1 CONTACT Karen Moran morankaren89@gmail.com © 2025 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http:// creativecommons.org/licenses/by-nc/4.0/), permitting all noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Citation: Clinical Theriogenology 2025, 17, 12711, http://dx.doi.org/10.58292/CT.v17.12711 Case Report Testicular degeneration, fibrosis, and mineralization in Limangus bulls Karen Moran,a,b Raúl Picco,c Eleonora Morrell,d Andrea Verna,d Luis Zapata,b Laura Moiraghi,b Guillermina Bilbao,a,b Julián Bartoloméb aComisión Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina bFacultad de Ciencias Veterinarias, Universidad Nacional de La Pampa, General Pico, Argentina cActividad privada, Villa Huidobro, Argentina dInstituto Nacional de Tecnología Agropecuaria, Balcarce, Argentina Abstract A group of Limangus heifers had a high pregnancy rate after timed artificial insemination but low final pregnancy rate after 75 days of natural service with 2 Limangus bulls. Bulls (A and B) suspected of subfertility and 2 other bulls (C and D) with the same genetic background were examined. Bull A was clinically normal but had no sperm in the ejaculate. Bulls B and C had abnormal testicular consistency resembling fibrosis on palpation and ultrasonography; however, semen was normal. Bull D was clinically normal. Bulls were negative for brucellosis and venereal diseases. Bulls A, B, and C were culled and slaughtered. Bull A had a normal genital tract; however, nonstructured 5B gene was detected by reverse transcription polymerase chain reaction, suggesting an infection with bovine viral diarrhea virus-1 (pestivirus A). Bulls B and C had macroscopic and microscopic signs of testicular degeneration, fibro- sis, and mineralization. Although the specific cause for testicular lesions in bulls B and C was not identified, these lesions were probably responsible for the low pregnancy rate. Keywords: Bulls, infertility, testes, degeneration, fibrosis, mineralization Background A common reproductive strategy in beef herds is to use timed artificial insemination (TAI) at the beginning of the breeding season and 15 days after TAI, use natural service (exposing cows or heifers to bulls for 60 days).1 Various factors affect bull fertility; breeding soundness examination (BSE) is of great value to recognize the fertility of bulls and to maximize the reproductive efficiency of cow-calf operations.2 A standard BSE identifies bulls with substantial deficits in fer- tility but does not consistently identify subfertile bulls.3 Routine BSE includes physical examination, estimation of sperm production based on scrotal circumference (SC), evalu- ation of semen quality, and occasionally, libido or copulation ability is assessed.4 Ultrasonography is a complementary tool to identify testicular lesions that are not detected by palpa- tion.5 In addition, laboratory investigation of venereal dis- eases (Tritrichomonas foetus and Campylobacter fetus venerealis) should be included, since bulls maybe asymptomatic but infected cows will have signs of infertility. Other infectious diseases may affect fertility of beef herds; therefore, core vacci- nation is recommended for bovine viral diarrhea (BVD), infectious bovine rhinotracheitis, leptospirosis, and clostridial diseases.4 We evaluated 4 Limangus bulls during the investigation of infertility in a cow-calf operation located in the south of Cordoba province, Argentina. In the fall of 2018, Limangus heifers (n = 62) were subjected to TAI and then exposed to natural service 15 days later with 2 Limangus bulls. During natural service, more heifers (n = 37) were added to the group. Pregnancy per TAI was 74% (46/62); however, pregnancy for 75 days of natural service was only 69.8% (37/53). In the win- ter of 2019, another group of Limangus heifers (n = 92) were bred and then exposed to natural service 15 days later with the same 2 bulls. Pregnancy per TAI was 50% (46/92); however, pregnancy for 75 days of natural service was only 63% (29/46). The referring veterinarian suspected infertility after detecting low SC and abnormal testicular consistency, since heifers were in good body condition and cycling. Two months later, a complete BSE (including ultrasonography of testis) mailto:morankaren89@gmail.com http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ http://dx.doi.org/10.58292/CT.v17.12711 2 Citation: Clinical Theriogenology 2025, 17, 12711, http://dx.doi.org/10.58292/CT.v17.12711 was conducted and preputial swabs for venereal diseases and blood samples were collected in these 2 bulls (A and B) and 2 other bulls (C and D) that were recently included. Bulls A and B were 3 years and bulls C, and D were 2 years (confirmed by second and first pair of incisors, respectively). Breeding soundness examination and complementary studies Bulls underwent BSE according to criteria;4 accessory sex glands were assessed by transrectal palpation. External reproductive organs, scrotal temperature, and painful responses were evalu- ated by palpation. SC was measured by pushing the testis to scrotal bottom by placing the thumb and fingers laterally on the side of scrotal neck and then pushing ventrally. A metal tape was applied over the scrotum and its contents; SC (in cm) was taken at the greatest diameter of the scrotal contents. Genital tract ultrasonography was conducted with a B-mode ultrasound scanner (Honda HS 1600) with a 7.5 MHz linear array trans- ducer using described methodology.6 High-quality ultrasono- graphic gel was used to generate clear ultrasonograms. One testis was pushed upwards (out of the transducer’s ultrasound wave) while the other testis was pushed downwards for exam- ination, thus scrotal wall was stretched improving the contact between transducer and scrotum. Transducer was applied longi- tudinally on the scrotal skin to evaluate the testis halfway between the head and epididymis tail.6 Semen samples were collected by electroejaculation and eval- uated for sperm motility and morphology. Clean, prewarmed microscope slides, and coverslips were prepared. Sperm motil- ity was evaluated subjectively under brightfield microscopy (400 x); morphology was evaluated using semen smears stained with eosin and nigrosin (E/N) and observed under oil immersion with brightfield microscopy (1,000 x). Sperm (n = 200) were evaluated for head abnormalities in each smear.4 In addition, semen was diluted in 10% neutral buffered formalin (wet mount) and sperm (n = 100) were evaluated for tail defects, abnormal acrosomes, detached sperm heads, and proximal droplets using differential interface contrast micros- copy (1,000 x).7 Serologic testing for Brucella abortus was per- formed using buffered acidified plate antigen assay and preputial swab was obtained for testing sexually transmitted diseases (Tritrichomonas foetus and Campylobacter fetus) using a polymerase chain reaction (PCR) test. A month later, bulls that failed BSE (A, B, and C) were culled and slaughtered; genital tracts were examined. Samples of tes- tis (dorsal, mid, and ventral regions) were frozen at –20°C in sterile vials until processed for BVD virus.8 In addition, 2 mm2 samples were taken for histopathological evaluation: 1. dorsal, middle, and ventral regions of testis; 2. head, body, and tail epididymis; 3. vas deferens; 4. vesicular glands; and 5. lesions. Samples were fixed in Bouin’s solution for 6 hours, then placed in 10% formaldehyde solution (with 3 previous washes) and submitted for histology. Fixed tissues were trimmed and sub- jected to routine paraffin processing. Tissue sections were stained with E/N and examined by brightfield microscopy. BSE and necropsy findings Bull A BSE: Scrotum had a distinct neck; testes were symmetrical with normal tone and were freely moveable. Epididymis and sper- matic cords were within normal limits. SC was 35.5 cm, and the scrotal temperature was normal without painful responses. Internal reproductive organs, prepuce, and penis were within normal limits. Ultrasonography revealed small focal hypere- choic areas (Figure 1A) affecting < 1 % of the area examined. Semen (5 ml) collected via electroejaculation was without sperm. Necropsy: Samples were collected from the left and right epi- didymal tails. Sperm from the left had fair gross motility; individual motility was 70% and in E/N-stained slides 98.5% sperm were morphologically normal with ~ 3% head defects. Morphologically normal sperm on the wet mount was 93% (1% proximal droplet, 2% detached heads, 2% midpiece defects, and 2% principal piece defects). Sperm from the right had good gross motility; individual motility was 70% and in E/N-stained slides 99% sperm were morphologically normal with ~ 2% head defects). Morphologically normal sperm on the wet mount was 96% (4% had principal piece defects). Small focal white streaks of < 3 mm2 (Figure 1B) were observed in both testicular histology. Epididymis, vas deferens, ampullae, and vesicular glands were within normal limits. Stromal fibrosis (Figure 1C) was evident in left testis. Ampullae and vesicular glands had mild fibrosis. In addi- tion, nested multiplex reverse transcription polymerase chain reaction (RT-PCR) identified nonstructured 5B (NS5B) gene of BVD virus (BVDV-1 [pestivirus A]). Bull B BSE: Scrotum had a distinct neck; testes were symmetrical and were freely moveable. Right testis consistency was abnormal and had palpable intratesticular firm nodules. Epididymis and spermatic cords were within normal limits. SC was 35.5 cm, and the scrotal temperature was normal without painful Figure 1. Bull A: A. Ultrasonogram of left testis; note small hyperechoic areas (black arrows). B. Cut section of testes; note small < 3 mm2 in both testes (black arrows). C. Light microscopic image (1000 x) of left testis; note stromal fibrosis (black arrow) http://dx.doi.org/10.58292/CT.v17.12711 Citation: Clinical Theriogenology 2025, 17, 12711, http://dx.doi.org/10.58292/CT.v17.12711 3 responses. Internal reproductive organs, prepuce, and penis were within normal limits. Ultrasonography revealed diffuse hyperechoic areas and were more pronounced in the right tes- tis (Figure 2A) affecting 20% of the examined area. Collection of semen by electroejaculation resulted in 5 ml of ejaculate that had poor gross motility and 65% individual motility. E/N-stained slides had 98.5% morphologically normal sperm (2% with head defects and 1% with midpiece defects). Wet mount had 93% morphologically normal sperm (4% with sperm head defects, 1% with midpiece defects, and 2% with principal piece defects). Necropsy: Left testis had focal white streaks of 5 mm2 in the parenchyma and small areas of hard consistency and granulo- matous appearance. Right testis had a large lesion of hard con- sistency and granulomatous appearance from the mediastinum toward the periphery involving 80% of testis (Figure 2B). Testicular parenchyma had abnormal appearance and atypical color. Epididymis, vas deferens, ampullae, and vesicular glands were within normal limits. In histology, intraluminal dystrophic calcification and peritubular fibrosis of the epidid- ymis, fibrous tissue and calcification with mononuclear and polymorphonuclear cells (Figure 2C) were observed. The diagnosis was severe stromal and intratubular fibrosis. Nested multiplex RT-PCR was negative for NS5B gene of BVD virus identified as BVDV-1 (pestivirus A). Bull C BSE: Scrotum had a distinct neck; testes were symmetrical and were not freely moveable. The tone was abnormal with palpable intratesticular firm swelling nodules in right testis. Left epididymis tail was adhered to the testis. Right epididy- mis and spermatic cords were within normal limits. SC was 33.5 cm, and the scrotal temperature was normal without painful responses. Internal reproductive organs, prepuce, and penis were within normal limits. Ultrasonography revealed large diffuse and focal hyperechoic areas (Figure 3A) in testes affecting ~ 30% of examined areas. Collection of semen by electroejaculation resulted in 5 ml of ejaculate that had very good sperm gross motility and 65% individual motility. E/N-stained slides had 93.5% morphologically nor- mal sperm (13% sperm head defects). The percentage of morphologically normal sperm in wet mount was 92% (3% defects of sperm head, 1% with proximal droplets, and 4% with principal piece defects). Necropsy: There were adhesions between the parietal and visceral vaginal tunics. Testes had large white focal and dif- fuse streaks from the mediastinum toward the periphery of  the parenchyma with lack of bulging (Figure 3B). Epididymides had adhesions to the vaginal tunic; vas deferens, ampullae, and vesicular glands were within nor- mal limits. Testes histopathology revealed 70% mineraliza- tion of the seminiferous tubules with active fibroblasts and inflammatory cells (lymphocytes and macrophages) in the interstitial compartment. Seminiferous tubules had abnor- mal epithelium and intratubular concentric laminated cal- cification. There was a reduction in germinal cells; germinal cells were missing entirely in some tubules (Figure 3C). The diagnosis was severe testicular degeneration with moderate interstitial fibroplasia including areas with necrosis. Nested multiplex RT-PCR was negative for NS5B gene of BVD virus identified as BVDV-1 (pestivirus A). Figure 2. Bull B: A. Ultrasonogram of right testis; note large hyperechoic areas (black arrows). B. Cut section of testes; note the hard consistency and granulomatous appearance of parenchyma (compatible with fibrosis) with severe lesions in right testis (black arrows). C. Light microscopic image (400 x) of testis; note mineralized seminiferous tubules with reduction on germinal cells (black arrows) http://dx.doi.org/10.58292/CT.v17.12711 4 Citation: Clinical Theriogenology 2025, 17, 12711, http://dx.doi.org/10.58292/CT.v17.12711 Bull D BSE: Scrotum had a distinct neck; testes were symmetrical with normal tone, and they were freely moveable. Epididymis and spermatic cords were within normal limits. SC was 38.0 cm, and the scrotal temperature was normal without painful responses. Internal reproductive organs, prepuce, and penis were within normal limits. Ultrasonography of testes revealed small focal hyperechoic areas affecting < 1% of the examined area. Collection of semen by electroejaculation resulted in 9 ml of ejaculate with very good gross motility and 90% indi- vidual motility. E/N-stained slides had 99% morphologically normal sperm (1% defects of sperm head). The percentage of morphologically normal sperm cells in wet mount was 97% (2% of detached normal sperm heads, 1% simple bent tail). Discussion There were severe testicular lesions in these 3 bulls. Bull A had stromal fibrosis in the left testis and NS5B gene was detected by RT-PCR suggesting an infection with BVDV-1 (pestivirus A). Bulls B and C were suspected of testicular fibrosis on palpation and ultrasonography revealed abnor- mal hyperechoic areas. They also had low SC; macroscopic signs of degeneration, fibrosis, and mineralization that were confirmed by histopathology. Testicular degeneration, fibrosis, and mineralization have been described in bulls with reduced fertility; causes suggested were biopsy, trauma, heat, cold, radiation, nutritional disor- ders, toxic agents, blind efferent ductules, vascular lesions, and aging.9 Testicular biopsy could result in hemorrhage, infarc- tion, and degeneration9; however, in this case, no signs of pre- vious biopsy were observed, and the lesions were in the mediastinum and toward the periphery. Testicular trauma is rare in bulls,9 and in these cases, classical hematomas and necrotic lesions were not observed. Blind efferent ductules or obstruction at the level of epididymis were suspected, espe- cially in bull A that did not respond to electroejaculation. Lesions on the hemato-testicular barrier with spermiostasis often cause testicular degeneration, fibrosis, and mineraliza- tion.9 However, duct permeability was normal in that bull and no macroscopic and microscopic signs of obstructive lesions were observed in any bulls. Infectious agents could cause inflammation of the arterioles and capillaries in the testes inducing necrosis and fibrosis.10,11 In fact, there is evidence that bovine respiratory syncytial virus could be involved in the etiology of testicular fibrosis.11 BVD virus can be detected in the testes for a long duration after acute infection.12 Mass motility, sperm concentration, and herd fertility were signifi- cantly lower in serologically BVD-positive bulls.13 However, although BVD virus was detected in a testicular sample of bull A, the role of the virus on infertility cannot be determined. Testicular degeneration is an acquired condition that results in testes softer than normal on palpation, low concentration of sperm, with a high percentage of morphological defects.4 In the present case, the specific cause of testicular degeneration, Figure 3. Bull C: A. Ultrasonogram of testis; note large hyperechoic areas (black arrows). B. Cut section of testes; note severe fibro- sis from the rete toward the peripheral parenchyma (black arrows) with lack of bulging (white arrows). C. Light microscopic image (400 x) of testis; note seminiferous tubules with abnormal epithelium and intratubular concentric laminated calcification (black arrows) http://dx.doi.org/10.58292/CT.v17.12711 Citation: Clinical Theriogenology 2025, 17, 12711, http://dx.doi.org/10.58292/CT.v17.12711 5 fibrosis, and mineralization could not be confirmed; however, it could have been the cause of low pregnancy rate in this group of heifers. Testicular degeneration can decrease SC and it may be temporary if the underlying cause is removed.11 The SC provides a reliable estimate of testicular mass and is useful in estimating daily sperm production and semen quality.4 Severe testicular degeneration was diagnosed in bull C and the SC was below the normal range. However, semen parameters were within normal range for all 3 bulls. Probably, there was enough healthy testicular tissue to produce a fair amount of normal semen. The lack of sperm at electroejaculation in bull A was probably due to lack of response to the technique. Occasionally, it is difficult to collect from bulls that have been active with cows in the last 24 hours or nervous, irritable, or hard to manage.14 Although macroscopic lesions in the testes detected by ultra- sonography have been associated with low semen quality and an increase in sperm abnormalities,15 others reported that the degree of testis fibrosis was not associated with semen qual- ity,11 and mild lesions would not affect testicular function or sperm quality.16 In the present case, the extension and severity of testicular degeneration, fibrosis, and mineralization proba- bly reduced the amount of testicular tissue producing sperm and consequently reduced fertility. Learning points • Breeding soundness examination of bulls before breeding season is reaffirmed • Ultrasonography of testes is of great value • Limangus breeders should be alerted to detect early testic- ular problems Conflict of interest None to declare. References 1. 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