1 CONTACT Michelle Kutzler Michelle.Kutzler@oregonstate.edu © 2024 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommerical 4.0 International License (http:// creativecommons.org/licenses/by-nc/4.0/), permitting all noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Citation: Clinical Theriogenology 2024, 16, 10926, http://dx.doi.org/10.58292/CT.v16.10926 Research Report Serum testosterone concentrations in alpacas and llamas after human chorionic gonadotropin stimulation before and after castration Reagan Haislip,a Anna McAllister,b Michelle Kutzlerc aDepartment of Fisheries, Wildlife, and Conservation Sciences, College of Agricultural Sciences bCarlson College of Veterinary Medicine cDepartment of Animal Rangeland Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR, USA Abstract Veterinarians perform testosterone stimulation tests in animals suspected of bilateral cryptorchidism and/or incomplete castration. Period for circulating testosterone to become undetectable after castration varies among species. To our knowledge, clearance of circulating testosterone in camelids after castration has not been examined. Testosterone stimulation with human chorionic gonad- otropin (hCG) has been described in several species, including alpacas. However, there is limited information regarding responses to hCG in male llamas. Purpose was to measure serum testosterone concentrations [T] in llamas and alpacas after hCG stimulation before and after castration. We hypothesized that [T] significantly increases after hCG treatment and decreases rapidly after castra- tion. Testosterone stimulation test (using hCG) was performed in mature male alpacas (n = 9) and llamas (n = 7) 1 day prior to and 1 day after castration. Blood samples were collected; 1 sample before and 3 samples (2, 4, and 8 hours) after hCG treatment. In llamas and alpacas [T] increased (p < 0.05) > 2 fold (from prehCG values) within 2 hours after hCG treatment. PrehCG [T] were higher (p < 0.05) in alpacas than llamas but did not differ significantly between them after hCG stimulation; 24 hours after castra- tion [T] were < 0.05 ng/ml. Keywords: Luteinizing hormone, radioimmunoassay, testes, testicular weight Introduction Veterinarians measure baseline testosterone concentrations and perform testosterone stimulation tests in male animals suspected of bilateral cryptorchidism and/or incomplete cas- tration.1 The interval for circulating testosterone to decrease to undetectable concentrations varies among species after castra- tion. In horses, testosterone half-life is 1 hour; concentrations are similar to geldings at 24 hours after castration.2 In cats, serum testosterone concentrations [T] decreased to 3-12% of precastration concentrations within 1 hour after castration; similar to castrated cats at 24 hours.3 In rats, [T] decreased significantly at 12 hours after castration; however, were detect- able until 72 hours after castration.4 To our knowledge, in camelids, clearance of circulating testosterone after castration has not been reported. Measuring baseline concentrations and changes to [T] after exogenous gonadotropin releasing hormone (GnRH) or a GnRH analogue (e.g. fertirelin acetate) treatment (induces secretion of endogenous luteinizing hormone [LH])5 is diag- nostically useful. Binding of LH to receptors in testicular inter- stitial (Leydig) cells stimulates endogenous testosterone secretion.6 Another method is human chorionic gonadotro- pin (hCG) treatment and hCG binds directly to testicular LH receptors. Testicular interstitial cells respond to intravenous hCG treatment with immediate cyclic adenosine monophos- phate (cAMP) production and rapid increase in testosterone secretion.7 Testosterone stimulation with hCG has been described in several species (Table 1). However, information (response to testosterone stimulation with hCG) in llamas is limited to small sample size (intact: n =1; cryptorchid: n = 1; castrated: n = 1).8 Purpose was to measure [T] in llamas and alpacas after hCG stimulation before and after castration. We hypothesized that [T] significantly increases after hCG treatment and is undetect- able within 24 hours after castration, and has diagnostic value mailto:Michelle.Kutzler@oregonstate.edu http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ http://dx.doi.org/10.58292/CT.v16.10926 2 Citation: Clinical Theriogenology 2024, 16, 10926, http://dx.doi.org/10.58292/CT.v16.10926 for detecting retained testicular tissue after castration or cryptorchidism. Materials and methods Mature (6.1 ± 2.9 years; range: 2-10 years) intact male alpacas (n  =  9) and llamas (n  =  7) were used. Animal experiments were approved by Oregon State University Institutional Animal Care and Use Committee (Protocol #3293). One day prior to and 1 day after castration, testosterone stimulation test (using hCG) was performed. Jugular blood samples were collected prior to intravenous treatment of hCG (2,500 IU [alpaca], 5,000 IU [llama], Chorulon®, Intervet, Inc./Merck Animal Health, Rahway, NJ) and 2, 4, and 8 hours after hCG treatment. Llama hCG dose was based on a dose used for testosterone stimulation test in a cryptorchid llama.9 Alpaca hCG dose was half of llama dose (alpaca body weight [70.8 ± 12.0 kg; range: 50.0-88.6 kg] was about half of llama body weight [152.6 ± 30.4 kg; range: 104.5-195.4 kg]) and also was similar to a dose used.10 Next day, males were castrated under intravenous general anesthesia (Telazol®, Zoetis, Parsippany, NJ) using closed cas- tration technique. Scrotal incision was made over each testis and fascia surrounding testis was stripped; spermatic cord was crushed using a modified White’s emasculator. Vaginal tunic and epididymides were removed (Figure 1) and testes were weighed. Skin incisions were allowed to heal by second intention. Cornell University Animal Health and Diagnostic Center was used to measure [T] utilizing a double antibody radioimmunoassay (Diagnostic Products Corporation, Los Angeles, CA). Samples were tested within 1 assay and intra-assay coefficients of variability were < 10%. Assay sensi- tivity was 0.1 ng/ml and the lowest detectable concentration was 0.05 ng/ml. Changes in mean ± SEM [T] after hCG treatment were ana- lyzed using repeated measures analysis of variance and Dunnett’s post hoc analysis (GraphPad Prism, San Diego, CA) and compared between species using Student’s t-test (Microsoft Excel, Redmond, WA). Simple linear regression was used to compare [T] to testicular weight (Microsoft Excel; GraphPad Prism). Data are presented as mean ± SEM. Significance was defined as p < 0.05. Results Testosterone responses to hCG treatment are summarized (Table 2) and illustrated (Figure 2). In llamas and alpacas, [T] increased (p < 0.05) > 2 fold from prehCG values within 2 hours after hCG treatment and remained elevated until 8 hours. There were no significant differences in [T] among 2, 4, and 8 hours after hCG treatment. PrehCG [T] were higher (p < 0.05) in alpacas than llamas but did not differ signifi- cantly after hCG stimulation; 24 hours after castration [T] was  <  0.05 ng/ml. Stimulation with hCG also resulted in Table 1. Review of testosterone stimulation results after hCG treatment in mammals Species Dose Results Alpaca 3,000 IU [T] increased 2-4 fold 2 hours after IV hCG injection10 Cat 500 IU [T] increased 7-5 fold in 0.5 and 2 hours after IV hCG treatment, respectively18 Cow 10,000 IU [T] increased 4-5 fold in 5 days after IM hCG treatment19 Dog 67.56 IU/kg [T] increased 2-3 fold in 0.5 and 2 hours after SC hCG treatment, respectively20 Dog 35 IU/kg [T] increased 2 fold in 6 hours after IM hCG treatment, respectively21 Dog 150 IU [T] increased 2 fold in 3 hours after IM hCG treatment22 Dog 25 IU/kg [T] increased 2 fold in 1 hour after hCG SC treatment23 Horse 5,000 IU [T] increased 2-5 fold in 1 and 12 hours after IV hCG treatment, respectively2 Horse 10,000 IU [T] increased 2-4 fold in 1 and 24 hours after IV hCG treatment, respectively1 Pig 3,000 IU [T] increased 10-20 fold in 1 and 28 hours after IV hCG treatment, respectively24 Rat 25 IU/kg [T] increased 7 fold in 1 hour after hCG treatment23 Sheep 1,000 IU [T] increased 2-8 fold in 0.5 and 3 hours after IV hCG treatment, respectively25 IU: international units; IM: intramuscular; IV: intravenous; SC: subcutaneous; [T]: serum testosterone concentrations Figure 1. Pair of alpaca testes (after vaginal tunic and epididy- mides removal) prepared for weighing http://dx.doi.org/10.58292/CT.v16.10926 Citation: Clinical Theriogenology 2024, 16, 10926, http://dx.doi.org/10.58292/CT.v16.10926 3 [T] < 0.05 ng/ml at 2, 4, and 8 hours in llamas and alpacas after castration (Table 3). PrehCG [T] were higher (p < 0.05) in alpacas than llamas but did not differ significantly after hCG stimulation; 24 hours after castration [T] was < 0.05 ng/ml. Relationships between testicular weight and [T] are provided (Figure 3). There was moderate relationship (R2 = 0.69, p = 0.02) between testicu- lar weight and [T] prior to hCG treatment in llamas, but not in alpacas (R2 = 0.13, p = 0.35). There was a weak relation- ship between testicular weight and [T] 2 hours after hCG treatment in alpacas (R2 = 0.46, p = 0.04) but not in llamas (R2  =  0.33, p  =  0.17). There was no relationship between testicular weight and [T] at 4 hours (llamas: R2  =  0.12, p = 0.44; alpacas: R2 = 0.19, p = 0.24) and 8 hours (llamas: R2 = 0.12, p = 0.45; alpacas: R2 = 0.25, p = 0.17) after hCG treatment. Discussion We demonstrated that testosterone stimulation test is a diag- nostic tool to detect testicular tissue in llamas and alpacas and documented that within 24 hours after testicular tissue removal, testosterone concentrations were undetectable; sim- ilar to horses and cats.2,3 However, anecdotal evidence (https://open.lib.umn.edu/largeanimalsurgery/chapter/ camelid-castration/) in noncamelid species suggested that males may be fertile and exhibit reproductive behaviors up to 6 weeks after castration; these parameters were not evaluated in the present study. Endogenous testosterone secretion can be stimulated by GnRH or hCG; GnRH stimulates endogenous luteinizing hormone release from anterior pituitary that stimulates testosterone secretion from testicular Leydig cells, whereas hCG directly stimulates testosterone release from Leydig cells. For this rea- son, hCG is recommended over GnRH for evaluating testicular function.11 In horses, [T] increased nearly 5 fold within 1 hour after hCG treatment.2 In alpacas [T] increased 2-4 fold at 2 hours after hCG treatment.10 Similar increases in [T] were not observed in our study in llamas and alpacas at 2, 4, and 8 hours after hCG treatment. However, after castration, hCG treatment did not increase [T] in llamas or alpacas, as in humans.12 Increased testicular weight may be correlated with increased steroidogenic cells (e.g. Leydig cells); larger mass of ste- roidogenic tissue increased Leydig cell sensitivity and resulted in higher [T].13 This was supported by a study10 in alpacas that demonstrated association (R2 = 0.72; p < 0.001) between tes- ticular weight and [T] 2 hours after hCG treatment. However, in stallions, there was no relationship between testicular weight and [T] before or after hCG treatment.14 In our study, there was only moderate (significant) relationship (R2 = 0.69; p = 0.02) between testicular weight and [T] prior to hCG treat- ment in llamas, but not in alpacas (R2 = 0.13). There was also moderate (significant) relationship (R2  =  0.46; p  =  0.04) Table 2. Testicular weights (TW) and serum testosterone concentrations ([T]) relative to the timing of human chorionic gonado- tropin (hCG) treatment in alpacas and llamas, minimum (in green font) and maximum (in red font) values are identified in each column for alpacas and llamas; *differed (p < 0.05) from prehCG value Alpaca TW PrehCG [T] 2 hours posthCG [T] 4 hours posthCG [T] 8 hours posthCG [T] 1 23.5 grams 3.23 ng/ml 10.32 ng/ml 15.53 ng/ml 20.37 ng/ml 2 22.6 grams 7.72 ng/ml 14.30 ng/ml 14.21 ng/ml 18.58 ng/ml 3 22.0 grams 3.62 ng/ml 5.98 ng/ml 10.43 ng/ml 8.53 ng/ml 4 28.8 grams 4.22 ng/ml 9.89 ng/ml 9.74 ng/ml 13.25 ng/ml 5 28.1 grams 4.44 ng/ml 7.15 ng/ml 6.33 ng/ml 6.96 ng/ml 6 17.9 grams 7.14 ng/ml 10.65 ng/ml 16.21 ng/ml 19.23 ng/ml 7 14.7 grams 0.67 ng/ml 3.81 ng/ml 6.26 ng/ml 4.71 ng/ml 8 28.9 grams 9.12 ng/ml 17.66 ng/ml 20.89 ng/ml 24.91 ng/ml 9 36.2 grams 5.60 ng/ml 18.48 ng/ml 19.83 ng/ml 24.94 ng/ml Mean ± SEM 24.7 ± 1.3 grams 5.1 ± 0.9 ng/ml 10.9 ± 1.7 ng/ml* 13.3 ± 1.8 ng/ml* 14.6 ± 2.5 ng/ml Llama TW PrehCG [T] 2 hours posthCG [T] 4 hours posthCG [T] 8 hours posthCG [T] 10 35.9 grams 1.5 ng/ml 5.0 ng/ml 5.3 ng/ml 5.71 ng/ml 11 30.1 grams 0.9 ng/ml 6.9 ng/ml 8.5 ng/ml 9.73 ng/ml 12 38.5 grams 2.1 ng/ml 10.1 ng/ml 12.3 ng/ml 15.66 ng/ml 13 32.7 grams 0.6 ng/ml 5.1 ng/ml 6.1 ng/ml 7.51 ng/ml 14 22.0 grams 0.5 ng/ml 6.1 ng/ml 9.4 ng/ml 10.42 ng/ml 15 42.0 grams 3.0 ng/ml 5.5 ng/ml 5.6 ng/ml 5.62 ng/ml 16 9.9 grams 0.2 ng/ml 2.7 ng/ml 3.3 ng/ml 3.58 ng/ml Mean ± SEM 30.2 ± 1.8 grams 1.3 ± 0.4 ng/ml 5.9 ± 0.8 ng/ml* 7.2 ± 1.1 ng/ml* 8.7 ± 1.5 ng/ml http://dx.doi.org/10.58292/CT.v16.10926 https://open.lib.umn.edu/largeanimalsurgery/chapter/camelid-castration/ https://open.lib.umn.edu/largeanimalsurgery/chapter/camelid-castration/ 4 Citation: Clinical Theriogenology 2024, 16, 10926, http://dx.doi.org/10.58292/CT.v16.10926 Table 3. Serum testosterone concentrations ([T]) relative to human chorionic gonadotropin (hCG) treatment in alpacas and lla- mas 1 day after castration; lowest limit of detection for the testosterone assay was 0.05 ng/ml Alpaca PrehCG [T] 2 hours posthCG [T] 4 hours posthCG [T] 8 hours posthCG [T] 1 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 2 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 3 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 4 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 5 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 6 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 7 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 8 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 9 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml Llama PrehCG [T] 2 hours posthCG [T] 4 hours posthCG [T] 8 hours posthCG [T] 10 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 11 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 12 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 13 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 14 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 15 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml 16 < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml < 0.05 ng/ml Figure 2. Serum testosterone concentrations in alpacas (A) and llamas (B) before and 2, 4, and 8 hours after human chorionic gonadotropin (hCG) treatment http://dx.doi.org/10.58292/CT.v16.10926 Citation: Clinical Theriogenology 2024, 16, 10926, http://dx.doi.org/10.58292/CT.v16.10926 5 between testicular weight and [T] 2 hours after hCG in alpacas but not in llamas (R2 = 0.33; p = 0.17). There was no signifi- cant relationship between testicular weight and [T] at 4 and 8 hours after hCG treatment in llamas and alpacas (R2 = 0.12- 0.25). These findings suggested that Leydig cell responsiveness to hCG is not related to testicular weight. A potential limitation of the current study was that antiMülle- rian hormone (AMH) concentrations were not measured. A recent paper15 stressed the importance of examining AMH concentrations in male alpacas. In addition to measuring tes- tosterone concentration after hCG stimulation, AMH concen- trations can provide evidence for cryptorchid testis. However, testosterone stimulation with hCG remains the gold standard for determining potential cases of cryptorchidism.16,17 Conclusion In summary, hCG treatment in mature intact male llamas and alpacas increased [T] by 2 fold within 2 hours and remained Figure 3. Relationships between serum testicular concentrations and testicular weight in alpacas (A) and llamas (B) before and 2, 4, and 8 hours after human chorionic gonadotropin (hCG) treatment. Only relationships before hCG treatment in llamas and 2 hours after hCG in alpacas were different (p < 0.05) http://dx.doi.org/10.58292/CT.v16.10926 6 Citation: Clinical Theriogenology 2024, 16, 10926, http://dx.doi.org/10.58292/CT.v16.10926 elevated for at least 8 hours. In addition, [T] was below detect- able limits within 24 hours after castration. Acknowledgements Authors thank Bernadette Stang and Dr. Tessa Fiamengo for their technical support. Funding Morris Animal Foundation and Willamette Valley Llama Foundation. 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