCT ct-10670 175 10 we we PRON ct-10670 175 11 observed observe VERB ct-10670 175 12 that that SCONJ ct-10670 175 13 those those PRON ct-10670 175 14 stemming stem VERB ct-10670 175 15 from from ADP ct-10670 175 16 embryos embryo NOUN ct-10670 175 17 with with ADP ct-10670 175 18 low low ADJ ct-10670 175 19 rates rate NOUN ct-10670 175 20 of of ADP ct-10670 175 21 abnormal abnormal ADJ ct-10670 175 22 early early ADJ ct-10670 175 23 - - PUNCT ct-10670 175 24 stage stage NOUN ct-10670 175 25 cleavages cleavage NOUN ct-10670 175 26 ( ( PUNCT ct-10670 175 27 at at ADP ct-10670 175 28 the the DET ct-10670 175 29 2 2 NUM ct-10670 175 30 - - PUNCT ct-10670 175 31 cell cell NOUN ct-10670 175 32 stage stage NOUN ct-10670 175 33 ) ) PUNCT ct-10670 175 34 were be AUX ct-10670 175 35 sustained sustain VERB ct-10670 175 36 at at ADP ct-10670 175 37 a a DET ct-10670 175 38 higher high ADJ ct-10670 175 39 percentage percentage NOUN ct-10670 175 40 . . PUNCT ct-10670 176 1 these these DET ct-10670 176 2 pregnancies pregnancy NOUN ct-10670 176 3 demonstrated demonstrate VERB ct-10670 176 4 a a DET ct-10670 176 5 lower low ADJ ct-10670 176 6 rate rate NOUN ct-10670 176 7 of of ADP ct-10670 176 8 pregnancy pregnancy NOUN ct-10670 176 9 loss loss NOUN ct-10670 176 10 further far ADV ct-10670 176 11 on on ADV ct-10670 176 12 during during ADP ct-10670 176 13 pregnancy pregnancy NOUN ct-10670 176 14 demonstrating demonstrating NOUN ct-10670 176 15 that that SCONJ ct-10670 176 16 early early ADJ ct-10670 176 17 cleavage cleavage NOUN ct-10670 176 18 abnormalities abnormality NOUN ct-10670 176 19 likely likely ADV ct-10670 176 20 lead lead VERB ct-10670 176 21 to to ADP ct-10670 176 22 subsequent subsequent ADJ ct-10670 176 23 pregnancy pregnancy NOUN ct-10670 176 24 loss.28 loss.28 NOUN ct-10670 176 25 we we PRON ct-10670 176 26 can can AUX ct-10670 176 27 not not PART ct-10670 176 28 , , PUNCT ct-10670 176 29 at at ADP ct-10670 176 30 this this DET ct-10670 176 31 point point NOUN ct-10670 176 32 , , PUNCT ct-10670 176 33 determine determine VERB ct-10670 176 34 the the DET ct-10670 176 35 cost cost NOUN ct-10670 176 36 - - PUNCT ct-10670 176 37 effectiveness effectiveness NOUN ct-10670 176 38 of of ADP ct-10670 176 39 the the DET ct-10670 176 40 time time NOUN ct-10670 176 41 - - PUNCT ct-10670 176 42 lapse lapse NOUN ct-10670 176 43 system system NOUN ct-10670 176 44 as as SCONJ ct-10670 176 45 this this DET ct-10670 176 46 new new ADJ ct-10670 176 47 technology technology NOUN ct-10670 176 48 for for ADP ct-10670 176 49 equine equine NOUN ct-10670 176 50 development development NOUN ct-10670 176 51 has have AUX ct-10670 176 52 not not PART ct-10670 176 53 been be AUX ct-10670 176 54 fully fully ADV ct-10670 176 55 evaluated evaluate VERB ct-10670 176 56 for for ADP ct-10670 176 57 adequate adequate ADJ ct-10670 176 58 numbers number NOUN ct-10670 176 59 of of ADP ct-10670 176 60 clinical clinical ADJ ct-10670 176 61 pregnancy pregnancy NOUN ct-10670 176 62 outcomes outcome NOUN ct-10670 176 63 . . PUNCT ct-10670 177 1 blastocyst blastocyst NOUN ct-10670 177 2 vitrification vitrification NOUN ct-10670 177 3 and and CCONJ ct-10670 177 4 thawing thaw VERB ct-10670 177 5 for for ADP ct-10670 177 6 embryo embryo NOUN ct-10670 177 7 transfer transfer NOUN ct-10670 177 8 a a DET ct-10670 177 9 great great ADJ ct-10670 177 10 majority majority NOUN ct-10670 177 11 of of ADP ct-10670 177 12 the the DET ct-10670 177 13 blastocysts blastocyst NOUN ct-10670 177 14 produced produce VERB ct-10670 177 15 in in ADP ct-10670 177 16 our our PRON ct-10670 177 17 laboratory laboratory NOUN ct-10670 177 18 are be AUX ct-10670 177 19 vitrified vitrify VERB ct-10670 177 20 and and CCONJ ct-10670 177 21 stored store VERB ct-10670 177 22 in in ADP ct-10670 177 23 liquid liquid ADJ ct-10670 177 24 nitrogen nitrogen NOUN ct-10670 177 25 or or CCONJ ct-10670 177 26 shipped ship VERB ct-10670 177 27 to to ADP ct-10670 177 28 other other ADJ ct-10670 177 29 clinics clinic NOUN ct-10670 177 30 . . PUNCT ct-10670 178 1 embryos embryo NOUN ct-10670 178 2 are be AUX ct-10670 178 3 vitrified vitrify VERB ct-10670 178 4 as as ADV ct-10670 178 5 soon soon ADV ct-10670 178 6 as as SCONJ ct-10670 178 7 they they PRON ct-10670 178 8 reach reach VERB ct-10670 178 9 the the DET ct-10670 178 10 blastocyst blastocyst NOUN ct-10670 178 11 stage stage NOUN ct-10670 178 12 ( ( PUNCT ct-10670 178 13 i.e. i.e. X ct-10670 178 14 a a DET ct-10670 178 15 clear clear ADJ ct-10670 178 16 trophectoderm trophectoderm NOUN ct-10670 178 17 and and CCONJ ct-10670 178 18 blastocoele blastocoele PROPN ct-10670 178 19 structures structure NOUN ct-10670 178 20 , , PUNCT ct-10670 178 21 and and CCONJ ct-10670 178 22 pulsation pulsation NOUN ct-10670 178 23 can can AUX ct-10670 178 24 be be AUX ct-10670 178 25 observed observe VERB ct-10670 178 26 ) ) PUNCT ct-10670 178 27 , , PUNCT ct-10670 178 28 particularly particularly ADV ct-10670 178 29 if if SCONJ ct-10670 178 30 no no DET ct-10670 178 31 recipient recipient NOUN ct-10670 178 32 mares mare NOUN ct-10670 178 33 are be AUX ct-10670 178 34 available available ADJ ct-10670 178 35 or or CCONJ ct-10670 178 36 the the DET ct-10670 178 37 client client NOUN ct-10670 178 38 desires desire NOUN ct-10670 178 39 storage storage NOUN ct-10670 178 40 of of ADP ct-10670 178 41 the the DET ct-10670 178 42 embryos embryo NOUN ct-10670 178 43 for for ADP ct-10670 178 44 a a DET ct-10670 178 45 later later ADJ ct-10670 178 46 transfer transfer NOUN ct-10670 178 47 . . PUNCT ct-10670 179 1 we we PRON ct-10670 179 2 use use VERB ct-10670 179 3 a a DET ct-10670 179 4 2 2 NUM ct-10670 179 5 - - PUNCT ct-10670 179 6 step step NOUN ct-10670 179 7 vitrification vitrification NOUN ct-10670 179 8 protocol protocol NOUN ct-10670 179 9 with with ADP ct-10670 179 10 1.5 1.5 NUM ct-10670 179 11 m m NOUN ct-10670 179 12 ethylene ethylene NOUN ct-10670 179 13 glycol glycol NOUN ct-10670 179 14 ( ( PUNCT ct-10670 179 15 solution solution NOUN ct-10670 179 16 a a NOUN ct-10670 179 17 ) ) PUNCT ct-10670 179 18 and and CCONJ ct-10670 179 19 7.0 7.0 NUM ct-10670 179 20 m m PROPN ct-10670 179 21 ethylene ethylene NOUN ct-10670 179 22 glycol glycol NOUN ct-10670 179 23 , , PUNCT ct-10670 179 24 and and CCONJ ct-10670 179 25 0.6 0.6 NUM ct-10670 179 26 m m NOUN ct-10670 179 27 galactose galactose NOUN ct-10670 179 28 ( ( PUNCT ct-10670 179 29 solution solution NOUN ct-10670 179 30 b b NOUN ct-10670 179 31 ) ) PUNCT ct-10670 179 32 . . PUNCT ct-10670 180 1 cryolock cryolock VERB ct-10670 180 2 ® ® NOUN ct-10670 180 3 vitrification vitrification NOUN ct-10670 180 4 devices device NOUN ct-10670 180 5 ( ( PUNCT ct-10670 180 6 biotech biotech PROPN ct-10670 180 7 , , PUNCT ct-10670 180 8 inc inc PROPN ct-10670 180 9 , , PUNCT ct-10670 180 10 alpharetta alpharetta PROPN ct-10670 180 11 , , PUNCT ct-10670 180 12 ga ga PROPN ct-10670 180 13 ) ) PUNCT ct-10670 180 14 are be AUX ct-10670 180 15 used use VERB ct-10670 180 16 to to PART ct-10670 180 17 store store VERB ct-10670 180 18 the the DET ct-10670 180 19 frozen frozen ADJ ct-10670 180 20 blastocysts blastocyst NOUN ct-10670 180 21 which which PRON ct-10670 180 22 are be AUX ct-10670 180 23 then then ADV ct-10670 180 24 stored store VERB ct-10670 180 25 in in ADP ct-10670 180 26 individual individual ADJ ct-10670 180 27 canes cane NOUN ct-10670 180 28 in in ADP ct-10670 180 29 liquid liquid ADJ ct-10670 180 30 nitrogen nitrogen NOUN ct-10670 180 31 dewars dewar NOUN ct-10670 180 32 . . PUNCT ct-10670 181 1 vitrified vitrify VERB ct-10670 181 2 embryos embryo NOUN ct-10670 181 3 , , PUNCT ct-10670 181 4 shipped ship VERB ct-10670 181 5 overnight overnight ADV ct-10670 181 6 in in ADP ct-10670 181 7 dry dry ADJ ct-10670 181 8 shipping shipping NOUN ct-10670 181 9 liquid liquid ADJ ct-10670 181 10 nitrogen nitrogen NOUN ct-10670 181 11 containers container NOUN ct-10670 181 12 , , PUNCT ct-10670 181 13 are be AUX ct-10670 181 14 thawed thaw VERB ct-10670 181 15 and and CCONJ ct-10670 181 16 warmed warm VERB ct-10670 181 17 after after ADP ct-10670 181 18 a a DET ct-10670 181 19 simple simple ADJ ct-10670 181 20 1 1 NUM ct-10670 181 21 - - PUNCT ct-10670 181 22 step step NOUN ct-10670 181 23 , , PUNCT ct-10670 181 24 but but CCONJ ct-10670 181 25 effective effective ADJ ct-10670 181 26 , , PUNCT ct-10670 181 27 field field NOUN ct-10670 181 28 protocol protocol NOUN ct-10670 181 29 , , PUNCT ct-10670 181 30 which which PRON ct-10670 181 31 only only ADV ct-10670 181 32 requires require VERB ct-10670 181 33 the the DET ct-10670 181 34 use use NOUN ct-10670 181 35 of of ADP ct-10670 181 36 a a DET ct-10670 181 37 commercial commercial ADJ ct-10670 181 38 holding hold VERB ct-10670 181 39 media medium NOUN ct-10670 181 40 by by ADP ct-10670 181 41 veterinarians veterinarian NOUN ct-10670 181 42 in in ADP ct-10670 181 43 the the DET ct-10670 181 44 field field NOUN ct-10670 181 45 . . PUNCT ct-10670 182 1 a a DET ct-10670 182 2 sterile sterile ADJ ct-10670 182 3 , , PUNCT ct-10670 182 4 30 30 NUM ct-10670 182 5 - - PUNCT ct-10670 182 6 mm mm NOUN ct-10670 182 7 culture culture NOUN ct-10670 182 8 dish dish NOUN ct-10670 182 9 is be AUX ct-10670 182 10 filled fill VERB ct-10670 182 11 with with ADP ct-10670 182 12 room room NOUN ct-10670 182 13 temperature temperature NOUN ct-10670 182 14 holding hold VERB ct-10670 182 15 media medium NOUN ct-10670 182 16 and and CCONJ ct-10670 182 17 the the DET ct-10670 182 18 cryolock cryolock NOUN ct-10670 182 19 ® ® NOUN ct-10670 182 20 is be AUX ct-10670 182 21 quickly quickly ADV ct-10670 182 22 moved move VERB ct-10670 182 23 from from ADP ct-10670 182 24 liquid liquid ADJ ct-10670 182 25 nitrogen nitrogen NOUN ct-10670 182 26 to to ADP ct-10670 182 27 the the DET ct-10670 182 28 dish dish NOUN ct-10670 182 29 , , PUNCT ct-10670 182 30 where where SCONJ ct-10670 182 31 the the DET ct-10670 182 32 embryo embryo NOUN ct-10670 182 33 will will AUX ct-10670 182 34 thaw thaw NOUN ct-10670 182 35 and and CCONJ ct-10670 182 36 remain remain AUX ct-10670 182 37 protected protect VERB ct-10670 182 38 from from ADP ct-10670 182 39 the the DET ct-10670 182 40 light light NOUN ct-10670 182 41 for for ADP ct-10670 182 42 ~ ~ SYM ct-10670 182 43 5 5 NUM ct-10670 182 44 minutes minute NOUN ct-10670 182 45 to to PART ct-10670 182 46 allow allow VERB ct-10670 182 47 reexpansion reexpansion NOUN ct-10670 182 48 before before ADP ct-10670 182 49 loading load VERB ct-10670 182 50 into into ADP ct-10670 182 51 a a DET ct-10670 182 52 0.25 0.25 NUM ct-10670 182 53 - - PUNCT ct-10670 182 54 ml ml NOUN ct-10670 182 55 embryo embryo NOUN ct-10670 182 56 transfer transfer NOUN ct-10670 182 57 straw straw NOUN ct-10670 182 58 . . PUNCT ct-10670 183 1 then then ADV ct-10670 183 2 , , PUNCT ct-10670 183 3 the the DET ct-10670 183 4 embryo embryo NOUN ct-10670 183 5 can can AUX ct-10670 183 6 be be AUX ct-10670 183 7 loaded load VERB ct-10670 183 8 into into ADP ct-10670 183 9 an an DET ct-10670 183 10 embryo embryo NOUN ct-10670 183 11 transfer transfer NOUN ct-10670 183 12 gun gun NOUN ct-10670 183 13 . . PUNCT ct-10670 184 1 summary summary NOUN ct-10670 184 2 and and CCONJ ct-10670 184 3 future future ADJ ct-10670 184 4 plans plan VERB ct-10670 184 5 the the DET ct-10670 184 6 modern modern ADJ ct-10670 184 7 equine equine NOUN ct-10670 184 8 icsi icsi ADJ ct-10670 184 9 laboratory laboratory NOUN ct-10670 184 10 has have AUX ct-10670 184 11 become become VERB ct-10670 184 12 essential essential ADJ ct-10670 184 13 for for ADP ct-10670 184 14 complete complete ADJ ct-10670 184 15 reproductive reproductive ADJ ct-10670 184 16 management management NOUN ct-10670 184 17 of of ADP ct-10670 184 18 horses horse NOUN ct-10670 184 19 in in ADP ct-10670 184 20 the the DET ct-10670 184 21 us us PROPN ct-10670 184 22 and and CCONJ ct-10670 184 23 around around ADP ct-10670 184 24 the the DET ct-10670 184 25 world world NOUN ct-10670 184 26 . . PUNCT ct-10670 185 1 oocytes oocyte NOUN ct-10670 185 2 can can AUX ct-10670 185 3 be be AUX ct-10670 185 4 readily readily ADV ct-10670 185 5 express express ADV ct-10670 185 6 - - PUNCT ct-10670 185 7 shipped ship VERB ct-10670 185 8 to to ADP ct-10670 185 9 any any DET ct-10670 185 10 icsi icsi ADJ ct-10670 185 11 laboratory laboratory NOUN ct-10670 185 12 using use VERB ct-10670 185 13 a a DET ct-10670 185 14 variety variety NOUN ct-10670 185 15 of of ADP ct-10670 185 16 shipping shipping NOUN ct-10670 185 17 containers container NOUN ct-10670 185 18 and and CCONJ ct-10670 185 19 media medium NOUN ct-10670 185 20 . . PUNCT ct-10670 186 1 the the DET ct-10670 186 2 success success NOUN ct-10670 186 3 of of ADP ct-10670 186 4 icsi icsi NOUN ct-10670 186 5 depends depend VERB ct-10670 186 6 on on ADP ct-10670 186 7 a a DET ct-10670 186 8 multitude multitude NOUN ct-10670 186 9 of of ADP ct-10670 186 10 factors factor NOUN ct-10670 186 11 discussed discuss VERB ct-10670 186 12 , , PUNCT ct-10670 186 13 including include VERB ct-10670 186 14 mare mare NOUN ct-10670 186 15 and and CCONJ ct-10670 186 16 stallion stallion NOUN ct-10670 186 17 effects effect NOUN ct-10670 186 18 , , PUNCT ct-10670 186 19 media medium NOUN ct-10670 186 20 , , PUNCT ct-10670 186 21 culture culture NOUN ct-10670 186 22 conditions condition NOUN ct-10670 186 23 , , PUNCT ct-10670 186 24 environmental environmental ADJ ct-10670 186 25 conditions condition NOUN ct-10670 186 26 , , PUNCT ct-10670 186 27 technical technical ADJ ct-10670 186 28 experience experience NOUN ct-10670 186 29 , , PUNCT ct-10670 186 30 knowledge knowledge NOUN ct-10670 186 31 , , PUNCT ct-10670 186 32 and and CCONJ ct-10670 186 33 hygiene hygiene NOUN ct-10670 186 34 . . PUNCT ct-10670 187 1 the the DET ct-10670 187 2 time time NOUN ct-10670 187 3 - - PUNCT ct-10670 187 4 lapse lapse NOUN ct-10670 187 5 embryo embryo NOUN ct-10670 187 6 imaging imaging NOUN ct-10670 187 7 system system NOUN ct-10670 187 8 used use VERB ct-10670 187 9 in in ADP ct-10670 187 10 our our PRON ct-10670 187 11 laboratory laboratory NOUN ct-10670 187 12 allows allow VERB ct-10670 187 13 us we PRON ct-10670 187 14 to to PART ct-10670 187 15 optimize optimize VERB ct-10670 187 16 our our PRON ct-10670 187 17 commercial commercial ADJ ct-10670 187 18 and and CCONJ ct-10670 187 19 research research NOUN ct-10670 187 20 programs program NOUN ct-10670 187 21 , , PUNCT ct-10670 187 22 and and CCONJ ct-10670 187 23 it it PRON ct-10670 187 24 also also ADV ct-10670 187 25 delivers deliver VERB ct-10670 187 26 an an DET ct-10670 187 27 abundance abundance NOUN ct-10670 187 28 of of ADP ct-10670 187 29 embryo embryo NOUN ct-10670 187 30 development development NOUN ct-10670 187 31 data datum NOUN ct-10670 187 32 for for ADP ct-10670 187 33 individual individual ADJ ct-10670 187 34 embryo embryo NOUN ct-10670 187 35 analysis analysis NOUN ct-10670 187 36 . . PUNCT ct-10670 188 1 this this PRON ct-10670 188 2 allows allow VERB ct-10670 188 3 us we PRON ct-10670 188 4 to to PART ct-10670 188 5 track track VERB ct-10670 188 6 each each DET ct-10670 188 7 embryo embryo NOUN ct-10670 188 8 ’s ’s PART ct-10670 188 9 cell cell NOUN ct-10670 188 10 division division NOUN ct-10670 188 11 timing timing NOUN ct-10670 188 12 that that PRON ct-10670 188 13 provides provide VERB ct-10670 188 14 detailed detailed ADJ ct-10670 188 15 data datum NOUN ct-10670 188 16 for for ADP ct-10670 188 17 each each DET ct-10670 188 18 embryo embryo NOUN ct-10670 188 19 ’s ’s PART ct-10670 188 20 success success NOUN ct-10670 188 21 from from ADP ct-10670 188 22 fertilization fertilization NOUN ct-10670 188 23 to to ADP ct-10670 188 24 establishment establishment NOUN ct-10670 188 25 of of ADP ct-10670 188 26 pregnancy pregnancy NOUN ct-10670 188 27 . . PUNCT ct-10670 189 1 although although SCONJ ct-10670 189 2 there there PRON ct-10670 189 3 is be VERB ct-10670 189 4 no no DET ct-10670 189 5 universally universally ADV ct-10670 189 6 - - PUNCT ct-10670 189 7 agreed agree VERB ct-10670 189 8 upon upon SCONJ ct-10670 189 9 grading grade VERB ct-10670 189 10 system system NOUN ct-10670 189 11 for for ADP ct-10670 189 12 equine equine NOUN ct-10670 189 13 oocytes oocyte NOUN ct-10670 189 14 or or CCONJ ct-10670 189 15 in in ADP ct-10670 189 16 vitro vitro X ct-10670 189 17 produced produce VERB ct-10670 189 18 embryos embryo NOUN ct-10670 189 19 , , PUNCT ct-10670 189 20 recent recent ADJ ct-10670 189 21 research research NOUN ct-10670 189 22 from from ADP ct-10670 189 23 our our PRON ct-10670 189 24 laboratory laboratory NOUN ct-10670 189 25 indicated indicate VERB ct-10670 189 26 that that SCONJ ct-10670 189 27 video video NOUN ct-10670 189 28 morphokinetics morphokinetic NOUN ct-10670 189 29 may may AUX ct-10670 189 30 provide provide VERB ct-10670 189 31 a a DET ct-10670 189 32 novel novel ADJ ct-10670 189 33 method method NOUN ct-10670 189 34 to to PART ct-10670 189 35 dynamically dynamically VERB ct-10670 189 36 grade grade VERB ct-10670 189 37 equine equine NOUN ct-10670 189 38 embryos embryo NOUN ct-10670 189 39 that that PRON ct-10670 189 40 correlates correlate VERB ct-10670 189 41 well well ADV ct-10670 189 42 with with ADP ct-10670 189 43 pregnancy pregnancy NOUN ct-10670 189 44 rates rate NOUN ct-10670 189 45 after after ADP ct-10670 189 46 embryo embryo NOUN ct-10670 189 47 transfer.13,28 transfer.13,28 NOUN ct-10670 189 48 our our PRON ct-10670 189 49 main main ADJ ct-10670 189 50 field field NOUN ct-10670 189 51 of of ADP ct-10670 189 52 interest interest NOUN ct-10670 189 53 for for ADP ct-10670 189 54 future future ADJ ct-10670 189 55 research research NOUN ct-10670 189 56 is be AUX ct-10670 189 57 the the DET ct-10670 189 58 series series NOUN ct-10670 189 59 of of ADP ct-10670 189 60 complex complex ADJ ct-10670 189 61 events event NOUN ct-10670 189 62 taking take VERB ct-10670 189 63 place place NOUN ct-10670 189 64 around around ADP ct-10670 189 65 the the DET ct-10670 189 66 first first ADJ ct-10670 189 67 cleavage cleavage NOUN ct-10670 189 68 division,13 division,13 NOUN ct-10670 189 69 such such ADJ ct-10670 189 70 as as ADP ct-10670 189 71 cytoplasmic cytoplasmic ADJ ct-10670 189 72 extrusion extrusion NOUN ct-10670 189 73 and and CCONJ ct-10670 189 74 pronuclei pronuclei NOUN ct-10670 189 75 formation formation NOUN ct-10670 189 76 , , PUNCT ct-10670 189 77 which which PRON ct-10670 189 78 seem seem VERB ct-10670 189 79 to to PART ct-10670 189 80 be be AUX ct-10670 189 81 good good ADJ ct-10670 189 82 predictors predictor NOUN ct-10670 189 83 of of ADP ct-10670 189 84 the the DET ct-10670 189 85 time time NOUN ct-10670 189 86 of of ADP ct-10670 189 87 cleavage cleavage NOUN ct-10670 189 88 , , PUNCT ct-10670 189 89 aneuploidy aneuploidy VERB ct-10670 189 90 , , PUNCT ct-10670 189 91 and and CCONJ ct-10670 189 92 overall overall ADJ ct-10670 189 93 developmental developmental ADJ ct-10670 189 94 potential potential NOUN ct-10670 189 95 . . PUNCT ct-10670 190 1 in in ADP ct-10670 190 2 addition addition NOUN ct-10670 190 3 to to ADP ct-10670 190 4 time time NOUN ct-10670 190 5 - - PUNCT ct-10670 190 6 lapse lapse NOUN ct-10670 190 7 characterization characterization NOUN ct-10670 190 8 of of ADP ct-10670 190 9 early early ADJ ct-10670 190 10 embryonic embryonic ADJ ct-10670 190 11 events event NOUN ct-10670 190 12 , , PUNCT ct-10670 190 13 we we PRON ct-10670 190 14 and and CCONJ ct-10670 190 15 others other NOUN ct-10670 190 16 utilize utilize VERB ct-10670 190 17 immunofluorescence immunofluorescence NOUN ct-10670 190 18 and and CCONJ ct-10670 190 19 transcriptomics13,25,27,36,37 transcriptomics13,25,27,36,37 NOUN ct-10670 190 20 to to PART ct-10670 190 21 broaden broaden VERB ct-10670 190 22 our our PRON ct-10670 190 23 insight insight NOUN ct-10670 190 24 into into ADP ct-10670 190 25 this this DET ct-10670 190 26 period period NOUN ct-10670 190 27 of of ADP ct-10670 190 28 equine equine NOUN ct-10670 190 29 development development NOUN ct-10670 190 30 . . PUNCT ct-10670 191 1 conflict conflict NOUN ct-10670 191 2 of of ADP ct-10670 191 3 interest interest NOUN ct-10670 191 4 none none NOUN ct-10670 191 5 to to PART ct-10670 191 6 report report VERB ct-10670 191 7 references reference NOUN ct-10670 191 8 1 1 NUM ct-10670 191 9 . . PUNCT ct-10670 192 1 ombelet ombelet PROPN ct-10670 192 2 w w PROPN ct-10670 192 3 , , PUNCT ct-10670 192 4 van van PROPN ct-10670 192 5 robays robay VERB ct-10670 192 6 j j NOUN ct-10670 192 7 : : PUNCT ct-10670 192 8 artificial artificial ADJ ct-10670 192 9 insemination insemination NOUN ct-10670 192 10 history history NOUN ct-10670 192 11 : : PUNCT ct-10670 192 12 hurdles hurdle NOUN ct-10670 192 13 and and CCONJ ct-10670 192 14 milestones milestone NOUN ct-10670 192 15 . . PUNCT ct-10670 193 1 facts fact NOUN ct-10670 193 2 views view NOUN ct-10670 193 3 vis vis X ct-10670 193 4 obgyn obgyn PROPN ct-10670 193 5 2015;7:137 2015;7:137 NOUN ct-10670 193 6 - - SYM ct-10670 193 7 143 143 NUM ct-10670 193 8 . . NOUN ct-10670 194 1 2 2 X ct-10670 194 2 . . X ct-10670 194 3 hochi hochi PROPN ct-10670 194 4 s s PROPN ct-10670 194 5 , , PUNCT ct-10670 194 6 maruyama maruyama PROPN ct-10670 194 7 k k PROPN ct-10670 194 8 , , PUNCT ct-10670 194 9 oguri oguri PROPN ct-10670 194 10 n n CCONJ ct-10670 194 11 : : PUNCT ct-10670 194 12 direct direct ADJ ct-10670 194 13 transfer transfer NOUN ct-10670 194 14 of of ADP ct-10670 194 15 equine equine NOUN ct-10670 194 16 blastocysts blastocyst NOUN ct-10670 194 17 frozen frozen ADV ct-10670 194 18 - - PUNCT ct-10670 194 19 thawed thaw VERB ct-10670 194 20 in in ADP ct-10670 194 21 the the DET ct-10670 194 22 presence presence NOUN ct-10670 194 23 of of ADP ct-10670 194 24 ethylene ethylene NOUN ct-10670 194 25 glycol glycol NOUN ct-10670 194 26 and and CCONJ ct-10670 194 27 sucrose sucrose NOUN ct-10670 194 28 . . PUNCT ct-10670 195 1 theriogenology theriogenology NOUN ct-10670 195 2 1996;46:1217 1996;46:1217 NUM ct-10670 195 3 - - SYM ct-10670 195 4 1224 1224 NUM ct-10670 195 5 . . PUNCT ct-10670 196 1 doi doi NOUN ct-10670 196 2 : : PUNCT ct-10670 196 3 10.1016/ 10.1016/ NUM ct-10670 196 4 s0093 s0093 PROPN ct-10670 196 5 - - PUNCT ct-10670 196 6 691x(96)00292 691x(96)00292 PROPN ct-10670 196 7 - - PUNCT ct-10670 196 8 0 0 NUM ct-10670 196 9 3 3 NUM ct-10670 196 10 . . PUNCT ct-10670 197 1 hochi hochi PROPN ct-10670 197 2 s s PROPN ct-10670 197 3 , , PUNCT ct-10670 197 4 kozawa kozawa PROPN ct-10670 197 5 m m PROPN ct-10670 197 6 , , PUNCT ct-10670 197 7 fujimoto fujimoto PROPN ct-10670 197 8 t t PROPN ct-10670 197 9 , , PUNCT ct-10670 197 10 et et PROPN ct-10670 197 11 al al PROPN ct-10670 197 12 : : PUNCT ct-10670 197 13 in in ADP ct-10670 197 14 vitro vitro X ct-10670 197 15 maturation maturation NOUN ct-10670 197 16 and and CCONJ ct-10670 197 17 transmission transmission NOUN ct-10670 197 18 electron electron NOUN ct-10670 197 19 microscopic microscopic ADJ ct-10670 197 20 observation observation NOUN ct-10670 197 21 of of ADP ct-10670 197 22 horse horse NOUN ct-10670 197 23 oocytes oocyte NOUN ct-10670 197 24 after after ADP ct-10670 197 25 vitrification vitrification NOUN ct-10670 197 26 . . PUNCT ct-10670 198 1 cryobiology cryobiology NOUN ct-10670 198 2 1996;33:300 1996;33:300 NUM ct-10670 198 3 - - NUM ct-10670 198 4 310 310 NUM ct-10670 198 5 . . PUNCT ct-10670 199 1 doi doi NOUN ct-10670 199 2 : : PUNCT ct-10670 199 3 10.1006/ 10.1006/ NUM ct-10670 199 4 cryo.1996.0030 cryo.1996.0030 NOUN ct-10670 199 5 4 4 NUM ct-10670 199 6 . . PUNCT ct-10670 200 1 yuan yuan PROPN ct-10670 200 2 z z PROPN ct-10670 200 3 , , PUNCT ct-10670 200 4 yuan yuan PROPN ct-10670 200 5 m m PROPN ct-10670 200 6 , , PUNCT ct-10670 200 7 song song NOUN ct-10670 200 8 x x NOUN ct-10670 200 9 , , PUNCT ct-10670 200 10 et et PROPN ct-10670 200 11 al al PROPN ct-10670 200 12 : : PUNCT ct-10670 200 13 development development NOUN ct-10670 200 14 of of ADP ct-10670 200 15 an an DET ct-10670 200 16 artificial artificial ADJ ct-10670 200 17 intelligence intelligence NOUN ct-10670 200 18 based base VERB ct-10670 200 19 model model NOUN ct-10670 200 20 for for ADP ct-10670 200 21 predicting predict VERB ct-10670 200 22 the the DET ct-10670 200 23 euploidy euploidy NOUN ct-10670 200 24 of of ADP ct-10670 200 25 blastocysts blastocyst NOUN ct-10670 200 26 in in ADP ct-10670 200 27 pgt pgt PROPN ct-10670 200 28 - - PUNCT ct-10670 200 29 a a DET ct-10670 200 30 treatments treatment NOUN ct-10670 200 31 . . PUNCT ct-10670 201 1 sci sci PROPN ct-10670 201 2 rep rep PROPN ct-10670 201 3 2023;13:2322 2023;13:2322 PROPN ct-10670 201 4 . . PUNCT ct-10670 201 5 doi doi PROPN ct-10670 201 6 : : PUNCT ct-10670 201 7 10.1038/ 10.1038/ NUM ct-10670 201 8 s41598 s41598 PROPN ct-10670 201 9 - - PUNCT ct-10670 201 10 023 023 NUM ct-10670 201 11 - - PUNCT ct-10670 201 12 29319 29319 NUM ct-10670 201 13 - - PUNCT ct-10670 201 14 z z NOUN ct-10670 201 15 5 5 NUM ct-10670 201 16 . . PUNCT ct-10670 201 17 palermo palermo PROPN ct-10670 201 18 g g PROPN ct-10670 201 19 , , PUNCT ct-10670 201 20 joris joris PROPN ct-10670 201 21 h h PROPN ct-10670 201 22 , , PUNCT ct-10670 201 23 devroey devroey VERB ct-10670 201 24 p p X ct-10670 201 25 , , PUNCT ct-10670 201 26 et et PROPN ct-10670 201 27 al al PROPN ct-10670 201 28 : : PUNCT ct-10670 201 29 pregnancies pregnancy NOUN ct-10670 201 30 after after ADP ct-10670 201 31 intracytoplasmic intracytoplasmic ADJ ct-10670 201 32 injection injection NOUN ct-10670 201 33 of of ADP ct-10670 201 34 single single ADJ ct-10670 201 35 spermatozoon spermatozoon NOUN ct-10670 201 36 into into ADP ct-10670 201 37 an an DET ct-10670 201 38 oocyte oocyte NOUN ct-10670 201 39 . . PUNCT ct-10670 202 1 lancet lancet PROPN ct-10670 202 2 1992;340:17 1992;340:17 NUM ct-10670 202 3 - - SYM ct-10670 202 4 18 18 NUM ct-10670 202 5 . . PUNCT ct-10670 203 1 doi doi NOUN ct-10670 203 2 : : PUNCT ct-10670 203 3 10.1016/0140 10.1016/0140 NUM ct-10670 203 4 - - SYM ct-10670 203 5 6736(92)92425 6736(92)92425 NUM ct-10670 203 6 - - PUNCT ct-10670 203 7 f f PROPN ct-10670 203 8 6 6 NUM ct-10670 203 9 . . PUNCT ct-10670 204 1 pereira pereira PROPN ct-10670 204 2 n n PROPN ct-10670 204 3 , , PUNCT ct-10670 204 4 cozzubbo cozzubbo PROPN ct-10670 204 5 t t PROPN ct-10670 204 6 , , PUNCT ct-10670 204 7 cheung cheung PROPN ct-10670 204 8 s s PROPN ct-10670 204 9 , , PUNCT ct-10670 204 10 et et PROPN ct-10670 204 11 al al PROPN ct-10670 204 12 : : PUNCT ct-10670 204 13 lessons lesson NOUN ct-10670 204 14 learned learn VERB ct-10670 204 15 in in ADP ct-10670 204 16 andrology andrology NOUN ct-10670 204 17 : : PUNCT ct-10670 204 18 from from ADP ct-10670 204 19 intracytoplasmic intracytoplasmic ADJ ct-10670 204 20 sperm sperm NOUN ct-10670 204 21 injection injection NOUN ct-10670 204 22 and and CCONJ ct-10670 204 23 beyond beyond ADP ct-10670 204 24 . . PUNCT ct-10670 205 1 andrology andrology NOUN ct-10670 205 2 2016;4:757 2016;4:757 PROPN ct-10670 205 3 - - SYM ct-10670 205 4 760 760 NUM ct-10670 205 5 . . PUNCT ct-10670 205 6 doi doi NOUN ct-10670 205 7 : : PUNCT ct-10670 205 8 10.1111 10.1111 NUM ct-10670 205 9 / / SYM ct-10670 205 10 andr.12225 andr.12225 PROPN ct-10670 205 11 7 7 NUM ct-10670 205 12 . . PUNCT ct-10670 205 13 morris morris PROPN ct-10670 205 14 lha lha PROPN ct-10670 205 15 . . PUNCT ct-10670 206 1 the the DET ct-10670 206 2 development development NOUN ct-10670 206 3 of of ADP ct-10670 206 4 in in ADP ct-10670 206 5 vitro vitro X ct-10670 206 6 embryo embryo NOUN ct-10670 206 7 production production NOUN ct-10670 206 8 in in ADP ct-10670 206 9 the the DET ct-10670 206 10 horse horse NOUN ct-10670 206 11 . . PUNCT ct-10670 207 1 equine equine NOUN ct-10670 207 2 vet vet NOUN ct-10670 207 3 j j PROPN ct-10670 207 4 2018;50:712 2018;50:712 NOUN ct-10670 207 5 - - PUNCT ct-10670 207 6 720 720 NUM ct-10670 207 7 . . PUNCT ct-10670 208 1 doi doi NOUN ct-10670 208 2 : : PUNCT ct-10670 208 3 10.1111 10.1111 NUM ct-10670 208 4 / / SYM ct-10670 208 5 evj.12839 evj.12839 NUM ct-10670 208 6 8 8 NUM ct-10670 208 7 . . PUNCT ct-10670 209 1 carnevale carnevale NOUN ct-10670 209 2 em em PRON ct-10670 209 3 , , PUNCT ct-10670 209 4 sessions session NOUN ct-10670 209 5 dr dr PROPN ct-10670 209 6 : : PUNCT ct-10670 209 7 in in ADP ct-10670 209 8 vitro vitro X ct-10670 209 9 production production NOUN ct-10670 209 10 of of ADP ct-10670 209 11 equine equine NOUN ct-10670 209 12 embryos embryo NOUN ct-10670 209 13 . . PUNCT ct-10670 210 1 j j PROPN ct-10670 210 2 equine equine PROPN ct-10670 210 3 vet vet NOUN ct-10670 210 4 sci sci PROPN ct-10670 210 5 2012;32:367 2012;32:367 PROPN ct-10670 210 6 - - SYM ct-10670 210 7 371 371 NUM ct-10670 210 8 . . PUNCT ct-10670 211 1 doi doi NOUN ct-10670 211 2 : : PUNCT ct-10670 211 3 10.1016 10.1016 NUM ct-10670 211 4 / / SYM ct-10670 211 5 j.jevs j.jevs NOUN ct-10670 211 6 . . PUNCT ct-10670 212 1 2012.05.054 2012.05.054 NUM ct-10670 212 2 9 9 NUM ct-10670 212 3 . . PUNCT ct-10670 213 1 choi choi PROPN ct-10670 213 2 yh yh PROPN ct-10670 213 3 , , PUNCT ct-10670 213 4 roasa roasa NOUN ct-10670 213 5 lm lm PROPN ct-10670 213 6 , , PUNCT ct-10670 213 7 love love PROPN ct-10670 213 8 cc cc PROPN ct-10670 213 9 , , PUNCT ct-10670 213 10 et et PROPN ct-10670 213 11 al al PROPN ct-10670 213 12 : : PUNCT ct-10670 213 13 blastocyst blastocyst NOUN ct-10670 213 14 formation formation NOUN ct-10670 213 15 rates rate NOUN ct-10670 213 16 in in ADP ct-10670 213 17 vivo vivo NOUN ct-10670 213 18 and and CCONJ ct-10670 213 19 in in ADP ct-10670 213 20 vitro vitro X ct-10670 213 21 of of ADP ct-10670 213 22 in in ADP ct-10670 213 23 vitro vitro X ct-10670 213 24 - - PUNCT ct-10670 213 25 matured mature VERB ct-10670 213 26 equine equine NOUN ct-10670 213 27 oocytes oocyte NOUN ct-10670 213 28 fertilized fertilize VERB ct-10670 213 29 by by ADP ct-10670 213 30 intracytoplasmic intracytoplasmic ADJ ct-10670 213 31 sperm sperm NOUN ct-10670 213 32 injection injection NOUN ct-10670 213 33 . . PUNCT ct-10670 214 1 biol biol PROPN ct-10670 214 2 reprod reprod PROPN ct-10670 214 3 2004;70:12311238 2004;70:12311238 NUM ct-10670 214 4 . . PUNCT ct-10670 214 5 doi doi PROPN ct-10670 214 6 : : PUNCT ct-10670 214 7 10.1095 10.1095 NUM ct-10670 214 8 / / SYM ct-10670 214 9 biolreprod.103.023903 biolreprod.103.023903 NOUN ct-10670 214 10 10 10 NUM ct-10670 214 11 . . PUNCT ct-10670 215 1 hinrichs hinrichs PROPN ct-10670 215 2 k k PROPN ct-10670 215 3 , , PUNCT ct-10670 215 4 choi choi PROPN ct-10670 215 5 yh yh PROPN ct-10670 215 6 , , PUNCT ct-10670 215 7 walckenaer walckenaer NOUN ct-10670 215 8 be be AUX ct-10670 215 9 , , PUNCT ct-10670 215 10 et et PROPN ct-10670 215 11 al al PROPN ct-10670 215 12 : : PUNCT ct-10670 215 13 in in ADP ct-10670 215 14 vitro vitro X ct-10670 215 15 - - PUNCT ct-10670 215 16 produced produce VERB ct-10670 215 17 equine equine NOUN ct-10670 215 18 embryos embryo NOUN ct-10670 215 19 : : PUNCT ct-10670 215 20 production production NOUN ct-10670 215 21 of of ADP ct-10670 215 22 foals foal NOUN ct-10670 215 23 after after ADP ct-10670 215 24 transfer transfer NOUN ct-10670 215 25 , , PUNCT ct-10670 215 26 assessment assessment NOUN ct-10670 215 27 by by ADP ct-10670 215 28 differential differential ADJ ct-10670 215 29 staining staining NOUN ct-10670 215 30 and and CCONJ ct-10670 215 31 effect effect NOUN ct-10670 215 32 of of ADP ct-10670 215 33 medium medium ADJ ct-10670 215 34 calcium calcium NOUN ct-10670 215 35 concentrations concentration NOUN ct-10670 215 36 during during ADP ct-10670 215 37 culture culture NOUN ct-10670 215 38 . . PUNCT ct-10670 216 1 theriogenology theriogenology NOUN ct-10670 216 2 2007;68:521 2007;68:521 NOUN ct-10670 216 3 - - SYM ct-10670 216 4 529 529 NUM ct-10670 216 5 . . PUNCT ct-10670 217 1 doi doi NOUN ct-10670 217 2 : : PUNCT ct-10670 217 3 10.1016/ 10.1016/ NUM ct-10670 217 4 j.theriogenology.2007.04.046 j.theriogenology.2007.04.046 ADV ct-10670 217 5 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PRON ct-10670 217 6 https://doi.org/10.1016/s0093-691x(96)00292-0 https://doi.org/10.1016/s0093-691x(96)00292-0 ADJ ct-10670 217 7 https://doi.org/10.1016/s0093-691x(96)00292-0 https://doi.org/10.1016/s0093-691x(96)00292-0 ADJ ct-10670 217 8 https://doi.org/10.1006/cryo.1996.0030 https://doi.org/10.1006/cryo.1996.0030 PROPN ct-10670 218 1 https://doi.org/10.1006/cryo.1996.0030 https://doi.org/10.1006/cryo.1996.0030 PROPN ct-10670 219 1 https://doi.org/10.1038/s41598-023-29319-z https://doi.org/10.1038/s41598-023-29319-z PROPN ct-10670 219 2 https://doi.org/10.1038/s41598-023-29319-z https://doi.org/10.1038/s41598-023-29319-z PROPN ct-10670 219 3 https://doi.org/10.1016/0140-6736(92)92425-f https://doi.org/10.1016/0140-6736(92)92425-f PROPN ct-10670 219 4 https://doi.org/10.1111/andr.12225 https://doi.org/10.1111/andr.12225 PROPN ct-10670 219 5 https://doi.org/10.1111/evj.12839 https://doi.org/10.1111/evj.12839 PROPN ct-10670 219 6 https://doi.org/10.1016/j.jevs.2012.05.054 https://doi.org/10.1016/j.jevs.2012.05.054 VERB ct-10670 219 7 https://doi.org/10.1016/j.jevs.2012.05.054 https://doi.org/10.1016/j.jevs.2012.05.054 PROPN ct-10670 219 8 https://doi.org/10.1095/biolreprod.103.023903 https://doi.org/10.1095/biolreprod.103.023903 PROPN ct-10670 219 9 https://doi.org/10.1016/j.theriogenology.2007.04.046 https://doi.org/10.1016/j.theriogenology.2007.04.046 NOUN ct-10670 219 10 https://doi.org/10.1016/j.theriogenology.2007.04.046 https://doi.org/10.1016/j.theriogenology.2007.04.046 NOUN ct-10670 219 11 citation citation NOUN ct-10670 219 12 : : PUNCT ct-10670 219 13 clinical clinical ADJ ct-10670 219 14 theriogenology theriogenology NOUN ct-10670 219 15 2024 2024 NUM ct-10670 219 16 , , PUNCT ct-10670 219 17 16 16 NUM ct-10670 219 18 , , PUNCT ct-10670 219 19 10670 10670 NUM ct-10670 219 20 , , PUNCT ct-10670 219 21 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PROPN ct-10670 219 22 9 9 NUM ct-10670 219 23 11 11 NUM ct-10670 219 24 . . PUNCT ct-10670 220 1 foss foss PROPN ct-10670 220 2 r r PROPN ct-10670 220 3 , , PUNCT ct-10670 220 4 ortis ortis ADJ ct-10670 220 5 h h NOUN ct-10670 220 6 , , PUNCT ct-10670 220 7 hinrichs hinrichs ADJ ct-10670 220 8 k k NOUN ct-10670 220 9 : : PUNCT ct-10670 220 10 effect effect NOUN ct-10670 220 11 of of ADP ct-10670 220 12 potential potential ADJ ct-10670 220 13 oocyte oocyte ADJ ct-10670 220 14 transport transport NOUN ct-10670 220 15 protocols protocol NOUN ct-10670 220 16 on on ADP ct-10670 220 17 blastocyst blastocyst NOUN ct-10670 220 18 rates rate NOUN ct-10670 220 19 after after ADP ct-10670 220 20 intracytoplasmic intracytoplasmic ADJ ct-10670 220 21 sperm sperm NOUN ct-10670 220 22 injection injection NOUN ct-10670 220 23 in in ADP ct-10670 220 24 the the DET ct-10670 220 25 horse horse NOUN ct-10670 220 26 . . PUNCT ct-10670 221 1 equine equine NOUN ct-10670 221 2 vet vet PROPN ct-10670 221 3 j j PROPN ct-10670 221 4 2013;45:39 2013;45:39 NUM ct-10670 221 5 - - SYM ct-10670 221 6 43 43 NUM ct-10670 221 7 . . PUNCT ct-10670 222 1 doi doi NOUN ct-10670 222 2 : : PUNCT ct-10670 222 3 10.1111/ 10.1111/ NUM ct-10670 222 4 evj.12159 evj.12159 PROPN ct-10670 222 5 12 12 NUM ct-10670 222 6 . . PUNCT ct-10670 223 1 lazzari lazzari PROPN ct-10670 223 2 g g PROPN ct-10670 223 3 , , PUNCT ct-10670 223 4 colleoni colleoni PROPN ct-10670 223 5 s s PROPN ct-10670 223 6 , , PUNCT ct-10670 223 7 crotti crotti PROPN ct-10670 223 8 g g PROPN ct-10670 223 9 , , PUNCT ct-10670 223 10 et et PROPN ct-10670 223 11 al al PROPN ct-10670 223 12 : : PUNCT ct-10670 223 13 laboratory laboratory NOUN ct-10670 223 14 production production NOUN ct-10670 223 15 of of ADP ct-10670 223 16 equine equine NOUN ct-10670 223 17 embryos embryo NOUN ct-10670 223 18 . . PUNCT ct-10670 224 1 j j PROPN ct-10670 224 2 equine equine PROPN ct-10670 224 3 vet vet NOUN ct-10670 224 4 sci sci PROPN ct-10670 224 5 2020;89:103097 2020;89:103097 PROPN ct-10670 224 6 . . PUNCT ct-10670 225 1 doi doi NOUN ct-10670 225 2 : : PUNCT ct-10670 225 3 10.1016/ 10.1016/ NUM ct-10670 225 4 j.jevs.2020.103097 j.jevs.2020.103097 NOUN ct-10670 225 5 13 13 NUM ct-10670 225 6 . . PUNCT ct-10670 226 1 meyers meyers PROPN ct-10670 226 2 s s PROPN ct-10670 226 3 , , PUNCT ct-10670 226 4 burruel burruel PROPN ct-10670 226 5 v v PROPN ct-10670 226 6 , , PUNCT ct-10670 226 7 kato kato PROPN ct-10670 226 8 m m PROPN ct-10670 226 9 , , PUNCT ct-10670 226 10 et et PROPN ct-10670 226 11 al al PROPN ct-10670 226 12 : : PUNCT ct-10670 226 13 equine equine VERB ct-10670 226 14 non non ADJ ct-10670 226 15 - - ADJ ct-10670 226 16 invasive invasive ADJ ct-10670 226 17 time time NOUN ct-10670 226 18 - - PUNCT ct-10670 226 19 lapse lapse NOUN ct-10670 226 20 imaging imaging NOUN ct-10670 226 21 and and CCONJ ct-10670 226 22 blastocyst blastocyst NOUN ct-10670 226 23 development development NOUN ct-10670 226 24 . . PUNCT ct-10670 227 1 reprod reprod PROPN ct-10670 227 2 fertil fertil PROPN ct-10670 227 3 dev dev PROPN ct-10670 227 4 2019;31:1874 2019;31:1874 PROPN ct-10670 227 5 - - SYM ct-10670 227 6 1884 1884 NUM ct-10670 227 7 . . PUNCT ct-10670 228 1 doi doi NOUN ct-10670 228 2 : : PUNCT ct-10670 228 3 10.1071 10.1071 NUM ct-10670 228 4 / / SYM ct-10670 228 5 rd19260 rd19260 NOUN ct-10670 228 6 14 14 NUM ct-10670 228 7 . . PUNCT ct-10670 229 1 felix felix PROPN ct-10670 229 2 mr mr PROPN ct-10670 229 3 , , PUNCT ct-10670 229 4 turner turner PROPN ct-10670 229 5 rm rm PROPN ct-10670 229 6 , , PUNCT ct-10670 229 7 dobbie dobbie PROPN ct-10670 229 8 t t PROPN ct-10670 229 9 , , PUNCT ct-10670 229 10 et et PROPN ct-10670 229 11 al al PROPN ct-10670 229 12 : : PUNCT ct-10670 229 13 successful successful ADJ ct-10670 229 14 in in ADP ct-10670 229 15 vitro vitro X ct-10670 229 16 fertilization fertilization NOUN ct-10670 229 17 in in ADP ct-10670 229 18 the the DET ct-10670 229 19 horse horse NOUN ct-10670 229 20 : : PUNCT ct-10670 229 21 production production NOUN ct-10670 229 22 of of ADP ct-10670 229 23 blastocysts blastocyst NOUN ct-10670 229 24 and and CCONJ ct-10670 229 25 birth birth NOUN ct-10670 229 26 of of ADP ct-10670 229 27 foals foal NOUN ct-10670 229 28 after after ADP ct-10670 229 29 prolonged prolong VERB ct-10670 229 30 sperm sperm NOUN ct-10670 229 31 incubation incubation NOUN ct-10670 229 32 for for ADP ct-10670 229 33 capacitation†. capacitation†. NUM ct-10670 229 34 biol biol PROPN ct-10670 229 35 reprod reprod X ct-10670 229 36 2022;107:1551 2022;107:1551 NUM ct-10670 229 37 - - SYM ct-10670 229 38 1564 1564 NUM ct-10670 229 39 . . PUNCT ct-10670 230 1 doi doi NOUN ct-10670 230 2 : : PUNCT ct-10670 230 3 10.1093 10.1093 NUM ct-10670 230 4 / / SYM ct-10670 230 5 biolre biolre NOUN ct-10670 230 6 / / SYM ct-10670 230 7 ioac172 ioac172 NOUN ct-10670 230 8 15 15 NUM ct-10670 230 9 . . PUNCT ct-10670 231 1 metcalf metcalf PROPN ct-10670 231 2 es es PROPN ct-10670 231 3 , , PUNCT ct-10670 231 4 masterson masterson PROPN ct-10670 231 5 kr kr PROPN ct-10670 231 6 , , PUNCT ct-10670 231 7 battaglia battaglia PROPN ct-10670 232 1 d d PROPN ct-10670 232 2 , , PUNCT ct-10670 232 3 et et PROPN ct-10670 232 4 al al PROPN ct-10670 232 5 : : PUNCT ct-10670 232 6 conditions condition NOUN ct-10670 232 7 to to PART ct-10670 232 8 optimise optimise VERB ct-10670 232 9 the the DET ct-10670 232 10 developmental developmental ADJ ct-10670 232 11 competence competence NOUN ct-10670 232 12 of of ADP ct-10670 232 13 immature immature ADJ ct-10670 232 14 equine equine NOUN ct-10670 232 15 oocytes oocyte NOUN ct-10670 232 16 . . PUNCT ct-10670 233 1 reprod reprod PROPN ct-10670 233 2 fertil fertil AUX ct-10670 233 3 dev dev VERB ct-10670 233 4 2020;32:1012 2020;32:1012 PROPN ct-10670 233 5 - - SYM ct-10670 233 6 1021 1021 NUM ct-10670 233 7 . . PUNCT ct-10670 234 1 doi doi NOUN ct-10670 234 2 : : PUNCT ct-10670 234 3 10.1071 10.1071 NUM ct-10670 234 4 / / SYM ct-10670 234 5 rd19249 rd19249 NOUN ct-10670 234 6 16 16 NUM ct-10670 234 7 . . PUNCT ct-10670 235 1 lorenzen lorenzen PROPN ct-10670 235 2 e e PROPN ct-10670 235 3 , , PUNCT ct-10670 235 4 carstensen carstensen VERB ct-10670 235 5 a a DET ct-10670 235 6 , , PUNCT ct-10670 235 7 winther winther PROPN ct-10670 235 8 m m PROPN ct-10670 235 9 , , PUNCT ct-10670 235 10 et et PROPN ct-10670 235 11 al al PROPN ct-10670 235 12 : : PUNCT ct-10670 235 13 factors factor NOUN ct-10670 235 14 affecting affect VERB ct-10670 235 15 post post ADJ ct-10670 235 16 - - ADJ ct-10670 235 17 mortem mortem ADJ ct-10670 235 18 production production NOUN ct-10670 235 19 of of ADP ct-10670 235 20 equine equine NOUN ct-10670 235 21 icsi icsi ADJ ct-10670 235 22 blastocysts blastocyst NOUN ct-10670 235 23 . . PUNCT ct-10670 236 1 j j PROPN ct-10670 236 2 equine equine PROPN ct-10670 236 3 vet vet NOUN ct-10670 236 4 sci sci PROPN ct-10670 236 5 2023;125:104660 2023;125:104660 NUM ct-10670 236 6 . . PUNCT ct-10670 237 1 doi doi NOUN ct-10670 237 2 : : PUNCT ct-10670 237 3 10.1016 10.1016 NUM ct-10670 237 4 / / SYM ct-10670 237 5 j.jevs.2023.104660 j.jevs.2023.104660 NOUN ct-10670 237 6 17 17 NUM ct-10670 237 7 . . PUNCT ct-10670 238 1 martin martin PROPN ct-10670 238 2 - - PUNCT ct-10670 238 3 pelaez pelaez PROPN ct-10670 238 4 s s PROPN ct-10670 238 5 , , PUNCT ct-10670 238 6 rabow rabow VERB ct-10670 238 7 z z PROPN ct-10670 238 8 , , PUNCT ct-10670 238 9 de de X ct-10670 238 10 la la X ct-10670 238 11 fuente fuente X ct-10670 238 12 a a X ct-10670 238 13 , , PUNCT ct-10670 238 14 et et PROPN ct-10670 238 15 al al PROPN ct-10670 238 16 : : PUNCT ct-10670 238 17 effect effect NOUN ct-10670 238 18 of of ADP ct-10670 238 19 pentobarbital pentobarbital NOUN ct-10670 238 20 as as ADP ct-10670 238 21 a a DET ct-10670 238 22 euthanasia euthanasia NOUN ct-10670 238 23 agent agent NOUN ct-10670 238 24 on on ADP ct-10670 238 25 equine equine NOUN ct-10670 238 26 in in ADP ct-10670 238 27 vitro vitro X ct-10670 238 28 embryo embryo NOUN ct-10670 238 29 production production NOUN ct-10670 238 30 . . PUNCT ct-10670 239 1 theriogenology theriogenology NOUN ct-10670 239 2 2023;205:1 2023;205:1 NUM ct-10670 239 3 - - SYM ct-10670 239 4 8 8 NUM ct-10670 239 5 . . PUNCT ct-10670 239 6 doi doi NOUN ct-10670 239 7 : : PUNCT ct-10670 239 8 10.1016/ 10.1016/ NUM ct-10670 239 9 j.theriogenology.2023.04.002 j.theriogenology.2023.04.002 PROPN ct-10670 239 10 18 18 NUM ct-10670 239 11 . . PUNCT ct-10670 240 1 iacono iacono PROPN ct-10670 240 2 e e NOUN ct-10670 240 3 , , PUNCT ct-10670 240 4 merlo merlo PROPN ct-10670 240 5 b b PROPN ct-10670 240 6 , , PUNCT ct-10670 240 7 rizzato rizzato VERB ct-10670 240 8 g g PROPN ct-10670 240 9 , , PUNCT ct-10670 240 10 et et PROPN ct-10670 240 11 al al PROPN ct-10670 240 12 : : PUNCT ct-10670 240 13 effects effect NOUN ct-10670 240 14 of of ADP ct-10670 240 15 repeated repeat VERB ct-10670 240 16 transvaginal transvaginal ADJ ct-10670 240 17 ultrasound ultrasound NOUN ct-10670 240 18 - - PUNCT ct-10670 240 19 guided guide VERB ct-10670 240 20 aspirations aspiration NOUN ct-10670 240 21 performed perform VERB ct-10670 240 22 in in ADP ct-10670 240 23 anestrous anestrous ADJ ct-10670 240 24 and and CCONJ ct-10670 240 25 cyclic cyclic ADJ ct-10670 240 26 mares mare NOUN ct-10670 240 27 on on ADP ct-10670 240 28 p4 p4 ADJ ct-10670 240 29 and and CCONJ ct-10670 240 30 e2 e2 NOUN ct-10670 240 31 plasma plasma NOUN ct-10670 240 32 levels level NOUN ct-10670 240 33 and and CCONJ ct-10670 240 34 luteal luteal ADJ ct-10670 240 35 function function NOUN ct-10670 240 36 . . PUNCT ct-10670 241 1 theriogenology theriogenology NOUN ct-10670 241 2 2014;82:225 2014;82:225 NOUN ct-10670 241 3 - - SYM ct-10670 241 4 231 231 NUM ct-10670 241 5 . . PUNCT ct-10670 242 1 doi doi NOUN ct-10670 242 2 : : PUNCT ct-10670 242 3 10.1016/ 10.1016/ NUM ct-10670 242 4 j.theriogenology.2014.03.025 j.theriogenology.2014.03.025 NOUN ct-10670 242 5 19 19 NUM ct-10670 242 6 . . PUNCT ct-10670 242 7 velez velez PROPN ct-10670 242 8 ic ic PROPN ct-10670 242 9 , , PUNCT ct-10670 242 10 arnold arnold PROPN ct-10670 242 11 c c PROPN ct-10670 242 12 , , PUNCT ct-10670 242 13 jacobson jacobson PROPN ct-10670 242 14 cc cc PROPN ct-10670 242 15 , , PUNCT ct-10670 242 16 et et PROPN ct-10670 242 17 al al PROPN ct-10670 242 18 : : PUNCT ct-10670 242 19 effects effect NOUN ct-10670 242 20 of of ADP ct-10670 242 21 repeated repeat VERB ct-10670 242 22 transvaginal transvaginal ADJ ct-10670 242 23 aspiration aspiration NOUN ct-10670 242 24 of of ADP ct-10670 242 25 immature immature ADJ ct-10670 242 26 follicles follicle NOUN ct-10670 242 27 on on ADP ct-10670 242 28 mare mare NOUN ct-10670 242 29 health health NOUN ct-10670 242 30 and and CCONJ ct-10670 242 31 ovarian ovarian ADJ ct-10670 242 32 status status NOUN ct-10670 242 33 . . PUNCT ct-10670 243 1 equine equine NOUN ct-10670 243 2 vet vet PROPN ct-10670 243 3 j j PROPN ct-10670 243 4 2012;43:78 2012;43:78 PROPN ct-10670 243 5 - - PUNCT ct-10670 243 6 83 83 NUM ct-10670 243 7 . . PUNCT ct-10670 244 1 doi doi NOUN ct-10670 244 2 : : PUNCT ct-10670 244 3 10.1111 10.1111 NUM ct-10670 244 4 / / SYM ct-10670 244 5 j.2042 j.2042 NOUN ct-10670 244 6 - - PUNCT ct-10670 244 7 3306.2012.00606.x 3306.2012.00606.x NUM ct-10670 244 8 20 20 NUM ct-10670 244 9 . . PUNCT ct-10670 245 1 cuervo cuervo PROPN ct-10670 245 2 - - PUNCT ct-10670 245 3 arango arango PROPN ct-10670 245 4 j j PROPN ct-10670 245 5 , , PUNCT ct-10670 245 6 claes claes PROPN ct-10670 245 7 an an PRON ct-10670 245 8 , , PUNCT ct-10670 245 9 stout stout ADJ ct-10670 245 10 tae tae PROPN ct-10670 245 11 : : PUNCT ct-10670 245 12 mare mare NOUN ct-10670 245 13 and and CCONJ ct-10670 245 14 stallion stallion NOUN ct-10670 245 15 effects effect NOUN ct-10670 245 16 on on ADP ct-10670 245 17 blastocyst blastocyst NOUN ct-10670 245 18 production production NOUN ct-10670 245 19 in in ADP ct-10670 245 20 a a DET ct-10670 245 21 commercial commercial ADJ ct-10670 245 22 equine equine NOUN ct-10670 245 23 ovum ovum NOUN ct-10670 245 24 pick pick VERB ct-10670 245 25 - - PUNCT ct-10670 245 26 up up ADP ct-10670 245 27 - - PUNCT ct-10670 245 28 intracytoplasmic intracytoplasmic ADJ ct-10670 245 29 sperm sperm NOUN ct-10670 245 30 injection injection NOUN ct-10670 245 31 program program NOUN ct-10670 245 32 . . PUNCT ct-10670 246 1 reprod reprod PROPN ct-10670 246 2 fertil fertil AUX ct-10670 246 3 dev dev PROPN ct-10670 246 4 2019;31:1894 2019;31:1894 PROPN ct-10670 246 5 - - PUNCT ct-10670 246 6 1903 1903 NUM ct-10670 246 7 . . PUNCT ct-10670 247 1 doi doi NOUN ct-10670 247 2 : : PUNCT ct-10670 247 3 10.1071 10.1071 NUM ct-10670 247 4 / / SYM ct-10670 247 5 rd19201 rd19201 PROPN ct-10670 247 6 21 21 NUM ct-10670 247 7 . . PUNCT ct-10670 248 1 orellana orellana PROPN ct-10670 248 2 - - PROPN ct-10670 248 3 guerrero guerrero PROPN ct-10670 248 4 d d PROPN ct-10670 248 5 , , PUNCT ct-10670 248 6 dini dini NOUN ct-10670 248 7 p p NOUN ct-10670 248 8 , , PUNCT ct-10670 248 9 santos santos PROPN ct-10670 248 10 e e NOUN ct-10670 248 11 , , PUNCT ct-10670 248 12 et et PROPN ct-10670 248 13 al al PROPN ct-10670 248 14 : : PUNCT ct-10670 248 15 effect effect NOUN ct-10670 248 16 of of ADP ct-10670 248 17 transvaginal transvaginal ADJ ct-10670 248 18 aspiration aspiration NOUN ct-10670 248 19 of of ADP ct-10670 248 20 oocytes oocyte NOUN ct-10670 248 21 on on ADP ct-10670 248 22 blood blood NOUN ct-10670 248 23 and and CCONJ ct-10670 248 24 peritoneal peritoneal ADJ ct-10670 248 25 fluid fluid ADJ ct-10670 248 26 parameters parameter NOUN ct-10670 248 27 in in ADP ct-10670 248 28     SPACE ct-10670 248 29 mares mare NOUN ct-10670 248 30 . . PUNCT ct-10670 249 1 j j PROPN ct-10670 249 2 equine equine PROPN ct-10670 249 3 vet vet NOUN ct-10670 249 4 sci sci PROPN ct-10670 249 5 2022;114:103949 2022;114:103949 NUM ct-10670 249 6 . . PUNCT ct-10670 250 1 doi doi NOUN ct-10670 250 2 : : PUNCT ct-10670 250 3 10.1016/ 10.1016/ NUM ct-10670 250 4 j.jevs.2022.103949 j.jevs.2022.103949 NOUN ct-10670 250 5 22 22 NUM ct-10670 250 6 . . PUNCT ct-10670 250 7 de de PROPN ct-10670 250 8 la la PROPN ct-10670 250 9 fuente fuente X ct-10670 250 10 a a DET ct-10670 250 11 , , PUNCT ct-10670 250 12 martin martin PROPN ct-10670 250 13 - - PUNCT ct-10670 250 14 pelaez pelaez PROPN ct-10670 250 15 s s PROPN ct-10670 250 16 , , PUNCT ct-10670 250 17 megehee megehee PROPN ct-10670 250 18 s s PROPN ct-10670 250 19 , , PUNCT ct-10670 250 20 et et PROPN ct-10670 250 21 al al PROPN ct-10670 250 22 : : PUNCT ct-10670 250 23 evaluating evaluate VERB ct-10670 250 24 equine equine NOUN ct-10670 250 25 oocyte oocyte ADJ ct-10670 250 26 transportation transportation NOUN ct-10670 250 27 techniques technique NOUN ct-10670 250 28 : : PUNCT ct-10670 250 29 a a DET ct-10670 250 30 comparative comparative ADJ ct-10670 250 31 study study NOUN ct-10670 250 32 of of ADP ct-10670 250 33 maturation maturation NOUN ct-10670 250 34 , , PUNCT ct-10670 250 35 fertilization fertilization NOUN ct-10670 250 36 , , PUNCT ct-10670 250 37 and and CCONJ ct-10670 250 38 blastocyst blastocyst NOUN ct-10670 250 39 rates rate NOUN ct-10670 250 40 in in ADP ct-10670 250 41 equitainer equitainer NOUN ct-10670 250 42 , , PUNCT ct-10670 250 43 microq microq NOUN ct-10670 250 44 , , PUNCT ct-10670 250 45 and and CCONJ ct-10670 250 46 incubator incubator NOUN ct-10670 250 47 systems system NOUN ct-10670 250 48 . . PUNCT ct-10670 251 1 in in ADP ct-10670 251 2 : : PUNCT ct-10670 251 3 demetrio demetrio PROPN ct-10670 251 4 d d PROPN ct-10670 251 5 , , PUNCT ct-10670 251 6 tesfaye tesfaye VERB ct-10670 251 7 d d NOUN ct-10670 251 8 : : PUNCT ct-10670 251 9 editors editor NOUN ct-10670 251 10 . . PUNCT ct-10670 252 1 reprod reprod PROPN ct-10670 252 2 fertil fertil PROPN ct-10670 252 3 dev dev PROPN ct-10670 252 4 2024:36 2024:36 NUM ct-10670 252 5 . . PUNCT ct-10670 253 1 clayton clayton PROPN ct-10670 253 2 : : PUNCT ct-10670 253 3 proc proc PROPN ct-10670 253 4 iets iets PROPN ct-10670 253 5 csiro csiro PROPN ct-10670 253 6 publishing publishing NOUN ct-10670 253 7 ; ; PUNCT ct-10670 253 8 2024:1 2024:1 NUM ct-10670 253 9 - - SYM ct-10670 253 10 274 274 NUM ct-10670 253 11 . . PUNCT ct-10670 253 12 23 23 NUM ct-10670 253 13 . . PUNCT ct-10670 254 1 agnieszka agnieszka PROPN ct-10670 254 2 n n CCONJ ct-10670 254 3 , , PUNCT ct-10670 254 4 joanna joanna PROPN ct-10670 254 5 k k PROPN ct-10670 254 6 , , PUNCT ct-10670 254 7 wojciech wojciech PROPN ct-10670 254 8 w w PROPN ct-10670 254 9 , , PUNCT ct-10670 254 10 et et PROPN ct-10670 254 11 al al PROPN ct-10670 254 12 : : PUNCT ct-10670 254 13 in in ADP ct-10670 254 14 vitro vitro X ct-10670 254 15 maturation maturation NOUN ct-10670 254 16 of of ADP ct-10670 254 17     SPACE ct-10670 254 18 equine equine NOUN ct-10670 254 19 oocytes oocyte NOUN ct-10670 254 20 followed follow VERB ct-10670 254 21 by by ADP ct-10670 254 22 two two NUM ct-10670 254 23 vitrification vitrification NOUN ct-10670 254 24 protocols protocol NOUN ct-10670 254 25 and and CCONJ ct-10670 254 26 subjected subject VERB ct-10670 254 27 to to ADP ct-10670 254 28 either either CCONJ ct-10670 254 29 intracytoplasmic intracytoplasmic ADJ ct-10670 254 30 sperm sperm NOUN ct-10670 254 31 injection injection NOUN ct-10670 254 32 ( ( PUNCT ct-10670 254 33 icsi icsi NOUN ct-10670 254 34 ) ) PUNCT ct-10670 254 35 or or CCONJ ct-10670 254 36 parthenogenic parthenogenic ADJ ct-10670 254 37 activation activation NOUN ct-10670 254 38 . . PUNCT ct-10670 255 1 theriogenology theriogenology NOUN ct-10670 255 2 2021;162:42 2021;162:42 NUM ct-10670 255 3 - - SYM ct-10670 255 4 48 48 NUM ct-10670 255 5 . . PUNCT ct-10670 256 1 doi doi NOUN ct-10670 256 2 : : PUNCT ct-10670 256 3 10.1016 10.1016 NUM ct-10670 256 4 / / SYM ct-10670 256 5 j.theriogenology.2020.12.022 j.theriogenology.2020.12.022 PROPN ct-10670 256 6 24 24 NUM ct-10670 256 7 . . PUNCT ct-10670 257 1 maclellan maclellan PROPN ct-10670 257 2 lj lj PROPN ct-10670 257 3 , , PUNCT ct-10670 257 4 albertini albertini PROPN ct-10670 257 5 df df PROPN ct-10670 257 6 , , PUNCT ct-10670 257 7 stokes stokes PROPN ct-10670 257 8 je je PROPN ct-10670 257 9 , , PUNCT ct-10670 257 10 et et PROPN ct-10670 257 11 al al PROPN ct-10670 257 12 : : PUNCT ct-10670 257 13 use use NOUN ct-10670 257 14 of of ADP ct-10670 257 15 confocal confocal ADJ ct-10670 257 16 microscopy microscopy NOUN ct-10670 257 17 and and CCONJ ct-10670 257 18 intracytoplasmic intracytoplasmic ADJ ct-10670 257 19 sperm sperm NOUN ct-10670 257 20 injection injection NOUN ct-10670 257 21 ( ( PUNCT ct-10670 257 22 icsi icsi NOUN ct-10670 257 23 ) ) PUNCT ct-10670 257 24 to to PART ct-10670 257 25 assess assess VERB ct-10670 257 26 viability viability NOUN ct-10670 257 27 of of ADP ct-10670 257 28 equine equine NOUN ct-10670 257 29 oocytes oocyte NOUN ct-10670 257 30 from from ADP ct-10670 257 31 young young ADJ ct-10670 257 32 and and CCONJ ct-10670 257 33 old old ADJ ct-10670 257 34 mares mare NOUN ct-10670 257 35 after after ADP ct-10670 257 36 vitrification vitrification NOUN ct-10670 257 37 . . PUNCT ct-10670 258 1 j j PROPN ct-10670 258 2 assist assist VERB ct-10670 258 3 reprod reprod PROPN ct-10670 258 4 genet genet PROPN ct-10670 258 5 2023;40:2565 2023;40:2565 PROPN ct-10670 258 6 - - SYM ct-10670 258 7 2576 2576 NUM ct-10670 258 8 . . PUNCT ct-10670 259 1 doi doi NOUN ct-10670 259 2 : : PUNCT ct-10670 259 3 10.1007/ 10.1007/ NUM ct-10670 259 4 s10815 s10815 PROPN ct-10670 259 5 - - PUNCT ct-10670 259 6 023 023 NUM ct-10670 259 7 - - PUNCT ct-10670 259 8 02935 02935 NUM ct-10670 259 9 - - SYM ct-10670 259 10 4 4 NUM ct-10670 259 11 25 25 NUM ct-10670 259 12 . . PUNCT ct-10670 260 1 angel angel NOUN ct-10670 260 2 - - PUNCT ct-10670 260 3 velez velez PROPN ct-10670 260 4 d d PROPN ct-10670 260 5 , , PUNCT ct-10670 260 6 meese meese PROPN ct-10670 260 7 t t PROPN ct-10670 260 8 , , PUNCT ct-10670 260 9 hedia hedia PROPN ct-10670 260 10 m m PROPN ct-10670 260 11 , , PUNCT ct-10670 260 12 et et PROPN ct-10670 260 13 al al PROPN ct-10670 260 14 : : PUNCT ct-10670 260 15 transcriptomics transcriptomic NOUN ct-10670 260 16 reveal reveal VERB ct-10670 260 17 molecular molecular ADJ ct-10670 260 18 differences difference NOUN ct-10670 260 19 in in ADP ct-10670 260 20 equine equine NOUN ct-10670 260 21 oocytes oocyte NOUN ct-10670 260 22 vitrified vitrify VERB ct-10670 260 23 before before ADV ct-10670 260 24 and and CCONJ ct-10670 260 25 after after ADP ct-10670 260 26 in in ADP ct-10670 260 27 vitro vitro X ct-10670 260 28 maturation maturation NOUN ct-10670 260 29 . . PUNCT ct-10670 261 1 int int PROPN ct-10670 261 2 j j PROPN ct-10670 261 3 mol mol ADP ct-10670 261 4 sci sci PROPN ct-10670 261 5 2023;24(8):6915 2023;24(8):6915 PROPN ct-10670 261 6 . . PUNCT ct-10670 262 1 doi doi NOUN ct-10670 262 2 : : PUNCT ct-10670 262 3 10.3390/ 10.3390/ NUM ct-10670 262 4 ijms24086915 ijms24086915 PROPN ct-10670 262 5 26 26 NUM ct-10670 262 6 . . PUNCT ct-10670 263 1 ortiz ortiz PROPN ct-10670 263 2 - - PUNCT ct-10670 263 3 escribano escribano PROPN ct-10670 263 4 n n CCONJ ct-10670 263 5 , , PUNCT ct-10670 263 6 bogado bogado NOUN ct-10670 263 7 pascottini pascottini NOUN ct-10670 263 8 o o NOUN ct-10670 263 9 , , PUNCT ct-10670 263 10 woelders woelder VERB ct-10670 263 11 h h NOUN ct-10670 263 12 , , PUNCT ct-10670 263 13 et et PROPN ct-10670 263 14 al al PROPN ct-10670 263 15 : : PUNCT ct-10670 263 16 an an DET ct-10670 263 17 improved improved ADJ ct-10670 263 18 vitrification vitrification NOUN ct-10670 263 19 protocol protocol NOUN ct-10670 263 20 for for ADP ct-10670 263 21 equine equine NOUN ct-10670 263 22 immature immature ADJ ct-10670 263 23 oocytes oocyte NOUN ct-10670 263 24 , , PUNCT ct-10670 263 25 resulting result VERB ct-10670 263 26 in in ADP ct-10670 263 27 a a DET ct-10670 263 28 first first ADJ ct-10670 263 29 live live ADJ ct-10670 263 30 foal foal NOUN ct-10670 263 31 . . PUNCT ct-10670 264 1 equine equine NOUN ct-10670 264 2 vet vet NOUN ct-10670 264 3 j j PROPN ct-10670 264 4 2018;50:391 2018;50:391 NUM ct-10670 264 5 - - SYM ct-10670 264 6 397 397 NUM ct-10670 264 7 . . PUNCT ct-10670 265 1 doi doi NOUN ct-10670 265 2 : : PUNCT ct-10670 265 3 10.1111 10.1111 NUM ct-10670 265 4 / / SYM ct-10670 265 5 evj.12747 evj.12747 NUM ct-10670 265 6 27 27 NUM ct-10670 265 7 . . PUNCT ct-10670 265 8 de de X ct-10670 265 9 la la PROPN ct-10670 265 10 fuente fuente PROPN ct-10670 265 11 a a PRON ct-10670 265 12 , , PUNCT ct-10670 265 13 scoggin scoggin NOUN ct-10670 265 14 c c NOUN ct-10670 265 15 , , PUNCT ct-10670 265 16 bradecamp bradecamp ADJ ct-10670 265 17 e e NOUN ct-10670 265 18 , , PUNCT ct-10670 265 19 et et PROPN ct-10670 265 20 al al PROPN ct-10670 265 21 : : PUNCT ct-10670 265 22 transcriptome transcriptome ADJ ct-10670 265 23 signature signature NOUN ct-10670 265 24 of of ADP ct-10670 265 25 immature immature ADJ ct-10670 265 26 and and CCONJ ct-10670 265 27 in in ADP ct-10670 265 28 vitro vitro X ct-10670 265 29 - - PUNCT ct-10670 265 30 matured mature VERB ct-10670 265 31 equine equine NOUN ct-10670 265 32 cumulus cumulus NOUN ct-10670 265 33 - - PUNCT ct-10670 265 34 oocytes oocyte NOUN ct-10670 265 35 complex complex NOUN ct-10670 265 36 . . PUNCT ct-10670 266 1 int int PROPN ct-10670 266 2 j j PROPN ct-10670 266 3 mol mol ADP ct-10670 266 4 sci sci PROPN ct-10670 266 5 2023;24(18):13718 2023;24(18):13718 PROPN ct-10670 266 6 . . PUNCT ct-10670 266 7 doi doi PROPN ct-10670 266 8 : : PUNCT ct-10670 266 9 10.3390/ 10.3390/ NUM ct-10670 266 10 ijms241813718 ijms241813718 PROPN ct-10670 266 11 28 28 NUM ct-10670 266 12 . . PUNCT ct-10670 267 1 martin martin PROPN ct-10670 267 2 - - PUNCT ct-10670 267 3 pelaez pelaez PROPN ct-10670 267 4 s s PART ct-10670 267 5 , , PUNCT ct-10670 267 6 de de X ct-10670 267 7 la la X ct-10670 267 8 fuente fuente X ct-10670 267 9 a a X ct-10670 267 10 , , PUNCT ct-10670 267 11 meyers meyers PROPN ct-10670 267 12 s s PART ct-10670 267 13 , , PUNCT ct-10670 267 14 et et PROPN ct-10670 267 15 al al PROPN ct-10670 267 16 : : PUNCT ct-10670 267 17 capturing capture VERB ct-10670 267 18 the the DET ct-10670 267 19 miracle miracle NOUN ct-10670 267 20 : : PUNCT ct-10670 267 21 time time NOUN ct-10670 267 22 - - PUNCT ct-10670 267 23 lapse lapse NOUN ct-10670 267 24 imaging imaging NOUN ct-10670 267 25 of of ADP ct-10670 267 26 equine equine NOUN ct-10670 267 27 embryos embryo NOUN ct-10670 267 28 reveals reveal VERB ct-10670 267 29 cleavage cleavage NOUN ct-10670 267 30 patterns pattern NOUN ct-10670 267 31 impact impact NOUN ct-10670 267 32 pregnancy pregnancy NOUN ct-10670 267 33 success success NOUN ct-10670 267 34 . . PUNCT ct-10670 268 1 in in ADP ct-10670 268 2 : : PUNCT ct-10670 268 3 demetrio demetrio PROPN ct-10670 268 4 d d PROPN ct-10670 268 5 , , PUNCT ct-10670 268 6 tesfaye tesfaye VERB ct-10670 268 7 d d NOUN ct-10670 268 8 : : PUNCT ct-10670 268 9 editors editor NOUN ct-10670 268 10 . . PUNCT ct-10670 269 1 reprod reprod PROPN ct-10670 269 2 fertil fertil PROPN ct-10670 269 3 dev dev PROPN ct-10670 269 4 2024:36 2024:36 NUM ct-10670 269 5 . . PUNCT ct-10670 270 1 proc proc NOUN ct-10670 270 2 iets iets PROPN ct-10670 270 3 csiro csiro PROPN ct-10670 270 4 ; ; PUNCT ct-10670 270 5 2024:1 2024:1 NUM ct-10670 270 6 - - SYM ct-10670 270 7 274 274 NUM ct-10670 270 8 . . PUNCT ct-10670 270 9 available available ADJ ct-10670 270 10 from from ADP ct-10670 270 11 : : PUNCT ct-10670 270 12 https://www.publish.csiro.au/rd/rdv36n2ab98 https://www.publish.csiro.au/rd/rdv36n2ab98 NUM ct-10670 270 13 [ [ X ct-10670 270 14 cited cite VERB ct-10670 270 15 26 26 NUM ct-10670 270 16 january january NOUN ct-10670 270 17 2024 2024 NUM ct-10670 270 18 ] ] PUNCT ct-10670 270 19 . . PUNCT ct-10670 271 1 29 29 NUM ct-10670 271 2 . . PUNCT ct-10670 272 1 burruel burruel PROPN ct-10670 272 2 v v PROPN ct-10670 272 3 , , PUNCT ct-10670 272 4 klooster klooster PROPN ct-10670 272 5 k k PROPN ct-10670 272 6 , , PUNCT ct-10670 272 7 barker barker PROPN ct-10670 272 8 cm cm PROPN ct-10670 272 9 , , PUNCT ct-10670 272 10 et et PROPN ct-10670 272 11 al al PROPN ct-10670 272 12 : : PUNCT ct-10670 272 13 abnormal abnormal ADJ ct-10670 272 14 early early ADJ ct-10670 272 15 cleavage cleavage NOUN ct-10670 272 16 events event NOUN ct-10670 272 17 predict predict VERB ct-10670 272 18 early early ADJ ct-10670 272 19 embryo embryo NOUN ct-10670 272 20 demise demise NOUN ct-10670 272 21 : : PUNCT ct-10670 272 22 sperm sperm NOUN ct-10670 272 23 oxidative oxidative ADJ ct-10670 272 24 stress stress NOUN ct-10670 272 25 and and CCONJ ct-10670 272 26 early early ADJ ct-10670 272 27 abnormal abnormal ADJ ct-10670 272 28 cleavage cleavage NOUN ct-10670 272 29 . . PUNCT ct-10670 273 1 sci sci PROPN ct-10670 273 2 rep rep PROPN ct-10670 273 3 2014;4:6598 2014;4:6598 PROPN ct-10670 273 4 . . PUNCT ct-10670 274 1 doi doi NOUN ct-10670 274 2 : : PUNCT ct-10670 274 3 10.1038/ 10.1038/ NUM ct-10670 274 4 srep06598 srep06598 PROPN ct-10670 274 5 30 30 NUM ct-10670 274 6 . . PUNCT ct-10670 274 7 de de PROPN ct-10670 274 8 la la PROPN ct-10670 274 9 fuente fuente X ct-10670 274 10 a a DET ct-10670 274 11 , , PUNCT ct-10670 274 12 omyla omyla ADJ ct-10670 274 13 k k PROPN ct-10670 274 14 , , PUNCT ct-10670 274 15 cooper cooper PROPN ct-10670 274 16 c c PROPN ct-10670 274 17 , , PUNCT ct-10670 274 18 et et PROPN ct-10670 274 19 al al PROPN ct-10670 274 20 : : PUNCT ct-10670 274 21 embryo embryo NOUN ct-10670 274 22 pulsing pulsing NOUN ct-10670 274 23 : : PUNCT ct-10670 274 24 repeated repeat VERB ct-10670 274 25 expansion expansion NOUN ct-10670 274 26 and and CCONJ ct-10670 274 27 contraction contraction NOUN ct-10670 274 28 of of ADP ct-10670 274 29 in in ADP ct-10670 274 30 vivo vivo NOUN ct-10670 274 31 and and CCONJ ct-10670 274 32 in in ADP ct-10670 274 33 vitro vitro X ct-10670 274 34 equine equine NOUN ct-10670 274 35 blastocysts blastocyst NOUN ct-10670 274 36 . . PUNCT ct-10670 275 1 j j PROPN ct-10670 275 2 equine equine PROPN ct-10670 275 3 vet vet NOUN ct-10670 275 4 sci sci PROPN ct-10670 275 5 2023;128:104891 2023;128:104891 NUM ct-10670 275 6 . . PUNCT ct-10670 276 1 doi doi NOUN ct-10670 276 2 : : PUNCT ct-10670 276 3 10.1016/ 10.1016/ NUM ct-10670 276 4 j.jevs.2023.104891 j.jevs.2023.104891 NOUN ct-10670 276 5 31 31 NUM ct-10670 276 6 . . PUNCT ct-10670 277 1 gazzo gazzo PROPN ct-10670 277 2 e e PROPN ct-10670 277 3 , , PUNCT ct-10670 277 4 peña peña PROPN ct-10670 277 5 f f PROPN ct-10670 277 6 , , PUNCT ct-10670 277 7 valdéz valdéz PROPN ct-10670 277 8 f f PROPN ct-10670 277 9 , , PUNCT ct-10670 277 10 et et PROPN ct-10670 277 11 al al PROPN ct-10670 277 12 : : PUNCT ct-10670 277 13 blastocyst blastocyst NOUN ct-10670 277 14 contractions contraction NOUN ct-10670 277 15 are be AUX ct-10670 277 16 strongly strongly ADV ct-10670 277 17 related relate VERB ct-10670 277 18 with with ADP ct-10670 277 19 aneuploidy aneuploidy ADJ ct-10670 277 20 , , PUNCT ct-10670 277 21 lower low ADJ ct-10670 277 22 implantation implantation NOUN ct-10670 277 23 rates rate NOUN ct-10670 277 24 , , PUNCT ct-10670 277 25 and and CCONJ ct-10670 277 26 slow slow ADV ct-10670 277 27 - - PUNCT ct-10670 277 28 cleaving cleave VERB ct-10670 277 29 embryos embryos NOUN ct-10670 277 30 : : PUNCT ct-10670 277 31 a a DET ct-10670 277 32 time time NOUN ct-10670 277 33 lapse lapse NOUN ct-10670 277 34 study study NOUN ct-10670 277 35 . . PUNCT ct-10670 278 1 jbra jbra NOUN ct-10670 278 2 assist assist VERB ct-10670 278 3 reprod reprod NOUN ct-10670 279 1 2020;24:7781 2020;24:7781 NUM ct-10670 279 2 . . PUNCT ct-10670 279 3 doi doi NOUN ct-10670 279 4 : : PUNCT ct-10670 279 5 10.5935/1518 10.5935/1518 ADJ ct-10670 279 6 - - PUNCT ct-10670 279 7 0557.20190053 0557.20190053 ADJ ct-10670 279 8 32 32 NUM ct-10670 279 9 . . PUNCT ct-10670 280 1 caprell caprell PROPN ct-10670 280 2 h h NOUN ct-10670 280 3 , , PUNCT ct-10670 280 4 connerney connerney PROPN ct-10670 280 5 m m PROPN ct-10670 280 6 , , PUNCT ct-10670 280 7 boylan boylan PROPN ct-10670 280 8 c c PROPN ct-10670 280 9 , , PUNCT ct-10670 280 10 et et PROPN ct-10670 280 11 al al PROPN ct-10670 280 12 : : PUNCT ct-10670 280 13 increased increase VERB ct-10670 280 14 expansion expansion NOUN ct-10670 280 15 and and CCONJ ct-10670 280 16 decreased decrease VERB ct-10670 280 17 contraction contraction NOUN ct-10670 280 18 of of ADP ct-10670 280 19 embryos embryo NOUN ct-10670 280 20 corresponds correspond VERB ct-10670 280 21 to to ADP ct-10670 280 22 increased increase VERB ct-10670 280 23 clinical clinical ADJ ct-10670 280 24 pregnancy pregnancy NOUN ct-10670 280 25 rates rate NOUN ct-10670 280 26 in in ADP ct-10670 280 27 single single ADJ ct-10670 280 28 fet fet PROPN ct-10670 280 29 cycles cycles PROPN ct-10670 280 30 . . PUNCT ct-10670 281 1 reprod reprod PROPN ct-10670 281 2 biomed biome VERB ct-10670 281 3 online online ADJ ct-10670 281 4 2019;39 2019;39 NUM ct-10670 281 5 : : PUNCT ct-10670 281 6 e11 e11 ADJ ct-10670 281 7 - - PUNCT ct-10670 281 8 e12 e12 NOUN ct-10670 281 9 . . PUNCT ct-10670 282 1 doi doi NOUN ct-10670 282 2 : : PUNCT ct-10670 282 3 10.1016 10.1016 NUM ct-10670 282 4 / / SYM ct-10670 282 5 j.rbmo.2019.07.023 j.rbmo.2019.07.023 PROPN ct-10670 282 6 33 33 NUM ct-10670 282 7 . . PUNCT ct-10670 283 1 sugimura sugimura PROPN ct-10670 283 2 s s PROPN ct-10670 283 3 , , PUNCT ct-10670 283 4 akai akai PROPN ct-10670 283 5 t t PROPN ct-10670 283 6 , , PUNCT ct-10670 283 7 imai imai PROPN ct-10670 283 8 k k PROPN ct-10670 283 9 : : PUNCT ct-10670 283 10 selection selection NOUN ct-10670 283 11 of of ADP ct-10670 283 12 viable viable ADJ ct-10670 283 13 in in ADP ct-10670 283 14 vitro vitro X ct-10670 283 15 - - PUNCT ct-10670 283 16 fertilized fertilize VERB ct-10670 283 17 bovine bovine ADJ ct-10670 283 18 embryos embryo NOUN ct-10670 283 19 using use VERB ct-10670 283 20 time time NOUN ct-10670 283 21 - - PUNCT ct-10670 283 22 lapse lapse NOUN ct-10670 283 23 monitoring monitoring NOUN ct-10670 283 24 in in ADP ct-10670 283 25 microwell microwell NOUN ct-10670 283 26 culture culture NOUN ct-10670 283 27 dishes dish NOUN ct-10670 283 28 . . PUNCT ct-10670 284 1 j j PROPN ct-10670 284 2 reprod reprod PROPN ct-10670 284 3 dev dev PROPN ct-10670 284 4 2017;63:353 2017;63:353 PROPN ct-10670 284 5 - - PROPN ct-10670 284 6 357 357 NUM ct-10670 284 7 . . PUNCT ct-10670 285 1 doi doi NOUN ct-10670 285 2 : : PUNCT ct-10670 285 3 10.1262/ 10.1262/ NUM ct-10670 285 4 jrd.2017 jrd.2017 NOUN ct-10670 285 5 - - NOUN ct-10670 285 6 041 041 NUM ct-10670 285 7 34 34 NUM ct-10670 285 8 . . PUNCT ct-10670 286 1 lewis lewis PROPN ct-10670 286 2 n n PROPN ct-10670 286 3 , , PUNCT ct-10670 286 4 schnauffer schnauffer PROPN ct-10670 286 5 k k PROPN ct-10670 286 6 , , PUNCT ct-10670 286 7 hinrichs hinrichs PROPN ct-10670 286 8 k k PROPN ct-10670 286 9 , , PUNCT ct-10670 286 10 et et PROPN ct-10670 286 11 al al PROPN ct-10670 286 12 : : PUNCT ct-10670 286 13 morphokinetics morphokinetic NOUN ct-10670 286 14 of of ADP ct-10670 286 15 early early ADJ ct-10670 286 16 equine equine NOUN ct-10670 286 17 embryo embryo NOUN ct-10670 286 18 development development NOUN ct-10670 286 19 in in ADP ct-10670 286 20 vitro vitro X ct-10670 286 21 using use VERB ct-10670 286 22 time time NOUN ct-10670 286 23 - - PUNCT ct-10670 286 24 lapse lapse NOUN ct-10670 286 25 imaging imaging NOUN ct-10670 286 26 , , PUNCT ct-10670 286 27 and and CCONJ ct-10670 286 28 use use NOUN ct-10670 286 29 in in ADP ct-10670 286 30 selecting select VERB ct-10670 286 31 blastocysts blastocyst NOUN ct-10670 286 32 for for ADP ct-10670 286 33 transfer transfer NOUN ct-10670 286 34 . . PUNCT ct-10670 287 1 reprod reprod PROPN ct-10670 287 2 fertil fertil AUX ct-10670 287 3 dev dev PROPN ct-10670 287 4 2019;31:1851 2019;31:1851 PROPN ct-10670 287 5 - - SYM ct-10670 287 6 1861 1861 NUM ct-10670 287 7 . . PUNCT ct-10670 288 1 doi doi NOUN ct-10670 288 2 : : PUNCT ct-10670 288 3 10.1071 10.1071 NUM ct-10670 288 4 / / SYM ct-10670 288 5 rd19225 rd19225 PROPN ct-10670 288 6 35 35 NUM ct-10670 288 7 . . PUNCT ct-10670 289 1 lewis lewis PROPN ct-10670 289 2 n n PROPN ct-10670 289 3 , , PUNCT ct-10670 289 4 canesin canesin NOUN ct-10670 289 5 h h NOUN ct-10670 289 6 , , PUNCT ct-10670 289 7 choi choi PROPN ct-10670 289 8 yh yh PROPN ct-10670 289 9 , , PUNCT ct-10670 289 10 et et PROPN ct-10670 289 11 al al PROPN ct-10670 289 12 : : PUNCT ct-10670 289 13 equine equine NOUN ct-10670 289 14 in in ADP ct-10670 289 15 vitro vitro X ct-10670 289 16 produced produce VERB ct-10670 289 17 blastocysts blastocyst NOUN ct-10670 289 18 : : PUNCT ct-10670 289 19 relationship relationship NOUN ct-10670 289 20 of of ADP ct-10670 289 21 embryo embryo NOUN ct-10670 289 22 morphology morphology NOUN ct-10670 289 23 , , PUNCT ct-10670 289 24 stage stage NOUN ct-10670 289 25 and and CCONJ ct-10670 289 26 speed speed NOUN ct-10670 289 27     SPACE ct-10670 289 28 of of ADP ct-10670 289 29 development development NOUN ct-10670 289 30 to to ADP ct-10670 289 31 foaling foal VERB ct-10670 289 32 rate rate NOUN ct-10670 289 33 . . PUNCT ct-10670 290 1 reprod reprod PROPN ct-10670 290 2 fertil fertil PROPN ct-10670 290 3 dev dev PROPN ct-10670 290 4 2023;35:338 2023;35:338 PROPN ct-10670 290 5 - - PUNCT ct-10670 290 6 351 351 NUM ct-10670 290 7 . . PUNCT ct-10670 291 1 doi doi NOUN ct-10670 291 2 : : PUNCT ct-10670 291 3 10.1071 10.1071 NUM ct-10670 291 4 / / SYM ct-10670 291 5 rd22224 rd22224 NOUN ct-10670 291 6 36 36 NUM ct-10670 291 7 . . PUNCT ct-10670 292 1 iqbal iqbal PROPN ct-10670 292 2 k k PROPN ct-10670 292 3 , , PUNCT ct-10670 292 4 chitwood chitwood PROPN ct-10670 292 5 jl jl PROPN ct-10670 292 6 , , PUNCT ct-10670 292 7 meyers meyer NOUN ct-10670 292 8 - - PUNCT ct-10670 292 9 brown brown PROPN ct-10670 292 10 ga ga PROPN ct-10670 292 11 , , PUNCT ct-10670 292 12 et et PROPN ct-10670 292 13 al al PROPN ct-10670 292 14 : : PUNCT ct-10670 292 15 rna rna NOUN ct-10670 292 16 - - PUNCT ct-10670 292 17 seq seq NOUN ct-10670 292 18 transcriptome transcriptome ADJ ct-10670 292 19 profiling profiling NOUN ct-10670 292 20 of of ADP ct-10670 292 21 equine equine NOUN ct-10670 292 22 inner inner ADJ ct-10670 292 23 cell cell NOUN ct-10670 292 24 mass mass NOUN ct-10670 292 25 and and CCONJ ct-10670 292 26 trophectoderm trophectoderm NOUN ct-10670 292 27 . . PUNCT ct-10670 293 1 biol biol PROPN ct-10670 293 2 reprod reprod PROPN ct-10670 293 3 2014;90:61 2014;90:61 NUM ct-10670 293 4 . . PUNCT ct-10670 294 1 doi doi NOUN ct-10670 294 2 : : PUNCT ct-10670 294 3 10.1095 10.1095 NUM ct-10670 294 4 / / SYM ct-10670 294 5 biolreprod.113.113928 biolreprod.113.113928 ADP ct-10670 294 6 37 37 NUM ct-10670 294 7 . . PUNCT ct-10670 295 1 chitwood chitwood PROPN ct-10670 295 2 jl jl PROPN ct-10670 295 3 , , PUNCT ct-10670 295 4 burruel burruel PROPN ct-10670 295 5 vr vr PROPN ct-10670 295 6 , , PUNCT ct-10670 295 7 halstead halstead PROPN ct-10670 295 8 mm mm PROPN ct-10670 295 9 , , PUNCT ct-10670 295 10 et et PROPN ct-10670 295 11 al al PROPN ct-10670 295 12 : : PUNCT ct-10670 295 13 transcriptome transcriptome ADJ ct-10670 295 14 profiling profiling NOUN ct-10670 295 15 of of ADP ct-10670 295 16 individual individual ADJ ct-10670 295 17 rhesus rhesus NOUN ct-10670 295 18 macaque macaque ADJ ct-10670 295 19 oocytes oocyte NOUN ct-10670 295 20 and and CCONJ ct-10670 295 21 preimplantation preimplantation NOUN ct-10670 295 22 embryos embryo NOUN ct-10670 295 23 . . PUNCT ct-10670 296 1 biol biol PROPN ct-10670 296 2 reprod reprod NOUN ct-10670 296 3 2017;97:353 2017;97:353 NUM ct-10670 296 4 - - SYM ct-10670 296 5 364 364 NUM ct-10670 296 6 . . PUNCT ct-10670 296 7 doi doi NOUN ct-10670 296 8 : : PUNCT ct-10670 296 9 10.1093 10.1093 NUM ct-10670 296 10 / / SYM ct-10670 296 11 biolre biolre PROPN ct-10670 296 12 / / SYM ct-10670 296 13 iox114 iox114 PROPN ct-10670 296 14 http://dx.doi.org/10.58292/ct.v16.10670 http://dx.doi.org/10.58292/ct.v16.10670 PROPN ct-10670 296 15 https://doi.org/10.1111/evj.12159 https://doi.org/10.1111/evj.12159 PROPN ct-10670 296 16 https://doi.org/10.1111/evj.12159 https://doi.org/10.1111/evj.12159 PROPN ct-10670 296 17 https://doi.org/10.1016/j.jevs.2020.103097 https://doi.org/10.1016/j.jevs.2020.103097 PROPN ct-10670 296 18 https://doi.org/10.1016/j.jevs.2020.103097 https://doi.org/10.1016/j.jevs.2020.103097 ADJ ct-10670 296 19 https://doi.org/10.1071/rd19260 https://doi.org/10.1071/rd19260 PROPN ct-10670 296 20 https://doi.org/10.1093/biolre/ioac172 https://doi.org/10.1093/biolre/ioac172 X ct-10670 296 21 https://doi.org/10.1071/rd19249 https://doi.org/10.1071/rd19249 PRON ct-10670 296 22 https://doi.org/10.1016/j.jevs.2023.104660 https://doi.org/10.1016/j.jevs.2023.104660 VERB ct-10670 296 23 https://doi.org/10.1016/j.theriogenology.2023.04.002 https://doi.org/10.1016/j.theriogenology.2023.04.002 NOUN ct-10670 296 24 https://doi.org/10.1016/j.theriogenology.2023.04.002 https://doi.org/10.1016/j.theriogenology.2023.04.002 NOUN ct-10670 296 25 https://doi.org/10.1016/j.theriogenology.2014.03.025 https://doi.org/10.1016/j.theriogenology.2014.03.025 NOUN ct-10670 296 26 https://doi.org/10.1016/j.theriogenology.2014.03.025 https://doi.org/10.1016/j.theriogenology.2014.03.025 NOUN ct-10670 296 27 https://doi.org/10.1111/j.2042-3306.2012.00606.x https://doi.org/10.1111/j.2042-3306.2012.00606.x ADV ct-10670 296 28 https://doi.org/10.1071/rd19201 https://doi.org/10.1071/rd19201 ADJ ct-10670 296 29 https://doi.org/10.1016/j.jevs.2022.103949 https://doi.org/10.1016/j.jevs.2022.103949 PROPN ct-10670 296 30 https://doi.org/10.1016/j.jevs.2022.103949 https://doi.org/10.1016/j.jevs.2022.103949 PROPN ct-10670 296 31 https://doi.org/10.1016/j.theriogenology.2020.12.022 https://doi.org/10.1016/j.theriogenology.2020.12.022 X ct-10670 296 32 https://doi.org/10.1007/s10815-023-02935-4 https://doi.org/10.1007/s10815-023-02935-4 NUM ct-10670 296 33 https://doi.org/10.1007/s10815-023-02935-4 https://doi.org/10.1007/s10815-023-02935-4 NUM ct-10670 296 34 https://doi.org/10.3390/ijms24086915 https://doi.org/10.3390/ijms24086915 NOUN ct-10670 296 35 https://doi.org/10.3390/ijms24086915 https://doi.org/10.3390/ijms24086915 NOUN ct-10670 296 36 https://doi.org/10.1111/evj.12747 https://doi.org/10.1111/evj.12747 PROPN ct-10670 296 37 https://doi.org/10.3390/ijms241813718 https://doi.org/10.3390/ijms241813718 PROPN ct-10670 296 38 https://doi.org/10.3390/ijms241813718 https://doi.org/10.3390/ijms241813718 PROPN ct-10670 296 39 https://www.publish.csiro.au/rd/rdv36n2ab98 https://www.publish.csiro.au/rd/rdv36n2ab98 NUM ct-10670 296 40 https://doi.org/10.1038/srep06598 https://doi.org/10.1038/srep06598 PROPN ct-10670 296 41 https://doi.org/10.1038/srep06598 https://doi.org/10.1038/srep06598 PROPN ct-10670 296 42 https://doi.org/10.1016/j.jevs.2023.104891 https://doi.org/10.1016/j.jevs.2023.104891 ADJ ct-10670 296 43 https://doi.org/10.1016/j.jevs.2023.104891 https://doi.org/10.1016/j.jevs.2023.104891 PROPN ct-10670 296 44 https://doi.org/10.5935/1518-0557.20190053 https://doi.org/10.5935/1518-0557.20190053 PROPN ct-10670 296 45 https://doi.org/10.1016/j.rbmo.2019.07.023 https://doi.org/10.1016/j.rbmo.2019.07.023 PROPN ct-10670 296 46 https://doi.org/10.1262/jrd.2017-041 https://doi.org/10.1262/jrd.2017-041 NOUN ct-10670 296 47 https://doi.org/10.1262/jrd.2017-041 https://doi.org/10.1262/jrd.2017-041 VERB ct-10670 296 48 https://doi.org/10.1071/rd19225 https://doi.org/10.1071/rd19225 X ct-10670 296 49 https://doi.org/10.1071/rd22224 https://doi.org/10.1071/rd22224 X ct-10670 296 50 https://doi.org/10.1095/biolreprod.113.113928 https://doi.org/10.1095/biolreprod.113.113928 X ct-10670 296 51 https://doi.org/10.1093/biolre/iox114 https://doi.org/10.1093/biolre/iox114 NOUN