id sid tid token lemma pos ct-9406 1 1 1 1 NUM ct-9406 1 2 contact contact NOUN ct-9406 1 3 heriberto heriberto VERB ct-9406 1 4 rodriguez rodriguez PROPN ct-9406 1 5 - - PUNCT ct-9406 1 6 martinez martinez PROPN ct-9406 1 7 heriberto.rodriguez-martinez@liu.se heriberto.rodriguez-martinez@liu.se PROPN ct-9406 2 1 © © PROPN ct-9406 2 2 2023 2023 NUM ct-9406 2 3 the the DET ct-9406 2 4 author(s author(s NOUN ct-9406 2 5 ) ) PUNCT ct-9406 2 6 . . PUNCT ct-9406 3 1 this this PRON ct-9406 3 2 is be AUX ct-9406 3 3 an an DET ct-9406 3 4 open open ADJ ct-9406 3 5 access access NOUN ct-9406 3 6 article article NOUN ct-9406 3 7 distributed distribute VERB ct-9406 3 8 under under ADP ct-9406 3 9 the the DET ct-9406 3 10 terms term NOUN ct-9406 3 11 of of ADP ct-9406 3 12 the the DET ct-9406 3 13 creative creative ADJ ct-9406 3 14 commons common NOUN ct-9406 3 15 attribution attribution NOUN ct-9406 3 16 - - PUNCT ct-9406 3 17 noncommercial noncommercial ADJ ct-9406 3 18 4.0 4.0 NUM ct-9406 3 19 international international ADJ ct-9406 3 20 license license NOUN ct-9406 3 21 ( ( PUNCT ct-9406 3 22 http:// http:// PROPN ct-9406 3 23 creativecommons.org/licenses/by-nc/4.0/ creativecommons.org/licenses/by-nc/4.0/ NOUN ct-9406 3 24 ) ) PUNCT ct-9406 3 25 , , PUNCT ct-9406 3 26 permitting permit VERB ct-9406 3 27 all all DET ct-9406 3 28 non non ADJ ct-9406 3 29 - - ADJ ct-9406 3 30 commercial commercial ADJ ct-9406 3 31 use use NOUN ct-9406 3 32 , , PUNCT ct-9406 3 33 distribution distribution NOUN ct-9406 3 34 , , PUNCT ct-9406 3 35 and and CCONJ ct-9406 3 36 reproduction reproduction NOUN ct-9406 3 37 in in ADP ct-9406 3 38 any any DET ct-9406 3 39 medium medium NOUN ct-9406 3 40 , , PUNCT ct-9406 3 41 provided provide VERB ct-9406 3 42 the the DET ct-9406 3 43 original original ADJ ct-9406 3 44 work work NOUN ct-9406 3 45 is be AUX ct-9406 3 46 properly properly ADV ct-9406 3 47 cited cite VERB ct-9406 3 48 . . PUNCT ct-9406 4 1 citation citation NOUN ct-9406 4 2 line line NOUN ct-9406 4 3 : : PUNCT ct-9406 4 4 clinical clinical ADJ ct-9406 4 5 theriogenology theriogenology NOUN ct-9406 4 6 2023 2023 NUM ct-9406 4 7 , , PUNCT ct-9406 4 8 15 15 NUM ct-9406 4 9 , , PUNCT ct-9406 4 10 9406 9406 NUM ct-9406 4 11 , , PUNCT ct-9406 4 12 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 VERB ct-9406 4 13 review review NOUN ct-9406 4 14 report report NOUN ct-9406 4 15 ( ( PUNCT ct-9406 4 16 invited invite VERB ct-9406 4 17 ) ) PUNCT ct-9406 4 18 clinical clinical ADJ ct-9406 4 19 applications application NOUN ct-9406 4 20 of of ADP ct-9406 4 21 flow flow NOUN ct-9406 4 22 cytometric cytometric ADJ ct-9406 4 23 sperm sperm NOUN ct-9406 4 24 analyses analysis NOUN ct-9406 4 25 fernando fernando PROPN ct-9406 4 26 peña peña PROPN ct-9406 4 27 , , PUNCT ct-9406 4 28 a a DET ct-9406 4 29 heriberto heriberto PROPN ct-9406 4 30 rodriguez rodriguez NOUN ct-9406 4 31 - - PUNCT ct-9406 4 32 martinezb martinezb PROPN ct-9406 4 33 alaboratory alaboratory NOUN ct-9406 4 34 of of ADP ct-9406 4 35 equine equine NOUN ct-9406 4 36 reproduction reproduction NOUN ct-9406 4 37 and and CCONJ ct-9406 4 38 equine equine NOUN ct-9406 4 39 spermatology spermatology NOUN ct-9406 4 40 , , PUNCT ct-9406 4 41 veterinary veterinary ADJ ct-9406 4 42 teaching teaching NOUN ct-9406 4 43 hospital hospital NOUN ct-9406 4 44 , , PUNCT ct-9406 4 45 university university NOUN ct-9406 4 46 of of ADP ct-9406 4 47 extremadura extremadura PROPN ct-9406 4 48 , , PUNCT ct-9406 4 49 cáceres cácere NOUN ct-9406 4 50 , , PUNCT ct-9406 4 51 spain spain PROPN ct-9406 4 52 bdepartment bdepartment NOUN ct-9406 4 53 of of ADP ct-9406 4 54 biomedical biomedical ADJ ct-9406 4 55 and and CCONJ ct-9406 4 56 clinical clinical ADJ ct-9406 4 57 sciences science NOUN ct-9406 4 58 , , PUNCT ct-9406 4 59 division division NOUN ct-9406 4 60 of of ADP ct-9406 4 61 obstetrics obstetric NOUN ct-9406 4 62 and and CCONJ ct-9406 4 63 gynaecology gynaecology NOUN ct-9406 4 64 , , PUNCT ct-9406 4 65 linköping linköpe VERB ct-9406 4 66 university university NOUN ct-9406 4 67 , , PUNCT ct-9406 4 68 linköping linköpe VERB ct-9406 4 69 , , PUNCT ct-9406 4 70 sweden sweden PROPN ct-9406 4 71 abstract abstract ADJ ct-9406 4 72 animal animal NOUN ct-9406 4 73 andrology andrology NOUN ct-9406 4 74 has have AUX ct-9406 4 75 benefitted benefit VERB ct-9406 4 76 by by ADP ct-9406 4 77 flow flow NOUN ct-9406 4 78 cytometry cytometry NOUN ct-9406 4 79 use use NOUN ct-9406 4 80 ( ( PUNCT ct-9406 4 81 to to PART ct-9406 4 82 analyze analyze VERB ct-9406 4 83 various various ADJ ct-9406 4 84 sperm sperm NOUN ct-9406 4 85 components component NOUN ct-9406 4 86 ) ) PUNCT ct-9406 4 87 . . PUNCT ct-9406 5 1 examples example NOUN ct-9406 5 2 include include VERB ct-9406 5 3 its its PRON ct-9406 5 4 use use NOUN ct-9406 5 5 to to PART ct-9406 5 6 explore explore VERB ct-9406 5 7 dna dna PROPN ct-9406 5 8 intactness intactness NOUN ct-9406 5 9 , , PUNCT ct-9406 5 10 to to PART ct-9406 5 11 sort sort VERB ct-9406 5 12 sperm sperm NOUN ct-9406 5 13 for for ADP ct-9406 5 14 chromosomal chromosomal ADJ ct-9406 5 15 sex sex NOUN ct-9406 5 16 , , PUNCT ct-9406 5 17 or or CCONJ ct-9406 5 18 to to PART ct-9406 5 19 explore explore VERB ct-9406 5 20 via via ADP ct-9406 5 21 suitable suitable ADJ ct-9406 5 22 fluorophores fluorophore NOUN ct-9406 5 23 the the DET ct-9406 5 24 functional functional ADJ ct-9406 5 25 intactness intactness NOUN ct-9406 5 26 of of ADP ct-9406 5 27 essential essential ADJ ct-9406 5 28 attributes attribute NOUN ct-9406 5 29 ( ( PUNCT ct-9406 5 30 e.g. e.g. ADV ct-9406 5 31 plasma plasma NOUN ct-9406 5 32 membrane membrane NOUN ct-9406 5 33 ) ) PUNCT ct-9406 5 34 . . PUNCT ct-9406 6 1 these these DET ct-9406 6 2 explorations exploration NOUN ct-9406 6 3 can can AUX ct-9406 6 4 provide provide VERB ct-9406 6 5 the the DET ct-9406 6 6 clinician clinician NOUN ct-9406 6 7 with with ADP ct-9406 6 8 evidence evidence NOUN ct-9406 6 9 of of ADP ct-9406 6 10 the the DET ct-9406 6 11 extent extent NOUN ct-9406 6 12 of of ADP ct-9406 6 13 cell cell NOUN ct-9406 6 14 death death NOUN ct-9406 6 15 , , PUNCT ct-9406 6 16 a a DET ct-9406 6 17 major major ADJ ct-9406 6 18 variable variable NOUN ct-9406 6 19 in in ADP ct-9406 6 20 using use VERB ct-9406 6 21 semen semen NOUN ct-9406 6 22 for for ADP ct-9406 6 23 breeding breed VERB ct-9406 6 24 . . PUNCT ct-9406 7 1 such such ADJ ct-9406 7 2 gains gain NOUN ct-9406 7 3 have have AUX ct-9406 7 4 evolved evolve VERB ct-9406 7 5 over over ADP ct-9406 7 6 the the DET ct-9406 7 7 past past ADJ ct-9406 7 8 decade(s decade(s NOUN ct-9406 7 9 ) ) PUNCT ct-9406 7 10 through through ADP ct-9406 7 11 the the DET ct-9406 7 12 production production NOUN ct-9406 7 13 of of ADP ct-9406 7 14 versatile versatile ADJ ct-9406 7 15 markers marker NOUN ct-9406 7 16 , , PUNCT ct-9406 7 17 including include VERB ct-9406 7 18 biomarkers biomarker NOUN ct-9406 7 19 , , PUNCT ct-9406 7 20 the the DET ct-9406 7 21 design design NOUN ct-9406 7 22 of of ADP ct-9406 7 23 simpler simple ADJ ct-9406 7 24 protocols protocol NOUN ct-9406 7 25 , , PUNCT ct-9406 7 26 and and CCONJ ct-9406 7 27 the the DET ct-9406 7 28 availability availability NOUN ct-9406 7 29 of of ADP ct-9406 7 30 more more ADJ ct-9406 7 31 user user NOUN ct-9406 7 32 - - PUNCT ct-9406 7 33 friendly friendly ADJ ct-9406 7 34 and and CCONJ ct-9406 7 35 less less ADV ct-9406 7 36 expensive expensive ADJ ct-9406 7 37 benchflow benchflow NOUN ct-9406 7 38 cytometers cytometer NOUN ct-9406 7 39 . . PUNCT ct-9406 8 1 the the DET ct-9406 8 2 present present ADJ ct-9406 8 3 review review NOUN ct-9406 8 4 summarizes summarize VERB ct-9406 8 5 the the DET ct-9406 8 6 current current ADJ ct-9406 8 7 state state NOUN ct-9406 8 8 of of ADP ct-9406 8 9 flow flow NOUN ct-9406 8 10 cytometry cytometry NOUN ct-9406 8 11 use use NOUN ct-9406 8 12 within within ADP ct-9406 8 13 animal animal NOUN ct-9406 8 14 andrology andrology NOUN ct-9406 8 15 . . PUNCT ct-9406 9 1 it it PRON ct-9406 9 2 attempts attempt VERB ct-9406 9 3 to to PART ct-9406 9 4 be be AUX ct-9406 9 5 critical critical ADJ ct-9406 9 6 of of ADP ct-9406 9 7 research research NOUN ct-9406 9 8 gains gain NOUN ct-9406 9 9 , , PUNCT ct-9406 9 10 separating separate VERB ct-9406 9 11 them they PRON ct-9406 9 12 for for ADP ct-9406 9 13 clinical clinical ADJ ct-9406 9 14 relevance relevance NOUN ct-9406 9 15 and and CCONJ ct-9406 9 16 focusing focus VERB ct-9406 9 17 on on ADP ct-9406 9 18 its its PRON ct-9406 9 19 use use NOUN ct-9406 9 20 to to PART ct-9406 9 21 examine examine VERB ct-9406 9 22 the the DET ct-9406 9 23 early early ADJ ct-9406 9 24 events event NOUN ct-9406 9 25 that that PRON ct-9406 9 26 occur occur VERB ct-9406 9 27 in in ADP ct-9406 9 28 the the DET ct-9406 9 29 labile labile ADJ ct-9406 9 30 sperm sperm NOUN ct-9406 9 31 plasma plasma NOUN ct-9406 9 32 membrane membrane NOUN ct-9406 9 33 ( ( PUNCT ct-9406 9 34 leading lead VERB ct-9406 9 35 to to ADP ct-9406 9 36 cell cell NOUN ct-9406 9 37 death death NOUN ct-9406 9 38 ) ) PUNCT ct-9406 9 39 – – PUNCT ct-9406 9 40 a a DET ct-9406 9 41 most most ADV ct-9406 9 42 relevant relevant ADJ ct-9406 9 43 marker marker NOUN ct-9406 9 44 for for ADP ct-9406 9 45 clinical clinical ADJ ct-9406 9 46 decisions decision NOUN ct-9406 9 47 during during ADP ct-9406 9 48 diagnosis diagnosis NOUN ct-9406 9 49 and and CCONJ ct-9406 9 50 prognosis prognosis NOUN ct-9406 9 51 of of ADP ct-9406 9 52 fertility fertility NOUN ct-9406 9 53 when when SCONJ ct-9406 9 54 using use VERB ct-9406 9 55 ejaculated ejaculated ADJ ct-9406 9 56 sperm sperm NOUN ct-9406 9 57 ( ( PUNCT ct-9406 9 58 either either CCONJ ct-9406 9 59 unprocessed unprocesse VERB ct-9406 9 60 or or CCONJ ct-9406 9 61 processed process VERB ct-9406 9 62 ) ) PUNCT ct-9406 9 63 for for ADP ct-9406 9 64 cooling cool VERB ct-9406 9 65 , , PUNCT ct-9406 9 66 freezing freezing NOUN ct-9406 9 67 , , PUNCT ct-9406 9 68 or or CCONJ ct-9406 9 69 sperm sperm NOUN ct-9406 9 70 sorting sort VERB ct-9406 9 71 for for ADP ct-9406 9 72 in in ADP ct-9406 9 73 vitro vitro X ct-9406 9 74 fertilization fertilization NOUN ct-9406 9 75 or or CCONJ ct-9406 9 76 artificial artificial ADJ ct-9406 9 77 insemination insemination NOUN ct-9406 9 78 . . PUNCT ct-9406 10 1 keywords keyword NOUN ct-9406 10 2 : : PUNCT ct-9406 10 3 semen semen NOUN ct-9406 10 4 analysis analysis NOUN ct-9406 10 5 , , PUNCT ct-9406 10 6 stallion stallion NOUN ct-9406 10 7 , , PUNCT ct-9406 10 8 flow flow NOUN ct-9406 10 9 cytometry cytometry NOUN ct-9406 10 10 , , PUNCT ct-9406 10 11 sperm sperm NOUN ct-9406 10 12 membrane membrane NOUN ct-9406 10 13 , , PUNCT ct-9406 10 14 livestock livestock NOUN ct-9406 10 15 introduction introduction NOUN ct-9406 10 16 clinical clinical ADJ ct-9406 10 17 veterinary veterinary ADJ ct-9406 10 18 andrology andrology NOUN ct-9406 10 19 attempts attempt VERB ct-9406 10 20 to to PART ct-9406 10 21 assess assess VERB ct-9406 10 22 the the DET ct-9406 10 23 health health NOUN ct-9406 10 24 of of ADP ct-9406 10 25 a a DET ct-9406 10 26 breeding breeding NOUN ct-9406 10 27 male male NOUN ct-9406 10 28 . . PUNCT ct-9406 11 1 it it PRON ct-9406 11 2 certainly certainly ADV ct-9406 11 3 includes include VERB ct-9406 11 4 examination examination NOUN ct-9406 11 5 of of ADP ct-9406 11 6 genital genital ADJ ct-9406 11 7 organs organ NOUN ct-9406 11 8 , , PUNCT ct-9406 11 9 the the DET ct-9406 11 10 ability ability NOUN ct-9406 11 11 to to PART ct-9406 11 12 mate mate VERB ct-9406 11 13 , , PUNCT ct-9406 11 14 and and CCONJ ct-9406 11 15 a a DET ct-9406 11 16 thorough thorough ADJ ct-9406 11 17 examination examination NOUN ct-9406 11 18 of of ADP ct-9406 11 19 collected collect VERB ct-9406 11 20 semen semen NOUN ct-9406 11 21 , , PUNCT ct-9406 11 22 pursuing pursue VERB ct-9406 11 23 a a DET ct-9406 11 24 species species NOUN ct-9406 11 25 - - PUNCT ct-9406 11 26 specific specific ADJ ct-9406 11 27 general general ADJ ct-9406 11 28 breeding breeding NOUN ct-9406 11 29 soundness soundness NOUN ct-9406 11 30 evaluation.1 evaluation.1 NOUN ct-9406 11 31 decades decade NOUN ct-9406 11 32 of of ADP ct-9406 11 33 animal animal NOUN ct-9406 11 34 selection selection NOUN ct-9406 11 35 and and CCONJ ct-9406 11 36 dedicated dedicated ADJ ct-9406 11 37 clinical clinical ADJ ct-9406 11 38 work work NOUN ct-9406 11 39 have have AUX ct-9406 11 40 yielded yield VERB ct-9406 11 41 substantial substantial ADJ ct-9406 11 42 knowledge knowledge NOUN ct-9406 11 43 for for ADP ct-9406 11 44 early early ADJ ct-9406 11 45 identification identification NOUN ct-9406 11 46 of of ADP ct-9406 11 47 potentially potentially ADV ct-9406 11 48 infertile infertile ADJ ct-9406 11 49 sires sire NOUN ct-9406 11 50 , , PUNCT ct-9406 11 51 preventing prevent VERB ct-9406 11 52 their their PRON ct-9406 11 53 use use NOUN ct-9406 11 54 for for ADP ct-9406 11 55 the the DET ct-9406 11 56 widespread widespread ADJ ct-9406 11 57 and and CCONJ ct-9406 11 58 most most ADV ct-9406 11 59 successful successful ADJ ct-9406 11 60 reproductive reproductive ADJ ct-9406 11 61 biotechnology biotechnology NOUN ct-9406 11 62 , , PUNCT ct-9406 11 63 artificial artificial ADJ ct-9406 11 64 insemination insemination NOUN ct-9406 11 65 ( ( PUNCT ct-9406 11 66 ai ai NOUN ct-9406 11 67 ) ) PUNCT ct-9406 11 68 . . PUNCT ct-9406 12 1 furthermore furthermore ADV ct-9406 12 2 , , PUNCT ct-9406 12 3 laboratory laboratory NOUN ct-9406 12 4 examination examination NOUN ct-9406 12 5 of of ADP ct-9406 12 6 semen semen NOUN ct-9406 12 7 has have AUX ct-9406 12 8 provided provide VERB ct-9406 12 9 steady steady ADJ ct-9406 12 10 support support NOUN ct-9406 12 11 for for ADP ct-9406 12 12 accurate accurate ADJ ct-9406 12 13 diagnoses diagnosis NOUN ct-9406 12 14 , , PUNCT ct-9406 12 15 particularly particularly ADV ct-9406 12 16 when when SCONJ ct-9406 12 17 novel novel ADJ ct-9406 12 18 methods method NOUN ct-9406 12 19 and and CCONJ ct-9406 12 20 equipment equipment NOUN ct-9406 12 21 have have AUX ct-9406 12 22 been be AUX ct-9406 12 23 presented.2 presented.2 NOUN ct-9406 12 24 despite despite SCONJ ct-9406 12 25 methods method NOUN ct-9406 12 26 for for ADP ct-9406 12 27 assessing assess VERB ct-9406 12 28 semen semen NOUN ct-9406 12 29 normality normality NOUN ct-9406 12 30 and and CCONJ ct-9406 12 31 , , PUNCT ct-9406 12 32 particularly particularly ADV ct-9406 12 33 , , PUNCT ct-9406 12 34 sperm sperm NOUN ct-9406 12 35 structure structure NOUN ct-9406 12 36 and and CCONJ ct-9406 12 37 function function NOUN ct-9406 12 38 have have AUX ct-9406 12 39 been be AUX ct-9406 12 40 a a DET ct-9406 12 41 target target NOUN ct-9406 12 42 for for ADP ct-9406 12 43 andrological andrological ADJ ct-9406 12 44 and and CCONJ ct-9406 12 45 spermatological spermatological ADJ ct-9406 12 46 research research NOUN ct-9406 12 47 , , PUNCT ct-9406 12 48 the the DET ct-9406 12 49 conventional conventional ADJ ct-9406 12 50 evaluation evaluation NOUN ct-9406 12 51 of of ADP ct-9406 12 52 the the DET ct-9406 12 53 ejaculate ejaculate NOUN ct-9406 12 54 made make VERB ct-9406 12 55 by by ADP ct-9406 12 56 most most ADJ ct-9406 12 57 clinicians clinician NOUN ct-9406 12 58 has have AUX ct-9406 12 59 been be AUX ct-9406 12 60 restricted restrict VERB ct-9406 12 61 to to ADP ct-9406 12 62 determinations determination NOUN ct-9406 12 63 of of ADP ct-9406 12 64 sperm sperm NOUN ct-9406 12 65 number number NOUN ct-9406 12 66 , , PUNCT ct-9406 12 67 motility motility NOUN ct-9406 12 68 , , PUNCT ct-9406 12 69 and and CCONJ ct-9406 12 70 very very ADV ct-9406 12 71 seldom seldom ADV ct-9406 12 72 , , PUNCT ct-9406 12 73 morphology.3–5 morphology.3–5 NOUN ct-9406 12 74 this this DET ct-9406 12 75 slim slim ADJ ct-9406 12 76 battery battery NOUN ct-9406 12 77 of of ADP ct-9406 12 78 semen semen NOUN ct-9406 12 79 parameters parameter NOUN ct-9406 12 80 has have AUX ct-9406 12 81 historically historically ADV ct-9406 12 82 been be AUX ct-9406 12 83 considered consider VERB ct-9406 12 84 sufficient sufficient ADJ ct-9406 12 85 to to PART ct-9406 12 86 potentially potentially ADV ct-9406 12 87 separate separate VERB ct-9406 12 88 fertile fertile ADV ct-9406 12 89 from from ADP ct-9406 12 90 infertile.6 infertile.6 PROPN ct-9406 12 91 yet yet ADV ct-9406 12 92 , , PUNCT ct-9406 12 93 the the DET ct-9406 12 94 proportion proportion NOUN ct-9406 12 95 of of ADP ct-9406 12 96 subfertile subfertile ADJ ct-9406 12 97 sires sire NOUN ct-9406 12 98 is be AUX ct-9406 12 99 still still ADV ct-9406 12 100 sufficiently sufficiently ADV ct-9406 12 101 high high ADJ ct-9406 12 102 to to PART ct-9406 12 103 cause cause VERB ct-9406 12 104 losses loss NOUN ct-9406 12 105 on on ADP ct-9406 12 106 the the DET ct-9406 12 107 level level NOUN ct-9406 12 108 of of ADP ct-9406 12 109 billions billion NOUN ct-9406 12 110 of of ADP ct-9406 12 111 euros euro NOUN ct-9406 12 112 or or CCONJ ct-9406 12 113 dollars dollar NOUN ct-9406 12 114 , , PUNCT ct-9406 12 115 yearly yearly ADV ct-9406 12 116 . . PUNCT ct-9406 13 1 these these DET ct-9406 13 2 losses loss NOUN ct-9406 13 3 are be AUX ct-9406 13 4 often often ADV ct-9406 13 5 recognized recognize VERB ct-9406 13 6 too too ADV ct-9406 13 7 late late ADV ct-9406 13 8 , , PUNCT ct-9406 13 9 either either CCONJ ct-9406 13 10 when when SCONJ ct-9406 13 11 conception conception NOUN ct-9406 13 12 is be AUX ct-9406 13 13 clinically clinically ADV ct-9406 13 14 assessed assess VERB ct-9406 13 15 , , PUNCT ct-9406 13 16 when when SCONJ ct-9406 13 17 nonreturn nonreturn NOUN ct-9406 13 18 figures figure NOUN ct-9406 13 19 are be AUX ct-9406 13 20 indirectly indirectly ADV ct-9406 13 21 measured measure VERB ct-9406 13 22 , , PUNCT ct-9406 13 23 or or CCONJ ct-9406 13 24 when when SCONJ ct-9406 13 25 offspring offspring NOUN ct-9406 13 26 is be AUX ct-9406 13 27 absent absent ADJ ct-9406 13 28 or or CCONJ ct-9406 13 29 lower low ADJ ct-9406 13 30 than than SCONJ ct-9406 13 31 expected expect VERB ct-9406 13 32 . . PUNCT ct-9406 14 1 although although SCONJ ct-9406 14 2 sire sire NOUN ct-9406 14 3 fertility fertility NOUN ct-9406 14 4 is be AUX ct-9406 14 5 evidently evidently ADV ct-9406 14 6 a a DET ct-9406 14 7 limiting limit VERB ct-9406 14 8 factor factor NOUN ct-9406 14 9 , , PUNCT ct-9406 14 10 most most ADJ ct-9406 14 11 fertility fertility NOUN ct-9406 14 12 research research NOUN ct-9406 14 13 seldom seldom ADV ct-9406 14 14 focuses focus VERB ct-9406 14 15 on on ADP ct-9406 14 16 the the DET ct-9406 14 17 male male NOUN ct-9406 14 18 or or CCONJ ct-9406 14 19 the the DET ct-9406 14 20 quality quality NOUN ct-9406 14 21 of of ADP ct-9406 14 22 the the DET ct-9406 14 23 used used ADJ ct-9406 14 24 sperm.6 sperm.6 NOUN ct-9406 14 25 one one NUM ct-9406 14 26 of of ADP ct-9406 14 27 the the DET ct-9406 14 28 reasons reason NOUN ct-9406 14 29 behind behind ADV ct-9406 14 30 is be AUX ct-9406 14 31 that that SCONJ ct-9406 14 32 although although SCONJ ct-9406 14 33 sperm sperm NOUN ct-9406 14 34 biomarkers biomarker NOUN ct-9406 14 35 related relate VERB ct-9406 14 36 to to ADP ct-9406 14 37 fertility fertility NOUN ct-9406 14 38 exist exist VERB ct-9406 14 39 , , PUNCT ct-9406 14 40 their their PRON ct-9406 14 41 use use NOUN ct-9406 14 42 by by ADP ct-9406 14 43 the the DET ct-9406 14 44 clinician clinician NOUN ct-9406 14 45 is be AUX ct-9406 14 46 still still ADV ct-9406 14 47 burdened burden VERB ct-9406 14 48 by by ADP ct-9406 14 49 requirements requirement NOUN ct-9406 14 50 of of ADP ct-9406 14 51 high high ADJ ct-9406 14 52 technical technical ADJ ct-9406 14 53 know know NOUN ct-9406 14 54 - - PUNCT ct-9406 14 55 how how NOUN ct-9406 14 56 , , PUNCT ct-9406 14 57 expensive expensive ADJ ct-9406 14 58 instrumentation instrumentation NOUN ct-9406 14 59 , , PUNCT ct-9406 14 60 and and CCONJ ct-9406 14 61 the the DET ct-9406 14 62 inability inability NOUN ct-9406 14 63 of of ADP ct-9406 14 64 the the DET ct-9406 14 65 researchers researcher NOUN ct-9406 14 66 to to PART ct-9406 14 67 communicate communicate VERB ct-9406 14 68 their their PRON ct-9406 14 69 diagnostic diagnostic ADJ ct-9406 14 70 value value NOUN ct-9406 14 71 , , PUNCT ct-9406 14 72 mostly mostly ADV ct-9406 14 73 due due ADJ ct-9406 14 74 to to ADP ct-9406 14 75 insufficient insufficient ADJ ct-9406 14 76 standardization standardization NOUN ct-9406 14 77 of of ADP ct-9406 14 78 valuable valuable ADJ ct-9406 14 79 protocols protocol NOUN ct-9406 14 80 since since SCONJ ct-9406 14 81 the the DET ct-9406 14 82 use use NOUN ct-9406 14 83 flow flow NOUN ct-9406 14 84 cytometry cytometry NOUN ct-9406 14 85 is be AUX ct-9406 14 86 becoming become VERB ct-9406 14 87 common common ADJ ct-9406 14 88 in in ADP ct-9406 14 89 established establish VERB ct-9406 14 90 companies.6 companies.6 NOUN ct-9406 14 91 another another DET ct-9406 14 92 factor factor NOUN ct-9406 14 93 to to PART ct-9406 14 94 consider consider VERB ct-9406 14 95 is be AUX ct-9406 14 96 the the DET ct-9406 14 97 complex complex ADJ ct-9406 14 98 nature nature NOUN ct-9406 14 99 of of ADP ct-9406 14 100 fertility fertility NOUN ct-9406 14 101 depending depend VERB ct-9406 14 102 on on ADP ct-9406 14 103 numerous numerous ADJ ct-9406 14 104 factors factor NOUN ct-9406 14 105 and and CCONJ ct-9406 14 106 their their PRON ct-9406 14 107 interactions.2,6,7 interactions.2,6,7 ADJ ct-9406 14 108 innumerable innumerable ADJ ct-9406 14 109 methods method NOUN ct-9406 14 110 are be AUX ct-9406 14 111 now now ADV ct-9406 14 112 available available ADJ ct-9406 14 113 for for ADP ct-9406 14 114 semen semen NOUN ct-9406 14 115 / / SYM ct-9406 14 116 sperm sperm NOUN ct-9406 14 117 evaluation evaluation NOUN ct-9406 14 118 , , PUNCT ct-9406 14 119 including include VERB ct-9406 14 120 those those PRON ct-9406 14 121 examining examine VERB ct-9406 14 122 the the DET ct-9406 14 123 capability capability NOUN ct-9406 14 124 of of ADP ct-9406 14 125 sperm sperm NOUN ct-9406 14 126 mailto:heriberto.rodriguez-martinez@liu.se mailto:heriberto.rodriguez-martinez@liu.se NOUN ct-9406 14 127 http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ PROPN ct-9406 14 128 http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ PROPN ct-9406 14 129 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 VERB ct-9406 14 130 2 2 NUM ct-9406 14 131 citation citation NOUN ct-9406 14 132 line line NOUN ct-9406 14 133 : : PUNCT ct-9406 14 134 clinical clinical ADJ ct-9406 14 135 theriogenology theriogenology NOUN ct-9406 14 136 2023 2023 NUM ct-9406 14 137 , , PUNCT ct-9406 14 138 15 15 NUM ct-9406 14 139 , , PUNCT ct-9406 14 140 9406 9406 NUM ct-9406 14 141 , , PUNCT ct-9406 14 142 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 NOUN ct-9406 14 143 to to PART ct-9406 14 144 interact interact VERB ct-9406 14 145 with with ADP ct-9406 14 146 the the DET ct-9406 14 147 surrounding surround VERB ct-9406 14 148 fluids fluid NOUN ct-9406 14 149 ( ( PUNCT ct-9406 14 150 seminal seminal ADJ ct-9406 14 151 plasma plasma NOUN ct-9406 14 152 , , PUNCT ct-9406 14 153 intralumenal intralumenal ADJ ct-9406 14 154 female female ADJ ct-9406 14 155 genital genital ADJ ct-9406 14 156 fluids fluid NOUN ct-9406 14 157 and and CCONJ ct-9406 14 158 extension extension NOUN ct-9406 14 159 artificial artificial ADJ ct-9406 14 160 media medium NOUN ct-9406 14 161 we we PRON ct-9406 14 162 use use VERB ct-9406 14 163 for for ADP ct-9406 14 164 preservation preservation NOUN ct-9406 14 165 , , PUNCT ct-9406 14 166 in in ADP ct-9406 14 167 vitro vitro X ct-9406 14 168 culture culture NOUN ct-9406 14 169 , , PUNCT ct-9406 14 170 and and CCONJ ct-9406 14 171 sorting sorting NOUN ct-9406 14 172 ) ) PUNCT ct-9406 14 173 , , PUNCT ct-9406 14 174 the the DET ct-9406 14 175 lining line VERB ct-9406 14 176 epithelia epithelia NOUN ct-9406 14 177 of of ADP ct-9406 14 178 the the DET ct-9406 14 179 internal internal ADJ ct-9406 14 180 female female ADJ ct-9406 14 181 tract tract NOUN ct-9406 14 182 , , PUNCT ct-9406 14 183 or or CCONJ ct-9406 14 184 the the DET ct-9406 14 185 coatings coating NOUN ct-9406 14 186 of of ADP ct-9406 14 187 the the DET ct-9406 14 188 oocyte.8–11 oocyte.8–11 NOUN ct-9406 14 189 currently currently ADV ct-9406 14 190 , , PUNCT ct-9406 14 191 we we PRON ct-9406 14 192 can can AUX ct-9406 14 193 explore explore VERB ct-9406 14 194 not not PART ct-9406 14 195 only only ADV ct-9406 14 196 sperm sperm NOUN ct-9406 14 197 plasma plasma NOUN ct-9406 14 198 membrane membrane NOUN ct-9406 14 199 status status NOUN ct-9406 14 200 , , PUNCT ct-9406 14 201 various various ADJ ct-9406 14 202 sperm sperm NOUN ct-9406 14 203 organelles organelle NOUN ct-9406 14 204 , , PUNCT ct-9406 14 205 sperm sperm NOUN ct-9406 14 206 genome genome NOUN ct-9406 14 207 , , PUNCT ct-9406 14 208 and and CCONJ ct-9406 14 209 its its PRON ct-9406 14 210 transcriptome transcriptome NOUN ct-9406 14 211 but but CCONJ ct-9406 14 212 also also ADV ct-9406 14 213 the the DET ct-9406 14 214 capacity capacity NOUN ct-9406 14 215 of of ADP ct-9406 14 216 sperm sperm NOUN ct-9406 14 217 to to PART ct-9406 14 218 fertilize fertilize VERB ct-9406 14 219 and and CCONJ ct-9406 14 220 to to PART ct-9406 14 221 initiate initiate VERB ct-9406 14 222 early early ADJ ct-9406 14 223 embryo embryo NOUN ct-9406 14 224 development development NOUN ct-9406 14 225 in in ADP ct-9406 14 226 vitro.12–17 vitro.12–17 NOUN ct-9406 14 227 in in ADP ct-9406 14 228 addition addition NOUN ct-9406 14 229 , , PUNCT ct-9406 14 230 we we PRON ct-9406 14 231 are be AUX ct-9406 14 232 helped help VERB ct-9406 14 233 by by ADP ct-9406 14 234 several several ADJ ct-9406 14 235 computer computer NOUN ct-9406 14 236 - - PUNCT ct-9406 14 237 assisted assist VERB ct-9406 14 238 motility motility NOUN ct-9406 14 239 analyzers analyzer NOUN ct-9406 14 240 , , PUNCT ct-9406 14 241 of of ADP ct-9406 14 242 morphology morphology NOUN ct-9406 14 243 screeners screener NOUN ct-9406 14 244 , , PUNCT ct-9406 14 245 and and CCONJ ct-9406 14 246 by by ADP ct-9406 14 247 flow flow NOUN ct-9406 14 248 cytometers cytometer NOUN ct-9406 14 249 of of ADP ct-9406 14 250 various various ADJ ct-9406 14 251 complexity complexity NOUN ct-9406 14 252 ( ( PUNCT ct-9406 14 253 and and CCONJ ct-9406 14 254 cost cost NOUN ct-9406 14 255 ) ) PUNCT ct-9406 14 256 , , PUNCT ct-9406 14 257 including include VERB ct-9406 14 258 cutting cut VERB ct-9406 14 259 - - PUNCT ct-9406 14 260 edge edge NOUN ct-9406 14 261 imagebased imagebase VERB ct-9406 14 262 fluorescence fluorescence NOUN ct-9406 14 263 correlation correlation NOUN ct-9406 14 264 spectroscopy spectroscopy NOUN ct-9406 14 265 for for ADP ct-9406 14 266 high high ADJ ct-9406 14 267 - - PUNCT ct-9406 14 268 throughput throughput NOUN ct-9406 14 269 cell cell NOUN ct-9406 14 270 image image NOUN ct-9406 15 1 acquisition.6,18–20 acquisition.6,18–20 NOUN ct-9406 15 2 authors author NOUN ct-9406 15 3 advise advise VERB ct-9406 15 4 the the DET ct-9406 15 5 readers reader NOUN ct-9406 15 6 to to PART ct-9406 15 7 be be AUX ct-9406 15 8 acquainted acquaint VERB ct-9406 15 9 with with ADP ct-9406 15 10 some some PRON ct-9406 15 11 of of ADP ct-9406 15 12 the the DET ct-9406 15 13 excellent excellent ADJ ct-9406 15 14 reviews review NOUN ct-9406 15 15 available available ADJ ct-9406 15 16 to to PART ct-9406 15 17 grasp grasp VERB ct-9406 15 18 this this DET ct-9406 15 19 development.20,21 development.20,21 NOUN ct-9406 15 20 for for ADP ct-9406 15 21 the the DET ct-9406 15 22 clinician clinician NOUN ct-9406 15 23 , , PUNCT ct-9406 15 24 perhaps perhaps ADV ct-9406 15 25 the the DET ct-9406 15 26 most most ADV ct-9406 15 27 relevant relevant ADJ ct-9406 15 28 take take VERB ct-9406 15 29 - - PUNCT ct-9406 15 30 home home NOUN ct-9406 15 31 message message NOUN ct-9406 15 32 is be AUX ct-9406 15 33 to to PART ct-9406 15 34 remember remember VERB ct-9406 15 35 that that SCONJ ct-9406 15 36 sperm sperm NOUN ct-9406 15 37 are be AUX ct-9406 15 38 highly highly ADV ct-9406 15 39 differentiated differentiated ADJ ct-9406 15 40 cells cell NOUN ct-9406 15 41 , , PUNCT ct-9406 15 42 programmed program VERB ct-9406 15 43 to to PART ct-9406 15 44 interact interact VERB ct-9406 15 45 and and CCONJ ct-9406 15 46 react react VERB ct-9406 15 47 to to ADP ct-9406 15 48 the the DET ct-9406 15 49 environment environment NOUN ct-9406 15 50 and and CCONJ ct-9406 15 51 prompt prompt VERB ct-9406 15 52 to to PART ct-9406 15 53 suffer suffer VERB ct-9406 15 54 changes change NOUN ct-9406 15 55 that that PRON ct-9406 15 56 inexorably inexorably ADV ct-9406 15 57 lead lead VERB ct-9406 15 58 to to ADP ct-9406 15 59 sperm sperm NOUN ct-9406 15 60 death death NOUN ct-9406 15 61 , , PUNCT ct-9406 15 62 and and CCONJ ct-9406 15 63 hence hence ADV ct-9406 15 64 inability inability NOUN ct-9406 15 65 to to PART ct-9406 15 66 participate participate VERB ct-9406 15 67 in in ADP ct-9406 15 68 fertilization fertilization NOUN ct-9406 15 69 , , PUNCT ct-9406 15 70 and and CCONJ ct-9406 15 71 perhaps perhaps ADV ct-9406 15 72 , , PUNCT ct-9406 15 73 to to PART ct-9406 15 74 cause cause VERB ct-9406 15 75 deleterious deleterious ADJ ct-9406 15 76 changes change NOUN ct-9406 15 77 in in ADP ct-9406 15 78 the the DET ct-9406 15 79 accompanying accompany VERB ct-9406 15 80 sperm.22 sperm.22 NOUN ct-9406 15 81 our our PRON ct-9406 15 82 intention intention NOUN ct-9406 15 83 with with ADP ct-9406 15 84 this this DET ct-9406 15 85 review review NOUN ct-9406 15 86 is be AUX ct-9406 15 87 to to PART ct-9406 15 88 remind remind VERB ct-9406 15 89 the the DET ct-9406 15 90 clinician clinician NOUN ct-9406 15 91 of of ADP ct-9406 15 92 the the DET ct-9406 15 93 basic basic ADJ ct-9406 15 94 and and CCONJ ct-9406 15 95 essential essential ADJ ct-9406 15 96 diagnostic diagnostic ADJ ct-9406 15 97 values value NOUN ct-9406 15 98 of of ADP ct-9406 15 99 determining determine VERB ct-9406 15 100 the the DET ct-9406 15 101 percentages percentage NOUN ct-9406 15 102 of of ADP ct-9406 15 103 live live ADJ ct-9406 15 104 sperm sperm NOUN ct-9406 15 105 in in ADP ct-9406 15 106 the the DET ct-9406 15 107 sample sample NOUN ct-9406 15 108 for for ADP ct-9406 15 109 its its PRON ct-9406 15 110 fate fate NOUN ct-9406 15 111 . . PUNCT ct-9406 16 1 this this DET ct-9406 16 2 concept concept NOUN ct-9406 16 3 that that PRON ct-9406 16 4 calls call VERB ct-9406 16 5 for for ADP ct-9406 16 6 methods method NOUN ct-9406 16 7 that that PRON ct-9406 16 8 can can AUX ct-9406 16 9 identify identify VERB ct-9406 16 10 sperm sperm NOUN ct-9406 16 11 changes change NOUN ct-9406 16 12 before before SCONJ ct-9406 16 13 death death NOUN ct-9406 16 14 is be AUX ct-9406 16 15 established establish VERB ct-9406 16 16 , , PUNCT ct-9406 16 17 considering consider VERB ct-9406 16 18 sperm sperm NOUN ct-9406 16 19 death death NOUN ct-9406 16 20 is be AUX ct-9406 16 21 irreversible irreversible ADJ ct-9406 16 22 . . PUNCT ct-9406 17 1 although although SCONJ ct-9406 17 2 the the DET ct-9406 17 3 integrity integrity NOUN ct-9406 17 4 of of ADP ct-9406 17 5 the the DET ct-9406 17 6 plasma plasma NOUN ct-9406 17 7 membrane membrane NOUN ct-9406 17 8 is be AUX ct-9406 17 9 rapidly rapidly ADV ct-9406 17 10 lost lose VERB ct-9406 17 11 when when SCONJ ct-9406 17 12 necrotic necrotic ADJ ct-9406 17 13 cell cell NOUN ct-9406 17 14 death death NOUN ct-9406 17 15 occurs occur VERB ct-9406 17 16 , , PUNCT ct-9406 17 17 subtle subtle ADJ ct-9406 17 18 changes change NOUN ct-9406 17 19 characterize characterize VERB ct-9406 17 20 other other ADJ ct-9406 17 21 forms form NOUN ct-9406 17 22 of of ADP ct-9406 17 23 sperm sperm NOUN ct-9406 17 24 demise demise NOUN ct-9406 17 25 . . PUNCT ct-9406 18 1 numerous numerous ADJ ct-9406 18 2 flow flow NOUN ct-9406 18 3 cytometry cytometry NOUN ct-9406 18 4 ( ( PUNCT ct-9406 18 5 fc fc INTJ ct-9406 18 6 ) ) PUNCT ct-9406 18 7 protocols protocol NOUN ct-9406 18 8 have have AUX ct-9406 18 9 been be AUX ct-9406 18 10 developed develop VERB ct-9406 18 11 to to PART ct-9406 18 12 identify identify VERB ct-9406 18 13 necrotic necrotic ADJ ct-9406 18 14 sperm sperm NOUN ct-9406 18 15 and and CCONJ ct-9406 18 16 also also ADV ct-9406 18 17 various various ADJ ct-9406 18 18 degrees degree NOUN ct-9406 18 19 of of ADP ct-9406 18 20 plasma plasma NOUN ct-9406 18 21 membrane membrane NOUN ct-9406 18 22 alteration alteration NOUN ct-9406 18 23 occurring occur VERB ct-9406 18 24 prior prior ADV ct-9406 18 25 to to ADP ct-9406 18 26 sperm sperm NOUN ct-9406 18 27 death.23–27 death.23–27 NOUN ct-9406 18 28 in in ADP ct-9406 18 29 this this DET ct-9406 18 30 paper paper NOUN ct-9406 18 31 , , PUNCT ct-9406 18 32 we we PRON ct-9406 18 33 provide provide VERB ct-9406 18 34 an an DET ct-9406 18 35 update update NOUN ct-9406 18 36 on on ADP ct-9406 18 37 the the DET ct-9406 18 38 various various ADJ ct-9406 18 39 forms form NOUN ct-9406 18 40 of of ADP ct-9406 18 41 death death NOUN ct-9406 18 42 in in ADP ct-9406 18 43 sperm sperm NOUN ct-9406 18 44 , , PUNCT ct-9406 18 45 and and CCONJ ct-9406 18 46 how how SCONJ ct-9406 18 47 these these DET ct-9406 18 48 forms form NOUN ct-9406 18 49 are be AUX ct-9406 18 50 reflected reflect VERB ct-9406 18 51 on on ADP ct-9406 18 52 various various ADJ ct-9406 18 53 fc fc PROPN ct-9406 18 54 protocols protocol NOUN ct-9406 18 55 , , PUNCT ct-9406 18 56 establishing establish VERB ct-9406 18 57 a a DET ct-9406 18 58 critical critical ADJ ct-9406 18 59 review review NOUN ct-9406 18 60 of of ADP ct-9406 18 61 the the DET ct-9406 18 62 current current ADJ ct-9406 18 63 methods method NOUN ct-9406 18 64 for for ADP ct-9406 18 65 the the DET ct-9406 18 66 determination determination NOUN ct-9406 18 67 of of ADP ct-9406 18 68 the the DET ct-9406 18 69 integrity integrity NOUN ct-9406 18 70 and and CCONJ ct-9406 18 71 functionality functionality NOUN ct-9406 18 72 of of ADP ct-9406 18 73 sperm sperm NOUN ct-9406 18 74 plasma plasma NOUN ct-9406 18 75 membrane membrane NOUN ct-9406 18 76 . . PUNCT ct-9406 19 1 a a DET ct-9406 19 2 particular particular ADJ ct-9406 19 3 call call NOUN ct-9406 19 4 is be AUX ct-9406 19 5 made make VERB ct-9406 19 6 for for ADP ct-9406 19 7 the the DET ct-9406 19 8 value value NOUN ct-9406 19 9 of of ADP ct-9406 19 10 using use VERB ct-9406 19 11 multiparametric multiparametric PROPN ct-9406 19 12 fc fc PROPN ct-9406 19 13 that that PRON ct-9406 19 14 , , PUNCT ct-9406 19 15 aided aid VERB ct-9406 19 16 by by ADP ct-9406 19 17 computational computational ADJ ct-9406 19 18 analysis analysis NOUN ct-9406 19 19 ( ( PUNCT ct-9406 19 20 cytobank cytobank PROPN ct-9406 19 21 ) ) PUNCT ct-9406 19 22 , , PUNCT ct-9406 19 23 can can AUX ct-9406 19 24 simultaneously simultaneously ADV ct-9406 19 25 identify identify VERB ct-9406 19 26 multiple multiple ADJ ct-9406 19 27 forms form NOUN ct-9406 19 28 of of ADP ct-9406 19 29 sperm sperm NOUN ct-9406 19 30 damage damage NOUN ct-9406 19 31 . . PUNCT ct-9406 20 1 although although SCONJ ct-9406 20 2 these these DET ct-9406 20 3 multiple multiple ADJ ct-9406 20 4 commercial commercial ADJ ct-9406 20 5 bench bench NOUN ct-9406 20 6 - - PUNCT ct-9406 20 7 top top NOUN ct-9406 20 8 fcs fc NOUN ct-9406 20 9 are be AUX ct-9406 20 10 available available ADJ ct-9406 20 11 , , PUNCT ct-9406 20 12 the the DET ct-9406 20 13 authors author NOUN ct-9406 20 14 recommend recommend VERB ct-9406 20 15 the the DET ct-9406 20 16 use use NOUN ct-9406 20 17 of of ADP ct-9406 20 18 modern modern ADJ ct-9406 20 19 equipment equipment NOUN ct-9406 20 20 with with ADP ct-9406 20 21 upgrade upgrade NOUN ct-9406 20 22 possibility possibility NOUN ct-9406 20 23 ( ( PUNCT ct-9406 20 24 e.g. e.g. ADV ct-9406 20 25 activation activation NOUN ct-9406 20 26 of of ADP ct-9406 20 27 new new ADJ ct-9406 20 28 lasers laser NOUN ct-9406 20 29 ) ) PUNCT ct-9406 20 30 , , PUNCT ct-9406 20 31 and and CCONJ ct-9406 20 32 having have VERB ct-9406 20 33 a a DET ct-9406 20 34 friendly friendly ADJ ct-9406 20 35 and and CCONJ ct-9406 20 36 open open ADJ ct-9406 20 37 software software NOUN ct-9406 20 38 . . PUNCT ct-9406 21 1 necrosis necrosis NOUN ct-9406 21 2 / / SYM ct-9406 21 3 apoptosis apoptosis NOUN ct-9406 21 4 / / SYM ct-9406 21 5 ferroptosis ferroptosis NOUN ct-9406 21 6 / / SYM ct-9406 21 7 spermptosis/ spermptosis/ NUM ct-9406 21 8 premature premature ADJ ct-9406 21 9 capacitation capacitation NOUN ct-9406 21 10 although although SCONJ ct-9406 21 11 the the DET ct-9406 21 12 existence existence NOUN ct-9406 21 13 of of ADP ct-9406 21 14 classical classical ADJ ct-9406 21 15 necrosis necrosis NOUN ct-9406 21 16 in in ADP ct-9406 21 17 sperm sperm NOUN ct-9406 21 18 is be AUX ct-9406 21 19 widely widely ADV ct-9406 21 20 accepted accept VERB ct-9406 21 21 , , PUNCT ct-9406 21 22 the the DET ct-9406 21 23 presence presence NOUN ct-9406 21 24 of of ADP ct-9406 21 25 various various ADJ ct-9406 21 26 forms form NOUN ct-9406 21 27 of of ADP ct-9406 21 28 programmed program VERB ct-9406 21 29 cell cell NOUN ct-9406 21 30 death death NOUN ct-9406 21 31 has have AUX ct-9406 21 32 been be AUX ct-9406 21 33 under under ADP ct-9406 21 34 debate debate NOUN ct-9406 21 35 . . PUNCT ct-9406 22 1 the the DET ct-9406 22 2 transcriptionally transcriptionally ADV ct-9406 22 3 silent silent ADJ ct-9406 22 4 nature nature NOUN ct-9406 22 5 of of ADP ct-9406 22 6 sperm sperm NOUN ct-9406 22 7 has have AUX ct-9406 22 8 been be AUX ct-9406 22 9 claimed claim VERB ct-9406 22 10 to to PART ct-9406 22 11 make make VERB ct-9406 22 12 programmed programmed ADJ ct-9406 22 13 forms form NOUN ct-9406 22 14 of of ADP ct-9406 22 15 cell cell NOUN ct-9406 22 16 death death NOUN ct-9406 22 17 unlikely unlikely ADJ ct-9406 22 18 in in ADP ct-9406 22 19 these these DET ct-9406 22 20 cells cell NOUN ct-9406 22 21 ; ; PUNCT ct-9406 22 22 however however ADV ct-9406 22 23 , , PUNCT ct-9406 22 24 currently currently ADV ct-9406 22 25 , , PUNCT ct-9406 22 26 it it PRON ct-9406 22 27 is be AUX ct-9406 22 28 considered consider VERB ct-9406 22 29 that that SCONJ ct-9406 22 30 sperm sperm NOUN ct-9406 22 31 are be AUX ct-9406 22 32 programmed program VERB ct-9406 22 33 for for ADP ct-9406 22 34 cell cell NOUN ct-9406 22 35 death death NOUN ct-9406 22 36 as as ADP ct-9406 22 37 a a DET ct-9406 22 38 measure measure NOUN ct-9406 22 39 to to PART ct-9406 22 40 promote promote VERB ct-9406 22 41 the the DET ct-9406 22 42 silent silent ADJ ct-9406 22 43 removal removal NOUN ct-9406 22 44 of of ADP ct-9406 22 45 redundant redundant ADJ ct-9406 22 46 sperm sperm NOUN ct-9406 22 47 for for ADP ct-9406 22 48 the the DET ct-9406 22 49 female female ADJ ct-9406 22 50 reproductive reproductive NOUN ct-9406 22 51 tract.28 tract.28 NOUN ct-9406 22 52 moreover moreover ADV ct-9406 22 53 , , PUNCT ct-9406 22 54 although although SCONJ ct-9406 22 55 protein protein NOUN ct-9406 22 56 synthesis synthesis NOUN ct-9406 22 57 in in ADP ct-9406 22 58 sperm sperm NOUN ct-9406 22 59 is be AUX ct-9406 22 60 negligible negligible ADJ ct-9406 22 61 , , PUNCT ct-9406 22 62 the the DET ct-9406 22 63 incorporation incorporation NOUN ct-9406 22 64 of of ADP ct-9406 22 65 new new ADJ ct-9406 22 66 proteins protein NOUN ct-9406 22 67 through through ADP ct-9406 22 68 microvesicle microvesicle NOUN ct-9406 22 69 trafficking trafficking NOUN ct-9406 22 70 and and CCONJ ct-9406 22 71 , , PUNCT ct-9406 22 72 especially especially ADV ct-9406 22 73 , , PUNCT ct-9406 22 74 posttranslational posttranslational ADJ ct-9406 22 75 modifications modification NOUN ct-9406 22 76 of of ADP ct-9406 22 77 existing exist VERB ct-9406 22 78 proteins protein NOUN ct-9406 22 79 modulate modulate VERB ct-9406 22 80 sperm sperm NOUN ct-9406 22 81 function function NOUN ct-9406 22 82 . . PUNCT ct-9406 23 1 despite despite SCONJ ct-9406 23 2 all all DET ct-9406 23 3 sperm sperm NOUN ct-9406 23 4 are be AUX ct-9406 23 5 activated activate VERB ct-9406 23 6 through through ADP ct-9406 23 7 cell cell NOUN ct-9406 23 8 death death NOUN ct-9406 23 9 , , PUNCT ct-9406 23 10 this this PRON ct-9406 23 11 may may AUX ct-9406 23 12 occur occur VERB ct-9406 23 13 at at ADP ct-9406 23 14 various various ADJ ct-9406 23 15 velocities velocity NOUN ct-9406 23 16 and and CCONJ ct-9406 23 17 factors factor NOUN ct-9406 23 18 , , PUNCT ct-9406 23 19 i.e. i.e. X ct-9406 23 20 oxidative oxidative ADJ ct-9406 23 21 stress stress NOUN ct-9406 23 22 , , PUNCT ct-9406 23 23 triggering trigger VERB ct-9406 23 24 and and CCONJ ct-9406 23 25 accelerating accelerate VERB ct-9406 23 26 the the DET ct-9406 23 27 process process NOUN ct-9406 23 28 . . PUNCT ct-9406 24 1 although although SCONJ ct-9406 24 2 the the DET ct-9406 24 3 terms term NOUN ct-9406 24 4 programmed program VERB ct-9406 24 5 cell cell NOUN ct-9406 24 6 death death NOUN ct-9406 24 7 and and CCONJ ct-9406 24 8 apoptosis apoptosis NOUN ct-9406 24 9 have have AUX ct-9406 24 10 been be AUX ct-9406 24 11 used use VERB ct-9406 24 12 indistinctly indistinctly ADV ct-9406 24 13 , , PUNCT ct-9406 24 14 these these DET ct-9406 24 15 terms term NOUN ct-9406 24 16 are be AUX ct-9406 24 17 not not PART ct-9406 24 18 synonymous synonymous ADJ ct-9406 24 19 . . PUNCT ct-9406 25 1 various various ADJ ct-9406 25 2 forms form NOUN ct-9406 25 3 of of ADP ct-9406 25 4 programmed program VERB ct-9406 25 5 cell cell NOUN ct-9406 25 6 death death NOUN ct-9406 25 7 may may AUX ct-9406 25 8 share share VERB ct-9406 25 9 similar similar ADJ ct-9406 25 10 molecular molecular ADJ ct-9406 25 11 mechanisms mechanism NOUN ct-9406 25 12 ; ; PUNCT ct-9406 25 13 however however ADV ct-9406 25 14 , , PUNCT ct-9406 25 15 all all DET ct-9406 25 16 these these DET ct-9406 25 17 forms form NOUN ct-9406 25 18 are be AUX ct-9406 25 19 not not PART ct-9406 25 20 identical identical ADJ ct-9406 25 21 . . PUNCT ct-9406 26 1 necrosis necrosis NOUN ct-9406 26 2 is be AUX ct-9406 26 3 characterized characterize VERB ct-9406 26 4 by by ADP ct-9406 26 5 a a DET ct-9406 26 6 rapid rapid ADJ ct-9406 26 7 loss loss NOUN ct-9406 26 8 of of ADP ct-9406 26 9 membrane membrane NOUN ct-9406 26 10 integrity integrity NOUN ct-9406 26 11 . . PUNCT ct-9406 27 1 however however ADV ct-9406 27 2 , , PUNCT ct-9406 27 3 in in ADP ct-9406 27 4 various various ADJ ct-9406 27 5 forms form NOUN ct-9406 27 6 of of ADP ct-9406 27 7 programmed programmed ADJ ct-9406 27 8 cell cell NOUN ct-9406 27 9 death death NOUN ct-9406 27 10 , , PUNCT ct-9406 27 11 more more ADV ct-9406 27 12 subtle subtle ADJ ct-9406 27 13 changes change NOUN ct-9406 27 14 occur occur VERB ct-9406 27 15 , , PUNCT ct-9406 27 16 especially especially ADV ct-9406 27 17 in in ADP ct-9406 27 18 a a DET ct-9406 27 19 cell cell NOUN ct-9406 27 20 as as ADV ct-9406 27 21 highly highly ADV ct-9406 27 22 specialized specialized ADJ ct-9406 27 23 as as ADP ct-9406 27 24 sperm sperm NOUN ct-9406 27 25 . . PUNCT ct-9406 28 1 capacitation capacitation NOUN ct-9406 28 2 is be AUX ct-9406 28 3 the the DET ct-9406 28 4 maturational maturational ADJ ct-9406 28 5 process process NOUN ct-9406 28 6 that that PRON ct-9406 28 7 sensitizes sensitize VERB ct-9406 28 8 sperm sperm NOUN ct-9406 28 9 to to PART ct-9406 28 10 recognize recognize VERB ct-9406 28 11 and and CCONJ ct-9406 28 12 fertilize fertilize VERB ct-9406 28 13 the the DET ct-9406 28 14 oocyte oocyte NOUN ct-9406 28 15 ; ; PUNCT ct-9406 28 16 this this DET ct-9406 28 17 process process NOUN ct-9406 28 18 causes cause VERB ct-9406 28 19 substantial substantial ADJ ct-9406 28 20 plasma plasma NOUN ct-9406 28 21 membrane membrane NOUN ct-9406 28 22 modifications modification NOUN ct-9406 28 23 . . PUNCT ct-9406 29 1 capacitation capacitation NOUN ct-9406 29 2 involves involve VERB ct-9406 29 3 the the DET ct-9406 29 4 removal removal NOUN ct-9406 29 5 of of ADP ct-9406 29 6 cholesterol cholesterol NOUN ct-9406 29 7 from from ADP ct-9406 29 8 the the DET ct-9406 29 9 plasma plasma NOUN ct-9406 29 10 membrane membrane NOUN ct-9406 29 11 , , PUNCT ct-9406 29 12 removal removal NOUN ct-9406 29 13 of of ADP ct-9406 29 14 coating coat VERB ct-9406 29 15 materials material NOUN ct-9406 29 16 from from ADP ct-9406 29 17 the the DET ct-9406 29 18 membrane membrane NOUN ct-9406 29 19 , , PUNCT ct-9406 29 20 a a DET ct-9406 29 21 rise rise NOUN ct-9406 29 22 in in ADP ct-9406 29 23 intracellular intracellular ADJ ct-9406 29 24 ca2 ca2 PROPN ct-9406 29 25 + + PROPN ct-9406 29 26 , , PUNCT ct-9406 29 27 an an DET ct-9406 29 28 increase increase NOUN ct-9406 29 29 in in ADP ct-9406 29 30 intracellular intracellular ADJ ct-9406 29 31 camp camp NOUN ct-9406 29 32 , , PUNCT ct-9406 29 33 and and CCONJ ct-9406 29 34 a a DET ct-9406 29 35 dramatic dramatic ADJ ct-9406 29 36 increase increase NOUN ct-9406 29 37 in in ADP ct-9406 29 38 tyrosine tyrosine NOUN ct-9406 29 39 phosphorylation phosphorylation NOUN ct-9406 29 40 . . PUNCT ct-9406 30 1 removal removal NOUN ct-9406 30 2 of of ADP ct-9406 30 3 cholesterol cholesterol NOUN ct-9406 30 4 from from ADP ct-9406 30 5 the the DET ct-9406 30 6 membrane membrane NOUN ct-9406 30 7 is be AUX ct-9406 30 8 preceded precede VERB ct-9406 30 9 by by ADP ct-9406 30 10 its its PRON ct-9406 30 11 oxidation oxidation NOUN ct-9406 30 12 , , PUNCT ct-9406 30 13 stimulated stimulate VERB ct-9406 30 14 by by ADP ct-9406 30 15 bicarbonate bicarbonate NOUN ct-9406 30 16 , , PUNCT ct-9406 30 17 and and CCONJ ct-9406 30 18 the the DET ct-9406 30 19 formation formation NOUN ct-9406 30 20 of of ADP ct-9406 30 21 oxysterols,29–31 oxysterols,29–31 PROPN ct-9406 30 22 which which PRON ct-9406 30 23 are be AUX ct-9406 30 24 depleted deplete VERB ct-9406 30 25 from from ADP ct-9406 30 26 the the DET ct-9406 30 27 sperm sperm NOUN ct-9406 30 28 membrane membrane NOUN ct-9406 30 29 by by ADP ct-9406 30 30 albumin albumin NOUN ct-9406 30 31 . . PUNCT ct-9406 31 1 the the DET ct-9406 31 2 process process NOUN ct-9406 31 3 prepares prepare VERB ct-9406 31 4 the the DET ct-9406 31 5 sperm sperm NOUN ct-9406 31 6 to to PART ct-9406 31 7 fertilize fertilize VERB ct-9406 31 8 an an DET ct-9406 31 9 oocyte oocyte NOUN ct-9406 31 10 ; ; PUNCT ct-9406 31 11 however however ADV ct-9406 31 12 , , PUNCT ct-9406 31 13 most most ADJ ct-9406 31 14 of of ADP ct-9406 31 15 the the DET ct-9406 31 16 sperm sperm NOUN ct-9406 31 17 in in ADP ct-9406 31 18 the the DET ct-9406 31 19 ejaculate ejaculate NOUN ct-9406 31 20 will will AUX ct-9406 31 21 never never ADV ct-9406 31 22 reach reach VERB ct-9406 31 23 an an DET ct-9406 31 24 egg egg NOUN ct-9406 31 25 and and CCONJ ct-9406 31 26 will will AUX ct-9406 31 27 die die VERB ct-9406 31 28 within within ADP ct-9406 31 29 the the DET ct-9406 31 30 female female ADJ ct-9406 31 31 reproductive reproductive ADJ ct-9406 31 32 tract tract NOUN ct-9406 31 33 and and CCONJ ct-9406 31 34 only only ADV ct-9406 31 35 a a DET ct-9406 31 36 small small ADJ ct-9406 31 37 subpopulation subpopulation NOUN ct-9406 31 38 of of ADP ct-9406 31 39 sperm sperm NOUN ct-9406 31 40 fully fully ADV ct-9406 31 41 capacitate,32,33 capacitate,32,33 X ct-9406 31 42 thus thus ADV ct-9406 31 43 mechanisms mechanism NOUN ct-9406 31 44 for for ADP ct-9406 31 45 immunologically immunologically ADV ct-9406 31 46 silent silent ADJ ct-9406 31 47 elimination elimination NOUN ct-9406 31 48 of of ADP ct-9406 31 49 redundant redundant ADJ ct-9406 31 50 sperm sperm NOUN ct-9406 31 51 are be AUX ct-9406 31 52 likely likely ADJ ct-9406 31 53 . . PUNCT ct-9406 32 1 capacitation capacitation NOUN ct-9406 32 2 shares share VERB ct-9406 32 3 molecular molecular ADJ ct-9406 32 4 mechanisms mechanism NOUN ct-9406 32 5 with with ADP ct-9406 32 6 what what PRON ct-9406 32 7 begins begin VERB ct-9406 32 8 to to PART ct-9406 32 9 be be AUX ct-9406 32 10 considered consider VERB ct-9406 32 11 special special ADJ ct-9406 32 12 forms form NOUN ct-9406 32 13 of of ADP ct-9406 32 14 programmed program VERB ct-9406 32 15 cell cell NOUN ct-9406 32 16 death death NOUN ct-9406 32 17 in in ADP ct-9406 32 18 sperm sperm NOUN ct-9406 32 19 : : PUNCT ct-9406 32 20 redox redox NOUN ct-9406 32 21 - - PUNCT ct-9406 32 22 regulated regulate VERB ct-9406 32 23 , , PUNCT ct-9406 32 24 intracellular intracellular ADJ ct-9406 32 25 ca2 ca2 PROPN ct-9406 32 26 + + NOUN ct-9406 32 27 increase increase NOUN ct-9406 32 28 and and CCONJ ct-9406 32 29 membrane membrane NOUN ct-9406 32 30 destabilization destabilization NOUN ct-9406 32 31 occur occur VERB ct-9406 32 32 . . PUNCT ct-9406 33 1 however however ADV ct-9406 33 2 , , PUNCT ct-9406 33 3 capacitation capacitation NOUN ct-9406 33 4 implies imply VERB ct-9406 33 5 a a DET ct-9406 33 6 dramatic dramatic ADJ ct-9406 33 7 increase increase NOUN ct-9406 33 8 in in ADP ct-9406 33 9 tyrosine tyrosine NOUN ct-9406 33 10 phosphorylation phosphorylation NOUN ct-9406 33 11 and and CCONJ ct-9406 33 12 changes change NOUN ct-9406 33 13 in in ADP ct-9406 33 14 the the DET ct-9406 33 15 pattern pattern NOUN ct-9406 33 16 of of ADP ct-9406 33 17 motility motility NOUN ct-9406 33 18 to to PART ct-9406 33 19 hyperactivated hyperactivate VERB ct-9406 33 20 motility motility NOUN ct-9406 33 21 , , PUNCT ct-9406 33 22 whereas whereas SCONJ ct-9406 33 23 programmed program VERB ct-9406 33 24 cell cell NOUN ct-9406 33 25 death death NOUN ct-9406 33 26 ends end VERB ct-9406 33 27 with with ADP ct-9406 33 28 cessation cessation NOUN ct-9406 33 29 of of ADP ct-9406 33 30 any any DET ct-9406 33 31 kind kind NOUN ct-9406 33 32 of of ADP ct-9406 33 33 motility motility NOUN ct-9406 33 34 in in ADP ct-9406 33 35 sperm sperm NOUN ct-9406 33 36 . . PUNCT ct-9406 34 1 finally finally ADV ct-9406 34 2 , , PUNCT ct-9406 34 3 apoptotic apoptotic ADJ ct-9406 34 4 sperm sperm NOUN ct-9406 34 5 release release NOUN ct-9406 34 6 ‘ ' PUNCT ct-9406 34 7 find find VERB ct-9406 34 8 me i PRON ct-9406 34 9 / / SYM ct-9406 34 10 eat eat VERB ct-9406 34 11 me i PRON ct-9406 34 12 ’ ' PUNCT ct-9406 34 13 signals signal NOUN ct-9406 34 14 to to PART ct-9406 34 15 achieve achieve VERB ct-9406 34 16 a a DET ct-9406 34 17 silent silent ADJ ct-9406 34 18 removal removal NOUN ct-9406 34 19 of of ADP ct-9406 34 20 redundant redundant ADJ ct-9406 34 21 sperm sperm NOUN ct-9406 34 22 . . PUNCT ct-9406 35 1 the the DET ct-9406 35 2 inhibition inhibition NOUN ct-9406 35 3 of of ADP ct-9406 35 4 glutathione glutathione NOUN ct-9406 35 5     SPACE ct-9406 35 6 peroxidase peroxidase NOUN ct-9406 35 7     SPACE ct-9406 35 8 4 4 NUM ct-9406 35 9 ( ( PUNCT ct-9406 35 10 gpx4 gpx4 PROPN ct-9406 35 11 ) ) PUNCT ct-9406 35 12 leads lead VERB ct-9406 35 13 to to ADP ct-9406 35 14 a a DET ct-9406 35 15 specific specific ADJ ct-9406 35 16 form form NOUN ct-9406 35 17 of of ADP ct-9406 35 18 cellular cellular ADJ ct-9406 35 19 demise demise NOUN ct-9406 35 20 termed term VERB ct-9406 35 21 ferroptosis.34 ferroptosis.34 ADV ct-9406 35 22 this this PRON ct-9406 35 23 is be AUX ct-9406 35 24 a a DET ct-9406 35 25 form form NOUN ct-9406 35 26 of of ADP ct-9406 35 27 programmed program VERB ct-9406 35 28 cell cell NOUN ct-9406 35 29 death death NOUN ct-9406 35 30 that that PRON ct-9406 35 31 is be AUX ct-9406 35 32 characterized characterize VERB ct-9406 35 33 by by ADP ct-9406 35 34 the the DET ct-9406 35 35 failure failure NOUN ct-9406 35 36 of of ADP ct-9406 35 37 glutathione glutathione NOUN ct-9406 35 38 ( ( PUNCT ct-9406 35 39 gsh)-dependent gsh)-dependent ADJ ct-9406 35 40 antioxidant antioxidant NOUN ct-9406 35 41 defenses defense NOUN ct-9406 35 42 that that PRON ct-9406 35 43 can can AUX ct-9406 35 44 be be AUX ct-9406 35 45 triggered trigger VERB ct-9406 35 46 by by ADP ct-9406 35 47 the the DET ct-9406 35 48 inhibition inhibition NOUN ct-9406 35 49 of of ADP ct-9406 35 50 gpx4 gpx4 NOUN ct-9406 35 51 or or CCONJ ct-9406 35 52 slcta11 slcta11 VERB ct-9406 35 53 / / SYM ct-9406 36 1 xct xct PROPN ct-9406 36 2 , , PUNCT ct-9406 36 3 the the DET ct-9406 36 4 antiporter antiporter NOUN ct-9406 36 5 that that PRON ct-9406 36 6 exchanges exchange NOUN ct-9406 36 7 extracellular extracellular ADJ ct-9406 36 8 cystine cystine NOUN ct-9406 36 9 for for ADP ct-9406 36 10 intracellular intracellular ADJ ct-9406 36 11 glutamate glutamate NOUN ct-9406 36 12 . . PUNCT ct-9406 37 1 cystine cystine NOUN ct-9406 37 2 is be AUX ct-9406 37 3 used use VERB ct-9406 37 4 for for ADP ct-9406 37 5 gsh gsh PROPN ct-9406 37 6 synthesis synthesis NOUN ct-9406 37 7 , , PUNCT ct-9406 37 8 a a DET ct-9406 37 9 mechanism mechanism NOUN ct-9406 37 10 recently recently ADV ct-9406 37 11 described describe VERB ct-9406 37 12 in in ADP ct-9406 37 13 stallion stallion NOUN ct-9406 37 14 sperm.27,35 sperm.27,35 NOUN ct-9406 37 15 it it PRON ct-9406 37 16 is be AUX ct-9406 37 17 possible possible ADJ ct-9406 37 18 that that SCONJ ct-9406 37 19 this this DET ct-9406 37 20 form form NOUN ct-9406 37 21 of of ADP ct-9406 37 22 cell cell NOUN ct-9406 37 23 death death NOUN ct-9406 37 24 is be AUX ct-9406 37 25 present present ADJ ct-9406 37 26 in in ADP ct-9406 37 27 sperm.26 sperm.26 ADJ ct-9406 37 28 induction induction NOUN ct-9406 37 29 of of ADP ct-9406 37 30 apoptosis apoptosis NOUN ct-9406 37 31 or or CCONJ ct-9406 37 32 necrosis necrosis NOUN ct-9406 37 33 in in ADP ct-9406 37 34 sperm sperm NOUN ct-9406 37 35 if if SCONJ ct-9406 37 36 the the DET ct-9406 37 37 presence presence NOUN ct-9406 37 38 of of ADP ct-9406 37 39 apoptosis apoptosis NOUN ct-9406 37 40 in in ADP ct-9406 37 41 ejaculated ejaculated ADJ ct-9406 37 42 sperm sperm NOUN ct-9406 37 43 is be AUX ct-9406 37 44 a a DET ct-9406 37 45 remnant remnant NOUN ct-9406 37 46 of of ADP ct-9406 37 47 spermatogenesis spermatogenesis NOUN ct-9406 37 48 ( ( PUNCT ct-9406 37 49 as as SCONJ ct-9406 37 50 stated state VERB ct-9406 37 51 by by ADP ct-9406 37 52 the the DET ct-9406 37 53 abortive abortive ADJ ct-9406 37 54 apoptosis apoptosis NOUN ct-9406 37 55 theory theory NOUN ct-9406 37 56 ) ) PUNCT ct-9406 37 57 , , PUNCT ct-9406 37 58 most most ADJ ct-9406 37 59 sperm sperm NOUN ct-9406 37 60 are be AUX ct-9406 37 61 already already ADV ct-9406 37 62 programmed program VERB ct-9406 37 63 to to PART ct-9406 37 64 experience experience VERB ct-9406 37 65 programmed program VERB ct-9406 37 66 cell cell NOUN ct-9406 37 67 death death NOUN ct-9406 37 68 ; ; PUNCT ct-9406 37 69 thus thus ADV ct-9406 37 70 , , PUNCT ct-9406 37 71 the the DET ct-9406 37 72 concept concept NOUN ct-9406 37 73 is be AUX ct-9406 37 74 that that SCONJ ct-9406 37 75 more more ADV ct-9406 37 76 than than ADP ct-9406 37 77 induced induce VERB ct-9406 37 78 , , PUNCT ct-9406 37 79 in in ADP ct-9406 37 80 sperm sperm NOUN ct-9406 37 81 , , PUNCT ct-9406 37 82 the the DET ct-9406 37 83 programmed program VERB ct-9406 37 84 cell cell NOUN ct-9406 37 85 death death NOUN ct-9406 37 86 process process NOUN ct-9406 37 87 can can AUX ct-9406 37 88 be be AUX ct-9406 37 89 accelerated accelerate VERB ct-9406 37 90 . . PUNCT ct-9406 38 1 however however ADV ct-9406 38 2 , , PUNCT ct-9406 38 3 classical classical ADJ ct-9406 38 4 inductors inductor NOUN ct-9406 38 5 of of ADP ct-9406 38 6 apoptosis apoptosis NOUN ct-9406 38 7 have have AUX ct-9406 38 8 been be AUX ct-9406 38 9 successfully successfully ADV ct-9406 38 10 used use VERB ct-9406 38 11 in in ADP ct-9406 38 12 stallion stallion NOUN ct-9406 38 13 sperm sperm NOUN ct-9406 38 14 , , PUNCT ct-9406 38 15 although although SCONJ ct-9406 38 16 betulinic betulinic ADJ ct-9406 38 17 acid acid PROPN ct-9406 38 18 seems seem VERB ct-9406 38 19 more more ADV ct-9406 38 20 effective effective ADJ ct-9406 38 21 to to PART ct-9406 38 22 induce induce VERB ct-9406 38 23 apoptosis apoptosis NOUN ct-9406 38 24 than than ADP ct-9406 38 25 staurosporine staurosporine NOUN ct-9406 38 26 or or CCONJ ct-9406 38 27 thapsigargin,36 thapsigargin,36 NUM ct-9406 38 28 which which PRON ct-9406 38 29 is be AUX ct-9406 38 30 in in ADP ct-9406 38 31 agreement agreement NOUN ct-9406 38 32 with with ADP ct-9406 38 33 the the DET ct-9406 38 34 predominance predominance NOUN ct-9406 38 35 on on ADP ct-9406 38 36 the the DET ct-9406 38 37 mitochondrial mitochondrial ADJ ct-9406 38 38 pathway pathway NOUN ct-9406 38 39 of of ADP ct-9406 38 40 apoptosis apoptosis NOUN ct-9406 38 41 in in ADP ct-9406 38 42 sperm.37 sperm.37 PROPN ct-9406 38 43 in in ADP ct-9406 38 44 sperm sperm NOUN ct-9406 38 45 , , PUNCT ct-9406 38 46 cryopreservation cryopreservation NOUN ct-9406 38 47 has have AUX ct-9406 38 48 been be AUX ct-9406 38 49 constantly constantly ADV ct-9406 38 50 associated associate VERB ct-9406 38 51 with with ADP ct-9406 38 52 the the DET ct-9406 38 53 production production NOUN ct-9406 38 54 of of ADP ct-9406 38 55 apoptotic apoptotic ADJ ct-9406 38 56 changes change NOUN ct-9406 38 57 related relate VERB ct-9406 38 58 to to ADP ct-9406 38 59 the the DET ct-9406 38 60 oxidative oxidative ADJ ct-9406 38 61 stress stress NOUN ct-9406 38 62 caused cause VERB ct-9406 38 63 by by ADP ct-9406 38 64 freezing freeze VERB ct-9406 38 65 and and CCONJ ct-9406 38 66 thawing thawing NOUN ct-9406 38 67 . . PUNCT ct-9406 39 1 changes change NOUN ct-9406 39 2 in in ADP ct-9406 39 3 the the DET ct-9406 39 4 integrity integrity NOUN ct-9406 39 5 of of ADP ct-9406 39 6 the the DET ct-9406 39 7 plasma plasma NOUN ct-9406 39 8 membrane membrane NOUN ct-9406 39 9 although although SCONJ ct-9406 39 10 the the DET ct-9406 39 11 integrity integrity NOUN ct-9406 39 12 of of ADP ct-9406 39 13 the the DET ct-9406 39 14 plasma plasma NOUN ct-9406 39 15 membrane membrane NOUN ct-9406 39 16 is be AUX ct-9406 39 17 rapidly rapidly ADV ct-9406 39 18 lost lose VERB ct-9406 39 19 when when SCONJ ct-9406 39 20 necrotic necrotic ADJ ct-9406 39 21 cell cell NOUN ct-9406 39 22 death death NOUN ct-9406 39 23 occurs occur VERB ct-9406 39 24 , , PUNCT ct-9406 39 25 subtle subtle ADJ ct-9406 39 26 changes change NOUN ct-9406 39 27 occur occur VERB ct-9406 39 28 under under ADP ct-9406 39 29 other other ADJ ct-9406 39 30 forms form NOUN ct-9406 39 31 of of ADP ct-9406 39 32 sperm sperm NOUN ct-9406 39 33 demise demise NOUN ct-9406 39 34 . . PUNCT ct-9406 40 1 the the DET ct-9406 40 2 traditional traditional ADJ ct-9406 40 3 approach approach NOUN ct-9406 40 4 for for ADP ct-9406 40 5 the the DET ct-9406 40 6 determination determination NOUN ct-9406 40 7 of of ADP ct-9406 40 8 viability viability NOUN ct-9406 40 9 in in ADP ct-9406 40 10 sperm sperm NOUN ct-9406 40 11 biology biology NOUN ct-9406 40 12 has have AUX ct-9406 40 13 been be AUX ct-9406 40 14 using use VERB ct-9406 40 15 dye dye NOUN ct-9406 40 16 exclusion exclusion NOUN ct-9406 40 17 assays assay NOUN ct-9406 40 18 ; ; PUNCT ct-9406 40 19 the the DET ct-9406 40 20 combination combination NOUN ct-9406 40 21 of of ADP ct-9406 40 22 the the DET ct-9406 40 23 permeable permeable ADJ ct-9406 40 24 dna dna NOUN ct-9406 40 25 binding bind VERB ct-9406 40 26 probe probe NOUN ct-9406 40 27 sybr-14 sybr-14 PROPN ct-9406 40 28 and and CCONJ ct-9406 40 29 the the DET ct-9406 40 30 impermeable impermeable ADJ ct-9406 40 31 dna dna NOUN ct-9406 40 32 binding bind VERB ct-9406 40 33 probe probe NOUN ct-9406 40 34 propidium propidium NOUN ct-9406 40 35 iodide iodide NOUN ct-9406 40 36 is be AUX ct-9406 40 37 widely widely ADV ct-9406 40 38 used use VERB ct-9406 40 39 and and CCONJ ct-9406 40 40 is be AUX ct-9406 40 41 marketed market VERB ct-9406 40 42 as as ADP ct-9406 40 43 a a DET ct-9406 40 44 kit kit NOUN ct-9406 40 45 for for ADP ct-9406 40 46 sperm sperm NOUN ct-9406 40 47 viability viability NOUN ct-9406 40 48 assay assay VERB ct-9406 40 49 . . PUNCT ct-9406 41 1 this this DET ct-9406 41 2 assay assay NOUN ct-9406 41 3 became become VERB ct-9406 41 4 very very ADV ct-9406 41 5 popular popular ADJ ct-9406 41 6 , , PUNCT ct-9406 41 7 especially especially ADV ct-9406 41 8 in in ADP ct-9406 41 9 the the DET ct-9406 41 10 veterinary veterinary ADJ ct-9406 41 11 world world NOUN ct-9406 41 12 , , PUNCT ct-9406 41 13 since since SCONJ ct-9406 41 14 its its PRON ct-9406 41 15 introduction introduction NOUN ct-9406 41 16 in in ADP ct-9406 41 17 the the DET ct-9406 41 18 early early ADJ ct-9406 41 19 90s 90 NOUN ct-9406 41 20 of of ADP ct-9406 41 21 the the DET ct-9406 41 22 past past ADJ ct-9406 41 23 century century NOUN ct-9406 41 24 . . PUNCT ct-9406 42 1 this this DET ct-9406 42 2 combination combination NOUN ct-9406 42 3 was be AUX ct-9406 42 4 appropriate appropriate ADJ ct-9406 42 5 for for SCONJ ct-9406 42 6 the the DET ct-9406 42 7 single single ADJ ct-9406 42 8 laser laser NOUN ct-9406 42 9 ( ( PUNCT ct-9406 42 10 blue blue ADJ ct-9406 42 11 ) ) PUNCT ct-9406 42 12 of of ADP ct-9406 42 13 the the DET ct-9406 42 14 cytometers cytometer NOUN ct-9406 42 15 commonly commonly ADV ct-9406 42 16 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 VERB ct-9406 42 17 citation citation NOUN ct-9406 42 18 line line NOUN ct-9406 42 19 : : PUNCT ct-9406 42 20 clinical clinical ADJ ct-9406 42 21 theriogenology theriogenology NOUN ct-9406 42 22 2023 2023 NUM ct-9406 42 23 , , PUNCT ct-9406 42 24 15 15 NUM ct-9406 42 25 , , PUNCT ct-9406 42 26 9406 9406 NUM ct-9406 42 27 , , PUNCT ct-9406 42 28 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 NOUN ct-9406 42 29 3 3 NUM ct-9406 42 30 used use VERB ct-9406 42 31 in in ADP ct-9406 42 32 the the DET ct-9406 42 33 andrology andrology NOUN ct-9406 42 34 labs lab NOUN ct-9406 42 35 at at ADP ct-9406 42 36 that that DET ct-9406 42 37 time time NOUN ct-9406 42 38 . . PUNCT ct-9406 43 1 during during ADP ct-9406 43 2 the the DET ct-9406 43 3 first first ADJ ct-9406 43 4 decade decade NOUN ct-9406 43 5 of of ADP ct-9406 43 6 the the DET ct-9406 43 7 present present ADJ ct-9406 43 8 century century NOUN ct-9406 43 9 , , PUNCT ct-9406 43 10 more more ADV ct-9406 43 11 sophisticated sophisticated ADJ ct-9406 43 12 assays assay NOUN ct-9406 43 13 started start VERB ct-9406 43 14 to to PART ct-9406 43 15 be be AUX ct-9406 43 16 introduced introduce VERB ct-9406 43 17 in in ADP ct-9406 43 18 spermatology spermatology NOUN ct-9406 43 19 ; ; PUNCT ct-9406 43 20 these these DET ct-9406 43 21 techniques technique NOUN ct-9406 43 22 allowed allow VERB ct-9406 43 23 to to PART ct-9406 43 24 disclose disclose VERB ct-9406 43 25 distinct distinct ADJ ct-9406 43 26 physiological physiological ADJ ct-9406 43 27 states state NOUN ct-9406 43 28 of of ADP ct-9406 43 29 the the DET ct-9406 43 30 membranes membrane NOUN ct-9406 43 31 in in ADP ct-9406 43 32 addition addition NOUN ct-9406 43 33 to to ADP ct-9406 43 34 necrotic necrotic ADJ ct-9406 43 35 cells cell NOUN ct-9406 43 36 , , PUNCT ct-9406 43 37 and and CCONJ ct-9406 43 38 these these DET ct-9406 43 39 assays assay NOUN ct-9406 43 40 identified identify VERB ct-9406 43 41 the the DET ct-9406 43 42 permeability permeability NOUN ct-9406 43 43 of of ADP ct-9406 43 44 the the DET ct-9406 43 45 membranes membrane NOUN ct-9406 43 46 , , PUNCT ct-9406 43 47 the the DET ct-9406 43 48 order order NOUN ct-9406 43 49 of of ADP ct-9406 43 50 the the DET ct-9406 43 51 lipid lipid NOUN ct-9406 43 52 packaging packaging NOUN ct-9406 43 53 , , PUNCT ct-9406 43 54 the the DET ct-9406 43 55 transposition transposition NOUN ct-9406 43 56 of of ADP ct-9406 43 57 specific specific ADJ ct-9406 43 58 phospholipids phospholipid NOUN ct-9406 43 59 , , PUNCT ct-9406 43 60 the the DET ct-9406 43 61 polarity polarity NOUN ct-9406 43 62 of of ADP ct-9406 43 63 the the DET ct-9406 43 64 membrane membrane NOUN ct-9406 43 65 , , PUNCT ct-9406 43 66 and and CCONJ ct-9406 43 67 assays assay NOUN ct-9406 43 68 able able ADJ ct-9406 43 69 to to PART ct-9406 43 70 identify identify VERB ct-9406 43 71 the the DET ct-9406 43 72 peroxidation peroxidation NOUN ct-9406 43 73 of of ADP ct-9406 43 74 membrane membrane NOUN ct-9406 43 75 lipids lipid NOUN ct-9406 43 76 . . PUNCT ct-9406 44 1 assays assay NOUN ct-9406 44 2 identifying identify VERB ct-9406 44 3 changes change NOUN ct-9406 44 4 in in ADP ct-9406 44 5 membrane membrane NOUN ct-9406 44 6 permeability permeability NOUN ct-9406 44 7 the the DET ct-9406 44 8 probe probe NOUN ct-9406 44 9 that that PRON ct-9406 44 10 has have AUX ct-9406 44 11 gained gain VERB ct-9406 44 12 popularity popularity NOUN ct-9406 44 13 in in ADP ct-9406 44 14 the the DET ct-9406 44 15 spermatology spermatology NOUN ct-9406 44 16 field field NOUN ct-9406 44 17 is be AUX ct-9406 44 18 yopro-1 yopro-1 PROPN ct-9406 44 19 . . PUNCT ct-9406 45 1 initially initially ADV ct-9406 45 2 , , PUNCT ct-9406 45 3 this this DET ct-9406 45 4 probe probe NOUN ct-9406 45 5 was be AUX ct-9406 45 6 used use VERB ct-9406 45 7 as as ADP ct-9406 45 8 an an DET ct-9406 45 9 alternative alternative NOUN ct-9406 45 10 to to ADP ct-9406 45 11 red red ADJ ct-9406 45 12 emission emission NOUN ct-9406 45 13 viability viability NOUN ct-9406 45 14 dyes dye NOUN ct-9406 45 15 , , PUNCT ct-9406 45 16 when when SCONJ ct-9406 45 17 merocyanine-540 merocyanine-540 ADJ ct-9406 45 18 was be AUX ct-9406 45 19 used.38–40 used.38–40 PRON ct-9406 45 20 however however ADV ct-9406 45 21 , , PUNCT ct-9406 45 22 yopro-1 yopro-1 NUM ct-9406 45 23 not not PART ct-9406 45 24 only only ADV ct-9406 45 25 is be AUX ct-9406 45 26 able able ADJ ct-9406 45 27 to to PART ct-9406 45 28 stain stain VERB ct-9406 45 29 necrotic necrotic ADJ ct-9406 45 30 cells cell NOUN ct-9406 45 31 but but CCONJ ct-9406 45 32 can can AUX ct-9406 45 33 also also ADV ct-9406 45 34 use use VERB ct-9406 45 35 specific specific ADJ ct-9406 45 36 channels channel NOUN ct-9406 45 37 to to PART ct-9406 45 38 cross cross VERB ct-9406 45 39 intact intact ADJ ct-9406 45 40 membranes.41–43 membranes.41–43 NOUN ct-9406 45 41 moreover moreover ADV ct-9406 45 42 , , PUNCT ct-9406 45 43 live live ADJ ct-9406 45 44 sperm sperm NOUN ct-9406 45 45 may may AUX ct-9406 45 46 pump pump VERB ct-9406 45 47 out out ADP ct-9406 45 48 yopro-1 yopro-1 NUM ct-9406 45 49 , , PUNCT ct-9406 45 50 whereas whereas SCONJ ct-9406 45 51 depletion depletion NOUN ct-9406 45 52 of of ADP ct-9406 45 53 atp atp PROPN ct-9406 45 54 may may AUX ct-9406 45 55 result result VERB ct-9406 45 56 in in ADP ct-9406 45 57 accumulation accumulation NOUN ct-9406 45 58 of of ADP ct-9406 45 59 yopro-1 yopro-1 NUM ct-9406 45 60 in in ADP ct-9406 45 61 the the DET ct-9406 45 62 sperm.44 sperm.44 NOUN ct-9406 45 63 this this DET ct-9406 45 64 property property NOUN ct-9406 45 65 allows allow VERB ct-9406 45 66 the the DET ct-9406 45 67 identification identification NOUN ct-9406 45 68 of of ADP ct-9406 45 69 sperm sperm NOUN ct-9406 45 70 , , PUNCT ct-9406 45 71 with with ADP ct-9406 45 72 intact intact ADJ ct-9406 45 73 membranes membrane NOUN ct-9406 45 74 , , PUNCT ct-9406 45 75 but but CCONJ ct-9406 45 76 in in ADP ct-9406 45 77 a a DET ct-9406 45 78 compromised compromise VERB ct-9406 45 79 physiological physiological ADJ ct-9406 45 80 situation situation NOUN ct-9406 45 81 ; ; PUNCT ct-9406 45 82 therefore therefore ADV ct-9406 45 83 , , PUNCT ct-9406 45 84 this this DET ct-9406 45 85 dye dye NOUN ct-9406 45 86 is be AUX ct-9406 45 87 used use VERB ct-9406 45 88 to to PART ct-9406 45 89 identify identify VERB ct-9406 45 90 apoptotic apoptotic ADJ ct-9406 45 91 cells cell NOUN ct-9406 45 92 and and CCONJ ct-9406 45 93 to to PART ct-9406 45 94 identify identify VERB ct-9406 45 95 increased increase VERB ct-9406 45 96 membrane membrane NOUN ct-9406 45 97 permeability permeability NOUN ct-9406 45 98 due due ADP ct-9406 45 99 to to ADP ct-9406 45 100 lipid lipid NOUN ct-9406 45 101 peroxidation peroxidation NOUN ct-9406 45 102 . . PUNCT ct-9406 46 1 an an DET ct-9406 46 2 interesting interesting ADJ ct-9406 46 3 report report NOUN ct-9406 46 4 uses use VERB ct-9406 46 5 the the DET ct-9406 46 6 yopro-1 yopro-1 NUM ct-9406 46 7 assay assay NOUN ct-9406 46 8 to to PART ct-9406 46 9 sort sort VERB ct-9406 46 10 sperm sperm NOUN ct-9406 46 11 of of ADP ct-9406 46 12 better well ADJ ct-9406 46 13 quality quality NOUN ct-9406 46 14 ( ( PUNCT ct-9406 46 15 low low ADJ ct-9406 46 16 dna dna PROPN ct-9406 46 17 fragmentation fragmentation NOUN ct-9406 46 18 ) ) PUNCT ct-9406 46 19 in in ADP ct-9406 46 20 human human ADJ ct-9406 46 21 sperm sperm NOUN ct-9406 46 22 since since SCONJ ct-9406 46 23 dna dna PROPN ct-9406 46 24 fragmentation fragmentation NOUN ct-9406 46 25 of of ADP ct-9406 46 26 the the DET ct-9406 46 27 dna dna PROPN ct-9406 46 28 in in ADP ct-9406 46 29 sperm sperm NOUN ct-9406 46 30 is be AUX ct-9406 46 31 due due ADJ ct-9406 46 32 to to ADP ct-9406 46 33 oxidative oxidative ADJ ct-9406 46 34 attack attack NOUN ct-9406 46 35 , , PUNCT ct-9406 46 36 sperm sperm NOUN ct-9406 46 37 experiencing experience VERB ct-9406 46 38 oxidative oxidative ADJ ct-9406 46 39 stress stress NOUN ct-9406 46 40 also also ADV ct-9406 46 41 have have AUX ct-9406 46 42 increased increase VERB ct-9406 46 43 membrane membrane NOUN ct-9406 46 44 permeability,45 permeability,45 NOUN ct-9406 46 45 and and CCONJ ct-9406 46 46 these these DET ct-9406 46 47 sperm sperm NOUN ct-9406 46 48 can can AUX ct-9406 46 49 be be AUX ct-9406 46 50 easily easily ADV ct-9406 46 51 identified identify VERB ct-9406 46 52 and and CCONJ ct-9406 46 53 removed.46 removed.46 NOUN ct-9406 46 54 lipid lipid NOUN ct-9406 46 55 peroxidation peroxidation NOUN ct-9406 46 56 of of ADP ct-9406 46 57 the the DET ct-9406 46 58 membrane membrane NOUN ct-9406 46 59 increases increase VERB ct-9406 46 60 membrane membrane ADJ ct-9406 46 61 permeability permeability NOUN ct-9406 46 62 : : PUNCT ct-9406 46 63 assays assay NOUN ct-9406 46 64 identifying identify VERB ct-9406 46 65 the the DET ct-9406 46 66 transposition transposition NOUN ct-9406 46 67 of of ADP ct-9406 46 68 phospholipids phospholipid NOUN ct-9406 46 69 among among ADP ct-9406 46 70 the the DET ct-9406 46 71 changes change NOUN ct-9406 46 72 that that PRON ct-9406 46 73 oxidative oxidative ADJ ct-9406 46 74 stress stress NOUN ct-9406 46 75 induces induce NOUN ct-9406 46 76 is be AUX ct-9406 46 77 the the DET ct-9406 46 78 increased increase VERB ct-9406 46 79 permeability permeability NOUN ct-9406 46 80 of of ADP ct-9406 46 81 the the DET ct-9406 46 82 plasmalemma.45 plasmalemma.45 NOUN ct-9406 46 83 lipid lipid NOUN ct-9406 46 84 peroxidation peroxidation NOUN ct-9406 46 85 is be AUX ct-9406 46 86 thus thus ADV ct-9406 46 87 the the DET ct-9406 46 88 consequence consequence NOUN ct-9406 46 89 of of ADP ct-9406 46 90 oxidative oxidative ADJ ct-9406 46 91 stress stress NOUN ct-9406 46 92 and and CCONJ ct-9406 46 93 is be AUX ct-9406 46 94 a a DET ct-9406 46 95 chain chain NOUN ct-9406 46 96 reaction reaction NOUN ct-9406 46 97 , , PUNCT ct-9406 46 98 with with ADP ct-9406 46 99 peroxidation peroxidation NOUN ct-9406 46 100 products product NOUN ct-9406 46 101 created create VERB ct-9406 46 102 close close ADV ct-9406 46 103 to to ADP ct-9406 46 104 each each DET ct-9406 46 105 other other ADJ ct-9406 46 106 . . PUNCT ct-9406 47 1 the the DET ct-9406 47 2 identification identification NOUN ct-9406 47 3 of of ADP ct-9406 47 4 the the DET ct-9406 47 5 transposition transposition NOUN ct-9406 47 6 of of ADP ct-9406 47 7 specific specific ADJ ct-9406 47 8 phospholipids phospholipid NOUN ct-9406 47 9 from from ADP ct-9406 47 10 the the DET ct-9406 47 11 inner inner NOUN ct-9406 47 12 to to ADP ct-9406 47 13 the the DET ct-9406 47 14 outer outer ADJ ct-9406 47 15 membrane membrane NOUN ct-9406 47 16 constitutes constitute VERB ct-9406 47 17 a a DET ct-9406 47 18 classical classical ADJ ct-9406 47 19 assay assay NOUN ct-9406 47 20 for for ADP ct-9406 47 21 the the DET ct-9406 47 22 detection detection NOUN ct-9406 47 23 of of ADP ct-9406 47 24 apoptotic apoptotic ADJ ct-9406 47 25 cells cell NOUN ct-9406 47 26 . . PUNCT ct-9406 48 1 the the DET ct-9406 48 2 annexin annexin NOUN ct-9406 48 3 - - PUNCT ct-9406 48 4 v v NOUN ct-9406 48 5 assay assay NOUN ct-9406 48 6 is be AUX ct-9406 48 7 a a DET ct-9406 48 8 popular popular ADJ ct-9406 48 9 technique technique NOUN ct-9406 48 10 to to PART ct-9406 48 11 identify identify VERB ct-9406 48 12 the the DET ct-9406 48 13 presence presence NOUN ct-9406 48 14 of of ADP ct-9406 48 15 phosphatidylserine phosphatidylserine NOUN ct-9406 48 16 ( ( PUNCT ct-9406 48 17 ps ps NOUN ct-9406 48 18 ) ) PUNCT ct-9406 48 19 in in ADP ct-9406 48 20 the the DET ct-9406 48 21 outer outer ADJ ct-9406 48 22 leaflet leaflet NOUN ct-9406 48 23 of of ADP ct-9406 48 24 the the DET ct-9406 48 25 plasmalemma plasmalemma PROPN ct-9406 48 26 . . PUNCT ct-9406 49 1 under under ADP ct-9406 49 2 normal normal ADJ ct-9406 49 3 conditions condition NOUN ct-9406 49 4 , , PUNCT ct-9406 49 5 ps ps PROPN ct-9406 49 6 and and CCONJ ct-9406 49 7 phosphatidylethanolamine phosphatidylethanolamine PROPN ct-9406 49 8 are be AUX ct-9406 49 9 present present ADJ ct-9406 49 10 in in ADP ct-9406 49 11 the the DET ct-9406 49 12 interleaflet interleaflet NOUN ct-9406 49 13 of of ADP ct-9406 49 14 the the DET ct-9406 49 15 plasmalemma plasmalemma PROPN ct-9406 49 16 . . PUNCT ct-9406 50 1 when when SCONJ ct-9406 50 2 the the DET ct-9406 50 3 cell cell NOUN ct-9406 50 4 enters enter VERB ct-9406 50 5 apoptosis apoptosis NOUN ct-9406 50 6 , , PUNCT ct-9406 50 7 ps ps PROPN ct-9406 50 8 translocated translocate VERB ct-9406 50 9 to to ADP ct-9406 50 10 the the DET ct-9406 50 11 outer outer ADJ ct-9406 50 12 membrane membrane NOUN ct-9406 50 13 constituting constitute VERB ct-9406 50 14 an an DET ct-9406 50 15 ‘ ' PUNCT ct-9406 50 16 eat eat VERB ct-9406 50 17 me i PRON ct-9406 50 18 ’ ' PUNCT ct-9406 50 19 signaling signal VERB ct-9406 50 20 ( ( PUNCT ct-9406 50 21 presence presence NOUN ct-9406 50 22 of of ADP ct-9406 50 23 ps ps PROPN ct-9406 50 24 signals signal VERB ct-9406 50 25 the the DET ct-9406 50 26 cells cell NOUN ct-9406 50 27 that that PRON ct-9406 50 28 will will AUX ct-9406 50 29 be be AUX ct-9406 50 30 phagocytosed phagocytose VERB ct-9406 50 31 in in ADP ct-9406 50 32 a a DET ct-9406 50 33 silent silent ADJ ct-9406 50 34 , , PUNCT ct-9406 50 35 noninflammatory noninflammatory ADJ ct-9406 50 36 manner manner NOUN ct-9406 50 37 ) ) PUNCT ct-9406 50 38 . . PUNCT ct-9406 51 1 this this PRON ct-9406 51 2 is be AUX ct-9406 51 3 especially especially ADV ct-9406 51 4 important important ADJ ct-9406 51 5 for for ADP ct-9406 51 6 the the DET ct-9406 51 7 elimination elimination NOUN ct-9406 51 8 of of ADP ct-9406 51 9 redundant redundant ADJ ct-9406 51 10 sperm sperm NOUN ct-9406 51 11 from from ADP ct-9406 51 12 the the DET ct-9406 51 13 female female ADJ ct-9406 51 14 genitalia genitalia NOUN ct-9406 51 15 , , PUNCT ct-9406 51 16 and and CCONJ ct-9406 51 17 failure failure NOUN ct-9406 51 18 in in ADP ct-9406 51 19 this this DET ct-9406 51 20 mechanism mechanism NOUN ct-9406 51 21 may may AUX ct-9406 51 22 lead lead VERB ct-9406 51 23 to to ADP ct-9406 51 24 an an DET ct-9406 51 25 uncontrolled uncontrolled ADJ ct-9406 51 26 inflammatory inflammatory ADJ ct-9406 51 27 reaction reaction NOUN ct-9406 51 28 to to ADP ct-9406 51 29 semen semen NOUN ct-9406 51 30 and and CCONJ ct-9406 51 31 posterior posterior ADJ ct-9406 51 32 endometritis endometritis NOUN ct-9406 51 33 . . PUNCT ct-9406 52 1 polarity polarity NOUN ct-9406 52 2 of of ADP ct-9406 52 3 the the DET ct-9406 52 4 plasma plasma NOUN ct-9406 52 5 membrane membrane NOUN ct-9406 52 6 an an DET ct-9406 52 7 anionic anionic ADJ ct-9406 52 8 bis bis ADJ ct-9406 52 9 oxonol oxonol NOUN ct-9406 52 10 dye dye NOUN ct-9406 52 11 ( ( PUNCT ct-9406 52 12 disbac2)47 disbac2)47 PROPN ct-9406 52 13 enters enter VERB ct-9406 52 14 depolarized depolarized ADJ ct-9406 52 15 cells cell NOUN ct-9406 52 16 where where SCONJ ct-9406 52 17 they they PRON ct-9406 52 18 bind bind VERB ct-9406 52 19 to to ADP ct-9406 52 20 the the DET ct-9406 52 21 intracellular intracellular ADJ ct-9406 52 22 proteins protein NOUN ct-9406 52 23 of of ADP ct-9406 52 24 membranes membrane NOUN ct-9406 52 25 and and CCONJ ct-9406 52 26 exhibit exhibit VERB ct-9406 52 27 enhanced enhance VERB ct-9406 52 28 fluorescence fluorescence NOUN ct-9406 52 29 and and CCONJ ct-9406 52 30 red red ADJ ct-9406 52 31 - - PUNCT ct-9406 52 32 orange orange NOUN ct-9406 52 33 spectral spectral ADJ ct-9406 52 34 shifts shift NOUN ct-9406 52 35 . . PUNCT ct-9406 53 1 increased increase VERB ct-9406 53 2 depolarization depolarization NOUN ct-9406 53 3 results result NOUN ct-9406 53 4 in in ADP ct-9406 53 5 more more ADJ ct-9406 53 6 influx influx NOUN ct-9406 53 7 of of ADP ct-9406 53 8 the the DET ct-9406 53 9 anionic anionic ADJ ct-9406 53 10 dye dye NOUN ct-9406 53 11 , , PUNCT ct-9406 53 12 and and CCONJ ct-9406 53 13 thus thus ADV ct-9406 53 14 an an DET ct-9406 53 15 increase increase NOUN ct-9406 53 16 in in ADP ct-9406 53 17 fluorescence fluorescence NOUN ct-9406 53 18 . . PUNCT ct-9406 54 1 conversely conversely ADV ct-9406 54 2 , , PUNCT ct-9406 54 3 hyperpolarization hyperpolarization NOUN ct-9406 54 4 is be AUX ct-9406 54 5 indicated indicate VERB ct-9406 54 6 by by ADP ct-9406 54 7 a a DET ct-9406 54 8 decrease decrease NOUN ct-9406 54 9 in in ADP ct-9406 54 10 fluorescence fluorescence NOUN ct-9406 54 11 . . PUNCT ct-9406 55 1 in in ADP ct-9406 55 2 contrast contrast NOUN ct-9406 55 3 to to ADP ct-9406 55 4 cationic cationic ADJ ct-9406 55 5 carbocyanines carbocyanine NOUN ct-9406 55 6 , , PUNCT ct-9406 55 7 anionic anionic ADJ ct-9406 55 8 bis bis X ct-9406 55 9 oxonols oxonol NOUN ct-9406 55 10 are be AUX ct-9406 55 11 largely largely ADV ct-9406 55 12 excluded exclude VERB ct-9406 55 13 from from ADP ct-9406 55 14 mitochondria mitochondria NOUN ct-9406 55 15 and and CCONJ ct-9406 55 16 are be AUX ct-9406 55 17 primarily primarily ADV ct-9406 55 18 sensitive sensitive ADJ ct-9406 55 19 to to ADP ct-9406 55 20 plasma plasma NOUN ct-9406 55 21 membrane membrane NOUN ct-9406 55 22 potential.48 potential.48 NOUN ct-9406 55 23 the the DET ct-9406 55 24 use use NOUN ct-9406 55 25 of of ADP ct-9406 55 26 flow flow NOUN ct-9406 55 27 cytometry cytometry NOUN ct-9406 55 28 allows allow VERB ct-9406 55 29 determining determine VERB ct-9406 55 30 , , PUNCT ct-9406 55 31 at at ADP ct-9406 55 32 the the DET ct-9406 55 33 single single ADJ ct-9406 55 34 cell cell NOUN ct-9406 55 35 level level NOUN ct-9406 55 36 , , PUNCT ct-9406 55 37 many many ADJ ct-9406 55 38 distinct distinct ADJ ct-9406 55 39 characteristics characteristic NOUN ct-9406 55 40 of of ADP ct-9406 55 41 sperm sperm NOUN ct-9406 55 42 . . PUNCT ct-9406 56 1 recently recently ADV ct-9406 56 2 , , PUNCT ct-9406 56 3 changes change NOUN ct-9406 56 4 in in ADP ct-9406 56 5 the the DET ct-9406 56 6 potential potential NOUN ct-9406 56 7 of of ADP ct-9406 56 8 the the DET ct-9406 56 9 membrane membrane NOUN ct-9406 56 10 have have AUX ct-9406 56 11 been be AUX ct-9406 56 12 studied study VERB ct-9406 56 13 in in ADP ct-9406 56 14 relation relation NOUN ct-9406 56 15 to to ADP ct-9406 56 16 capacitation capacitation NOUN ct-9406 56 17 and and CCONJ ct-9406 56 18 cryopreservation.33,49 cryopreservation.33,49 NOUN ct-9406 56 19 these these DET ct-9406 56 20 changes change NOUN ct-9406 56 21 relate relate VERB ct-9406 56 22 to to ADP ct-9406 56 23 modifications modification NOUN ct-9406 56 24 in in ADP ct-9406 56 25 intracellular intracellular ADJ ct-9406 56 26 na+ na+ NOUN ct-9406 56 27 that that PRON ct-9406 56 28 can can AUX ct-9406 56 29 also also ADV ct-9406 56 30 be be AUX ct-9406 56 31 monitored monitor VERB ct-9406 56 32 using use VERB ct-9406 56 33 flow flow NOUN ct-9406 56 34 cytometry cytometry NOUN ct-9406 56 35 . . PUNCT ct-9406 57 1 the the DET ct-9406 57 2 potential potential NOUN ct-9406 57 3 of of ADP ct-9406 57 4 the the DET ct-9406 57 5 sperm sperm NOUN ct-9406 57 6 membrane membrane NOUN ct-9406 57 7 can can AUX ct-9406 57 8 be be AUX ct-9406 57 9 determined determine VERB ct-9406 57 10 with with ADP ct-9406 57 11 the the DET ct-9406 57 12 probe probe NOUN ct-9406 57 13 disbac2 disbac2 PROPN ct-9406 57 14 combined combine VERB ct-9406 57 15 with with ADP ct-9406 57 16 a a DET ct-9406 57 17 vitality vitality NOUN ct-9406 57 18 dye dye NOUN ct-9406 57 19 to to PART ct-9406 57 20 exclude exclude VERB ct-9406 57 21 dead dead ADJ ct-9406 57 22 sperm sperm NOUN ct-9406 57 23 from from ADP ct-9406 57 24 the the DET ct-9406 57 25 analysis analysis NOUN ct-9406 57 26 . . PUNCT ct-9406 58 1 depolarization depolarization NOUN ct-9406 58 2 of of ADP ct-9406 58 3 the the DET ct-9406 58 4 membrane membrane NOUN ct-9406 58 5 has have AUX ct-9406 58 6 been be AUX ct-9406 58 7 reported report VERB ct-9406 58 8 in in ADP ct-9406 58 9 relation relation NOUN ct-9406 58 10 to to ADP ct-9406 58 11 apoptotic apoptotic ADJ ct-9406 58 12 changes,49 changes,49 NOUN ct-9406 58 13 whereas whereas SCONJ ct-9406 58 14 capacitation capacitation NOUN ct-9406 58 15 is be AUX ct-9406 58 16 linked link VERB ct-9406 58 17 to to ADP ct-9406 58 18 hyperpolarization.33 hyperpolarization.33 NOUN ct-9406 58 19 interestingly interestingly ADV ct-9406 58 20 , , PUNCT ct-9406 58 21 this this PRON ct-9406 58 22 occurs occur VERB ct-9406 58 23 only only ADV ct-9406 58 24 in in ADP ct-9406 58 25 a a DET ct-9406 58 26 subpopulation subpopulation NOUN ct-9406 58 27 of of ADP ct-9406 58 28 live live ADJ ct-9406 58 29 , , PUNCT ct-9406 58 30 intact intact ADJ ct-9406 58 31 acrosome acrosome NOUN ct-9406 58 32 sperm sperm NOUN ct-9406 58 33 and and CCONJ ct-9406 58 34 not not PART ct-9406 58 35 in in ADP ct-9406 58 36 the the DET ct-9406 58 37 whole whole ADJ ct-9406 58 38 ejaculate ejaculate NOUN ct-9406 58 39 . . PUNCT ct-9406 59 1 depolarization depolarization NOUN ct-9406 59 2 of of ADP ct-9406 59 3 the the DET ct-9406 59 4 membrane membrane NOUN ct-9406 59 5 has have AUX ct-9406 59 6 been be AUX ct-9406 59 7 related relate VERB ct-9406 59 8 to to ADP ct-9406 59 9 apoptotic apoptotic ADJ ct-9406 59 10 changes change NOUN ct-9406 59 11 in in ADP ct-9406 59 12 somatic somatic ADJ ct-9406 59 13 cells cell NOUN ct-9406 59 14 and and CCONJ ct-9406 59 15 in in ADP ct-9406 59 16 sperm sperm NOUN ct-9406 59 17 . . PUNCT ct-9406 60 1 thus thus ADV ct-9406 60 2 , , PUNCT ct-9406 60 3 combined combined ADJ ct-9406 60 4 staining staining NOUN ct-9406 60 5 has have AUX ct-9406 60 6 been be AUX ct-9406 60 7 employed employ VERB ct-9406 60 8 to to PART ct-9406 60 9 determine determine VERB ct-9406 60 10 if if SCONJ ct-9406 60 11 depolarized depolarized ADJ ct-9406 60 12 sperm sperm NOUN ct-9406 60 13 correspond correspond VERB ct-9406 60 14 also also ADV ct-9406 60 15 to to ADP ct-9406 60 16 sperm sperm VERB ct-9406 60 17 having have VERB ct-9406 60 18 apoptotic apoptotic ADJ ct-9406 60 19 features feature NOUN ct-9406 60 20 . . PUNCT ct-9406 61 1 these these DET ct-9406 61 2 experiments experiment NOUN ct-9406 61 3 demonstrate demonstrate VERB ct-9406 61 4 that that SCONJ ct-9406 61 5 depolarized depolarized ADJ ct-9406 61 6 sperm sperm NOUN ct-9406 61 7 are be AUX ct-9406 61 8 also also ADV ct-9406 61 9 caspase caspase NOUN ct-9406 61 10 3 3 NUM ct-9406 61 11 positive positive ADJ ct-9406 61 12 , , PUNCT ct-9406 61 13 express express ADJ ct-9406 61 14 ps ps NOUN ct-9406 61 15 translocation translocation NOUN ct-9406 61 16 , , PUNCT ct-9406 61 17 and and CCONJ ct-9406 61 18 have have AUX ct-9406 61 19 increased increase VERB ct-9406 61 20 intracellular intracellular ADJ ct-9406 61 21 na+ na+ NOUN ct-9406 61 22 and and CCONJ ct-9406 61 23 collapsed collapse VERB ct-9406 61 24 mitochondria.49 mitochondria.49 VERB ct-9406 61 25 active active ADJ ct-9406 61 26 sperm sperm NOUN ct-9406 61 27 caspases caspase NOUN ct-9406 61 28 : : PUNCT ct-9406 61 29 denouncing denounce VERB ct-9406 61 30 sperm sperm NOUN ct-9406 61 31 senescence senescence NOUN ct-9406 61 32 senescent senescent ADJ ct-9406 61 33 sperm sperm NOUN ct-9406 61 34 express express VERB ct-9406 61 35 active active ADJ ct-9406 61 36 caspase caspase NOUN ct-9406 61 37 3.37,50,51 3.37,50,51 NUM ct-9406 61 38 depending depend VERB ct-9406 61 39 of of ADP ct-9406 61 40 the the DET ct-9406 61 41 presence presence NOUN ct-9406 61 42 of of ADP ct-9406 61 43 prosurvival prosurvival NOUN ct-9406 61 44 factors factor NOUN ct-9406 61 45 , , PUNCT ct-9406 61 46 caspase caspase NOUN ct-9406 61 47 3 3 NUM ct-9406 61 48 remains remain VERB ct-9406 61 49 inactive inactive ADJ ct-9406 61 50 due due ADP ct-9406 61 51 to to ADP ct-9406 61 52 the the DET ct-9406 61 53 phosphorylation phosphorylation NOUN ct-9406 61 54 of of ADP ct-9406 61 55 protein protein NOUN ct-9406 61 56 kinase kinase PROPN ct-9406 61 57 b b PROPN ct-9406 61 58 ( ( PUNCT ct-9406 61 59 pkb pkb PROPN ct-9406 61 60 or or CCONJ ct-9406 61 61 akt).43,51,52 akt).43,51,52 NOUN ct-9406 61 62 if if SCONJ ct-9406 61 63 prosurvival prosurvival NOUN ct-9406 61 64 factors factor NOUN ct-9406 61 65 are be AUX ct-9406 61 66 lost lose VERB ct-9406 61 67 or or CCONJ ct-9406 61 68 oxidative oxidative ADJ ct-9406 61 69 stress stress NOUN ct-9406 61 70 reaches reach VERB ct-9406 61 71 a a DET ct-9406 61 72 threshold threshold NOUN ct-9406 61 73 , , PUNCT ct-9406 61 74 caspase caspase NOUN ct-9406 61 75 3 3 NUM ct-9406 61 76 is be AUX ct-9406 61 77 activated activate VERB ct-9406 61 78 and and CCONJ ct-9406 61 79 sperm sperm NOUN ct-9406 61 80 senescence senescence NOUN ct-9406 61 81 and and CCONJ ct-9406 61 82 death death NOUN ct-9406 61 83 are be AUX ct-9406 61 84 triggered.37,53 triggered.37,53 NOUN ct-9406 61 85 cryopreservation cryopreservation NOUN ct-9406 61 86 triggers trigger VERB ct-9406 61 87 this this DET ct-9406 61 88 phenomenon phenomenon NOUN ct-9406 61 89 , , PUNCT ct-9406 61 90 and and CCONJ ct-9406 61 91 surviving survive VERB ct-9406 61 92 sperm sperm NOUN ct-9406 61 93 experience experience NOUN ct-9406 61 94 accelerated accelerate VERB ct-9406 61 95 senescence.52,54–57 senescence.52,54–57 ADJ ct-9406 61 96 active active ADJ ct-9406 61 97 caspase caspase NOUN ct-9406 61 98 3 3 NUM ct-9406 61 99 can can AUX ct-9406 61 100 be be AUX ct-9406 61 101 detected detect VERB ct-9406 61 102 using use VERB ct-9406 61 103 cellevent cellevent NOUN ct-9406 61 104 caspase-3 caspase-3 NUM ct-9406 61 105 green green ADJ ct-9406 61 106 detection detection NOUN ct-9406 61 107 reagent reagent NOUN ct-9406 61 108 , , PUNCT ct-9406 61 109 which which PRON ct-9406 61 110 consists consist VERB ct-9406 61 111 of of ADP ct-9406 61 112 a a DET ct-9406 61 113 four four NUM ct-9406 61 114 - - PUNCT ct-9406 61 115 amino amino NOUN ct-9406 61 116 - - PUNCT ct-9406 61 117 acid acid NOUN ct-9406 61 118 peptide peptide NOUN ct-9406 61 119 ( ( PUNCT ct-9406 61 120 devd devd NOUN ct-9406 61 121 ) ) PUNCT ct-9406 61 122 conjugated conjugate VERB ct-9406 61 123 to to ADP ct-9406 61 124 a a DET ct-9406 61 125 nucleic nucleic ADJ ct-9406 61 126 acid acid NOUN ct-9406 61 127 - - PUNCT ct-9406 61 128 binding bind VERB ct-9406 61 129 dye dye NOUN ct-9406 61 130 . . PUNCT ct-9406 62 1 this this DET ct-9406 62 2 cell cell NOUN ct-9406 62 3 - - PUNCT ct-9406 62 4 permeant permeant ADJ ct-9406 62 5 substrate substrate NOUN ct-9406 62 6 is be AUX ct-9406 62 7 intrinsically intrinsically ADV ct-9406 62 8 nonfluorescent nonfluorescent ADJ ct-9406 62 9 because because SCONJ ct-9406 62 10 the the DET ct-9406 62 11 devd devd NOUN ct-9406 62 12 peptide peptide NOUN ct-9406 62 13 inhibits inhibit VERB ct-9406 62 14 the the DET ct-9406 62 15 ability ability NOUN ct-9406 62 16 of of ADP ct-9406 62 17 the the DET ct-9406 62 18 dye dye NOUN ct-9406 62 19 to to PART ct-9406 62 20 bind bind VERB ct-9406 62 21 to to ADP ct-9406 62 22 dna dna PROPN ct-9406 62 23 . . PUNCT ct-9406 63 1 after after ADP ct-9406 63 2 the the DET ct-9406 63 3 activation activation NOUN ct-9406 63 4 of of ADP ct-9406 63 5 caspase caspase PROPN ct-9406 63 6 3 3 NUM ct-9406 63 7 in in ADP ct-9406 63 8 apoptotic apoptotic ADJ ct-9406 63 9 cells cell NOUN ct-9406 63 10 , , PUNCT ct-9406 63 11 the the DET ct-9406 63 12 devd devd NOUN ct-9406 63 13 peptide peptide NOUN ct-9406 63 14 is be AUX ct-9406 63 15 cleaved cleave VERB ct-9406 63 16 , , PUNCT ct-9406 63 17 enabling enable VERB ct-9406 63 18 the the DET ct-9406 63 19 dye dye NOUN ct-9406 63 20 to to PART ct-9406 63 21 bind bind VERB ct-9406 63 22 to to ADP ct-9406 63 23 dna dna NOUN ct-9406 63 24 and and CCONJ ct-9406 63 25 produce produce VERB ct-9406 63 26 a a DET ct-9406 63 27 bright bright ADJ ct-9406 63 28 , , PUNCT ct-9406 63 29 fluorogenic fluorogenic ADJ ct-9406 63 30 response response NOUN ct-9406 63 31 with with ADP ct-9406 63 32 an an DET ct-9406 63 33 absorption absorption NOUN ct-9406 63 34 / / SYM ct-9406 63 35 emission emission NOUN ct-9406 63 36 maxima maxima NOUN ct-9406 63 37 of of ADP ct-9406 63 38 502/530 502/530 NUM ct-9406 63 39 nm nm NOUN ct-9406 63 40 . . PUNCT ct-9406 64 1 flow flow NOUN ct-9406 64 2 cytometry cytometry PROPN ct-9406 64 3 data datum NOUN ct-9406 64 4 output output NOUN ct-9406 64 5 and and CCONJ ct-9406 64 6 clinical clinical ADJ ct-9406 64 7 interpretation interpretation NOUN ct-9406 64 8 an an DET ct-9406 64 9 example example NOUN ct-9406 64 10 ( ( PUNCT ct-9406 64 11 figure figure NOUN ct-9406 64 12 1 1 NUM ct-9406 64 13 ) ) PUNCT ct-9406 64 14 of of ADP ct-9406 64 15 a a DET ct-9406 64 16 multiparametric multiparametric ADJ ct-9406 64 17 panel panel NOUN ct-9406 64 18 as as ADP ct-9406 64 19 an an DET ct-9406 64 20 output output NOUN ct-9406 64 21 of of ADP ct-9406 64 22 stallion stallion NOUN ct-9406 64 23 sperm sperm NOUN ct-9406 64 24 studied study VERB ct-9406 64 25 with with ADP ct-9406 64 26 a a DET ct-9406 64 27 modern modern ADJ ct-9406 64 28 flow flow NOUN ct-9406 64 29 cytometer cytometer NOUN ct-9406 64 30 ( ( PUNCT ct-9406 64 31 cytoflex cytoflex PROPN ct-9406 64 32 lx lx NOUN ct-9406 64 33 ) ) PUNCT ct-9406 64 34 , , PUNCT ct-9406 64 35 providing provide VERB ct-9406 64 36 data datum NOUN ct-9406 64 37 relevant relevant ADJ ct-9406 64 38 for for SCONJ ct-9406 64 39 the the DET ct-9406 64 40 clinician clinician NOUN ct-9406 64 41 to to PART ct-9406 64 42 evaluate evaluate VERB ct-9406 64 43 the the DET ct-9406 64 44 current current ADJ ct-9406 64 45 and and CCONJ ct-9406 64 46 potential potential ADJ ct-9406 64 47 status status NOUN ct-9406 64 48 of of ADP ct-9406 64 49 a a DET ct-9406 64 50 semen semen NOUN ct-9406 64 51 sample sample NOUN ct-9406 64 52 . . PUNCT ct-9406 65 1 description description NOUN ct-9406 65 2 of of ADP ct-9406 65 3 this this DET ct-9406 65 4 four four NUM ct-9406 65 5 - - PUNCT ct-9406 65 6 color color NOUN ct-9406 65 7 experiment experiment NOUN ct-9406 65 8 implies imply VERB ct-9406 65 9 that that SCONJ ct-9406 65 10 the the DET ct-9406 65 11 information information NOUN ct-9406 65 12 provided provide VERB ct-9406 65 13 has have VERB ct-9406 65 14 to to PART ct-9406 65 15 be be AUX ct-9406 65 16 depicted depict VERB ct-9406 65 17 in in ADP ct-9406 65 18 several several ADJ ct-9406 65 19 dot dot NOUN ct-9406 65 20 plots plot NOUN ct-9406 65 21 since since SCONJ ct-9406 65 22 the the DET ct-9406 65 23 number number NOUN ct-9406 65 24 of of ADP ct-9406 65 25 2d 2d NOUN ct-9406 65 26 plots plot NOUN ct-9406 65 27 increases increase NOUN ct-9406 65 28 exponentially exponentially ADV ct-9406 65 29 with with ADP ct-9406 65 30 the the DET ct-9406 65 31 number number NOUN ct-9406 65 32 of of ADP ct-9406 65 33 colors color NOUN ct-9406 65 34 in in ADP ct-9406 65 35 the the DET ct-9406 65 36 analysis analysis NOUN ct-9406 65 37 . . PUNCT ct-9406 66 1 additionally additionally ADV ct-9406 66 2 , , PUNCT ct-9406 66 3 the the DET ct-9406 66 4 establishment establishment NOUN ct-9406 66 5 of of ADP ct-9406 66 6 gates gate NOUN ct-9406 66 7 is be AUX ct-9406 66 8 performed perform VERB ct-9406 66 9 manually manually ADV ct-9406 66 10 . . PUNCT ct-9406 67 1 these these PRON ct-9406 67 2 are be AUX ct-9406 67 3 confounding confound VERB ct-9406 67 4 data datum NOUN ct-9406 67 5 for for ADP ct-9406 67 6 a a DET ct-9406 67 7 nonspecialist nonspecialist NOUN ct-9406 67 8 and and CCONJ ct-9406 67 9 ought ought AUX ct-9406 67 10 to to PART ct-9406 67 11 be be AUX ct-9406 67 12 simplified simplify VERB ct-9406 67 13 without without ADP ct-9406 67 14 losing lose VERB ct-9406 67 15 relevant relevant ADJ ct-9406 67 16 information information NOUN ct-9406 67 17 , , PUNCT ct-9406 67 18 for for ADP ct-9406 67 19 instance instance NOUN ct-9406 67 20 , , PUNCT ct-9406 67 21 using use VERB ct-9406 67 22 self self NOUN ct-9406 67 23 - - PUNCT ct-9406 67 24 learning learn VERB ct-9406 67 25 artificial artificial ADJ ct-9406 67 26 intelligence intelligence NOUN ct-9406 67 27 techniques technique NOUN ct-9406 67 28 . . PUNCT ct-9406 68 1 an an DET ct-9406 68 2 example example NOUN ct-9406 68 3 of of ADP ct-9406 68 4 such such ADJ ct-9406 68 5 is be AUX ct-9406 68 6 presented present VERB ct-9406 68 7 ( ( PUNCT ct-9406 68 8 figure figure NOUN ct-9406 68 9 2 2 NUM ct-9406 68 10 ) ) PUNCT ct-9406 68 11 , , PUNCT ct-9406 68 12 using use VERB ct-9406 68 13 the the DET ct-9406 68 14 flo flo ADJ ct-9406 68 15 self self NOUN ct-9406 68 16 - - PUNCT ct-9406 68 17 organizing organize VERB ct-9406 68 18 maps map NOUN ct-9406 68 19 for for ADP ct-9406 68 20 visualization visualization NOUN ct-9406 68 21 and and CCONJ ct-9406 68 22 organization organization NOUN ct-9406 68 23 of of ADP ct-9406 68 24 flow flow NOUN ct-9406 68 25 cytometry cytometry NOUN ct-9406 68 26 data datum NOUN ct-9406 68 27 . . PUNCT ct-9406 69 1 with with ADP ct-9406 69 2 this this DET ct-9406 69 3 technique technique NOUN ct-9406 69 4 , , PUNCT ct-9406 69 5 all all DET ct-9406 69 6 the the DET ct-9406 69 7 characteristics characteristic NOUN ct-9406 69 8 of of ADP ct-9406 69 9 the the DET ct-9406 69 10 sperm sperm NOUN ct-9406 69 11 in in ADP ct-9406 69 12 the the DET ct-9406 69 13 sample sample NOUN ct-9406 69 14 can can AUX ct-9406 69 15 be be AUX ct-9406 69 16 visualized visualize VERB ct-9406 69 17 at at ADP ct-9406 69 18 a a DET ct-9406 69 19 glance glance NOUN ct-9406 69 20 , , PUNCT ct-9406 69 21 facilitating facilitate VERB ct-9406 69 22 comparisons comparison NOUN ct-9406 69 23 among among ADP ct-9406 69 24 samples sample NOUN ct-9406 69 25 and and CCONJ ct-9406 69 26 identification identification NOUN ct-9406 69 27 of of ADP ct-9406 69 28 subtle subtle ADJ ct-9406 69 29 changes change NOUN ct-9406 69 30 . . PUNCT ct-9406 70 1 in in ADP ct-9406 70 2 the the DET ct-9406 70 3 example example NOUN ct-9406 70 4 , , PUNCT ct-9406 70 5 aliquots aliquot NOUN ct-9406 70 6 of of ADP ct-9406 70 7 the the DET ct-9406 70 8 same same ADJ ct-9406 70 9 stallion stallion NOUN ct-9406 70 10 ejaculate ejaculate NOUN ct-9406 70 11 were be AUX ct-9406 70 12 extended extend VERB ct-9406 70 13 and and CCONJ ct-9406 70 14 incubated incubate VERB ct-9406 70 15 at at ADP ct-9406 70 16 37ºc 37ºc NOUN ct-9406 70 17 for for ADP ct-9406 70 18 3 3 NUM ct-9406 70 19 hours hour NOUN ct-9406 70 20 in in ADP ct-9406 70 21 2 2 NUM ct-9406 70 22 distinct distinct ADJ ct-9406 70 23 media medium NOUN ct-9406 70 24 . . PUNCT ct-9406 71 1 sperm sperm NOUN ct-9406 71 2 were be AUX ct-9406 71 3 stained stain VERB ct-9406 71 4 with with ADP ct-9406 71 5 a a DET ct-9406 71 6 4 4 NUM ct-9406 71 7 colors color NOUN ct-9406 71 8 protocol protocol NOUN ct-9406 71 9 , , PUNCT ct-9406 71 10 to to PART ct-9406 71 11 identify identify VERB ct-9406 71 12 live live ADV ct-9406 71 13 , , PUNCT ct-9406 71 14 apoptotic apoptotic ADJ ct-9406 71 15 ( ( PUNCT ct-9406 71 16 caspase caspase NOUN ct-9406 71 17 3 3 NUM ct-9406 71 18 and and CCONJ ct-9406 71 19 annexin annexin NOUN ct-9406 71 20 v v NOUN ct-9406 71 21 ) ) PUNCT ct-9406 71 22 and and CCONJ ct-9406 71 23 necrotic necrotic ADJ ct-9406 71 24 sperm sperm NOUN ct-9406 71 25 ( ( PUNCT ct-9406 71 26 live live ADJ ct-9406 71 27 / / SYM ct-9406 71 28 dead dead ADJ ct-9406 71 29 viakrom viakrom NOUN ct-9406 71 30 808 808 NUM ct-9406 71 31 ® ® NOUN ct-9406 71 32 ) ) PUNCT ct-9406 71 33 . . PUNCT ct-9406 72 1 a a DET ct-9406 72 2 quick quick ADJ ct-9406 72 3 view view NOUN ct-9406 72 4 of of ADP ct-9406 72 5 this this DET ct-9406 72 6 panel panel NOUN ct-9406 72 7 , , PUNCT ct-9406 72 8 comparing compare VERB ct-9406 72 9 the the DET ct-9406 72 10 2 2 NUM ct-9406 72 11 incubation incubation NOUN ct-9406 72 12 sets set NOUN ct-9406 72 13 ( ( PUNCT ct-9406 72 14 1 1 NUM ct-9406 72 15 versus versus ADP ct-9406 72 16 2 2 NUM ct-9406 72 17 ) ) PUNCT ct-9406 72 18 , , PUNCT ct-9406 72 19 allows allow VERB ct-9406 72 20 the the DET ct-9406 72 21 observer observer NOUN ct-9406 72 22 to to PART ct-9406 72 23 see see VERB ct-9406 72 24 that that SCONJ ct-9406 72 25 there there PRON ct-9406 72 26 are be VERB ct-9406 72 27 more more ADV ct-9406 72 28 viable viable ADJ ct-9406 72 29 sperma sperma NOUN ct-9406 72 30 in in ADP ct-9406 72 31 1a 1a NOUN ct-9406 72 32 than than ADP ct-9406 72 33 in in ADP ct-9406 72 34 mapping mapping NOUN ct-9406 72 35 2 2 NUM ct-9406 72 36 . . PUNCT ct-9406 73 1 in in ADP ct-9406 73 2 the the DET ct-9406 73 3 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 NOUN ct-9406 73 4 4 4 NUM ct-9406 73 5 citation citation NOUN ct-9406 73 6 line line NOUN ct-9406 73 7 : : PUNCT ct-9406 73 8 clinical clinical ADJ ct-9406 73 9 theriogenology theriogenology NOUN ct-9406 73 10 2023 2023 NUM ct-9406 73 11 , , PUNCT ct-9406 73 12 15 15 NUM ct-9406 73 13 , , PUNCT ct-9406 73 14 9406 9406 NUM ct-9406 73 15 , , PUNCT ct-9406 73 16 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 AUX ct-9406 73 17 figure figure VERB ct-9406 73 18 1 1 NUM ct-9406 73 19 . . NOUN ct-9406 73 20 example example NOUN ct-9406 73 21 of of ADP ct-9406 73 22 a a DET ct-9406 73 23 multiparametric multiparametric ADJ ct-9406 73 24 panel panel NOUN ct-9406 73 25 for for ADP ct-9406 73 26 the the DET ct-9406 73 27 study study NOUN ct-9406 73 28 of of ADP ct-9406 73 29 stallion stallion NOUN ct-9406 73 30 sperm sperm NOUN ct-9406 73 31 . . PUNCT ct-9406 74 1 ( ( PUNCT ct-9406 74 2 a a X ct-9406 74 3 ) ) PUNCT ct-9406 74 4 gate gate NOUN ct-9406 74 5 for for ADP ct-9406 74 6 the the DET ct-9406 74 7 sperm sperm NOUN ct-9406 74 8 , , PUNCT ct-9406 74 9 where where SCONJ ct-9406 74 10 sperm sperm NOUN ct-9406 74 11 events event NOUN ct-9406 74 12 are be AUX ct-9406 74 13 identified identify VERB ct-9406 74 14 based base VERB ct-9406 74 15 on on ADP ct-9406 74 16 forward forward ADV ct-9406 74 17 ( ( PUNCT ct-9406 74 18 fsc fsc PROPN ct-9406 74 19 ) ) PUNCT ct-9406 74 20 and and CCONJ ct-9406 74 21 side side NOUN ct-9406 74 22 scatter scatter NOUN ct-9406 74 23 ; ; PUNCT ct-9406 74 24 events event NOUN ct-9406 74 25 having have VERB ct-9406 74 26 higher high ADJ ct-9406 74 27 area area NOUN ct-9406 74 28 in in ADP ct-9406 74 29 the the DET ct-9406 74 30 fsc fsc PROPN ct-9406 74 31 - - PUNCT ct-9406 74 32 a a NOUN ct-9406 74 33 are be AUX ct-9406 74 34 removed remove VERB ct-9406 74 35 from from ADP ct-9406 74 36 the the DET ct-9406 74 37 analysis analysis NOUN ct-9406 74 38 since since SCONJ ct-9406 74 39 they they PRON ct-9406 74 40 represent represent VERB ct-9406 74 41 doublets doublet NOUN ct-9406 74 42 and and CCONJ ct-9406 74 43 clumps clump NOUN ct-9406 74 44 . . PUNCT ct-9406 75 1 ( ( PUNCT ct-9406 75 2 b b X ct-9406 75 3 ) ) PUNCT ct-9406 75 4 to to PART ct-9406 75 5 remove remove VERB ct-9406 75 6 debris debris NOUN ct-9406 75 7 , , PUNCT ct-9406 75 8 the the DET ct-9406 75 9 sample sample NOUN ct-9406 75 10 is be AUX ct-9406 75 11 stained stain VERB ct-9406 75 12 with with ADP ct-9406 75 13 a a DET ct-9406 75 14 permeable permeable ADJ ct-9406 75 15 dna dna NOUN ct-9406 75 16 binding bind VERB ct-9406 75 17 probe probe NOUN ct-9406 75 18 ( ( PUNCT ct-9406 75 19 hoechst hoechst NOUN ct-9406 75 20 33342 33342 NUM ct-9406 75 21 ) ) PUNCT ct-9406 75 22 , , PUNCT ct-9406 75 23 and and CCONJ ct-9406 75 24 only only ADV ct-9406 75 25 dna dna NOUN ct-9406 75 26 bearing bear VERB ct-9406 75 27 particles particle NOUN ct-9406 75 28 , , PUNCT ct-9406 75 29 mostly mostly ADV ct-9406 75 30 sperm sperm NOUN ct-9406 75 31 , , PUNCT ct-9406 75 32 are be AUX ct-9406 75 33 stained stain VERB ct-9406 75 34 . . PUNCT ct-9406 76 1 unstained unstaine VERB ct-9406 76 2 particles particle NOUN ct-9406 76 3 ( ( PUNCT ct-9406 76 4 debris debris NOUN ct-9406 76 5 ) ) PUNCT ct-9406 76 6 are be AUX ct-9406 76 7 removed remove VERB ct-9406 76 8 from from ADP ct-9406 76 9 the the DET ct-9406 76 10 analysis analysis NOUN ct-9406 76 11 . . PUNCT ct-9406 77 1 ( ( PUNCT ct-9406 77 2 c c X ct-9406 77 3 ) ) PUNCT ct-9406 77 4 combination combination NOUN ct-9406 77 5 of of ADP ct-9406 77 6 h33342 h33342 PROPN ct-9406 77 7 and and CCONJ ct-9406 77 8 cellevent cellevent NOUN ct-9406 77 9 ® ® PROPN ct-9406 77 10 ; ; PUNCT ct-9406 77 11 this this DET ct-9406 77 12 combination combination NOUN ct-9406 77 13 of of ADP ct-9406 77 14 probes probe NOUN ct-9406 77 15 identifies identify VERB ct-9406 77 16 3 3 NUM ct-9406 77 17 sperm sperm NOUN ct-9406 77 18 populations population NOUN ct-9406 77 19 corresponding correspond VERB ct-9406 77 20 to to PART ct-9406 77 21 live live VERB ct-9406 77 22 , , PUNCT ct-9406 77 23 apoptotic apoptotic ADJ ct-9406 77 24 , , PUNCT ct-9406 77 25 and and CCONJ ct-9406 77 26 necrotic necrotic ADJ ct-9406 77 27 sperm sperm NOUN ct-9406 77 28 . . PUNCT ct-9406 78 1 ( ( PUNCT ct-9406 78 2 d d X ct-9406 78 3 ) ) PUNCT ct-9406 78 4 double double ADJ ct-9406 78 5 staining stain VERB ct-9406 78 6 with with ADP ct-9406 78 7 3 3 NUM ct-9406 78 8 probes probe NOUN ct-9406 78 9 to to PART ct-9406 78 10 identify identify VERB ct-9406 78 11 apoptotic apoptotic ADJ ct-9406 78 12 changes change NOUN ct-9406 78 13 , , PUNCT ct-9406 78 14 cellevent cellevent NOUN ct-9406 78 15 ® ® PROPN ct-9406 78 16 , , PUNCT ct-9406 78 17 to to PART ct-9406 78 18 identify identify VERB ct-9406 78 19 caspase caspase NOUN ct-9406 78 20 3 3 NUM ct-9406 78 21 positive positive ADJ ct-9406 78 22 sperm sperm NOUN ct-9406 78 23 and and CCONJ ct-9406 78 24 annexin annexin NOUN ct-9406 78 25 - - PUNCT ct-9406 78 26 v v NOUN ct-9406 78 27 to to PART ct-9406 78 28 identify identify VERB ct-9406 78 29 sperm sperm NOUN ct-9406 78 30 having have VERB ct-9406 78 31 transposition transposition NOUN ct-9406 78 32 of of ADP ct-9406 78 33 phosphatidylserine phosphatidylserine NOUN ct-9406 78 34 to to ADP ct-9406 78 35 the the DET ct-9406 78 36 outer outer ADJ ct-9406 78 37 leaflet leaflet NOUN ct-9406 78 38 of of ADP ct-9406 78 39 the the DET ct-9406 78 40 membrane membrane NOUN ct-9406 78 41 . . PUNCT ct-9406 79 1 three three NUM ct-9406 79 2 populations population NOUN ct-9406 79 3 are be AUX ct-9406 79 4 easily easily ADV ct-9406 79 5 identified identify VERB ct-9406 79 6 . . PUNCT ct-9406 80 1 ( ( PUNCT ct-9406 80 2 e e NOUN ct-9406 80 3 ) ) PUNCT ct-9406 80 4 combination combination NOUN ct-9406 80 5 of of ADP ct-9406 80 6 cellevent cellevent NOUN ct-9406 80 7 ® ® PROPN ct-9406 80 8 , , PUNCT ct-9406 80 9 and and CCONJ ct-9406 80 10 the the DET ct-9406 80 11 fixable fixable ADJ ct-9406 80 12 live live ADJ ct-9406 80 13 / / SYM ct-9406 80 14 dead dead ADJ ct-9406 80 15 probe probe NOUN ct-9406 80 16 viakrom viakrom NOUN ct-9406 80 17 808 808 NUM ct-9406 80 18 ® ® NOUN ct-9406 80 19 . . PUNCT ct-9406 81 1 this this DET ct-9406 81 2 combination combination NOUN ct-9406 81 3 easily easily ADV ct-9406 81 4 identifies identify VERB ct-9406 81 5 3 3 NUM ct-9406 81 6 populations population NOUN ct-9406 81 7 : : PUNCT ct-9406 81 8 live live ADJ ct-9406 81 9 , , PUNCT ct-9406 81 10 apoptotic apoptotic ADJ ct-9406 81 11 , , PUNCT ct-9406 81 12 and and CCONJ ct-9406 81 13 necrotic necrotic ADJ ct-9406 81 14 sperm sperm NOUN ct-9406 81 15 . . PUNCT ct-9406 82 1 ( ( PUNCT ct-9406 82 2 f f X ct-9406 82 3 ) ) PUNCT ct-9406 82 4 combination combination NOUN ct-9406 82 5 of of ADP ct-9406 82 6 h33342 h33342 PROPN ct-9406 82 7 and and CCONJ ct-9406 82 8 the the DET ct-9406 82 9 fixable fixable ADJ ct-9406 82 10 live live ADJ ct-9406 82 11 / / SYM ct-9406 82 12 dead dead ADJ ct-9406 82 13 probe probe NOUN ct-9406 82 14 viakrom viakrom NOUN ct-9406 82 15 808 808 NUM ct-9406 82 16 ® ® NOUN ct-9406 82 17 ; ; PUNCT ct-9406 82 18 this this DET ct-9406 82 19 combination combination NOUN ct-9406 82 20 of of ADP ct-9406 82 21 probes probe NOUN ct-9406 82 22 identifies identify VERB ct-9406 82 23 3 3 NUM ct-9406 82 24 types type NOUN ct-9406 82 25 of of ADP ct-9406 82 26 populations population NOUN ct-9406 82 27 : : PUNCT ct-9406 82 28 live live ADJ ct-9406 82 29 sperm sperm NOUN ct-9406 82 30 , , PUNCT ct-9406 82 31 dead dead ADJ ct-9406 82 32 sperm sperm NOUN ct-9406 82 33 , , PUNCT ct-9406 82 34 and and CCONJ ct-9406 82 35 damaged damaged ADJ ct-9406 82 36 sperm sperm NOUN ct-9406 82 37 ( ( PUNCT ct-9406 82 38 2 2 NUM ct-9406 82 39 & & CCONJ ct-9406 82 40 3 3 NUM ct-9406 82 41 positive positive ADJ ct-9406 82 42 for for ADP ct-9406 82 43 viakrom viakrom NOUN ct-9406 82 44 808 808 NUM ct-9406 82 45 ® ® NOUN ct-9406 82 46 ) ) PUNCT ct-9406 82 47 . . PUNCT ct-9406 83 1 a a DET ct-9406 83 2 b b X ct-9406 83 3 c c NOUN ct-9406 83 4 d d X ct-9406 83 5 e e X ct-9406 83 6 f f PROPN ct-9406 83 7 debris debris VERB ct-9406 83 8 c c PROPN ct-9406 83 9 as as ADP ct-9406 83 10 p p PROPN ct-9406 83 11 3 3 NUM ct-9406 83 12 l l NOUN ct-9406 83 13 / / SYM ct-9406 83 14 d d NOUN ct-9406 83 15 viakrom viakrom NOUN ct-9406 83 16 808 808 NUM ct-9406 83 17 sperm sperm NOUN ct-9406 83 18 doublets doublet NOUN ct-9406 83 19 and and CCONJ ct-9406 83 20 clumps clump NOUN ct-9406 83 21 sperm sperm VERB ct-9406 83 22 live live VERB ct-9406 83 23 apoptotic apoptotic ADJ ct-9406 83 24 necrotic necrotic ADJ ct-9406 83 25 annexin annexin NOUN ct-9406 83 26 - - PUNCT ct-9406 83 27 v v NOUN ct-9406 83 28 neg neg NOUN ct-9406 83 29 caspase caspase NOUN ct-9406 83 30 3 3 NUM ct-9406 83 31 neg neg VERB ct-9406 83 32 caspase caspase NOUN ct-9406 83 33 3 3 NUM ct-9406 84 1 + + CCONJ ct-9406 84 2 annexin annexin NOUN ct-9406 84 3 - - PUNCT ct-9406 84 4 v v NOUN ct-9406 84 5 + + CCONJ ct-9406 84 6 caspase caspase NOUN ct-9406 84 7 3 3 NUM ct-9406 84 8 + + PROPN ct-9406 84 9 n n CCONJ ct-9406 84 10 ec ec PROPN ct-9406 84 11 ro ro X ct-9406 84 12 tic tic PROPN ct-9406 84 13 live live VERB ct-9406 84 14 dead dead ADJ ct-9406 84 15 s s PART ct-9406 84 16 s s NOUN ct-9406 84 17 c c NOUN ct-9406 84 18 -h -h SYM ct-9406 85 1 f f X ct-9406 85 2 s s PROPN ct-9406 85 3 c c X ct-9406 85 4 -a -a X ct-9406 85 5 fsc fsc PROPN ct-9406 85 6 - - PUNCT ct-9406 85 7 a a PROPN ct-9406 85 8 h33342 h33342 PROPN ct-9406 85 9 c c PROPN ct-9406 85 10 as as ADP ct-9406 85 11 p p PROPN ct-9406 85 12 3 3 NUM ct-9406 85 13 a a DET ct-9406 85 14 nn nn X ct-9406 85 15 ex ex X ct-9406 85 16 in in ADP ct-9406 85 17 -v -v X ct-9406 85 18 h33342 h33342 PROPN ct-9406 85 19 casp casp NOUN ct-9406 85 20 3 3 NUM ct-9406 85 21 l l NOUN ct-9406 85 22 / / SYM ct-9406 85 23 d d NOUN ct-9406 85 24 viakrom viakrom NOUN ct-9406 85 25 808 808 NUM ct-9406 85 26 h h NOUN ct-9406 85 27 33 33 NUM ct-9406 85 28 34 34 NUM ct-9406 85 29 2 2 NUM ct-9406 85 30 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 NOUN ct-9406 85 31 citation citation NOUN ct-9406 85 32 line line NOUN ct-9406 85 33 : : PUNCT ct-9406 85 34 clinical clinical ADJ ct-9406 85 35 theriogenology theriogenology NOUN ct-9406 85 36 2023 2023 NUM ct-9406 85 37 , , PUNCT ct-9406 85 38 15 15 NUM ct-9406 85 39 , , PUNCT ct-9406 85 40 9406 9406 NUM ct-9406 85 41 , , PUNCT ct-9406 85 42 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 VERB ct-9406 85 43 5 5 NUM ct-9406 85 44 figure figure NOUN ct-9406 85 45 2 2 NUM ct-9406 85 46 . . PUNCT ct-9406 85 47 use use NOUN ct-9406 85 48 of of ADP ct-9406 85 49 artificial artificial ADJ ct-9406 85 50 intelligence intelligence NOUN ct-9406 85 51 techniques technique NOUN ct-9406 85 52 to to PART ct-9406 85 53 simplify simplify VERB ct-9406 85 54 the the DET ct-9406 85 55 output output NOUN ct-9406 85 56 of of ADP ct-9406 85 57 flow flow NOUN ct-9406 85 58 cytometry cytometry NOUN ct-9406 85 59 analysis analysis NOUN ct-9406 85 60 . . PUNCT ct-9406 86 1 figure figure NOUN ct-9406 86 2 presents present VERB ct-9406 86 3 a a DET ct-9406 86 4 flo flo ADJ ct-9406 86 5 self self NOUN ct-9406 86 6 - - PUNCT ct-9406 86 7 organizing organize VERB ct-9406 86 8 mapping mapping NOUN ct-9406 86 9 for for ADP ct-9406 86 10 visualization visualization NOUN ct-9406 86 11 and and CCONJ ct-9406 86 12 organization organization NOUN ct-9406 86 13 of of ADP ct-9406 86 14 flow flow NOUN ct-9406 86 15 cytometry cytometry NOUN ct-9406 86 16 data datum NOUN ct-9406 86 17 where where SCONJ ct-9406 86 18 all all DET ct-9406 86 19 the the DET ct-9406 86 20 characteristics characteristic NOUN ct-9406 86 21 of of ADP ct-9406 86 22 stallion stallion NOUN ct-9406 86 23 sperm sperm NOUN ct-9406 86 24 in in ADP ct-9406 86 25 aliquots aliquot NOUN ct-9406 86 26 of of ADP ct-9406 86 27 the the DET ct-9406 86 28 same same ADJ ct-9406 86 29 ejaculate ejaculate NOUN ct-9406 86 30 , , PUNCT ct-9406 86 31 extended extend VERB ct-9406 86 32 , , PUNCT ct-9406 86 33 and and CCONJ ct-9406 86 34 incubated incubate VERB ct-9406 86 35 at at ADP ct-9406 86 36 37ºc 37ºc NOUN ct-9406 86 37 for for ADP ct-9406 86 38 3 3 NUM ct-9406 86 39 hours hour NOUN ct-9406 86 40 in in ADP ct-9406 86 41 2 2 NUM ct-9406 86 42 media medium NOUN ct-9406 86 43 ( ( PUNCT ct-9406 86 44 1 1 NUM ct-9406 86 45 versus versus ADP ct-9406 86 46 2 2 NUM ct-9406 86 47 ) ) PUNCT ct-9406 86 48 are be AUX ct-9406 86 49 presented present VERB ct-9406 86 50 and and CCONJ ct-9406 86 51 visualized visualize VERB ct-9406 86 52 at at ADP ct-9406 86 53 a a DET ct-9406 86 54 glance glance NOUN ct-9406 86 55 . . PUNCT ct-9406 87 1 here here ADV ct-9406 87 2 , , PUNCT ct-9406 87 3 sperm sperm NOUN ct-9406 87 4 were be AUX ct-9406 87 5 stained stain VERB ct-9406 87 6 with with ADP ct-9406 87 7 a a DET ct-9406 87 8 4 4 NUM ct-9406 87 9 colors color NOUN ct-9406 87 10 protocol protocol NOUN ct-9406 87 11 to to PART ct-9406 87 12 identify identify VERB ct-9406 87 13 live live ADV ct-9406 87 14 , , PUNCT ct-9406 87 15 apoptotic apoptotic ADJ ct-9406 87 16 ( ( PUNCT ct-9406 87 17 caspase caspase NOUN ct-9406 87 18 3 3 NUM ct-9406 87 19 and and CCONJ ct-9406 87 20 annexin annexin NOUN ct-9406 87 21 v v NOUN ct-9406 87 22 ) ) PUNCT ct-9406 87 23 , , PUNCT ct-9406 87 24 and and CCONJ ct-9406 87 25 necrotic necrotic ADJ ct-9406 87 26 sperm sperm NOUN ct-9406 87 27 ( ( PUNCT ct-9406 87 28 live/ live/ NOUN ct-9406 87 29 dead dead ADJ ct-9406 87 30 viakrom viakrom NOUN ct-9406 87 31 808 808 NUM ct-9406 87 32 ® ® NOUN ct-9406 87 33 ) ) PUNCT ct-9406 87 34 in in ADP ct-9406 87 35 particular particular ADJ ct-9406 87 36 clusters cluster NOUN ct-9406 87 37 . . PUNCT ct-9406 88 1 the the DET ct-9406 88 2 gains gain NOUN ct-9406 88 3 in in ADP ct-9406 88 4 time time NOUN ct-9406 88 5 are be AUX ct-9406 88 6 relevant relevant ADJ ct-9406 88 7 , , PUNCT ct-9406 88 8 for for ADP ct-9406 88 9 instance instance NOUN ct-9406 88 10 , , PUNCT ct-9406 88 11 after after ADP ct-9406 88 12 live live ADJ ct-9406 88 13 sperm sperm NOUN ct-9406 88 14 ( ( PUNCT ct-9406 88 15 red red ADJ ct-9406 88 16 circles circle NOUN ct-9406 88 17 ) ) PUNCT ct-9406 88 18 comparing compare VERB ct-9406 88 19 the the DET ct-9406 88 20 2 2 NUM ct-9406 88 21 incubation incubation NOUN ct-9406 88 22 panels panel NOUN ct-9406 88 23 ( ( PUNCT ct-9406 88 24 1 1 NUM ct-9406 88 25 versus versus ADP ct-9406 88 26 2 2 NUM ct-9406 88 27 ) ) PUNCT ct-9406 88 28 . . PUNCT ct-9406 89 1 in in ADP ct-9406 89 2 1a 1a NUM ct-9406 89 3 , , PUNCT ct-9406 89 4 there there PRON ct-9406 89 5 are be VERB ct-9406 89 6 more more ADV ct-9406 89 7 viable viable ADJ ct-9406 89 8 sperm sperm NOUN ct-9406 89 9 than than ADP ct-9406 89 10 in in ADP ct-9406 89 11 panel panel NOUN ct-9406 89 12 2 2 NUM ct-9406 89 13 , , PUNCT ct-9406 89 14 where where SCONJ ct-9406 89 15 there there PRON ct-9406 89 16 is be VERB ct-9406 89 17 a a DET ct-9406 89 18 larger large ADJ ct-9406 89 19 abundance abundance NOUN ct-9406 89 20 of of ADP ct-9406 89 21 sperm sperm NOUN ct-9406 89 22 having have VERB ct-9406 89 23 several several ADJ ct-9406 89 24 apoptotic apoptotic ADJ ct-9406 89 25 markers marker NOUN ct-9406 89 26 . . PUNCT ct-9406 90 1 interestingly interestingly ADV ct-9406 90 2 as as ADV ct-9406 90 3 well well ADV ct-9406 90 4 , , PUNCT ct-9406 90 5 panel panel NOUN ct-9406 90 6 2 2 NUM ct-9406 90 7 depicts depict VERB ct-9406 90 8 the the DET ct-9406 90 9 presence presence NOUN ct-9406 90 10 of of ADP ct-9406 90 11 many many ADJ ct-9406 90 12 live live ADJ ct-9406 90 13 sperm sperm NOUN ct-9406 90 14 having have VERB ct-9406 90 15 phosphatidylserine phosphatidylserine NOUN ct-9406 90 16 transposition transposition NOUN ct-9406 90 17 to to ADP ct-9406 90 18 the the DET ct-9406 90 19 outer outer ADJ ct-9406 90 20 membrane membrane NOUN ct-9406 90 21 , , PUNCT ct-9406 90 22 representing represent VERB ct-9406 90 23 unstable unstable ADJ ct-9406 90 24 membranes membrane NOUN ct-9406 90 25 . . PUNCT ct-9406 91 1 anexin+ anexin+ PROPN ct-9406 91 2 apoptotic apoptotic ADJ ct-9406 91 3 necrotic necrotic ADJ ct-9406 91 4 spermatozoa spermatozoa NOUN ct-9406 91 5 viable viable ADJ ct-9406 91 6 1 1 NUM ct-9406 91 7 metacluster metacluster NOUN ct-9406 91 8 1 1 NUM ct-9406 91 9 2 2 NUM ct-9406 91 10 3 3 NUM ct-9406 91 11 4 4 NUM ct-9406 91 12 5 5 NUM ct-9406 91 13 6 6 NUM ct-9406 91 14 7 7 NUM ct-9406 91 15 8 8 NUM ct-9406 91 16 9 9 NUM ct-9406 91 17 10 10 NUM ct-9406 91 18 a a DET ct-9406 91 19 b b NOUN ct-9406 91 20 anexin+ anexin+ ADP ct-9406 91 21 apoptotic apoptotic ADJ ct-9406 91 22 necrotic necrotic ADJ ct-9406 91 23 spermatozoa spermatozoa NOUN ct-9406 91 24 viable viable ADJ ct-9406 91 25 c c NOUN ct-9406 91 26 d d SYM ct-9406 91 27 2 2 NUM ct-9406 91 28 metacluster metacluster NOUN ct-9406 91 29 1 1 NUM ct-9406 91 30 2 2 NUM ct-9406 91 31 3 3 NUM ct-9406 91 32 4 4 NUM ct-9406 91 33 5 5 NUM ct-9406 91 34 6 6 NUM ct-9406 91 35 7 7 NUM ct-9406 91 36 8 8 NUM ct-9406 91 37 9 9 NUM ct-9406 91 38 10 10 NUM ct-9406 91 39 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 NOUN ct-9406 91 40 6 6 NUM ct-9406 91 41 citation citation NOUN ct-9406 91 42 line line NOUN ct-9406 91 43 : : PUNCT ct-9406 91 44 clinical clinical ADJ ct-9406 91 45 theriogenology theriogenology NOUN ct-9406 91 46 2023 2023 NUM ct-9406 91 47 , , PUNCT ct-9406 91 48 15 15 NUM ct-9406 91 49 , , PUNCT ct-9406 91 50 9406 9406 NUM ct-9406 91 51 , , PUNCT ct-9406 91 52 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 NOUN ct-9406 91 53 latter latter ADJ ct-9406 91 54 , , PUNCT ct-9406 91 55 sperm sperm NOUN ct-9406 91 56 having have VERB ct-9406 91 57 various various ADJ ct-9406 91 58 apoptotic apoptotic ADJ ct-9406 91 59 markers marker NOUN ct-9406 91 60 are be AUX ct-9406 91 61 more more ADV ct-9406 91 62 abundant abundant ADJ ct-9406 91 63 ( ( PUNCT ct-9406 91 64 see see NOUN ct-9406 91 65 2d 2d NOUN ct-9406 91 66 ) ) PUNCT ct-9406 91 67 . . PUNCT ct-9406 92 1 moreover moreover ADV ct-9406 92 2 , , PUNCT ct-9406 92 3 many many ADJ ct-9406 92 4 live live ADJ ct-9406 92 5 sperm sperm NOUN ct-9406 92 6 exhibit exhibit NOUN ct-9406 92 7 phosphatidylserine phosphatidylserine NOUN ct-9406 92 8 transposition transposition NOUN ct-9406 92 9 to to ADP ct-9406 92 10 the the DET ct-9406 92 11 outer outer ADJ ct-9406 92 12 membrane membrane NOUN ct-9406 92 13 , , PUNCT ct-9406 92 14 representing represent VERB ct-9406 92 15 unstable unstable ADJ ct-9406 92 16 membranes membrane NOUN ct-9406 92 17 . . PUNCT ct-9406 93 1 such such ADJ ct-9406 93 2 sperm sperm NOUN ct-9406 93 3 are be AUX ct-9406 93 4 at at ADP ct-9406 93 5 risk risk NOUN ct-9406 93 6 , , PUNCT ct-9406 93 7 and and CCONJ ct-9406 93 8 the the DET ct-9406 93 9 clinician clinician NOUN ct-9406 93 10 benefits benefit NOUN ct-9406 93 11 from from ADP ct-9406 93 12 knowing know VERB ct-9406 93 13 all all DET ct-9406 93 14 this this DET ct-9406 93 15 battery battery NOUN ct-9406 93 16 of of ADP ct-9406 93 17 data datum NOUN ct-9406 93 18 and and CCONJ ct-9406 93 19 its its PRON ct-9406 93 20 quantification quantification NOUN ct-9406 93 21 before before ADP ct-9406 93 22 deciding decide VERB ct-9406 93 23 the the DET ct-9406 93 24 fate fate NOUN ct-9406 93 25 of of ADP ct-9406 93 26 the the DET ct-9406 93 27 ejaculate ejaculate NOUN ct-9406 93 28 or or CCONJ ct-9406 93 29 the the DET ct-9406 93 30 use use NOUN ct-9406 93 31 of of ADP ct-9406 93 32 a a DET ct-9406 93 33 processed process VERB ct-9406 93 34 sample sample NOUN ct-9406 93 35 for for ADP ct-9406 93 36 further further ADJ ct-9406 93 37 use use NOUN ct-9406 93 38 . . PUNCT ct-9406 94 1 hopefully hopefully ADV ct-9406 94 2 , , PUNCT ct-9406 94 3 there there PRON ct-9406 94 4 shall shall AUX ct-9406 94 5 be be AUX ct-9406 94 6 gains gain NOUN ct-9406 94 7 in in ADP ct-9406 94 8 diagnostics diagnostic NOUN ct-9406 94 9 and and CCONJ ct-9406 94 10 in in ADP ct-9406 94 11 resulting result VERB ct-9406 94 12 fertility fertility NOUN ct-9406 94 13 . . PUNCT ct-9406 95 1 concluding conclude VERB ct-9406 95 2 remark remark NOUN ct-9406 95 3 cytomics cytomic NOUN ct-9406 95 4 , , PUNCT ct-9406 95 5 combining combine VERB ct-9406 95 6 novel novel ADJ ct-9406 95 7 designed design VERB ct-9406 95 8 motility motility NOUN ct-9406 95 9 analyzers analyzer NOUN ct-9406 95 10 , , PUNCT ct-9406 95 11 multiparametric multiparametric ADJ ct-9406 95 12 flow flow NOUN ct-9406 95 13 cytometers cytometer NOUN ct-9406 95 14 , , PUNCT ct-9406 95 15 and and CCONJ ct-9406 95 16 enhanced enhance VERB ct-9406 95 17 digital digital ADJ ct-9406 95 18 imaging imaging NOUN ct-9406 95 19 together together ADV ct-9406 95 20 with with ADP ct-9406 95 21 the the DET ct-9406 95 22 incorporation incorporation NOUN ct-9406 95 23 of of ADP ct-9406 95 24 artificial artificial ADJ ct-9406 95 25 intelligence intelligence NOUN ct-9406 95 26 in in ADP ct-9406 95 27 the the DET ct-9406 95 28 analysis analysis NOUN ct-9406 95 29 , , PUNCT ct-9406 95 30 shall shall AUX ct-9406 95 31 dominate dominate VERB ct-9406 95 32 the the DET ct-9406 95 33 landscape landscape NOUN ct-9406 95 34 of of ADP ct-9406 95 35 andrological andrological ADJ ct-9406 95 36 laboratories laboratory NOUN ct-9406 95 37 and and CCONJ ct-9406 95 38 enable enable VERB ct-9406 95 39 quick quick ADJ ct-9406 95 40 determinations determination NOUN ct-9406 95 41 on on ADP ct-9406 95 42 huge huge ADJ ct-9406 95 43 sperm sperm NOUN ct-9406 95 44 numbers number NOUN ct-9406 95 45 for for ADP ct-9406 95 46 markers marker NOUN ct-9406 95 47 highly highly ADV ct-9406 95 48 relevant relevant ADJ ct-9406 95 49 to to AUX ct-9406 95 50 sperm sperm NOUN ct-9406 95 51 function function NOUN ct-9406 95 52 and and CCONJ ct-9406 95 53 , , PUNCT ct-9406 95 54 hence hence ADV ct-9406 95 55 , , PUNCT ct-9406 95 56 for for ADP ct-9406 95 57 fertility fertility NOUN ct-9406 95 58 . . PUNCT ct-9406 96 1 such such ADJ ct-9406 96 2 advancements advancement NOUN ct-9406 96 3 shall shall AUX ct-9406 96 4 , , PUNCT ct-9406 96 5 however however ADV ct-9406 96 6 , , PUNCT ct-9406 96 7 hardly hardly ADV ct-9406 96 8 replace replace VERB ct-9406 96 9 essential essential ADJ ct-9406 96 10 clinical clinical ADJ ct-9406 96 11 andrological andrological ADJ ct-9406 96 12 practice practice NOUN ct-9406 96 13 . . PUNCT ct-9406 97 1 ethical ethical ADJ ct-9406 97 2 permission permission NOUN ct-9406 97 3 housing housing NOUN ct-9406 97 4 , , PUNCT ct-9406 97 5 handling handling NOUN ct-9406 97 6 , , PUNCT ct-9406 97 7 and and CCONJ ct-9406 97 8 collection collection NOUN ct-9406 97 9 of of ADP ct-9406 97 10 semen seman NOUN ct-9406 97 11 that that PRON ct-9406 97 12 have have AUX ct-9406 97 13 been be AUX ct-9406 97 14 used use VERB ct-9406 97 15 during during ADP ct-9406 97 16 the the DET ct-9406 97 17 analyses analysis NOUN ct-9406 97 18 described describe VERB ct-9406 97 19 in in ADP ct-9406 97 20 this this DET ct-9406 97 21 review review NOUN ct-9406 97 22 have have AUX ct-9406 97 23 followed follow VERB ct-9406 97 24 the the DET ct-9406 97 25 european european ADJ ct-9406 97 26 community community NOUN ct-9406 97 27 directive directive NOUN ct-9406 97 28 2010/63 2010/63 PROPN ct-9406 97 29 / / SYM ct-9406 97 30 eu eu PROPN ct-9406 97 31 , , PUNCT ct-9406 97 32 22/09/2010 22/09/2010 NUM ct-9406 97 33 , , PUNCT ct-9406 97 34 and and CCONJ ct-9406 97 35 current current ADJ ct-9406 97 36 spanish spanish ADJ ct-9406 97 37 ( ( PUNCT ct-9406 97 38 law law NOUN ct-9406 97 39 6/2913 6/2913 NUM ct-9406 97 40 june june PROPN ct-9406 97 41 11th 11th NOUN ct-9406 97 42 and and CCONJ ct-9406 97 43 european european ADJ ct-9406 97 44 directive directive NOUN ct-9406 97 45 2010/63 2010/63 PROPN ct-9406 97 46 / / SYM ct-9406 97 47 eu eu NOUN ct-9406 97 48 ) ) PUNCT ct-9406 97 49 and and CCONJ ct-9406 97 50 swedish swedish ADJ ct-9406 97 51 legislation legislation NOUN ct-9406 97 52 ( ( PUNCT ct-9406 97 53 sjvfs sjvfs NOUN ct-9406 97 54 2017:40 2017:40 NUM ct-9406 97 55 ) ) PUNCT ct-9406 97 56 . . PUNCT ct-9406 98 1 studies study NOUN ct-9406 98 2 involved involve VERB ct-9406 98 3 had have AUX ct-9406 98 4 previously previously ADV ct-9406 98 5 been be AUX ct-9406 98 6 scrutinized scrutinize VERB ct-9406 98 7 and and CCONJ ct-9406 98 8 approved approve VERB ct-9406 98 9 by by ADP ct-9406 98 10 the the DET ct-9406 98 11 ethical ethical ADJ ct-9406 98 12 committee committee NOUN ct-9406 98 13 of of ADP ct-9406 98 14 the the DET ct-9406 98 15 university university PROPN ct-9406 98 16 of of ADP ct-9406 98 17 extremadura extremadura PROPN ct-9406 98 18 ( ( PUNCT ct-9406 98 19 pid2021 pid2021 NOUN ct-9406 98 20 - - PUNCT ct-9406 98 21 122351ob 122351ob NOUN ct-9406 98 22 - - PUNCT ct-9406 98 23 i00 i00 NOUN ct-9406 98 24 and and CCONJ ct-9406 98 25 ib20008 ib20008 NOUN ct-9406 98 26 ) ) PUNCT ct-9406 98 27 and and CCONJ ct-9406 98 28 the the DET ct-9406 98 29 swedish swedish PROPN ct-9406 98 30 regional regional PROPN ct-9406 98 31 committee committee PROPN ct-9406 98 32 for for ADP ct-9406 98 33 ethical ethical ADJ ct-9406 98 34 approval approval NOUN ct-9406 98 35 of of ADP ct-9406 98 36 animal animal NOUN ct-9406 98 37 experiments experiment NOUN ct-9406 98 38 ( ( PUNCT ct-9406 98 39 linköpings linköping NOUN ct-9406 98 40 djurförsöksetiska djurförsöksetiska VERB ct-9406 98 41 nämnd nämnd NOUN ct-9406 98 42 , , PUNCT ct-9406 98 43 linköping linköpe VERB ct-9406 98 44 , , PUNCT ct-9406 98 45 sweden sweden PROPN ct-9406 98 46 , , PUNCT ct-9406 98 47 permit permit NOUN ct-9406 98 48 numbers number NOUN ct-9406 98 49 75 75 NUM ct-9406 98 50 - - SYM ct-9406 98 51 12 12 NUM ct-9406 98 52 ( ( PUNCT ct-9406 98 53 10/02/2012 10/02/2012 NUM ct-9406 98 54 ) ) PUNCT ct-9406 98 55 , , PUNCT ct-9406 98 56 id1400 id1400 NOUN ct-9406 98 57 ( ( PUNCT ct-9406 98 58 02/02/2018 02/02/2018 NUM ct-9406 98 59 ) ) PUNCT ct-9406 98 60 , , PUNCT ct-9406 98 61 and and CCONJ ct-9406 98 62 dnr dnr PROPN ct-9406 98 63 03416 03416 NUM ct-9406 98 64 - - SYM ct-9406 98 65 2020 2020 NUM ct-9406 98 66 ( ( PUNCT ct-9406 98 67 26/03/2020 26/03/2020 NUM ct-9406 98 68 ) ) PUNCT ct-9406 98 69 ) ) PUNCT ct-9406 98 70 . . PUNCT ct-9406 99 1 conflict conflict NOUN ct-9406 99 2 of of ADP ct-9406 99 3 interest interest NOUN ct-9406 99 4 authors author NOUN ct-9406 99 5 declare declare VERB ct-9406 99 6 no no DET ct-9406 99 7 ( ( PUNCT ct-9406 99 8 financial financial ADJ ct-9406 99 9 , , PUNCT ct-9406 99 10 commercial commercial ADJ ct-9406 99 11 , , PUNCT ct-9406 99 12 or or CCONJ ct-9406 99 13 personal personal ADJ ct-9406 99 14 ) ) PUNCT ct-9406 99 15 conflict conflict NOUN ct-9406 99 16 of of ADP ct-9406 99 17 interest interest NOUN ct-9406 99 18 . . PUNCT ct-9406 100 1 author author NOUN ct-9406 100 2 contribution contribution NOUN ct-9406 100 3 authors author NOUN ct-9406 100 4 contributed contribute VERB ct-9406 100 5 equally equally ADV ct-9406 100 6 to to ADP ct-9406 100 7 the the DET ct-9406 100 8 design design NOUN ct-9406 100 9 and and CCONJ ct-9406 100 10 writing writing NOUN ct-9406 100 11 . . PUNCT ct-9406 101 1 acknowledgments acknowledgment NOUN ct-9406 101 2 authors author NOUN ct-9406 101 3 thank thank VERB ct-9406 101 4 former former ADJ ct-9406 101 5 and and CCONJ ct-9406 101 6 present present ADJ ct-9406 101 7 students student NOUN ct-9406 101 8 , , PUNCT ct-9406 101 9 post post NOUN ct-9406 101 10 - - ADJ ct-9406 101 11 docs doc NOUN ct-9406 101 12 , , PUNCT ct-9406 101 13 and and CCONJ ct-9406 101 14 collaborators collaborator NOUN ct-9406 101 15 engaged engage VERB ct-9406 101 16 in in ADP ct-9406 101 17 many many ADJ ct-9406 101 18 of of ADP ct-9406 101 19 the the DET ct-9406 101 20 studies study NOUN ct-9406 101 21 reviewed review VERB ct-9406 101 22 . . PUNCT ct-9406 102 1 funding fund VERB ct-9406 102 2 these these DET ct-9406 102 3 studies study NOUN ct-9406 102 4 were be AUX ct-9406 102 5 made make VERB ct-9406 102 6 possible possible ADJ ct-9406 102 7 by by ADP ct-9406 102 8 grants grant NOUN ct-9406 102 9 from from ADP ct-9406 102 10 the the DET ct-9406 102 11 ministry ministry PROPN ct-9406 102 12 of of ADP ct-9406 102 13 science science PROPN ct-9406 102 14 - - PUNCT ct-9406 102 15 european european PROPN ct-9406 102 16 fund fund NOUN ct-9406 102 17 for for ADP ct-9406 102 18 regional regional ADJ ct-9406 102 19 development development NOUN ct-9406 102 20 ( ( PUNCT ct-9406 102 21 efrd efrd PROPN ct-9406 102 22 ) ) PUNCT ct-9406 102 23 , , PUNCT ct-9406 102 24 madrid madrid PROPN ct-9406 102 25 , , PUNCT ct-9406 102 26 spain spain PROPN ct-9406 102 27 ( ( PUNCT ct-9406 102 28 grant grant VERB ct-9406 102 29 pid2021 pid2021 NOUN ct-9406 102 30 - - PUNCT ct-9406 102 31 122351ob 122351ob NOUN ct-9406 102 32 - - PUNCT ct-9406 102 33 i00 i00 NOUN ct-9406 102 34 ) ) PUNCT ct-9406 102 35 ; ; PUNCT ct-9406 102 36 junta junta PROPN ct-9406 102 37 de de X ct-9406 102 38 extremadura extremadura PROPN ct-9406 102 39 - - PUNCT ct-9406 102 40 feder feder PROPN ct-9406 102 41 ( ( PUNCT ct-9406 102 42 gr gr PROPN ct-9406 102 43 21060 21060 NUM ct-9406 102 44 and and CCONJ ct-9406 102 45 ib ib PROPN ct-9406 102 46 20008 20008 NUM ct-9406 102 47 ) ) PUNCT ct-9406 102 48 , , PUNCT ct-9406 102 49 spain spain PROPN ct-9406 102 50 ; ; PUNCT ct-9406 102 51 the the DET ct-9406 102 52 swedish swedish PROPN ct-9406 102 53 research research PROPN ct-9406 102 54 council council PROPN ct-9406 102 55 formas forma NOUN ct-9406 102 56 ; ; PUNCT ct-9406 102 57 and and CCONJ ct-9406 103 1 the the DET ct-9406 103 2 swedish swedish PROPN ct-9406 103 3 research research PROPN ct-9406 103 4 council council PROPN ct-9406 103 5 ( ( PUNCT ct-9406 103 6 vr vr PROPN ct-9406 103 7 ) ) PUNCT ct-9406 103 8 , , PUNCT ct-9406 103 9 stockholm stockholm PROPN ct-9406 103 10 , , PUNCT ct-9406 103 11 sweden sweden PROPN ct-9406 103 12 . . PUNCT ct-9406 103 13 references reference NOUN ct-9406 103 14 1 1 NUM ct-9406 103 15 . . PUNCT ct-9406 104 1 kastelic kastelic PROPN ct-9406 104 2 jp jp PROPN ct-9406 104 3 , , PUNCT ct-9406 104 4 thundathil thundathil PROPN ct-9406 104 5 jc jc PROPN ct-9406 104 6 : : PUNCT ct-9406 104 7 breeding breed VERB ct-9406 104 8 soundness soundness NOUN ct-9406 104 9 evaluation evaluation NOUN ct-9406 104 10 and and CCONJ ct-9406 104 11 semen semen NOUN ct-9406 104 12 analysis analysis NOUN ct-9406 104 13 for for ADP ct-9406 104 14 predicting predict VERB ct-9406 104 15 bull bull NOUN ct-9406 104 16 fertility fertility NOUN ct-9406 104 17 . . PUNCT ct-9406 105 1 reprod reprod PROPN ct-9406 105 2 domest domest PROPN ct-9406 105 3 anim anim PROPN ct-9406 105 4 2008;43 2008;43 NUM ct-9406 105 5 suppl suppl ADJ ct-9406 105 6 2:368–373 2:368–373 PROPN ct-9406 105 7 . . PUNCT ct-9406 106 1 doi doi NOUN ct-9406 106 2 : : PUNCT ct-9406 106 3 10.1111 10.1111 NUM ct-9406 106 4 / / SYM ct-9406 106 5 j.1439 j.1439 PROPN ct-9406 106 6 - - PUNCT ct-9406 106 7 0531 0531 NUM ct-9406 106 8 . . PUNCT ct-9406 107 1 2008.01186.x 2008.01186.x NUM ct-9406 107 2 2 2 NUM ct-9406 107 3 . . PUNCT ct-9406 107 4 rodriguez rodriguez NOUN ct-9406 107 5 - - PUNCT ct-9406 107 6 martinez martinez PROPN ct-9406 107 7 h h PROPN ct-9406 107 8 : : PUNCT ct-9406 107 9 semen semen NOUN ct-9406 107 10 evaluation evaluation NOUN ct-9406 107 11 and and CCONJ ct-9406 107 12 handling handling NOUN ct-9406 107 13 in in ADP ct-9406 107 14 : : PUNCT ct-9406 107 15 pjcasl pjcasl PROPN ct-9406 107 16 , , PUNCT ct-9406 107 17 editor editor NOUN ct-9406 107 18 . . PUNCT ct-9406 108 1 animal animal NOUN ct-9406 108 2 andrology andrology NOUN ct-9406 108 3 : : PUNCT ct-9406 108 4 theory theory NOUN ct-9406 108 5 and and CCONJ ct-9406 108 6 applications application NOUN ct-9406 108 7 . . PUNCT ct-9406 109 1 oxfordshire oxfordshire NOUN ct-9406 109 2 , , PUNCT ct-9406 109 3 uk uk PROPN ct-9406 109 4 ; ; PUNCT ct-9406 109 5 cabi cabi PROPN ct-9406 109 6 : : PUNCT ct-9406 110 1 2014:509–594 2014:509–594 X ct-9406 110 2 . . NOUN ct-9406 111 1 3 3 X ct-9406 111 2 . . X ct-9406 111 3 rodriguez rodriguez NOUN ct-9406 111 4 - - PUNCT ct-9406 111 5 martinez martinez PROPN ct-9406 111 6 h h PROPN ct-9406 111 7 : : PUNCT ct-9406 111 8 state state NOUN ct-9406 111 9 of of ADP ct-9406 111 10 the the DET ct-9406 111 11 art art NOUN ct-9406 111 12 in in ADP ct-9406 111 13 farm farm NOUN ct-9406 111 14 animal animal NOUN ct-9406 111 15 sperm sperm NOUN ct-9406 111 16 evaluation evaluation NOUN ct-9406 111 17 . . PUNCT ct-9406 112 1 reprod reprod PROPN ct-9406 112 2 fertil fertil PROPN ct-9406 112 3 dev dev PROPN ct-9406 112 4 2007;19:91–101 2007;19:91–101 NUM ct-9406 112 5 . . PUNCT ct-9406 113 1 doi doi NOUN ct-9406 113 2 : : PUNCT ct-9406 113 3 10.1071 10.1071 NUM ct-9406 113 4 / / SYM ct-9406 113 5 rd06104 rd06104 NOUN ct-9406 113 6 4 4 NUM ct-9406 113 7 . . PUNCT ct-9406 114 1 rodriguez rodriguez NOUN ct-9406 114 2 - - PUNCT ct-9406 114 3 martinez martinez PROPN ct-9406 114 4 h h PROPN ct-9406 114 5 , , PUNCT ct-9406 114 6 barth barth NOUN ct-9406 114 7 ad ad NOUN ct-9406 114 8 : : PUNCT ct-9406 114 9 in in ADP ct-9406 114 10 vitro vitro X ct-9406 114 11 evaluation evaluation NOUN ct-9406 114 12 of of ADP ct-9406 114 13 sperm sperm NOUN ct-9406 114 14 quality quality NOUN ct-9406 114 15 related relate VERB ct-9406 114 16 to to ADP ct-9406 114 17 in in ADP ct-9406 114 18 vivo vivo ADJ ct-9406 114 19 function function NOUN ct-9406 114 20 and and CCONJ ct-9406 114 21 fertility fertility NOUN ct-9406 114 22 . . PUNCT ct-9406 115 1 soc soc NOUN ct-9406 115 2 reprod reprod PROPN ct-9406 115 3 fertil fertil VERB ct-9406 115 4 suppl suppl ADJ ct-9406 115 5 2007;64:39–54 2007;64:39–54 NUM ct-9406 115 6 . . PUNCT ct-9406 116 1 doi doi NOUN ct-9406 116 2 : : PUNCT ct-9406 116 3 10.5661 10.5661 NUM ct-9406 116 4 / / SYM ct-9406 116 5 rdr rdr NOUN ct-9406 116 6 - - PUNCT ct-9406 116 7 vi-39 vi-39 NOUN ct-9406 116 8 5 5 NUM ct-9406 116 9 . . PUNCT ct-9406 117 1 al al PROPN ct-9406 117 2 - - PUNCT ct-9406 117 3 makhzoomi makhzoomi PROPN ct-9406 117 4 a a NOUN ct-9406 117 5 , , PUNCT ct-9406 117 6 lundeheim lundeheim NOUN ct-9406 117 7 n n CCONJ ct-9406 117 8 , , PUNCT ct-9406 117 9 haard haard PROPN ct-9406 117 10 m m PROPN ct-9406 117 11 , , PUNCT ct-9406 117 12 et et PROPN ct-9406 117 13 al al PROPN ct-9406 117 14 : : PUNCT ct-9406 117 15 sperm sperm NOUN ct-9406 117 16 morphology morphology NOUN ct-9406 117 17 and and CCONJ ct-9406 117 18 fertility fertility NOUN ct-9406 117 19 of of ADP ct-9406 117 20 progeny progeny ADV ct-9406 117 21 - - PUNCT ct-9406 117 22 tested test VERB ct-9406 117 23 ai ai NOUN ct-9406 117 24 dairy dairy NOUN ct-9406 117 25 bulls bull NOUN ct-9406 117 26 in in ADP ct-9406 117 27 sweden sweden PROPN ct-9406 117 28 . . PUNCT ct-9406 118 1 theriogenology theriogenology NOUN ct-9406 118 2 2008;70:682–691 2008;70:682–691 PROPN ct-9406 118 3 . . PUNCT ct-9406 119 1 doi doi NOUN ct-9406 119 2 : : PUNCT ct-9406 119 3 10.1016 10.1016 NUM ct-9406 119 4 / / SYM ct-9406 119 5 j.theriogenology j.theriogenology NOUN ct-9406 119 6 . . PUNCT ct-9406 120 1 2008.04.049 2008.04.049 PRON ct-9406 120 2 6 6 NUM ct-9406 120 3 . . PUNCT ct-9406 120 4 rodrígue rodrígue NOUN ct-9406 120 5 martínez martínez PROPN ct-9406 120 6 h h NOUN ct-9406 120 7 : : PUNCT ct-9406 120 8 semen semen NOUN ct-9406 120 9 evaluation evaluation NOUN ct-9406 120 10 : : PUNCT ct-9406 120 11 can can AUX ct-9406 120 12 we we PRON ct-9406 120 13 forecast forecast VERB ct-9406 120 14 fertility fertility NOUN ct-9406 120 15 ? ? PUNCT ct-9406 121 1 vet vet NOUN ct-9406 121 2 stanica stanica PROPN ct-9406 121 3 2019;50:293–305 2019;50:293–305 PROPN ct-9406 121 4 . . PUNCT ct-9406 122 1 7 7 X ct-9406 122 2 . . X ct-9406 122 3 dejarnette dejarnette PROPN ct-9406 122 4 jm jm PROPN ct-9406 122 5 , , PUNCT ct-9406 122 6 harstine harstine PROPN ct-9406 122 7 br br PROPN ct-9406 122 8 , , PUNCT ct-9406 122 9 mcdonald mcdonald PROPN ct-9406 122 10 k k PROPN ct-9406 122 11 , , PUNCT ct-9406 122 12 et et PROPN ct-9406 122 13 al al PROPN ct-9406 122 14 : : PUNCT ct-9406 122 15 commercial commercial ADJ ct-9406 122 16 application application NOUN ct-9406 122 17 of of ADP ct-9406 122 18 flow flow NOUN ct-9406 122 19 cytometry cytometry NOUN ct-9406 122 20 for for ADP ct-9406 122 21 evaluating evaluate VERB ct-9406 122 22 bull bull NOUN ct-9406 122 23 sperm sperm NOUN ct-9406 122 24 . . PUNCT ct-9406 123 1 anim anim NOUN ct-9406 123 2 reprod reprod PROPN ct-9406 123 3 sci sci PROPN ct-9406 123 4 2022;246:106838 2022;246:106838 NUM ct-9406 123 5 . . PUNCT ct-9406 124 1 doi doi NOUN ct-9406 124 2 : : PUNCT ct-9406 124 3 10.1016 10.1016 NUM ct-9406 124 4 / / SYM ct-9406 124 5 j.anireprosci.2021 j.anireprosci.2021 PROPN ct-9406 124 6 . . PUNCT ct-9406 125 1 106838 106838 NUM ct-9406 125 2 8 8 NUM ct-9406 125 3 . . PUNCT ct-9406 126 1 rodriguez rodriguez NOUN ct-9406 126 2 - - PUNCT ct-9406 126 3 martinez martinez PROPN ct-9406 126 4 h h PROPN ct-9406 126 5 , , PUNCT ct-9406 126 6 kvist kvist NOUN ct-9406 126 7 u u NOUN ct-9406 126 8 , , PUNCT ct-9406 126 9 ernerudh ernerudh NOUN ct-9406 126 10 j j PROPN ct-9406 126 11 , , PUNCT ct-9406 126 12 et et PROPN ct-9406 126 13 al al PROPN ct-9406 126 14 : : PUNCT ct-9406 126 15 seminal seminal ADJ ct-9406 126 16 plasma plasma NOUN ct-9406 126 17 proteins protein NOUN ct-9406 126 18 : : PUNCT ct-9406 126 19 what what DET ct-9406 126 20 role role NOUN ct-9406 126 21 do do AUX ct-9406 126 22 they they PRON ct-9406 126 23 play play VERB ct-9406 126 24 ? ? PUNCT ct-9406 127 1 am be AUX ct-9406 127 2 j j PROPN ct-9406 127 3 reprod reprod PROPN ct-9406 127 4 immunol immunol NOUN ct-9406 127 5 2011;66 2011;66 NUM ct-9406 127 6 suppl suppl ADJ ct-9406 127 7 1:11–22 1:11–22 NUM ct-9406 127 8 . . PUNCT ct-9406 128 1 doi doi NOUN ct-9406 128 2 : : PUNCT ct-9406 128 3 10.1111 10.1111 NUM ct-9406 128 4 / / SYM ct-9406 128 5 j.1600 j.1600 NOUN ct-9406 128 6 - - PUNCT ct-9406 128 7 0897.2011.01033.x 0897.2011.01033.x NOUN ct-9406 128 8 9 9 NUM ct-9406 128 9 . . X ct-9406 129 1 perez perez PROPN ct-9406 129 2 - - PUNCT ct-9406 129 3 patino patino PROPN ct-9406 129 4 c c PROPN ct-9406 129 5 , , PUNCT ct-9406 129 6 parrilla parrilla PROPN ct-9406 129 7 i i PROPN ct-9406 129 8 , , PUNCT ct-9406 129 9 barranco barranco PROPN ct-9406 129 10 i i PROPN ct-9406 129 11 , , PUNCT ct-9406 129 12 et et PROPN ct-9406 129 13 al al PROPN ct-9406 129 14 : : PUNCT ct-9406 129 15 new new ADJ ct-9406 129 16 in in ADP ct-9406 129 17 - - PUNCT ct-9406 129 18 depth depth NOUN ct-9406 129 19 analytical analytical ADJ ct-9406 129 20 approach approach NOUN ct-9406 129 21 of of ADP ct-9406 129 22 the the DET ct-9406 129 23 porcine porcine NOUN ct-9406 129 24 seminal seminal ADJ ct-9406 129 25 plasma plasma NOUN ct-9406 129 26 proteome proteome NOUN ct-9406 129 27 reveals reveal VERB ct-9406 129 28 potential potential ADJ ct-9406 129 29 fertility fertility NOUN ct-9406 129 30 biomarkers biomarker NOUN ct-9406 129 31 . . PUNCT ct-9406 130 1 j j PROPN ct-9406 130 2 proteome proteome PROPN ct-9406 130 3 res re VERB ct-9406 130 4 2018;17:1065–1076 2018;17:1065–1076 PROPN ct-9406 130 5 . . PUNCT ct-9406 131 1 doi doi NOUN ct-9406 131 2 : : PUNCT ct-9406 131 3 10.1021 10.1021 NUM ct-9406 131 4 / / SYM ct-9406 131 5 acs.jproteome.7b00728 acs.jproteome.7b00728 NOUN ct-9406 131 6 10 10 NUM ct-9406 131 7 . . PUNCT ct-9406 132 1 rodriguez rodriguez NOUN ct-9406 132 2 - - PUNCT ct-9406 132 3 martinez martinez PROPN ct-9406 132 4 h h PROPN ct-9406 132 5 , , PUNCT ct-9406 132 6 martinez martinez PROPN ct-9406 132 7 ea ea PROPN ct-9406 132 8 , , PUNCT ct-9406 132 9 calvete calvete PROPN ct-9406 132 10 jj jj PROPN ct-9406 132 11 , , PUNCT ct-9406 132 12 et et PROPN ct-9406 132 13 al al PROPN ct-9406 132 14 : : PUNCT ct-9406 132 15 seminal seminal ADJ ct-9406 132 16 plasma plasma NOUN ct-9406 132 17 : : PUNCT ct-9406 132 18 relevant relevant ADJ ct-9406 132 19 for for ADP ct-9406 132 20 fertility fertility NOUN ct-9406 132 21 ? ? PUNCT ct-9406 133 1 int int PROPN ct-9406 133 2 j j PROPN ct-9406 133 3 mol mol ADP ct-9406 133 4 sci sci PROPN ct-9406 133 5 2021;22:4368 2021;22:4368 PROPN ct-9406 133 6 . . PUNCT ct-9406 133 7 doi doi NOUN ct-9406 133 8 : : PUNCT ct-9406 133 9 10.3390 10.3390 NUM ct-9406 133 10 / / SYM ct-9406 133 11 ijms22094368 ijms22094368 PROPN ct-9406 133 12 11 11 NUM ct-9406 133 13 . . PUNCT ct-9406 134 1 rodriguez rodriguez NOUN ct-9406 134 2 - - PUNCT ct-9406 134 3 martinez martinez PROPN ct-9406 134 4 h h PROPN ct-9406 134 5 , , PUNCT ct-9406 134 6 roca roca PROPN ct-9406 134 7 j j PROPN ct-9406 134 8 : : PUNCT ct-9406 134 9 extracellular extracellular ADJ ct-9406 134 10 vesicles vesicle NOUN ct-9406 134 11 in in ADP ct-9406 134 12 seminal seminal ADJ ct-9406 134 13 plasma plasma NOUN ct-9406 134 14 : : PUNCT ct-9406 134 15 a a DET ct-9406 134 16 safe safe ADJ ct-9406 134 17 and and CCONJ ct-9406 134 18 relevant relevant ADJ ct-9406 134 19 tool tool NOUN ct-9406 134 20 to to PART ct-9406 134 21 improve improve VERB ct-9406 134 22 fertility fertility NOUN ct-9406 134 23 in in ADP ct-9406 134 24 livestock livestock NOUN ct-9406 134 25 ? ? PUNCT ct-9406 135 1 anim anim NOUN ct-9406 135 2 reprod reprod PROPN ct-9406 135 3 sci sci PROPN ct-9406 135 4 2022;244:107051 2022;244:107051 NUM ct-9406 135 5 . . PUNCT ct-9406 136 1 doi doi NOUN ct-9406 136 2 : : PUNCT ct-9406 136 3 10.1016 10.1016 NUM ct-9406 136 4 / / SYM ct-9406 136 5 j.anireprosci j.anireprosci NOUN ct-9406 136 6 . . PUNCT ct-9406 137 1 2022.107051 2022.107051 NUM ct-9406 137 2 12 12 NUM ct-9406 137 3 . . PUNCT ct-9406 138 1 pena pena PROPN ct-9406 138 2 fj fj PROPN ct-9406 138 3 , , PUNCT ct-9406 138 4 saravia saravia PROPN ct-9406 138 5 f f PROPN ct-9406 138 6 , , PUNCT ct-9406 138 7 johannisson johannisson PROPN ct-9406 138 8 a a PROPN ct-9406 138 9 , , PUNCT ct-9406 138 10 et et PROPN ct-9406 138 11 al al PROPN ct-9406 138 12 : : PUNCT ct-9406 138 13 detection detection NOUN ct-9406 138 14 of of ADP ct-9406 138 15 early early ADJ ct-9406 138 16 changes change NOUN ct-9406 138 17 in in ADP ct-9406 138 18 sperm sperm NOUN ct-9406 138 19 membrane membrane NOUN ct-9406 138 20 integrity integrity NOUN ct-9406 138 21 pre pre VERB ct-9406 138 22 - - ADJ ct-9406 138 23 freezing freezing ADJ ct-9406 138 24 can can AUX ct-9406 138 25 estimate estimate VERB ct-9406 138 26 post post ADJ ct-9406 138 27 - - ADJ ct-9406 138 28 thaw thaw ADJ ct-9406 138 29 quality quality NOUN ct-9406 138 30 of of ADP ct-9406 138 31 boar boar NOUN ct-9406 138 32 spermatozoa spermatozoon NOUN ct-9406 138 33 . . PUNCT ct-9406 139 1 anim anim PROPN ct-9406 139 2 reprod reprod PROPN ct-9406 139 3 sci sci PROPN ct-9406 139 4 2007;97:74–83 2007;97:74–83 PROPN ct-9406 139 5 . . PUNCT ct-9406 140 1 doi doi PROPN ct-9406 140 2 : : PUNCT ct-9406 140 3 10.1016 10.1016 NUM ct-9406 140 4 / / SYM ct-9406 140 5 j.anireprosci.2005.12.014 j.anireprosci.2005.12.014 PROPN ct-9406 140 6 13 13 NUM ct-9406 140 7 . . PUNCT ct-9406 141 1 da da PROPN ct-9406 141 2 silva silva PROPN ct-9406 141 3 bcm bcm PROPN ct-9406 141 4 , , PUNCT ct-9406 141 5 ortega ortega PROPN ct-9406 141 6 - - PROPN ct-9406 141 7 ferrusola ferrusola PROPN ct-9406 141 8 c c PROPN ct-9406 141 9 , , PUNCT ct-9406 141 10 morrell morrell PROPN ct-9406 141 11 jm jm PROPN ct-9406 141 12 , , PUNCT ct-9406 141 13 et et PROPN ct-9406 141 14 al al PROPN ct-9406 141 15 : : PUNCT ct-9406 141 16 flow flow VERB ct-9406 141 17 cytometric cytometric ADJ ct-9406 141 18 chromosomal chromosomal ADJ ct-9406 141 19 sex sex NOUN ct-9406 141 20 sorting sorting NOUN ct-9406 141 21 of of ADP ct-9406 141 22 stallion stallion NOUN ct-9406 141 23 spermatozoa spermatozoa NOUN ct-9406 141 24 induces induce VERB ct-9406 141 25 oxidative oxidative ADJ ct-9406 141 26 stress stress NOUN ct-9406 141 27 on on ADP ct-9406 141 28 mitochondria mitochondria NOUN ct-9406 141 29 and and CCONJ ct-9406 141 30 genomic genomic ADJ ct-9406 141 31 dna dna PROPN ct-9406 141 32 . . PUNCT ct-9406 142 1 reprod reprod PROPN ct-9406 142 2 domest domest PROPN ct-9406 142 3 anim anim PROPN ct-9406 142 4 2016;51:18–25 2016;51:18–25 PROPN ct-9406 142 5 . . PUNCT ct-9406 143 1 doi doi NOUN ct-9406 143 2 : : PUNCT ct-9406 143 3 10.1111 10.1111 NUM ct-9406 143 4 / / SYM ct-9406 143 5 rda.12640 rda.12640 NOUN ct-9406 143 6 14 14 NUM ct-9406 143 7 . . PUNCT ct-9406 144 1 gardela gardela PROPN ct-9406 144 2 j j PROPN ct-9406 144 3 , , PUNCT ct-9406 144 4 ruiz ruiz NOUN ct-9406 144 5 - - PUNCT ct-9406 144 6 conca conca PROPN ct-9406 144 7 m m PROPN ct-9406 144 8 , , PUNCT ct-9406 144 9 wright wright PROPN ct-9406 144 10 d d PROPN ct-9406 144 11 , , PUNCT ct-9406 144 12 et et PROPN ct-9406 144 13 al al PROPN ct-9406 144 14 ; ; PUNCT ct-9406 144 15 semen semen NOUN ct-9406 144 16 modulates modulate NOUN ct-9406 144 17 cell cell NOUN ct-9406 144 18 proliferation proliferation NOUN ct-9406 144 19 and and CCONJ ct-9406 144 20 differentiation differentiation NOUN ct-9406 144 21 - - PUNCT ct-9406 144 22 related relate VERB ct-9406 144 23 transcripts transcript NOUN ct-9406 144 24 in in ADP ct-9406 144 25 the the DET ct-9406 144 26 pig pig NOUN ct-9406 144 27 peri peri NOUN ct-9406 144 28 - - PUNCT ct-9406 144 29 ovulatory ovulatory ADJ ct-9406 144 30 endometrium endometrium NOUN ct-9406 144 31 . . PUNCT ct-9406 145 1 biology biology NOUN ct-9406 145 2 ( ( PUNCT ct-9406 145 3 basel basel PROPN ct-9406 145 4 ) ) PUNCT ct-9406 145 5 2022;11:616 2022;11:616 NUM ct-9406 145 6 . . PUNCT ct-9406 145 7 doi doi NOUN ct-9406 145 8 : : PUNCT ct-9406 145 9 10.3390 10.3390 NUM ct-9406 145 10 / / SYM ct-9406 145 11 biology11040616 biology11040616 PROPN ct-9406 145 12 15 15 NUM ct-9406 145 13 . . PUNCT ct-9406 146 1 alvarez alvarez PROPN ct-9406 146 2 - - PUNCT ct-9406 146 3 rodriguez rodriguez PROPN ct-9406 146 4 m m PROPN ct-9406 146 5 , , PUNCT ct-9406 146 6 martinez martinez PROPN ct-9406 146 7 c c PROPN ct-9406 146 8 , , PUNCT ct-9406 146 9 wright wright PROPN ct-9406 146 10 d d PROPN ct-9406 146 11 , , PUNCT ct-9406 146 12 et et PROPN ct-9406 146 13 al al PROPN ct-9406 146 14 : : PUNCT ct-9406 146 15 the the DET ct-9406 146 16 transcriptome transcriptome NOUN ct-9406 146 17 of of ADP ct-9406 146 18 pig pig NOUN ct-9406 146 19 spermatozoa spermatozoon NOUN ct-9406 146 20 , , PUNCT ct-9406 146 21 and and CCONJ ct-9406 146 22 its its PRON ct-9406 146 23 role role NOUN ct-9406 146 24 in in ADP ct-9406 146 25 fertility fertility NOUN ct-9406 146 26 . . PUNCT ct-9406 147 1 int int PROPN ct-9406 147 2 j j PROPN ct-9406 147 3 mol mol ADP ct-9406 147 4 sci sci PROPN ct-9406 147 5 2020;21:1572 2020;21:1572 PROPN ct-9406 147 6 . . PUNCT ct-9406 148 1 doi doi NOUN ct-9406 148 2 : : PUNCT ct-9406 148 3 10.3390 10.3390 NUM ct-9406 148 4 / / SYM ct-9406 148 5 ijms21051572 ijms21051572 PROPN ct-9406 148 6 16 16 NUM ct-9406 148 7 . . PUNCT ct-9406 149 1 martinez martinez PROPN ct-9406 149 2 ca ca PROPN ct-9406 149 3 , , PUNCT ct-9406 149 4 roca roca PROPN ct-9406 149 5 j j PROPN ct-9406 149 6 , , PUNCT ct-9406 149 7 alvarez alvarez PROPN ct-9406 149 8 - - PUNCT ct-9406 149 9 rodriguez rodriguez PROPN ct-9406 149 10 m m PROPN ct-9406 149 11 , , PUNCT ct-9406 149 12 et et PROPN ct-9406 149 13 al al PROPN ct-9406 149 14 : : PUNCT ct-9406 149 15 mirna mirna NOUN ct-9406 149 16 - - PUNCT ct-9406 149 17 profiling profiling NOUN ct-9406 149 18 in in ADP ct-9406 149 19 ejaculated ejaculate VERB ct-9406 149 20 and and CCONJ ct-9406 149 21 epididymal epididymal ADJ ct-9406 149 22 pig pig NOUN ct-9406 149 23 spermatozoa spermatozoon NOUN ct-9406 149 24 and and CCONJ ct-9406 149 25 their their PRON ct-9406 149 26 relation relation NOUN ct-9406 149 27 to to ADP ct-9406 149 28 fertility fertility NOUN ct-9406 149 29 after after ADP ct-9406 149 30 artificial artificial ADJ ct-9406 149 31 insemination insemination NOUN ct-9406 149 32 . . PUNCT ct-9406 150 1 biology biology NOUN ct-9406 150 2 ( ( PUNCT ct-9406 150 3 basel basel PROPN ct-9406 150 4 ) ) PUNCT ct-9406 150 5 2022;11:236 2022;11:236 PROPN ct-9406 150 6 . . PUNCT ct-9406 150 7 doi doi PROPN ct-9406 150 8 : : PUNCT ct-9406 150 9 10.3390 10.3390 NUM ct-9406 150 10 / / SYM ct-9406 150 11 biology11020236 biology11020236 NUM ct-9406 150 12 17 17 NUM ct-9406 150 13 . . PUNCT ct-9406 151 1 pertille pertille PROPN ct-9406 151 2 f f X ct-9406 151 3 , , PUNCT ct-9406 151 4 alvarez alvarez PROPN ct-9406 151 5 - - PUNCT ct-9406 151 6 rodriguez rodriguez PROPN ct-9406 151 7 m m PROPN ct-9406 151 8 , , PUNCT ct-9406 151 9 da da PROPN ct-9406 151 10 silva silva PROPN ct-9406 151 11 an an PROPN ct-9406 151 12 , , PUNCT ct-9406 151 13 et et PROPN ct-9406 151 14 al al PROPN ct-9406 151 15 : : PUNCT ct-9406 151 16 sperm sperm NOUN ct-9406 151 17 methylome methylome ADJ ct-9406 151 18 profiling profiling NOUN ct-9406 151 19 can can AUX ct-9406 151 20 discern discern VERB ct-9406 151 21 fertility fertility NOUN ct-9406 151 22 levels level NOUN ct-9406 151 23 in in ADP ct-9406 151 24 the the DET ct-9406 151 25 porcine porcine NOUN ct-9406 151 26 biomedical biomedical ADJ ct-9406 151 27 model model NOUN ct-9406 151 28 . . PUNCT ct-9406 152 1 int int PROPN ct-9406 152 2 j j PROPN ct-9406 152 3 mol mol ADP ct-9406 152 4 sci sci PROPN ct-9406 152 5 2021;22:2679 2021;22:2679 PROPN ct-9406 152 6 . . PUNCT ct-9406 152 7 doi doi NOUN ct-9406 152 8 : : PUNCT ct-9406 152 9 10.3390 10.3390 NUM ct-9406 152 10 / / SYM ct-9406 152 11 ijms22052679 ijms22052679 PROPN ct-9406 152 12 18 18 NUM ct-9406 152 13 . . PUNCT ct-9406 153 1 kerns kerns PROPN ct-9406 153 2 k k PROPN ct-9406 153 3 , , PUNCT ct-9406 153 4 zigo zigo PROPN ct-9406 153 5 m m PROPN ct-9406 153 6 , , PUNCT ct-9406 153 7 drobnis drobnis PROPN ct-9406 153 8 ez ez PROPN ct-9406 153 9 , , PUNCT ct-9406 153 10 et et PROPN ct-9406 153 11 al al PROPN ct-9406 153 12 : : PUNCT ct-9406 153 13 zinc zinc NOUN ct-9406 153 14 ion ion NOUN ct-9406 153 15 flux flux NOUN ct-9406 153 16 during during ADP ct-9406 153 17 mammalian mammalian ADJ ct-9406 153 18 sperm sperm NOUN ct-9406 153 19 capacitation capacitation NOUN ct-9406 153 20 . . PUNCT ct-9406 154 1 nat nat PROPN ct-9406 154 2 commun commun PROPN ct-9406 154 3 2018;9:2061 2018;9:2061 PROPN ct-9406 154 4 . . PUNCT ct-9406 155 1 doi doi PROPN ct-9406 155 2 : : PUNCT ct-9406 155 3 10.1038/ 10.1038/ NUM ct-9406 155 4 s41467 s41467 NOUN ct-9406 155 5 - - PUNCT ct-9406 155 6 018 018 NUM ct-9406 155 7 - - PUNCT ct-9406 155 8 04523 04523 NUM ct-9406 155 9 - - PUNCT ct-9406 155 10 y y NOUN ct-9406 155 11 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 NOUN ct-9406 155 12 https://doi.org/10.1111/j.1439-0531.2008.01186.x https://doi.org/10.1111/j.1439-0531.2008.01186.x PUNCT ct-9406 155 13 https://doi.org/10.1111/j.1439-0531.2008.01186.x https://doi.org/10.1111/j.1439-0531.2008.01186.x PROPN ct-9406 155 14 https://doi.org/10.1071/rd06104 https://doi.org/10.1071/rd06104 PRON ct-9406 156 1 https://doi.org/10.5661/rdr-vi-39 https://doi.org/10.5661/rdr-vi-39 PROPN ct-9406 156 2 https://doi.org/10.1016/j.theriogenology.2008.04.049 https://doi.org/10.1016/j.theriogenology.2008.04.049 PROPN ct-9406 156 3 https://doi.org/10.1016/j.theriogenology.2008.04.049 https://doi.org/10.1016/j.theriogenology.2008.04.049 PROPN ct-9406 156 4 https://doi.org/10.1016/j.anireprosci.2021.106838 https://doi.org/10.1016/j.anireprosci.2021.106838 X ct-9406 156 5 https://doi.org/10.1016/j.anireprosci.2021.106838 https://doi.org/10.1016/j.anireprosci.2021.106838 X ct-9406 156 6 https://doi.org/10.1111/j.1600-0897.2011.01033.x https://doi.org/10.1111/j.1600-0897.2011.01033.x NOUN ct-9406 156 7 https://doi.org/10.1021/acs.jproteome.7b00728 https://doi.org/10.1021/acs.jproteome.7b00728 NOUN ct-9406 156 8 https://doi.org/10.3390/ijms22094368 https://doi.org/10.3390/ijms22094368 NOUN ct-9406 156 9 https://doi.org/10.1016/j.anireprosci.2022.107051 https://doi.org/10.1016/j.anireprosci.2022.107051 ADJ ct-9406 156 10 https://doi.org/10.1016/j.anireprosci.2022.107051 https://doi.org/10.1016/j.anireprosci.2022.107051 ADJ ct-9406 156 11 https://doi.org/10.1016/j.anireprosci.2005.12.014 https://doi.org/10.1016/j.anireprosci.2005.12.014 PROPN ct-9406 156 12 https://doi.org/10.1111/rda.12640 https://doi.org/10.1111/rda.12640 PRON ct-9406 156 13 https://doi.org/10.3390/biology11040616 https://doi.org/10.3390/biology11040616 NOUN ct-9406 156 14 https://doi.org/10.3390/ijms21051572 https://doi.org/10.3390/ijms21051572 NOUN ct-9406 156 15 https://doi.org/10.3390/biology11020236 https://doi.org/10.3390/biology11020236 NOUN ct-9406 156 16 https://doi.org/10.3390/ijms22052679 https://doi.org/10.3390/ijms22052679 NOUN ct-9406 156 17 https://doi.org/10.1038/s41467-018-04523-y https://doi.org/10.1038/s41467-018-04523-y PROPN ct-9406 156 18 https://doi.org/10.1038/s41467-018-04523-y https://doi.org/10.1038/s41467-018-04523-y PROPN ct-9406 156 19 citation citation NOUN ct-9406 156 20 line line NOUN ct-9406 156 21 : : PUNCT ct-9406 156 22 clinical clinical ADJ ct-9406 156 23 theriogenology theriogenology NOUN ct-9406 156 24 2023 2023 NUM ct-9406 156 25 , , PUNCT ct-9406 156 26 15 15 NUM ct-9406 156 27 , , PUNCT ct-9406 156 28 9406 9406 NUM ct-9406 156 29 , , PUNCT ct-9406 156 30 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 VERB ct-9406 156 31 7 7 NUM ct-9406 156 32 19 19 NUM ct-9406 156 33 . . PUNCT ct-9406 156 34 zigo zigo PROPN ct-9406 156 35 m m PROPN ct-9406 156 36 , , PUNCT ct-9406 156 37 kerns kerns PROPN ct-9406 156 38 k k PROPN ct-9406 156 39 , , PUNCT ct-9406 156 40 sen sen PROPN ct-9406 156 41 s s PROPN ct-9406 156 42 , , PUNCT ct-9406 156 43 et et PROPN ct-9406 156 44 al al PROPN ct-9406 156 45 : : PUNCT ct-9406 156 46 zinc zinc NOUN ct-9406 156 47 is be AUX ct-9406 156 48 a a DET ct-9406 156 49 master master NOUN ct-9406 156 50 - - PUNCT ct-9406 156 51 regulator regulator NOUN ct-9406 156 52 of of ADP ct-9406 156 53 sperm sperm NOUN ct-9406 156 54 function function NOUN ct-9406 156 55 associated associate VERB ct-9406 156 56 with with ADP ct-9406 156 57 binding bind VERB ct-9406 156 58 , , PUNCT ct-9406 156 59 motility motility NOUN ct-9406 156 60 , , PUNCT ct-9406 156 61 and and CCONJ ct-9406 156 62 metabolic metabolic NOUN ct-9406 156 63 modulation modulation NOUN ct-9406 156 64 during during ADP ct-9406 156 65 porcine porcine NOUN ct-9406 156 66 sperm sperm NOUN ct-9406 156 67 capacitation capacitation NOUN ct-9406 156 68 . . PUNCT ct-9406 157 1 commun commun PROPN ct-9406 157 2 biol biol PROPN ct-9406 157 3 2022;5:538 2022;5:538 PROPN ct-9406 157 4 . . PUNCT ct-9406 158 1 doi doi NOUN ct-9406 158 2 : : PUNCT ct-9406 158 3 10.1038 10.1038 NUM ct-9406 158 4 / / SYM ct-9406 158 5 s42003 s42003 PROPN ct-9406 158 6 - - PUNCT ct-9406 158 7 022 022 NUM ct-9406 158 8 - - PUNCT ct-9406 158 9 03485 03485 NUM ct-9406 158 10 - - SYM ct-9406 158 11 8 8 NUM ct-9406 158 12 20 20 NUM ct-9406 158 13 . . PUNCT ct-9406 159 1 hossain hossain PROPN ct-9406 159 2 ms ms PROPN ct-9406 159 3 , , PUNCT ct-9406 159 4 johannisson johannisson PROPN ct-9406 159 5 a a PRON ct-9406 159 6 , , PUNCT ct-9406 159 7 wallgren wallgren PROPN ct-9406 159 8 m m PROPN ct-9406 159 9 , , PUNCT ct-9406 159 10 et et PROPN ct-9406 159 11 al al PROPN ct-9406 159 12 : : PUNCT ct-9406 159 13 flow flow VERB ct-9406 159 14 cytometry cytometry NOUN ct-9406 159 15 for for ADP ct-9406 159 16 the the DET ct-9406 159 17 assessment assessment NOUN ct-9406 159 18 of of ADP ct-9406 159 19 animal animal NOUN ct-9406 159 20 sperm sperm NOUN ct-9406 159 21 integrity integrity NOUN ct-9406 159 22 and and CCONJ ct-9406 159 23 functionality functionality NOUN ct-9406 159 24 : : PUNCT ct-9406 159 25 state state NOUN ct-9406 159 26 of of ADP ct-9406 159 27 the the DET ct-9406 159 28 art art NOUN ct-9406 159 29 . . PUNCT ct-9406 160 1 asian asian PROPN ct-9406 160 2 j j PROPN ct-9406 160 3 androl androl PROPN ct-9406 160 4 2011;13:406–419 2011;13:406–419 PROPN ct-9406 160 5 . . PUNCT ct-9406 161 1 doi doi PROPN ct-9406 161 2 : : PUNCT ct-9406 161 3 10.1038/ 10.1038/ NUM ct-9406 161 4 aja.2011.15 aja.2011.15 NUM ct-9406 161 5 21 21 NUM ct-9406 161 6 . . PUNCT ct-9406 162 1 ortega ortega PROPN ct-9406 162 2 - - PROPN ct-9406 162 3 ferrusola ferrusola PROPN ct-9406 162 4 c c PROPN ct-9406 162 5 , , PUNCT ct-9406 162 6 gil gil PROPN ct-9406 162 7 mc mc PROPN ct-9406 162 8 , , PUNCT ct-9406 162 9 rodriguez rodriguez PROPN ct-9406 162 10 - - PUNCT ct-9406 162 11 martinez martinez PROPN ct-9406 162 12 h h PROPN ct-9406 162 13 , , PUNCT ct-9406 162 14 et et PROPN ct-9406 162 15 al al PROPN ct-9406 162 16 : : PUNCT ct-9406 162 17 flow flow NOUN ct-9406 162 18 cytometry cytometry NOUN ct-9406 162 19 in in ADP ct-9406 162 20 spermatology spermatology NOUN ct-9406 162 21 : : PUNCT ct-9406 162 22 a a DET ct-9406 162 23 bright bright ADJ ct-9406 162 24 future future NOUN ct-9406 162 25 ahead ahead ADV ct-9406 162 26 . . PUNCT ct-9406 163 1 reprod reprod PROPN ct-9406 163 2 domest domest PROPN ct-9406 163 3 anim anim PROPN ct-9406 163 4 2017;52:921–931 2017;52:921–931 PROPN ct-9406 163 5 . . PUNCT ct-9406 164 1 doi doi NOUN ct-9406 164 2 : : PUNCT ct-9406 164 3 10.1111 10.1111 NUM ct-9406 164 4 / / SYM ct-9406 164 5 rda.13043 rda.13043 NUM ct-9406 164 6 22 22 NUM ct-9406 164 7 . . PUNCT ct-9406 165 1 roca roca PROPN ct-9406 165 2 j j PROPN ct-9406 165 3 , , PUNCT ct-9406 165 4 parrilla parrilla PROPN ct-9406 165 5 i i PROPN ct-9406 165 6 , , PUNCT ct-9406 165 7 gil gil PROPN ct-9406 165 8 ma ma PROPN ct-9406 165 9 , , PUNCT ct-9406 165 10 et et PROPN ct-9406 165 11 al al PROPN ct-9406 165 12 : : PUNCT ct-9406 165 13 non non ADJ ct-9406 165 14 - - ADJ ct-9406 165 15 viable viable ADJ ct-9406 165 16 sperm sperm NOUN ct-9406 165 17 in in ADP ct-9406 165 18 the the DET ct-9406 165 19 ejaculate ejaculate NOUN ct-9406 165 20 : : PUNCT ct-9406 165 21 lethal lethal ADJ ct-9406 165 22 escorts escort NOUN ct-9406 165 23 for for ADP ct-9406 165 24 contemporary contemporary ADJ ct-9406 165 25 viable viable ADJ ct-9406 165 26 sperm sperm NOUN ct-9406 165 27 . . PUNCT ct-9406 166 1 anim anim NOUN ct-9406 166 2 reprod reprod PROPN ct-9406 166 3 sci sci PROPN ct-9406 166 4 2016;169:24–31 2016;169:24–31 PROPN ct-9406 166 5 . . PUNCT ct-9406 167 1 doi doi PROPN ct-9406 167 2 : : PUNCT ct-9406 167 3 10.1016 10.1016 NUM ct-9406 167 4 / / SYM ct-9406 167 5 j.anireprosci.2016.02.028 j.anireprosci.2016.02.028 PROPN ct-9406 167 6 23 23 NUM ct-9406 167 7 . . PUNCT ct-9406 168 1 pena pena PROPN ct-9406 168 2 fj fj PROPN ct-9406 168 3 , , PUNCT ct-9406 168 4 gibb gibb PROPN ct-9406 168 5 z z NOUN ct-9406 168 6 : : PUNCT ct-9406 168 7 oxidative oxidative ADJ ct-9406 168 8 stress stress NOUN ct-9406 168 9 and and CCONJ ct-9406 168 10 reproductive reproductive ADJ ct-9406 168 11 function function NOUN ct-9406 168 12 : : PUNCT ct-9406 168 13 oxidative oxidative ADJ ct-9406 168 14 stress stress NOUN ct-9406 168 15 and and CCONJ ct-9406 168 16 the the DET ct-9406 168 17 long long ADJ ct-9406 168 18 - - PUNCT ct-9406 168 19 term term NOUN ct-9406 168 20 storage storage NOUN ct-9406 168 21 of of ADP ct-9406 168 22 horse horse NOUN ct-9406 168 23 spermatozoa spermatozoon NOUN ct-9406 168 24 . . PUNCT ct-9406 169 1 reproduction reproduction NOUN ct-9406 169 2 2022;164 2022;164 NOUN ct-9406 169 3 : : PUNCT ct-9406 169 4 f135 f135 NUM ct-9406 169 5 – – PUNCT ct-9406 170 1 f144 f144 PROPN ct-9406 170 2 . . PUNCT ct-9406 170 3 doi doi PROPN ct-9406 170 4 : : PUNCT ct-9406 170 5 10.1530 10.1530 NUM ct-9406 170 6 / / SYM ct-9406 170 7 rep-22 rep-22 NOUN ct-9406 170 8 - - SYM ct-9406 170 9 0264 0264 NUM ct-9406 170 10 24 24 NUM ct-9406 170 11 . . PUNCT ct-9406 171 1 pena pena PROPN ct-9406 171 2 fj fj PROPN ct-9406 171 3 , , PUNCT ct-9406 171 4 o’flaherty o’flaherty PROPN ct-9406 171 5 c c PROPN ct-9406 171 6 , , PUNCT ct-9406 171 7 ortiz ortiz PROPN ct-9406 171 8 rodriguez rodriguez PROPN ct-9406 171 9 jm jm PROPN ct-9406 171 10 , , PUNCT ct-9406 171 11 et et PROPN ct-9406 171 12 al al PROPN ct-9406 171 13 : : PUNCT ct-9406 171 14 the the DET ct-9406 171 15 stallion stallion NOUN ct-9406 171 16 spermatozoa spermatozoa VERB ct-9406 171 17 : : PUNCT ct-9406 171 18 a a DET ct-9406 171 19 valuable valuable ADJ ct-9406 171 20 model model NOUN ct-9406 171 21 to to PART ct-9406 171 22 help help VERB ct-9406 171 23 understand understand VERB ct-9406 171 24 the the DET ct-9406 171 25 interplay interplay NOUN ct-9406 171 26 between between ADP ct-9406 171 27 metabolism metabolism NOUN ct-9406 171 28 and and CCONJ ct-9406 171 29 redox redox NOUN ct-9406 171 30 ( ( PUNCT ct-9406 171 31 de)regulation de)regulation NOUN ct-9406 171 32 in in ADP ct-9406 171 33 sperm sperm NOUN ct-9406 171 34 cells cell NOUN ct-9406 171 35 . . PUNCT ct-9406 172 1 antioxid antioxid VERB ct-9406 172 2 redox redox NOUN ct-9406 172 3 signal signal PROPN ct-9406 172 4 2022;37:521–537 2022;37:521–537 PROPN ct-9406 172 5 . . PUNCT ct-9406 173 1 doi doi NOUN ct-9406 173 2 : : PUNCT ct-9406 173 3 10.1089/ 10.1089/ NUM ct-9406 173 4 ars.2021.0092 ars.2021.0092 NUM ct-9406 173 5 25 25 NUM ct-9406 173 6 . . PUNCT ct-9406 174 1 gonzalez gonzalez PROPN ct-9406 174 2 - - PUNCT ct-9406 174 3 castro castro PROPN ct-9406 174 4 ra ra PROPN ct-9406 174 5 , , PUNCT ct-9406 174 6 pena pena PROPN ct-9406 174 7 fj fj PROPN ct-9406 174 8 , , PUNCT ct-9406 174 9 herickhoff herickhoff PROPN ct-9406 174 10 la la NOUN ct-9406 174 11 : : PUNCT ct-9406 174 12 validation validation NOUN ct-9406 174 13 of of ADP ct-9406 174 14 a a DET ct-9406 174 15 new new ADJ ct-9406 174 16 multiparametric multiparametric ADJ ct-9406 174 17 protocol protocol NOUN ct-9406 174 18 to to PART ct-9406 174 19 assess assess VERB ct-9406 174 20 viability viability NOUN ct-9406 174 21 , , PUNCT ct-9406 174 22 acrosome acrosome NOUN ct-9406 174 23 integrity integrity NOUN ct-9406 174 24 and and CCONJ ct-9406 174 25 mitochondrial mitochondrial ADJ ct-9406 174 26 activity activity NOUN ct-9406 174 27 in in ADP ct-9406 174 28 cooled cool VERB ct-9406 174 29 and and CCONJ ct-9406 174 30 frozen frozen ADJ ct-9406 174 31 thawed thaw VERB ct-9406 174 32 boar boar NOUN ct-9406 174 33 spermatozoa spermatozoon NOUN ct-9406 174 34 . . PUNCT ct-9406 175 1 cytometry cytometry PROPN ct-9406 175 2 b b PROPN ct-9406 175 3 clin clin PROPN ct-9406 175 4 cytom cytom PROPN ct-9406 175 5 2022;102:400–408 2022;102:400–408 NUM ct-9406 175 6 . . PUNCT ct-9406 176 1 doi doi NOUN ct-9406 176 2 : : PUNCT ct-9406 176 3 10.1002 10.1002 NUM ct-9406 176 4 / / SYM ct-9406 176 5 cyto.b.22058 cyto.b.22058 NOUN ct-9406 176 6 26 26 NUM ct-9406 176 7 . . PUNCT ct-9406 177 1 ortiz ortiz PROPN ct-9406 177 2 - - PUNCT ct-9406 177 3 rodriguez rodriguez PROPN ct-9406 177 4 jm jm PROPN ct-9406 177 5 , , PUNCT ct-9406 177 6 martin martin PROPN ct-9406 177 7 - - PUNCT ct-9406 177 8 cano cano PROPN ct-9406 177 9 fe fe PROPN ct-9406 177 10 , , PUNCT ct-9406 177 11 gaitskell gaitskell NOUN ct-9406 177 12 - - PUNCT ct-9406 177 13 phillips phillip NOUN ct-9406 177 14 g g NOUN ct-9406 177 15 , , PUNCT ct-9406 177 16 et et PROPN ct-9406 177 17 al al PROPN ct-9406 177 18 : : PUNCT ct-9406 177 19 the the DET ct-9406 177 20 slc7a11 slc7a11 NOUN ct-9406 177 21 : : PUNCT ct-9406 177 22 sperm sperm NOUN ct-9406 177 23 mitochondrial mitochondrial ADJ ct-9406 177 24 function function NOUN ct-9406 177 25 and and CCONJ ct-9406 177 26 non non ADJ ct-9406 177 27 - - ADJ ct-9406 177 28 canonical canonical ADJ ct-9406 177 29 glutamate glutamate ADJ ct-9406 177 30 metabolism metabolism NOUN ct-9406 177 31 . . PUNCT ct-9406 178 1 reproduction reproduction NOUN ct-9406 178 2 2020;160:803–818 2020;160:803–818 NUM ct-9406 178 3 . . PUNCT ct-9406 179 1 doi doi NOUN ct-9406 179 2 : : PUNCT ct-9406 179 3 10.1530 10.1530 NUM ct-9406 179 4 / / SYM ct-9406 179 5 rep-20 rep-20 ADV ct-9406 179 6 - - PUNCT ct-9406 179 7 0181 0181 NUM ct-9406 179 8 27 27 NUM ct-9406 179 9 . . PUNCT ct-9406 180 1 ortega ortega PROPN ct-9406 180 2 - - PROPN ct-9406 180 3 ferrusola ferrusola PROPN ct-9406 180 4 c c PROPN ct-9406 180 5 , , PUNCT ct-9406 180 6 martin martin PROPN ct-9406 180 7 munoz munoz PROPN ct-9406 180 8 p p PROPN ct-9406 180 9 , , PUNCT ct-9406 180 10 ortiz ortiz PROPN ct-9406 180 11 - - PUNCT ct-9406 180 12 rodriguez rodriguez PROPN ct-9406 180 13 jm jm PROPN ct-9406 180 14 , , PUNCT ct-9406 180 15 et et PROPN ct-9406 180 16 al al PROPN ct-9406 180 17 : : PUNCT ct-9406 180 18 depletion depletion NOUN ct-9406 180 19 of of ADP ct-9406 180 20 thiols thiol NOUN ct-9406 180 21 leads lead VERB ct-9406 180 22 to to ADP ct-9406 180 23 redox redox ADJ ct-9406 180 24 deregulation deregulation NOUN ct-9406 180 25 , , PUNCT ct-9406 180 26 production production NOUN ct-9406 180 27 of of ADP ct-9406 180 28 4 4 NUM ct-9406 180 29 - - PUNCT ct-9406 180 30 hydroxinonenal hydroxinonenal ADJ ct-9406 180 31 and and CCONJ ct-9406 180 32 sperm sperm NOUN ct-9406 180 33 senescence senescence NOUN ct-9406 180 34 : : PUNCT ct-9406 180 35 a a DET ct-9406 180 36 possible possible ADJ ct-9406 180 37 role role NOUN ct-9406 180 38 for for ADP ct-9406 180 39 gsh gsh PROPN ct-9406 180 40 regulation regulation NOUN ct-9406 180 41 in in ADP ct-9406 180 42 spermatozoadagger spermatozoadagger NOUN ct-9406 180 43 . . PUNCT ct-9406 181 1 biol biol PROPN ct-9406 181 2 reprod reprod PROPN ct-9406 181 3 2019;100 2019;100 NUM ct-9406 181 4 : : PUNCT ct-9406 181 5 1090–1107 1090–1107 NUM ct-9406 181 6 . . PUNCT ct-9406 182 1 doi doi NOUN ct-9406 182 2 : : PUNCT ct-9406 182 3 10.1093 10.1093 NUM ct-9406 182 4 / / SYM ct-9406 182 5 biolre biolre NOUN ct-9406 182 6 / / SYM ct-9406 182 7 ioy241 ioy241 PROPN ct-9406 182 8 28 28 NUM ct-9406 182 9 . . PUNCT ct-9406 183 1 aitken aitken PROPN ct-9406 183 2 rj rj PROPN ct-9406 183 3 , , PUNCT ct-9406 183 4 findlay findlay PROPN ct-9406 183 5 jk jk PROPN ct-9406 183 6 , , PUNCT ct-9406 183 7 hutt hutt PROPN ct-9406 183 8 kj kj PROPN ct-9406 183 9 , , PUNCT ct-9406 183 10 et et PROPN ct-9406 183 11 al al PROPN ct-9406 183 12 : : PUNCT ct-9406 183 13 apoptosis apoptosis NOUN ct-9406 183 14 in in ADP ct-9406 183 15 the the DET ct-9406 183 16 germ germ NOUN ct-9406 183 17 line line NOUN ct-9406 183 18 . . PUNCT ct-9406 184 1 reproduction reproduction NOUN ct-9406 184 2 2011;141:139–150 2011;141:139–150 NOUN ct-9406 184 3 . . PUNCT ct-9406 185 1 doi doi NOUN ct-9406 185 2 : : PUNCT ct-9406 185 3 10.1530/ 10.1530/ NUM ct-9406 185 4 rep-10 rep-10 PROPN ct-9406 185 5 - - PROPN ct-9406 185 6 0232 0232 NUM ct-9406 185 7 29 29 NUM ct-9406 185 8 . . PUNCT ct-9406 186 1 boerke boerke PROPN ct-9406 186 2 a a PRON ct-9406 186 3 , , PUNCT ct-9406 186 4 brouwers brouwer NOUN ct-9406 186 5 jf jf PROPN ct-9406 186 6 , , PUNCT ct-9406 186 7 olkkonen olkkonen PROPN ct-9406 186 8 vm vm PROPN ct-9406 186 9 , , PUNCT ct-9406 186 10 et et PROPN ct-9406 186 11 al al PROPN ct-9406 186 12 : : PUNCT ct-9406 186 13 involvement involvement NOUN ct-9406 186 14 of of ADP ct-9406 186 15 bicarbonate bicarbonate NOUN ct-9406 186 16 - - PUNCT ct-9406 186 17 induced induce VERB ct-9406 186 18 radical radical ADJ ct-9406 186 19 signaling signaling NOUN ct-9406 186 20 in in ADP ct-9406 186 21 oxysterol oxysterol NOUN ct-9406 186 22 formation formation NOUN ct-9406 186 23 and and CCONJ ct-9406 186 24 sterol sterol NOUN ct-9406 186 25 depletion depletion NOUN ct-9406 186 26 of of ADP ct-9406 186 27 capacitating capacitate VERB ct-9406 186 28 mammalian mammalian ADJ ct-9406 186 29 sperm sperm NOUN ct-9406 186 30 during during ADP ct-9406 186 31 in in ADP ct-9406 186 32 vitro vitro X ct-9406 186 33 fertilization fertilization NOUN ct-9406 186 34 . . PUNCT ct-9406 187 1 biol biol PROPN ct-9406 187 2 reprod reprod PROPN ct-9406 187 3 2013;88:21 2013;88:21 NUM ct-9406 187 4 . . PUNCT ct-9406 188 1 doi doi NOUN ct-9406 188 2 : : PUNCT ct-9406 188 3 10.1095 10.1095 NUM ct-9406 188 4 / / SYM ct-9406 188 5 biolreprod biolreprod NOUN ct-9406 188 6 . . PUNCT ct-9406 189 1 112.101253 112.101253 NUM ct-9406 189 2 30 30 NUM ct-9406 189 3 . . PUNCT ct-9406 190 1 aitken aitken PROPN ct-9406 190 2 rj rj PROPN ct-9406 190 3 : : PUNCT ct-9406 190 4 the the DET ct-9406 190 5 capacitation capacitation NOUN ct-9406 190 6 - - PUNCT ct-9406 190 7 apoptosis apoptosis NOUN ct-9406 190 8 highway highway NOUN ct-9406 190 9 : : PUNCT ct-9406 190 10 oxysterols oxysterol NOUN ct-9406 190 11 and and CCONJ ct-9406 190 12 mammalian mammalian ADJ ct-9406 190 13 sperm sperm NOUN ct-9406 190 14 function function NOUN ct-9406 190 15 . . PUNCT ct-9406 191 1 biol biol PROPN ct-9406 191 2 reprod reprod PROPN ct-9406 191 3 2011;85:9–12 2011;85:9–12 NUM ct-9406 191 4 . . PUNCT ct-9406 192 1 doi doi NOUN ct-9406 192 2 : : PUNCT ct-9406 192 3 10.1095 10.1095 NUM ct-9406 192 4 / / SYM ct-9406 192 5 biolreprod.111.092528 biolreprod.111.092528 PROPN ct-9406 192 6 31 31 NUM ct-9406 192 7 . . PUNCT ct-9406 193 1 zerbinati zerbinati PROPN ct-9406 193 2 c c PROPN ct-9406 193 3 , , PUNCT ct-9406 193 4 caponecchia caponecchia PROPN ct-9406 193 5 l l NOUN ct-9406 193 6 , , PUNCT ct-9406 193 7 puca puca PROPN ct-9406 193 8 r r PROPN ct-9406 193 9 , , PUNCT ct-9406 193 10 et et PROPN ct-9406 193 11 al al PROPN ct-9406 193 12 : : PUNCT ct-9406 193 13 mass mass ADJ ct-9406 193 14 spectrometry spectrometry NOUN ct-9406 193 15 profiling profiling NOUN ct-9406 193 16 of of ADP ct-9406 193 17 oxysterols oxysterol NOUN ct-9406 193 18 in in ADP ct-9406 193 19 human human ADJ ct-9406 193 20 sperm sperm NOUN ct-9406 193 21 identifies identify VERB ct-9406 193 22 25 25 NUM ct-9406 193 23 - - PUNCT ct-9406 193 24 hydroxycholesterol hydroxycholesterol NOUN ct-9406 193 25 as as ADP ct-9406 193 26 a a DET ct-9406 193 27 marker marker NOUN ct-9406 193 28 of of ADP ct-9406 193 29 sperm sperm NOUN ct-9406 193 30 function function NOUN ct-9406 193 31 . . PUNCT ct-9406 194 1 redox redox ADJ ct-9406 194 2 biol biol PROPN ct-9406 194 3 2017;11:111–117 2017;11:111–117 PROPN ct-9406 194 4 . . PUNCT ct-9406 195 1 doi doi NOUN ct-9406 195 2 : : PUNCT ct-9406 195 3 10.1016 10.1016 NUM ct-9406 195 4 / / SYM ct-9406 195 5 j.redox.2016.11.008 j.redox.2016.11.008 ADP ct-9406 195 6 32 32 NUM ct-9406 195 7 . . PUNCT ct-9406 196 1 luque luque PROPN ct-9406 196 2 gm gm PROPN ct-9406 196 3 , , PUNCT ct-9406 196 4 dalotto dalotto PROPN ct-9406 196 5 - - PUNCT ct-9406 196 6 moreno moreno PROPN ct-9406 196 7 t t PROPN ct-9406 196 8 , , PUNCT ct-9406 196 9 martin martin PROPN ct-9406 196 10 - - PUNCT ct-9406 196 11 hidalgo hidalgo PROPN ct-9406 196 12 d d PROPN ct-9406 196 13 , , PUNCT ct-9406 196 14 et et PROPN ct-9406 196 15 al al PROPN ct-9406 196 16 . . PUNCT ct-9406 197 1 only only ADV ct-9406 197 2 a a DET ct-9406 197 3 subpopulation subpopulation NOUN ct-9406 197 4 of of ADP ct-9406 197 5 mouse mouse NOUN ct-9406 197 6 sperm sperm NOUN ct-9406 197 7 displays display VERB ct-9406 197 8 a a DET ct-9406 197 9 rapid rapid ADJ ct-9406 197 10 increase increase NOUN ct-9406 197 11 in in ADP ct-9406 197 12 intracellular intracellular ADJ ct-9406 197 13 calcium calcium NOUN ct-9406 197 14 during during ADP ct-9406 197 15 capacitation capacitation NOUN ct-9406 197 16 . . PUNCT ct-9406 198 1 j j PROPN ct-9406 198 2 cell cell PROPN ct-9406 198 3 physiol physiol PROPN ct-9406 198 4 2018;233 2018;233 NUM ct-9406 198 5 : : PUNCT ct-9406 198 6 9685–9700 9685–9700 NUM ct-9406 198 7 . . PUNCT ct-9406 199 1 doi doi NOUN ct-9406 199 2 : : PUNCT ct-9406 199 3 10.1002 10.1002 NUM ct-9406 199 4 / / SYM ct-9406 199 5 jcp.26883 jcp.26883 PROPN ct-9406 199 6 33 33 NUM ct-9406 199 7 . . PUNCT ct-9406 200 1 escoffier escoffier PROPN ct-9406 200 2 j j PROPN ct-9406 200 3 , , PUNCT ct-9406 200 4 navarrete navarrete PROPN ct-9406 200 5 f f PROPN ct-9406 200 6 , , PUNCT ct-9406 200 7 haddad haddad PROPN ct-9406 200 8 d d PROPN ct-9406 200 9 , , PUNCT ct-9406 200 10 et et PROPN ct-9406 200 11 al al PROPN ct-9406 200 12 : : PUNCT ct-9406 200 13 flow flow NOUN ct-9406 200 14 cytometry cytometry NOUN ct-9406 200 15 analysis analysis NOUN ct-9406 200 16 reveals reveal VERB ct-9406 200 17 that that SCONJ ct-9406 200 18 only only ADV ct-9406 200 19 a a DET ct-9406 200 20 subpopulation subpopulation NOUN ct-9406 200 21 of of ADP ct-9406 200 22 mouse mouse NOUN ct-9406 200 23 sperm sperm NOUN ct-9406 200 24 undergoes undergo VERB ct-9406 200 25 hyperpolarization hyperpolarization NOUN ct-9406 200 26 during during ADP ct-9406 200 27 capacitation capacitation NOUN ct-9406 200 28 . . PUNCT ct-9406 201 1 biol biol PROPN ct-9406 201 2 reprod reprod PROPN ct-9406 201 3 2015;92:121 2015;92:121 PROPN ct-9406 201 4 . . PUNCT ct-9406 202 1 doi doi NOUN ct-9406 202 2 : : PUNCT ct-9406 202 3 10.1095 10.1095 NUM ct-9406 202 4 / / SYM ct-9406 202 5 biolreprod.114.127266 biolreprod.114.127266 VERB ct-9406 202 6 34 34 NUM ct-9406 202 7 . . PUNCT ct-9406 202 8 sui sui PROPN ct-9406 203 1 x x SYM ct-9406 203 2 , , PUNCT ct-9406 203 3 zhang zhang PROPN ct-9406 203 4 r r PROPN ct-9406 203 5 , , PUNCT ct-9406 203 6 liu liu PROPN ct-9406 203 7 s s PROPN ct-9406 203 8 , , PUNCT ct-9406 203 9 et et PROPN ct-9406 203 10 al al PROPN ct-9406 203 11 : : PUNCT ct-9406 203 12 rsl3 rsl3 PROPN ct-9406 203 13 drives drive NOUN ct-9406 203 14 ferroptosis ferroptosis VERB ct-9406 203 15 through through ADP ct-9406 203 16 gpx4 gpx4 NOUN ct-9406 203 17 inactivation inactivation NOUN ct-9406 203 18 and and CCONJ ct-9406 203 19 ros ros PROPN ct-9406 203 20 production production NOUN ct-9406 203 21 in in ADP ct-9406 203 22 colorectal colorectal ADJ ct-9406 203 23 cancer cancer NOUN ct-9406 203 24 . . PUNCT ct-9406 204 1 front front ADJ ct-9406 204 2 pharmacol pharmacol PROPN ct-9406 204 3 2018;9:1371 2018;9:1371 NUM ct-9406 204 4 . . PUNCT ct-9406 205 1 doi doi NOUN ct-9406 205 2 : : PUNCT ct-9406 205 3 10.3389 10.3389 NUM ct-9406 205 4 / / SYM ct-9406 205 5 fphar.2018.01371 fphar.2018.01371 NOUN ct-9406 205 6 35 35 NUM ct-9406 205 7 . . PUNCT ct-9406 206 1 ortiz ortiz PROPN ct-9406 206 2 - - PUNCT ct-9406 206 3 rodriguez rodriguez PROPN ct-9406 206 4 jm jm PROPN ct-9406 206 5 , , PUNCT ct-9406 206 6 martin martin PROPN ct-9406 206 7 - - PUNCT ct-9406 206 8 cano cano PROPN ct-9406 206 9 fe fe PROPN ct-9406 206 10 , , PUNCT ct-9406 206 11 ortega ortega PROPN ct-9406 206 12 - - PROPN ct-9406 206 13 ferrusola ferrusola PROPN ct-9406 206 14 c c PROPN ct-9406 206 15 , , PUNCT ct-9406 206 16 et et PROPN ct-9406 206 17 al al PROPN ct-9406 206 18 : : PUNCT ct-9406 206 19 the the DET ct-9406 206 20 incorporation incorporation NOUN ct-9406 206 21 of of ADP ct-9406 206 22 cystine cystine NOUN ct-9406 206 23 by by ADP ct-9406 206 24 the the DET ct-9406 206 25 soluble soluble ADJ ct-9406 206 26 carrier carrier NOUN ct-9406 206 27 family family NOUN ct-9406 206 28 7 7 NUM ct-9406 206 29 member member NOUN ct-9406 206 30 11 11 NUM ct-9406 206 31 ( ( PUNCT ct-9406 206 32 slc7a11 slc7a11 NOUN ct-9406 206 33 ) ) PUNCT ct-9406 206 34 is be AUX ct-9406 206 35 a a DET ct-9406 206 36 component component NOUN ct-9406 206 37 of of ADP ct-9406 206 38 the the DET ct-9406 206 39 redox redox ADJ ct-9406 206 40 regulatory regulatory ADJ ct-9406 206 41 mechanism mechanism NOUN ct-9406 206 42 in in ADP ct-9406 206 43 stallion stallion NOUN ct-9406 206 44 spermatozoadagger spermatozoadagger NOUN ct-9406 206 45 . . PUNCT ct-9406 207 1 biol biol PROPN ct-9406 207 2 reprod reprod PROPN ct-9406 207 3 2019;101:208–222 2019;101:208–222 NUM ct-9406 207 4 . . PUNCT ct-9406 208 1 doi doi NOUN ct-9406 208 2 : : PUNCT ct-9406 208 3 10.1093 10.1093 NUM ct-9406 208 4 / / SYM ct-9406 208 5 biolre biolre PROPN ct-9406 208 6 / / SYM ct-9406 208 7 ioz069 ioz069 PROPN ct-9406 208 8 36 36 NUM ct-9406 208 9 . . PUNCT ct-9406 209 1 ortiz ortiz PROPN ct-9406 209 2 - - PUNCT ct-9406 209 3 rodriguez rodriguez PROPN ct-9406 209 4 jm jm PROPN ct-9406 209 5 , , PUNCT ct-9406 209 6 da da PROPN ct-9406 209 7 silva silva PROPN ct-9406 209 8 bcm bcm PROPN ct-9406 209 9 , , PUNCT ct-9406 209 10 masot masot ADJ ct-9406 209 11 j j PROPN ct-9406 209 12 , , PUNCT ct-9406 209 13 et et PROPN ct-9406 209 14 al al PROPN ct-9406 209 15 : : PUNCT ct-9406 209 16 rosiglitazone rosiglitazone NOUN ct-9406 209 17 in in ADP ct-9406 209 18 the the DET ct-9406 209 19 thawing thaw VERB ct-9406 209 20 medium medium NOUN ct-9406 209 21 improves improve VERB ct-9406 209 22 mitochondrial mitochondrial ADJ ct-9406 209 23 function function NOUN ct-9406 209 24 in in ADP ct-9406 209 25 stallion stallion NOUN ct-9406 209 26 spermatozoa spermatozoa VERB ct-9406 209 27 through through ADP ct-9406 209 28 regulating regulate VERB ct-9406 209 29 akt akt PROPN ct-9406 209 30 phosphorylation phosphorylation NOUN ct-9406 209 31 and and CCONJ ct-9406 209 32 reduction reduction NOUN ct-9406 209 33 of of ADP ct-9406 209 34 caspase caspase PROPN ct-9406 209 35 3 3 X ct-9406 209 36 . . PUNCT ct-9406 210 1 plos plos PROPN ct-9406 210 2 one one NUM ct-9406 210 3 2019;14 2019;14 NUM ct-9406 210 4 : : PUNCT ct-9406 210 5 e0211994 e0211994 ADJ ct-9406 210 6 . . PUNCT ct-9406 210 7 doi doi NOUN ct-9406 210 8 : : PUNCT ct-9406 210 9 10.1371 10.1371 NUM ct-9406 210 10 / / SYM ct-9406 210 11 journal.pone.0211994 journal.pone.0211994 PROPN ct-9406 210 12 37 37 NUM ct-9406 210 13 . . PUNCT ct-9406 211 1 aitken aitken PROPN ct-9406 211 2 rj rj PROPN ct-9406 211 3 , , PUNCT ct-9406 211 4 baker baker PROPN ct-9406 211 5 ma ma PROPN ct-9406 211 6 , , PUNCT ct-9406 211 7 nixon nixon PROPN ct-9406 211 8 b b PROPN ct-9406 211 9 : : PUNCT ct-9406 211 10 are be AUX ct-9406 211 11 sperm sperm NOUN ct-9406 211 12 capacitation capacitation NOUN ct-9406 211 13 and and CCONJ ct-9406 211 14 apoptosis apoptosis VERB ct-9406 211 15 the the DET ct-9406 211 16 opposite opposite ADJ ct-9406 211 17 ends end NOUN ct-9406 211 18 of of ADP ct-9406 211 19 a a DET ct-9406 211 20 continuum continuum NOUN ct-9406 211 21 driven drive VERB ct-9406 211 22 by by ADP ct-9406 211 23 oxidative oxidative ADJ ct-9406 211 24 stress stress NOUN ct-9406 211 25 ? ? PUNCT ct-9406 212 1 asian asian PROPN ct-9406 212 2 j j PROPN ct-9406 212 3 androl androl PROPN ct-9406 212 4 2015;17:633–639 2015;17:633–639 PROPN ct-9406 212 5 . . PUNCT ct-9406 213 1 doi doi NOUN ct-9406 213 2 : : PUNCT ct-9406 213 3 10.4103/1008 10.4103/1008 NUM ct-9406 213 4 - - SYM ct-9406 213 5 682x.153850 682x.153850 NUM ct-9406 213 6 38 38 NUM ct-9406 213 7 . . PUNCT ct-9406 214 1 pena pena PROPN ct-9406 214 2 fj fj PROPN ct-9406 214 3 , , PUNCT ct-9406 214 4 johannisson johannisson PROPN ct-9406 214 5 a a PROPN ct-9406 214 6 , , PUNCT ct-9406 214 7 wallgren wallgren PROPN ct-9406 214 8 m m PROPN ct-9406 214 9 , , PUNCT ct-9406 214 10 et et PROPN ct-9406 214 11 al al PROPN ct-9406 214 12 : : PUNCT ct-9406 214 13 antioxidant antioxidant ADJ ct-9406 214 14 supplementation supplementation NOUN ct-9406 214 15 of of ADP ct-9406 214 16 boar boar NOUN ct-9406 214 17 spermatozoa spermatozoon NOUN ct-9406 214 18 from from ADP ct-9406 214 19 different different ADJ ct-9406 214 20 fractions fraction NOUN ct-9406 214 21 of of ADP ct-9406 214 22 the the DET ct-9406 214 23 ejaculate ejaculate NOUN ct-9406 214 24 improves improve VERB ct-9406 214 25 cryopreservation cryopreservation NOUN ct-9406 214 26 : : PUNCT ct-9406 214 27 changes change NOUN ct-9406 214 28 in in ADP ct-9406 214 29 sperm sperm NOUN ct-9406 214 30 membrane membrane NOUN ct-9406 214 31 lipid lipid NOUN ct-9406 214 32 architecture architecture NOUN ct-9406 214 33 . . PUNCT ct-9406 215 1 zygote zygote ADJ ct-9406 215 2 2004;12:117–124 2004;12:117–124 NOUN ct-9406 215 3 . . PUNCT ct-9406 215 4 doi doi NOUN ct-9406 215 5 : : PUNCT ct-9406 216 1 10.1017/ 10.1017/ NUM ct-9406 216 2 s096719940400262x s096719940400262x NOUN ct-9406 216 3 39 39 NUM ct-9406 216 4 . . PUNCT ct-9406 217 1 pena pena PROPN ct-9406 217 2 fj fj PROPN ct-9406 217 3 , , PUNCT ct-9406 217 4 johannisson johannisson PROPN ct-9406 217 5 a a PROPN ct-9406 217 6 , , PUNCT ct-9406 217 7 wallgren wallgren PROPN ct-9406 217 8 m m PROPN ct-9406 217 9 , , PUNCT ct-9406 217 10 et et PROPN ct-9406 217 11 al al PROPN ct-9406 217 12 : : PUNCT ct-9406 217 13 effect effect NOUN ct-9406 217 14 of of ADP ct-9406 217 15 hyaluronan hyaluronan PROPN ct-9406 217 16 supplementation supplementation NOUN ct-9406 217 17 on on ADP ct-9406 217 18 boar boar NOUN ct-9406 217 19 sperm sperm NOUN ct-9406 217 20 motility motility NOUN ct-9406 217 21 and and CCONJ ct-9406 217 22 membrane membrane NOUN ct-9406 217 23 lipid lipid NOUN ct-9406 217 24 architecture architecture NOUN ct-9406 217 25 status status NOUN ct-9406 217 26 after after ADP ct-9406 217 27 cryopreservation cryopreservation NOUN ct-9406 217 28 . . PUNCT ct-9406 218 1 theriogenology theriogenology NOUN ct-9406 218 2 2004;61:63–70 2004;61:63–70 NUM ct-9406 218 3 . . PUNCT ct-9406 219 1 doi doi NOUN ct-9406 219 2 : : PUNCT ct-9406 219 3 10.1016 10.1016 NUM ct-9406 219 4 / / SYM ct-9406 219 5 s0093 s0093 PROPN ct-9406 219 6 - - PUNCT ct-9406 219 7 691x(03)00181 691x(03)00181 PROPN ct-9406 219 8 - - PUNCT ct-9406 219 9 x x SYM ct-9406 219 10 40 40 NUM ct-9406 219 11 . . PUNCT ct-9406 220 1 tienthai tienthai PROPN ct-9406 220 2 p p X ct-9406 220 3 , , PUNCT ct-9406 220 4 johannisson johannisson PROPN ct-9406 220 5 a a PRON ct-9406 220 6 , , PUNCT ct-9406 220 7 rodriguez rodriguez PROPN ct-9406 220 8 - - PUNCT ct-9406 220 9 martinez martinez PROPN ct-9406 220 10 h h NOUN ct-9406 220 11 : : PUNCT ct-9406 220 12 sperm sperm NOUN ct-9406 220 13 capacitation capacitation NOUN ct-9406 220 14 in in ADP ct-9406 220 15 the the DET ct-9406 220 16 porcine porcine NOUN ct-9406 220 17 oviduct oviduct NOUN ct-9406 220 18 . . PUNCT ct-9406 221 1 anim anim NOUN ct-9406 221 2 reprod reprod PROPN ct-9406 221 3 sci sci PROPN ct-9406 221 4 2004;80:131–146 2004;80:131–146 PROPN ct-9406 221 5 . . PUNCT ct-9406 222 1 doi doi NOUN ct-9406 222 2 : : PUNCT ct-9406 222 3 10.1016 10.1016 NUM ct-9406 222 4 / / SYM ct-9406 222 5 s0378 s0378 PROPN ct-9406 222 6 - - PUNCT ct-9406 222 7 4320(03)00134 4320(03)00134 NUM ct-9406 222 8 - - SYM ct-9406 222 9 9 9 NUM ct-9406 222 10 41 41 NUM ct-9406 222 11 . . PUNCT ct-9406 223 1 gibbons gibbon NOUN ct-9406 223 2 sj sj PROPN ct-9406 223 3 , , PUNCT ct-9406 223 4 washburn washburn PROPN ct-9406 223 5 kb kb PROPN ct-9406 223 6 , , PUNCT ct-9406 223 7 talamo talamo NOUN ct-9406 223 8 br br NOUN ct-9406 223 9 : : PUNCT ct-9406 223 10 p2x(7 p2x(7 ADJ ct-9406 223 11 ) ) PUNCT ct-9406 223 12 receptors receptor NOUN ct-9406 223 13 in in ADP ct-9406 223 14 rat rat NOUN ct-9406 223 15 parotid parotid ADJ ct-9406 223 16 acinar acinar NOUN ct-9406 223 17 cells cell NOUN ct-9406 223 18 : : PUNCT ct-9406 223 19 formation formation NOUN ct-9406 223 20 of of ADP ct-9406 223 21 large large ADJ ct-9406 223 22 pores pore NOUN ct-9406 223 23 . . PUNCT ct-9406 224 1 j j PROPN ct-9406 224 2 auton auton PROPN ct-9406 224 3 pharmacol pharmacol PROPN ct-9406 224 4 2001;21:181–190 2001;21:181–190 NUM ct-9406 224 5 . . PUNCT ct-9406 225 1 doi doi NOUN ct-9406 225 2 : : PUNCT ct-9406 225 3 10.1046 10.1046 NUM ct-9406 225 4 / / SYM ct-9406 225 5 j.1365 j.1365 NOUN ct-9406 225 6 - - PUNCT ct-9406 225 7 2680.2001.00224.x 2680.2001.00224.x NUM ct-9406 225 8 42 42 NUM ct-9406 225 9 . . PUNCT ct-9406 226 1 xiao xiao PROPN ct-9406 226 2 f f PROPN ct-9406 226 3 , , PUNCT ct-9406 226 4 waldrop waldrop PROPN ct-9406 226 5 sl sl INTJ ct-9406 226 6 , , PUNCT ct-9406 226 7 khimji khimji PROPN ct-9406 226 8 ak ak PROPN ct-9406 226 9 , , PUNCT ct-9406 226 10 et et PROPN ct-9406 226 11 al al PROPN ct-9406 226 12 : : PUNCT ct-9406 226 13 pannexin1 pannexin1 PROPN ct-9406 226 14 contributes contribute VERB ct-9406 226 15 to to ADP ct-9406 226 16 pathophysiological pathophysiological ADJ ct-9406 226 17 atp atp NOUN ct-9406 226 18 release release NOUN ct-9406 226 19 in in ADP ct-9406 226 20 lipoapoptosis lipoapoptosis NOUN ct-9406 226 21 induced induce VERB ct-9406 226 22 by by ADP ct-9406 226 23 saturated saturate VERB ct-9406 226 24 free free ADJ ct-9406 226 25 fatty fatty NOUN ct-9406 226 26 acids acid NOUN ct-9406 226 27 in in ADP ct-9406 226 28 liver liver NOUN ct-9406 226 29 cells cell NOUN ct-9406 226 30 . . PUNCT ct-9406 227 1 am be AUX ct-9406 227 2 j j PROPN ct-9406 227 3 physiol physiol PROPN ct-9406 227 4 cell cell PROPN ct-9406 227 5 physiol physiol PROPN ct-9406 227 6 2012;303 2012;303 NUM ct-9406 227 7 : : PUNCT ct-9406 227 8 c1034 c1034 PROPN ct-9406 227 9 – – PUNCT ct-9406 227 10 c1044 c1044 PROPN ct-9406 227 11 . . PROPN ct-9406 227 12 doi doi PROPN ct-9406 227 13 : : PUNCT ct-9406 227 14 10.1152 10.1152 NUM ct-9406 227 15 / / SYM ct-9406 227 16 ajpcell.00175.2012 ajpcell.00175.2012 NOUN ct-9406 227 17 43 43 NUM ct-9406 227 18 . . PUNCT ct-9406 228 1 gallardo gallardo PROPN ct-9406 228 2 bjm bjm PROPN ct-9406 228 3 , , PUNCT ct-9406 228 4 da da PROPN ct-9406 228 5 silva silva PROPN ct-9406 228 6 bcm bcm PROPN ct-9406 228 7 , , PUNCT ct-9406 228 8 martin martin PROPN ct-9406 228 9 mp mp PROPN ct-9406 228 10 , , PUNCT ct-9406 228 11 et et PROPN ct-9406 228 12 al al PROPN ct-9406 228 13 : : PUNCT ct-9406 228 14 phosphorylated phosphorylate VERB ct-9406 228 15 akt akt PROPN ct-9406 228 16 preserves preserve VERB ct-9406 228 17 stallion stallion NOUN ct-9406 228 18 sperm sperm NOUN ct-9406 228 19 viability viability NOUN ct-9406 228 20 and and CCONJ ct-9406 228 21 motility motility NOUN ct-9406 228 22 by by ADP ct-9406 228 23 inhibiting inhibit VERB ct-9406 228 24 caspases caspase NOUN ct-9406 228 25 3 3 NUM ct-9406 228 26 and and CCONJ ct-9406 228 27 7 7 NUM ct-9406 228 28 . . X ct-9406 228 29 reproduction reproduction NOUN ct-9406 228 30 2014;148:221–235 2014;148:221–235 NUM ct-9406 228 31 . . PUNCT ct-9406 229 1 doi doi NOUN ct-9406 229 2 : : PUNCT ct-9406 229 3 10.1530/ 10.1530/ NUM ct-9406 229 4 rep-13 rep-13 NOUN ct-9406 229 5 - - PUNCT ct-9406 229 6 0191 0191 NUM ct-9406 229 7 44 44 NUM ct-9406 229 8 . . PUNCT ct-9406 230 1 da da PROPN ct-9406 230 2 silva silva PROPN ct-9406 230 3 bcm bcm PROPN ct-9406 230 4 , , PUNCT ct-9406 230 5 ortega ortega PROPN ct-9406 230 6 fc fc PROPN ct-9406 230 7 , , PUNCT ct-9406 230 8 morillo morillo PROPN ct-9406 230 9 ra ra PROPN ct-9406 230 10 , , PUNCT ct-9406 230 11 et et PROPN ct-9406 230 12 al al PROPN ct-9406 230 13 : : PUNCT ct-9406 230 14 sex sex NOUN ct-9406 230 15 sorting sorting NOUN ct-9406 230 16 increases increase VERB ct-9406 230 17 the the DET ct-9406 230 18 permeability permeability NOUN ct-9406 230 19 of of ADP ct-9406 230 20 the the DET ct-9406 230 21 membrane membrane NOUN ct-9406 230 22 of of ADP ct-9406 230 23 stallion stallion NOUN ct-9406 230 24 spermatozoa spermatozoon NOUN ct-9406 230 25 . . PUNCT ct-9406 231 1 anim anim PROPN ct-9406 231 2 reprod reprod PROPN ct-9406 231 3 sci sci PROPN ct-9406 231 4 2013;138:241–251 2013;138:241–251 NUM ct-9406 231 5 . . PUNCT ct-9406 232 1 doi doi NOUN ct-9406 232 2 : : PUNCT ct-9406 232 3 10.1016 10.1016 NUM ct-9406 232 4 / / SYM ct-9406 232 5 j.anireprosci.2013.02.021 j.anireprosci.2013.02.021 NUM ct-9406 232 6 45 45 NUM ct-9406 232 7 . . PUNCT ct-9406 233 1 van van PROPN ct-9406 233 2 der der PROPN ct-9406 233 3 paal paal PROPN ct-9406 233 4 j j PROPN ct-9406 233 5 , , PUNCT ct-9406 233 6 neyts neyts PROPN ct-9406 233 7 ec ec PROPN ct-9406 233 8 , , PUNCT ct-9406 233 9 verlackt verlackt VERB ct-9406 233 10 ccw ccw NOUN ct-9406 233 11 , , PUNCT ct-9406 233 12 et et PROPN ct-9406 233 13 al al PROPN ct-9406 233 14 : : PUNCT ct-9406 233 15 effect effect NOUN ct-9406 233 16 of of ADP ct-9406 233 17 lipid lipid ADJ ct-9406 233 18 peroxidation peroxidation NOUN ct-9406 233 19 on on ADP ct-9406 233 20 membrane membrane NOUN ct-9406 233 21 permeability permeability NOUN ct-9406 233 22 of of ADP ct-9406 233 23 cancer cancer NOUN ct-9406 233 24 and and CCONJ ct-9406 233 25 normal normal ADJ ct-9406 233 26 cells cell NOUN ct-9406 233 27 subjected subject VERB ct-9406 233 28 to to ADP ct-9406 233 29 oxidative oxidative ADJ ct-9406 233 30 stress stress NOUN ct-9406 233 31 . . PUNCT ct-9406 234 1 chem chem NOUN ct-9406 234 2 sci sci PROPN ct-9406 234 3 2016;7:489–498 2016;7:489–498 PROPN ct-9406 234 4 . . PUNCT ct-9406 235 1 doi doi NOUN ct-9406 235 2 : : PUNCT ct-9406 235 3 10.1039 10.1039 NUM ct-9406 235 4 / / SYM ct-9406 235 5 c5sc02311d c5sc02311d PROPN ct-9406 235 6 46 46 NUM ct-9406 235 7 . . PUNCT ct-9406 236 1 ribeiro ribeiro PROPN ct-9406 236 2 sc sc PROPN ct-9406 236 3 , , PUNCT ct-9406 236 4 sartorius sartorius NOUN ct-9406 236 5 g g NOUN ct-9406 236 6 , , PUNCT ct-9406 236 7 pletscher pletscher NOUN ct-9406 236 8 f f NOUN ct-9406 236 9 , , PUNCT ct-9406 236 10 et et PROPN ct-9406 236 11 al al PROPN ct-9406 236 12 : : PUNCT ct-9406 236 13 isolation isolation NOUN ct-9406 236 14 of of ADP ct-9406 236 15 spermatozoa spermatozoon NOUN ct-9406 236 16 with with ADP ct-9406 236 17 low low ADJ ct-9406 236 18 levels level NOUN ct-9406 236 19 of of ADP ct-9406 236 20 fragmented fragmented ADJ ct-9406 236 21 dna dna NOUN ct-9406 236 22 with with ADP ct-9406 236 23 the the DET ct-9406 236 24 use use NOUN ct-9406 236 25 of of ADP ct-9406 236 26 flow flow NOUN ct-9406 236 27 cytometry cytometry NOUN ct-9406 236 28 and and CCONJ ct-9406 236 29 sorting sorting NOUN ct-9406 236 30 . . PUNCT ct-9406 237 1 fertil fertil AUX ct-9406 237 2 steril steril VERB ct-9406 237 3 2013;100:686–694 2013;100:686–694 NUM ct-9406 237 4 . . PUNCT ct-9406 238 1 doi doi NOUN ct-9406 238 2 : : PUNCT ct-9406 238 3 10.1016 10.1016 NUM ct-9406 238 4 / / SYM ct-9406 238 5 j.fertnstert.2013.05.030 j.fertnstert.2013.05.030 PROPN ct-9406 238 6 47 47 NUM ct-9406 238 7 . . PUNCT ct-9406 239 1 ortega ortega PROPN ct-9406 239 2 fc fc PROPN ct-9406 239 3 , , PUNCT ct-9406 239 4 anel anel NOUN ct-9406 239 5 - - PUNCT ct-9406 239 6 lopez lopez NOUN ct-9406 239 7 l l NOUN ct-9406 239 8 , , PUNCT ct-9406 239 9 ortiz ortiz PROPN ct-9406 239 10 - - PUNCT ct-9406 239 11 rodriguez rodriguez PROPN ct-9406 239 12 jm jm PROPN ct-9406 239 13 , , PUNCT ct-9406 239 14 et et PROPN ct-9406 239 15 al al PROPN ct-9406 239 16 : : PUNCT ct-9406 239 17 stallion stallion NOUN ct-9406 239 18 spermatozoa spermatozoa NOUN ct-9406 239 19 surviving survive VERB ct-9406 239 20 freezing freezing NOUN ct-9406 239 21 and and CCONJ ct-9406 239 22 thawing thaw VERB ct-9406 239 23 experience experience NOUN ct-9406 239 24 membrane membrane NOUN ct-9406 239 25 depolarization depolarization NOUN ct-9406 239 26 and and CCONJ ct-9406 239 27 increased increase VERB ct-9406 239 28 intracellular intracellular ADJ ct-9406 239 29 na na ADP ct-9406 239 30 . . PUNCT ct-9406 239 31 andrology andrology PROPN ct-9406 239 32 2017;5:1174–1182 2017;5:1174–1182 PROPN ct-9406 239 33 . . PUNCT ct-9406 240 1 doi doi NOUN ct-9406 240 2 : : PUNCT ct-9406 240 3 10.1111 10.1111 NUM ct-9406 240 4 / / SYM ct-9406 240 5 andr.12419 andr.12419 PRON ct-9406 240 6 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 VERB ct-9406 240 7 https://doi.org/10.1038/s42003-022-03485-8 https://doi.org/10.1038/s42003-022-03485-8 PROPN ct-9406 240 8 https://doi.org/10.1038/aja.2011.15 https://doi.org/10.1038/aja.2011.15 ADP ct-9406 240 9 https://doi.org/10.1038/aja.2011.15 https://doi.org/10.1038/aja.2011.15 PROPN ct-9406 240 10 https://doi.org/10.1111/rda.13043 https://doi.org/10.1111/rda.13043 PROPN ct-9406 240 11 https://doi.org/10.1016/j.anireprosci.2016.02.028 https://doi.org/10.1016/j.anireprosci.2016.02.028 NOUN ct-9406 240 12 https://doi.org/10.1530/rep-22-0264 https://doi.org/10.1530/rep-22-0264 NOUN ct-9406 240 13 https://doi.org/10.1089/ars.2021.0092 https://doi.org/10.1089/ars.2021.0092 PROPN ct-9406 240 14 https://doi.org/10.1089/ars.2021.0092 https://doi.org/10.1089/ars.2021.0092 PROPN ct-9406 240 15 https://doi.org/10.1002/cyto.b.22058 https://doi.org/10.1002/cyto.b.22058 X ct-9406 240 16 https://doi.org/10.1530/rep-20-0181 https://doi.org/10.1530/rep-20-0181 PUNCT ct-9406 240 17 https://doi.org/10.1093/biolre/ioy241 https://doi.org/10.1093/biolre/ioy241 VERB ct-9406 240 18 https://doi.org/10.1530/rep-10-0232 https://doi.org/10.1530/rep-10-0232 PROPN ct-9406 240 19 https://doi.org/10.1530/rep-10-0232 https://doi.org/10.1530/rep-10-0232 PROPN ct-9406 240 20 https://doi.org/10.1095/biolreprod.112.101253 https://doi.org/10.1095/biolreprod.112.101253 PROPN ct-9406 240 21 https://doi.org/10.1095/biolreprod.112.101253 https://doi.org/10.1095/biolreprod.112.101253 PROPN ct-9406 240 22 https://doi.org/10.1095/biolreprod.111.092528 https://doi.org/10.1095/biolreprod.111.092528 PROPN ct-9406 240 23 https://doi.org/10.1016/j.redox.2016.11.008 https://doi.org/10.1016/j.redox.2016.11.008 PROPN ct-9406 240 24 https://doi.org/10.1002/jcp.26883 https://doi.org/10.1002/jcp.26883 PROPN ct-9406 240 25 https://doi.org/10.1095/biolreprod.114.127266 https://doi.org/10.1095/biolreprod.114.127266 NOUN ct-9406 240 26 https://doi.org/10.3389/fphar.2018.01371 https://doi.org/10.3389/fphar.2018.01371 PROPN ct-9406 240 27 https://doi.org/10.1093/biolre/ioz069 https://doi.org/10.1093/biolre/ioz069 PROPN ct-9406 240 28 https://doi.org/10.1371/journal.pone.0211994 https://doi.org/10.1371/journal.pone.0211994 PRON ct-9406 240 29 https://doi.org/10.1371/journal.pone.0211994 https://doi.org/10.1371/journal.pone.0211994 PUNCT ct-9406 240 30 https://doi.org/10.4103/1008-682x.153850 https://doi.org/10.4103/1008-682x.153850 PROPN ct-9406 240 31 https://doi.org/10.1017/s096719940400262x https://doi.org/10.1017/s096719940400262x PROPN ct-9406 240 32 https://doi.org/10.1017/s096719940400262x https://doi.org/10.1017/s096719940400262x PROPN ct-9406 240 33 https://doi.org/10.1016/s0093-691x(03)00181-x https://doi.org/10.1016/s0093-691x(03)00181-x PROPN ct-9406 241 1 https://doi.org/10.1016/s0378-4320(03)00134-9 https://doi.org/10.1016/s0378-4320(03)00134-9 ADJ ct-9406 241 2 https://doi.org/10.1046/j.1365-2680.2001.00224.x https://doi.org/10.1046/j.1365-2680.2001.00224.x PROPN ct-9406 241 3 https://doi.org/10.1152/ajpcell.00175.2012 https://doi.org/10.1152/ajpcell.00175.2012 PROPN ct-9406 241 4 https://doi.org/10.1530/rep-13-0191 https://doi.org/10.1530/rep-13-0191 PROPN ct-9406 241 5 https://doi.org/10.1530/rep-13-0191 https://doi.org/10.1530/rep-13-0191 PROPN ct-9406 241 6 https://doi.org/10.1016/j.anireprosci.2013.02.021 https://doi.org/10.1016/j.anireprosci.2013.02.021 PROPN ct-9406 241 7 https://doi.org/10.1039/c5sc02311d https://doi.org/10.1039/c5sc02311d NUM ct-9406 241 8 https://doi.org/10.1016/j.fertnstert.2013.05.030 https://doi.org/10.1016/j.fertnstert.2013.05.030 PROPN ct-9406 241 9 https://doi.org/10.1111/andr.12419 https://doi.org/10.1111/andr.12419 X ct-9406 241 10 8 8 NUM ct-9406 241 11 citation citation NOUN ct-9406 241 12 line line NOUN ct-9406 241 13 : : PUNCT ct-9406 241 14 clinical clinical ADJ ct-9406 241 15 theriogenology theriogenology NOUN ct-9406 241 16 2023 2023 NUM ct-9406 241 17 , , PUNCT ct-9406 241 18 15 15 NUM ct-9406 241 19 , , PUNCT ct-9406 241 20 9406 9406 NUM ct-9406 241 21 , , PUNCT ct-9406 241 22 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 VERB ct-9406 241 23 48 48 NUM ct-9406 241 24 . . PUNCT ct-9406 242 1 dasheiff dasheiff PROPN ct-9406 242 2 rm rm PROPN ct-9406 242 3 : : PUNCT ct-9406 242 4 a a DET ct-9406 242 5 new new ADJ ct-9406 242 6 method method NOUN ct-9406 242 7 of of ADP ct-9406 242 8 monitoring monitor VERB ct-9406 242 9 membrane membrane NOUN ct-9406 242 10 potential potential NOUN ct-9406 242 11 in in ADP ct-9406 242 12 rat rat NOUN ct-9406 242 13 hippocampal hippocampal ADJ ct-9406 242 14 slices slice NOUN ct-9406 242 15 using use VERB ct-9406 242 16 cyanine cyanine NOUN ct-9406 242 17 voltage voltage NOUN ct-9406 242 18 - - PUNCT ct-9406 242 19 sensitive sensitive ADJ ct-9406 242 20 dyes dye NOUN ct-9406 242 21 . . PUNCT ct-9406 243 1 j j PROPN ct-9406 243 2 neurosci neurosci PROPN ct-9406 243 3 methods method NOUN ct-9406 243 4 1985;13:199–212 1985;13:199–212 NUM ct-9406 243 5 . . PUNCT ct-9406 244 1 doi doi NOUN ct-9406 244 2 : : PUNCT ct-9406 244 3 10.1016/0165 10.1016/0165 NUM ct-9406 244 4 - - SYM ct-9406 244 5 0270(85)90068 0270(85)90068 NUM ct-9406 244 6 - - SYM ct-9406 244 7 8 8 NUM ct-9406 244 8 49 49 NUM ct-9406 244 9 . . PUNCT ct-9406 245 1 ortega ortega PROPN ct-9406 245 2 - - PROPN ct-9406 245 3 ferrusola ferrusola PROPN ct-9406 245 4 c c PROPN ct-9406 245 5 , , PUNCT ct-9406 245 6 anel anel NOUN ct-9406 245 7 - - PUNCT ct-9406 245 8 lopez lopez NOUN ct-9406 245 9 l l PROPN ct-9406 245 10 , , PUNCT ct-9406 245 11 martin martin PROPN ct-9406 245 12 - - PUNCT ct-9406 245 13 munoz munoz PROPN ct-9406 245 14 p p PROPN ct-9406 245 15 , , PUNCT ct-9406 245 16 et et PROPN ct-9406 245 17 al al PROPN ct-9406 245 18 : : PUNCT ct-9406 245 19 computational computational ADJ ct-9406 245 20 flow flow NOUN ct-9406 245 21 cytometry cytometry NOUN ct-9406 245 22 reveals reveal VERB ct-9406 245 23 that that SCONJ ct-9406 245 24 cryopreservation cryopreservation NOUN ct-9406 245 25 induces induce VERB ct-9406 245 26 spermptosis spermptosis NOUN ct-9406 245 27 but but CCONJ ct-9406 245 28 subpopulations subpopulation NOUN ct-9406 245 29 of of ADP ct-9406 245 30 spermatozoa spermatozoa NOUN ct-9406 245 31 may may AUX ct-9406 245 32 experience experience VERB ct-9406 245 33 capacitation capacitation NOUN ct-9406 245 34 - - PUNCT ct-9406 245 35 like like ADJ ct-9406 245 36 changes change NOUN ct-9406 245 37 . . PUNCT ct-9406 246 1 reproduction reproduction NOUN ct-9406 246 2 2017;153 2017;153 NOUN ct-9406 246 3 : : PUNCT ct-9406 247 1 293–304 293–304 NUM ct-9406 247 2 . . PUNCT ct-9406 247 3 doi doi NOUN ct-9406 247 4 : : PUNCT ct-9406 247 5 10.1530 10.1530 NUM ct-9406 247 6 / / SYM ct-9406 247 7 rep-16 rep-16 NOUN ct-9406 247 8 - - PUNCT ct-9406 247 9 0539 0539 NUM ct-9406 247 10 50 50 NUM ct-9406 247 11 . . PUNCT ct-9406 248 1 aitken aitken PROPN ct-9406 248 2 rj rj PROPN ct-9406 248 3 , , PUNCT ct-9406 248 4 gibb gibb PROPN ct-9406 248 5 z z PROPN ct-9406 248 6 , , PUNCT ct-9406 248 7 baker baker PROPN ct-9406 248 8 ma ma PROPN ct-9406 248 9 , , PUNCT ct-9406 248 10 et et PROPN ct-9406 248 11 al al PROPN ct-9406 248 12 : : PUNCT ct-9406 248 13 causes cause NOUN ct-9406 248 14 and and CCONJ ct-9406 248 15 consequences consequence NOUN ct-9406 248 16 of of ADP ct-9406 248 17 oxidative oxidative ADJ ct-9406 248 18 stress stress NOUN ct-9406 248 19 in in ADP ct-9406 248 20 spermatozoa spermatozoa NOUN ct-9406 248 21 . . PUNCT ct-9406 249 1 reprod reprod PROPN ct-9406 249 2 fertil fertil AUX ct-9406 249 3 dev dev PROPN ct-9406 249 4 2016;28:1–10 2016;28:1–10 NUM ct-9406 249 5 . . PUNCT ct-9406 250 1 doi doi NOUN ct-9406 250 2 : : PUNCT ct-9406 250 3 10.1071 10.1071 NUM ct-9406 250 4 / / SYM ct-9406 250 5 rd15325 rd15325 PROPN ct-9406 250 6 51 51 NUM ct-9406 250 7 . . PUNCT ct-9406 251 1 koppers koppers PROPN ct-9406 251 2 aj aj PROPN ct-9406 251 3 , , PUNCT ct-9406 251 4 mitchell mitchell PROPN ct-9406 251 5 la la PROPN ct-9406 251 6 , , PUNCT ct-9406 251 7 wang wang PROPN ct-9406 251 8 p p PROPN ct-9406 251 9 , , PUNCT ct-9406 251 10 et et PROPN ct-9406 251 11 al al PROPN ct-9406 251 12 : : PUNCT ct-9406 251 13 phosphoinositide phosphoinositide NOUN ct-9406 251 14 3 3 NUM ct-9406 251 15 - - PUNCT ct-9406 251 16 kinase kinase NOUN ct-9406 251 17 signalling signal VERB ct-9406 251 18 pathway pathway NOUN ct-9406 251 19 involvement involvement NOUN ct-9406 251 20 in in ADP ct-9406 251 21 a a DET ct-9406 251 22 truncated truncate VERB ct-9406 251 23 apoptotic apoptotic ADJ ct-9406 251 24 cascade cascade NOUN ct-9406 251 25 associated associate VERB ct-9406 251 26 with with ADP ct-9406 251 27 motility motility NOUN ct-9406 251 28 loss loss NOUN ct-9406 251 29 and and CCONJ ct-9406 251 30 oxidative oxidative ADJ ct-9406 251 31 dna dna NOUN ct-9406 251 32 damage damage NOUN ct-9406 251 33 in in ADP ct-9406 251 34 human human ADJ ct-9406 251 35 spermatozoa spermatozoon NOUN ct-9406 251 36 . . PUNCT ct-9406 252 1 biochem biochem PROPN ct-9406 252 2 j j PROPN ct-9406 252 3 2011;436:687–698 2011;436:687–698 NUM ct-9406 252 4 . . PUNCT ct-9406 253 1 doi doi NOUN ct-9406 253 2 : : PUNCT ct-9406 253 3 10.1042 10.1042 NUM ct-9406 253 4 / / SYM ct-9406 253 5 bj20110114 bj20110114 NOUN ct-9406 253 6 52 52 NUM ct-9406 253 7 . . PUNCT ct-9406 254 1 munoz munoz PROPN ct-9406 254 2 pm pm PROPN ct-9406 254 3 , , PUNCT ct-9406 254 4 ferrusola ferrusola PROPN ct-9406 254 5 co co PROPN ct-9406 254 6 , , PUNCT ct-9406 254 7 lopez lopez PROPN ct-9406 254 8 la la PROPN ct-9406 254 9 , , PUNCT ct-9406 254 10 et et PROPN ct-9406 254 11 al al PROPN ct-9406 254 12 : : PUNCT ct-9406 254 13 caspase caspase NOUN ct-9406 254 14 3 3 NUM ct-9406 254 15 activity activity NOUN ct-9406 254 16 and and CCONJ ct-9406 254 17 lipoperoxidative lipoperoxidative ADJ ct-9406 254 18 status status NOUN ct-9406 254 19 in in ADP ct-9406 254 20 raw raw ADJ ct-9406 254 21 semen semen NOUN ct-9406 254 22 predict predict VERB ct-9406 254 23 the the DET ct-9406 254 24 outcome outcome NOUN ct-9406 254 25 of of ADP ct-9406 254 26 cryopreservation cryopreservation NOUN ct-9406 254 27 of of ADP ct-9406 254 28 stallion stallion NOUN ct-9406 254 29 spermatozoa spermatozoon NOUN ct-9406 254 30 . . PUNCT ct-9406 255 1 biol biol PROPN ct-9406 255 2 reprod reprod PROPN ct-9406 255 3 2016;95:53 2016;95:53 NUM ct-9406 255 4 . . PUNCT ct-9406 256 1 doi doi NOUN ct-9406 256 2 : : PUNCT ct-9406 256 3 10.1095 10.1095 NUM ct-9406 256 4 / / SYM ct-9406 256 5 biolreprod.116.139444 biolreprod.116.139444 NOUN ct-9406 256 6 53 53 NUM ct-9406 256 7 . . PUNCT ct-9406 257 1 estaras estaras PROPN ct-9406 257 2 m m PROPN ct-9406 257 3 , , PUNCT ct-9406 257 4 pena pena PROPN ct-9406 257 5 fj fj PROPN ct-9406 257 6 , , PUNCT ct-9406 257 7 tapia tapia PROPN ct-9406 257 8 ja ja PROPN ct-9406 257 9 , , PUNCT ct-9406 257 10 et et PROPN ct-9406 257 11 al al PROPN ct-9406 257 12 : : PUNCT ct-9406 257 13 melatonin melatonin NOUN ct-9406 257 14 modulates modulate VERB ct-9406 257 15 proliferation proliferation NOUN ct-9406 257 16 of of ADP ct-9406 257 17 pancreatic pancreatic ADJ ct-9406 257 18 stellate stellate ADJ ct-9406 257 19 cells cell NOUN ct-9406 257 20 through through ADP ct-9406 257 21 caspase-3 caspase-3 NUM ct-9406 257 22 activation activation NOUN ct-9406 257 23 and and CCONJ ct-9406 257 24 changes change NOUN ct-9406 257 25 in in ADP ct-9406 257 26 cyclin cyclin PROPN ct-9406 257 27 a a PROPN ct-9406 257 28 and and CCONJ ct-9406 257 29 d d ADP ct-9406 257 30 expression expression NOUN ct-9406 257 31 . . PUNCT ct-9406 258 1 j j PROPN ct-9406 258 2 physiol physiol PROPN ct-9406 258 3 biochem biochem PROPN ct-9406 258 4 2020;76:345–355 2020;76:345–355 PROPN ct-9406 258 5 . . PUNCT ct-9406 259 1 doi doi NOUN ct-9406 259 2 : : PUNCT ct-9406 259 3 10.1007 10.1007 NUM ct-9406 259 4 / / SYM ct-9406 259 5 s13105 s13105 NOUN ct-9406 259 6 - - PUNCT ct-9406 259 7 020 020 NUM ct-9406 259 8 - - PUNCT ct-9406 259 9 00740 00740 NUM ct-9406 259 10 - - PUNCT ct-9406 259 11 6 6 NUM ct-9406 259 12 54 54 NUM ct-9406 259 13 . . PUNCT ct-9406 260 1 martin martin PROPN ct-9406 260 2 munoz munoz PROPN ct-9406 260 3 p p PROPN ct-9406 260 4 , , PUNCT ct-9406 260 5 ortega ortega PROPN ct-9406 260 6 ferrusola ferrusola PROPN ct-9406 260 7 c c PROPN ct-9406 260 8 , , PUNCT ct-9406 260 9 vizuete vizuete NOUN ct-9406 260 10 g g NOUN ct-9406 260 11 , , PUNCT ct-9406 260 12 et et PROPN ct-9406 260 13 al al PROPN ct-9406 260 14 : : PUNCT ct-9406 260 15 depletion depletion NOUN ct-9406 260 16 of of ADP ct-9406 260 17 intracellular intracellular ADJ ct-9406 260 18 thiols thiol NOUN ct-9406 260 19 and and CCONJ ct-9406 260 20 increased increase VERB ct-9406 260 21 production production NOUN ct-9406 260 22 of of ADP ct-9406 260 23 4 4 NUM ct-9406 260 24 - - PUNCT ct-9406 260 25 hydroxynonenal hydroxynonenal NOUN ct-9406 260 26 that that PRON ct-9406 260 27 occur occur VERB ct-9406 260 28 during during ADP ct-9406 260 29 cryopreservation cryopreservation NOUN ct-9406 260 30 of of ADP ct-9406 260 31 stallion stallion NOUN ct-9406 260 32 spermatozoa spermatozoa NOUN ct-9406 260 33 lead lead NOUN ct-9406 260 34 to to ADP ct-9406 260 35 caspase caspase NOUN ct-9406 260 36 activation activation NOUN ct-9406 260 37 , , PUNCT ct-9406 260 38 loss loss NOUN ct-9406 260 39 of of ADP ct-9406 260 40 motility motility NOUN ct-9406 260 41 , , PUNCT ct-9406 260 42 and and CCONJ ct-9406 260 43 cell cell NOUN ct-9406 260 44 death death NOUN ct-9406 260 45 . . PUNCT ct-9406 261 1 biol biol PROPN ct-9406 261 2 reprod reprod PROPN ct-9406 261 3 2015;93:143 2015;93:143 PROPN ct-9406 261 4 . . PUNCT ct-9406 262 1 doi doi NOUN ct-9406 262 2 : : PUNCT ct-9406 262 3 10.1095 10.1095 NUM ct-9406 262 4 / / SYM ct-9406 262 5 biolreprod.115.132878 biolreprod.115.132878 ADJ ct-9406 262 6 55 55 NUM ct-9406 262 7 . . PUNCT ct-9406 263 1 gallardo gallardo PROPN ct-9406 263 2 bjm bjm PROPN ct-9406 263 3 , , PUNCT ct-9406 263 4 da da PROPN ct-9406 263 5 silva silva PROPN ct-9406 263 6 bcm bcm PROPN ct-9406 263 7 , , PUNCT ct-9406 263 8 martin martin PROPN ct-9406 263 9 mp mp PROPN ct-9406 263 10 , , PUNCT ct-9406 263 11 et et PROPN ct-9406 263 12 al al PROPN ct-9406 263 13 : : PUNCT ct-9406 263 14 caspase caspase NOUN ct-9406 263 15 activation activation NOUN ct-9406 263 16 , , PUNCT ct-9406 263 17 hydrogen hydrogen NOUN ct-9406 263 18 peroxide peroxide NOUN ct-9406 263 19 production production NOUN ct-9406 263 20 and and CCONJ ct-9406 263 21 akt akt PROPN ct-9406 263 22 dephosphorylation dephosphorylation NOUN ct-9406 263 23 occur occur VERB ct-9406 263 24 during during ADP ct-9406 263 25 stallion stallion NOUN ct-9406 263 26 sperm sperm NOUN ct-9406 263 27 senescence senescence NOUN ct-9406 263 28 . . PUNCT ct-9406 264 1 reprod reprod PROPN ct-9406 264 2 domest domest PROPN ct-9406 264 3 anim anim PROPN ct-9406 264 4 2014;49:657–664 2014;49:657–664 NUM ct-9406 264 5 . . PUNCT ct-9406 265 1 doi doi NOUN ct-9406 265 2 : : PUNCT ct-9406 265 3 10.1111 10.1111 NUM ct-9406 265 4 / / SYM ct-9406 265 5 rda.12343 rda.12343 NUM ct-9406 265 6 56 56 NUM ct-9406 265 7 . . PUNCT ct-9406 266 1 caselles caselles PROPN ct-9406 266 2 ab ab PROPN ct-9406 266 3 , , PUNCT ct-9406 266 4 miro miro PROPN ct-9406 266 5 - - PROPN ct-9406 266 6 moran moran PROPN ct-9406 266 7 a a PROPN ct-9406 266 8 , , PUNCT ct-9406 266 9 morillo morillo PROPN ct-9406 266 10 rodriguez rodriguez PROPN ct-9406 266 11 a a PROPN ct-9406 266 12 , , PUNCT ct-9406 266 13 et et PROPN ct-9406 266 14 al al PROPN ct-9406 266 15 : : PUNCT ct-9406 266 16 identification identification NOUN ct-9406 266 17 of of ADP ct-9406 266 18 apoptotic apoptotic ADJ ct-9406 266 19 bodies body NOUN ct-9406 266 20 in in ADP ct-9406 266 21 equine equine NOUN ct-9406 266 22 semen semen NOUN ct-9406 266 23 . . PUNCT ct-9406 267 1 reprod reprod PROPN ct-9406 267 2 domest domest PROPN ct-9406 267 3 anim anim PROPN ct-9406 267 4 2014;49:254–262 2014;49:254–262 PROPN ct-9406 267 5 . . PUNCT ct-9406 268 1 doi doi NOUN ct-9406 268 2 : : PUNCT ct-9406 268 3 10.1111 10.1111 NUM ct-9406 268 4 / / SYM ct-9406 268 5 rda.12264 rda.12264 NUM ct-9406 268 6 57 57 NUM ct-9406 268 7 . . PUNCT ct-9406 269 1 ortega ortega PROPN ct-9406 269 2 fc fc PROPN ct-9406 269 3 , , PUNCT ct-9406 269 4 gonzalez gonzalez PROPN ct-9406 269 5 fl fl PROPN ct-9406 269 6 , , PUNCT ct-9406 269 7 salazar salazar PROPN ct-9406 269 8 sc sc PROPN ct-9406 269 9 , , PUNCT ct-9406 269 10 et et PROPN ct-9406 269 11 al al PROPN ct-9406 269 12 : : PUNCT ct-9406 269 13 inhibition inhibition NOUN ct-9406 269 14 of of ADP ct-9406 269 15 the the DET ct-9406 269 16 mitochondrial mitochondrial ADJ ct-9406 269 17 permeability permeability NOUN ct-9406 269 18 transition transition NOUN ct-9406 269 19 pore pore NOUN ct-9406 269 20 reduces reduce VERB ct-9406 269 21 “ " PUNCT ct-9406 269 22 apoptosis apoptosis NOUN ct-9406 269 23 like like ADP ct-9406 269 24 ” " PUNCT ct-9406 269 25 changes change NOUN ct-9406 269 26 during during ADP ct-9406 269 27 cryopreservation cryopreservation NOUN ct-9406 269 28 of of ADP ct-9406 269 29 stallion stallion NOUN ct-9406 269 30 sperm sperm NOUN ct-9406 269 31 . . PUNCT ct-9406 270 1 theriogenology theriogenology NOUN ct-9406 270 2 2010;74:458–465 2010;74:458–465 PROPN ct-9406 270 3 . . PUNCT ct-9406 271 1 doi doi NOUN ct-9406 271 2 : : PUNCT ct-9406 271 3 10.1016 10.1016 NUM ct-9406 271 4 / / SYM ct-9406 271 5 j.theriogenology.2010.02.029 j.theriogenology.2010.02.029 PROPN ct-9406 271 6 http://dx.doi.org/10.58292/ct.v15.9406 http://dx.doi.org/10.58292/ct.v15.9406 VERB ct-9406 271 7 https://doi.org/10.1016/0165-0270(85)90068-8 https://doi.org/10.1016/0165-0270(85)90068-8 SCONJ ct-9406 271 8 https://doi.org/10.1530/rep-16-0539 https://doi.org/10.1530/rep-16-0539 PROPN ct-9406 271 9 https://doi.org/10.1071/rd15325 https://doi.org/10.1071/rd15325 PROPN ct-9406 271 10 https://doi.org/10.1042/bj20110114 https://doi.org/10.1042/bj20110114 VERB ct-9406 271 11 https://doi.org/10.1095/biolreprod.116.139444 https://doi.org/10.1095/biolreprod.116.139444 PROPN ct-9406 271 12 https://doi.org/10.1007/s13105-020-00740-6 https://doi.org/10.1007/s13105-020-00740-6 PROPN ct-9406 271 13 https://doi.org/10.1095/biolreprod.115.132878 https://doi.org/10.1095/biolreprod.115.132878 PROPN ct-9406 271 14 https://doi.org/10.1111/rda.12343 https://doi.org/10.1111/rda.12343 PROPN ct-9406 271 15 https://doi.org/10.1111/rda.12264 https://doi.org/10.1111/rda.12264 PROPN ct-9406 271 16 https://doi.org/10.1016/j.theriogenology.2010.02.029 https://doi.org/10.1016/j.theriogenology.2010.02.029 PROPN