1 CONTACT Rebecca Funk rafunk@vt.edu © 2023 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http:// creativecommons.org/licenses/by-nc/4.0/), permitting all noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Citation line: Clinical Theriogenology 2023, 15, 9395, http://dx.doi.org/10.58292/CT.v15.9395 Case Report Double cervices in a Gypsy Vanner mare Joanna Kania,a Katie Wilson,a Terje Raudsepp,b Caitlin Castaneda,b Matthew Jevit,b Maria Horteloup,c Nadia Saklou,a Catherine Jula,a Rebecca Funka aDepartment of Large Animal Clinical Sciences, Virginia College of Veterinary Medicine, Blacksburg, VA, USA; bDepartment of Veterinary Integrative Biosciences, Texas A&M University, School of Veterinary Medicine and Biosciences, College Station, TX, USA; cNUTREP, LLC, Blacksburg, VA, USA Abstract A 7-year, purebred Gypsy Vanner, maiden mare was presented with abnormal cervical tissue that was detected during prebreeding examination. External vulvar examination, transrectal and ultrasonographic examination of uterus, and internal vaginal vault and cervical examination (via speculum and endoscopy) were conducted. Karyotype analysis included sex chromosome study with dual-color fluorescence in situ hybridization and polymerase chain reaction for SRY gene. Mare had normal 64,XX karyotype with no evidence of mosaicism. Two distinct, patent, cervical ora separated by a frenulum, leading to a single uterine body, were observed. It was recommended to avoid breeding because of dystocia risk. To the authors’ knowledge, this is the first reported case of this nature in a mare. Keywords: Mare, reproductive anomaly, double cervices, fluorescence in situ hybridization, karyotyping Background Any deviation from normal sexual development, whether chromosomal, gonadal, or anatomical, is referred to as disorders in sexual development (DSD).1 In horses, DSDs are understudied but not uncommon and clinical manifesta- tions are often associated with reproductive problems. Normal development of cervix and uterus rely on appropri- ate differentiation of Müllerian duct and urogenital sinus. In people, abnormalities of Müllerian duct formation resulted in congenital uterine and cervical aberrations.2 Congenital uterine and cervical abnormalities in mares were observed in draft breeds; however, breed associations of DSDs were not fully investigated.3–5 Although these structural abnormalities are not necessarily associated with infertility, chromosome analysis is useful in exploring fertility complications. Compromised fertility was noted in an Irish cob mare with congenital cervical abnormality and concurrent chromo- somal aberration (mosaic aneuploidy of the X chromo- some).5 This case report describes double cervices in a 7-year mare with no other reproductive tract, chromosomal, or karyotype abnormalities. Case presentation A 7-year, Gypsy Vanner (also known as Irish Cob) maiden mare was referred for investigation of abnormal cervical anatomy detected during a prebreeding evaluation. Mare had a history of recurrent colic but was otherwise apparently healthy. Complete reproductive evaluation was warranted because the mare was intended for breeding. On presentation, mare was bright and alert. Her external gen- italia had upright vulvar orientation, ~ 80% vulvar opening was below pelvic brim with appropriate vulvar and vulvo-vag- inal seals. Transrectal palpation revealed symmetrical uterine horns and ovaries of adequate size, shape, tone, and texture for a cyclic, maiden, draft mare. Transrectal ultrasonography revealed normal uterus with no cysts, fluid, or edema. Mare had 1 uterine body with 2 distinct uterine horns visualized from the bifurcation of the uterus. Both ovaries had a corpus luteum. Two firm, taught cervices were felt and could be fol- lowed to caudal uterine body (Figure 1). Mare had adequate vulvo-vaginal seal when speculum was inserted. Moderate amount of thick and white mucus was pres- ent on vaginal vault’s ventral aspect. Two patent, cervical ora were identified at the cranial surface of the vault that had nor- mal tone, size, and structure. A thin (~ 2 cm) frenulum was noticed between the 2 cervices. A sterile endoscope was initially inserted through the right cervix and her uterus was insufflated and visualized (Figure 2). There was scant amount of thick and white mucus in the uterus. Uterine lining, both uterine horns, mailto:rafunk@vt.edu http://creativecommons.org/licenses/by-nc/4.0/ http://creativecommons.org/licenses/by-nc/4.0/ http://dx.doi.org/10.58292/CT.v15.9395 2 Citation line: Clinical Theriogenology 2023, 15, 9395, http://dx.doi.org/10.58292/CT.v15.9395 oviductal papillae, and uterine bifurcation were visualized in their entirety and were unremarkable. Endoscope was removed from the right cervix and passed through the left cervical os into uterine body. Scope was retroflexed to obtain an image of both cervical openings within uterine body lumen. Blood sample was collected for short-term pokeweed-stimu- lated lymphocyte culture. Thirty metaphase cells were analyzed; seven were karyotyped that identified a 64,XX female karyotype with no observed chromosomal abnormalities. Polymerase chain reaction (PCR) for the Y-linked male sex determination gene SRY was negative, consistent with XX sex chromosomes. To verify nonmosaic 64,XX karyotype, dual-color fluorescence in situ hybridization (FISH) was conducted with a combina- tion of 2 probes: flow-sorted horse X chromosome painting probe and probe specific to equine testis-specific transcript 7 (ETSTY7) ampliconic array that is present in X and Y chromo- somes.6–8 Probes were labeled by nick translation (i.e. X paint- ing probe) with biotin using Biotin-Nick Translation Mix and ETSTY7 with digoxigenin using DIG-Nick Translation Mix [Roche Diagnostics, Basel, Switzerland], following manufactur- er’s protocol. Hybridization and signal detection followed stan- dard protocols described.8 Biotin-labeled probes were detected with Alexa Fluor® 488 streptavidin conjugate (Molecular Figure 1. Ultrasonogram of caudal reproductive tract (note 2 analogous cervices [arrows]). Figure 2. Endoscopic images of two cervicies (A), frenelum between 2 cervices (B), uterine body lumen (C) http://dx.doi.org/10.58292/CT.v15.9395 Citation line: Clinical Theriogenology 2023, 15, 9395, http://dx.doi.org/10.58292/CT.v15.9395 3 Probes, Life Technologies, Carlsbad, CA) and digoxigenin-la- beled probes with DyLight®594 antidigoxigenin  conjugate (Vector Laboratories, Burlingame, CA). Chromosomes were counterstained with 4’,6-diamidino-2-phenylindole. One hundred cells were analyzed using a motorized fluorescence microscope (Axio Imager M2p [Zeiss]) equipped with a high-resolution progressive scan CCD camera CoolCube 1 and Isis v5.3.18 software (MetaSystems GmbH, Altlußheim, Germany). FISH with probes specific for equine sex chromo- somes (Figure 3) was consistent with the results of karyotyping (all 100 cells had 64,XX karyotype). There was no evidence for mosaicism for XY cells or cells with a single X chromosome. Mare was diagnosed with an isolated DSD, namely double cer- vical ora without other concurrent genetic, structural, or repro- ductive abnormalities. No further diagnostics or interventions were performed and the mare was discharged with recommen- dations against carrying a foal due to risk for dystocia. Discussion Dual cervical ora has been previously reported in a maiden Gypsy Vanner mare, with concurrent uterine didelphys and chromosomal abnormalities.5 Although complete uterine didelphys (2 separate reproductive tracts from vaginal vault to uterine horn) is a normal anatomy in several mammals (e.g., marsupials and rodents),9,10 in the mare 1 vaginal vault and cervix leading to bicornate uterine body is the norm. Reproductive tract abnormalities were documented in sev- eral species due to partial or full failure of fusion of the Müllerian ducts.10 Abnormalities documented in normally monodelphic (1 reproductive tract) animals were bands of tissue remaining within the cervical os and/or canal, double external cervical os joining into 1 cervical canal, or, rarely 2 external cervices each leading to a uterine horn separated by a complete septum (true didelphys).11 Rarely, 2 complete and separate cervical canals opening into 1, structurally nor- mal, uterus were observed. Brown throated sloth (Bradypus variegatus) has a simplex uterus with 2 complete cervical canals (considered normal anatomy).12 A case of rare DSD (cervical duplication) in the absence of chromosomal aberrations is documented. This was con- firmed by an initial cytogenetic analysis of 30 cells, followed by FISH analysis of sex chromosomes in 100 cells, ruling out low-level mosaicism for a chromosomally aberrant cell line. Causative factor(s) and incidence of cervical  anomalies in horses have not been identified. Documentation of 2 mares of the same breed with DSD should warrant further explora- tion into a possible breed disposition for these conditions.5 There was no evidence of infertility in this patient; however, parturition complications are possible. Although there is limited information on parturition complications in patients with isolated double cervices, there have been stud- ies that explored parturition complications in patients with vaginal septa. Vaginal dystocia was common in these patients and often the reason for discovering the anomaly.13,14 Although double cervices are different from vaginal septa, they both cause an obstruction of the egress from the uterus. During embryogenesis, SRY gene differentiates gonads into testes. Typically, this gene is on Y chromosome; however, in some cases in people it was on X chromosomes that was asso- ciated with DSDs.15 Since this mare was SRY-negative, an SRY anomaly was not responsible for DSD. This case report highlighted the importance of thorough examination of potential breeding animals including vagi- noscopy and hysteroscopy. Karyotype analysis is not com- monly performed; however, it can be helpful in identifying potential DSD. As demonstrated in this case, some forms of DSD are chromosomally normal, suggesting possible involvement of submicroscopic genetic changes. In addition, exposure to some endocrine-disrupting agents altered uter- ine development in other species.16 The broad range of pos- sible disruptions to normal sexual development in horses is not as widely documented as. in other species, nor are they fully understood. Further study of causative factors in horses will increase understanding and better handling of unusual DSD cases. Learning points • Double cervices can occur without other reproductive tract, chromosomal, or karyotype abnormalities • Breeding animals should be examined throuughly includ- ing, if possible, endoscopy and hysterscopy examinations Conflict of interest None to report. References 1. Allen L: Disorders of sexual development. Obstet Gynecol Clin North Am 2009;36:24–45. doi: 10.1016/j.ogc.2009.02.001 2. Golan A, Langer R, Bukovsky I, et al: Congenital anomalies of the Müllerian system. Fertil Steril 1989;51:747–755. doi: 10.1016/ S0015-0282(16)60660-X Figure 3. 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