1 CONTACT Ramanathan Kasimanickam ramkasi@wsu.edu © 2025 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http:// creativecommons.org/licenses/by-nc/4.0/), permitting all noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 Review Report Laparoscopic artificial insemination in sheep: review and cost benefit analysis Andrew Myers, Ramanathan Kasimanickam College of Veterinary Medicine, Washington State University, Pullman, WA, USA Abstract Artificial insemination in sheep has vastly increased in popularity as preserved semen is readily available. Laparoscopic artificial insemination (LAI), a surgical procedure, involves deposition of semen directly into the uterus using a laparoscope. We have reviewed LAI, compared benefits to natural breeding and transcervical artificial insemination, described procurement, and pro- vided a cost benefit analysis. LAI is an advanced assisted reproductive technique with several benefits, including higher pregnancy rates requiring fewer sperm per breeding than vaginal or transcervical artificial inseminations. Although LAI involves a higher start-up cost compared to other techniques, it is an economically feasible option. With the continual development of new equip- ment, techniques, and semen preservation technologies, LAI can enhance herd genetics without investment in expensive genetically superior studs and maintaining them. LAI with an appropriate estrus synchronization protocol results in higher numbers of supe- rior quality lambs per ewe for higher profit margin and higher lambing rates for producers, making LAI a profitable service for a veterinary practice. Keywords: Sheep, artificial insemination, laparoscopy, fertility, economic analysis Introduction Artificial insemination (AI) technologies have dramatically increased the ability of producers to shift the genetics of their herd to meet milk and meat market demands.1 Development of AI and semen preservation has allowed producers to access top-tier genetics from around the world.2 In the small rumi- nant industry, AI is a high-impact assisted reproductive tech- nology. It supports genetic improvement and contributes to conservation of genetic resources. In addition, it is a safe and effective method of reproduction while contributing to ani- mal health by controlling the spread of several infectious diseases. The successful implementation of AI depends on effective ani- mal management, semen evaluation and preservation, estrous cycle control, estrus detection, and appropriate insemination methods. Although the use of AI dates to the 1700’s, it was not heavily researched or used until the 1900’s.3 Now, AI is a wide- spread practice and is used throughout the world. Many vari- ations and methods of AI have been developed, with species responding differently to each type of approach. Laparoscopy artificial insemination (LAI) was first reported in the 1980’s.4,5 In ewes, unlike cows, the major limiting factor is the difficulty to pass the insemination pipette through the cer- vix for intrauterine semen deposition. Ewe’s cervix is small, narrow, rigid, and tortuous (Figure 1)6 and it is relatively more difficult to navigate the inseminating pipette without transrec- tal cervical manipulation. For this reason, semen is usually deposited at cervical entrance or in the cervix (intracervical) resulting in poor pregnancy rates. Not only cervical navigation is bypassed in LAI, it also results in higher pregnancy rates compared to vaginal artificial insemination (VAI) and trans- cervical artificial insemination (TCAI).7,8 For this reason, fixed time intrauterine insemination is performed via laparoscopy; commonly used in large-scale breeding programs worldwide. Although LAI is a minimally invasive procedure it requires veterinary expertise and raises animal welfare concerns and demands specialized equipment and more labor compared to cervical insemination. Although several alternative approaches have been proposed to replace laparoscopy, LAI technique continues to be the gold standard when higher pregnancy rates are required. In small ruminants, there are 3 types of AI techniques: vaginal (pericervical), cervical (intracervical) or intrauterine (transcervical and laparoscopic technique) semen deposition.7 Objectives were to: 1. review LAI literature; 2. compare benefits of laparoscopic artificial insemination over natural breeding and TCAI; 3. describe the procedure; and 3. perform economic analyses and determine the break-even point for LAI service to become profitable. mailto:ramkasi@wsu.edu http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ http://dx.doi.org/10.58292/CT.v17.11080 2 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 Intravaginal (pericervical) artificial insemination Vaginal insemination is performed with fresh or chilled semen. In this method, the semen is deposited in the cranial vagina (fundus) using an infusion rod. The procedure is easy and quick to perform under field conditions and may result in lower but still acceptable pregnancy rates. Vaginal AI is best suited to use after estrus detection during the natural breeding season. The ideal timing of AI is before ovulation (i.e. 12-18 hours after estrus onset);9 recommended semen volume is 0.2-0.5 ml and the number of progressively motile sperm is minimum of 300 × 106.7,10 Intravaginal AI is effective in ewes with fresh semen compared to extended (chilled) semen. However, conception rates after vaginal insemination are lower with pharmacological estrus synchronization. Additionally, pregnancy rates following vag- inal insemination with frozen semen are not acceptable. Cervical insemination with fresh or liquid semen and intra- uterine insemination with frozen semen via laparoscopy require smaller insemination doses. Intracervical artificial insemination Intracervical AI with fresh or chilled semen is the ideal choice in small ruminants since the external cervical os is located/visual- ized with illumination. The insemination pipette is passed through the speculum into the cervix, without excessive force, to a depth of 5-12 mm. Semen is deposited after positioning the pipette in the cervix. Special attention is necessary to mini- mize semen backflow. Unlike intravaginal AI, conception rates achieved with fresh or chilled semen via this method are accept- able after pharmacological estrus synchronization.7,9,11 The ideal time for AI is 48-65 hours after intravaginal progesterone inserts removal or 15-17 hours after the onset of detected estrus.12-14 Originally, use of frozen semen was limited because of the low lambing percentages (25-35%) with cervical AI. This was asso- ciated with the reduced viability of frozen sperm resulting in lower numbers of viable or undamaged sperm reaching fertil- ization site. Currently, with the development of cervical AI skills and good semen evaluation and processing methods, preg- nancy rates are improved.15 Insemination volume and the num- ber of motile sperm required are 0.2-0.5 ml and 150-200 × 106, respectively.7,10 Since LAI is prohibited in most of the North European countries and requiring special and costly equipment and well-qualified technicians, importance is given to cervical AI with frozen semen.16,17 In goats, intracervical AI is the most widely practiced method since caprine cervix is relatively easier to traverse than ovine cervix. Intrauterine artificial insemination Lack of perfect nonsurgical procedures for ewes severely limits its use. Direct transfer of semen into uterine lumen with the laparoscopic technique has resulted in acceptable results, but it has disadvantages. Alternatively, transcervical intrauterine AI techniques (e.g. Guelph system of transcervical AI) have been developed.10,18-24 Transcervical AI procedure require spe- cial positioning of the animal, cervical retraction and stabili- zation, and specially designed instruments for stabilization and passing insemination pipette through the cervix. Recently developed methods allow the semen to be deposited atrau- matically, even deeper into the uterine horns by the transcervi- cal route.20-22 However, the process of manipulating an AI catheter through the cervix still has been linked to reductions in pregnancy and lambing rates.22 It has been hypothesized that cervical trauma and vaginal/cervical stimulation caused by the catheterization may activate pathways interrupting pregnancy between days 3-14.21,22 Intracervical application of hyaluronan 52 hours after sponge removal improved cervical relaxation and increased cervical entry.25 Among other meth- ods, the use of drugs (hormones,26,27 chemokines,28 myorelax- ants,29 beta-adrenergic blocking agents30,31) and the design of inseminating catheters32,33 associated with manipulation of the cervical canal (i.e. Guelph System17-20) have been investi- gated with some success. The procedure of transcervical AI could be even simpler if it were sufficient to deposit the semen in only 1 uterine horn. There are opinions to the contrary,5 but evidence from LAI in sheep indicates that the deposition of semen in a single uterine horn leads to fertilization of ova from both ovaries.34 Insemination volume and the number of  motile sperm required are 0.2-0.5 ml and minimum 100-200 × 106, respectively.7,10 Trained and experienced insem- inators may enter the cervix in 75-85% of ewes.20,21 However, transcervical AI cannot be used with an acceptable success rate in nulliparous ewes. Cervical injury, abscesses, infections and poor pregnancy rates are associated with this technique, but they vary by operator, semen dose, ewe condition, and experi- ence.22,24 Pregnancy rates are generally lower than those achieved by laparoscopic AI (fresh: 40-70%; frozen: 30-70%). Laparoscopic intrauterine AI The novelty of laparoscopic intrauterine insemination of ewes represents the most remarkable development in ovine AI industry. With the laparoscopic technique, decreased amount of semen is deposited into uterine horns lumen. The number of sperm required for each insemination is lower and the volume of semen is proportionally higher.35,36 This allows more suitable dilution rates and, therefore, better preservation and protection of sperm. Laparoscope-aided intrauterine AI improves pregnancy rate achieved with cryo- preserved semen and facilitates the widespread dissemina- tion of valuable genotypes. When correctly performed, this method has a success rate of > 60%, but this rate will vary depending on the quality of semen, the time of the year, the ewe’s condition, and the skill of the inseminators. Conception rates achieved with frozen semen used by this method are Figure 1. Image of mold of ewe’s cervical canal with cervical folds and rings (Courtesy of Dr. Brian Buckrell) a b http://dx.doi.org/10.58292/CT.v17.11080 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 3 higher than for intracervical insemination, because of better cryopreservation of sperm and deposition of semen in the uterus. The main disadvantages are the need for expensive laparoscopic equipment, invasive surgery (animal welfare issues), and the technical expertise needed to perform the procedure. Its cost usually limits this procedure for expensive semen, valuable animals or breeding companies. Current techniques recommend depositing half the total dose of semen in the middle region of each uterine horn without regard to ovulation site;9,37 however, deposition in 1 horn had acceptable conception rates.8,37 In estrus-synchronized ewes, ideal timing of AI is 48-65 hours12-14,37 after the removal of progesterone inserts and 12-18.5 hours38 after estrus detection. Insemination volume and the number of motile sperm required are 0.25 or 0.5 ml and mini- mum of 20-40 × 106, respectively.9,15,16,38 An average ejaculate diluted appropriately can be utilized to inseminate 50 ewes. An experienced team in a well-equipped and organized operation can inseminate 250-500 ewes in a day with this technique. Factors influencing the reproductive outcome Application of AI in sheep production requires appropriate knowledge of animal management, seasonality, breeds involved, and production system of small ruminants. Several factors influ- ence AI program success in small ruminants (Table 1). Sheep breed can influence the length of breeding season; sheep breed should be selected based on operation objective. In accelerated breeding programs (increase the number of lamb crops per ewe; 3 lambing in 2 years and 5 lambing in 3 years) it is ideal to have sheep breeds with a long breeding season. Sheep breeds and breeding season length are provided (Table 2).71,72 Laparoscopic AI Animal selection Careful selection of animals is vital to achieve good reproduc- tive outcome and to deliver the best monetary results to cli- ents. As mentioned above, there are several factors pertinent to ewe and ram, among others that influence LAI results. Selection of ram and ewe provides the best improvement in genetics and conception rate. Implementing AI allow produc- ers with an opportunity to choose genetically superior semen with desirable traits, based on operation objectives. Frozen semen technology provides the ability to obtain semen from genetically top studs throughout the world. However, repro- ductive performances with fresh, cooled, and frozen semen can differ (Table 3). Guidelines for animal welfare Animal welfare is important in all aspects of animal husbandry and veterinary medicine. Animal health and well-being should be considered in breeding protocol and AI. Precautions should be taken to prevent undue stress and pain. Sheep should be sedated for LAI and given preemptive pain medication and pro- phylactic antibiotics.78 Effort should be made to maintain sterility of all instruments entering the abdominal cavity.78 Table 1. Factors influencing the reproductive outcome Factors References Female factors: Age of the ewe/parity 39-41 Genetics 42 Ewes’ body condition 43,44 Prolificacy 45,46 Health management 47 Nutritional management 48-51 Synchronization and induction of estrus 52-54 Stress 55,56 Hygiene 57,58 Animal management 59,60 Male factors: Age of the ram 61 Genetics 42 Scrotal circumference 62,63 Superior quality semen 6,41,61,64,65 Others: Farm 41,66 Year 41 Season 41,66,67 Semen type 68,69 AI technique 66,68 AI technician 41,66 Insemination time, method 6,67,70 Interval from lambing to AI 41 Table 2. Length of breeding season and breed of sheep Length of breeding season Breeds Long (6-8 months) Dorset Finn Polypay Rambouillet Rideau Romanov Medium (4-6 months) Canadian Charollais Hampshire Oxford Suffolk Short (4 months) Leicester North Country Cheviot Scottish Blackface Shetland Texel http://dx.doi.org/10.58292/CT.v17.11080 4 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 The animal should be in the cradle only for a short duration to prevent adverse effects associated with dorsal recumbency. Time spent in the Trendelenburg position should be minimal due to pressure on lungs. Therefore, animals should only be posi- tioned when everything is ready. If an animal develops breath- ing complications, it should be returned to a normal position. After LAI, sheep should be monitored for postanesthetic com- plications. When LAI is performed correctly, complications are mitigated with lower incidence of surgical complications. A comprehensive list of LAI complications is provided.37 Complications include rupture or puncture of abdominal vis- cera, subcutaneous emphysema, hematoma or subcutaneous bleeding, abscess formation, peritonitis, sepsis, intra-abdomi- nal adhesions, and uterine bleeding.37 Synchronization of estrus Synchronization of estrus is vital for AI program success. Ewes are often artificially inseminated at a fixed time. The basis for implementation of synchronization protocols is to hormon- ally regulate the estrous cycle at the herd level; this facilitates scheduling of the procedure at a fixed time and increases fer- tility. Various synchronization protocols are available for small ruminants; protocol used should be customized with the owner’s and veterinarian’s goals and schedules. There are several pharmaceuticals available in the USA for syn- chronization protocols. Prostaglandin (PGF2a) lyses a corpus luteum in cycling ewes to induce estrus and is given twice (7-12 days apart) for luteal regression and estrus expression (Table 4).79-82 Estrus detection is required for the 2-dose PGF2α protocol. Since estrus expression occurs over a period, timed AI is not possible. It was not successful due to poor fertility and embryonic loss83 compared to synchronization protocols with progestogen, progesterone, and eCG;84 however, there was no impact on fertilization.85 Efficiency of PGF2α in combi- nation with the ram effect was explored.86 The most common method of estrus synchronization involves progesterone followed by a single dose of equine chorionic gonadotropin (eCG) or PG600. This requires progesterone, controlled internal drug release (CIDR)73 or compounds with progesterone-like activity (progestogen sponges, MAP, medroxyprogesterone acetate;87,88 or FGA, Fluorogestone ace- tate73,89) in intravaginal devices that are used 12-14 days prior to AI. At removal, a single injection of eCG73,90 or PG60091,92 is given subcutaneously to induce ovulation. Androgenized teas- ers can also be introduced to ewes along with eCG treatment to help stimulate estrus.93,94 Most ewes will enter estrus 24-36 hours later, peaking at 48 hours.95 Laparoscopic intra- uterine AI of ewes with semen is normally performed Table 4. Effect of prostaglandin F2α on estrus response and pregnancy in ewes Prostaglandin F2α Dose Interval (days) Season Type of breeding ER (%) PR (%) Reference Cloprostenol 120 μg 7 Breeding Natural 91 73.3 80 9 94 75.9 11.5 94 75.9 Cloprostenol 126 μg 9 Breeding 100 81 68.25 μg 9 75 38.5 μg 9 87.5 Dinoprost tromethamine 10 mg Single dose Breeding Natural 60 82 Cloprostenol sodium 150 μg 70 D-cloprosternol 150 μg 70 ER: estrus response rate; PR: pregnancy rate Table 3. Reproductive outcome in laparoscopic intrauterine insemination* Semen type Insemination dose (x 106 sperm) Number of ewes Reproductive outcome (%) Season References Fresh 50 in each horn 300 PR 70.1 Breeding 73 N/A 2,508 PR 82.2 Breeding and anestrus 69 Cooled 20 in each horn 265 LR 75.3 Breeding and out of season 74 Frozen 50 103 LR 62.1 Out of season 75 75 243 PR 50.2 Breeding 76 N/A 25,939 PR 70.1 Breeding and anestrus 69 80 198 LR 63.6 Breeding 77 *synchronization regimens and insemination timing referred were different; PR: pregnancy rate, LR: lambing rate http://dx.doi.org/10.58292/CT.v17.11080 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 5 48-65 hours after CIDR removal (depending on whether fresh or frozen-thawed semen is used), with a minimum of 25 × 106 motile sperm deposited per ewe.9 Several types of intravaginal devices resulted in varying repro- ductive outcomes. CIDRs are more accessible, quick to insert, and a more efficient form of progesterone.96 However, protocols must be adapted depending on the progesterone and treatment form. CIDRs resulted in an earlier response and ovulation com- pared to sponges, 55 and 60 hours, respectively.95 The delay in the commencement of ovulation was observed in November and December when most ewes are expected to be in anestrus.95 CIDR treatment produced less variation around ovulation tim- ing97 and a greater tendency for higher conception rates, 69 compared to 58.6% with other intravaginal devices. Further, during breeding season, CIDR had significantly higher preg- nancy, fertility, twinning rate and fecundity compared to sponges during breeding and out of season.73,98 Another study, conducted during the breeding season, had higher fertility yields in short-term (6-7 days) CIDR-based treatments (75%) and the long-term (12-14 days) CIDR-based treatment (70%) than in the long-term (12-14 days) sponge-based treatment (45%).99 Poor fertility in sponge groups was attributed to change in vaginal pH (~ 8) and vaginal microbiome (a higher incidence of Salmonella spp. and Staphylococcus aureus).99 Conversely, the percentage of ewes lambing and twinning rate are similar between sponges and CIDR-treated ewes during breeding and out of season.96,100 FGA sponges resulted in higher pregnancy than CIDRs in autumn (54.7 versus 67.4%), but not spring (59.2 versus 56.2%), suggesting a seasonal impact on the effectiveness of estrus synchronization.101 However, another study reported no difference between pregnancy rates in March, April, and May inseminations compared to summer months.69 The type of progestogen could also be a reason for the variabil- ity. A lower conception rate was observed with MAP (64.6%) compared to FGA (74.7%) or CIDR (71.7%).69 During breeding season, eCG treatment in ewes after proges- terone removal increased rates of ovulation,102,103 conception and lambing.73,103 Treatment with 250 IU (72.9%) or 300 IU (79.1%) eCG doses resulted in higher pregnancy rate com- pared to 200 IU (62.4%). In nonseasonal ewes, a higher eCG dose (400 IU) at CIDR removal normally led to a superovula- tory effect or increase in prolificacy, measured as twins and triplets.101 During breeding season, GnRH treatment at estrus onset or before luteinizing hormone (LH) surge or 36 hours after progesterone device removal,104,105 had no improvement in the number of pregnant ewes compared to current proto- cols97 despite improved synchronization of anestrus ewes.102 GnRH has been used in sexed semen106,107 or in multiple-ovu- lation embryo transfer programs,102,108 where timing of ovula- tion is critical for success. Estrus response and pregnancy for various combinations of progesterone and gonadotropin are provided (Table 5) CIDR is the only approved form of progesterone device in the USA. Currently, eCG is either unavailable or backordered in the USA and PG600 does not perform as well as eCG. Standard doses of PG600, typically intended for pigs, can lead to over- stimulation of the ovaries, resulting in reduced pregnancy rates due to issues with ovulation and embryo survival; there- fore, a lower dose of PG600 is usually recommended for sheep.91,109 Additionally, PG600 in small ruminants is consid- ered extra-label. Therefore, other products, such as GnRH ago- nists (GnRH, hCG94) or FSH,110,111 should also be considered. Combinations of various progesterone and gonadotropin formulations to induce estrus for natural or artificial insemi- nation in ewes are provided (Table 5). Postthaw semen evaluation Evaluation of postthaw semen is important to determine the quality. Although postthaw motility is the primary criterion, evaluation of membrane integrity, viability and functional tests such as hypo-osmatic swelling and sperm DNA and acrosomal integrity are used to objectively evaluate sperm quality. However, in the field, motility and concentration are commonly used indicators to determine semen quality and quantity. Briefly, semen straws are removed from the liquid nitrogen storage tank and placed in a 37°C water bath for 1 minute, then dried with a paper towel. The straws are cut with scissors, and the samples are emptied into glass vials, which are placed on a 37°C digital dry block warmer and swirled for 1 minute. A 7.5 µl sample is then pipetted to the iSperm (Madison, WI, USA) base chip for analysis of total motility and concentration.117 Procedure Laparoscopy requires insertion of a cannula/trocar through the abdominal wall, distension of the abdominal cavity with sterile air or CO2, and visual examination of the abdominal organs with an illuminated telescope. In LAI, 2 ports of entry are created in the abdomen: 1 for the laparoscope to visualize the uterus and the other for passing the AI gun for insemina- tion. Equipment list for transcervical artificial insemination and laparoscopic insemination in small ruminants is pro- vided.118 The procedure is relatively quick once ewes are ready for AI. The procedure can take < 1 minute to a few minutes per animal, depending on experience. It is advisable to have sev- eral working groups: technicians to help sedate ewes, load them into the cradle and prepare ewes for LAI and monitor them; technician to thaw and evaluate semen; personnel to perform AI; and a group to monitor recovery. Sedation can be accomplished through a single intravenous injection of an anesthetic combination. A mixture of 1,000 mg of ketamine, 100 mg of xylazine (α-2 adrenoceptor agonist, Table 6), and 10 mg of butorphanol. A dose of 0.1 ml/20 lbs can sedate a small ruminant long enough to undergo the LAI procedure while maintaining minimal side effects and short recovery times.119 Intramuscular (0.05-0.3) or intravenous (0.016-0.1) xylazine alone or in combination with butorphanol (intravenous xyla- zine [0.1-0.2] + intravenous butorphanol [0.01-0.02]) or (intra- muscular xylazine [0.02] + intramuscular butorphanol [0.05-0.07] are used.121,122 Xylazine and other α-2 agonists have several dose-dependent adverse effects and require careful con- sideration.123 For instance, small ruminants are particularly sen- sitive to the pulmonary effects of these drugs. The primary cause of these pulmonary effects is the activation of pulmonary intra- vascular macrophages (PIMs). Once activated, PIMs release prostaglandins and other vasoactive substances leading to alve- olar edema, increased transpulmonary pressure, reduced pul- monary compliance, and pulmonary congestion. Ketamine (1 mg/kg) combined with xylazine (0.05 mg/kg; 20 mg/ml) and butorphanol (0.025 mg/kg) are used to make a stock solution for the LAI procedure.120 Ewes are given intra- muscularly 0.02 ml/kg. After 10 minutes, the sedated females are ready to stand alongside of an artificial insemination table and for rolling into dorsal recumbency. http://dx.doi.org/10.58292/CT.v17.11080 6 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 Ta b le 5 . E ff ec ts o f c o m b in at io n s o f v ar io u s p ro ge st er o n e an d g o n ad o tr o p in fo rm u la ti o n s o n e st ru s re sp o n se a n d p re gn an cy fo ll ow in g n at u ra l o r ar ti fi ci al in se m in at io n in e w es P ro ge st er o n e P 4 tr ea tm en t d u ra ti o n (d ay s) G o n ad o tr o p in s p ro st ag la n d in s Se as o n B re ed in g ty p e ER ( % ) P R ( % ) R ef er en ce FG A 4 0  m g 14 eC G 2 00 I U a t P 4 w it h d ra w al B re ed in g se as o n N at u ra l 1: 5 - ra m :e w e 93 .3 60 .0 89 FG A 4 0  m g 5 eC G 2 00 I U a t P 4 w it h d ra w al + c lo p ro st en o l 1 00 m g at P 4 w it h d ra w al 86 .7 86 .7 FG A 4 0  m g 5 C lo p ro st en o l 1 00 m g o n d ay 0 + e C G 2 00 I U a t P 4 w it h d ra w al 92 .3 92 .3 FG A 4 0  m g 5 C lo p ro st en o l 1 00 m g o n d ay 0 + G n R H 1 00 m g 30 h o u rs af te r P 4 w it h d ra w al 66 .6 33 .3 - - G n R H 1 00 m g o n d ay 0 + c lo p ro st en o l 1 00 m g o n d ay 5 + 20 0 IU o n d ay 5 91 .7 66 .7 FG A 4 0  m g 5 C lo p ro st en o l 1 00 m g o n d ay 5 a t P 4 w it h d ra w al B re ed in g se as o n TA I (+ 5 2 h o u rs o r + 60 h o u rs a ft er P 4 w it h d ra w al ) 52 h o u rs : 5 0. 0 60 h o u rs : 3 3. 3 89 FG A 4 0  m g 5 C lo p ro st en o l 1 00 m g o n d ay 5 a t P 4 w it h d ra w al + G n R H (1 00 m g gi ve n 3 0 h o u rs a ft er P 4 w it h d ra w al 52 h o u rs : 4 0. 0 60 h o u rs : 2 0. 0 FG A 4 0  m g 5 C lo p ro st en o l 1 00 m g o n d ay 5 + e C G 2 00 I U o n d ay 5 a t P 4 w it h d ra w al + G n R H ( 10 0 m g gi ve n 3 0 h o u rs a ft er P 4 w it h d ra w al 52 h o u rs : 2 0. 0 60 h o u rs : 6 0. 0 C ID R 0 .3  g 14 eC G 6 00 I U a t P 4 w it h d ra w al B re ed in g se as o n TL A I 96 .9 77 .8 73 FG A 3 0  m g 14 eC G 6 00 I U a t P 4 w it h d ra w al 95 .7 62 .4 M A P 6 0  m g 12 C o n tr o l O u t o f b re ed in g se as o n N at u ra l 1: 6 - ra m :e w e -  5 6 88 M A P 6 0  m g 12 eC G 5 00 I U a t P 4 w it h d ra w al -  8 8 C ID R 0 .3 g 14 eC G 4 00 I U a t P 4 w it h d ra w al O u t o f b re ed in g se as o n Tr an sc er vi ca l A I 10 0. 0 75 .0 90 G n R H 4 00 m g at P 4 w it h d ra w al 10 0. 0 75 .0 G n R H 5 00 m g at P 4 w it h d ra w al 10 0. 0 70 .0 G n R H 6 00 m g at P 4 w it h d ra w al 10 0. 0 75 .0 h C G 2 00 I U a t P 4 w it h d ra w al 10 0. 0 75 .0 h C G 3 00 I U a t P 4 w it h d ra w al 10 0. 0 80 .0 h C G 4 00 I U a t P 4 w it h d ra w al 10 0. 0 75 .0 C ID R 0 .3 g 9 C lo p ro st en o l 1 20 m g o n d ay 7 + P G 60 0 5 m l B re ed in g se as o n N at u ra l m at in g 3: 16 – r am :e w e - 77 .0 91 C lo p ro st en o l 1 20 m g o n d ay 7 + P G 60 0 1. 5 m l - 87 .5 C lo p ro st en o l 1 20 m g o n d ay 7 o n ly - 37 .5 (C on ti nu ed ) http://dx.doi.org/10.58292/CT.v17.11080 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 7 Ta b le 5 . ( C on ti nu ed ) P ro ge st er o n e P 4 tr ea tm en t d u ra ti o n (d ay s) G o n ad o tr o p in s p ro st ag la n d in s Se as o n B re ed in g ty p e ER ( % ) P R ( % ) R ef er en ce C ID R 0 .3 g 5 3 m l P G 60 0 (2 40 I U e C G , 1 20 I U h C G ) at P 4 w it h d ra w al A n es tr u s N at u ra l m at in g 1: 25 – r am :e w e 79 .2 71 .9 92 C o n tr o l 94 .2 66 .7 C ID R 0 .3 g 5 3 m l P G 60 0 (2 40 I U e C G , 1 20 I U h C G ) 1 d ay b ef o re P 4 w it h d ra w al 79 .6 51 .7 C o n tr o l 84 .3 59 .9 FG A 4 5  m g 11 0. 1 m g o f P G F 2α o n d ay + 6 μ g o f in tr am u sc u la r G n R H 3 6 h o u rs af te r sp o n ge w it h d ra w al B re ed in g C er vi ca l A I 70 .0 71 .4 54 11 33 0 IU a n d 0 .1 m g o f P G F 2α d ay 9 83 .3 80 .0 11 33 0 IU a n d 0 .1 m g o f P G F 2α o n d ay 9 + 6 μ g o f in tr am u sc u la r G n R H 3 6 h o u r af te r sp o n ge w it h d ra w al 86 .7 80 .8 13 0. 1 m g o f P G F 2α o n d ay 1 2 + 30 0 IU a t sp o n ge w it h d ra w al 80 .0 75 .0 13 30 0 IU a t sp o n ge w it h d ra w al 76 .7 73 .9 1 M A P 6 0  m g 14 eC G 3 00 I U a t P 4 w it h d ra w al N o n b re ed in g N at u ra l 80 83 .3 11 2 M A P 6 0  m g 14 eC G 6 00 I U a t P 4 w it h d ra w al 80 10 0 C o n tr o l - - 10 0 M A P 6 0  m g 6 eC G 3 00 I U + 75 μ g D -c lo p ro st en o l, 1 d ay b ef o re P 4 w it h d ra w al B re ed in g N at u ra l 72 .7 45 .5 11 3 9 eC G 3 00 I U + 75 μ g D -c lo p ro st en o l, 1 d ay b ef o re P 4 w it h d ra w al 72 .7 36 .4 12 eC G 3 00 I U + 75 μ g D -c lo p ro st en o l, 1 d ay b ef o re P 4 w it h d ra w al 80 20 FG A 4 0  m g 12 eC G 1 40 I U 1 d ay b ef o re P 4 w it h d ra w al N o n b re ed in g N at u ra l 10 0 70 .0 11 4 FG A 4 0  m g 12 eC G 2 80 I U 1 d ay b ef o re P 4 w it h d ra w al 10 0 73 .7 FG A 4 0  m g 10 C O N B re ed in g N at u ra l 89 62 .5 a 10 3 FG A 4 0  m g 10 eC G 1 00 I U 92 .7 58 .8 a FG A 4 0  m g 10 eC G 2 00 I U 95 .8 93 .7 b FG A 4 0  m g 10 eC G 4 00 I U 91 .7 10 0b FG A 4 0  m g 14 eC G 5 00 I U B re ed in g A I 10 0 90 .6 b c 11 5 FG A 4 0  m g 14 eC G 6 00 I U 10 0 93 .7 b FG A 4 0  m g 14 eC G 7 50 I U 10 0 10 0a C o n tr o l - - 97 .1 79 .4 c FG A 4 0  m g 12 eC G 3 00 I U B re ed in g N at u ra l 64 36 11 6 eC G 4 50 I U 77 .7 19 .3 eC G 6 00 I U 86 .3 36 eC G 7 50 I U 80 .9 19 C ID R : c o n tr o ll ed in te rn al d ru g re le as e; F G A : fl u o ro ge st o n e ac et at e; M A P : m ed ro xy p ro ge st er o n e ac et at e; E R % : e st ru s re sp o n se r at e; P R % : p re gn an cy r at e; A I: a rt ifi ci al in se m i- n at io n ; T A I: t im ed a rt ifi ci al in se m in at io n ; T LA I: t im ed la p ar o sc o p ic a rt ifi ci al in se m in at io n ; t re at m en ts w it h o u t co m m o n s u p er sc ri p ts d if fe re d ( p < 0 .0 5) . http://dx.doi.org/10.58292/CT.v17.11080 8 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 Intramuscular (0.05-0.2 mg/kg) or intravenous (0.02-0.05 mg/kg) acepromazine is the most used agent in this group. Compared to xylazine, it has a delayed onset of action. The maximum effect after intravenous treatment may take 15-20 minutes, and its duration of action lasts 4-6 hours. Although acepromazine has minimal respiratory effects, it may cause arterial hypotension, especially at higher doses or in hypovo- lemic animals. Furthermore, acepromazine lacks analgesic properties, and its sedative effect is weaker than xylazine. Acepromazine is most effective when used in combination with other drugs. Benzodiazepines (intravenous diazepam: 0.5-1.0 mg/kg given slowly, and intravenous, subcutaneous, or intramuscular midazolam 0.3-0.5 mg/kg) have minimal effects on the cardiovascular and respiratory systems;124 used for sedation in small ruminants as the sole drug, but they are preferred in combination with other agents.125 Once ewes are sedated and loaded onto the cradle on dorsal recumbency, the posterior ventral abdomen (30 × 30 cm area cranial to the udder) is surgically prepared (clipped and scrubbed with chlorhexidine- and sterile saline-soaked gauze). Once the site is sterilized, injection of local anesthetic (2% lidocaine) is given 5 inches cranial to the teats and 2 inches off the midline on each side with care to avoid large veins. After a few minutes (for the local anesthetic to take effect), the cradle is lifted to a 40 degree angle, with the abdomen at a higher elevation than the head (Figure 2). A small incision through the skin (but not the abdominal wall) is made in each of the spots where the local anesthetic was injected. A trocar is care- fully passed through the abdominal wall along with cannula and removed to insufflate the abdomen with CO2 before pass- ing a second trocar through the second incision. The trocar is removed from the second cannula and a laparoscope is passed through 1 canula while forceps is passed through the other (Figure 2). Once the uterus is visualized through the laparo- scope, it is manipulated into position for insemination with forceps. Once the uterus is secured in a desirable position, the forceps is withdrawn and replaced with the insemination gun loaded with semen. The insemination gun is used to puncture and deposit semen in both uterine horns at the midway point between the utero-tubal junction and the base of the horn. Once this is accomplished, equipment is withdrawn from the abdomen, abdomen deflated, and incisions are closed with staples, sutures, or tissue glue; however, sutures and staples are avoided.37 Ewes should be allowed to recover in a quiet pen while monitored for adverse effects. In general, ewes readily walk away. Intravenous yohimbine (0.1-1.0 mg/kg) is the anti- dote for xylazine to reverse effects. Intramuscular or intrave- nous atipamezole may also be used for reversal (0.1-0.2 mg/kg). Diazepam and midazolam are reversed with intramuscular or intravenous flumazenil (0.02 mg/kg).126 Estimated input cost for laparoscopic artificial insemination service Purchasing the reusable equipment to perform LAI is an investment upfront but can quickly turn into profit. To per- form LAI, it is necessary to have the equipment to enter the abdomen, visualize the uterus and deposit the semen in the Table 6. α-2 adrenoceptor agonist and doses120 Drug Treatment route Dose Dexmedetomidine (μg/kg) intravenous intramuscular 5-10 10-30 Detomidine (μg/kg) intravenous intramuscular 3-20 20-30 Romifidine (μg/kg) intravenous intramuscular 3-40 40-80 Xylazine intravenous intramuscular 0.016-0.1 0.05-0.3 Figure 2. Laparoscopic artificial insemination procedure in an ewe (Courtesy of Dr. Salman Waqas) http://dx.doi.org/10.58292/CT.v17.11080 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 9 lumen of the uterus. Although 5.0, 6.5, 7.0, 7.5, and 10 mm laparoscopes are available, 5 mm is a popular choice among veterinarians and will be the size of equipment mentioned unless otherwise mentioned. Purchasing refurbished or used medical equipment has been a popular choice and its price is used in our cost analysis. Necessary reusable equipment includes, laparoscope, trocars, cannulas, a CO2 tank, scope, light source, forceps, a laparoscopy cradle, LAI gun, and semen processing equipment. Laparoscope with light source and forceps: cost of a laparoscope can vary depending on whether it provides digital imaging or a lens, degree of offset, and type of light source; new $7,000 or used for $2,500. A 0-degree scope with a lens is ~ $250. A used halogen light source with a fiber optic cable is ~ $1,000 or a portable handheld endoscope light source (~ $150) may be more suitable for field use. Laparoscopic forceps is required to manipulate the uterus or omentum before insemination and can be purchased for ~ $100. Trocar and cannulas: available in 3 sizes: 5, 7.5, and 10 mm. It is desirable to have smaller size cannulas as it allows for smaller incisions and smaller rigid 5 mm laparoscopes, require no trocar adaptors for AI guns, carry reduced risks of abdomi- nal perforation and prolonged lifespan due to lesser wear and tear. Trocars and cannulas with a port are required to enter and insufflate the abdomen; 2 are needed as 1 will be for the scope and the other for the forceps to manipulate the uterus or insemination pipette. A set of these can be ~ $500 for a new set or $250 for a used set. CO2 tank: is required to insufflate the abdomen. Insufflating the abdomen allows for more space to manipulate and visual- ize the uterus. A medical grade insufflation air unit or a generic tank and regulator is used. Medical grade unit, CO2 tank with regulator, and a commercial vacuum pump can be purchased for $8,750. A used tank and regulator with a hose can be pur- chased for ~ $300. Cradle: is needed to hold the sheep in dorsal recumbency at an incline during the procedure. A new cradle can be pur- chased for ~ $1,800. Used units are not always readily avail- able but can sometimes be purchased for < $1,000. Although 2 cradles are ideal, only 1 cradle was considered for economic analysis. An LAI gun for use with Robertson insemination pipette, sheaths and semen straws (Minitube USA, Inc, Verona, WI, USA) can be purchased as a new set for $400 or conversely a less costly Robertson insemination pipette with a syringe can be used for semen deposition. Other available options include laparoscopic sheaths (Biogenesis, Bloomington, IL, USA) for universal small ruminant AI gun and ovine transcap guide with aspic sheath for mini straws (IMV Technologies, 61300 L’Aigle, France). Semen processing equipment can include a microscope, a slide warmer, semen straw cutters, and a semen thawing unit. A generic microscope can be purchased for ~ $300, or a semen specific microscope for ~ $2,500. A slide warmer can be pur- chased for ~ $200. Semen straw cutters are relatively inexpen- sive at $15 each. A semen thawing unit can be purchased for ~ $200. Cost of new and refurbished laparoscopic AI equipment is provided (Table 7). Costs associated with training (such as course fee, travel, hotel, etc.) and practicing on smaller flocks to gain proficiency are key factors in calculating breakeven costs. However, these fac- tors are not included in this economic analysis because train- ing costs and the time required to train an individual can vary substantially. In general, a set of refurbished equipment can be purchased for ~ $4,000. Fiber optic light sources and scopes with digital image abilities will increase the cost and are often not neces- sary. Buying new equipment or job-specific equipment can also increase the cost but may increase the lifespan of the equipment. Many companies sell LAI kits (e.g. ‘Sheep LAI set’ from MEDIT) with fiber optic light source, 0-degree scope, and 2 trocars and cannulas for $2,300. These can be a viable choice for veterinarians who like to upgrade their kit or purchase new equipment guaranteed to be compatible. Consumables are also needed to perform LAI. Consumables can include insemination pipettes and semen. Robertson insemination pipettes can be purchased in bundles and the cost is ~ $20 or less per pipette. Semen cost is variable, depend- ing on the breed and source. Breakeven cost assessment (refurbished equipment procurement with laparoscopic artificial insemination service) Breakeven point (Table 8) at which LAI service would become profitable was determined using current market prices, with the following assumptions. Veterinarian made only 1 trip to inseminate required number of ewes over a 3-hour period, ~ 25 ewes/hour. Expenses - Used/refurbished equipment cost - $4,000.00 - - Technician - $18.00/hour - Trailer rental - $15.00/hour - Needle/syringe/blade/glue - $3.00/ewe - Sedation - $0.50/ewe - Synchronization drug cost - $19.00/ewe (CIDR, PG600 and PGF2α) Table 7. Cost of new and refurbished laparoscopic AI equipment Equipment New ($) Used/refurbished ($) Laparoscopy 7,000 2,500 Light source 7,000 1,400 Trocar/Canula 500 250 Video camera and screen 3,500 - Air insufflation unit 8,500 300 Cradle 1,800 1,000 LAI gun 500-550 200 Semen processing 3,000 715 http://dx.doi.org/10.58292/CT.v17.11080 10 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 Veterinary service revenue - LAI service charge - $80.00/ewe - Farm Call - $75.00/trip Performing LAI in 71 ewes would breakeven the expenses needed to procure the refurbished laparoscopic equipment. Economic advantages of laparoscopic AI over natural and transcervical AI Economic advantage of LAI over TCAI and natural service was determined using a crude economic analysis (Table 9a, 9b, 9c) with following assumptions. Assumptions used for laparoscopic artificial insemination: LAI was performed in 10 ewes with 70% pregnancy rate and 1.6 lambs/ewe (show-quality lambs) - Ram lamb: ewe lamb ratio 50:50 - Cost for frozen semen - $ 100/insemination dose - Feeding lambs to 90 lbs - $ 52.50/lamb - Technician - $ 18.00/hour - Trailer rental - $ 15.00 - Needle/syringe/blade/glue - $ 3.00/ewe - Sedation - $ 0.50/ewe - Synchronization drug cost - $ 19.00/ewe (CIDR, PG600 and PGF2α) - Market price for ram lamb $1,100/lamb - Market price for ewe lamb $1,000/lamb Assumption used for transcervical artificial insemination: - TCAI was performed in 10 ewes with 25% pregnancy rate and 1.6 lambs/ewe (show quality lambs) - Ram lamb: ewe lamb ratio 50:50 - Cost for frozen semen $100/insemination dose - Feeding lambs to 90 lbs $ 52.50 - Technician - $ 18.00/hour - Trailer rental - $ 15.00 - Needle/syringe/blade/glue - $ 3.00/ewe - Sedation - $ 0.50/ewe - Synchronization drug cost - $ 19.00/ewe (CIDR, PG600 and PGF2α) - Market price for ram lamb $1100 - Market price for ewe lamb $1000 Assumptions for natural breeding: - Natural breeding was performed in 10 ewes with 66% pregnancy rate and 1.6 lambs/ewe (show quality lambs) - Ram lamb: ewe lamb ratio 50:50 - Ram value $7500.00 - Feeding lambs to 90 lbs $52.50/lamb - Feeding ram for a year $700.00/ram - Synchronization drug cost $19.00/ewe (CIDR, PG600 and PGF2α) - Market price for ram lamb $600/lamb - Market price for ewe lamb $600/lamb Breakeven point at which LAI services become profitable was determined using current market prices (Table 8); analysis assumed that veterinarian made only 1 trip and inseminated 71 sheep in 3 hours. Cost to benefit for LAI, TCAI, and natural breeding from a pro- ducer’s standpoint are compared (Tables 9a-c). Though natu- ral service and transcervical AI breeding programs produced ~ $2,000 profit, laparoscopic AI produced ~ an additional $6,800 monetary benefit. In addition to monetary benefits, LAI has several others. Although there is a higher overhead cost associated with LAI over more conventional AI methods, it is still a Table 8. Economic analysis of breakeven (expense and profit) cost associated with procurement of refurbished laparoscope and laparoscopic artificial insemination service Itemized list Price ($) per Quantity Cost ($) Revenue ($) Expense: Laparoscope 4,000.00 1 4,000.00 Technicians 18.00/hour; 3 hours 2 108.00 Trailer rental 15.00/hour; 3 hours 1 45.00 Consumables/ewe Needle/Syringe/blade/glue 3.00 71 413.00 Sedation (60 kg ewe) 0.50 71 35.50 Synchronization (CIDR-S, PG600, PGF2α) 19.00 71 1349.00 Total expenses: 5,750.50 Charges LAI 80.00 71 5,680.00 Farm call 75.00 1 $75.00 Total Revenue 5,755.00 http://dx.doi.org/10.58292/CT.v17.11080 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 11 practical option for many veterinarians. The technical skills and equipment required for LAI are more substantial than TCAI but the benefits of LAI include bypassing the cervix, access to a deeper site of semen deposition, more efficient use of sperm, and higher conception rates trans- lating to added value service delivered to clients. Frozen semen dose for LAI is 20-25 × 106 sperm (300-400 × 106 and 150-200 × 106 for transvaginal and transcervical AI, respectively). Sexed semen option is available for sheep and is worth consid- ering. However, it should be based on the producer’s goals; it is vital to perform a cost benefit analysis to assess the value of sexed semen in each specific scenario. Conclusion Laparoscopic artificial insemination is a highly effective and proven method of artificial insemination that has several advantages over traditional insemination techniques. One of the primary benefits of LAI is its precision and ability to achieve higher success rates that translates into improved reproductive outcomes for livestock producers. Although there is an initial investment required to purchase the spe- cialized equipment, the costs are relatively modest when Table 9a. Economic analysis for profit estimation generated after implementing laparoscopic artificial insemination procedure (expenses and revenues considered, based on the assumptions listed*) Itemized List Price ($) Per Quantity Revenue ($) Cost ($) Profit ($) Expenses: Farm call 75.00 1 trip 75.00 LAI service charge 80.00 10 ewes 800.00 Cost of frozen semen 100.00 10 doses 1,000.00 Synchronization drug 19.00 10 ewes 190.00 Needle/syringe/blade/glue 3.00 10 30.00 Sedation 0.50 10 5.00 Trailer rental 15.00 1 15.00 Technician 18.00/hour 1 18.00 Feeding lambs to 90 lbs (52.50) 11* 577.50 *Total lambs (Pregnancy rate 70%; 1.6 lambs/ewe) - 11.2 Profit: Sale of ram lambs 1,100.00 5 5,500.00 Sale of ewe lambs 1,000.00 6 6,000.00 Total 11,500.00 2,710.50 8,789.50 Table 9b. Economic analysis for profit estimation generated after implementing transcervical artificial insemination procedure (expenses and revenues considered, based on the assumptions listed*) Itemized List Price ($) Per Quantity Revenue ($) Cost ($) Profit ($) Expenses: Farm call 75.00 1 trip 75.00 TAI service charge 60.00 10 ewes 600.00 Cost of frozen semen 100.00 10 doses 1,000.00 Synchronization drug 19.00 10 ewes 190.00 Needle/syringe/blade/glue 3.00 10 30.00 Sedation 0.50 10 5.00 Trailer rental 15.00 1 15.00 Technician 18.00/hour 1 18.00 Feeding lambs to 90 lbs (52.50) 4* 210.00 *Total lambs (Pregnancy rate 25%; 1.6 lambs/ewe) - 4 Profit: Sale of ram lambs 1,100.00 2 5,500.00 Sale of ewe lambs 1,000.00 2 6,000.00 Total 4,200.00 2,143.00 2,057.00 http://dx.doi.org/10.58292/CT.v17.11080 12 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 compared to the long-term advantages. Additionally, the overhead costs associated with this service are minimal, making it an economically viable option for veterinary prac- tices. For practitioners who already have a sufficient client base, the equipment expenses can often be recouped within just 1 breeding season, providing a strong return on investment. However, several factors must be carefully considered to ensure the success of artificial insemination in sheep. These include the quality of the semen used, proper storage condi- tions, animals’ fertility level, effective management practices, and strict adherence to scheduling protocols. Additionally, producer compliance with recommended procedures and the timing of insemination relative to the breeding season are all critical to achieving the desired outcomes. It is essential that these factors are monitored and controlled to minimize risks and optimize results. Any variation or oversight in these areas can significantly impact the success rates of the procedure. The economic benefits of laparoscopic artificial insemina- tion are substantial, especially to veterinarians who can pro- vide this service to a broader client base. By increasing conception rates and reducing the need for costly repeated matings, LAI can ultimately save producers money while also contributing to higher overall productivity. For veterinary practices, adding LAI procedures provides a valuable oppor- tunity to differentiate themselves in a competitive market. Effectively communicating these benefits to clients is crucial, as it helps to underscore the advantages of the service and fosters trust between practitioners and producers. LAI not only enhances the economic viability of a practice but also adds a layer of expertise and specialization, further enriching the practice’s reputation and service provided. Conflict of interest None to report. Author contribution Authors equally contributed to this work. References 1. 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Economic analysis for profit estimation generated after implementing natural service breeding program (expenses and revenues considered, based on the assumptions listed*) Itemized List Price ($) Per Quantity Revenue ($) Cost ($) Profit ($) Expenses: Cost of the ram 7,500.00 1 7,500.00 Feeding ram for 1 year 700.00 1 700.00 Ram BSE 45.00 1 45.00 Synchronization drug 19.00 10 ewes 190.00 Feeding lambs to 90 lbs (52.50) 11* 577.50 *Total lambs (66% pregnancy rate; 1.6 lambs/ewe) 11 Profit: Ram lambs 600.00 5 3,000.00 Ewe lambs 500.00 6 3,000.00 Sale price of ram after 1 year 5,000.00 1 5,000.00 Total 11,000.00 (9,012.50) 1,987.50 http://dx.doi.org/10.58292/CT.v17.11080 https://doi.org/10.1016/j.theriogenology.2020.01.041 https://doi.org/10.1016/j.theriogenology.2020.01.041 https://doi.org/10.2527/animalsci2002.80E-Suppl_21a https://doi.org/10.2527/animalsci2002.80E-Suppl_21a https://doi.org/10.1017/s002185960000530x https://doi.org/10.1017/s002185960000530x https://doi.org/10.1016/j.anireprosci.2024.107453 https://doi.org/10.1111/j.1751-0813.1982.tb02737.x https://doi.org/10.1111/j.1751-0813.1982.tb02737.x https://doi.org/10.1016/j.theriogenology.2005.02.017 https://doi.org/10.1556/AVet.2012.010 https://doi.org/10.1016/j.cryobiol.2016.11.012 https://doi.org/10.1016/j.cryobiol.2016.11.012 Citation: Clinical Theriogenology 2025, 17, 11080, http://dx.doi.org/10.58292/CT.v17.11080 13 11. 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