id sid tid token lemma pos ct-9899 1 1 semen semen NOUN ct-9899 1 2 extender extender NOUN ct-9899 1 3 type type NOUN ct-9899 1 4 and and CCONJ ct-9899 1 5 cold cold ADJ ct-9899 1 6 storage storage NOUN ct-9899 1 7 of of ADP ct-9899 1 8 tissue tissue NOUN ct-9899 1 9 affect affect VERB ct-9899 1 10 cryosurvival cryosurvival NOUN ct-9899 1 11 of of ADP ct-9899 1 12 alpaca alpaca NOUN ct-9899 1 13 epididymal epididymal ADJ ct-9899 1 14 sperm sperm NOUN ct-9899 1 15 semen semen NOUN ct-9899 1 16 extender extender NOUN ct-9899 1 17 type type NOUN ct-9899 1 18 and and CCONJ ct-9899 1 19 cold cold ADJ ct-9899 1 20 storage storage NOUN ct-9899 1 21 of of ADP ct-9899 1 22 tissue tissue NOUN ct-9899 1 23 affect affect VERB ct-9899 1 24 cryosurvival cryosurvival NOUN ct-9899 1 25 of of ADP ct-9899 1 26 alpaca alpaca NOUN ct-9899 1 27 epididymal epididymal ADJ ct-9899 1 28 sperm sperm NOUN ct-9899 1 29 maria maria PROPN ct-9899 1 30 s. s. PROPN ct-9899 1 31 ferrer ferrer PROPN ct-9899 1 32 , , PUNCT ct-9899 1 33 a a DET ct-9899 1 34 lisa lisa PROPN ct-9899 1 35 williamson williamson PROPN ct-9899 1 36 , , PUNCT ct-9899 1 37 a a DET ct-9899 1 38 roberto roberto PROPN ct-9899 1 39 a. a. NOUN ct-9899 1 40 palomares palomare NOUN ct-9899 1 41 , , PUNCT ct-9899 1 42 b b PROPN ct-9899 1 43 david david PROPN ct-9899 1 44 j. j. PROPN ct-9899 1 45 hurley hurley PROPN ct-9899 1 46 , , PUNCT ct-9899 1 47 b b PROPN ct-9899 1 48 alejandro alejandro PROPN ct-9899 1 49 hoyosjaramillo hoyosjaramillo PROPN ct-9899 1 50 , , PUNCT ct-9899 1 51 b b PROPN ct-9899 1 52 jose jose PROPN ct-9899 1 53 urdanetab urdanetab PROPN ct-9899 1 54 adepartment adepartment NOUN ct-9899 1 55 of of ADP ct-9899 1 56 large large ADJ ct-9899 1 57 animal animal NOUN ct-9899 1 58 medicine medicine NOUN ct-9899 1 59 and and CCONJ ct-9899 1 60 bpopulation bpopulation NOUN ct-9899 1 61 health health NOUN ct-9899 1 62 , , PUNCT ct-9899 1 63 college college NOUN ct-9899 1 64 of of ADP ct-9899 1 65 veterinary veterinary ADJ ct-9899 1 66 medicine medicine NOUN ct-9899 1 67 university university PROPN ct-9899 1 68 of of ADP ct-9899 1 69 georgia georgia PROPN ct-9899 1 70 , , PUNCT ct-9899 1 71 athens athens PROPN ct-9899 1 72 , , PUNCT ct-9899 1 73 ga ga PROPN ct-9899 1 74 abstract abstract NOUN ct-9899 1 75 the the DET ct-9899 1 76 objectives objective NOUN ct-9899 1 77 of of ADP ct-9899 1 78 this this DET ct-9899 1 79 study study NOUN ct-9899 1 80 were be AUX ct-9899 1 81 to to PART ct-9899 1 82 determine determine VERB ct-9899 1 83 the the DET ct-9899 1 84 ability ability NOUN ct-9899 1 85 of of ADP ct-9899 1 86 liposomeand liposomeand VERB ct-9899 1 87 dmfa dmfa NOUN ct-9899 1 88 - - PUNCT ct-9899 1 89 containing contain VERB ct-9899 1 90 semen semen NOUN ct-9899 1 91 extenders extender NOUN ct-9899 1 92 to to PART ct-9899 1 93 support support VERB ct-9899 1 94 cryosurvival cryosurvival NOUN ct-9899 1 95 of of ADP ct-9899 1 96 alpaca alpaca NOUN ct-9899 1 97 epididymal epididymal ADJ ct-9899 1 98 sperm sperm NOUN ct-9899 1 99 , , PUNCT ct-9899 1 100 and and CCONJ ct-9899 1 101 to to PART ct-9899 1 102 evaluate evaluate VERB ct-9899 1 103 the the DET ct-9899 1 104 effect effect NOUN ct-9899 1 105 of of ADP ct-9899 1 106 epididymal epididymal ADJ ct-9899 1 107 cold cold ADJ ct-9899 1 108 storage storage NOUN ct-9899 1 109 on on ADP ct-9899 1 110 post post ADJ ct-9899 1 111 - - ADJ ct-9899 1 112 thaw thaw ADJ ct-9899 1 113 viability viability NOUN ct-9899 1 114 . . PUNCT ct-9899 2 1 it it PRON ct-9899 2 2 was be AUX ct-9899 2 3 hypothesized hypothesize VERB ct-9899 2 4 that that SCONJ ct-9899 2 5 both both DET ct-9899 2 6 semen semen NOUN ct-9899 2 7 extenders extender NOUN ct-9899 2 8 provide provide VERB ct-9899 2 9 appropriate appropriate ADJ ct-9899 2 10 cryoprotection cryoprotection NOUN ct-9899 2 11 , , PUNCT ct-9899 2 12 and and CCONJ ct-9899 2 13 post post ADJ ct-9899 2 14 - - ADJ ct-9899 2 15 thaw thaw ADJ ct-9899 2 16 sperm sperm NOUN ct-9899 2 17 viability viability NOUN ct-9899 2 18 does do AUX ct-9899 2 19 not not PART ct-9899 2 20 differ differ VERB ct-9899 2 21 when when SCONJ ct-9899 2 22 alpaca alpaca NOUN ct-9899 2 23 epididymal epididymal ADJ ct-9899 2 24 sperm sperm NOUN ct-9899 2 25 are be AUX ct-9899 2 26 cryopreserved cryopreserve VERB ct-9899 2 27 immediately immediately ADV ct-9899 2 28 after after ADP ct-9899 2 29 castration castration NOUN ct-9899 2 30 or or CCONJ ct-9899 2 31 after after ADP ct-9899 2 32 24 24 NUM ct-9899 2 33 h h NOUN ct-9899 2 34 of of ADP ct-9899 2 35 cold cold ADJ ct-9899 2 36 storage storage NOUN ct-9899 2 37 . . PUNCT ct-9899 3 1 sperm sperm NOUN ct-9899 3 2 were be AUX ct-9899 3 3 recovered recover VERB ct-9899 3 4 from from ADP ct-9899 3 5 one one NUM ct-9899 3 6 epididymis epididymis NOUN ct-9899 3 7 from from ADP ct-9899 3 8 each each DET ct-9899 3 9 male male NOUN ct-9899 3 10 ( ( PUNCT ct-9899 3 11 n n NOUN ct-9899 3 12 = = SYM ct-9899 3 13 10 10 NUM ct-9899 3 14 ) ) PUNCT ct-9899 3 15 on on ADP ct-9899 3 16 the the DET ct-9899 3 17 day day NOUN ct-9899 3 18 of of ADP ct-9899 3 19 castration castration NOUN ct-9899 3 20 , , PUNCT ct-9899 3 21 and and CCONJ ct-9899 3 22 from from ADP ct-9899 3 23 the the DET ct-9899 3 24 other other ADJ ct-9899 3 25 one one NUM ct-9899 3 26 after after ADP ct-9899 3 27 24 24 NUM ct-9899 3 28 h h NOUN ct-9899 3 29 of of ADP ct-9899 3 30 tissue tissue NOUN ct-9899 3 31 storage storage NOUN ct-9899 3 32 at at ADP ct-9899 3 33 4c 4c PROPN ct-9899 3 34 . . PUNCT ct-9899 4 1 on on ADP ct-9899 4 2 each each DET ct-9899 4 3 day day NOUN ct-9899 4 4 , , PUNCT ct-9899 4 5 each each DET ct-9899 4 6 sample sample NOUN ct-9899 4 7 was be AUX ct-9899 4 8 divided divide VERB ct-9899 4 9 into into ADP ct-9899 4 10 two two NUM ct-9899 4 11 aliquots aliquot NOUN ct-9899 4 12 , , PUNCT ct-9899 4 13 which which PRON ct-9899 4 14 were be AUX ct-9899 4 15 cryopreserved cryopreserve VERB ct-9899 4 16 in in ADP ct-9899 4 17 semen semen NOUN ct-9899 4 18 extender extender NOUN ct-9899 4 19 optixcell optixcell NOUN ct-9899 4 20 or or CCONJ ct-9899 4 21 botucrio botucrio NOUN ct-9899 4 22 . . PUNCT ct-9899 5 1 pre pre ADJ ct-9899 5 2 - - ADJ ct-9899 5 3 freezing freezing ADJ ct-9899 5 4 sperm sperm NOUN ct-9899 5 5 parameters parameter NOUN ct-9899 5 6 did do AUX ct-9899 5 7 not not PART ct-9899 5 8 differ differ VERB ct-9899 5 9 between between ADP ct-9899 5 10 samples sample NOUN ct-9899 5 11 obtained obtain VERB ct-9899 5 12 on on ADP ct-9899 5 13 the the DET ct-9899 5 14 day day NOUN ct-9899 5 15 of of ADP ct-9899 5 16 castration castration NOUN ct-9899 5 17 or or CCONJ ct-9899 5 18 24 24 NUM ct-9899 5 19 h h NOUN ct-9899 5 20 later later ADV ct-9899 5 21 . . PUNCT ct-9899 6 1 motility motility NOUN ct-9899 6 2 of of ADP ct-9899 6 3 sperm sperm NOUN ct-9899 6 4 cryopreserved cryopreserve VERB ct-9899 6 5 in in ADP ct-9899 6 6 botucrio botucrio NOUN ct-9899 6 7 on on ADP ct-9899 6 8 the the DET ct-9899 6 9 day day NOUN ct-9899 6 10 of of ADP ct-9899 6 11 castration castration NOUN ct-9899 6 12 did do AUX ct-9899 6 13 not not PART ct-9899 6 14 differ differ VERB ct-9899 6 15 from from ADP ct-9899 6 16 pre pre ADJ ct-9899 6 17 - - ADJ ct-9899 6 18 freezing freezing ADJ ct-9899 6 19 values value NOUN ct-9899 6 20 . . PUNCT ct-9899 7 1 use use NOUN ct-9899 7 2 of of ADP ct-9899 7 3 optixcell optixcell NUM ct-9899 7 4 or or CCONJ ct-9899 7 5 delaying delay VERB ct-9899 7 6 sperm sperm NOUN ct-9899 7 7 cryopreservation cryopreservation NOUN ct-9899 7 8 decreased decrease VERB ct-9899 7 9 total total NOUN ct-9899 7 10 ( ( PUNCT ct-9899 7 11 p p X ct-9899 7 12 = = NOUN ct-9899 7 13 0.002 0.002 NUM ct-9899 7 14 ) ) PUNCT ct-9899 7 15 and and CCONJ ct-9899 7 16 progressive progressive ADJ ct-9899 7 17 sperm sperm NOUN ct-9899 7 18 motility motility NOUN ct-9899 7 19 ( ( PUNCT ct-9899 7 20 p p X ct-9899 7 21 = = NOUN ct-9899 7 22 0.041 0.041 NUM ct-9899 7 23 ) ) PUNCT ct-9899 7 24 compared compare VERB ct-9899 7 25 with with ADP ct-9899 7 26 pre pre ADJ ct-9899 7 27 - - ADJ ct-9899 7 28 freezing freezing ADJ ct-9899 7 29 values value NOUN ct-9899 7 30 seen see VERB ct-9899 7 31 in in ADP ct-9899 7 32 sperm sperm NOUN ct-9899 7 33 recovered recover VERB ct-9899 7 34 on on ADP ct-9899 7 35 the the DET ct-9899 7 36 day day NOUN ct-9899 7 37 of of ADP ct-9899 7 38 castration castration NOUN ct-9899 7 39 . . PUNCT ct-9899 8 1 membrane membrane NOUN ct-9899 8 2 integrity integrity NOUN ct-9899 8 3 was be AUX ct-9899 8 4 lowest low ADJ ct-9899 8 5 when when SCONJ ct-9899 8 6 sperm sperm NOUN ct-9899 8 7 were be AUX ct-9899 8 8 cryopreserved cryopreserve VERB ct-9899 8 9 in in ADP ct-9899 8 10 optixcell optixcell NOUN ct-9899 8 11 after after ADP ct-9899 8 12 cold cold ADJ ct-9899 8 13 storage storage NOUN ct-9899 8 14 . . PUNCT ct-9899 9 1 treatment treatment NOUN ct-9899 9 2 had have VERB ct-9899 9 3 no no DET ct-9899 9 4 effect effect NOUN ct-9899 9 5 on on ADP ct-9899 9 6 dna dna PROPN ct-9899 9 7 integrity integrity NOUN ct-9899 9 8 . . PUNCT ct-9899 10 1 it it PRON ct-9899 10 2 was be AUX ct-9899 10 3 concluded conclude VERB ct-9899 10 4 that that SCONJ ct-9899 10 5 the the DET ct-9899 10 6 dmfa dmfa NOUN ct-9899 10 7 - - PUNCT ct-9899 10 8 containing contain VERB ct-9899 10 9 semen semen NOUN ct-9899 10 10 extender extender NOUN ct-9899 10 11 botucrio botucrio NOUN ct-9899 10 12 was be AUX ct-9899 10 13 superior superior ADJ ct-9899 10 14 to to ADP ct-9899 10 15 the the DET ct-9899 10 16 liposome liposome NOUN ct-9899 10 17 - - PUNCT ct-9899 10 18 containing contain VERB ct-9899 10 19 semen semen NOUN ct-9899 10 20 extender extender NOUN ct-9899 10 21 optixcell optixcell NOUN ct-9899 10 22 at at ADP ct-9899 10 23 preserving preserve VERB ct-9899 10 24 post post ADJ ct-9899 10 25 - - ADJ ct-9899 10 26 thaw thaw ADJ ct-9899 10 27 viability viability NOUN ct-9899 10 28 of of ADP ct-9899 10 29 alpaca alpaca NOUN ct-9899 10 30 epididymal epididymal ADJ ct-9899 10 31 sperm sperm NOUN ct-9899 10 32 . . PUNCT ct-9899 11 1 cold cold ADJ ct-9899 11 2 storage storage NOUN ct-9899 11 3 of of ADP ct-9899 11 4 epididymides epididymis NOUN ct-9899 11 5 for for ADP ct-9899 11 6 24 24 NUM ct-9899 11 7 h h NOUN ct-9899 11 8 negatively negatively ADV ct-9899 11 9 affected affect VERB ct-9899 11 10 post post ADJ ct-9899 11 11 - - ADJ ct-9899 11 12 thaw thaw ADJ ct-9899 11 13 motility motility NOUN ct-9899 11 14 and and CCONJ ct-9899 11 15 membrane membrane NOUN ct-9899 11 16 integrity integrity NOUN ct-9899 11 17 of of ADP ct-9899 11 18 sperm sperm NOUN ct-9899 11 19 . . PUNCT ct-9899 12 1 therefore therefore ADV ct-9899 12 2 , , PUNCT ct-9899 12 3 cryopreservation cryopreservation NOUN ct-9899 12 4 with with ADP ct-9899 12 5 botucrio botucrio NOUN ct-9899 12 6 immediately immediately ADV ct-9899 12 7 after after SCONJ ct-9899 12 8 castration castration NOUN ct-9899 12 9 is be AUX ct-9899 12 10 recommended recommend VERB ct-9899 12 11 for for ADP ct-9899 12 12 best good ADJ ct-9899 12 13 preservation preservation NOUN ct-9899 12 14 of of ADP ct-9899 12 15 post post ADJ ct-9899 12 16 - - ADJ ct-9899 12 17 thaw thaw ADJ ct-9899 12 18 viability viability NOUN ct-9899 12 19 of of ADP ct-9899 12 20 alpaca alpaca NOUN ct-9899 12 21 epididymal epididymal ADJ ct-9899 12 22 sperm sperm NOUN ct-9899 12 23 . . PUNCT ct-9899 13 1 keywords keyword NOUN ct-9899 13 2 : : PUNCT ct-9899 13 3 camelid camelid ADJ ct-9899 13 4 , , PUNCT ct-9899 13 5 male male NOUN ct-9899 13 6 , , PUNCT ct-9899 13 7 diluent diluent NOUN ct-9899 13 8 , , PUNCT ct-9899 13 9 freezing freezing NOUN ct-9899 13 10 , , PUNCT ct-9899 13 11 post post ADJ ct-9899 13 12 - - ADJ ct-9899 13 13 thaw thaw ADJ ct-9899 13 14 introduction introduction NOUN ct-9899 13 15 harvest harvest NOUN ct-9899 13 16 and and CCONJ ct-9899 13 17 cryopreservation cryopreservation NOUN ct-9899 13 18 of of ADP ct-9899 13 19 epididymal epididymal ADJ ct-9899 13 20 sperm sperm NOUN ct-9899 13 21 enables enable VERB ct-9899 13 22 preservation preservation NOUN ct-9899 13 23 of of ADP ct-9899 13 24 genetic genetic ADJ ct-9899 13 25 material material NOUN ct-9899 13 26 in in ADP ct-9899 13 27 the the DET ct-9899 13 28 event event NOUN ct-9899 13 29 of of ADP ct-9899 13 30 unexpected unexpected ADJ ct-9899 13 31 death death NOUN ct-9899 13 32 of of ADP ct-9899 13 33 an an DET ct-9899 13 34 animal animal NOUN ct-9899 13 35 . . PUNCT ct-9899 14 1 while while SCONJ ct-9899 14 2 reports report NOUN ct-9899 14 3 of of ADP ct-9899 14 4 this this DET ct-9899 14 5 technique technique NOUN ct-9899 14 6 exist exist VERB ct-9899 14 7 in in ADP ct-9899 14 8 camelids camelid NOUN ct-9899 14 9 , , PUNCT ct-9899 14 10 an an DET ct-9899 14 11 efficient efficient ADJ ct-9899 14 12 protocol protocol NOUN ct-9899 14 13 for for ADP ct-9899 14 14 epididymal epididymal ADJ ct-9899 14 15 sperm sperm NOUN ct-9899 14 16 cryopreservation cryopreservation NOUN ct-9899 14 17 has have AUX ct-9899 14 18 not not PART ct-9899 14 19 been be AUX ct-9899 14 20 developed develop VERB ct-9899 14 21 . . PUNCT ct-9899 15 1 initial initial ADJ ct-9899 15 2 reports report NOUN ct-9899 15 3 have have AUX ct-9899 15 4 shown show VERB ct-9899 15 5 that that SCONJ ct-9899 15 6 lactose lactose NOUN ct-9899 15 7 - - PUNCT ct-9899 15 8 based base VERB ct-9899 15 9 semen semen NOUN ct-9899 15 10 extenders extender NOUN ct-9899 15 11 provided provide VERB ct-9899 15 12 better well ADJ ct-9899 15 13 post post ADJ ct-9899 15 14 - - ADJ ct-9899 15 15 thaw thaw ADJ ct-9899 15 16 motility motility NOUN ct-9899 15 17 than than ADP ct-9899 15 18 citrateor citrateor PROPN ct-9899 15 19 tris tris NOUN ct-9899 15 20 - - PUNCT ct-9899 15 21 based base VERB ct-9899 15 22 extenders.1 extenders.1 PROPN ct-9899 15 23 using use VERB ct-9899 15 24 lactose lactose NOUN ct-9899 15 25 - - PUNCT ct-9899 15 26 based base VERB ct-9899 15 27 extenders extender NOUN ct-9899 15 28 , , PUNCT ct-9899 15 29 it it PRON ct-9899 15 30 was be AUX ct-9899 15 31 then then ADV ct-9899 15 32 shown show VERB ct-9899 15 33 that that SCONJ ct-9899 15 34 final final ADJ ct-9899 15 35 concentrations concentration NOUN ct-9899 15 36 of of ADP ct-9899 15 37 3 3 NUM ct-9899 15 38 to to PART ct-9899 15 39 4 4 NUM ct-9899 15 40 % % NOUN ct-9899 15 41 glycerol glycerol NOUN ct-9899 15 42 and and CCONJ ct-9899 15 43 1 1 NUM ct-9899 15 44 % % NOUN ct-9899 15 45 equex equex NOUN ct-9899 15 46 stm stm PROPN ct-9899 15 47 paste paste PROPN ct-9899 15 48 provided provide VERB ct-9899 15 49 ideal ideal NOUN ct-9899 15 50 cryoprotection.2 cryoprotection.2 PROPN ct-9899 15 51 however however ADV ct-9899 15 52 , , PUNCT ct-9899 15 53 mean mean VERB ct-9899 15 54 post post ADJ ct-9899 15 55 - - ADJ ct-9899 15 56 thaw thaw ADJ ct-9899 15 57 motility motility NOUN ct-9899 15 58 was be AUX ct-9899 15 59 low low ADJ ct-9899 15 60 , , PUNCT ct-9899 15 61 ranging range VERB ct-9899 15 62 from from ADP ct-9899 15 63 14.4 14.4 NUM ct-9899 15 64 to to ADP ct-9899 15 65 27%.1,2 27%.1,2 NUM ct-9899 15 66 later later ADJ ct-9899 15 67 work work NOUN ct-9899 15 68 testing test VERB ct-9899 15 69 alternative alternative ADJ ct-9899 15 70 cryprotectants cryprotectant NOUN ct-9899 15 71 demonstrated demonstrate VERB ct-9899 15 72 that that SCONJ ct-9899 15 73 post post ADJ ct-9899 15 74 - - ADJ ct-9899 15 75 thaw thaw ADJ ct-9899 15 76 motility motility NOUN ct-9899 15 77 was be AUX ct-9899 15 78 improved improve VERB ct-9899 15 79 when when SCONJ ct-9899 15 80 dimethylsulfoxide dimethylsulfoxide ADJ ct-9899 15 81 ( ( PUNCT ct-9899 15 82 dmso dmso NOUN ct-9899 15 83 ) ) PUNCT ct-9899 15 84 or or CCONJ ct-9899 15 85 diacetylamide diacetylamide ADV ct-9899 15 86 ( ( PUNCT ct-9899 15 87 dma dma NOUN ct-9899 15 88 ) ) PUNCT ct-9899 15 89 were be AUX ct-9899 15 90 used use VERB ct-9899 15 91 instead instead ADV ct-9899 15 92 of of ADP ct-9899 15 93 ethylene ethylene NOUN ct-9899 15 94 glycol glycol NOUN ct-9899 15 95 or or CCONJ ct-9899 15 96 glycerol.3,4 glycerol.3,4 ADJ ct-9899 15 97 with with ADP ct-9899 15 98 addition addition NOUN ct-9899 15 99 of of ADP ct-9899 15 100 these these DET ct-9899 15 101 cryoprotectants cryoprotectant NOUN ct-9899 15 102 , , PUNCT ct-9899 15 103 mean mean VERB ct-9899 15 104 post post ADJ ct-9899 15 105 - - ADJ ct-9899 15 106 thaw thaw ADJ ct-9899 15 107 motility motility NOUN ct-9899 15 108 ranged range VERB ct-9899 15 109 from from ADP ct-9899 15 110 31 31 NUM ct-9899 15 111 to to ADP ct-9899 15 112 34%.3,4 34%.3,4 NUM ct-9899 15 113 amides amide NOUN ct-9899 15 114 , , PUNCT ct-9899 15 115 like like ADP ct-9899 15 116 dma dma NOUN ct-9899 15 117 and and CCONJ ct-9899 15 118 dimethylformamide dimethylformamide NOUN ct-9899 15 119 ( ( PUNCT ct-9899 15 120 dmfa dmfa NOUN ct-9899 15 121 ) ) PUNCT ct-9899 15 122 are be AUX ct-9899 15 123 small small ADJ ct-9899 15 124 hydrophilic hydrophilic ADJ ct-9899 15 125 molecules molecule NOUN ct-9899 15 126 with with ADP ct-9899 15 127 better well ADJ ct-9899 15 128 cell cell NOUN ct-9899 15 129 penetration penetration NOUN ct-9899 15 130 and and CCONJ ct-9899 15 131 less less ADJ ct-9899 15 132 toxicity toxicity NOUN ct-9899 15 133 than than SCONJ ct-9899 15 134 glycerol.4,5 glycerol.4,5 NOUN ct-9899 15 135 adding add VERB ct-9899 15 136 dmfa dmfa NOUN ct-9899 15 137 to to ADP ct-9899 15 138 the the DET ct-9899 15 139 semen semen NOUN ct-9899 15 140 extender extender NOUN ct-9899 15 141 improved improve VERB ct-9899 15 142 cryosurvival cryosurvival NOUN ct-9899 15 143 of of ADP ct-9899 15 144 stallion stallion NOUN ct-9899 15 145 epididymal epididymal ADJ ct-9899 15 146 sperm.6 sperm.6 PROPN ct-9899 15 147 in in ADP ct-9899 15 148 bulls bull NOUN ct-9899 15 149 , , PUNCT ct-9899 15 150 addition addition NOUN ct-9899 15 151 of of ADP ct-9899 15 152 liposomes liposome NOUN ct-9899 15 153 improved improve VERB ct-9899 15 154 post post ADJ ct-9899 15 155 - - ADJ ct-9899 15 156 thaw thaw ADJ ct-9899 15 157 semen semen NOUN ct-9899 15 158 quality.7,8 quality.7,8 ADV ct-9899 15 159 liposomes liposome NOUN ct-9899 15 160 can can AUX ct-9899 15 161 incorporate incorporate VERB ct-9899 15 162 into into ADP ct-9899 15 163 the the DET ct-9899 15 164 plasma plasma NOUN ct-9899 15 165 membrane membrane NOUN ct-9899 15 166 and and CCONJ ct-9899 15 167 change change VERB ct-9899 15 168 the the DET ct-9899 15 169 lipid lipid NOUN ct-9899 15 170 phase phase NOUN ct-9899 15 171 transition transition NOUN ct-9899 15 172 , , PUNCT ct-9899 15 173 decreasing decrease VERB ct-9899 15 174 the the DET ct-9899 15 175 sensitivity sensitivity NOUN ct-9899 15 176 of of ADP ct-9899 15 177 sperm sperm NOUN ct-9899 15 178 to to PART ct-9899 15 179 low low VERB ct-9899 15 180 temperatures.7 temperatures.7 NOUN ct-9899 15 181 the the DET ct-9899 15 182 use use NOUN ct-9899 15 183 of of ADP ct-9899 15 184 liposome liposome NOUN ct-9899 15 185 and and CCONJ ct-9899 15 186 dmfa dmfa NOUN ct-9899 15 187 containing contain VERB ct-9899 15 188 commercial commercial ADJ ct-9899 15 189 semen semen NOUN ct-9899 15 190 extenders extender NOUN ct-9899 15 191 for for ADP ct-9899 15 192 cryopreservation cryopreservation NOUN ct-9899 15 193 of of ADP ct-9899 15 194 alpaca alpaca NOUN ct-9899 15 195 sperm sperm NOUN ct-9899 15 196 has have AUX ct-9899 15 197 not not PART ct-9899 15 198 been be AUX ct-9899 15 199 yet yet ADV ct-9899 15 200 tested test VERB ct-9899 15 201 . . PUNCT ct-9899 16 1 in in ADP ct-9899 16 2 addition addition NOUN ct-9899 16 3 to to ADP ct-9899 16 4 semen semen NOUN ct-9899 16 5 extenders extender NOUN ct-9899 16 6 , , PUNCT ct-9899 16 7 holding hold VERB ct-9899 16 8 time time NOUN ct-9899 16 9 could could AUX ct-9899 16 10 also also ADV ct-9899 16 11 have have VERB ct-9899 16 12 an an DET ct-9899 16 13 impact impact NOUN ct-9899 16 14 on on ADP ct-9899 16 15 post post ADJ ct-9899 16 16 - - ADJ ct-9899 16 17 thaw thaw ADJ ct-9899 16 18 semen semen NOUN ct-9899 16 19 quality quality NOUN ct-9899 16 20 . . PUNCT ct-9899 17 1 the the DET ct-9899 17 2 ability ability NOUN ct-9899 17 3 to to PART ct-9899 17 4 hold hold VERB ct-9899 17 5 epididymides epididymis NOUN ct-9899 17 6 for for ADP ct-9899 17 7 a a DET ct-9899 17 8 prolonged prolonged ADJ ct-9899 17 9 time time NOUN ct-9899 17 10 allows allow VERB ct-9899 17 11 practitioners practitioner NOUN ct-9899 17 12 to to PART ct-9899 17 13 transport transport VERB ct-9899 17 14 tissue tissue NOUN ct-9899 17 15 to to ADP ct-9899 17 16 the the DET ct-9899 17 17 laboratory laboratory NOUN ct-9899 17 18 for for ADP ct-9899 17 19 further further ADJ ct-9899 17 20 processing processing NOUN ct-9899 17 21 and and CCONJ ct-9899 17 22 cryopreservation cryopreservation NOUN ct-9899 17 23 . . PUNCT ct-9899 18 1 this this DET ct-9899 18 2 practice practice NOUN ct-9899 18 3 is be AUX ct-9899 18 4 routinely routinely ADV ct-9899 18 5 performed perform VERB ct-9899 18 6 in in ADP ct-9899 18 7 stallions stallion NOUN ct-9899 18 8 since since SCONJ ct-9899 18 9 cold cold ADJ ct-9899 18 10 storage storage NOUN ct-9899 18 11 for for SCONJ ct-9899 18 12 up up ADP ct-9899 18 13 to to PART ct-9899 18 14 96 96 NUM ct-9899 18 15 h h NOUN ct-9899 18 16 does do AUX ct-9899 18 17 not not PART ct-9899 18 18 affect affect VERB ct-9899 18 19 quality quality NOUN ct-9899 18 20 or or CCONJ ct-9899 18 21 fertility fertility NOUN ct-9899 18 22 of of ADP ct-9899 18 23 cryopreserved cryopreserved ADJ ct-9899 18 24 equine equine NOUN ct-9899 18 25 sperm.9,10 sperm.9,10 NOUN ct-9899 18 26 in in ADP ct-9899 18 27 spite spite NOUN ct-9899 18 28 of of ADP ct-9899 18 29 this this DET ct-9899 18 30 apparent apparent ADJ ct-9899 18 31 advantage advantage NOUN ct-9899 18 32 , , PUNCT ct-9899 18 33 there there PRON ct-9899 18 34 have have AUX ct-9899 18 35 been be AUX ct-9899 18 36 no no DET ct-9899 18 37 direct direct ADJ ct-9899 18 38 comparisons comparison NOUN ct-9899 18 39 on on ADP ct-9899 18 40 post post ADJ ct-9899 18 41 - - ADJ ct-9899 18 42 thaw thaw ADJ ct-9899 18 43 motility motility NOUN ct-9899 18 44 of of ADP ct-9899 18 45 alpaca alpaca NOUN ct-9899 18 46 epididymal epididymal ADJ ct-9899 18 47 sperm sperm NOUN ct-9899 18 48 cryopreserved cryopreserve VERB ct-9899 18 49 immediately immediately ADV ct-9899 18 50 after after ADP ct-9899 18 51 castration castration NOUN ct-9899 18 52 or or CCONJ ct-9899 18 53 after after ADP ct-9899 18 54 24 24 NUM ct-9899 18 55 h h NOUN ct-9899 18 56 of of ADP ct-9899 18 57 cold cold ADJ ct-9899 18 58 storage storage NOUN ct-9899 18 59 . . PUNCT ct-9899 19 1 419 419 NUM ct-9899 19 2 clinical clinical ADJ ct-9899 19 3 theriogenology theriogenology NOUN ct-9899 19 4 • • NOUN ct-9899 19 5 volume volume NOUN ct-9899 19 6 10 10 NUM ct-9899 19 7 , , PUNCT ct-9899 19 8 number number NOUN ct-9899 19 9 4 4 NUM ct-9899 19 10 • • NOUN ct-9899 19 11 december december PROPN ct-9899 19 12 2018 2018 NUM ct-9899 19 13 the the DET ct-9899 19 14 objectives objective NOUN ct-9899 19 15 of of ADP ct-9899 19 16 this this DET ct-9899 19 17 study study NOUN ct-9899 19 18 were be AUX ct-9899 19 19 to to PART ct-9899 19 20 determine determine VERB ct-9899 19 21 the the DET ct-9899 19 22 ability ability NOUN ct-9899 19 23 of of ADP ct-9899 19 24 commercial commercial ADJ ct-9899 19 25 semen semen NOUN ct-9899 19 26 extenders extender NOUN ct-9899 19 27 containing contain VERB ct-9899 19 28 liposomes liposome NOUN ct-9899 19 29 and and CCONJ ct-9899 19 30 dmfa dmfa NOUN ct-9899 19 31 to to PART ct-9899 19 32 support support VERB ct-9899 19 33 cryosurvival cryosurvival NOUN ct-9899 19 34 of of ADP ct-9899 19 35 alpaca alpaca NOUN ct-9899 19 36 epididymal epididymal ADJ ct-9899 19 37 sperm sperm NOUN ct-9899 19 38 , , PUNCT ct-9899 19 39 and and CCONJ ct-9899 19 40 to to PART ct-9899 19 41 evaluate evaluate VERB ct-9899 19 42 the the DET ct-9899 19 43 effect effect NOUN ct-9899 19 44 of of ADP ct-9899 19 45 cold cold ADJ ct-9899 19 46 storage storage NOUN ct-9899 19 47 on on ADP ct-9899 19 48 post post ADJ ct-9899 19 49 - - ADJ ct-9899 19 50 thaw thaw ADJ ct-9899 19 51 sperm sperm NOUN ct-9899 19 52 parameters parameter NOUN ct-9899 19 53 . . PUNCT ct-9899 20 1 it it PRON ct-9899 20 2 was be AUX ct-9899 20 3 hypothesized hypothesize VERB ct-9899 20 4 that that SCONJ ct-9899 20 5 both both DET ct-9899 20 6 semen semen NOUN ct-9899 20 7 extenders extender NOUN ct-9899 20 8 provide provide VERB ct-9899 20 9 appropriate appropriate ADJ ct-9899 20 10 cryoprotection cryoprotection NOUN ct-9899 20 11 , , PUNCT ct-9899 20 12 and and CCONJ ct-9899 20 13 that that SCONJ ct-9899 20 14 post post ADJ ct-9899 20 15 - - ADJ ct-9899 20 16 thaw thaw ADJ ct-9899 20 17 sperm sperm NOUN ct-9899 20 18 parameters parameter NOUN ct-9899 20 19 do do AUX ct-9899 20 20 not not PART ct-9899 20 21 differ differ VERB ct-9899 20 22 when when SCONJ ct-9899 20 23 alpaca alpaca NOUN ct-9899 20 24 epididymal epididymal ADJ ct-9899 20 25 sperm sperm NOUN ct-9899 20 26 are be AUX ct-9899 20 27 cryopreserved cryopreserve VERB ct-9899 20 28 immediately immediately ADV ct-9899 20 29 after after ADP ct-9899 20 30 castration castration NOUN ct-9899 20 31 or or CCONJ ct-9899 20 32 after after ADP ct-9899 20 33 24 24 NUM ct-9899 20 34 h h NOUN ct-9899 20 35 of of ADP ct-9899 20 36 cold cold ADJ ct-9899 20 37 storage storage NOUN ct-9899 20 38 . . PUNCT ct-9899 21 1 materials material NOUN ct-9899 21 2 and and CCONJ ct-9899 21 3 methods method NOUN ct-9899 21 4 animals animal NOUN ct-9899 21 5 and and CCONJ ct-9899 21 6 castrations castration NOUN ct-9899 21 7 twenty twenty NUM ct-9899 21 8 testes testis NOUN ct-9899 21 9 were be AUX ct-9899 21 10 collected collect VERB ct-9899 21 11 from from ADP ct-9899 21 12 10 10 NUM ct-9899 21 13 male male ADJ ct-9899 21 14 alpacas alpaca NOUN ct-9899 21 15 . . PUNCT ct-9899 22 1 the the DET ct-9899 22 2 alpacas alpacas PROPN ct-9899 22 3 were be AUX ct-9899 22 4 of of ADP ct-9899 22 5 huacaya huacaya NOUN ct-9899 22 6 and and CCONJ ct-9899 22 7 suri suri PROPN ct-9899 22 8 breeds breeds PROPN ct-9899 22 9 , , PUNCT ct-9899 22 10 and and CCONJ ct-9899 22 11 1 1 NUM ct-9899 22 12 to to PART ct-9899 22 13 7 7 NUM ct-9899 22 14 y y PROPN ct-9899 22 15 old old ADJ ct-9899 22 16 . . PUNCT ct-9899 23 1 they they PRON ct-9899 23 2 were be AUX ct-9899 23 3 presented present VERB ct-9899 23 4 to to ADP ct-9899 23 5 the the DET ct-9899 23 6 veterinary veterinary ADJ ct-9899 23 7 medical medical ADJ ct-9899 23 8 center center NOUN ct-9899 23 9 of of ADP ct-9899 23 10 the the DET ct-9899 23 11 university university PROPN ct-9899 23 12 of of ADP ct-9899 23 13 georgia georgia PROPN ct-9899 23 14 for for ADP ct-9899 23 15 elective elective ADJ ct-9899 23 16 castration castration NOUN ct-9899 23 17 . . PUNCT ct-9899 24 1 the the DET ct-9899 24 2 reproductive reproductive ADJ ct-9899 24 3 history history NOUN ct-9899 24 4 of of ADP ct-9899 24 5 these these DET ct-9899 24 6 males male NOUN ct-9899 24 7 was be AUX ct-9899 24 8 not not PART ct-9899 24 9 known know VERB ct-9899 24 10 . . PUNCT ct-9899 25 1 the the DET ct-9899 25 2 alpacas alpaca NOUN ct-9899 25 3 were be AUX ct-9899 25 4 anesthetized anesthetize VERB ct-9899 25 5 with with ADP ct-9899 25 6 xylazine xylazine PROPN ct-9899 25 7 ( ( PUNCT ct-9899 25 8 0.4 0.4 NUM ct-9899 25 9 mg mg PROPN ct-9899 25 10 / / SYM ct-9899 25 11 kg kg PROPN ct-9899 25 12 ) ) PUNCT ct-9899 25 13 , , PUNCT ct-9899 25 14 ketamine ketamine PROPN ct-9899 25 15 ( ( PUNCT ct-9899 25 16 4 4 NUM ct-9899 25 17 mg mg PROPN ct-9899 25 18 / / SYM ct-9899 25 19 kg kg NOUN ct-9899 25 20 ) ) PUNCT ct-9899 25 21 and and CCONJ ct-9899 25 22 butorphanol butorphanol VERB ct-9899 25 23 ( ( PUNCT ct-9899 25 24 0.04 0.04 NUM ct-9899 25 25 mg mg PROPN ct-9899 25 26 / / SYM ct-9899 25 27 kg kg PROPN ct-9899 25 28 ) ) PUNCT ct-9899 25 29 , , PUNCT ct-9899 25 30 all all PRON ct-9899 25 31 of of ADP ct-9899 25 32 them they PRON ct-9899 25 33 given give VERB ct-9899 25 34 intramuscularly intramuscularly ADV ct-9899 25 35 . . PUNCT ct-9899 26 1 the the DET ct-9899 26 2 testes testis NOUN ct-9899 26 3 and and CCONJ ct-9899 26 4 epididymides epididymis NOUN ct-9899 26 5 were be AUX ct-9899 26 6 removed remove VERB ct-9899 26 7 using use VERB ct-9899 26 8 a a DET ct-9899 26 9 standard standard ADJ ct-9899 26 10 closed closed ADJ ct-9899 26 11 castration castration NOUN ct-9899 26 12 technique technique NOUN ct-9899 26 13 . . PUNCT ct-9899 27 1 prior prior ADV ct-9899 27 2 to to ADP ct-9899 27 3 castration castration NOUN ct-9899 27 4 , , PUNCT ct-9899 27 5 the the DET ct-9899 27 6 deferent deferent ADJ ct-9899 27 7 ducts duct NOUN ct-9899 27 8 were be AUX ct-9899 27 9 ligated ligate VERB ct-9899 27 10 with with ADP ct-9899 27 11 2 2 NUM ct-9899 27 12 pds pds NOUN ct-9899 27 13 ii ii NOUN ct-9899 27 14 . . PUNCT ct-9899 28 1 each each DET ct-9899 28 2 pair pair NOUN ct-9899 28 3 of of ADP ct-9899 28 4 testes testis NOUN ct-9899 28 5 with with ADP ct-9899 28 6 epididymides epididymis NOUN ct-9899 28 7 was be AUX ct-9899 28 8 placed place VERB ct-9899 28 9 in in ADP ct-9899 28 10 a a DET ct-9899 28 11 clean clean ADJ ct-9899 28 12 whirlpack whirlpack NOUN ct-9899 28 13 bag bag NOUN ct-9899 28 14 without without ADP ct-9899 28 15 any any DET ct-9899 28 16 addition addition NOUN ct-9899 28 17 of of ADP ct-9899 28 18 media medium NOUN ct-9899 28 19 , , PUNCT ct-9899 28 20 and and CCONJ ct-9899 28 21 was be AUX ct-9899 28 22 placed place VERB ct-9899 28 23 at at ADP ct-9899 28 24 4 4 NUM ct-9899 28 25 ° ° ADP ct-9899 28 26 c c NOUN ct-9899 28 27 in in ADP ct-9899 28 28 the the DET ct-9899 28 29 truck truck NOUN ct-9899 28 30 ’s ’s PART ct-9899 28 31 refrigerator refrigerator NOUN ct-9899 28 32 until until ADP ct-9899 28 33 processing processing NOUN ct-9899 28 34 . . PUNCT ct-9899 29 1 experimental experimental ADJ ct-9899 29 2 design design NOUN ct-9899 29 3 and and CCONJ ct-9899 29 4 sperm sperm NOUN ct-9899 29 5 collection collection NOUN ct-9899 29 6     SPACE ct-9899 29 7 consequent consequent NOUN ct-9899 29 8 to to ADP ct-9899 29 9 arrival arrival NOUN ct-9899 29 10 to to ADP ct-9899 29 11 the the DET ct-9899 29 12 laboratory laboratory NOUN ct-9899 29 13 , , PUNCT ct-9899 29 14 one one NUM ct-9899 29 15 epididymis epididymis NOUN ct-9899 29 16 from from ADP ct-9899 29 17 each each DET ct-9899 29 18 male male NOUN ct-9899 29 19 was be AUX ct-9899 29 20 immediately immediately ADV ct-9899 29 21 processed process VERB ct-9899 29 22 , , PUNCT ct-9899 29 23 while while SCONJ ct-9899 29 24 the the DET ct-9899 29 25 other other ADJ ct-9899 29 26 testis testis NOUN ct-9899 29 27 remained remain VERB ct-9899 29 28 at at ADP ct-9899 29 29 4c 4c PROPN ct-9899 29 30 until until ADP ct-9899 29 31 processing process VERB ct-9899 29 32 the the DET ct-9899 29 33 following following ADJ ct-9899 29 34 day day NOUN ct-9899 29 35 . . PUNCT ct-9899 30 1 tissue tissue NOUN ct-9899 30 2 was be AUX ct-9899 30 3 processed process VERB ct-9899 30 4 as as SCONJ ct-9899 30 5 previously previously ADV ct-9899 30 6 described describe VERB ct-9899 30 7 , , PUNCT ct-9899 30 8 with with ADP ct-9899 30 9 some some DET ct-9899 30 10 modifications.1,2 modifications.1,2 ADJ ct-9899 30 11 the the DET ct-9899 30 12 parietal parietal ADJ ct-9899 30 13 vaginal vaginal ADJ ct-9899 30 14 tunic tunic NOUN ct-9899 30 15 was be AUX ct-9899 30 16 opened open VERB ct-9899 30 17 , , PUNCT ct-9899 30 18 and and CCONJ ct-9899 30 19 the the DET ct-9899 30 20 testes testis NOUN ct-9899 30 21 and and CCONJ ct-9899 30 22 epididymides epididymis NOUN ct-9899 30 23 were be AUX ct-9899 30 24 exposed expose VERB ct-9899 30 25 and and CCONJ ct-9899 30 26 rinsed rinse VERB ct-9899 30 27 with with ADP ct-9899 30 28 0.9 0.9 NUM ct-9899 30 29 % % NOUN ct-9899 30 30 sodium sodium NOUN ct-9899 30 31 chloride chloride NOUN ct-9899 30 32 solution solution NOUN ct-9899 30 33 . . PUNCT ct-9899 31 1 a a DET ct-9899 31 2 ligature ligature NOUN ct-9899 31 3 ( ( PUNCT ct-9899 31 4 2 2 NUM ct-9899 31 5 - - SYM ct-9899 31 6 0 0 NUM ct-9899 31 7 pds pds PROPN ct-9899 31 8 ii ii PROPN ct-9899 31 9 ) ) PUNCT ct-9899 31 10 was be AUX ct-9899 31 11 placed place VERB ct-9899 31 12 at at ADP ct-9899 31 13 the the DET ct-9899 31 14 junction junction NOUN ct-9899 31 15 between between ADP ct-9899 31 16 the the DET ct-9899 31 17 corpus corpus NOUN ct-9899 31 18 and and CCONJ ct-9899 31 19 cauda cauda NOUN ct-9899 31 20 epididymides epididymis NOUN ct-9899 31 21 . . PUNCT ct-9899 32 1 cauda cauda NOUN ct-9899 32 2 epididymides epididymis NOUN ct-9899 32 3 , , PUNCT ct-9899 32 4 including include VERB ct-9899 32 5 at at ADV ct-9899 32 6 least least ADV ct-9899 32 7 1 1 NUM ct-9899 32 8 cm cm NOUN ct-9899 32 9 of of ADP ct-9899 32 10 the the DET ct-9899 32 11 proximal proximal ADJ ct-9899 32 12 ductus ductus NOUN ct-9899 32 13 deferens deferen NOUN ct-9899 32 14 , , PUNCT ct-9899 32 15 were be AUX ct-9899 32 16 separated separate VERB ct-9899 32 17 and and CCONJ ct-9899 32 18 transferred transfer VERB ct-9899 32 19 to to ADP ct-9899 32 20 a a DET ct-9899 32 21 petri petri ADJ ct-9899 32 22 dish dish NOUN ct-9899 32 23 . . PUNCT ct-9899 33 1 connective connective ADJ ct-9899 33 2 tissue tissue NOUN ct-9899 33 3 containing contain VERB ct-9899 33 4 superficial superficial ADJ ct-9899 33 5 blood blood NOUN ct-9899 33 6 vessels vessel NOUN ct-9899 33 7 around around ADP ct-9899 33 8 the the DET ct-9899 33 9 cauda cauda NOUN ct-9899 33 10 epididymis epididymis NOUN ct-9899 33 11 was be AUX ct-9899 33 12 removed remove VERB ct-9899 33 13 by by ADP ct-9899 33 14 dissection dissection NOUN ct-9899 33 15 with with ADP ct-9899 33 16 scissors scissor NOUN ct-9899 33 17 . . PUNCT ct-9899 34 1 the the DET ct-9899 34 2 tissue tissue NOUN ct-9899 34 3 was be AUX ct-9899 34 4 then then ADV ct-9899 34 5 covered cover VERB ct-9899 34 6 with with ADP ct-9899 34 7 fractionated fractionated ADJ ct-9899 34 8 milk milk NOUN ct-9899 34 9 - - PUNCT ct-9899 34 10 based base VERB ct-9899 34 11 semen semen NOUN ct-9899 34 12 extender extender NOUN ct-9899 34 13 ( ( PUNCT ct-9899 34 14 inra96 inra96 PROPN ct-9899 34 15 , , PUNCT ct-9899 34 16 imv imv PROPN ct-9899 34 17 technologies technology NOUN ct-9899 34 18 , , PUNCT ct-9899 34 19 maple maple PROPN ct-9899 34 20 grove grove PROPN ct-9899 34 21 , , PUNCT ct-9899 34 22 mn mn PROPN ct-9899 34 23 , , PUNCT ct-9899 34 24 usa usa PROPN ct-9899 34 25 ) ) PUNCT ct-9899 34 26 , , PUNCT ct-9899 34 27 and and CCONJ ct-9899 34 28 minced mince VERB ct-9899 34 29 into into ADP ct-9899 34 30 small small ADJ ct-9899 34 31 fragments fragment NOUN ct-9899 34 32 with with ADP ct-9899 34 33 a a DET ct-9899 34 34 scalpel scalpel NOUN ct-9899 34 35 . . PUNCT ct-9899 35 1 while while SCONJ ct-9899 35 2 there there PRON ct-9899 35 3 is be VERB ct-9899 35 4 no no DET ct-9899 35 5 information information NOUN ct-9899 35 6 in in ADP ct-9899 35 7 alpacas alpaca NOUN ct-9899 35 8 , , PUNCT ct-9899 35 9 the the DET ct-9899 35 10 use use NOUN ct-9899 35 11 of of ADP ct-9899 35 12 inra96 inra96 PROPN ct-9899 35 13 resulted result VERB ct-9899 35 14 in in ADP ct-9899 35 15 better well ADJ ct-9899 35 16 preservation preservation NOUN ct-9899 35 17 of of ADP ct-9899 35 18 membrane membrane NOUN ct-9899 35 19 integrity integrity NOUN ct-9899 35 20 and and CCONJ ct-9899 35 21 fertility fertility NOUN ct-9899 35 22 of of ADP ct-9899 35 23 liquid liquid NOUN ct-9899 35 24 - - PUNCT ct-9899 35 25 stored store VERB ct-9899 35 26 camel camel NOUN ct-9899 35 27 semen semen NOUN ct-9899 35 28 compared compare VERB ct-9899 35 29 with with ADP ct-9899 35 30 other other ADJ ct-9899 35 31 commercial commercial ADJ ct-9899 35 32 extenders.11 extenders.11 NOUN ct-9899 35 33 sperm sperm NOUN ct-9899 35 34 were be AUX ct-9899 35 35 allowed allow VERB ct-9899 35 36 to to PART ct-9899 35 37 swim swim VERB ct-9899 35 38 up up ADP ct-9899 35 39 into into ADP ct-9899 35 40 the the DET ct-9899 35 41 medium medium NOUN ct-9899 35 42 by by ADP ct-9899 35 43 incubation incubation NOUN ct-9899 35 44 for for ADP ct-9899 35 45 15 15 NUM ct-9899 35 46 min min NOUN ct-9899 35 47 at at ADP ct-9899 35 48 38 38 NUM ct-9899 35 49 ° ° NOUN ct-9899 35 50 c c NOUN ct-9899 35 51 . . PUNCT ct-9899 36 1 after after ADP ct-9899 36 2 incubation incubation NOUN ct-9899 36 3 , , PUNCT ct-9899 36 4 the the DET ct-9899 36 5 tissue tissue NOUN ct-9899 36 6 fragments fragment NOUN ct-9899 36 7 were be AUX ct-9899 36 8 removed remove VERB ct-9899 36 9 with with ADP ct-9899 36 10 forceps forceps NOUN ct-9899 36 11 . . PUNCT ct-9899 37 1 the the DET ct-9899 37 2 medium medium NOUN ct-9899 37 3 containing contain VERB ct-9899 37 4 sperm sperm NOUN ct-9899 37 5 was be AUX ct-9899 37 6 filtered filter VERB ct-9899 37 7 through through ADP ct-9899 37 8 a a DET ct-9899 37 9 stallion stallion NOUN ct-9899 37 10 semen semen NOUN ct-9899 37 11 gel gel NOUN ct-9899 37 12 filter filter NOUN ct-9899 37 13 ( ( PUNCT ct-9899 37 14 max max NOUN ct-9899 37 15 - - PUNCT ct-9899 37 16 flow flow NOUN ct-9899 37 17 semen semen NOUN ct-9899 37 18 gel gel NOUN ct-9899 37 19 filters filter NOUN ct-9899 37 20 , , PUNCT ct-9899 37 21 nasco nasco PROPN ct-9899 37 22 , , PUNCT ct-9899 37 23 ft ft PROPN ct-9899 37 24 . . PROPN ct-9899 37 25 atkinson atkinson PROPN ct-9899 37 26 , , PUNCT ct-9899 37 27 wi wi PROPN ct-9899 37 28 , , PUNCT ct-9899 37 29 us we PRON ct-9899 37 30 ) ) PUNCT ct-9899 37 31 into into ADP ct-9899 37 32 a a DET ct-9899 37 33 15 15 NUM ct-9899 37 34 - - PUNCT ct-9899 37 35 ml ml NOUN ct-9899 37 36 tube tube NOUN ct-9899 37 37 to to PART ct-9899 37 38 remove remove VERB ct-9899 37 39 small small ADJ ct-9899 37 40 tissue tissue NOUN ct-9899 37 41 fragments fragment NOUN ct-9899 37 42 and and CCONJ ct-9899 37 43 debris debris NOUN ct-9899 37 44 . . PUNCT ct-9899 38 1 a a DET ct-9899 38 2 complete complete ADJ ct-9899 38 3 semen semen NOUN ct-9899 38 4 evaluation evaluation NOUN ct-9899 38 5 was be AUX ct-9899 38 6 performed perform VERB ct-9899 38 7 as as SCONJ ct-9899 38 8 described describe VERB ct-9899 38 9 below below ADV ct-9899 38 10 , , PUNCT ct-9899 38 11 including include VERB ct-9899 38 12 evaluation evaluation NOUN ct-9899 38 13 of of ADP ct-9899 38 14 volume volume NOUN ct-9899 38 15 , , PUNCT ct-9899 38 16 sperm sperm NOUN ct-9899 38 17 concentration concentration NOUN ct-9899 38 18 , , PUNCT ct-9899 38 19 morphology morphology NOUN ct-9899 38 20 and and CCONJ ct-9899 38 21 motility motility NOUN ct-9899 38 22 . . PUNCT ct-9899 39 1 then then ADV ct-9899 39 2 , , PUNCT ct-9899 39 3 each each DET ct-9899 39 4 sample sample NOUN ct-9899 39 5 was be AUX ct-9899 39 6 divided divide VERB ct-9899 39 7 into into ADP ct-9899 39 8 two two NUM ct-9899 39 9 aliquots aliquot NOUN ct-9899 39 10 . . PUNCT ct-9899 40 1 both both DET ct-9899 40 2 aliquots aliquot NOUN ct-9899 40 3 were be AUX ct-9899 40 4 centrifuged centrifuge VERB ct-9899 40 5 at at ADP ct-9899 40 6 900 900 NUM ct-9899 40 7 x x SYM ct-9899 40 8 g g NOUN ct-9899 40 9 for for ADP ct-9899 40 10 10 10 NUM ct-9899 40 11 min min NOUN ct-9899 40 12 . . PUNCT ct-9899 41 1 this this DET ct-9899 41 2 centrifugation centrifugation NOUN ct-9899 41 3 force force NOUN ct-9899 41 4 has have VERB ct-9899 41 5 no no DET ct-9899 41 6 negative negative ADJ ct-9899 41 7 effects effect NOUN ct-9899 41 8 on on ADP ct-9899 41 9 sperm sperm NOUN ct-9899 41 10 motility motility NOUN ct-9899 41 11 or or CCONJ ct-9899 41 12 viability viability NOUN ct-9899 41 13 , , PUNCT ct-9899 41 14 and and CCONJ ct-9899 41 15 minimizes minimize VERB ct-9899 41 16 sperm sperm NOUN ct-9899 41 17 losses loss NOUN ct-9899 41 18 in in ADP ct-9899 41 19 the the DET ct-9899 41 20 supernatant.12 supernatant.12 NOUN ct-9899 41 21 after after ADP ct-9899 41 22 discarding discard VERB ct-9899 41 23 the the DET ct-9899 41 24 supernatant supernatant NOUN ct-9899 41 25 , , PUNCT ct-9899 41 26 one one NUM ct-9899 41 27 aliquot aliquot NOUN ct-9899 41 28 was be AUX ct-9899 41 29 resuspended resuspend VERB ct-9899 41 30 with with ADP ct-9899 41 31 the the DET ct-9899 41 32 liposome liposome NOUN ct-9899 41 33 - - PUNCT ct-9899 41 34 containing contain VERB ct-9899 41 35 semen semen NOUN ct-9899 41 36 extender extender NOUN ct-9899 41 37 optixcell optixcell PRON ct-9899 41 38 ( ( PUNCT ct-9899 41 39 imv imv PROPN ct-9899 41 40 technologies technology NOUN ct-9899 41 41 , , PUNCT ct-9899 41 42 maple maple PROPN ct-9899 41 43 grove grove PROPN ct-9899 41 44 , , PUNCT ct-9899 41 45 mn mn PROPN ct-9899 41 46 , , PUNCT ct-9899 41 47 usa usa PROPN ct-9899 41 48 ) ) PUNCT ct-9899 41 49 to to PART ct-9899 41 50 dilute dilute VERB ct-9899 41 51 sperm sperm NOUN ct-9899 41 52 to to ADP ct-9899 41 53 a a DET ct-9899 41 54 final final ADJ ct-9899 41 55 concentration concentration NOUN ct-9899 41 56 of of ADP ct-9899 41 57 25 25 NUM ct-9899 41 58 x x SYM ct-9899 41 59 106 106 NUM ct-9899 41 60 / / SYM ct-9899 41 61 ml ml NOUN ct-9899 41 62 . . PUNCT ct-9899 42 1 there there PRON ct-9899 42 2 are be VERB ct-9899 42 3 no no DET ct-9899 42 4 reports report NOUN ct-9899 42 5 in in ADP ct-9899 42 6 the the DET ct-9899 42 7 literature literature NOUN ct-9899 42 8 on on ADP ct-9899 42 9 the the DET ct-9899 42 10 ideal ideal ADJ ct-9899 42 11 sperm sperm NOUN ct-9899 42 12 concentration concentration NOUN ct-9899 42 13 for for ADP ct-9899 42 14 alpaca alpaca NOUN ct-9899 42 15 semen semen NOUN ct-9899 42 16 cryopreservation cryopreservation NOUN ct-9899 42 17 . . PUNCT ct-9899 43 1 the the DET ct-9899 43 2 concentration concentration NOUN ct-9899 43 3 used use VERB ct-9899 43 4 was be AUX ct-9899 43 5 selected select VERB ct-9899 43 6 to to PART ct-9899 43 7 enable enable VERB ct-9899 43 8 the the DET ct-9899 43 9 division division NOUN ct-9899 43 10 of of ADP ct-9899 43 11 the the DET ct-9899 43 12 samples sample NOUN ct-9899 43 13 into into ADP ct-9899 43 14 the the DET ct-9899 43 15 number number NOUN ct-9899 43 16 of of ADP ct-9899 43 17 aliquots aliquot NOUN ct-9899 43 18 needed need VERB ct-9899 43 19 . . PUNCT ct-9899 44 1 sperm sperm NOUN ct-9899 44 2 diluted dilute VERB ct-9899 44 3 in in ADP ct-9899 44 4 optixcell optixcell PRON ct-9899 44 5 were be AUX ct-9899 44 6 placed place VERB ct-9899 44 7 in in ADP ct-9899 44 8 the the DET ct-9899 44 9 refrigerator refrigerator NOUN ct-9899 44 10 at at ADP ct-9899 44 11 4 4 NUM ct-9899 44 12 ° ° ADP ct-9899 44 13 c c NOUN ct-9899 44 14 for for ADP ct-9899 44 15 5 5 NUM ct-9899 44 16 h h NOUN ct-9899 44 17 for for ADP ct-9899 44 18 equilibration equilibration NOUN ct-9899 44 19 . . PUNCT ct-9899 45 1 after after ADP ct-9899 45 2 equilibration equilibration NOUN ct-9899 45 3 , , PUNCT ct-9899 45 4 pre pre VERB ct-9899 45 5 - - VERB ct-9899 45 6 cooled cooled ADJ ct-9899 45 7 0.5 0.5 NUM ct-9899 45 8 ml ml NOUN ct-9899 45 9 french french ADJ ct-9899 45 10 straws straw NOUN ct-9899 45 11 were be AUX ct-9899 45 12 loaded load VERB ct-9899 45 13 . . PUNCT ct-9899 46 1 the the DET ct-9899 46 2 other other ADJ ct-9899 46 3 centrifuged centrifuged ADJ ct-9899 46 4 aliquot aliquot NOUN ct-9899 46 5 was be AUX ct-9899 46 6 diluted dilute VERB ct-9899 46 7 with with ADP ct-9899 46 8 the the DET ct-9899 46 9 dmfa dmfa NOUN ct-9899 46 10 - - PUNCT ct-9899 46 11 containing contain VERB ct-9899 46 12 semen semen NOUN ct-9899 46 13 extender extender NOUN ct-9899 46 14 botucrio botucrio NOUN ct-9899 46 15 ( ( PUNCT ct-9899 46 16 botupharma botupharma PROPN ct-9899 46 17 usa usa PROPN ct-9899 46 18 , , PUNCT ct-9899 46 19 phoenix phoenix PROPN ct-9899 46 20 , , PUNCT ct-9899 46 21 az az PROPN ct-9899 46 22 , , PUNCT ct-9899 46 23 usa usa PROPN ct-9899 46 24 ) ) PUNCT ct-9899 46 25 until until SCONJ ct-9899 46 26 a a DET ct-9899 46 27 final final ADJ ct-9899 46 28 sperm sperm NOUN ct-9899 46 29 concentration concentration NOUN ct-9899 46 30 of of ADP ct-9899 46 31 25 25 NUM ct-9899 46 32 x x SYM ct-9899 46 33 106 106 NUM ct-9899 46 34 / / SYM ct-9899 46 35 ml ml NOUN ct-9899 46 36 was be AUX ct-9899 46 37 reached reach VERB ct-9899 46 38 . . PUNCT ct-9899 47 1 sperm sperm NOUN ct-9899 47 2 diluted dilute VERB ct-9899 47 3 in in ADP ct-9899 47 4 botucrio botucrio NOUN ct-9899 47 5 were be AUX ct-9899 47 6 loaded load VERB ct-9899 47 7 into into ADP ct-9899 47 8 0.5 0.5 NUM ct-9899 47 9 - - PUNCT ct-9899 47 10 ml ml ADP ct-9899 47 11 french french ADJ ct-9899 47 12 straws straw NOUN ct-9899 47 13 at at ADP ct-9899 47 14 room room NOUN ct-9899 47 15 temperature temperature NOUN ct-9899 47 16 , , PUNCT ct-9899 47 17 and and CCONJ ct-9899 47 18 equilibrated equilibrate VERB ct-9899 47 19 at at ADP ct-9899 47 20 4 4 NUM ct-9899 47 21 ° ° ADP ct-9899 47 22 c c NOUN ct-9899 47 23 for for ADP ct-9899 47 24 15 15 NUM ct-9899 47 25 min min NOUN ct-9899 47 26 . . PROPN ct-9899 47 27 next next ADV ct-9899 47 28 , , PUNCT ct-9899 47 29 all all DET ct-9899 47 30 straws straw NOUN ct-9899 47 31 were be AUX ct-9899 47 32 placed place VERB ct-9899 47 33 horizontally horizontally ADV ct-9899 47 34 on on ADP ct-9899 47 35 a a DET ct-9899 47 36 rack rack NOUN ct-9899 47 37 , , PUNCT ct-9899 47 38 3 3 NUM ct-9899 47 39 cm cm NOUN ct-9899 47 40 above above ADP ct-9899 47 41 liquid liquid ADJ ct-9899 47 42 nitrogen nitrogen NOUN ct-9899 47 43 for for ADP ct-9899 47 44 15 15 NUM ct-9899 47 45 min min NOUN ct-9899 47 46 . . PUNCT ct-9899 48 1 the the DET ct-9899 48 2 straws straw NOUN ct-9899 48 3 were be AUX ct-9899 48 4 plunged plunge VERB ct-9899 48 5 in in ADP ct-9899 48 6 liquid liquid ADJ ct-9899 48 7 nitrogen nitrogen NOUN ct-9899 48 8 , , PUNCT ct-9899 48 9 loaded load VERB ct-9899 48 10 into into ADP ct-9899 48 11 10 10 NUM ct-9899 48 12 - - PUNCT ct-9899 48 13 mm mm NOUN ct-9899 48 14 goblets goblet NOUN ct-9899 48 15 , , PUNCT ct-9899 48 16 and and CCONJ ct-9899 48 17 transferred transfer VERB ct-9899 48 18 to to ADP ct-9899 48 19 the the DET ct-9899 48 20 storage storage NOUN ct-9899 48 21 tank tank NOUN ct-9899 48 22 . . PUNCT ct-9899 49 1 all all DET ct-9899 49 2 straws straw NOUN ct-9899 49 3 were be AUX ct-9899 49 4 stored store VERB ct-9899 49 5 at at ADP ct-9899 49 6 196c 196c NUM ct-9899 49 7 until until ADP ct-9899 49 8 evaluation evaluation NOUN ct-9899 49 9 . . PUNCT ct-9899 50 1 the the DET ct-9899 50 2 cryopreservation cryopreservation NOUN ct-9899 50 3 protocols protocol NOUN ct-9899 50 4 used use VERB ct-9899 50 5 were be AUX ct-9899 50 6 based base VERB ct-9899 50 7 on on ADP ct-9899 50 8 instructions instruction NOUN ct-9899 50 9 from from ADP ct-9899 50 10 the the DET ct-9899 50 11 manufacturer manufacturer NOUN ct-9899 50 12 of of ADP ct-9899 50 13 each each DET ct-9899 50 14 semen semen NOUN ct-9899 50 15 extender extender NOUN ct-9899 50 16 . . PUNCT ct-9899 51 1 the the DET ct-9899 51 2 other other ADJ ct-9899 51 3 epididymis epididymis NOUN ct-9899 51 4 from from ADP ct-9899 51 5 each each DET ct-9899 51 6 male male NOUN ct-9899 51 7 was be AUX ct-9899 51 8 maintained maintain VERB ct-9899 51 9 in in ADP ct-9899 51 10 the the DET ct-9899 51 11 plastic plastic ADJ ct-9899 51 12 420clinical 420clinical PROPN ct-9899 51 13 theriogenology theriogenology NOUN ct-9899 51 14 • • NOUN ct-9899 51 15 volume volume NOUN ct-9899 51 16 10 10 NUM ct-9899 51 17 , , PUNCT ct-9899 51 18 number number NOUN ct-9899 51 19 4 4 NUM ct-9899 51 20 • • NOUN ct-9899 51 21 december december PROPN ct-9899 51 22 2018 2018 NUM ct-9899 51 23 bag bag NOUN ct-9899 51 24 at at ADP ct-9899 51 25 4 4 NUM ct-9899 51 26 ° ° ADP ct-9899 51 27 c c NOUN ct-9899 51 28 for for ADP ct-9899 51 29 24 24 NUM ct-9899 51 30 h. h. PROPN ct-9899 51 31 after after ADP ct-9899 51 32 24 24 NUM ct-9899 51 33 h h NOUN ct-9899 51 34 of of ADP ct-9899 51 35 cold cold ADJ ct-9899 51 36 storage storage NOUN ct-9899 51 37 , , PUNCT ct-9899 51 38 the the DET ct-9899 51 39 cauda cauda NOUN ct-9899 51 40 epididymides epididymis NOUN ct-9899 51 41 were be AUX ct-9899 51 42 processed process VERB ct-9899 51 43 as as SCONJ ct-9899 51 44 described describe VERB ct-9899 51 45 for for ADP ct-9899 51 46 the the DET ct-9899 51 47 contralateral contralateral ADJ ct-9899 51 48 testis testis NOUN ct-9899 51 49 . . PUNCT ct-9899 52 1 each each DET ct-9899 52 2 sample sample NOUN ct-9899 52 3 was be AUX ct-9899 52 4 also also ADV ct-9899 52 5 divided divide VERB ct-9899 52 6 into into ADP ct-9899 52 7 two two NUM ct-9899 52 8 aliquots aliquot NOUN ct-9899 52 9 , , PUNCT ct-9899 52 10 diluted dilute VERB ct-9899 52 11 with with ADP ct-9899 52 12 optixcell optixcell NUM ct-9899 52 13 or or CCONJ ct-9899 52 14 botucrio botucrio NOUN ct-9899 52 15 , , PUNCT ct-9899 52 16 and and CCONJ ct-9899 52 17 cryopreserved cryopreserve VERB ct-9899 52 18 following follow VERB ct-9899 52 19 the the DET ct-9899 52 20 same same ADJ ct-9899 52 21 protocols protocol NOUN ct-9899 52 22 as as SCONJ ct-9899 52 23 described describe VERB ct-9899 52 24 above above ADV ct-9899 52 25 . . PUNCT ct-9899 53 1 two two NUM ct-9899 53 2 weeks week NOUN ct-9899 53 3 after after ADP ct-9899 53 4 freezing freeze VERB ct-9899 53 5 , , PUNCT ct-9899 53 6 one one NUM ct-9899 53 7 straw straw NOUN ct-9899 53 8 from from ADP ct-9899 53 9 each each DET ct-9899 53 10 aliquot aliquot NOUN ct-9899 53 11 was be AUX ct-9899 53 12 thawed thaw VERB ct-9899 53 13 in in ADP ct-9899 53 14 a a DET ct-9899 53 15 water water NOUN ct-9899 53 16 bath bath NOUN ct-9899 53 17 at at ADP ct-9899 53 18 38c 38c NUM ct-9899 53 19 for for ADP ct-9899 53 20 30 30 NUM ct-9899 53 21 s s NOUN ct-9899 53 22 , , PUNCT ct-9899 53 23 and and CCONJ ct-9899 53 24 sperm sperm NOUN ct-9899 53 25 motility motility NOUN ct-9899 53 26 and and CCONJ ct-9899 53 27 membrane membrane NOUN ct-9899 53 28 integrity integrity NOUN ct-9899 53 29 were be AUX ct-9899 53 30 evaluated evaluate VERB ct-9899 53 31 . . PUNCT ct-9899 54 1 another another DET ct-9899 54 2 straw straw NOUN ct-9899 54 3 was be AUX ct-9899 54 4 thawed thaw VERB ct-9899 54 5 for for ADP ct-9899 54 6 evaluation evaluation NOUN ct-9899 54 7 of of ADP ct-9899 54 8 dna dna PROPN ct-9899 54 9 integrity integrity NOUN ct-9899 54 10 . . PUNCT ct-9899 55 1 semen semen NOUN ct-9899 55 2 evaluation evaluation NOUN ct-9899 55 3 immediately immediately ADV ct-9899 55 4 after after ADP ct-9899 55 5 recovery recovery NOUN ct-9899 55 6 of of ADP ct-9899 55 7 sperm sperm NOUN ct-9899 55 8 , , PUNCT ct-9899 55 9 sample sample NOUN ct-9899 55 10 volume volume NOUN ct-9899 55 11 was be AUX ct-9899 55 12 determined determine VERB ct-9899 55 13 using use VERB ct-9899 55 14 a a DET ct-9899 55 15 graduated graduate VERB ct-9899 55 16 15 15 NUM ct-9899 55 17 - - PUNCT ct-9899 55 18 ml ml ADV ct-9899 55 19 centrifuge centrifuge NOUN ct-9899 55 20 tube tube NOUN ct-9899 55 21 . . PUNCT ct-9899 56 1 sperm sperm NOUN ct-9899 56 2 concentration concentration NOUN ct-9899 56 3 and and CCONJ ct-9899 56 4 motility motility NOUN ct-9899 56 5 were be AUX ct-9899 56 6 evaluated evaluate VERB ct-9899 56 7 with with ADP ct-9899 56 8 a a DET ct-9899 56 9 computer computer NOUN ct-9899 56 10 assisted assist VERB ct-9899 56 11 semen semen NOUN ct-9899 56 12 analyzer analyzer NOUN ct-9899 56 13 ( ( PUNCT ct-9899 56 14 casa casa PROPN ct-9899 56 15 ; ; PUNCT ct-9899 56 16 spermvision spermvision PROPN ct-9899 56 17 professional professional NOUN ct-9899 56 18 , , PUNCT ct-9899 56 19 minitube minitube NOUN ct-9899 56 20 of of ADP ct-9899 56 21 america america PROPN ct-9899 56 22 , , PUNCT ct-9899 56 23 verona verona PROPN ct-9899 56 24 , , PUNCT ct-9899 56 25 wi wi PROPN ct-9899 56 26 , , PUNCT ct-9899 56 27 usa usa PROPN ct-9899 56 28 ) ) PUNCT ct-9899 56 29 . . PUNCT ct-9899 57 1 the the DET ct-9899 57 2 settings setting NOUN ct-9899 57 3 of of ADP ct-9899 57 4 the the DET ct-9899 57 5 instrument instrument NOUN ct-9899 57 6 were be AUX ct-9899 57 7 : : PUNCT ct-9899 57 8 field field NOUN ct-9899 57 9 depth depth NOUN ct-9899 57 10 of of ADP ct-9899 57 11 view view NOUN ct-9899 57 12 20 20 NUM ct-9899 57 13 m m PROPN ct-9899 57 14 , , PUNCT ct-9899 57 15 pixel pixel VERB ct-9899 57 16 to to ADP ct-9899 57 17 m m PROPN ct-9899 57 18 ratio ratio NOUN ct-9899 57 19 130 130 NUM ct-9899 57 20 to to PART ct-9899 57 21 100 100 NUM ct-9899 57 22 , , PUNCT ct-9899 57 23 cell cell NOUN ct-9899 57 24 area area NOUN ct-9899 57 25 18 18 NUM ct-9899 57 26 to to PART ct-9899 57 27 80 80 NUM ct-9899 57 28 m m PROPN ct-9899 57 29 , , PUNCT ct-9899 57 30 frames frame NOUN ct-9899 57 31 acquired acquire VERB ct-9899 57 32 30 30 NUM ct-9899 57 33 , , PUNCT ct-9899 57 34 frame frame NOUN ct-9899 57 35 rate rate NOUN ct-9899 57 36 60 60 NUM ct-9899 57 37 hz hz VERB ct-9899 57 38 , , PUNCT ct-9899 57 39 aoc aoc NOUN ct-9899 57 40 cut cut VERB ct-9899 57 41 off off ADP ct-9899 57 42 static static ADJ ct-9899 57 43 cells cell NOUN ct-9899 57 44 5 5 NUM ct-9899 57 45 and and CCONJ ct-9899 57 46 dsl dsl AUX ct-9899 57 47 cut cut VERB ct-9899 57 48 off off ADP ct-9899 57 49 4.5 4.5 NUM ct-9899 57 50 µm µm NOUN ct-9899 57 51 / / SYM ct-9899 57 52 s s X ct-9899 57 53 ( ( PUNCT ct-9899 57 54 progressive progressive ADJ ct-9899 57 55 motility motility NOUN ct-9899 57 56 ) ) PUNCT ct-9899 57 57 . . PUNCT ct-9899 58 1 semen seman NOUN ct-9899 58 2 was be AUX ct-9899 58 3 placed place VERB ct-9899 58 4 in in ADP ct-9899 58 5 a a DET ct-9899 58 6 20-µl 20-µl PROPN ct-9899 58 7 sperm sperm NOUN ct-9899 58 8 analysis analysis NOUN ct-9899 58 9 chamber chamber NOUN ct-9899 58 10 over over ADP ct-9899 58 11 the the DET ct-9899 58 12 heated heated ADJ ct-9899 58 13 specimen speciman NOUN ct-9899 58 14 stage stage NOUN ct-9899 58 15 at at ADP ct-9899 58 16 38c 38c NUM ct-9899 58 17 . . PUNCT ct-9899 59 1 mean mean VERB ct-9899 59 2 percentage percentage NOUN ct-9899 59 3 of of ADP ct-9899 59 4 total total ADJ ct-9899 59 5 ( ( PUNCT ct-9899 59 6 tmot tmot NOUN ct-9899 59 7 ) ) PUNCT ct-9899 59 8 and and CCONJ ct-9899 59 9 progressively progressively ADV ct-9899 59 10 ( ( PUNCT ct-9899 59 11 pmot pmot NOUN ct-9899 59 12 ) ) PUNCT ct-9899 59 13 motile motile ADJ ct-9899 59 14 sperm sperm NOUN ct-9899 59 15 was be AUX ct-9899 59 16 assessed assess VERB ct-9899 59 17 from from ADP ct-9899 59 18 all all DET ct-9899 59 19 cells cell NOUN ct-9899 59 20 present present ADJ ct-9899 59 21 in in ADP ct-9899 59 22 seven seven NUM ct-9899 59 23 fields field NOUN ct-9899 59 24 with with ADP ct-9899 59 25 a a DET ct-9899 59 26 x x SYM ct-9899 59 27 20 20 NUM ct-9899 59 28 phase phase NOUN ct-9899 59 29 - - PUNCT ct-9899 59 30 contrast contrast NOUN ct-9899 59 31 objective objective NOUN ct-9899 59 32 . . PUNCT ct-9899 60 1 phase phase NOUN ct-9899 60 2 contrast contrast NOUN ct-9899 60 3 microscopy microscopy NOUN ct-9899 60 4 was be AUX ct-9899 60 5 used use VERB ct-9899 60 6 for for ADP ct-9899 60 7 assessment assessment NOUN ct-9899 60 8 of of ADP ct-9899 60 9 sperm sperm NOUN ct-9899 60 10 morphology morphology NOUN ct-9899 60 11 . . PUNCT ct-9899 61 1 sperm sperm NOUN ct-9899 61 2 were be AUX ct-9899 61 3 diluted dilute VERB ct-9899 61 4 1:10 1:10 NUM ct-9899 61 5 ( ( PUNCT ct-9899 61 6 v v NOUN ct-9899 61 7 : : PUNCT ct-9899 61 8 v v NOUN ct-9899 61 9 ) ) PUNCT ct-9899 61 10 with with ADP ct-9899 61 11 formalin formalin NOUN ct-9899 61 12 buffered buffer VERB ct-9899 61 13 solution solution NOUN ct-9899 61 14 , , PUNCT ct-9899 61 15 and and CCONJ ct-9899 61 16 a a DET ct-9899 61 17 5l 5l NUM ct-9899 61 18 drop drop NOUN ct-9899 61 19 was be AUX ct-9899 61 20 placed place VERB ct-9899 61 21 on on ADP ct-9899 61 22 a a DET ct-9899 61 23 microscope microscope NOUN ct-9899 61 24 slide slide NOUN ct-9899 61 25 . . PUNCT ct-9899 62 1 evaluation evaluation NOUN ct-9899 62 2 was be AUX ct-9899 62 3 done do VERB ct-9899 62 4 at at ADP ct-9899 62 5 100x 100x NUM ct-9899 62 6 magnification magnification NOUN ct-9899 62 7 under under ADP ct-9899 62 8 oil oil NOUN ct-9899 62 9 immersion immersion NOUN ct-9899 62 10 , , PUNCT ct-9899 62 11 and and CCONJ ct-9899 62 12 100 100 NUM ct-9899 62 13 sperm sperm NOUN ct-9899 62 14 were be AUX ct-9899 62 15 classified classify VERB ct-9899 62 16 based base VERB ct-9899 62 17 on on ADP ct-9899 62 18 their their PRON ct-9899 62 19 morphological morphological ADJ ct-9899 62 20 characteristics characteristic NOUN ct-9899 62 21 . . PUNCT ct-9899 63 1 two two NUM ct-9899 63 2 weeks week NOUN ct-9899 63 3 after after ADP ct-9899 63 4 freezing freeze VERB ct-9899 63 5 , , PUNCT ct-9899 63 6 one one NUM ct-9899 63 7 straw straw NOUN ct-9899 63 8 from from ADP ct-9899 63 9 each each DET ct-9899 63 10 aliquot aliquot NOUN ct-9899 63 11 was be AUX ct-9899 63 12 thawed thaw VERB ct-9899 63 13 in in ADP ct-9899 63 14 a a DET ct-9899 63 15 water water NOUN ct-9899 63 16 bath bath NOUN ct-9899 63 17 at at ADP ct-9899 63 18 38c 38c NUM ct-9899 63 19 for for ADP ct-9899 63 20 30 30 NUM ct-9899 63 21 s s NOUN ct-9899 63 22 , , PUNCT ct-9899 63 23 and and CCONJ ct-9899 63 24 sperm sperm NOUN ct-9899 63 25 motility motility NOUN ct-9899 63 26 and and CCONJ ct-9899 63 27 membrane membrane NOUN ct-9899 63 28 integrity integrity NOUN ct-9899 63 29 were be AUX ct-9899 63 30 evaluated evaluate VERB ct-9899 63 31 using use VERB ct-9899 63 32 casa casa PROPN ct-9899 63 33 . . PUNCT ct-9899 64 1 in in ADP ct-9899 64 2 addition addition NOUN ct-9899 64 3 to to ADP ct-9899 64 4 tmot tmot NOUN ct-9899 64 5 and and CCONJ ct-9899 64 6 pmot pmot NOUN ct-9899 64 7 , , PUNCT ct-9899 64 8 other other ADJ ct-9899 64 9 motion motion NOUN ct-9899 64 10 parameters parameter NOUN ct-9899 64 11 analyzed analyze VERB ct-9899 64 12 post post ADJ ct-9899 64 13 - - ADJ ct-9899 64 14 thaw thaw NOUN ct-9899 64 15 were be AUX ct-9899 64 16 : : PUNCT ct-9899 64 17 average average ADJ ct-9899 64 18 path path NOUN ct-9899 64 19 velocity velocity NOUN ct-9899 64 20 ( ( PUNCT ct-9899 64 21 vap vap NOUN ct-9899 64 22 ) ) PUNCT ct-9899 64 23 , , PUNCT ct-9899 64 24 straight straight ADJ ct-9899 64 25 - - PUNCT ct-9899 64 26 line line NOUN ct-9899 64 27 velocity velocity NOUN ct-9899 64 28 ( ( PUNCT ct-9899 64 29 vsl vsl NOUN ct-9899 64 30 ) ) PUNCT ct-9899 64 31 , , PUNCT ct-9899 64 32 curvilinear curvilinear ADJ ct-9899 64 33 velocity velocity NOUN ct-9899 64 34 ( ( PUNCT ct-9899 64 35 vcl vcl PROPN ct-9899 64 36 ) ) PUNCT ct-9899 64 37 , , PUNCT ct-9899 64 38 amplitude amplitude NOUN ct-9899 64 39 of of ADP ct-9899 64 40 lateral lateral ADJ ct-9899 64 41 head head NOUN ct-9899 64 42 displacement displacement NOUN ct-9899 64 43 ( ( PUNCT ct-9899 64 44 alh alh PROPN ct-9899 64 45 ) ) PUNCT ct-9899 64 46 , , PUNCT ct-9899 64 47 beat beat VERB ct-9899 64 48 cross cross NOUN ct-9899 64 49 frequency frequency NOUN ct-9899 64 50 ( ( PUNCT ct-9899 64 51 bcf bcf NOUN ct-9899 64 52 ) ) PUNCT ct-9899 64 53 , , PUNCT ct-9899 64 54 straightness straightness NOUN ct-9899 64 55 ( ( PUNCT ct-9899 64 56 str str NOUN ct-9899 64 57 ) ) PUNCT ct-9899 64 58 , , PUNCT ct-9899 64 59 linearity linearity NOUN ct-9899 64 60 ( ( PUNCT ct-9899 64 61 lin lin PROPN ct-9899 64 62 ) ) PUNCT ct-9899 64 63 , , PUNCT ct-9899 64 64 wobble wobble ADJ ct-9899 64 65 ( ( PUNCT ct-9899 64 66 wob wob NOUN ct-9899 64 67 ) ) PUNCT ct-9899 64 68 , , PUNCT ct-9899 64 69 curvilinear curvilinear ADJ ct-9899 64 70 distance distance NOUN ct-9899 64 71 ( ( PUNCT ct-9899 64 72 dcl dcl NOUN ct-9899 64 73 ) ) PUNCT ct-9899 64 74 , , PUNCT ct-9899 64 75 straight straight ADJ ct-9899 64 76 line line NOUN ct-9899 64 77 distance distance NOUN ct-9899 64 78 ( ( PUNCT ct-9899 64 79 dsl dsl ADJ ct-9899 64 80 ) ) PUNCT ct-9899 64 81 , , PUNCT ct-9899 64 82 and and CCONJ ct-9899 64 83 distance distance NOUN ct-9899 64 84 of of ADP ct-9899 64 85 average average ADJ ct-9899 64 86 path path NOUN ct-9899 64 87 ( ( PUNCT ct-9899 64 88 dap dap PROPN ct-9899 64 89 ) ) PUNCT ct-9899 64 90 . . PUNCT ct-9899 65 1 membrane membrane NOUN ct-9899 65 2 integrity integrity NOUN ct-9899 65 3 was be AUX ct-9899 65 4 evaluated evaluate VERB ct-9899 65 5 using use VERB ct-9899 65 6 fluorescence fluorescence NOUN ct-9899 65 7 microscopy microscopy NOUN ct-9899 65 8 under under ADP ct-9899 65 9 a a DET ct-9899 65 10 40x 40x NOUN ct-9899 65 11 objective objective NOUN ct-9899 65 12 . . PUNCT ct-9899 66 1 sperm sperm NOUN ct-9899 66 2 were be AUX ct-9899 66 3 incubated incubate VERB ct-9899 66 4 for for ADP ct-9899 66 5 10 10 NUM ct-9899 66 6 min min NOUN ct-9899 66 7 at at ADP ct-9899 66 8 38c 38c NUM ct-9899 66 9 with with ADP ct-9899 66 10 the the DET ct-9899 66 11 vital vital ADJ ct-9899 66 12 stains stain NOUN ct-9899 66 13 sybr14 sybr14 NOUN ct-9899 66 14 and and CCONJ ct-9899 66 15 propidium propidium NOUN ct-9899 66 16 iodide iodide NOUN ct-9899 66 17 ( ( PUNCT ct-9899 66 18 live live ADJ ct-9899 66 19 / / SYM ct-9899 66 20 dead dead ADJ ct-9899 66 21 kit kit NOUN ct-9899 66 22 , , PUNCT ct-9899 66 23 minitube minitube NOUN ct-9899 66 24 of of ADP ct-9899 66 25 america america PROPN ct-9899 66 26 ) ) PUNCT ct-9899 66 27 following follow VERB ct-9899 66 28 instructions instruction NOUN ct-9899 66 29 from from ADP ct-9899 66 30 the the DET ct-9899 66 31 manufacturer manufacturer NOUN ct-9899 66 32 . . PUNCT ct-9899 67 1 all all DET ct-9899 67 2 sperm sperm NOUN ct-9899 67 3 presented present VERB ct-9899 67 4 in in ADP ct-9899 67 5 10 10 NUM ct-9899 67 6 fields field NOUN ct-9899 67 7 were be AUX ct-9899 67 8 classified classify VERB ct-9899 67 9 as as ADP ct-9899 67 10 membrane membrane NOUN ct-9899 67 11 - - PUNCT ct-9899 67 12 intact intact ADJ ct-9899 67 13 ( ( PUNCT ct-9899 67 14 green green ADJ ct-9899 67 15 fluorescence fluorescence NOUN ct-9899 67 16 ) ) PUNCT ct-9899 67 17 or or CCONJ ct-9899 67 18 membrane membrane NOUN ct-9899 67 19 - - PUNCT ct-9899 67 20 damaged damage VERB ct-9899 67 21 ( ( PUNCT ct-9899 67 22 red red ADJ ct-9899 67 23 fluorescence fluorescence NOUN ct-9899 67 24 ) ) PUNCT ct-9899 67 25 by by ADP ct-9899 67 26 a a DET ct-9899 67 27 built build VERB ct-9899 67 28 - - PUNCT ct-9899 67 29 in in ADP ct-9899 67 30 software software NOUN ct-9899 67 31 within within ADP ct-9899 67 32 the the DET ct-9899 67 33 casa casa PROPN ct-9899 67 34 system system NOUN ct-9899 67 35 ( ( PUNCT ct-9899 67 36 spermvision spermvision NOUN ct-9899 67 37 professionaltm professionaltm PROPN ct-9899 67 38 , , PUNCT ct-9899 67 39 minitube minitube NOUN ct-9899 67 40 of of ADP ct-9899 67 41 america america PROPN ct-9899 67 42 ) ) PUNCT ct-9899 67 43 , , PUNCT ct-9899 67 44 and and CCONJ ct-9899 67 45 mean mean VERB ct-9899 67 46 percentages percentage NOUN ct-9899 67 47 were be AUX ct-9899 67 48 reported report VERB ct-9899 67 49 . . PUNCT ct-9899 68 1 sperm sperm NOUN ct-9899 68 2 chromatin chromatin NOUN ct-9899 68 3 structure structure NOUN ct-9899 68 4 was be AUX ct-9899 68 5 evaluated evaluate VERB ct-9899 68 6 on on ADP ct-9899 68 7 frozen freeze VERB ct-9899 68 8 - - PUNCT ct-9899 68 9 thawed thaw VERB ct-9899 68 10 semen semen NOUN ct-9899 68 11 using use VERB ct-9899 68 12 acridine acridine PROPN ct-9899 68 13 orange orange PROPN ct-9899 68 14 and and CCONJ ct-9899 68 15 flow flow NOUN ct-9899 68 16 cytometry cytometry NOUN ct-9899 68 17 ( ( PUNCT ct-9899 68 18 accuri accuri ADP ct-9899 68 19 c6 c6 PROPN ct-9899 68 20 plus plus CCONJ ct-9899 68 21 , , PUNCT ct-9899 68 22 bd bd PROPN ct-9899 68 23 biosciences bioscience NOUN ct-9899 68 24 , , PUNCT ct-9899 68 25 san san PROPN ct-9899 68 26 jose jose PROPN ct-9899 68 27 ca ca PROPN ct-9899 68 28 , , PUNCT ct-9899 68 29 usa usa PROPN ct-9899 68 30 ) ) PUNCT ct-9899 68 31 . . PUNCT ct-9899 69 1 frozen frozen ADJ ct-9899 69 2 - - PUNCT ct-9899 69 3 thawed thaw VERB ct-9899 69 4 sperm sperm NOUN ct-9899 69 5 were be AUX ct-9899 69 6 washed wash VERB ct-9899 69 7 in in ADP ct-9899 69 8 phosphate phosphate ADJ ct-9899 69 9 buffer buffer NOUN ct-9899 69 10 solution solution NOUN ct-9899 69 11 by by ADP ct-9899 69 12 centrifugation centrifugation NOUN ct-9899 69 13 at at ADP ct-9899 69 14 600 600 NUM ct-9899 69 15 x x SYM ct-9899 69 16 g g NOUN ct-9899 69 17 for for ADP ct-9899 69 18 5 5 NUM ct-9899 69 19 min min NOUN ct-9899 69 20 . . NOUN ct-9899 69 21 after after ADP ct-9899 69 22 removing remove VERB ct-9899 69 23 the the DET ct-9899 69 24 supernatant supernatant NOUN ct-9899 69 25 , , PUNCT ct-9899 69 26 the the DET ct-9899 69 27 pellet pellet NOUN ct-9899 69 28 was be AUX ct-9899 69 29 re re VERB ct-9899 69 30 - - VERB ct-9899 69 31 suspended suspend VERB ct-9899 69 32 to to ADP ct-9899 69 33 0.5 0.5 NUM ct-9899 69 34 ml ml NOUN ct-9899 69 35 in in ADP ct-9899 69 36 tne tne NOUN ct-9899 69 37 buffer buffer NOUN ct-9899 69 38 ( ( PUNCT ct-9899 69 39 0.186 0.186 NUM ct-9899 69 40 g g PROPN ct-9899 69 41 disodium disodium NOUN ct-9899 69 42 etda etda NOUN ct-9899 69 43 , , PUNCT ct-9899 69 44 0.79 0.79 NUM ct-9899 69 45 g g PROPN ct-9899 69 46 tris tris PROPN ct-9899 69 47 - - PUNCT ct-9899 69 48 hcl hcl NOUN ct-9899 69 49 , , PUNCT ct-9899 69 50 4.380 4.380 NUM ct-9899 69 51 g g PROPN ct-9899 69 52 nacl nacl NOUN ct-9899 69 53 in in ADP ct-9899 69 54 500 500 NUM ct-9899 69 55 ml ml ADV ct-9899 69 56 deionized deionize VERB ct-9899 69 57 water water NOUN ct-9899 69 58 , , PUNCT ct-9899 69 59 ph ph NOUN ct-9899 69 60 7.4 7.4 NUM ct-9899 69 61 , , PUNCT ct-9899 69 62 4 4 NUM ct-9899 69 63 ° ° NOUN ct-9899 69 64 c c NOUN ct-9899 69 65 ) ) PUNCT ct-9899 69 66 . . PUNCT ct-9899 70 1 then then ADV ct-9899 70 2 , , PUNCT ct-9899 70 3 15 15 NUM ct-9899 70 4 l l NOUN ct-9899 70 5 of of ADP ct-9899 70 6 the the DET ct-9899 70 7 resuspended resuspend VERB ct-9899 70 8 sample sample NOUN ct-9899 70 9 was be AUX ct-9899 70 10 diluted dilute VERB ct-9899 70 11 with with ADP ct-9899 70 12 185 185 NUM ct-9899 70 13 l l PROPN ct-9899 70 14 of of ADP ct-9899 70 15 tne tne NOUN ct-9899 70 16 buffer buffer NOUN ct-9899 70 17 . . PUNCT ct-9899 71 1 this this DET ct-9899 71 2 dilution dilution NOUN ct-9899 71 3 provided provide VERB ct-9899 71 4 a a DET ct-9899 71 5 final final ADJ ct-9899 71 6 sperm sperm NOUN ct-9899 71 7 concentration concentration NOUN ct-9899 71 8 of of ADP ct-9899 71 9 2 2 NUM ct-9899 71 10 x x SYM ct-9899 71 11 106 106 NUM ct-9899 71 12 / / SYM ct-9899 71 13 ml ml NOUN ct-9899 71 14 . . PUNCT ct-9899 72 1 semen semen PROPN ct-9899 72 2 was be AUX ct-9899 72 3 incubated incubate VERB ct-9899 72 4 for for ADP ct-9899 72 5 30 30 NUM ct-9899 72 6 s s NOUN ct-9899 72 7 after after ADP ct-9899 72 8 addition addition NOUN ct-9899 72 9 of of ADP ct-9899 72 10 400 400 NUM ct-9899 72 11 l l PROPN ct-9899 72 12 of of ADP ct-9899 72 13 acid acid NOUN ct-9899 72 14 - - PUNCT ct-9899 72 15 detergent detergent NOUN ct-9899 72 16 solution solution NOUN ct-9899 72 17 ( ( PUNCT ct-9899 72 18 2.19 2.19 NUM ct-9899 72 19 g g NOUN ct-9899 72 20 nacl nacl NOUN ct-9899 72 21 , , PUNCT ct-9899 72 22 1 1 NUM ct-9899 72 23 ml ml NUM ct-9899 72 24 2n 2n NUM ct-9899 72 25 hcl hcl PROPN ct-9899 72 26 , , PUNCT ct-9899 72 27 0.25 0.25 NUM ct-9899 72 28 ml ml PROPN ct-9899 72 29 triton triton PROPN ct-9899 72 30 x x PROPN ct-9899 72 31 , , PUNCT ct-9899 72 32 q.s q.s PROPN ct-9899 72 33 . . PROPN ct-9899 72 34 250 250 NUM ct-9899 72 35 ml ml NOUN ct-9899 72 36 deionized deionize VERB ct-9899 72 37 water water NOUN ct-9899 72 38 , , PUNCT ct-9899 72 39 ph ph NOUN ct-9899 72 40 1.2 1.2 NUM ct-9899 72 41 , , PUNCT ct-9899 72 42 4 4 NUM ct-9899 72 43 ° ° NOUN ct-9899 72 44 c c NOUN ct-9899 72 45 ) ) PUNCT ct-9899 72 46 . . PUNCT ct-9899 73 1 finally finally ADV ct-9899 73 2 , , PUNCT ct-9899 73 3 1.2 1.2 NUM ct-9899 73 4 ml ml NOUN ct-9899 73 5 of of ADP ct-9899 73 6 a a DET ct-9899 73 7 2 2 NUM ct-9899 73 8 g g NOUN ct-9899 73 9 / / SYM ct-9899 73 10 ml ml X ct-9899 73 11 working working NOUN ct-9899 73 12 solution solution NOUN ct-9899 73 13 of of ADP ct-9899 73 14 acridine acridine PROPN ct-9899 73 15 orange orange PROPN ct-9899 73 16 ( ( PUNCT ct-9899 73 17 invitrogen invitrogen PROPN ct-9899 73 18 , , PUNCT ct-9899 73 19 waltham waltham PROPN ct-9899 73 20 , , PUNCT ct-9899 73 21 ma ma PROPN ct-9899 73 22 , , PUNCT ct-9899 73 23 usa usa PROPN ct-9899 73 24 , , PUNCT ct-9899 73 25 cat cat NOUN ct-9899 73 26 . . PUNCT ct-9899 74 1 no no INTJ ct-9899 74 2 . . PUNCT ct-9899 75 1 a3568 a3568 NOUN ct-9899 75 2 ) ) PUNCT ct-9899 76 1 in in ADP ct-9899 76 2 buffer buffer NOUN ct-9899 76 3 ( ( PUNCT ct-9899 76 4 3.8869 3.8869 NUM ct-9899 76 5 g g NOUN ct-9899 76 6 citric citric ADJ ct-9899 76 7 acid acid NOUN ct-9899 76 8 monohydrate monohydrate NOUN ct-9899 76 9 , , PUNCT ct-9899 76 10 8.9429 8.9429 NUM ct-9899 76 11 g g PROPN ct-9899 76 12 na2hpo4 na2hpo4 PROPN ct-9899 76 13 , , PUNCT ct-9899 76 14 4.3850 4.3850 NUM ct-9899 76 15 g g NOUN ct-9899 76 16 nacl nacl NOUN ct-9899 76 17 , , PUNCT ct-9899 76 18 0.1700 0.1700 NUM ct-9899 76 19 g g PROPN ct-9899 76 20 disodium disodium NOUN ct-9899 76 21 edta edta PROPN ct-9899 76 22 , , PUNCT ct-9899 76 23 q.s q.s PROPN ct-9899 76 24 . . PROPN ct-9899 76 25 500 500 NUM ct-9899 76 26 ml ml NOUN ct-9899 76 27 water water NOUN ct-9899 76 28 , , PUNCT ct-9899 76 29 ph ph NOUN ct-9899 76 30 6 6 NUM ct-9899 76 31 ) ) PUNCT ct-9899 76 32 was be AUX ct-9899 76 33 added add VERB ct-9899 76 34 and and CCONJ ct-9899 76 35 semen semen NOUN ct-9899 76 36 was be AUX ct-9899 76 37 incubated incubate VERB ct-9899 76 38 for for ADP ct-9899 76 39 3 3 NUM ct-9899 76 40 min min NOUN ct-9899 76 41 at at ADP ct-9899 76 42 room room NOUN ct-9899 76 43 temperature temperature NOUN ct-9899 76 44 . . PUNCT ct-9899 77 1 the the DET ct-9899 77 2 sample sample NOUN ct-9899 77 3 flow flow NOUN ct-9899 77 4 was be AUX ct-9899 77 5 initiated initiate VERB ct-9899 77 6 for for SCONJ ct-9899 77 7 1 1 NUM ct-9899 77 8 min min NOUN ct-9899 77 9 to to PART ct-9899 77 10 allow allow VERB ct-9899 77 11 for for ADP ct-9899 77 12 hydrodynamic hydrodynamic ADJ ct-9899 77 13 equilibration equilibration NOUN ct-9899 77 14 , , PUNCT ct-9899 77 15 after after ADP ct-9899 77 16 which which PRON ct-9899 77 17 data data NOUN ct-9899 77 18 collection collection NOUN ct-9899 77 19 was be AUX ct-9899 77 20 initiated initiate VERB ct-9899 77 21 . . PUNCT ct-9899 78 1 the the DET ct-9899 78 2 flow flow NOUN ct-9899 78 3 rate rate NOUN ct-9899 78 4 was be AUX ct-9899 78 5 adjusted adjust VERB ct-9899 78 6 to to ADP ct-9899 78 7 around around ADP ct-9899 78 8 200 200 NUM ct-9899 78 9 cells cell NOUN ct-9899 78 10 / / SYM ct-9899 78 11 sec sec PROPN ct-9899 78 12 . . PROPN ct-9899 79 1 from from ADP ct-9899 79 2 each each DET ct-9899 79 3 sample sample NOUN ct-9899 79 4 , , PUNCT ct-9899 79 5 20 20 NUM ct-9899 79 6 000 000 NUM ct-9899 79 7 events event NOUN ct-9899 79 8 in in ADP ct-9899 79 9 the the DET ct-9899 79 10 forward forward NOUN ct-9899 79 11 and and CCONJ ct-9899 79 12 90º 90º NUM ct-9899 79 13 light light NOUN ct-9899 79 14 scatter scatter NOUN ct-9899 79 15 population population NOUN ct-9899 79 16 representing represent VERB ct-9899 79 17 whole whole ADJ ct-9899 79 18 sperm sperm NOUN ct-9899 79 19 were be AUX ct-9899 79 20 analyzed analyze VERB ct-9899 79 21 using use VERB ct-9899 79 22 0.2-m 0.2-m PROPN ct-9899 79 23 filtered filter VERB ct-9899 79 24 18 18 NUM ct-9899 79 25 mega mega ADJ ct-9899 79 26 - - ADJ ct-9899 79 27 ohm ohm ADJ ct-9899 79 28 ultra ultra ADJ ct-9899 79 29 - - ADJ ct-9899 79 30 pure pure ADJ ct-9899 79 31 water water NOUN ct-9899 79 32 as as ADP ct-9899 79 33 the the DET ct-9899 79 34 sheath sheath NOUN ct-9899 79 35 fluid fluid NOUN ct-9899 79 36 . . PUNCT ct-9899 80 1 a a DET ct-9899 80 2 gate gate NOUN ct-9899 80 3 containing contain VERB ct-9899 80 4 sperm sperm NOUN ct-9899 80 5 was be AUX ct-9899 80 6 selected select VERB ct-9899 80 7 based base VERB ct-9899 80 8 on on ADP ct-9899 80 9 dot dot NOUN ct-9899 80 10 plot plot NOUN ct-9899 80 11 distribution distribution NOUN ct-9899 80 12 of of ADP ct-9899 80 13 forward forward ADV ct-9899 80 14 ( ( PUNCT ct-9899 80 15 size size NOUN ct-9899 80 16 ) ) PUNCT ct-9899 80 17 versus versus ADP ct-9899 80 18 side side NOUN ct-9899 80 19 scatter scatter NOUN ct-9899 80 20 ( ( PUNCT ct-9899 80 21 complexity complexity NOUN ct-9899 80 22 parameter parameter NOUN ct-9899 80 23 ) ) PUNCT ct-9899 80 24 to to PART ct-9899 80 25 eliminate eliminate VERB ct-9899 80 26 debris debris NOUN ct-9899 80 27 and and CCONJ ct-9899 80 28 somatic somatic ADJ ct-9899 80 29 cells cell NOUN ct-9899 80 30 from from ADP ct-9899 80 31 the the DET ct-9899 80 32 analysis analysis NOUN ct-9899 80 33 . . PUNCT ct-9899 81 1 the the DET ct-9899 81 2 green green ADJ ct-9899 81 3 and and CCONJ ct-9899 81 4 red red ADJ ct-9899 81 5 signals signal NOUN ct-9899 81 6 were be AUX ct-9899 81 7 detected detect VERB ct-9899 81 8 using use VERB ct-9899 81 9 a a DET ct-9899 81 10 5mwatt 5mwatt NUM ct-9899 81 11 blue blue ADJ ct-9899 81 12 argon argon NOUN ct-9899 81 13 laser laser NOUN ct-9899 81 14 ( ( PUNCT ct-9899 81 15 488 488 NUM ct-9899 81 16 nm nm NOUN ct-9899 81 17 ) ) PUNCT ct-9899 81 18 and and CCONJ ct-9899 81 19 emission emission NOUN ct-9899 81 20 filters filter NOUN ct-9899 81 21 ( ( PUNCT ct-9899 81 22 535 535 NUM ct-9899 81 23 ± ± NUM ct-9899 81 24 30 30 NUM ct-9899 81 25 nm nm NOUN ct-9899 81 26 for for ADP ct-9899 81 27 green green ADJ ct-9899 81 28 and and CCONJ ct-9899 81 29 585 585 NUM ct-9899 81 30 ± ± NUM ct-9899 81 31 30 30 NUM ct-9899 81 32 nm nm NOUN ct-9899 81 33 for for ADP ct-9899 81 34 red red NOUN ct-9899 81 35 ) ) PUNCT ct-9899 81 36 . . PUNCT ct-9899 82 1 baseline baseline PROPN ct-9899 82 2 background background PROPN ct-9899 82 3 fluorescence fluorescence PROPN ct-9899 82 4 signal signal NOUN ct-9899 82 5 was be AUX ct-9899 82 6 initially initially ADV ct-9899 82 7 evaluated evaluate VERB ct-9899 82 8 in in ADP ct-9899 82 9 unstained unstained ADJ ct-9899 82 10 samples sample NOUN ct-9899 82 11 . . PUNCT ct-9899 83 1 the the DET ct-9899 83 2 control control NOUN ct-9899 83 3 area area NOUN ct-9899 83 4 was be AUX ct-9899 83 5 marked mark VERB ct-9899 83 6 on on ADP ct-9899 83 7 unstained unstained ADJ ct-9899 83 8 samples sample NOUN ct-9899 83 9 with with ADP ct-9899 83 10 < < X ct-9899 83 11 1 1 NUM ct-9899 83 12 % % NOUN ct-9899 83 13 of of ADP ct-9899 83 14 cells cell NOUN ct-9899 83 15 registering register VERB ct-9899 83 16 as as ADP ct-9899 83 17 positive positive ADJ ct-9899 83 18 for for ADP ct-9899 83 19 both both DET ct-9899 83 20 signals signal NOUN ct-9899 83 21 . . PUNCT ct-9899 84 1 the the DET ct-9899 84 2 main main ADJ ct-9899 84 3 population population NOUN ct-9899 84 4 represented represent VERB ct-9899 84 5 sperm sperm NOUN ct-9899 84 6 that that PRON ct-9899 84 7 emitted emit VERB ct-9899 84 8 more more ADV ct-9899 84 9 green green ADJ ct-9899 84 10 than than ADP ct-9899 84 11 red red ADJ ct-9899 84 12 fluorescence fluorescence NOUN ct-9899 84 13 ( ( PUNCT ct-9899 84 14 native native ADJ ct-9899 84 15 double double ADJ ct-9899 84 16 - - PUNCT ct-9899 84 17 stranded strand VERB ct-9899 84 18 dna dna NOUN ct-9899 84 19 ) ) PUNCT ct-9899 84 20 . . PUNCT ct-9899 85 1 cells cell NOUN ct-9899 85 2 located locate VERB ct-9899 85 3 to to ADP ct-9899 85 4 the the DET ct-9899 85 5 right right NOUN ct-9899 85 6 and and CCONJ ct-9899 85 7 below below ADP ct-9899 85 8 the the DET ct-9899 85 9 main main ADJ ct-9899 85 10 421 421 NUM ct-9899 85 11 clinical clinical ADJ ct-9899 85 12 theriogenology theriogenology NOUN ct-9899 85 13 • • NOUN ct-9899 85 14 volume volume NOUN ct-9899 85 15 10 10 NUM ct-9899 85 16 , , PUNCT ct-9899 85 17 number number NOUN ct-9899 85 18 4 4 NUM ct-9899 85 19 • • NOUN ct-9899 85 20 december december PROPN ct-9899 85 21 2018 2018 NUM ct-9899 85 22 population population NOUN ct-9899 85 23 represented represent VERB ct-9899 85 24 sperm sperm NOUN ct-9899 85 25 with with ADP ct-9899 85 26 increased increase VERB ct-9899 85 27 red red ADJ ct-9899 85 28 fluorescence fluorescence NOUN ct-9899 85 29 and and CCONJ ct-9899 85 30 decreased decrease VERB ct-9899 85 31 green green ADJ ct-9899 85 32 fluorescence fluorescence NOUN ct-9899 85 33 ( ( PUNCT ct-9899 85 34 denatured denature VERB ct-9899 85 35 single single ADJ ct-9899 85 36 - - PUNCT ct-9899 85 37 stranded strand VERB ct-9899 85 38 dna dna NOUN ct-9899 85 39 ) ) PUNCT ct-9899 85 40 . . PUNCT ct-9899 86 1 a a DET ct-9899 86 2 modified modify VERB ct-9899 86 3 dna dna PROPN ct-9899 86 4 fragmentation fragmentation NOUN ct-9899 86 5 index index NOUN ct-9899 86 6 ( ( PUNCT ct-9899 86 7 mdfi mdfi PROPN ct-9899 86 8 ) ) PUNCT ct-9899 86 9 was be AUX ct-9899 86 10 calculated calculate VERB ct-9899 86 11 as as ADP ct-9899 86 12 the the DET ct-9899 86 13 percentage percentage NOUN ct-9899 86 14 of of ADP ct-9899 86 15 sperm sperm NOUN ct-9899 86 16 with with ADP ct-9899 86 17 denatured denature VERB ct-9899 86 18 dna dna PROPN ct-9899 86 19 ( ( PUNCT ct-9899 86 20 red red ADJ ct-9899 86 21 ) ) PUNCT ct-9899 86 22 divided divide VERB ct-9899 86 23 by by ADP ct-9899 86 24 the the DET ct-9899 86 25 percentage percentage NOUN ct-9899 86 26 of of ADP ct-9899 86 27 total total ADJ ct-9899 86 28 sperm sperm NOUN ct-9899 86 29 with with ADP ct-9899 86 30 fluorescence fluorescence NOUN ct-9899 86 31 emission emission NOUN ct-9899 86 32 ( ( PUNCT ct-9899 86 33 green green ADJ ct-9899 86 34 + + X ct-9899 86 35 red red ADJ ct-9899 86 36 ) ) PUNCT ct-9899 86 37 . . PUNCT ct-9899 87 1 statistical statistical ADJ ct-9899 87 2 analysis analysis NOUN ct-9899 87 3 statistical statistical ADJ ct-9899 87 4 analysis analysis NOUN ct-9899 87 5 was be AUX ct-9899 87 6 performed perform VERB ct-9899 87 7 using use VERB ct-9899 87 8 sas sas PROPN ct-9899 87 9 package package NOUN ct-9899 87 10 ( ( PUNCT ct-9899 87 11 sas sas PROPN ct-9899 87 12 institute institute PROPN ct-9899 87 13 , , PUNCT ct-9899 87 14 cary cary PROPN ct-9899 87 15 , , PUNCT ct-9899 87 16 nc nc PROPN ct-9899 87 17 , , PUNCT ct-9899 87 18 usa usa PROPN ct-9899 87 19 ) ) PUNCT ct-9899 87 20 . . PUNCT ct-9899 88 1 distribution distribution NOUN ct-9899 88 2 of of ADP ct-9899 88 3 the the DET ct-9899 88 4 data datum NOUN ct-9899 88 5 were be AUX ct-9899 88 6 tested test VERB ct-9899 88 7 for for ADP ct-9899 88 8 normality normality NOUN ct-9899 88 9 using use VERB ct-9899 88 10 a a DET ct-9899 88 11 shapiro shapiro PROPN ct-9899 88 12 wilk wilk PROPN ct-9899 88 13 test test NOUN ct-9899 88 14 . . PUNCT ct-9899 89 1 variables variable NOUN ct-9899 89 2 that that PRON ct-9899 89 3 did do AUX ct-9899 89 4 not not PART ct-9899 89 5 follow follow VERB ct-9899 89 6 a a DET ct-9899 89 7 normal normal ADJ ct-9899 89 8 distribution distribution NOUN ct-9899 89 9 underwent undergo VERB ct-9899 89 10 logarithmic logarithmic ADJ ct-9899 89 11 transformation transformation NOUN ct-9899 89 12 . . PUNCT ct-9899 90 1 means mean NOUN ct-9899 90 2 were be AUX ct-9899 90 3 compared compare VERB ct-9899 90 4 between between ADP ct-9899 90 5 treatments treatment NOUN ct-9899 90 6 using use VERB ct-9899 90 7 anova anova PROPN ct-9899 90 8 , , PUNCT ct-9899 90 9 including include VERB ct-9899 90 10 the the DET ct-9899 90 11 fixed fix VERB ct-9899 90 12 effect effect NOUN ct-9899 90 13 of of ADP ct-9899 90 14 treatment treatment NOUN ct-9899 90 15 . . PUNCT ct-9899 91 1 paired pair VERB ct-9899 91 2 comparisons comparison NOUN ct-9899 91 3 were be AUX ct-9899 91 4 made make VERB ct-9899 91 5 using use VERB ct-9899 91 6 a a DET ct-9899 91 7 tukey tukey NOUN ct-9899 91 8 ’s ’s PART ct-9899 91 9 test test NOUN ct-9899 91 10 . . PUNCT ct-9899 92 1 data datum NOUN ct-9899 92 2 were be AUX ct-9899 92 3 expressed express VERB ct-9899 92 4 as as ADP ct-9899 92 5 mean mean NOUN ct-9899 92 6   PROPN ct-9899 92 7 se se X ct-9899 92 8 and and CCONJ ct-9899 92 9 differences difference NOUN ct-9899 92 10 were be AUX ct-9899 92 11 considered consider VERB ct-9899 92 12 significant significant ADJ ct-9899 92 13 if if SCONJ ct-9899 92 14 p p X ct-9899 92 15 < < X ct-9899 92 16 0.05 0.05 NUM ct-9899 92 17 . . PUNCT ct-9899 93 1 results result NOUN ct-9899 93 2 sperm sperm NOUN ct-9899 93 3 were be AUX ct-9899 93 4 obtained obtain VERB ct-9899 93 5 from from ADP ct-9899 93 6 70 70 NUM ct-9899 93 7 % % NOUN ct-9899 93 8 ( ( PUNCT ct-9899 93 9 7/10 7/10 NUM ct-9899 93 10 ) ) PUNCT ct-9899 93 11 of of ADP ct-9899 93 12 the the DET ct-9899 93 13 epididymides epididymis NOUN ct-9899 93 14 processed process VERB ct-9899 93 15 on on ADP ct-9899 93 16 the the DET ct-9899 93 17 day day NOUN ct-9899 93 18 of of ADP ct-9899 93 19 castration castration NOUN ct-9899 93 20 , , PUNCT ct-9899 93 21 and and CCONJ ct-9899 93 22 60 60 NUM ct-9899 93 23 % % NOUN ct-9899 93 24 ( ( PUNCT ct-9899 93 25 6/10 6/10 NUM ct-9899 93 26 ) ) PUNCT ct-9899 93 27 of of ADP ct-9899 93 28 those those PRON ct-9899 93 29 processed process VERB ct-9899 93 30 after after ADP ct-9899 93 31 24 24 NUM ct-9899 93 32 h h NOUN ct-9899 93 33 of of ADP ct-9899 93 34 cold cold ADJ ct-9899 93 35 storage storage NOUN ct-9899 93 36 . . PUNCT ct-9899 94 1 pre pre ADJ ct-9899 94 2 - - ADJ ct-9899 94 3 freezing freezing ADJ ct-9899 94 4 sperm sperm NOUN ct-9899 94 5 parameters parameter NOUN ct-9899 94 6 did do AUX ct-9899 94 7 not not PART ct-9899 94 8 differ differ VERB ct-9899 94 9 between between ADP ct-9899 94 10 the the DET ct-9899 94 11 samples sample NOUN ct-9899 94 12 obtained obtain VERB ct-9899 94 13 on on ADP ct-9899 94 14 the the DET ct-9899 94 15 day day NOUN ct-9899 94 16 of of ADP ct-9899 94 17 castration castration NOUN ct-9899 94 18 or or CCONJ ct-9899 94 19 24 24 NUM ct-9899 94 20 h h NOUN ct-9899 94 21 later later ADV ct-9899 94 22 ( ( PUNCT ct-9899 94 23 p p X ct-9899 94 24 > > X ct-9899 94 25 0.05 0.05 NUM ct-9899 94 26 ; ; PUNCT ct-9899 94 27 table table NOUN ct-9899 94 28 1 1 NUM ct-9899 94 29 ) ) PUNCT ct-9899 94 30 . . PUNCT ct-9899 95 1 the the DET ct-9899 95 2 proportion proportion NOUN ct-9899 95 3 of of ADP ct-9899 95 4 pre pre ADJ ct-9899 95 5 - - ADJ ct-9899 95 6 freezing freezing ADJ ct-9899 95 7 total total ADJ ct-9899 95 8 and and CCONJ ct-9899 95 9 progressive progressive ADJ ct-9899 95 10 motility motility NOUN ct-9899 95 11 retained retain VERB ct-9899 95 12 ( ( PUNCT ct-9899 95 13 % % NOUN ct-9899 95 14 of of ADP ct-9899 95 15 initial initial ADJ ct-9899 95 16 motility motility NOUN ct-9899 95 17 ) ) PUNCT ct-9899 95 18 after after ADP ct-9899 95 19 24 24 NUM ct-9899 95 20 h h NOUN ct-9899 95 21 of of ADP ct-9899 95 22 cold cold ADJ ct-9899 95 23 storage storage NOUN ct-9899 95 24 was be AUX ct-9899 95 25 65.1±10.9 65.1±10.9 NUM ct-9899 95 26 % % NOUN ct-9899 95 27 and and CCONJ ct-9899 95 28 49.9±16.1 49.9±16.1 NUM ct-9899 95 29 % % NOUN ct-9899 95 30 , , PUNCT ct-9899 95 31 respectively respectively ADV ct-9899 95 32 . . PUNCT ct-9899 96 1 motility motility NOUN ct-9899 96 2 of of ADP ct-9899 96 3 sperm sperm NOUN ct-9899 96 4 cryopreserved cryopreserve VERB ct-9899 96 5 in in ADP ct-9899 96 6 botucrio botucrio NOUN ct-9899 96 7 on on ADP ct-9899 96 8 the the DET ct-9899 96 9 day day NOUN ct-9899 96 10 of of ADP ct-9899 96 11 castration castration NOUN ct-9899 96 12 did do AUX ct-9899 96 13 not not PART ct-9899 96 14 differ differ VERB ct-9899 96 15 from from ADP ct-9899 96 16 prefreezing prefreeze VERB ct-9899 96 17 values value NOUN ct-9899 96 18 . . PUNCT ct-9899 97 1 however however ADV ct-9899 97 2 , , PUNCT ct-9899 97 3 total total NOUN ct-9899 97 4 ( ( PUNCT ct-9899 97 5 p p X ct-9899 97 6 = = NOUN ct-9899 97 7 0.002 0.002 NUM ct-9899 97 8 ) ) PUNCT ct-9899 97 9 and and CCONJ ct-9899 97 10 progressive progressive ADJ ct-9899 97 11 ( ( PUNCT ct-9899 97 12 p p X ct-9899 97 13 = = NOUN ct-9899 97 14 0.041 0.041 NUM ct-9899 97 15 ) ) PUNCT ct-9899 97 16 sperm sperm NOUN ct-9899 97 17 motility motility NOUN ct-9899 97 18 were be AUX ct-9899 97 19 lower low ADJ ct-9899 97 20 when when SCONJ ct-9899 97 21 optixcell optixcell NOUN ct-9899 97 22 was be AUX ct-9899 97 23 used use VERB ct-9899 97 24 or or CCONJ ct-9899 97 25 cryopreservation cryopreservation NOUN ct-9899 97 26 was be AUX ct-9899 97 27 delayed delay VERB ct-9899 97 28 for for ADP ct-9899 97 29 24 24 NUM ct-9899 97 30 h h NOUN ct-9899 97 31 ( ( PUNCT ct-9899 97 32 figure figure NOUN ct-9899 97 33 ) ) PUNCT ct-9899 97 34 . . PUNCT ct-9899 98 1 post post ADJ ct-9899 98 2 - - ADJ ct-9899 98 3 thaw thaw ADJ ct-9899 98 4 membrane membrane NOUN ct-9899 98 5 integrity integrity NOUN ct-9899 98 6 was be AUX ct-9899 98 7 also also ADV ct-9899 98 8 affected affect VERB ct-9899 98 9 by by ADP ct-9899 98 10 treatment treatment NOUN ct-9899 98 11 and and CCONJ ct-9899 98 12 was be AUX ct-9899 98 13 lowest low ADJ ct-9899 98 14 when when SCONJ ct-9899 98 15 sperm sperm NOUN ct-9899 98 16 were be AUX ct-9899 98 17 cryopreserved cryopreserve VERB ct-9899 98 18 in in ADP ct-9899 98 19 optixcell optixcell NOUN ct-9899 98 20 after after ADP ct-9899 98 21 cold cold ADJ ct-9899 98 22 storage storage NOUN ct-9899 98 23 ( ( PUNCT ct-9899 98 24 p p NOUN ct-9899 98 25 = = NOUN ct-9899 98 26 0.039 0.039 NUM ct-9899 98 27 ; ; PUNCT ct-9899 98 28 figure figure NOUN ct-9899 98 29 ) ) PUNCT ct-9899 98 30 . . PUNCT ct-9899 99 1 no no DET ct-9899 99 2 other other ADJ ct-9899 99 3 sperm sperm NOUN ct-9899 99 4 motion motion NOUN ct-9899 99 5 parameters parameter NOUN ct-9899 99 6 differed differ VERB ct-9899 99 7 with with ADP ct-9899 99 8 treatment treatment NOUN ct-9899 99 9 ( ( PUNCT ct-9899 99 10 table table NOUN ct-9899 99 11 2 2 NUM ct-9899 99 12 ) ) PUNCT ct-9899 99 13 . . PUNCT ct-9899 100 1 similarly similarly ADV ct-9899 100 2 , , PUNCT ct-9899 100 3 post post ADJ ct-9899 100 4 - - ADJ ct-9899 100 5 thaw thaw ADJ ct-9899 100 6 mdfi mdfi NOUN ct-9899 100 7 did do AUX ct-9899 100 8 not not PART ct-9899 100 9 differ differ VERB ct-9899 100 10 with with ADP ct-9899 100 11 treatment treatment NOUN ct-9899 100 12 . . PUNCT ct-9899 101 1 mean mean PROPN ct-9899 101 2 mdfi mdfi PROPN ct-9899 101 3 was be AUX ct-9899 101 4 2.5 2.5 NUM ct-9899 101 5   PROPN ct-9899 101 6 1.8 1.8 NUM ct-9899 101 7 % % NOUN ct-9899 101 8 and and CCONJ ct-9899 101 9 4.3 4.3 NUM ct-9899 101 10   PROPN ct-9899 101 11 3.1 3.1 NUM ct-9899 101 12 % % NOUN ct-9899 101 13 for for ADP ct-9899 101 14 sperm sperm NOUN ct-9899 101 15 cryopreserved cryopreserve VERB ct-9899 101 16 immediately immediately ADV ct-9899 101 17 in in ADP ct-9899 101 18 botucrio botucrio NOUN ct-9899 101 19 or or CCONJ ct-9899 101 20 optixcell optixcell NOUN ct-9899 101 21 , , PUNCT ct-9899 101 22 respectively respectively ADV ct-9899 101 23 ; ; PUNCT ct-9899 101 24 and and CCONJ ct-9899 101 25 3.3 3.3 NUM ct-9899 101 26   PROPN ct-9899 101 27 1.8 1.8 NUM ct-9899 101 28 % % NOUN ct-9899 101 29 and and CCONJ ct-9899 101 30 4.1 4.1 NUM ct-9899 101 31   PROPN ct-9899 101 32 3.1 3.1 NUM ct-9899 101 33 % % NOUN ct-9899 101 34 for for ADP ct-9899 101 35 sperm sperm NOUN ct-9899 101 36 cryopreserved cryopreserve VERB ct-9899 101 37 after after ADP ct-9899 101 38 cold cold ADJ ct-9899 101 39 storage storage NOUN ct-9899 101 40 in in ADP ct-9899 101 41 botucrio botucrio NOUN ct-9899 101 42 or or CCONJ ct-9899 101 43 optixcell optixcell NOUN ct-9899 101 44 , , PUNCT ct-9899 101 45 respectively respectively ADV ct-9899 101 46 . . PUNCT ct-9899 102 1 discussion discussion NOUN ct-9899 102 2 to to ADP ct-9899 102 3 the the DET ct-9899 102 4 authors author NOUN ct-9899 102 5 ’ ’ PART ct-9899 102 6 knowledge knowledge NOUN ct-9899 102 7 , , PUNCT ct-9899 102 8 this this PRON ct-9899 102 9 is be AUX ct-9899 102 10 the the DET ct-9899 102 11 first first ADJ ct-9899 102 12 study study NOUN ct-9899 102 13 directly directly ADV ct-9899 102 14 comparing compare VERB ct-9899 102 15 post post ADJ ct-9899 102 16 - - ADJ ct-9899 102 17 thaw thaw ADJ ct-9899 102 18 quality quality NOUN ct-9899 102 19 of of ADP ct-9899 102 20 alpaca alpaca NOUN ct-9899 102 21 epididymal epididymal ADJ ct-9899 102 22 sperm sperm NOUN ct-9899 102 23 recovered recover VERB ct-9899 102 24 on on ADP ct-9899 102 25 the the DET ct-9899 102 26 day day NOUN ct-9899 102 27 of of ADP ct-9899 102 28 castration castration NOUN ct-9899 102 29 and and CCONJ ct-9899 102 30 after after ADP ct-9899 102 31 24 24 NUM ct-9899 102 32 h h NOUN ct-9899 102 33 of of ADP ct-9899 102 34 cold cold ADJ ct-9899 102 35 storage storage NOUN ct-9899 102 36 . . PUNCT ct-9899 103 1 without without ADP ct-9899 103 2 direct direct ADJ ct-9899 103 3 comparisons comparison NOUN ct-9899 103 4 between between ADP ct-9899 103 5 holding hold VERB ct-9899 103 6 times time NOUN ct-9899 103 7 , , PUNCT ct-9899 103 8 recommendations recommendation NOUN ct-9899 103 9 for for ADP ct-9899 103 10 immediate immediate ADJ ct-9899 103 11 or or CCONJ ct-9899 103 12 delayed delay VERB ct-9899 103 13 processing processing NOUN ct-9899 103 14 of of ADP ct-9899 103 15 alpaca alpaca NOUN ct-9899 103 16 epididymides epididymis NOUN ct-9899 103 17 could could AUX ct-9899 103 18 not not PART ct-9899 103 19 be be AUX ct-9899 103 20 made make VERB ct-9899 103 21 . . PUNCT ct-9899 104 1 while while SCONJ ct-9899 104 2 mean mean VERB ct-9899 104 3 sperm sperm NOUN ct-9899 104 4 motility motility NOUN ct-9899 104 5 in in ADP ct-9899 104 6 this this DET ct-9899 104 7 study study NOUN ct-9899 104 8 was be AUX ct-9899 104 9 not not PART ct-9899 104 10 statistically statistically ADV ct-9899 104 11 different different ADJ ct-9899 104 12 between between ADP ct-9899 104 13 storage storage NOUN ct-9899 104 14 times time NOUN ct-9899 104 15 , , PUNCT ct-9899 104 16 storage storage NOUN ct-9899 104 17 of of ADP ct-9899 104 18 testes testis NOUN ct-9899 104 19 with with ADP ct-9899 104 20 epididymides epididymis NOUN ct-9899 104 21 at at ADP ct-9899 104 22 4c 4c PROPN ct-9899 104 23 for for ADP ct-9899 104 24 24 24 NUM ct-9899 104 25 h h NOUN ct-9899 104 26 resulted result VERB ct-9899 104 27 in in ADP ct-9899 104 28 loss loss NOUN ct-9899 104 29 of of ADP ct-9899 104 30 35 35 NUM ct-9899 104 31 % % NOUN ct-9899 104 32 and and CCONJ ct-9899 104 33 51 51 NUM ct-9899 104 34 % % NOUN ct-9899 104 35 of of ADP ct-9899 104 36 total total ADJ ct-9899 104 37 and and CCONJ ct-9899 104 38 progressively progressively ADV ct-9899 104 39 motile motile ADJ ct-9899 104 40 sperm sperm NOUN ct-9899 104 41 , , PUNCT ct-9899 104 42 respectively respectively ADV ct-9899 104 43 . . PUNCT ct-9899 105 1 moreover moreover ADV ct-9899 105 2 , , PUNCT ct-9899 105 3 post post ADJ ct-9899 105 4 - - ADJ ct-9899 105 5 thaw thaw ADJ ct-9899 105 6 motility motility NOUN ct-9899 105 7 was be AUX ct-9899 105 8 significantly significantly ADV ct-9899 105 9 decreased decrease VERB ct-9899 105 10 by by ADP ct-9899 105 11 cold cold ADJ ct-9899 105 12 storage storage NOUN ct-9899 105 13 compared compare VERB ct-9899 105 14 with with ADP ct-9899 105 15 pre pre ADJ ct-9899 105 16 - - ADJ ct-9899 105 17 freezing freezing ADJ ct-9899 105 18 values value NOUN ct-9899 105 19 of of ADP ct-9899 105 20 sperm sperm NOUN ct-9899 105 21 recovered recover VERB ct-9899 105 22 on on ADP ct-9899 105 23 the the DET ct-9899 105 24 day day NOUN ct-9899 105 25 of of ADP ct-9899 105 26 castration castration NOUN ct-9899 105 27 . . PUNCT ct-9899 106 1 while while SCONJ ct-9899 106 2 the the DET ct-9899 106 3 ability ability NOUN ct-9899 106 4 to to PART ct-9899 106 5 preserve preserve VERB ct-9899 106 6 sperm sperm NOUN ct-9899 106 7 viability viability NOUN ct-9899 106 8 for for ADP ct-9899 106 9 a a DET ct-9899 106 10 prolonged prolonged ADJ ct-9899 106 11 time time NOUN ct-9899 106 12 would would AUX ct-9899 106 13 allow allow VERB ct-9899 106 14 practitioners practitioner NOUN ct-9899 106 15 to to PART ct-9899 106 16 ship ship VERB ct-9899 106 17 the the DET ct-9899 106 18 epididymides epididymis NOUN ct-9899 106 19 of of ADP ct-9899 106 20 deceased deceased ADJ ct-9899 106 21 males male NOUN ct-9899 106 22 to to ADP ct-9899 106 23 a a DET ct-9899 106 24 centralized centralized ADJ ct-9899 106 25 facility facility NOUN ct-9899 106 26 for for ADP ct-9899 106 27 sperm sperm NOUN ct-9899 106 28 recovery recovery NOUN ct-9899 106 29 and and CCONJ ct-9899 106 30 cryopreservation cryopreservation NOUN ct-9899 106 31 , , PUNCT ct-9899 106 32 based base VERB ct-9899 106 33 on on ADP ct-9899 106 34 the the DET ct-9899 106 35 results result NOUN ct-9899 106 36 of of ADP ct-9899 106 37 this this DET ct-9899 106 38 study study NOUN ct-9899 106 39 , , PUNCT ct-9899 106 40 this this DET ct-9899 106 41 practice practice NOUN ct-9899 106 42 is be AUX ct-9899 106 43 not not PART ct-9899 106 44 recommended recommend VERB ct-9899 106 45 in in ADP ct-9899 106 46 alpacas alpaca NOUN ct-9899 106 47 . . PUNCT ct-9899 107 1 this this PRON ct-9899 107 2 is be AUX ct-9899 107 3 in in ADP ct-9899 107 4 contrast contrast NOUN ct-9899 107 5 with with ADP ct-9899 107 6 findings finding NOUN ct-9899 107 7 in in ADP ct-9899 107 8 other other ADJ ct-9899 107 9 species specie NOUN ct-9899 107 10 . . PUNCT ct-9899 108 1 in in ADP ct-9899 108 2 stallions stallion NOUN ct-9899 108 3 and and CCONJ ct-9899 108 4 bucks buck NOUN ct-9899 108 5 , , PUNCT ct-9899 108 6 sperm sperm NOUN ct-9899 108 7 viability viability NOUN ct-9899 108 8 and and CCONJ ct-9899 108 9 fertility fertility NOUN ct-9899 108 10 were be AUX ct-9899 108 11 maintained maintain VERB ct-9899 108 12 when when SCONJ ct-9899 108 13 epididymides epididymis NOUN ct-9899 108 14 were be AUX ct-9899 108 15 cooled cool VERB ct-9899 108 16 for for ADP ct-9899 108 17 48 48 NUM ct-9899 108 18 to to PART ct-9899 108 19 96 96 NUM ct-9899 108 20 h.9,10,13 h.9,10,13 PROPN ct-9899 108 21 differences difference NOUN ct-9899 108 22 in in ADP ct-9899 108 23 diluent diluent NOUN ct-9899 108 24 , , PUNCT ct-9899 108 25 cryoprotectant cryoprotectant NOUN ct-9899 108 26 or or CCONJ ct-9899 108 27 sperm sperm NOUN ct-9899 108 28 membrane membrane NOUN ct-9899 108 29 composition composition NOUN ct-9899 108 30 may may AUX ct-9899 108 31 account account VERB ct-9899 108 32 for for ADP ct-9899 108 33 the the DET ct-9899 108 34 discrepancies discrepancy NOUN ct-9899 108 35 among among ADP ct-9899 108 36 species specie NOUN ct-9899 108 37 . . PUNCT ct-9899 109 1 the the DET ct-9899 109 2 osmolality osmolality NOUN ct-9899 109 3 of of ADP ct-9899 109 4 the the DET ct-9899 109 5 semen semen NOUN ct-9899 109 6 extender extender NOUN ct-9899 109 7 was be AUX ct-9899 109 8 shown show VERB ct-9899 109 9 to to PART ct-9899 109 10 affect affect VERB ct-9899 109 11 survival survival NOUN ct-9899 109 12 of of ADP ct-9899 109 13 cold cold ADJ ct-9899 109 14 - - PUNCT ct-9899 109 15 stored store VERB ct-9899 109 16 ram ram NOUN ct-9899 109 17 epididymal epididymal ADJ ct-9899 109 18 sperm.14 sperm.14 NOUN ct-9899 109 19 it it PRON ct-9899 109 20 was be AUX ct-9899 109 21 proposed propose VERB ct-9899 109 22 that that SCONJ ct-9899 109 23 since since SCONJ ct-9899 109 24 the the DET ct-9899 109 25 osmolality osmolality NOUN ct-9899 109 26 of of ADP ct-9899 109 27 the the DET ct-9899 109 28 epididymal epididymal ADJ ct-9899 109 29 medium medium ADJ ct-9899 109 30 increases increase NOUN ct-9899 109 31 with with ADP ct-9899 109 32 post post ADJ ct-9899 109 33 - - ADJ ct-9899 109 34 mortem mortem ADJ ct-9899 109 35 time time NOUN ct-9899 109 36 , , PUNCT ct-9899 109 37 epididymal epididymal ADJ ct-9899 109 38 sperm sperm NOUN ct-9899 109 39 might might AUX ct-9899 109 40 adapt adapt VERB ct-9899 109 41 to to ADP ct-9899 109 42 an an DET ct-9899 109 43 increasingly increasingly ADV ct-9899 109 44 hypertonic hypertonic ADJ ct-9899 109 45 environment environment NOUN ct-9899 109 46 , , PUNCT ct-9899 109 47 requiring require VERB ct-9899 109 48 the the DET ct-9899 109 49 use use NOUN ct-9899 109 50 of of ADP ct-9899 109 51 semen semen NOUN ct-9899 109 52 extenders extender NOUN ct-9899 109 53 with with ADP ct-9899 109 54 higher high ADJ ct-9899 109 55 osmolality osmolality NOUN ct-9899 109 56 for for ADP ct-9899 109 57 best good ADJ ct-9899 109 58 response response NOUN ct-9899 109 59 to to ADP ct-9899 109 60 cryopreservation.14 cryopreservation.14 NOUN ct-9899 109 61 the the DET ct-9899 109 62 effect effect NOUN ct-9899 109 63 of of ADP ct-9899 109 64 storage storage NOUN ct-9899 109 65 time time NOUN ct-9899 109 66 on on ADP ct-9899 109 67 osmolality osmolality NOUN ct-9899 109 68 of of ADP ct-9899 109 69 epididymal epididymal ADJ ct-9899 109 70 contents content NOUN ct-9899 109 71 recovered recover VERB ct-9899 109 72 postcastration postcastration NOUN ct-9899 109 73 , , PUNCT ct-9899 109 74 and and CCONJ ct-9899 109 75 the the DET ct-9899 109 76 effect effect NOUN ct-9899 109 77 of of ADP ct-9899 109 78 osmolality osmolality NOUN ct-9899 109 79 of of ADP ct-9899 109 80 semen semen NOUN ct-9899 109 81 extender extender NOUN ct-9899 109 82 on on ADP ct-9899 109 83 post post ADJ ct-9899 109 84 - - ADJ ct-9899 109 85 thaw thaw ADJ ct-9899 109 86 viability viability NOUN ct-9899 109 87 of of ADP ct-9899 109 88 alpaca alpaca NOUN ct-9899 109 89 sperm sperm NOUN ct-9899 109 90 require require VERB ct-9899 109 91 investigation investigation NOUN ct-9899 109 92 . . PUNCT ct-9899 110 1 this this DET ct-9899 110 2 study study NOUN ct-9899 110 3 was be AUX ct-9899 110 4 also also ADV ct-9899 110 5 first first ADJ ct-9899 110 6 to to PART ct-9899 110 7 evaluate evaluate VERB ct-9899 110 8 the the DET ct-9899 110 9 ability ability NOUN ct-9899 110 10 of of ADP ct-9899 110 11 commercial commercial ADJ ct-9899 110 12 semen semen NOUN ct-9899 110 13 extenders extender NOUN ct-9899 110 14 containing contain VERB ct-9899 110 15 dmfa dmfa NOUN ct-9899 110 16 or or CCONJ ct-9899 110 17 liposomes liposome NOUN ct-9899 110 18 to to PART ct-9899 110 19 support support VERB ct-9899 110 20 cryosurvival cryosurvival NOUN ct-9899 110 21 of of ADP ct-9899 110 22 alpaca alpaca NOUN ct-9899 110 23 sperm sperm NOUN ct-9899 110 24 . . PUNCT ct-9899 111 1 the the DET ct-9899 111 2 use use NOUN ct-9899 111 3 of of ADP ct-9899 111 4 a a DET ct-9899 111 5 diluent diluent NOUN ct-9899 111 6 containing contain VERB ct-9899 111 7 dmfa dmfa NOUN ct-9899 111 8 provided provide VERB ct-9899 111 9 the the DET ct-9899 111 10 best good ADJ ct-9899 111 11 preservation preservation NOUN ct-9899 111 12 of of ADP ct-9899 111 13 post post ADJ ct-9899 111 14 - - ADJ ct-9899 111 15 thaw thaw ADJ ct-9899 111 16 motility motility NOUN ct-9899 111 17 . . PUNCT ct-9899 112 1 the the DET ct-9899 112 2 liposome liposome NOUN ct-9899 112 3 - - PUNCT ct-9899 112 4 containing contain VERB ct-9899 112 5 semen semen NOUN ct-9899 112 6 extender extender NOUN ct-9899 112 7 was be AUX ct-9899 112 8 less less ADV ct-9899 112 9 suitable suitable ADJ ct-9899 112 10 for for ADP ct-9899 112 11 422clinical 422clinical ADJ ct-9899 112 12 theriogenology theriogenology NOUN ct-9899 112 13 • • NUM ct-9899 112 14 volume volume NOUN ct-9899 112 15 10 10 NUM ct-9899 112 16 , , PUNCT ct-9899 112 17 number number NOUN ct-9899 112 18 4 4 NUM ct-9899 112 19 • • NOUN ct-9899 112 20 december december PROPN ct-9899 112 21 2018 2018 NUM ct-9899 112 22 cryopreservation cryopreservation NOUN ct-9899 112 23 of of ADP ct-9899 112 24 alpaca alpaca NOUN ct-9899 112 25 epididymal epididymal ADJ ct-9899 112 26 sperm sperm NOUN ct-9899 112 27 , , PUNCT ct-9899 112 28 resulting result VERB ct-9899 112 29 in in ADP ct-9899 112 30 decreased decrease VERB ct-9899 112 31 post post ADJ ct-9899 112 32 - - ADJ ct-9899 112 33 thaw thaw ADJ ct-9899 112 34 motility motility NOUN ct-9899 112 35 and and CCONJ ct-9899 112 36 membrane membrane NOUN ct-9899 112 37 integrity integrity NOUN ct-9899 112 38 at at ADP ct-9899 112 39 both both DET ct-9899 112 40 processing processing NOUN ct-9899 112 41 times time NOUN ct-9899 112 42 . . PUNCT ct-9899 113 1 adding add VERB ct-9899 113 2 dmfa dmfa NOUN ct-9899 113 3 to to ADP ct-9899 113 4 freezing freeze VERB ct-9899 113 5 extenders extender NOUN ct-9899 113 6 also also ADV ct-9899 113 7 had have VERB ct-9899 113 8 a a DET ct-9899 113 9 beneficial beneficial ADJ ct-9899 113 10 effect effect NOUN ct-9899 113 11 on on ADP ct-9899 113 12 post post ADJ ct-9899 113 13 - - ADJ ct-9899 113 14 thaw thaw ADJ ct-9899 113 15 motility motility NOUN ct-9899 113 16 of of ADP ct-9899 113 17 stallion stallion NOUN ct-9899 113 18 epididymal epididymal ADJ ct-9899 113 19 sperm.7 sperm.7 NOUN ct-9899 113 20 this this PRON ct-9899 113 21 is be AUX ct-9899 113 22 thought think VERB ct-9899 113 23 to to PART ct-9899 113 24 be be AUX ct-9899 113 25 because because SCONJ ct-9899 113 26 dmfa dmfa NOUN ct-9899 113 27 is be AUX ct-9899 113 28 a a DET ct-9899 113 29 small small ADJ ct-9899 113 30 hydrophilic hydrophilic ADJ ct-9899 113 31 molecule molecule NOUN ct-9899 113 32 with with ADP ct-9899 113 33 good good ADJ ct-9899 113 34 cell cell NOUN ct-9899 113 35 penetration penetration NOUN ct-9899 113 36 and and CCONJ ct-9899 113 37 low low ADJ ct-9899 113 38 cytotoxicity.4,5 cytotoxicity.4,5 NOUN ct-9899 113 39 in in ADP ct-9899 113 40 this this DET ct-9899 113 41 study study NOUN ct-9899 113 42 , , PUNCT ct-9899 113 43 sperm sperm NOUN ct-9899 113 44 were be AUX ct-9899 113 45 recovered recover VERB ct-9899 113 46 from from ADP ct-9899 113 47 60 60 NUM ct-9899 113 48 to to PART ct-9899 113 49 70 70 NUM ct-9899 113 50 % % NOUN ct-9899 113 51 of of ADP ct-9899 113 52 the the DET ct-9899 113 53 epididymides epididymis NOUN ct-9899 113 54 . . PUNCT ct-9899 114 1 this this DET ct-9899 114 2 number number NOUN ct-9899 114 3 was be AUX ct-9899 114 4 lower low ADJ ct-9899 114 5 than than SCONJ ct-9899 114 6 previously previously ADV ct-9899 114 7 reported report VERB ct-9899 114 8 ( ( PUNCT ct-9899 114 9 90 90 NUM ct-9899 114 10 - - SYM ct-9899 114 11 100 100 NUM ct-9899 114 12 % % NOUN ct-9899 114 13 ) ) PUNCT ct-9899 114 14 . . PUNCT ct-9899 115 1 previous previous ADJ ct-9899 115 2 studies study NOUN ct-9899 115 3 have have AUX ct-9899 115 4 included include VERB ct-9899 115 5 animals animal NOUN ct-9899 115 6 of of ADP ct-9899 115 7 2 2 NUM ct-9899 115 8 to to PART ct-9899 115 9 4 4 NUM ct-9899 115 10 y y NOUN ct-9899 115 11 of of ADP ct-9899 115 12 age age NOUN ct-9899 115 13 and and CCONJ ct-9899 115 14 testicular testicular ADJ ct-9899 115 15 size size NOUN ct-9899 115 16 was be AUX ct-9899 115 17 a a DET ct-9899 115 18 criterion criterion NOUN ct-9899 115 19 for for ADP ct-9899 115 20 inclusion inclusion NOUN ct-9899 115 21 in in ADP ct-9899 115 22 the the DET ct-9899 115 23 study study NOUN ct-9899 115 24 . . PUNCT ct-9899 116 1 males male NOUN ct-9899 116 2 in in ADP ct-9899 116 3 this this DET ct-9899 116 4 study study NOUN ct-9899 116 5 were be AUX ct-9899 116 6 between between ADP ct-9899 116 7 1 1 NUM ct-9899 116 8 and and CCONJ ct-9899 116 9 7 7 NUM ct-9899 116 10 y y PROPN ct-9899 116 11 old old ADJ ct-9899 116 12 . . PUNCT ct-9899 117 1 no no DET ct-9899 117 2 attempt attempt NOUN ct-9899 117 3 was be AUX ct-9899 117 4 made make VERB ct-9899 117 5 to to PART ct-9899 117 6 identify identify VERB ct-9899 117 7 the the DET ct-9899 117 8 donor donor NOUN ct-9899 117 9 of of ADP ct-9899 117 10 each each DET ct-9899 117 11 tissue tissue NOUN ct-9899 117 12 or or CCONJ ct-9899 117 13 measure measure VERB ct-9899 117 14 testicular testicular ADJ ct-9899 117 15 size size NOUN ct-9899 117 16 . . PUNCT ct-9899 118 1 it it PRON ct-9899 118 2 is be AUX ct-9899 118 3 possible possible ADJ ct-9899 118 4 that that SCONJ ct-9899 118 5 the the DET ct-9899 118 6 epididymides epididymis NOUN ct-9899 118 7 that that PRON ct-9899 118 8 yielded yield VERB ct-9899 118 9 no no DET ct-9899 118 10 sperm sperm NOUN ct-9899 118 11 belonged belong VERB ct-9899 118 12 to to ADP ct-9899 118 13 young young ADJ ct-9899 118 14 peri peri NOUN ct-9899 118 15 - - PUNCT ct-9899 118 16 pubertal pubertal ADJ ct-9899 118 17 animals animal NOUN ct-9899 118 18 . . PUNCT ct-9899 119 1 on on ADP ct-9899 119 2 the the DET ct-9899 119 3 other other ADJ ct-9899 119 4 hand hand NOUN ct-9899 119 5 , , PUNCT ct-9899 119 6 the the DET ct-9899 119 7 number number NOUN ct-9899 119 8 of of ADP ct-9899 119 9 sperm sperm NOUN ct-9899 119 10 recovered recover VERB ct-9899 119 11 from from ADP ct-9899 119 12 each each DET ct-9899 119 13 male male NOUN ct-9899 119 14 that that PRON ct-9899 119 15 did do AUX ct-9899 119 16 yield yield VERB ct-9899 119 17 a a DET ct-9899 119 18 sample sample NOUN ct-9899 119 19 was be AUX ct-9899 119 20 higher high ADJ ct-9899 119 21 than than SCONJ ct-9899 119 22 previously previously ADV ct-9899 119 23 reported report VERB ct-9899 119 24 . . PUNCT ct-9899 120 1 reported report VERB ct-9899 120 2 mean mean PROPN ct-9899 120 3 recovery recovery NOUN ct-9899 120 4 ranged range VERB ct-9899 120 5 from from ADP ct-9899 120 6 75.3 75.3 NUM ct-9899 120 7 to to ADP ct-9899 120 8 143.1 143.1 NUM ct-9899 120 9 x106 x106 PROPN ct-9899 120 10 sperm sperm NOUN ct-9899 120 11 / / SYM ct-9899 120 12 male.1,2 male.1,2 NOUN ct-9899 120 13 in in ADP ct-9899 120 14 this this DET ct-9899 120 15 study study NOUN ct-9899 120 16 , , PUNCT ct-9899 120 17 a a DET ct-9899 120 18 mean mean NOUN ct-9899 120 19 of of ADP ct-9899 120 20 560 560 NUM ct-9899 120 21 x106 x106 PROPN ct-9899 120 22 sperm sperm NOUN ct-9899 120 23 / / SYM ct-9899 120 24 male male NOUN ct-9899 120 25 ( ( PUNCT ct-9899 120 26 range range NOUN ct-9899 120 27 203 203 NUM ct-9899 120 28 to to ADP ct-9899 120 29 898.5 898.5 NUM ct-9899 120 30 x106 x106 PROPN ct-9899 120 31 sperm sperm NOUN ct-9899 120 32 / / SYM ct-9899 120 33 male male NOUN ct-9899 120 34 ) ) PUNCT ct-9899 120 35 was be AUX ct-9899 120 36 obtained obtain VERB ct-9899 120 37 . . PUNCT ct-9899 121 1 pre pre ADJ ct-9899 121 2 - - ADJ ct-9899 121 3 freezing freezing ADJ ct-9899 121 4 sperm sperm NOUN ct-9899 121 5 motility motility NOUN ct-9899 121 6 was be AUX ct-9899 121 7 comparable comparable ADJ ct-9899 121 8 to to ADP ct-9899 121 9 previous previous ADJ ct-9899 121 10 reports.1,2,15 reports.1,2,15 ADV ct-9899 121 11 the the DET ct-9899 121 12 mean mean ADJ ct-9899 121 13 percentage percentage NOUN ct-9899 121 14 of of ADP ct-9899 121 15 morphologically morphologically ADV ct-9899 121 16 normal normal ADJ ct-9899 121 17 sperm sperm NOUN ct-9899 121 18 was be AUX ct-9899 121 19 29 29 NUM ct-9899 121 20 % % NOUN ct-9899 121 21 . . PUNCT ct-9899 122 1 the the DET ct-9899 122 2 main main ADJ ct-9899 122 3 abnormality abnormality NOUN ct-9899 122 4 present present NOUN ct-9899 122 5 was be AUX ct-9899 122 6 cytoplasmic cytoplasmic ADJ ct-9899 122 7 droplets droplet NOUN ct-9899 122 8 ( ( PUNCT ct-9899 122 9 45 45 NUM ct-9899 122 10 % % NOUN ct-9899 122 11 ) ) PUNCT ct-9899 122 12 . . PUNCT ct-9899 123 1 cytoplasmic cytoplasmic ADJ ct-9899 123 2 droplets droplet NOUN ct-9899 123 3 can can AUX ct-9899 123 4 be be AUX ct-9899 123 5 present present ADJ ct-9899 123 6 in in ADP ct-9899 123 7 more more ADJ ct-9899 123 8 than than ADP ct-9899 123 9 60 60 NUM ct-9899 123 10 % % NOUN ct-9899 123 11 of of ADP ct-9899 123 12 alpaca alpaca NOUN ct-9899 123 13 sperm sperm NOUN ct-9899 123 14 in in ADP ct-9899 123 15 the the DET ct-9899 123 16 terminal terminal ADJ ct-9899 123 17 segment segment NOUN ct-9899 123 18 of of ADP ct-9899 123 19 the the DET ct-9899 123 20 cauda cauda NOUN ct-9899 123 21 epididymis epididymis NOUN ct-9899 123 22 , , PUNCT ct-9899 123 23 and and CCONJ ct-9899 123 24 are be AUX ct-9899 123 25 lost lose VERB ct-9899 123 26 when when SCONJ ct-9899 123 27 sperm sperm NOUN ct-9899 123 28 reach reach VERB ct-9899 123 29 the the DET ct-9899 123 30 ductus ductus NOUN ct-9899 123 31 deferens.16 deferens.16 NOUN ct-9899 123 32 while while SCONJ ct-9899 123 33 these these DET ct-9899 123 34 findings finding NOUN ct-9899 123 35 represent represent VERB ct-9899 123 36 physiological physiological ADJ ct-9899 123 37 changes change NOUN ct-9899 123 38 that that PRON ct-9899 123 39 occur occur VERB ct-9899 123 40 during during ADP ct-9899 123 41 epididymal epididymal ADJ ct-9899 123 42 transit transit NOUN ct-9899 123 43 , , PUNCT ct-9899 123 44 the the DET ct-9899 123 45 effect effect NOUN ct-9899 123 46 of of ADP ct-9899 123 47 epididymal epididymal ADJ ct-9899 123 48 sperm sperm NOUN ct-9899 123 49 morphology morphology NOUN ct-9899 123 50 on on ADP ct-9899 123 51 alpaca alpaca NOUN ct-9899 123 52 fertility fertility NOUN ct-9899 123 53 is be AUX ct-9899 123 54 not not PART ct-9899 123 55 known know VERB ct-9899 123 56 . . PUNCT ct-9899 124 1 in in ADP ct-9899 124 2 conclusion conclusion NOUN ct-9899 124 3 , , PUNCT ct-9899 124 4 a a DET ct-9899 124 5 dmfa dmfa NOUN ct-9899 124 6 - - PUNCT ct-9899 124 7 containing contain VERB ct-9899 124 8 commercial commercial ADJ ct-9899 124 9 semen semen NOUN ct-9899 124 10 extender extender NOUN ct-9899 124 11 ( ( PUNCT ct-9899 124 12 botucrio botucrio NOUN ct-9899 124 13 ) ) PUNCT ct-9899 124 14 was be AUX ct-9899 124 15 superior superior ADJ ct-9899 124 16 to to ADP ct-9899 124 17 a a DET ct-9899 124 18 liposome liposome NOUN ct-9899 124 19 - - PUNCT ct-9899 124 20 containing contain VERB ct-9899 124 21 semen semen NOUN ct-9899 124 22 extender extender NOUN ct-9899 124 23 ( ( PUNCT ct-9899 124 24 optixcell optixcell NOUN ct-9899 124 25 ) ) PUNCT ct-9899 124 26 at at ADP ct-9899 124 27 preserving preserve VERB ct-9899 124 28 post post ADJ ct-9899 124 29 - - ADJ ct-9899 124 30 thaw thaw ADJ ct-9899 124 31 motility motility NOUN ct-9899 124 32 and and CCONJ ct-9899 124 33 membrane membrane NOUN ct-9899 124 34 integrity integrity NOUN ct-9899 124 35 of of ADP ct-9899 124 36 cryopreserved cryopreserve VERB ct-9899 124 37 alpaca alpaca NOUN ct-9899 124 38 epididymal epididymal ADJ ct-9899 124 39 sperm sperm NOUN ct-9899 124 40 . . PUNCT ct-9899 125 1 cold cold ADJ ct-9899 125 2 storage storage NOUN ct-9899 125 3 of of ADP ct-9899 125 4 epididymides epididymis NOUN ct-9899 125 5 for for ADP ct-9899 125 6 24 24 NUM ct-9899 125 7 h h NOUN ct-9899 125 8 negatively negatively ADV ct-9899 125 9 affected affect VERB ct-9899 125 10 post post ADJ ct-9899 125 11 - - ADJ ct-9899 125 12 thaw thaw ADJ ct-9899 125 13 sperm sperm NOUN ct-9899 125 14 motility motility NOUN ct-9899 125 15 and and CCONJ ct-9899 125 16 membrane membrane NOUN ct-9899 125 17 integrity integrity NOUN ct-9899 125 18 . . PUNCT ct-9899 126 1 therefore therefore ADV ct-9899 126 2 , , PUNCT ct-9899 126 3 cryopreservation cryopreservation NOUN ct-9899 126 4 with with ADP ct-9899 126 5 botucrio botucrio NOUN ct-9899 126 6 immediately immediately ADV ct-9899 126 7 after after SCONJ ct-9899 126 8 castration castration NOUN ct-9899 126 9 is be AUX ct-9899 126 10 recommended recommend VERB ct-9899 126 11 for for ADP ct-9899 126 12 best good ADJ ct-9899 126 13 preservation preservation NOUN ct-9899 126 14 of of ADP ct-9899 126 15 post post ADJ ct-9899 126 16 - - ADJ ct-9899 126 17 thaw thaw ADJ ct-9899 126 18 viability viability NOUN ct-9899 126 19 of of ADP ct-9899 126 20 alpaca alpaca NOUN ct-9899 126 21 epididymal epididymal ADJ ct-9899 126 22 sperm sperm NOUN ct-9899 126 23 . . PUNCT ct-9899 127 1 references reference NOUN ct-9899 127 2 1 1 NUM ct-9899 127 3 . . PUNCT ct-9899 127 4 morton morton PROPN ct-9899 127 5 km km PROPN ct-9899 127 6 , , PUNCT ct-9899 127 7 bathgate bathgate PROPN ct-9899 127 8 r r PROPN ct-9899 127 9 , , PUNCT ct-9899 127 10 evans evans PROPN ct-9899 127 11 g g PROPN ct-9899 127 12 , , PUNCT ct-9899 127 13 et et PROPN ct-9899 127 14 al al PROPN ct-9899 127 15 : : PUNCT ct-9899 127 16 cryopreservation cryopreservation NOUN ct-9899 127 17 of of ADP ct-9899 127 18 epididymal epididymal ADJ ct-9899 127 19 alpaca alpaca NOUN ct-9899 127 20 ( ( PUNCT ct-9899 127 21 vicugna vicugna PROPN ct-9899 127 22 pacos pacos PROPN ct-9899 127 23 ) ) PUNCT ct-9899 127 24 sperm sperm NOUN ct-9899 127 25 : : PUNCT ct-9899 127 26 a a DET ct-9899 127 27 comparison comparison NOUN ct-9899 127 28 of of ADP ct-9899 127 29 citrate- citrate- X ct-9899 127 30 , , PUNCT ct-9899 127 31 trisand trisand NOUN ct-9899 127 32 lactose lactose NOUN ct-9899 127 33 - - PUNCT ct-9899 127 34 based base VERB ct-9899 127 35 diluents diluent NOUN ct-9899 127 36 and and CCONJ ct-9899 127 37 pellets pellet NOUN ct-9899 127 38 and and CCONJ ct-9899 127 39 straws straw NOUN ct-9899 127 40 . . PUNCT ct-9899 128 1 reproduction reproduction NOUN ct-9899 128 2 , , PUNCT ct-9899 128 3 fertility fertility NOUN ct-9899 128 4 and and CCONJ ct-9899 128 5 development development NOUN ct-9899 128 6 2007;19:792 2007;19:792 PROPN ct-9899 128 7 - - SYM ct-9899 128 8 796 796 NUM ct-9899 128 9 . . NOUN ct-9899 129 1 2 2 NUM ct-9899 129 2 . . X ct-9899 129 3 morton morton PROPN ct-9899 129 4 km km PROPN ct-9899 129 5 , , PUNCT ct-9899 129 6 evans evans PROPN ct-9899 129 7 g g PROPN ct-9899 129 8 , , PUNCT ct-9899 129 9 maxwell maxwell PROPN ct-9899 129 10 wmc wmc PROPN ct-9899 129 11 : : PUNCT ct-9899 129 12 effect effect NOUN ct-9899 129 13 of of ADP ct-9899 129 14 glycerol glycerol NOUN ct-9899 129 15 concentration concentration NOUN ct-9899 129 16 , , PUNCT ct-9899 129 17 equex equex PROPN ct-9899 129 18 stm stm PROPN ct-9899 129 19 ® ® NOUN ct-9899 129 20 supplementation supplementation NOUN ct-9899 129 21 and and CCONJ ct-9899 129 22 liquid liquid ADJ ct-9899 129 23 storage storage NOUN ct-9899 129 24 prior prior ADV ct-9899 129 25 to to ADP ct-9899 129 26 freezing freeze VERB ct-9899 129 27 on on ADP ct-9899 129 28 the the DET ct-9899 129 29 motility motility NOUN ct-9899 129 30 and and CCONJ ct-9899 129 31 acrosome acrosome NOUN ct-9899 129 32 integrity integrity NOUN ct-9899 129 33 of of ADP ct-9899 129 34 frozen frozen ADJ ct-9899 129 35 - - PUNCT ct-9899 129 36 thawed thaw VERB ct-9899 129 37 epididymal epididymal ADJ ct-9899 129 38 alpaca alpaca NOUN ct-9899 129 39 ( ( PUNCT ct-9899 129 40 vicugna vicugna PROPN ct-9899 129 41 pacos pacos PROPN ct-9899 129 42 ) ) PUNCT ct-9899 129 43 sperm sperm NOUN ct-9899 129 44 . . PUNCT ct-9899 130 1 theriogenology theriogenology NOUN ct-9899 130 2 2010;74:311 2010;74:311 NOUN ct-9899 130 3 - - SYM ct-9899 130 4 316 316 NUM ct-9899 130 5 . . NOUN ct-9899 130 6 3 3 NUM ct-9899 130 7 . . X ct-9899 130 8 terreros terreros PROPN ct-9899 130 9 mc mc PROPN ct-9899 130 10 , , PUNCT ct-9899 130 11 huanca huanca PROPN ct-9899 130 12 wl wl PROPN ct-9899 130 13 , , PUNCT ct-9899 130 14 arriaga arriaga PROPN ct-9899 130 15 ic ic PROPN ct-9899 130 16 , , PUNCT ct-9899 130 17 et et PROPN ct-9899 130 18 al al PROPN ct-9899 130 19 : : PUNCT ct-9899 130 20 efecto efecto PROPN ct-9899 130 21 de de X ct-9899 130 22 tres tres X ct-9899 130 23 crioprotectores crioprotectore NOUN ct-9899 130 24 en en X ct-9899 130 25 la la PROPN ct-9899 130 26 criopreservacion criopreservacion PROPN ct-9899 130 27 de de X ct-9899 130 28 espermatozoides espermatozoide VERB ct-9899 130 29 epididimarios epididimario NOUN ct-9899 130 30 de de ADP ct-9899 130 31 alpaca alpaca NOUN ct-9899 130 32 . . PUNCT ct-9899 131 1 rev rev VERB ct-9899 131 2 inv inv PROPN ct-9899 131 3 vet vet PROPN ct-9899 131 4 peru peru PROPN ct-9899 131 5 2015;26:420 2015;26:420 PROPN ct-9899 131 6 - - SYM ct-9899 131 7 426 426 NUM ct-9899 131 8 . . NOUN ct-9899 131 9 4 4 NUM ct-9899 131 10 . . PROPN ct-9899 131 11 canorio canorio PROPN ct-9899 131 12 pariona pariona PROPN ct-9899 131 13 n n CCONJ ct-9899 131 14 , , PUNCT ct-9899 131 15 paredes parede VERB ct-9899 131 16 arnedo arnedo PROPN ct-9899 131 17 f f PROPN ct-9899 131 18 , , PUNCT ct-9899 131 19 valdivia valdivia PROPN ct-9899 131 20 cuya cuya PROPN ct-9899 131 21 m m PROPN ct-9899 131 22 : : PUNCT ct-9899 131 23 agentes agente NOUN ct-9899 131 24 crioprotectores crioprotectore VERB ct-9899 131 25 alternativos alternativos PROPN ct-9899 131 26 para para PROPN ct-9899 131 27 el el PROPN ct-9899 131 28 congelamiento congelamiento PROPN ct-9899 131 29 lento lento PROPN ct-9899 131 30 de de PROPN ct-9899 131 31 espermatozoides espermatozoide VERB ct-9899 131 32 epididimarios epididimario NOUN ct-9899 131 33 de de ADP ct-9899 131 34 alpaca alpaca NOUN ct-9899 131 35 ( ( PUNCT ct-9899 131 36 vicugna vicugna PROPN ct-9899 131 37 pacos pacos PROPN ct-9899 131 38 ) ) PUNCT ct-9899 131 39 . . PUNCT ct-9899 132 1 rev rev PROPN ct-9899 132 2 inv inv PROPN ct-9899 132 3 vet vet NOUN ct-9899 132 4 peru peru PROPN ct-9899 132 5 2015;26:434 2015;26:434 PROPN ct-9899 132 6 - - SYM ct-9899 132 7 443 443 NUM ct-9899 132 8 . . NOUN ct-9899 133 1 5 5 NUM ct-9899 133 2 . . X ct-9899 133 3 morillo morillo PROPN ct-9899 133 4 rodriguez rodriguez PROPN ct-9899 133 5 a a PROPN ct-9899 133 6 , , PUNCT ct-9899 133 7 balao balao PROPN ct-9899 133 8 da da PROPN ct-9899 133 9 silva silva PROPN ct-9899 133 10 c c PROPN ct-9899 133 11 , , PUNCT ct-9899 133 12 macıas macıas ADJ ct-9899 133 13 - - PUNCT ct-9899 133 14 garcıa garcıa ADJ ct-9899 133 15 b b NOUN ct-9899 133 16 , , PUNCT ct-9899 133 17 et et PROPN ct-9899 133 18 al al PROPN ct-9899 133 19 : : PUNCT ct-9899 133 20 dimethylformamide dimethylformamide NOUN ct-9899 133 21 improves improve VERB ct-9899 133 22 the the DET ct-9899 133 23 in in X ct-9899 133 24 vitro vitro X ct-9899 133 25 characteristics characteristic NOUN ct-9899 133 26 of of ADP ct-9899 133 27 thawed thaw VERB ct-9899 133 28 stallion stallion NOUN ct-9899 133 29 spermatozoa spermatozoon NOUN ct-9899 133 30 reducing reduce VERB ct-9899 133 31 sublethal sublethal NOUN ct-9899 133 32 damage damage NOUN ct-9899 133 33 . . PUNCT ct-9899 134 1 reprod reprod PROPN ct-9899 134 2 dom dom PROPN ct-9899 134 3 anim anim NOUN ct-9899 134 4 2012;47:995 2012;47:995 PROPN ct-9899 134 5 - - PUNCT ct-9899 134 6 1002 1002 NUM ct-9899 134 7 . . PUNCT ct-9899 135 1 6 6 NUM ct-9899 135 2 . . X ct-9899 135 3 álvarez álvarez PROPN ct-9899 135 4 c c PROPN ct-9899 135 5 , , PUNCT ct-9899 135 6 gil gil PROPN ct-9899 135 7 l l PROPN ct-9899 135 8 , , PUNCT ct-9899 135 9 gonzález gonzález PROPN ct-9899 135 10 n n CCONJ ct-9899 135 11 , , PUNCT ct-9899 135 12 et et PROPN ct-9899 135 13 al al PROPN ct-9899 135 14 : : PUNCT ct-9899 135 15 equine equine NOUN ct-9899 135 16 sperm sperm NOUN ct-9899 135 17 post post ADJ ct-9899 135 18 - - ADJ ct-9899 135 19 thaw thaw ADJ ct-9899 135 20 evaluation evaluation NOUN ct-9899 135 21 after after ADP ct-9899 135 22 the the DET ct-9899 135 23 addition addition NOUN ct-9899 135 24 of of ADP ct-9899 135 25 different different ADJ ct-9899 135 26 cryoprotectants cryoprotectant NOUN ct-9899 135 27 added add VERB ct-9899 135 28 to to ADP ct-9899 135 29 inra inra NOUN ct-9899 135 30 96 96 NUM ct-9899 135 31 extender extender NOUN ct-9899 135 32 . . PUNCT ct-9899 136 1 cryobiology cryobiology NOUN ct-9899 136 2 2014;69:144 2014;69:144 NUM ct-9899 136 3 - - SYM ct-9899 136 4 148 148 NUM ct-9899 136 5 . . PUNCT ct-9899 137 1 7 7 X ct-9899 137 2 . . X ct-9899 137 3 ansari ansari PROPN ct-9899 137 4 ms ms PROPN ct-9899 137 5 , , PUNCT ct-9899 137 6 rakha rakha PROPN ct-9899 137 7 ba ba PROPN ct-9899 137 8 , , PUNCT ct-9899 137 9 akhter akhter NOUN ct-9899 137 10 s s PROPN ct-9899 137 11 , , PUNCT ct-9899 137 12 et et PROPN ct-9899 137 13 al al PROPN ct-9899 137 14 . . PUNCT ct-9899 137 15 : : PUNCT ct-9899 138 1 optixcell optixcell INTJ ct-9899 138 2 improves improve VERB ct-9899 138 3 the the DET ct-9899 138 4 postthaw postthaw NOUN ct-9899 138 5 quality quality NOUN ct-9899 138 6 and and CCONJ ct-9899 138 7 fertility fertility NOUN ct-9899 138 8 of of ADP ct-9899 138 9 buffalo buffalo PROPN ct-9899 138 10 bull bull NOUN ct-9899 138 11 sperm sperm NOUN ct-9899 138 12 . . PUNCT ct-9899 139 1 theriogenology theriogenology NOUN ct-9899 139 2 2016;85:528 2016;85:528 X ct-9899 139 3 - - SYM ct-9899 139 4 532 532 NUM ct-9899 139 5 . . NOUN ct-9899 139 6 8 8 NUM ct-9899 139 7 . . X ct-9899 140 1 miguel miguel PROPN ct-9899 140 2 - - PUNCT ct-9899 140 3 jiménez jiménez PROPN ct-9899 140 4 s s PART ct-9899 140 5 , , PUNCT ct-9899 140 6 mogas mogas PROPN ct-9899 140 7 t t PROPN ct-9899 140 8 , , PUNCT ct-9899 140 9 peña peña PROPN ct-9899 140 10 ai ai VERB ct-9899 140 11 , , PUNCT ct-9899 140 12 et et PROPN ct-9899 140 13 al al PROPN ct-9899 140 14 : : PUNCT ct-9899 140 15 post post ADJ ct-9899 140 16 - - ADJ ct-9899 140 17 thaw thaw ADJ ct-9899 140 18 changes change NOUN ct-9899 140 19 in in ADP ct-9899 140 20 sperm sperm NOUN ct-9899 140 21 membrane membrane NOUN ct-9899 140 22 and and CCONJ ct-9899 140 23 ros ros PROPN ct-9899 140 24 following follow VERB ct-9899 140 25 cryopreservation cryopreservation NOUN ct-9899 140 26 of of ADP ct-9899 140 27 dairy dairy NOUN ct-9899 140 28 bull bull NOUN ct-9899 140 29 semen semen NOUN ct-9899 140 30 using use VERB ct-9899 140 31 four four NUM ct-9899 140 32 different different ADJ ct-9899 140 33 commercial commercial ADJ ct-9899 140 34 extenders extender NOUN ct-9899 140 35 . . PUNCT ct-9899 141 1 anim anim NOUN ct-9899 141 2 reprod reprod PROPN ct-9899 141 3 2016;13:573 2016;13:573 PROPN ct-9899 141 4 . . PUNCT ct-9899 142 1 9 9 NUM ct-9899 142 2 . . PUNCT ct-9899 143 1 james james PROPN ct-9899 143 2 an an PROPN ct-9899 143 3 , , PUNCT ct-9899 143 4 green green ADJ ct-9899 143 5 h h NOUN ct-9899 143 6 , , PUNCT ct-9899 143 7 hoffman hoffman NOUN ct-9899 143 8 s s PROPN ct-9899 143 9 , , PUNCT ct-9899 143 10 et et PROPN ct-9899 143 11 al al PROPN ct-9899 143 12 : : PUNCT ct-9899 143 13 preservation preservation NOUN ct-9899 143 14 of of ADP ct-9899 143 15 equine equine NOUN ct-9899 143 16 sperm sperm NOUN ct-9899 143 17 stored store VERB ct-9899 143 18 at at ADP ct-9899 143 19 4 4 NUM ct-9899 143 20 c c VERB ct-9899 143 21 for for ADP ct-9899 143 22 24 24 NUM ct-9899 143 23 , , PUNCT ct-9899 143 24 48 48 NUM ct-9899 143 25 , , PUNCT ct-9899 143 26 72 72 NUM ct-9899 143 27 and and CCONJ ct-9899 143 28 96 96 NUM ct-9899 143 29 hours hour NOUN ct-9899 143 30 . . PUNCT ct-9899 144 1 theriogenology theriogenology NOUN ct-9899 144 2 2002;58:401 2002;58:401 NOUN ct-9899 144 3 - - SYM ct-9899 144 4 404 404 NUM ct-9899 144 5 . . PUNCT ct-9899 145 1 10 10 NUM ct-9899 145 2 . . PUNCT ct-9899 145 3 monteiro monteiro PROPN ct-9899 145 4 ga ga PROPN ct-9899 145 5 , , PUNCT ct-9899 145 6 papa papa NOUN ct-9899 145 7 fo fo NOUN ct-9899 145 8 , , PUNCT ct-9899 145 9 zhan zhan PROPN ct-9899 145 10 fs fs PROPN ct-9899 145 11 , , PUNCT ct-9899 145 12 et et PROPN ct-9899 145 13 al al PROPN ct-9899 145 14 : : PUNCT ct-9899 145 15 cryopreservation cryopreservation NOUN ct-9899 145 16 and and CCONJ ct-9899 145 17 fertility fertility NOUN ct-9899 145 18 of of ADP ct-9899 145 19 ejaculated ejaculate VERB ct-9899 145 20 and and CCONJ ct-9899 145 21 epididymal epididymal ADJ ct-9899 145 22 stallion stallion NOUN ct-9899 145 23 sperm sperm NOUN ct-9899 145 24 . . PUNCT ct-9899 146 1 anim anim NOUN ct-9899 146 2 reprod reprod PROPN ct-9899 146 3 sci sci PROPN ct-9899 146 4 2011;127:197 2011;127:197 NUM ct-9899 146 5 - - NUM ct-9899 146 6 201 201 NUM ct-9899 146 7 . . PUNCT ct-9899 146 8 11 11 NUM ct-9899 146 9 . . PUNCT ct-9899 147 1 skidmore skidmore PROPN ct-9899 147 2 ja ja PROPN ct-9899 147 3 , , PUNCT ct-9899 147 4 morton morton PROPN ct-9899 147 5 km km PROPN ct-9899 147 6 , , PUNCT ct-9899 147 7 billah billah PROPN ct-9899 147 8 m m PROPN ct-9899 147 9 : : PUNCT ct-9899 147 10 artificial artificial ADJ ct-9899 147 11 insemination insemination NOUN ct-9899 147 12 in in ADP ct-9899 147 13 dromedary dromedary ADJ ct-9899 147 14 camels camel NOUN ct-9899 147 15 . . PUNCT ct-9899 148 1 anim anim NOUN ct-9899 148 2 reprod reprod PROPN ct-9899 148 3 sci sci PROPN ct-9899 148 4 2013;136:178186 2013;136:178186 NUM ct-9899 148 5 . . PUNCT ct-9899 149 1 12 12 NUM ct-9899 149 2 . . PUNCT ct-9899 150 1 ferrer ferrer PROPN ct-9899 150 2 ms ms PROPN ct-9899 150 3 , , PUNCT ct-9899 150 4 lyle lyle PROPN ct-9899 150 5 sk sk PROPN ct-9899 150 6 , , PUNCT ct-9899 150 7 eilts eilt NOUN ct-9899 150 8 be be AUX ct-9899 150 9 , , PUNCT ct-9899 150 10 et et PROPN ct-9899 150 11 al al PROPN ct-9899 150 12 : : PUNCT ct-9899 150 13 factors factor NOUN ct-9899 150 14 affecting affect VERB ct-9899 150 15 sperm sperm NOUN ct-9899 150 16 recovery recovery NOUN ct-9899 150 17 rates rate NOUN ct-9899 150 18 and and CCONJ ct-9899 150 19 survival survival NOUN ct-9899 150 20 after after ADP ct-9899 150 21 centrifugation centrifugation NOUN ct-9899 150 22 of of ADP ct-9899 150 23 equine equine NOUN ct-9899 150 24 semen semen NOUN ct-9899 150 25 . . PUNCT ct-9899 151 1 theriogenology theriogenology NOUN ct-9899 151 2 2012;78:1814 2012;78:1814 PROPN ct-9899 151 3 - - SYM ct-9899 151 4 1823 1823 NUM ct-9899 151 5 . . PUNCT ct-9899 152 1 13 13 NUM ct-9899 152 2 . . PUNCT ct-9899 153 1 turri turri PROPN ct-9899 153 2 f f PROPN ct-9899 153 3 , , PUNCT ct-9899 153 4 madeddu madeddu PROPN ct-9899 153 5 m m PROPN ct-9899 153 6 , , PUNCT ct-9899 153 7 gliozzi gliozzi NOUN ct-9899 153 8 tm tm PROPN ct-9899 153 9 , , PUNCT ct-9899 153 10 et et PROPN ct-9899 153 11 al al PROPN ct-9899 153 12 : : PUNCT ct-9899 153 13 effect effect NOUN ct-9899 153 14 of of ADP ct-9899 153 15 testicle testicle NOUN ct-9899 153 16 postmortem postmortem NOUN ct-9899 153 17 storage storage NOUN ct-9899 153 18 on on ADP ct-9899 153 19 goat goat PROPN ct-9899 153 20 frozen freeze VERB ct-9899 153 21 - - PUNCT ct-9899 153 22 thawed thaw VERB ct-9899 153 23 epididymal epididymal ADJ ct-9899 153 24 sperm sperm NOUN ct-9899 153 25 quality quality NOUN ct-9899 153 26 as as ADP ct-9899 153 27 a a DET ct-9899 153 28 tool tool NOUN ct-9899 153 29 to to PART ct-9899 153 30 improve improve VERB ct-9899 153 31 genebanking genebanke VERB ct-9899 153 32 in in ADP ct-9899 153 33 local local ADJ ct-9899 153 34 breeds breed NOUN ct-9899 153 35 . . PUNCT ct-9899 154 1 animal animal NOUN ct-9899 154 2 2014;8:440 2014;8:440 NOUN ct-9899 154 3 - - SYM ct-9899 154 4 447 447 NUM ct-9899 154 5 . . PUNCT ct-9899 154 6 14 14 NUM ct-9899 154 7 . . PUNCT ct-9899 155 1 tamayo tamayo NOUN ct-9899 155 2 - - PUNCT ct-9899 155 3 canul canul PROPN ct-9899 155 4 j j PROPN ct-9899 155 5 , , PUNCT ct-9899 155 6 alvarez alvarez PROPN ct-9899 155 7 m m PROPN ct-9899 155 8 , , PUNCT ct-9899 155 9 mata mata PROPN ct-9899 155 10 - - PUNCT ct-9899 155 11 campuzano campuzano PROPN ct-9899 155 12 m m PROPN ct-9899 155 13 , , PUNCT ct-9899 155 14 et et PROPN ct-9899 155 15 al al PROPN ct-9899 155 16 : : PUNCT ct-9899 155 17 effect effect NOUN ct-9899 155 18 of of ADP ct-9899 155 19 storage storage NOUN ct-9899 155 20 method method NOUN ct-9899 155 21 and and CCONJ ct-9899 155 22 extender extender NOUN ct-9899 155 23 osmolality osmolality NOUN ct-9899 155 24 in in ADP ct-9899 155 25 the the DET ct-9899 155 26 quality quality NOUN ct-9899 155 27 of of ADP ct-9899 155 28 cryopreserved cryopreserved ADJ ct-9899 155 29 epididymal epididymal ADJ ct-9899 155 30 ram ram NOUN ct-9899 155 31 spermatozoa spermatozoon NOUN ct-9899 155 32 . . PUNCT ct-9899 156 1 anim anim PROPN ct-9899 156 2 reprod reprod PROPN ct-9899 156 3 sci sci PROPN ct-9899 156 4 2011;129:188 2011;129:188 NUM ct-9899 156 5 - - SYM ct-9899 156 6 199 199 NUM ct-9899 156 7 . . PUNCT ct-9899 157 1 423 423 NUM ct-9899 157 2 clinical clinical ADJ ct-9899 157 3 theriogenology theriogenology NOUN ct-9899 157 4 • • NOUN ct-9899 157 5 volume volume NOUN ct-9899 157 6 10 10 NUM ct-9899 157 7 , , PUNCT ct-9899 157 8 number number NOUN ct-9899 157 9 4 4 NUM ct-9899 157 10 • • NOUN ct-9899 157 11 december december PROPN ct-9899 157 12 2018 2018 NUM ct-9899 157 13 15 15 NUM ct-9899 157 14 . . PUNCT ct-9899 158 1 santiani santiani PROPN ct-9899 158 2 a a PRON ct-9899 158 3 , , PUNCT ct-9899 158 4 ugarelli ugarelli NOUN ct-9899 158 5 a a NOUN ct-9899 158 6 , , PUNCT ct-9899 158 7 evangelista evangelista ADJ ct-9899 158 8 - - PUNCT ct-9899 158 9 vargas vargas PROPN ct-9899 158 10 s s PART ct-9899 158 11 : : PUNCT ct-9899 158 12 characterization characterization NOUN ct-9899 158 13 of of ADP ct-9899 158 14 functional functional ADJ ct-9899 158 15 variables variable NOUN ct-9899 158 16 in in ADP ct-9899 158 17 epididymal epididymal ADJ ct-9899 158 18 alpaca alpaca NOUN ct-9899 158 19 ( ( PUNCT ct-9899 158 20 vicugna vicugna PROPN ct-9899 158 21 pacos pacos PROPN ct-9899 158 22 ) ) PUNCT ct-9899 158 23 sperm sperm NOUN ct-9899 158 24 using use VERB ct-9899 158 25 imaging imaging NOUN ct-9899 158 26 flow flow NOUN ct-9899 158 27 cytometry cytometry NOUN ct-9899 158 28 . . PUNCT ct-9899 159 1 anim anim PROPN ct-9899 159 2 reprod reprod PROPN ct-9899 159 3 sci sci PROPN ct-9899 159 4 2016;173:49 2016;173:49 NUM ct-9899 159 5 - - SYM ct-9899 159 6 55 55 NUM ct-9899 159 7 . . PUNCT ct-9899 159 8 16 16 NUM ct-9899 159 9 . . PUNCT ct-9899 160 1 delhon delhon PROPN ct-9899 160 2 g g PROPN ct-9899 160 3 , , PUNCT ct-9899 160 4 von von PROPN ct-9899 160 5 lawzewitsch lawzewitsch VERB ct-9899 160 6 i i PRON ct-9899 160 7 : : PUNCT ct-9899 160 8 ductus ductus NOUN ct-9899 160 9 epididymidis epididymidis NOUN ct-9899 160 10 compartments compartment NOUN ct-9899 160 11 and and CCONJ ct-9899 160 12 morphology morphology NOUN ct-9899 160 13 of of ADP ct-9899 160 14 epididymal epididymal ADJ ct-9899 160 15 spermatozoa spermatozoon NOUN ct-9899 160 16 in in ADP ct-9899 160 17 llamas llamas NOUN ct-9899 160 18 . . PUNCT ct-9899 161 1 anat anat PROPN ct-9899 161 2 histol histol PROPN ct-9899 161 3 embryol embryol NOUN ct-9899 161 4 1994;23:217 1994;23:217 PROPN ct-9899 161 5 - - PUNCT ct-9899 161 6 225 225 NUM ct-9899 161 7 . . PUNCT ct-9899 161 8 table table NOUN ct-9899 161 9 1 1 NUM ct-9899 161 10 . . PUNCT ct-9899 162 1 pre pre ADJ ct-9899 162 2 - - ADJ ct-9899 162 3 freezing freezing ADJ ct-9899 162 4 parameters parameter NOUN ct-9899 162 5 of of ADP ct-9899 162 6 epididymal epididymal ADJ ct-9899 162 7 sperm sperm NOUN ct-9899 162 8 collected collect VERB ct-9899 162 9 on on ADP ct-9899 162 10 the the DET ct-9899 162 11 day day NOUN ct-9899 162 12 of of ADP ct-9899 162 13 castration castration NOUN ct-9899 162 14 or or CCONJ ct-9899 162 15 after after ADP ct-9899 162 16 24 24 NUM ct-9899 162 17 h h NOUN ct-9899 162 18 of of ADP ct-9899 162 19 cold cold ADJ ct-9899 162 20 storage storage NOUN ct-9899 162 21 . . PUNCT ct-9899 163 1 data datum NOUN ct-9899 163 2 are be AUX ct-9899 163 3 expressed express VERB ct-9899 163 4 as as ADP ct-9899 163 5 mean mean PROPN ct-9899 163 6 ± ± NUM ct-9899 163 7 sem sem PROPN ct-9899 163 8 . . PUNCT ct-9899 164 1 parameter parameter PROPN ct-9899 164 2 day day PROPN ct-9899 164 3 of of ADP ct-9899 164 4 castration castration NOUN ct-9899 164 5 24 24 NUM ct-9899 164 6 h h NOUN ct-9899 164 7 after after ADP ct-9899 164 8 castration castration NOUN ct-9899 164 9 total total ADJ ct-9899 164 10 sperm sperm NOUN ct-9899 164 11 motility motility NOUN ct-9899 164 12 ( ( PUNCT ct-9899 164 13 % % NOUN ct-9899 164 14 ) ) PUNCT ct-9899 165 1 50.16.1 50.16.1 PROPN ct-9899 165 2 34.216.1 34.216.1 NUM ct-9899 165 3 progressive progressive ADJ ct-9899 165 4 sperm sperm NOUN ct-9899 165 5 motility motility NOUN ct-9899 165 6 ( ( PUNCT ct-9899 165 7 % % INTJ ct-9899 165 8 ) ) PUNCT ct-9899 165 9 33.75.7 33.75.7 NUM ct-9899 165 10 19.56.2 19.56.2 NUM ct-9899 165 11 normal normal ADJ ct-9899 165 12 sperm sperm NOUN ct-9899 165 13 ( ( PUNCT ct-9899 165 14 % % INTJ ct-9899 165 15 ) ) PUNCT ct-9899 165 16 29.15 29.15 NUM ct-9899 165 17 29.86 29.86 NUM ct-9899 165 18 abnormal abnormal ADJ ct-9899 165 19 acrosomes acrosome NOUN ct-9899 165 20 ( ( PUNCT ct-9899 165 21 % % INTJ ct-9899 165 22 ) ) PUNCT ct-9899 165 23 0.80.5 0.80.5 NUM ct-9899 165 24 0.30.3 0.30.3 NUM ct-9899 165 25 abnormal abnormal ADJ ct-9899 165 26 heads head NOUN ct-9899 165 27 ( ( PUNCT ct-9899 165 28 % % INTJ ct-9899 165 29 ) ) PUNCT ct-9899 165 30 6.13.1 6.13.1 NUM ct-9899 165 31 72.2 72.2 NUM ct-9899 165 32 abnormal abnormal ADJ ct-9899 165 33 midpieces midpiece NOUN ct-9899 165 34 ( ( PUNCT ct-9899 165 35 % % INTJ ct-9899 165 36 ) ) PUNCT ct-9899 165 37 6.72.1 6.72.1 NUM ct-9899 165 38 3.51.1 3.51.1 NUM ct-9899 165 39 kinked kink VERB ct-9899 165 40 tails tail NOUN ct-9899 165 41 ( ( PUNCT ct-9899 165 42 % % INTJ ct-9899 165 43 ) ) PUNCT ct-9899 165 44 7.82.3 7.82.3 NUM ct-9899 165 45 8.13 8.13 NUM ct-9899 165 46 proximal proximal ADJ ct-9899 165 47 droplets droplet NOUN ct-9899 165 48 ( ( PUNCT ct-9899 165 49 % % INTJ ct-9899 165 50 ) ) PUNCT ct-9899 165 51 32.56.8 32.56.8 PROPN ct-9899 165 52 38.34 38.34 NUM ct-9899 165 53 distal distal ADJ ct-9899 165 54 droplets droplet NOUN ct-9899 165 55 ( ( PUNCT ct-9899 165 56 % % INTJ ct-9899 165 57 ) ) PUNCT ct-9899 165 58 10.82.2 10.82.2 NUM ct-9899 165 59 9.72.3 9.72.3 NUM ct-9899 165 60 coiled coil VERB ct-9899 165 61 tails tail NOUN ct-9899 165 62 ( ( PUNCT ct-9899 165 63 % % INTJ ct-9899 165 64 ) ) PUNCT ct-9899 165 65 4.42 4.42 NOUN ct-9899 165 66 2.31.2 2.31.2 NUM ct-9899 165 67 detached detach VERB ct-9899 165 68 heads head NOUN ct-9899 165 69 ( ( PUNCT ct-9899 165 70 % % INTJ ct-9899 165 71 ) ) PUNCT ct-9899 165 72 1.41.1 1.41.1 NUM ct-9899 165 73 0.80.8 0.80.8 NUM ct-9899 165 74 sperm sperm NOUN ct-9899 165 75 concentration concentration NOUN ct-9899 165 76 ( ( PUNCT ct-9899 165 77 x106 x106 PROPN ct-9899 165 78 / / SYM ct-9899 165 79 ml ml NOUN ct-9899 165 80 ) ) PUNCT ct-9899 165 81 53.610.9 53.610.9 NUM ct-9899 165 82 71.412.5 71.412.5 NUM ct-9899 165 83 sample sample NOUN ct-9899 165 84 volume volume NOUN ct-9899 165 85 ( ( PUNCT ct-9899 165 86 ml ml NOUN ct-9899 165 87 ) ) PUNCT ct-9899 165 88 6.30.3 6.30.3 NUM ct-9899 165 89 3.70.1 3.70.1 NUM ct-9899 165 90 total total NOUN ct-9899 165 91 sperm sperm NOUN ct-9899 165 92 ( ( PUNCT ct-9899 165 93 x106 x106 NUM ct-9899 165 94 ) ) PUNCT ct-9899 165 95 326.955.9 326.955.9 NUM ct-9899 165 96 271.954.2 271.954.2 NUM ct-9899 165 97 total total VERB ct-9899 165 98 progressively progressively ADV ct-9899 165 99 motile motile ADJ ct-9899 165 100 sperm sperm NOUN ct-9899 165 101 ( ( PUNCT ct-9899 165 102 x106 x106 PROPN ct-9899 165 103 ) ) PUNCT ct-9899 165 104 121.428.8 121.428.8 NOUN ct-9899 166 1 64.923.9 64.923.9 NUM ct-9899 166 2 total total ADJ ct-9899 166 3 normal normal ADJ ct-9899 166 4 motile motile NOUN ct-9899 166 5 sperm sperm NOUN ct-9899 166 6 ( ( PUNCT ct-9899 166 7 x106 x106 PROPN ct-9899 166 8 ) ) PUNCT ct-9899 166 9 28.85.8 28.85.8 PROPN ct-9899 166 10 14.14.8 14.14.8 NUM ct-9899 166 11 table table NOUN ct-9899 166 12 2 2 NUM ct-9899 166 13 . . PUNCT ct-9899 166 14 motion motion NOUN ct-9899 166 15 parameters parameter NOUN ct-9899 166 16 of of ADP ct-9899 166 17 epididymal epididymal ADJ ct-9899 166 18 alpaca alpaca NOUN ct-9899 166 19 sperm sperm NOUN ct-9899 166 20 after after ADP ct-9899 166 21 cryopreservation cryopreservation NOUN ct-9899 166 22 in in ADP ct-9899 166 23 botucrio botucrio NOUN ct-9899 166 24 or or CCONJ ct-9899 166 25 optixcell optixcell NUM ct-9899 166 26 on on ADP ct-9899 166 27 the the DET ct-9899 166 28 day day NOUN ct-9899 166 29 of of ADP ct-9899 166 30 castration castration NOUN ct-9899 166 31 ( ( PUNCT ct-9899 166 32 0 0 NUM ct-9899 166 33 ) ) PUNCT ct-9899 166 34 or or CCONJ ct-9899 166 35 after after ADP ct-9899 166 36 24 24 NUM ct-9899 166 37 h h NOUN ct-9899 166 38 of of ADP ct-9899 166 39 cold cold ADJ ct-9899 166 40 storage storage NOUN ct-9899 166 41 ( ( PUNCT ct-9899 166 42 24 24 NUM ct-9899 166 43 ) ) PUNCT ct-9899 166 44 . . PUNCT ct-9899 167 1 dcl dcl X ct-9899 168 1 = = NOUN ct-9899 168 2 curvilinear curvilinear ADJ ct-9899 168 3 distance distance NOUN ct-9899 168 4 , , PUNCT ct-9899 168 5 dap dap NOUN ct-9899 169 1 = = NOUN ct-9899 169 2 distance distance NOUN ct-9899 169 3 of of ADP ct-9899 169 4 average average ADJ ct-9899 169 5 path path NOUN ct-9899 169 6 , , PUNCT ct-9899 169 7 dsl dsl ADJ ct-9899 169 8 = = ADJ ct-9899 169 9 straight straight ADJ ct-9899 169 10 line line NOUN ct-9899 169 11 distance distance NOUN ct-9899 169 12 , , PUNCT ct-9899 169 13 vcl vcl PROPN ct-9899 169 14 = = PROPN ct-9899 169 15 curvilinear curvilinear ADJ ct-9899 169 16 velocity velocity NOUN ct-9899 169 17 , , PUNCT ct-9899 169 18 vap vap NOUN ct-9899 169 19 = = SYM ct-9899 169 20 average average ADJ ct-9899 169 21 path path NOUN ct-9899 169 22 velocity velocity NOUN ct-9899 169 23 , , PUNCT ct-9899 169 24 vsl vsl NOUN ct-9899 169 25 = = ADJ ct-9899 169 26 straight straight ADJ ct-9899 169 27 line line NOUN ct-9899 169 28 velocity velocity NOUN ct-9899 169 29 , , PUNCT ct-9899 169 30 lin lin PROPN ct-9899 169 31 = = NOUN ct-9899 169 32 linearity linearity PROPN ct-9899 169 33 , , PUNCT ct-9899 169 34 str str NOUN ct-9899 169 35 = = SYM ct-9899 169 36 straightness straightness NOUN ct-9899 169 37 , , PUNCT ct-9899 169 38 wob wob ADP ct-9899 169 39 = = NOUN ct-9899 169 40 wobble wobble ADJ ct-9899 169 41 , , PUNCT ct-9899 169 42 bcf= bcf= PROPN ct-9899 169 43 beat beat VERB ct-9899 169 44 cross cross NOUN ct-9899 169 45 frequency frequency NOUN ct-9899 169 46 , , PUNCT ct-9899 169 47 alh= alh= PROPN ct-9899 169 48 amplitude amplitude NOUN ct-9899 169 49 of of ADP ct-9899 169 50 lateral lateral ADJ ct-9899 169 51 head head NOUN ct-9899 169 52 displacement displacement NOUN ct-9899 169 53 . . PUNCT ct-9899 170 1 data datum NOUN ct-9899 170 2 are be AUX ct-9899 170 3 expressed express VERB ct-9899 170 4 as as ADP ct-9899 170 5 mean mean PROPN ct-9899 170 6 ± ± NUM ct-9899 170 7 sem sem NOUN ct-9899 170 8 . . PUNCT ct-9899 171 1 variable variable ADJ ct-9899 171 2 botucrio0 botucrio0 PROPN ct-9899 171 3 optixcell0 optixcell0 PROPN ct-9899 171 4 botucrio24 botucrio24 PROPN ct-9899 171 5 optixcell24 optixcell24 PROPN ct-9899 171 6 dcl dcl PROPN ct-9899 171 7 ( ( PUNCT ct-9899 171 8 m m PROPN ct-9899 171 9 ) ) PUNCT ct-9899 171 10 34.52 34.52 NUM ct-9899 171 11 35.84.5 35.84.5 PROPN ct-9899 171 12 34.52.4 34.52.4 NUM ct-9899 171 13 32.85.2 32.85.2 NUM ct-9899 171 14 dap dap PROPN ct-9899 171 15 ( ( PUNCT ct-9899 171 16 m m PROPN ct-9899 171 17 ) ) PUNCT ct-9899 172 1 17.90.9 17.90.9 NUM ct-9899 173 1 16.70.9 16.70.9 NUM ct-9899 173 2 16.90.5 16.90.5 NUM ct-9899 173 3 15.82.1 15.82.1 NUM ct-9899 173 4 dsl dsl ADJ ct-9899 173 5 ( ( PUNCT ct-9899 173 6 m m PROPN ct-9899 173 7 ) ) PUNCT ct-9899 173 8 10.50.4 10.50.4 NUM ct-9899 173 9 10.20.4 10.20.4 NUM ct-9899 173 10 100.4 100.4 NUM ct-9899 173 11 10.40.9 10.40.9 NUM ct-9899 173 12 vcl vcl NOUN ct-9899 173 13 ( ( PUNCT ct-9899 173 14 m m PROPN ct-9899 173 15 / / SYM ct-9899 173 16 sec sec PROPN ct-9899 173 17 ) ) PUNCT ct-9899 173 18 75.54.5 75.54.5 PROPN ct-9899 174 1 79.28.6 79.28.6 NUM ct-9899 174 2 75.64.6 75.64.6 NUM ct-9899 174 3 70.511 70.511 NUM ct-9899 174 4 vap vap NOUN ct-9899 174 5 ( ( PUNCT ct-9899 174 6 m m PROPN ct-9899 174 7 / / SYM ct-9899 174 8 sec sec PROPN ct-9899 174 9 ) ) PUNCT ct-9899 174 10 39.72 39.72 NUM ct-9899 174 11 38.21.7 38.21.7 NUM ct-9899 174 12 37.41.2 37.41.2 NUM ct-9899 174 13 34.64.7 34.64.7 PROPN ct-9899 174 14 vsl vsl X ct-9899 174 15 ( ( PUNCT ct-9899 174 16 m m PROPN ct-9899 174 17 / / SYM ct-9899 174 18 sec sec PROPN ct-9899 174 19 ) ) PUNCT ct-9899 174 20 23.51.2 23.51.2 NUM ct-9899 174 21 23.91.2 23.91.2 NUM ct-9899 174 22 22.51.1 22.51.1 NUM ct-9899 174 23 22.82.2 22.82.2 NUM ct-9899 174 24 lin lin PROPN ct-9899 174 25 0.310.01 0.310.01 NUM ct-9899 174 26 0.30.04 0.30.04 PROPN ct-9899 174 27 0.30.03 0.30.03 NOUN ct-9899 174 28 0.340.03 0.340.03 NUM ct-9899 174 29 str str NOUN ct-9899 174 30 0.590.02 0.590.02 NUM ct-9899 174 31 0.620.02 0.620.02 NUM ct-9899 175 1 0.60.03 0.60.03 NOUN ct-9899 175 2 0.670.02 0.670.02 NUM ct-9899 175 3 wob wob NOUN ct-9899 175 4 0.520.01 0.520.01 NUM ct-9899 176 1 0.50.04 0.50.04 NOUN ct-9899 176 2 0.50.03 0.50.03 NOUN ct-9899 176 3 0.510.04 0.510.04 NUM ct-9899 176 4 bcf bcf NOUN ct-9899 176 5 ( ( PUNCT ct-9899 176 6 hertz hertz NOUN ct-9899 176 7 ) ) PUNCT ct-9899 176 8 14.70.6 14.70.6 NUM ct-9899 176 9 14.91.6 14.91.6 NUM ct-9899 176 10 14.70.5 14.70.5 NUM ct-9899 176 11 12.81.8 12.81.8 NUM ct-9899 176 12 alh alh PROPN ct-9899 176 13 ( ( PUNCT ct-9899 176 14 m m PROPN ct-9899 176 15 ) ) PUNCT ct-9899 176 16 3.80.3 3.80.3 NUM ct-9899 176 17 3.90.1 3.90.1 NUM ct-9899 176 18 3.50.09 3.50.09 NUM ct-9899 176 19 3.10.4 3.10.4 NUM ct-9899 176 20 424clinical 424clinical PROPN ct-9899 176 21 theriogenology theriogenology NOUN ct-9899 176 22 • • NOUN ct-9899 176 23 volume volume NOUN ct-9899 176 24 10 10 NUM ct-9899 176 25 , , PUNCT ct-9899 176 26 number number NOUN ct-9899 176 27 4 4 NUM ct-9899 176 28 • • NOUN ct-9899 176 29 december december PROPN ct-9899 176 30 2018 2018 NUM ct-9899 176 31 figure figure NOUN ct-9899 176 32 . . PUNCT ct-9899 177 1 motility motility NOUN ct-9899 177 2 of of ADP ct-9899 177 3 alpaca alpaca NOUN ct-9899 177 4 epididymal epididymal ADJ ct-9899 177 5 sperm sperm NOUN ct-9899 177 6 prior prior ADV ct-9899 177 7 to to ADP ct-9899 177 8 freezing freeze VERB ct-9899 177 9 ( ( PUNCT ct-9899 177 10 prefreeze prefreeze NOUN ct-9899 177 11 ) ) PUNCT ct-9899 177 12 , , PUNCT ct-9899 177 13 and and CCONJ ct-9899 177 14 after after ADP ct-9899 177 15 cryopreservation cryopreservation NOUN ct-9899 177 16 in in ADP ct-9899 177 17 botucrio botucrio NOUN ct-9899 177 18 or or CCONJ ct-9899 177 19 optixcell optixcell NUM ct-9899 177 20 on on ADP ct-9899 177 21 the the DET ct-9899 177 22 day day NOUN ct-9899 177 23 of of ADP ct-9899 177 24 castration castration NOUN ct-9899 177 25 ( ( PUNCT ct-9899 177 26 0 0 NUM ct-9899 177 27 ) ) PUNCT ct-9899 177 28 or or CCONJ ct-9899 177 29 after after ADP ct-9899 177 30 24 24 NUM ct-9899 177 31 h h NOUN ct-9899 177 32 of of ADP ct-9899 177 33 cold cold ADJ ct-9899 177 34 storage storage NOUN ct-9899 177 35 ( ( PUNCT ct-9899 177 36 24 24 NUM ct-9899 177 37 ) ) PUNCT ct-9899 177 38 . . PUNCT ct-9899 178 1 membrane membrane NOUN ct-9899 178 2 integrity integrity NOUN ct-9899 178 3 was be AUX ct-9899 178 4 only only ADV ct-9899 178 5 evaluated evaluate VERB ct-9899 178 6 after after ADP ct-9899 178 7 cryopreservation cryopreservation NOUN ct-9899 178 8 . . PUNCT ct-9899 179 1 data datum NOUN ct-9899 179 2 are be AUX ct-9899 179 3 expressed express VERB ct-9899 179 4 as as ADP ct-9899 179 5 mean mean PROPN ct-9899 179 6 ± ± NUM ct-9899 179 7 sem sem NOUN ct-9899 179 8 . . PUNCT ct-9899 180 1 a a PRON ct-9899 180 2 , , PUNCT ct-9899 180 3 bindicate bindicate NOUN ct-9899 180 4 differences difference NOUN ct-9899 180 5 among among ADP ct-9899 180 6 treatments treatment NOUN ct-9899 180 7 ( ( PUNCT ct-9899 180 8 p p X ct-9899 180 9 < < X ct-9899 180 10 0.05 0.05 NUM ct-9899 180 11 ) ) PUNCT ct-9899 180 12 . . PUNCT ct-9899 181 1 425 425 NUM ct-9899 181 2 clinical clinical ADJ ct-9899 181 3 theriogenology theriogenology NOUN ct-9899 181 4 • • NOUN ct-9899 181 5 volume volume NOUN ct-9899 181 6 10 10 NUM ct-9899 181 7 , , PUNCT ct-9899 181 8 number number NOUN ct-9899 181 9 4 4 NUM ct-9899 181 10 • • NOUN ct-9899 181 11 december december PROPN ct-9899 181 12 2018 2018 NUM ct-9899 181 13 000_frontal 000_frontal ADJ ct-9899 181 14 ms_1 ms_1 NOUN ct-9899 181 15 forematter forematter NOUN ct-9899 181 16 for for ADP ct-9899 181 17 december december PROPN ct-9899 181 18 2018 2018 NUM ct-9899 181 19 ms_2 ms_2 NOUN ct-9899 181 20 letter1 letter1 NOUN ct-9899 181 21 for for ADP ct-9899 181 22 december december PROPN ct-9899 181 23 2018 2018 NUM ct-9899 181 24 ms_2 ms_2 NOUN ct-9899 181 25 letter2 letter2 X ct-9899 181 26 for for ADP ct-9899 181 27 december december PROPN ct-9899 181 28 2018 2018 NUM ct-9899 181 29 ms_2 ms_2 NOUN ct-9899 181 30 letter3 letter3 NOUN ct-9899 181 31 for for ADP ct-9899 181 32 december december PROPN ct-9899 181 33 2018 2018 NUM ct-9899 181 34 ms_3 ms_3 PROPN ct-9899 181 35 table table NOUN ct-9899 181 36 of of ADP ct-9899 181 37 contents content NOUN ct-9899 181 38 ms_4 ms_4 NOUN ct-9899 181 39 therio therio NOUN ct-9899 181 40 news news NOUN ct-9899 181 41 for for ADP ct-9899 181 42 december december PROPN ct-9899 181 43 2018_bw 2018_bw NUM ct-9899 181 44 2019 2019 NUM ct-9899 181 45 therio therio NOUN ct-9899 181 46 preview preview PROPN ct-9899 181 47 therio therio PROPN ct-9899 181 48 recap recap NOUN ct-9899 181 49 with with ADP ct-9899 181 50 pics pic NOUN ct-9899 181 51 steiner steiner NOUN ct-9899 181 52 - - PUNCT ct-9899 181 53 new new ADJ ct-9899 181 54 officers officer NOUN ct-9899 181 55 2018 2018 NUM ct-9899 181 56 therio therio NOUN ct-9899 181 57 sponsors sponsor VERB ct-9899 181 58 2018 2018 NUM ct-9899 181 59 exhibitors exhibitor NOUN ct-9899 181 60 - - PUNCT ct-9899 181 61 sft sft NOUN ct-9899 181 62 board board NOUN ct-9899 181 63 noms nom NOUN ct-9899 181 64 2018 2018 NUM ct-9899 181 65 auction auction NOUN ct-9899 181 66 donations donation NOUN ct-9899 181 67 2019 2019 NUM ct-9899 181 68 bartlett bartlett PROPN ct-9899 181 69 nomination nomination PROPN ct-9899 181 70 act act NOUN ct-9899 181 71 call call VERB ct-9899 181 72 for for ADP ct-9899 181 73 noms nom NOUN ct-9899 181 74 call call VERB ct-9899 181 75 for for ADP ct-9899 181 76 abstracts abstract NOUN ct-9899 181 77 2019 2019 NUM ct-9899 181 78 for for ADP ct-9899 181 79 ctj ctj NOUN ct-9899 181 80 the the DET ct-9899 181 81 society society NOUN ct-9899 181 82 for for ADP ct-9899 181 83 theriogenology theriogenology NOUN ct-9899 181 84 and and CCONJ ct-9899 181 85 american american PROPN ct-9899 181 86 college college PROPN ct-9899 181 87 of of ADP ct-9899 181 88 theriogenologists theriogenologist NOUN ct-9899 181 89 issue issue VERB ct-9899 181 90 a a DET ct-9899 181 91 call call NOUN ct-9899 181 92 for for ADP ct-9899 181 93 research research NOUN ct-9899 181 94 and and CCONJ ct-9899 181 95 case case NOUN ct-9899 181 96 abstracts abstract VERB ct-9899 181 97 to to PART ct-9899 181 98 be be AUX ct-9899 181 99 presented present VERB ct-9899 181 100 at at ADP ct-9899 181 101 the the DET ct-9899 181 102 annual annual ADJ ct-9899 181 103 therio therio NOUN ct-9899 181 104 conference conference PROPN ct-9899 181 105 july july PROPN ct-9899 181 106 24 24 NUM ct-9899 181 107 - - SYM ct-9899 181 108 27 27 NUM ct-9899 181 109 , , PUNCT ct-9899 181 110 2019 2019 NUM ct-9899 181 111 in in ADP ct-9899 181 112 savannah savannah PROPN ct-9899 181 113 , , PUNCT ct-9899 181 114 georgia georgia PROPN ct-9899 181 115 . . PUNCT ct-9899 182 1 1 1 X ct-9899 182 2 . . X ct-9899 182 3 competitive competitive ADJ ct-9899 182 4 category category NOUN ct-9899 182 5 : : PUNCT ct-9899 182 6 2 2 X ct-9899 182 7 . . NUM ct-9899 182 8 non‐competitive non‐competitive ADJ ct-9899 182 9 category category NOUN ct-9899 182 10 : : PUNCT ct-9899 182 11 3 3 X ct-9899 182 12 . . X ct-9899 182 13 case case NOUN ct-9899 182 14 abstract abstract ADJ ct-9899 182 15 category category NOUN ct-9899 182 16 : : PUNCT ct-9899 183 1 4 4 X ct-9899 183 2 . . X ct-9899 183 3 poster poster NOUN ct-9899 183 4 category category NOUN ct-9899 183 5 : : PUNCT ct-9899 183 6 general general ADJ ct-9899 183 7 : : PUNCT ct-9899 183 8 guidelines guideline NOUN ct-9899 183 9 : : PUNCT ct-9899 183 10 what what PRON ct-9899 183 11 to to PART ct-9899 183 12 send send VERB ct-9899 183 13 and and CCONJ ct-9899 183 14 how how SCONJ ct-9899 183 15 to to PART ct-9899 183 16 send send VERB ct-9899 183 17 it it PRON ct-9899 183 18 abstracts abstract VERB ct-9899 183 19 not not PART ct-9899 183 20 adhering adhere VERB ct-9899 183 21 to to ADP ct-9899 183 22 these these DET ct-9899 183 23 guidelines guideline NOUN ct-9899 183 24 will will AUX ct-9899 183 25 not not PART ct-9899 183 26 be be AUX ct-9899 183 27 considered consider VERB ct-9899 183 28 for for ADP ct-9899 183 29 presentation presentation NOUN ct-9899 183 30 . . PUNCT ct-9899 184 1 deadline deadline NOUN ct-9899 184 2 : : PUNCT ct-9899 184 3 abstracts abstract NOUN ct-9899 184 4 must must AUX ct-9899 184 5 be be AUX ct-9899 184 6 received receive VERB ct-9899 184 7 no no ADV ct-9899 184 8 later later ADV ct-9899 184 9 than than ADP ct-9899 184 10 february february PROPN ct-9899 184 11 15 15 NUM ct-9899 184 12 , , PUNCT ct-9899 184 13 2019 2019 NUM ct-9899 184 14 for for ADP ct-9899 184 15 consideration consideration NOUN ct-9899 184 16 . . PUNCT ct-9899 185 1 call call VERB ct-9899 185 2 for for ADP ct-9899 185 3 2019 2019 NUM ct-9899 185 4 student student NOUN ct-9899 185 5 case case NOUN ct-9899 185 6 presentations.pdf presentations.pdf NOUN ct-9899 185 7 call call VERB ct-9899 185 8 for for ADP ct-9899 185 9 student student NOUN ct-9899 185 10 case case NOUN ct-9899 185 11 presentations-2019 presentations-2019 PRON ct-9899 185 12 annual annual ADJ ct-9899 185 13 therio therio NOUN ct-9899 185 14 conference conference NOUN ct-9899 185 15 student student NOUN ct-9899 185 16 case case NOUN ct-9899 185 17 presentations presentation NOUN ct-9899 185 18 call call VERB ct-9899 185 19 for for ADP ct-9899 185 20 student student NOUN ct-9899 185 21 case case NOUN ct-9899 185 22 presentations presentation NOUN ct-9899 185 23 also also ADV ct-9899 185 24 available available ADJ ct-9899 185 25 at at ADP ct-9899 185 26 www.therio.org www.therio.org PROPN ct-9899 185 27 guidelines guideline NOUN ct-9899 185 28 for for ADP ct-9899 185 29 application application NOUN ct-9899 185 30 submission submission NOUN ct-9899 185 31 society society NOUN ct-9899 185 32 for for ADP ct-9899 185 33 theriogenology theriogenology NOUN ct-9899 185 34 student student NOUN ct-9899 185 35 case case NOUN ct-9899 185 36 presentation presentation NOUN ct-9899 185 37 competition competition NOUN ct-9899 185 38 annual annual ADJ ct-9899 185 39 therio therio NOUN ct-9899 185 40 conference conference PROPN ct-9899 185 41 , , PUNCT ct-9899 185 42 savannah savannah PROPN ct-9899 185 43 , , PUNCT ct-9899 185 44 georgia georgia PROPN ct-9899 185 45 , , PUNCT ct-9899 185 46 july july PROPN ct-9899 185 47 24 24 NUM ct-9899 185 48 - - SYM ct-9899 185 49 27 27 NUM ct-9899 185 50 , , PUNCT ct-9899 185 51 2019 2019 NUM ct-9899 185 52 ms_5 ms_5 PROPN ct-9899 185 53 editorial editorial NOUN ct-9899 185 54 001a_ms_6 001a_ms_6 NUM ct-9899 186 1 roberts roberts PROPN ct-9899 186 2 color color NOUN ct-9899 186 3 pictures picture NOUN ct-9899 186 4 002_ms_7 002_ms_7 PUNCT ct-9899 187 1 root root NOUN ct-9899 187 2 kustritz kustritz PROPN ct-9899 187 3 003_ms_8 003_ms_8 PROPN ct-9899 188 1 ferrer ferrer PROPN ct-9899 188 2 004_ms_9 004_ms_9 PROPN ct-9899 188 3 wiley wiley PROPN ct-9899 188 4 005_ms_10 005_ms_10 PROPN ct-9899 188 5 wallace wallace PROPN ct-9899 188 6 006_ms_11 006_ms_11 NUM ct-9899 188 7 peterson peterson NOUN ct-9899 188 8 007_ms_12 007_ms_12 PROPN ct-9899 188 9 nobre nobre PROPN ct-9899 188 10 008_ms_13 008_ms_13 PROPN ct-9899 188 11 sidelinger sidelinger NOUN ct-9899 188 12 009_ms_14 009_ms_14 NOUN ct-9899 188 13 mirando mirando NOUN