id	sid	tid	token	lemma	pos
cvj-49	1	1	type	type	NOUN
cvj-49	1	2	of	of	ADP
cvj-49	1	3	the	the	DET
cvj-49	1	4	paper	paper	NOUN
cvj-49	1	5	(	(	PUNCT
cvj-49	1	6	article	article	NOUN
cvj-49	1	7	cluj	cluj	PROPN
cvj-49	1	8	vet	vet	PROPN
cvj-49	1	9	j	j	PROPN
cvj-49	1	10	2023	2023	NUM
cvj-49	1	11	vol	vol	NOUN
cvj-49	1	12	28	28	NUM
cvj-49	1	13	,	,	PUNCT
cvj-49	1	14	issue	issue	NOUN
cvj-49	1	15	2	2	NUM
cvj-49	1	16	http://clujveterinaryjournal.ro	http://clujveterinaryjournal.ro	PROPN
cvj-49	1	17	review	review	NOUN
cvj-49	1	18	flow	flow	NOUN
cvj-49	1	19	cytometry	cytometry	NOUN
cvj-49	1	20	and	and	CCONJ
cvj-49	1	21	its	its	PRON
cvj-49	1	22	use	use	NOUN
cvj-49	1	23	in	in	ADP
cvj-49	1	24	modern	modern	ADJ
cvj-49	1	25	human	human	ADJ
cvj-49	1	26	and	and	CCONJ
cvj-49	1	27	veterinary	veterinary	ADJ
cvj-49	1	28	andrology	andrology	NOUN
cvj-49	1	29	diana	diana	PROPN
cvj-49	1	30	cenariu	cenariu	VERB
cvj-49	1	31	1	1	NUM
cvj-49	1	32	,	,	PUNCT
cvj-49	1	33	*	*	PUNCT
cvj-49	1	34	,	,	PUNCT
cvj-49	1	35	jon	jon	PROPN
cvj-49	1	36	thor	thor	PROPN
cvj-49	1	37	bergthorsson2	bergthorsson2	PROPN
cvj-49	1	38	,	,	PUNCT
cvj-49	1	39	victor	victor	PROPN
cvj-49	1	40	greiff3	greiff3	PROPN
cvj-49	1	41	,	,	PUNCT
cvj-49	1	42	bogdanțigu1	bogdanțigu1	PROPN
cvj-49	1	43	,	,	PUNCT
cvj-49	1	44	valentin	valentin	X
cvj-49	1	45	toma1	toma1	NOUN
cvj-49	1	46	and	and	CCONJ
cvj-49	1	47	mihai	mihai	PROPN
cvj-49	1	48	cenariu	cenariu	PROPN
cvj-49	1	49	4	4	NUM
cvj-49	1	50	1	1	NUM
cvj-49	1	51	research	research	NOUN
cvj-49	1	52	center	center	NOUN
cvj-49	1	53	for	for	ADP
cvj-49	1	54	advanced	advanced	ADJ
cvj-49	1	55	medicine	medicine	NOUN
cvj-49	1	56	–	–	PUNCT
cvj-49	1	57	medfuture	medfuture	NOUN
cvj-49	1	58	,	,	PUNCT
cvj-49	1	59	iuliu	iuliu	PROPN
cvj-49	1	60	hatieganu	hatieganu	PROPN
cvj-49	1	61	university	university	PROPN
cvj-49	1	62	of	of	ADP
cvj-49	1	63	medicine	medicine	NOUN
cvj-49	1	64	and	and	CCONJ
cvj-49	1	65	pharmacy	pharmacy	NOUN
cvj-49	1	66	cluj	cluj	NOUN
cvj-49	1	67	-	-	PUNCT
cvj-49	1	68	napoca	napoca	PROPN
cvj-49	1	69	,	,	PUNCT
cvj-49	1	70	romania	romania	PROPN
cvj-49	1	71	;	;	PUNCT
cvj-49	2	1	diacenariu@gmail.com	diacenariu@gmail.com	X
cvj-49	2	2	2	2	NUM
cvj-49	2	3	university	university	NOUN
cvj-49	2	4	of	of	ADP
cvj-49	2	5	iceland	iceland	PROPN
cvj-49	2	6	,	,	PUNCT
cvj-49	2	7	reykjavik	reykjavik	PROPN
cvj-49	2	8	,	,	PUNCT
cvj-49	2	9	iceland	iceland	PROPN
cvj-49	2	10	;	;	PUNCT
cvj-49	2	11	jon.bergthorsson@gmail.com	jon.bergthorsson@gmail.com	PROPN
cvj-49	2	12	3	3	NUM
cvj-49	2	13	university	university	PROPN
cvj-49	2	14	of	of	ADP
cvj-49	2	15	oslo	oslo	PROPN
cvj-49	2	16	,	,	PUNCT
cvj-49	2	17	norway	norway	PROPN
cvj-49	2	18	;	;	PUNCT
cvj-49	2	19	victor.greiff@medisin.uio.no	victor.greiff@medisin.uio.no	PROPN
cvj-49	2	20	4	4	NUM
cvj-49	2	21	university	university	NOUN
cvj-49	2	22	of	of	ADP
cvj-49	2	23	agricultural	agricultural	ADJ
cvj-49	2	24	sciences	science	NOUN
cvj-49	2	25	and	and	CCONJ
cvj-49	2	26	veterinary	veterinary	ADJ
cvj-49	2	27	medicine	medicine	NOUN
cvj-49	2	28	cluj	cluj	PROPN
cvj-49	2	29	-	-	PUNCT
cvj-49	2	30	napoca	napoca	PROPN
cvj-49	2	31	,	,	PUNCT
cvj-49	2	32	romania	romania	PROPN
cvj-49	2	33	;	;	PUNCT
cvj-49	2	34	mcenariu@yahoo.es	mcenariu@yahoo.es	PROPN
cvj-49	2	35	*	*	PUNCT
cvj-49	2	36	correspondence	correspondence	NOUN
cvj-49	2	37	:	:	PUNCT
cvj-49	3	1	diacenariu@gmail.com	diacenariu@gmail.com	X
cvj-49	3	2	;	;	PUNCT
cvj-49	3	3	abstract	abstract	ADJ
cvj-49	3	4	:	:	PUNCT
cvj-49	3	5	fertility	fertility	NOUN
cvj-49	3	6	of	of	ADP
cvj-49	3	7	a	a	DET
cvj-49	3	8	male	male	NOUN
cvj-49	3	9	is	be	AUX
cvj-49	3	10	sometimes	sometimes	ADV
cvj-49	3	11	difficult	difficult	ADJ
cvj-49	3	12	to	to	PART
cvj-49	3	13	assess	assess	VERB
cvj-49	3	14	and	and	CCONJ
cvj-49	3	15	results	result	NOUN
cvj-49	3	16	obtained	obtain	VERB
cvj-49	3	17	using	use	VERB
cvj-49	3	18	regular	regular	ADJ
cvj-49	3	19	sperm	sperm	NOUN
cvj-49	3	20	analysis	analysis	NOUN
cvj-49	3	21	methods	method	NOUN
cvj-49	3	22	are	be	AUX
cvj-49	3	23	often	often	ADV
cvj-49	3	24	inconclusive	inconclusive	ADJ
cvj-49	3	25	.	.	PUNCT
cvj-49	4	1	flow	flow	NOUN
cvj-49	4	2	cytometry	cytometry	NOUN
cvj-49	4	3	was	be	AUX
cvj-49	4	4	proven	prove	VERB
cvj-49	4	5	to	to	PART
cvj-49	4	6	generate	generate	VERB
cvj-49	4	7	crucial	crucial	ADJ
cvj-49	4	8	information	information	NOUN
cvj-49	4	9	,	,	PUNCT
cvj-49	4	10	that	that	PRON
cvj-49	4	11	allows	allow	VERB
cvj-49	4	12	specialists	specialist	NOUN
cvj-49	4	13	to	to	PART
cvj-49	4	14	conclude	conclude	VERB
cvj-49	4	15	upon	upon	SCONJ
cvj-49	4	16	the	the	DET
cvj-49	4	17	reproductive	reproductive	ADJ
cvj-49	4	18	capacity	capacity	NOUN
cvj-49	4	19	of	of	ADP
cvj-49	4	20	an	an	DET
cvj-49	4	21	individual	individual	NOUN
cvj-49	4	22	.	.	PUNCT
cvj-49	5	1	together	together	ADV
cvj-49	5	2	with	with	ADP
cvj-49	5	3	computer	computer	NOUN
cvj-49	5	4	assisted	assist	VERB
cvj-49	5	5	sperm	sperm	NOUN
cvj-49	5	6	analysis	analysis	NOUN
cvj-49	5	7	casa	casa	PROPN
cvj-49	5	8	systems	system	NOUN
cvj-49	5	9	,	,	PUNCT
cvj-49	5	10	flow	flow	NOUN
cvj-49	5	11	cytometers	cytometer	NOUN
cvj-49	5	12	allow	allow	VERB
cvj-49	5	13	an	an	DET
cvj-49	5	14	in	in	ADP
cvj-49	5	15	-	-	PUNCT
cvj-49	5	16	depth	depth	NOUN
cvj-49	5	17	analysis	analysis	NOUN
cvj-49	5	18	of	of	ADP
cvj-49	5	19	fresh	fresh	ADJ
cvj-49	5	20	,	,	PUNCT
cvj-49	5	21	chilled	chilled	ADJ
cvj-49	5	22	or	or	CCONJ
cvj-49	5	23	frozen	frozen	ADJ
cvj-49	5	24	/	/	SYM
cvj-49	5	25	thawed	thaw	VERB
cvj-49	5	26	semen	semen	NOUN
cvj-49	5	27	,	,	PUNCT
cvj-49	5	28	which	which	PRON
cvj-49	5	29	is	be	AUX
cvj-49	5	30	currently	currently	ADV
cvj-49	5	31	essential	essential	ADJ
cvj-49	5	32	for	for	ADP
cvj-49	5	33	research	research	NOUN
cvj-49	5	34	purposes	purpose	NOUN
cvj-49	5	35	,	,	PUNCT
cvj-49	5	36	but	but	CCONJ
cvj-49	5	37	also	also	ADV
cvj-49	5	38	for	for	ADP
cvj-49	5	39	diagnosis	diagnosis	NOUN
cvj-49	5	40	of	of	ADP
cvj-49	5	41	various	various	ADJ
cvj-49	5	42	male	male	NOUN
cvj-49	5	43	-	-	PUNCT
cvj-49	5	44	related	relate	VERB
cvj-49	5	45	fertility	fertility	NOUN
cvj-49	5	46	disorders	disorder	NOUN
cvj-49	5	47	,	,	PUNCT
cvj-49	5	48	both	both	CCONJ
cvj-49	5	49	in	in	ADP
cvj-49	5	50	veterinary	veterinary	ADJ
cvj-49	5	51	and	and	CCONJ
cvj-49	5	52	human	human	ADJ
cvj-49	5	53	medicine	medicine	NOUN
cvj-49	5	54	.	.	PUNCT
cvj-49	6	1	several	several	ADJ
cvj-49	6	2	parameters	parameter	NOUN
cvj-49	6	3	can	can	AUX
cvj-49	6	4	be	be	AUX
cvj-49	6	5	assessed	assess	VERB
cvj-49	6	6	using	use	VERB
cvj-49	6	7	this	this	DET
cvj-49	6	8	method	method	NOUN
cvj-49	6	9	,	,	PUNCT
cvj-49	6	10	such	such	ADJ
cvj-49	6	11	as	as	ADP
cvj-49	6	12	sperm	sperm	NOUN
cvj-49	6	13	viability	viability	NOUN
cvj-49	6	14	,	,	PUNCT
cvj-49	6	15	acrosome	acrosome	NOUN
cvj-49	6	16	integrity	integrity	NOUN
cvj-49	6	17	,	,	PUNCT
cvj-49	6	18	mitochondrial	mitochondrial	ADJ
cvj-49	6	19	activity	activity	NOUN
cvj-49	6	20	,	,	PUNCT
cvj-49	6	21	concentration	concentration	NOUN
cvj-49	6	22	and	and	CCONJ
cvj-49	6	23	total	total	ADJ
cvj-49	6	24	sperm	sperm	NOUN
cvj-49	6	25	number	number	NOUN
cvj-49	6	26	,	,	PUNCT
cvj-49	6	27	dna	dna	PROPN
cvj-49	6	28	fragmentation	fragmentation	NOUN
cvj-49	6	29	,	,	PUNCT
cvj-49	6	30	capacitation	capacitation	NOUN
cvj-49	6	31	,	,	PUNCT
cvj-49	6	32	oxidative	oxidative	ADJ
cvj-49	6	33	stress	stress	NOUN
cvj-49	6	34	,	,	PUNCT
cvj-49	6	35	etc	etc	X
cvj-49	6	36	.	.	X
cvj-49	7	1	this	this	DET
cvj-49	7	2	paper	paper	NOUN
cvj-49	7	3	provides	provide	VERB
cvj-49	7	4	a	a	DET
cvj-49	7	5	rapid	rapid	ADJ
cvj-49	7	6	reference	reference	NOUN
cvj-49	7	7	to	to	ADP
cvj-49	7	8	specialists	specialist	NOUN
cvj-49	7	9	involved	involve	VERB
cvj-49	7	10	in	in	ADP
cvj-49	7	11	semen	semen	NOUN
cvj-49	7	12	analysis	analysis	NOUN
cvj-49	7	13	by	by	ADP
cvj-49	7	14	flow	flow	NOUN
cvj-49	7	15	cytometry	cytometry	NOUN
cvj-49	7	16	,	,	PUNCT
cvj-49	7	17	regarding	regard	VERB
cvj-49	7	18	semen	semen	NOUN
cvj-49	7	19	sample	sample	NOUN
cvj-49	7	20	preparation	preparation	NOUN
cvj-49	7	21	,	,	PUNCT
cvj-49	7	22	instrument	instrument	NOUN
cvj-49	7	23	setup	setup	NOUN
cvj-49	7	24	,	,	PUNCT
cvj-49	7	25	and	and	CCONJ
cvj-49	7	26	evaluation	evaluation	NOUN
cvj-49	7	27	of	of	ADP
cvj-49	7	28	the	the	DET
cvj-49	7	29	most	most	ADV
cvj-49	7	30	important	important	ADJ
cvj-49	7	31	sperm	sperm	NOUN
cvj-49	7	32	parameters	parameter	NOUN
cvj-49	7	33	(	(	PUNCT
cvj-49	7	34	viability	viability	NOUN
cvj-49	7	35	,	,	PUNCT
cvj-49	7	36	acrosome	acrosome	NOUN
cvj-49	7	37	reaction	reaction	NOUN
cvj-49	7	38	and	and	CCONJ
cvj-49	7	39	mitochondrial	mitochondrial	ADJ
cvj-49	7	40	activity	activity	NOUN
cvj-49	7	41	)	)	PUNCT
cvj-49	7	42	.	.	PUNCT
cvj-49	8	1	keywords	keyword	NOUN
cvj-49	8	2	:	:	PUNCT
cvj-49	8	3	flow	flow	NOUN
cvj-49	8	4	cytometry	cytometry	NOUN
cvj-49	8	5	;	;	PUNCT
cvj-49	8	6	mammalian	mammalian	ADJ
cvj-49	8	7	sperm	sperm	NOUN
cvj-49	8	8	;	;	PUNCT
cvj-49	8	9	viability	viability	NOUN
cvj-49	8	10	;	;	PUNCT
cvj-49	8	11	acrosome	acrosome	NOUN
cvj-49	8	12	integrity	integrity	NOUN
cvj-49	8	13	;	;	PUNCT
cvj-49	8	14	mitochondrial	mitochondrial	ADJ
cvj-49	8	15	activity	activity	NOUN
cvj-49	8	16	.	.	PUNCT
cvj-49	9	1	1	1	X
cvj-49	9	2	.	.	X
cvj-49	9	3	introduction	introduction	NOUN
cvj-49	9	4	flow	flow	NOUN
cvj-49	9	5	cytometers	cytometer	NOUN
cvj-49	9	6	are	be	AUX
cvj-49	9	7	made	make	VERB
cvj-49	9	8	up	up	ADP
cvj-49	9	9	of	of	ADP
cvj-49	9	10	several	several	ADJ
cvj-49	9	11	independent	independent	ADJ
cvj-49	9	12	systems	system	NOUN
cvj-49	9	13	that	that	PRON
cvj-49	9	14	are	be	AUX
cvj-49	9	15	interconnected	interconnect	VERB
cvj-49	9	16	to	to	PART
cvj-49	9	17	yield	yield	VERB
cvj-49	9	18	the	the	DET
cvj-49	9	19	final	final	ADJ
cvj-49	9	20	results	result	NOUN
cvj-49	9	21	,	,	PUNCT
cvj-49	9	22	such	such	ADJ
cvj-49	9	23	as	as	ADP
cvj-49	9	24	fluidics	fluidic	NOUN
cvj-49	9	25	,	,	PUNCT
cvj-49	9	26	that	that	PRON
cvj-49	9	27	allows	allow	VERB
cvj-49	9	28	a	a	DET
cvj-49	9	29	single	single	ADJ
cvj-49	9	30	-	-	PUNCT
cvj-49	9	31	stranded	strand	VERB
cvj-49	9	32	alignment	alignment	NOUN
cvj-49	9	33	of	of	ADP
cvj-49	9	34	events	event	NOUN
cvj-49	9	35	,	,	PUNCT
cvj-49	9	36	optics	optic	NOUN
cvj-49	9	37	,	,	PUNCT
cvj-49	9	38	made	make	VERB
cvj-49	9	39	up	up	ADP
cvj-49	9	40	of	of	ADP
cvj-49	9	41	several	several	ADJ
cvj-49	9	42	lasers	laser	NOUN
cvj-49	9	43	,	,	PUNCT
cvj-49	9	44	light	light	ADJ
cvj-49	9	45	filters	filter	NOUN
cvj-49	9	46	and	and	CCONJ
cvj-49	9	47	detectors	detector	NOUN
cvj-49	9	48	as	as	ADV
cvj-49	9	49	well	well	ADV
cvj-49	9	50	as	as	ADP
cvj-49	9	51	electronics	electronic	NOUN
cvj-49	9	52	,	,	PUNCT
cvj-49	9	53	which	which	PRON
cvj-49	9	54	allows	allow	VERB
cvj-49	9	55	conversion	conversion	NOUN
cvj-49	9	56	of	of	ADP
cvj-49	9	57	optical	optical	ADJ
cvj-49	9	58	signals	signal	NOUN
cvj-49	9	59	into	into	ADP
cvj-49	9	60	electronic	electronic	ADJ
cvj-49	9	61	information	information	NOUN
cvj-49	9	62	which	which	PRON
cvj-49	9	63	can	can	AUX
cvj-49	9	64	be	be	AUX
cvj-49	9	65	stored	store	VERB
cvj-49	9	66	and	and	CCONJ
cvj-49	9	67	analysed	analyse	VERB
cvj-49	9	68	by	by	ADP
cvj-49	9	69	a	a	DET
cvj-49	9	70	computer	computer	NOUN
cvj-49	9	71	,	,	PUNCT
cvj-49	9	72	equipped	equip	VERB
cvj-49	9	73	with	with	ADP
cvj-49	9	74	a	a	DET
cvj-49	9	75	dedicated	dedicated	ADJ
cvj-49	9	76	software	software	NOUN
cvj-49	9	77	.	.	PUNCT
cvj-49	10	1	as	as	ADP
cvj-49	10	2	such	such	ADJ
cvj-49	10	3	,	,	PUNCT
cvj-49	10	4	flow	flow	NOUN
cvj-49	10	5	cytometry	cytometry	NOUN
cvj-49	10	6	represents	represent	VERB
cvj-49	10	7	a	a	DET
cvj-49	10	8	powerful	powerful	ADJ
cvj-49	10	9	technique	technique	NOUN
cvj-49	10	10	that	that	PRON
cvj-49	10	11	allows	allow	VERB
cvj-49	10	12	a	a	DET
cvj-49	10	13	complex	complex	ADJ
cvj-49	10	14	and	and	CCONJ
cvj-49	10	15	prompt	prompt	ADJ
cvj-49	10	16	evaluation	evaluation	NOUN
cvj-49	10	17	,	,	PUNCT
cvj-49	10	18	or	or	CCONJ
cvj-49	10	19	even	even	ADV
cvj-49	10	20	separation	separation	NOUN
cvj-49	10	21	,	,	PUNCT
cvj-49	10	22	of	of	ADP
cvj-49	10	23	single	single	ADJ
cvj-49	10	24	cells	cell	NOUN
cvj-49	10	25	(	(	PUNCT
cvj-49	10	26	called	call	VERB
cvj-49	10	27	events	event	NOUN
cvj-49	10	28	)	)	PUNCT
cvj-49	10	29	found	find	VERB
cvj-49	10	30	in	in	ADP
cvj-49	10	31	suspension	suspension	NOUN
cvj-49	10	32	,	,	PUNCT
cvj-49	10	33	making	make	VERB
cvj-49	10	34	it	it	PRON
cvj-49	10	35	therefore	therefore	ADV
cvj-49	10	36	very	very	ADV
cvj-49	10	37	suitable	suitable	ADJ
cvj-49	10	38	for	for	ADP
cvj-49	10	39	sperm	sperm	NOUN
cvj-49	10	40	analysis	analysis	NOUN
cvj-49	10	41	.	.	PUNCT
cvj-49	11	1	cells	cell	NOUN
cvj-49	11	2	can	can	AUX
cvj-49	11	3	be	be	AUX
cvj-49	11	4	thus	thus	ADV
cvj-49	11	5	evaluated	evaluate	VERB
cvj-49	11	6	regarding	regard	VERB
cvj-49	11	7	their	their	PRON
cvj-49	11	8	size	size	NOUN
cvj-49	11	9	(	(	PUNCT
cvj-49	11	10	forward	forward	ADV
cvj-49	11	11	scatter	scatter	NOUN
cvj-49	11	12	)	)	PUNCT
cvj-49	11	13	,	,	PUNCT
cvj-49	11	14	internal	internal	ADJ
cvj-49	11	15	complexity	complexity	NOUN
cvj-49	11	16	usually	usually	ADV
cvj-49	11	17	given	give	VERB
cvj-49	11	18	by	by	ADP
cvj-49	11	19	their	their	PRON
cvj-49	11	20	granularity	granularity	NOUN
cvj-49	11	21	(	(	PUNCT
cvj-49	11	22	side	side	NOUN
cvj-49	11	23	scatter	scatter	NOUN
cvj-49	11	24	)	)	PUNCT
cvj-49	11	25	as	as	ADV
cvj-49	11	26	well	well	ADV
cvj-49	11	27	as	as	ADP
cvj-49	11	28	fluorescent	fluorescent	ADJ
cvj-49	11	29	intensity	intensity	NOUN
cvj-49	11	30	(	(	PUNCT
cvj-49	11	31	when	when	SCONJ
cvj-49	11	32	stained	stain	VERB
cvj-49	11	33	with	with	ADP
cvj-49	11	34	fluorescent	fluorescent	ADJ
cvj-49	11	35	antibodies	antibody	NOUN
cvj-49	11	36	or	or	CCONJ
cvj-49	11	37	dyes	dye	NOUN
cvj-49	11	38	that	that	PRON
cvj-49	11	39	bind	bind	VERB
cvj-49	11	40	to	to	ADP
cvj-49	11	41	the	the	DET
cvj-49	11	42	nucleus	nucleus	NOUN
cvj-49	11	43	,	,	PUNCT
cvj-49	11	44	cytoplasm	cytoplasm	ADJ
cvj-49	11	45	,	,	PUNCT
cvj-49	11	46	or	or	CCONJ
cvj-49	11	47	membrane	membrane	NOUN
cvj-49	11	48	)	)	PUNCT
cvj-49	12	1	[	[	X
cvj-49	12	2	1	1	NUM
cvj-49	12	3	]	]	PUNCT
cvj-49	12	4	.	.	PUNCT
cvj-49	13	1	since	since	SCONJ
cvj-49	13	2	fertility	fertility	NOUN
cvj-49	13	3	of	of	ADP
cvj-49	13	4	a	a	DET
cvj-49	13	5	male	male	NOUN
cvj-49	13	6	is	be	AUX
cvj-49	13	7	sometimes	sometimes	ADV
cvj-49	13	8	difficult	difficult	ADJ
cvj-49	13	9	to	to	PART
cvj-49	13	10	assess	assess	VERB
cvj-49	13	11	and	and	CCONJ
cvj-49	13	12	results	result	NOUN
cvj-49	13	13	obtained	obtain	VERB
cvj-49	13	14	using	use	VERB
cvj-49	13	15	regular	regular	ADJ
cvj-49	13	16	sperm	sperm	NOUN
cvj-49	13	17	analysis	analysis	NOUN
cvj-49	13	18	methods	method	NOUN
cvj-49	13	19	are	be	AUX
cvj-49	13	20	often	often	ADV
cvj-49	13	21	inconclusive	inconclusive	ADJ
cvj-49	13	22	,	,	PUNCT
cvj-49	13	23	flow	flow	NOUN
cvj-49	13	24	cytometry	cytometry	NOUN
cvj-49	13	25	was	be	AUX
cvj-49	13	26	proven	prove	VERB
cvj-49	13	27	to	to	PART
cvj-49	13	28	generate	generate	VERB
cvj-49	13	29	crucial	crucial	ADJ
cvj-49	13	30	information	information	NOUN
cvj-49	13	31	,	,	PUNCT
cvj-49	13	32	that	that	PRON
cvj-49	13	33	allows	allow	VERB
cvj-49	13	34	specialists	specialist	NOUN
cvj-49	13	35	to	to	PART
cvj-49	13	36	conclude	conclude	VERB
cvj-49	13	37	upon	upon	SCONJ
cvj-49	13	38	the	the	DET
cvj-49	13	39	reproductive	reproductive	ADJ
cvj-49	13	40	capacity	capacity	NOUN
cvj-49	13	41	of	of	ADP
cvj-49	13	42	an	an	DET
cvj-49	13	43	individual	individual	NOUN
cvj-49	13	44	.	.	PUNCT
cvj-49	14	1	several	several	ADJ
cvj-49	14	2	parameters	parameter	NOUN
cvj-49	14	3	can	can	AUX
cvj-49	14	4	be	be	AUX
cvj-49	14	5	assessed	assess	VERB
cvj-49	14	6	using	use	VERB
cvj-49	14	7	this	this	DET
cvj-49	14	8	method	method	NOUN
cvj-49	14	9	,	,	PUNCT
cvj-49	14	10	such	such	ADJ
cvj-49	14	11	as	as	ADP
cvj-49	14	12	sperm	sperm	NOUN
cvj-49	14	13	viability	viability	NOUN
cvj-49	14	14	,	,	PUNCT
cvj-49	14	15	acrosome	acrosome	NOUN
cvj-49	14	16	integrity	integrity	NOUN
cvj-49	14	17	,	,	PUNCT
cvj-49	14	18	mitochondrial	mitochondrial	ADJ
cvj-49	14	19	activity	activity	NOUN
cvj-49	14	20	,	,	PUNCT
cvj-49	14	21	concentration	concentration	NOUN
cvj-49	14	22	and	and	CCONJ
cvj-49	14	23	total	total	ADJ
cvj-49	14	24	sperm	sperm	NOUN
cvj-49	14	25	number	number	NOUN
cvj-49	14	26	,	,	PUNCT
cvj-49	14	27	dna	dna	PROPN
cvj-49	14	28	fragmentation	fragmentation	NOUN
cvj-49	14	29	,	,	PUNCT
cvj-49	14	30	capacitation	capacitation	NOUN
cvj-49	14	31	,	,	PUNCT
cvj-49	14	32	oxidative	oxidative	ADJ
cvj-49	14	33	stress	stress	NOUN
cvj-49	14	34	,	,	PUNCT
cvj-49	14	35	etc	etc	X
cvj-49	14	36	.	.	X
cvj-49	15	1	[	[	X
cvj-49	15	2	2	2	NUM
cvj-49	15	3	]	]	PUNCT
cvj-49	15	4	.	.	PUNCT
cvj-49	16	1	this	this	DET
cvj-49	16	2	paper	paper	NOUN
cvj-49	16	3	is	be	AUX
cvj-49	16	4	aimed	aim	VERB
cvj-49	16	5	at	at	ADP
cvj-49	16	6	providing	provide	VERB
cvj-49	16	7	a	a	DET
cvj-49	16	8	rapid	rapid	ADJ
cvj-49	16	9	reference	reference	NOUN
cvj-49	16	10	to	to	ADP
cvj-49	16	11	specialists	specialist	NOUN
cvj-49	16	12	involved	involve	VERB
cvj-49	16	13	in	in	ADP
cvj-49	16	14	semen	semen	NOUN
cvj-49	16	15	analysis	analysis	NOUN
cvj-49	16	16	by	by	ADP
cvj-49	16	17	flow	flow	NOUN
cvj-49	16	18	cytometry	cytometry	NOUN
cvj-49	16	19	,	,	PUNCT
cvj-49	16	20	regarding	regard	VERB
cvj-49	16	21	semen	semen	NOUN
cvj-49	16	22	sample	sample	NOUN
cvj-49	16	23	preparation	preparation	NOUN
cvj-49	16	24	,	,	PUNCT
cvj-49	16	25	instrument	instrument	NOUN
cvj-49	16	26	setup	setup	NOUN
cvj-49	16	27	,	,	PUNCT
cvj-49	16	28	and	and	CCONJ
cvj-49	16	29	evaluation	evaluation	NOUN
cvj-49	16	30	of	of	ADP
cvj-49	16	31	the	the	DET
cvj-49	16	32	most	most	ADV
cvj-49	16	33	important	important	ADJ
cvj-49	16	34	sperm	sperm	NOUN
cvj-49	16	35	parameters	parameter	NOUN
cvj-49	16	36	(	(	PUNCT
cvj-49	16	37	viability	viability	NOUN
cvj-49	16	38	,	,	PUNCT
cvj-49	16	39	acrosome	acrosome	NOUN
cvj-49	16	40	reaction	reaction	NOUN
cvj-49	16	41	and	and	CCONJ
cvj-49	16	42	mitochondrial	mitochondrial	ADJ
cvj-49	16	43	activity	activity	NOUN
cvj-49	16	44	)	)	PUNCT
cvj-49	16	45	.	.	PUNCT
cvj-49	17	1	received	receive	VERB
cvj-49	17	2	:	:	PUNCT
cvj-49	17	3	03.09.2023	03.09.2023	NUM
cvj-49	17	4	accepted	accept	VERB
cvj-49	17	5	:	:	PUNCT
cvj-49	17	6	17.10.2023	17.10.2023	NUM
cvj-49	17	7	published	publish	VERB
cvj-49	17	8	:	:	PUNCT
cvj-49	17	9	20.10.2023	20.10.2023	NUM
cvj-49	17	10	doi	doi	NOUN
cvj-49	17	11	:	:	PUNCT
cvj-49	17	12	10.52331	10.52331	NUM
cvj-49	17	13	/	/	SYM
cvj-49	17	14	cvj.v28i2.49	cvj.v28i2.49	NOUN
cvj-49	17	15	copyright	copyright	NOUN
cvj-49	17	16	:	:	PUNCT
cvj-49	18	1	©	©	PROPN
cvj-49	18	2	2023	2023	NUM
cvj-49	18	3	by	by	ADP
cvj-49	18	4	the	the	DET
cvj-49	18	5	authors	author	NOUN
cvj-49	18	6	.	.	PUNCT
cvj-49	19	1	submitted	submit	VERB
cvj-49	19	2	for	for	ADP
cvj-49	19	3	possible	possible	ADJ
cvj-49	19	4	open	open	ADJ
cvj-49	19	5	access	access	NOUN
cvj-49	19	6	publication	publication	NOUN
cvj-49	19	7	under	under	ADP
cvj-49	19	8	the	the	DET
cvj-49	19	9	terms	term	NOUN
cvj-49	19	10	and	and	CCONJ
cvj-49	19	11	conditions	condition	NOUN
cvj-49	19	12	of	of	ADP
cvj-49	19	13	the	the	DET
cvj-49	19	14	creative	creative	ADJ
cvj-49	19	15	commons	common	NOUN
cvj-49	19	16	attribution	attribution	NOUN
cvj-49	19	17	(	(	PUNCT
cvj-49	19	18	cc	cc	NOUN
cvj-49	19	19	by	by	ADP
cvj-49	19	20	)	)	PUNCT
cvj-49	19	21	license	license	NOUN
cvj-49	19	22	(	(	PUNCT
cvj-49	19	23	http://creativecommons.org/licenses	http://creativecommons.org/licenses	X
cvj-49	19	24	/by/4.0/	/by/4.0/	PROPN
cvj-49	19	25	)	)	PUNCT
cvj-49	19	26	.	.	PUNCT
cvj-49	20	1	cluj	cluj	PROPN
cvj-49	20	2	vet	vet	PROPN
cvj-49	20	3	j	j	PROPN
cvj-49	20	4	2023	2023	NUM
cvj-49	20	5	,	,	PUNCT
cvj-49	20	6	vol	vol	NOUN
cvj-49	20	7	28	28	NUM
cvj-49	20	8	,	,	PUNCT
cvj-49	20	9	issue	issue	VERB
cvj-49	20	10	45	45	NUM
cvj-49	20	11	2	2	NUM
cvj-49	20	12	.	.	PUNCT
cvj-49	21	1	semen	semen	NOUN
cvj-49	21	2	sample	sample	NOUN
cvj-49	21	3	preparation	preparation	NOUN
cvj-49	21	4	semen	seman	NOUN
cvj-49	21	5	analyzed	analyze	VERB
cvj-49	21	6	by	by	ADP
cvj-49	21	7	flow	flow	NOUN
cvj-49	21	8	cytometry	cytometry	NOUN
cvj-49	21	9	may	may	AUX
cvj-49	21	10	originate	originate	VERB
cvj-49	21	11	from	from	ADP
cvj-49	21	12	fresh	fresh	ADJ
cvj-49	21	13	ejaculates	ejaculate	NOUN
cvj-49	21	14	,	,	PUNCT
cvj-49	21	15	collected	collect	VERB
cvj-49	21	16	by	by	ADP
cvj-49	21	17	any	any	PRON
cvj-49	21	18	of	of	ADP
cvj-49	21	19	the	the	DET
cvj-49	21	20	commonly	commonly	ADV
cvj-49	21	21	used	use	VERB
cvj-49	21	22	methods	method	NOUN
cvj-49	21	23	(	(	PUNCT
cvj-49	21	24	masturbation	masturbation	NOUN
cvj-49	21	25	,	,	PUNCT
cvj-49	21	26	artificial	artificial	ADJ
cvj-49	21	27	vagina	vagina	ADJ
cvj-49	21	28	,	,	PUNCT
cvj-49	21	29	electroejaculation	electroejaculation	NOUN
cvj-49	21	30	,	,	PUNCT
cvj-49	21	31	etc	etc	X
cvj-49	21	32	.	.	X
cvj-49	21	33	)	)	PUNCT
cvj-49	22	1	but	but	CCONJ
cvj-49	22	2	may	may	AUX
cvj-49	22	3	also	also	ADV
cvj-49	22	4	be	be	AUX
cvj-49	22	5	obtained	obtain	VERB
cvj-49	22	6	after	after	ADP
cvj-49	22	7	flushing	flush	VERB
cvj-49	22	8	of	of	ADP
cvj-49	22	9	epididymis	epididymis	NOUN
cvj-49	22	10	(	(	PUNCT
cvj-49	22	11	from	from	ADP
cvj-49	22	12	dead	dead	ADJ
cvj-49	22	13	/	/	SYM
cvj-49	22	14	slaughtered	slaughter	VERB
cvj-49	22	15	animals	animal	NOUN
cvj-49	22	16	or	or	CCONJ
cvj-49	22	17	following	follow	VERB
cvj-49	22	18	castration	castration	NOUN
cvj-49	22	19	)	)	PUNCT
cvj-49	22	20	.	.	PUNCT
cvj-49	23	1	frozen	frozen	ADJ
cvj-49	23	2	semen	semen	NOUN
cvj-49	23	3	samples	sample	NOUN
cvj-49	23	4	may	may	AUX
cvj-49	23	5	also	also	ADV
cvj-49	23	6	be	be	AUX
cvj-49	23	7	investigated	investigate	VERB
cvj-49	23	8	by	by	ADP
cvj-49	23	9	flow	flow	NOUN
cvj-49	23	10	cytometry	cytometry	NOUN
cvj-49	23	11	and	and	CCONJ
cvj-49	23	12	such	such	ADJ
cvj-49	23	13	studies	study	NOUN
cvj-49	23	14	are	be	AUX
cvj-49	23	15	of	of	ADP
cvj-49	23	16	particular	particular	ADJ
cvj-49	23	17	interest	interest	NOUN
cvj-49	23	18	to	to	PART
cvj-49	23	19	assess	assess	VERB
cvj-49	23	20	efficacy	efficacy	NOUN
cvj-49	23	21	of	of	ADP
cvj-49	23	22	the	the	DET
cvj-49	23	23	freezing	freezing	NOUN
cvj-49	23	24	method	method	NOUN
cvj-49	23	25	or	or	CCONJ
cvj-49	23	26	suitability	suitability	NOUN
cvj-49	23	27	of	of	ADP
cvj-49	23	28	the	the	DET
cvj-49	23	29	extender	extender	NOUN
cvj-49	23	30	used	use	VERB
cvj-49	23	31	[	[	PUNCT
cvj-49	23	32	3	3	NUM
cvj-49	23	33	]	]	PUNCT
cvj-49	23	34	.	.	PUNCT
cvj-49	24	1	one	one	NUM
cvj-49	24	2	of	of	ADP
cvj-49	24	3	the	the	DET
cvj-49	24	4	biggest	big	ADJ
cvj-49	24	5	issues	issue	NOUN
cvj-49	24	6	that	that	PRON
cvj-49	24	7	has	have	VERB
cvj-49	24	8	to	to	PART
cvj-49	24	9	be	be	AUX
cvj-49	24	10	taken	take	VERB
cvj-49	24	11	into	into	ADP
cvj-49	24	12	consideration	consideration	NOUN
cvj-49	24	13	is	be	AUX
cvj-49	24	14	interference	interference	NOUN
cvj-49	24	15	between	between	ADP
cvj-49	24	16	components	component	NOUN
cvj-49	24	17	of	of	ADP
cvj-49	24	18	the	the	DET
cvj-49	24	19	seminal	seminal	ADJ
cvj-49	24	20	plasma	plasma	NOUN
cvj-49	24	21	or	or	CCONJ
cvj-49	24	22	extender	extender	NOUN
cvj-49	24	23	and	and	CCONJ
cvj-49	24	24	staining	stain	VERB
cvj-49	24	25	dyes	dye	NOUN
cvj-49	24	26	.	.	PUNCT
cvj-49	25	1	in	in	ADP
cvj-49	25	2	cases	case	NOUN
cvj-49	25	3	where	where	SCONJ
cvj-49	25	4	this	this	PRON
cvj-49	25	5	represents	represent	VERB
cvj-49	25	6	a	a	DET
cvj-49	25	7	serious	serious	ADJ
cvj-49	25	8	concern	concern	NOUN
cvj-49	25	9	,	,	PUNCT
cvj-49	25	10	the	the	DET
cvj-49	25	11	most	most	ADV
cvj-49	25	12	suitable	suitable	ADJ
cvj-49	25	13	method	method	NOUN
cvj-49	25	14	of	of	ADP
cvj-49	25	15	sample	sample	NOUN
cvj-49	25	16	clean	clean	ADJ
cvj-49	25	17	-	-	PUNCT
cvj-49	25	18	up	up	NOUN
cvj-49	25	19	or	or	CCONJ
cvj-49	25	20	washing	washing	NOUN
cvj-49	25	21	has	have	VERB
cvj-49	25	22	to	to	PART
cvj-49	25	23	be	be	AUX
cvj-49	25	24	employed	employ	VERB
cvj-49	25	25	.	.	PUNCT
cvj-49	26	1	usually	usually	ADV
cvj-49	26	2	,	,	PUNCT
cvj-49	26	3	a	a	DET
cvj-49	26	4	density	density	NOUN
cvj-49	26	5	gradient	gradient	NOUN
cvj-49	26	6	centrifugation	centrifugation	NOUN
cvj-49	26	7	or	or	CCONJ
cvj-49	26	8	the	the	DET
cvj-49	26	9	swim	swim	NOUN
cvj-49	26	10	-	-	PUNCT
cvj-49	26	11	up	up	ADP
cvj-49	26	12	technique	technique	NOUN
cvj-49	26	13	is	be	AUX
cvj-49	26	14	adequate	adequate	ADJ
cvj-49	26	15	.	.	PUNCT
cvj-49	27	1	another	another	DET
cvj-49	27	2	significant	significant	ADJ
cvj-49	27	3	problem	problem	NOUN
cvj-49	27	4	is	be	AUX
cvj-49	27	5	related	relate	VERB
cvj-49	27	6	to	to	ADP
cvj-49	27	7	the	the	DET
cvj-49	27	8	debris	debris	NOUN
cvj-49	27	9	which	which	PRON
cvj-49	27	10	is	be	AUX
cvj-49	27	11	often	often	ADV
cvj-49	27	12	present	present	ADJ
cvj-49	27	13	in	in	ADP
cvj-49	27	14	the	the	DET
cvj-49	27	15	sperm	sperm	NOUN
cvj-49	27	16	extenders	extender	NOUN
cvj-49	27	17	,	,	PUNCT
cvj-49	27	18	due	due	ADP
cvj-49	27	19	to	to	ADP
cvj-49	27	20	the	the	DET
cvj-49	27	21	egg	egg	NOUN
cvj-49	27	22	yolk	yolk	NOUN
cvj-49	27	23	or	or	CCONJ
cvj-49	27	24	milk	milk	NOUN
cvj-49	27	25	proteins	protein	NOUN
cvj-49	27	26	.	.	PUNCT
cvj-49	28	1	if	if	SCONJ
cvj-49	28	2	debris	debris	NOUN
cvj-49	28	3	is	be	AUX
cvj-49	28	4	not	not	PART
cvj-49	28	5	removed	remove	VERB
cvj-49	28	6	or	or	CCONJ
cvj-49	28	7	gated	gate	VERB
cvj-49	28	8	out	out	ADP
cvj-49	28	9	,	,	PUNCT
cvj-49	28	10	it	it	PRON
cvj-49	28	11	can	can	AUX
cvj-49	28	12	lead	lead	VERB
cvj-49	28	13	to	to	ADP
cvj-49	28	14	false	false	ADJ
cvj-49	28	15	results	result	NOUN
cvj-49	28	16	as	as	SCONJ
cvj-49	28	17	foreign	foreign	ADJ
cvj-49	28	18	particles	particle	NOUN
cvj-49	28	19	may	may	AUX
cvj-49	28	20	overlap	overlap	VERB
cvj-49	28	21	unstained	unstained	ADJ
cvj-49	28	22	populations	population	NOUN
cvj-49	28	23	of	of	ADP
cvj-49	28	24	spermatozoa	spermatozoa	NOUN
cvj-49	28	25	[	[	X
cvj-49	28	26	4	4	NUM
cvj-49	28	27	]	]	PUNCT
cvj-49	28	28	.	.	PUNCT
cvj-49	29	1	on	on	ADP
cvj-49	29	2	the	the	DET
cvj-49	29	3	other	other	ADJ
cvj-49	29	4	hand	hand	NOUN
cvj-49	29	5	,	,	PUNCT
cvj-49	29	6	there	there	PRON
cvj-49	29	7	are	be	VERB
cvj-49	29	8	dyes	dye	NOUN
cvj-49	29	9	such	such	ADJ
cvj-49	29	10	as	as	ADP
cvj-49	29	11	tetraethylbenzimidazolylcarbocyanine	tetraethylbenzimidazolylcarbocyanine	NOUN
cvj-49	29	12	iodide	iodide	NOUN
cvj-49	29	13	(	(	PUNCT
cvj-49	29	14	jc-1	jc-1	NOUN
cvj-49	29	15	)	)	PUNCT
cvj-49	29	16	which	which	PRON
cvj-49	29	17	are	be	AUX
cvj-49	29	18	lipophilic	lipophilic	ADJ
cvj-49	29	19	and	and	CCONJ
cvj-49	29	20	therefore	therefore	ADV
cvj-49	29	21	can	can	AUX
cvj-49	29	22	bind	bind	VERB
cvj-49	29	23	to	to	ADP
cvj-49	29	24	debris	debris	NOUN
cvj-49	29	25	and	and	CCONJ
cvj-49	29	26	yield	yield	VERB
cvj-49	29	27	misleading	mislead	VERB
cvj-49	29	28	results	result	NOUN
cvj-49	29	29	.	.	PUNCT
cvj-49	30	1	gating	gate	VERB
cvj-49	30	2	out	out	ADP
cvj-49	30	3	debris	debris	NOUN
cvj-49	30	4	can	can	AUX
cvj-49	30	5	be	be	AUX
cvj-49	30	6	achieved	achieve	VERB
cvj-49	30	7	on	on	ADP
cvj-49	30	8	the	the	DET
cvj-49	30	9	fsc	fsc	PROPN
cvj-49	30	10	vs	vs	PROPN
cvj-49	30	11	ssc	ssc	NOUN
cvj-49	30	12	dot	dot	NOUN
cvj-49	30	13	plot	plot	NOUN
cvj-49	30	14	,	,	PUNCT
cvj-49	30	15	where	where	SCONJ
cvj-49	30	16	it	it	PRON
cvj-49	30	17	usually	usually	ADV
cvj-49	30	18	appears	appear	VERB
cvj-49	30	19	to	to	PART
cvj-49	30	20	have	have	VERB
cvj-49	30	21	significantly	significantly	ADV
cvj-49	30	22	lower	low	ADJ
cvj-49	30	23	fsc	fsc	PROPN
cvj-49	30	24	as	as	ADP
cvj-49	30	25	spermatozoa	spermatozoa	PROPN
cvj-49	30	26	.	.	PUNCT
cvj-49	31	1	mathematical	mathematical	ADJ
cvj-49	31	2	corrections	correction	NOUN
cvj-49	31	3	are	be	AUX
cvj-49	31	4	also	also	ADV
cvj-49	31	5	possible	possible	ADJ
cvj-49	31	6	but	but	CCONJ
cvj-49	31	7	are	be	AUX
cvj-49	31	8	time	time	NOUN
cvj-49	31	9	consuming	consume	VERB
cvj-49	31	10	and	and	CCONJ
cvj-49	31	11	difficult	difficult	ADJ
cvj-49	31	12	to	to	PART
cvj-49	31	13	perform	perform	VERB
cvj-49	31	14	.	.	PUNCT
cvj-49	32	1	another	another	DET
cvj-49	32	2	option	option	NOUN
cvj-49	32	3	is	be	AUX
cvj-49	32	4	to	to	PART
cvj-49	32	5	use	use	VERB
cvj-49	32	6	an	an	DET
cvj-49	32	7	intravital	intravital	ADJ
cvj-49	32	8	dye	dye	NOUN
cvj-49	32	9	,	,	PUNCT
cvj-49	32	10	such	such	ADJ
cvj-49	32	11	as	as	ADP
cvj-49	32	12	hoechst	hoechst	NOUN
cvj-49	32	13	33342	33342	NUM
cvj-49	32	14	which	which	PRON
cvj-49	32	15	only	only	ADV
cvj-49	32	16	stains	stain	VERB
cvj-49	32	17	live	live	ADJ
cvj-49	32	18	cells	cell	NOUN
cvj-49	32	19	,	,	PUNCT
cvj-49	32	20	and	and	CCONJ
cvj-49	32	21	therefore	therefore	ADV
cvj-49	32	22	unstained	unstaine	VERB
cvj-49	32	23	events	event	NOUN
cvj-49	32	24	can	can	AUX
cvj-49	32	25	be	be	AUX
cvj-49	32	26	gated	gate	VERB
cvj-49	32	27	out	out	ADP
cvj-49	32	28	as	as	ADP
cvj-49	32	29	debris	debris	NOUN
cvj-49	32	30	.	.	PUNCT
cvj-49	33	1	the	the	DET
cvj-49	33	2	advantage	advantage	NOUN
cvj-49	33	3	of	of	ADP
cvj-49	33	4	using	use	VERB
cvj-49	33	5	this	this	DET
cvj-49	33	6	dye	dye	NOUN
cvj-49	33	7	also	also	ADV
cvj-49	33	8	resides	reside	VERB
cvj-49	33	9	in	in	ADP
cvj-49	33	10	the	the	DET
cvj-49	33	11	fact	fact	NOUN
cvj-49	33	12	that	that	SCONJ
cvj-49	33	13	its	its	PRON
cvj-49	33	14	fluorescent	fluorescent	ADJ
cvj-49	33	15	signal	signal	NOUN
cvj-49	33	16	(	(	PUNCT
cvj-49	33	17	uv	uv	NOUN
cvj-49	33	18	range	range	NOUN
cvj-49	33	19	)	)	PUNCT
cvj-49	33	20	does	do	AUX
cvj-49	33	21	not	not	PART
cvj-49	33	22	overlap	overlap	VERB
cvj-49	33	23	with	with	ADP
cvj-49	33	24	any	any	PRON
cvj-49	33	25	of	of	ADP
cvj-49	33	26	the	the	DET
cvj-49	33	27	fluorochromes	fluorochrome	NOUN
cvj-49	33	28	frequently	frequently	ADV
cvj-49	33	29	used	use	VERB
cvj-49	33	30	for	for	ADP
cvj-49	33	31	semen	semen	NOUN
cvj-49	33	32	analysis	analysis	NOUN
cvj-49	33	33	[	[	X
cvj-49	33	34	5	5	NUM
cvj-49	33	35	]	]	PUNCT
cvj-49	33	36	.	.	PUNCT
cvj-49	34	1	nevertheless	nevertheless	ADV
cvj-49	34	2	,	,	PUNCT
cvj-49	34	3	the	the	DET
cvj-49	34	4	drawback	drawback	NOUN
cvj-49	34	5	is	be	AUX
cvj-49	34	6	that	that	SCONJ
cvj-49	34	7	the	the	DET
cvj-49	34	8	cytometer	cytometer	NOUN
cvj-49	34	9	must	must	AUX
cvj-49	34	10	have	have	VERB
cvj-49	34	11	a	a	DET
cvj-49	34	12	violet	violet	ADJ
cvj-49	34	13	laser	laser	NOUN
cvj-49	34	14	or	or	CCONJ
cvj-49	34	15	uv	uv	NOUN
cvj-49	34	16	diode	diode	NOUN
cvj-49	34	17	.	.	PUNCT
cvj-49	35	1	3	3	X
cvj-49	35	2	.	.	X
cvj-49	35	3	instrument	instrument	NOUN
cvj-49	35	4	setup	setup	NOUN
cvj-49	35	5	in	in	ADP
cvj-49	35	6	order	order	NOUN
cvj-49	35	7	to	to	PART
cvj-49	35	8	obtain	obtain	VERB
cvj-49	35	9	accurate	accurate	ADJ
cvj-49	35	10	results	result	NOUN
cvj-49	35	11	,	,	PUNCT
cvj-49	35	12	the	the	DET
cvj-49	35	13	flow	flow	NOUN
cvj-49	35	14	cytometer	cytometer	NOUN
cvj-49	35	15	must	must	AUX
cvj-49	35	16	be	be	AUX
cvj-49	35	17	checked	check	VERB
cvj-49	35	18	and	and	CCONJ
cvj-49	35	19	properly	properly	ADV
cvj-49	35	20	calibrated	calibrate	VERB
cvj-49	35	21	on	on	ADP
cvj-49	35	22	a	a	DET
cvj-49	35	23	daily	daily	ADJ
cvj-49	35	24	basis	basis	NOUN
cvj-49	35	25	.	.	PUNCT
cvj-49	36	1	calibration	calibration	NOUN
cvj-49	36	2	microspheres	microsphere	NOUN
cvj-49	36	3	are	be	AUX
cvj-49	36	4	usually	usually	ADV
cvj-49	36	5	made	make	VERB
cvj-49	36	6	of	of	ADP
cvj-49	36	7	polystyrene	polystyrene	NOUN
cvj-49	36	8	latex	latex	NOUN
cvj-49	36	9	and	and	CCONJ
cvj-49	36	10	are	be	AUX
cvj-49	36	11	labelled	label	VERB
cvj-49	36	12	with	with	ADP
cvj-49	36	13	fluorescent	fluorescent	ADJ
cvj-49	36	14	dyes	dye	NOUN
cvj-49	36	15	.	.	PUNCT
cvj-49	37	1	each	each	DET
cvj-49	37	2	laboratory	laboratory	NOUN
cvj-49	37	3	must	must	AUX
cvj-49	37	4	establish	establish	VERB
cvj-49	37	5	a	a	DET
cvj-49	37	6	daily	daily	ADJ
cvj-49	37	7	clean	clean	ADJ
cvj-49	37	8	-	-	PUNCT
cvj-49	37	9	up	up	NOUN
cvj-49	37	10	and	and	CCONJ
cvj-49	37	11	calibration	calibration	NOUN
cvj-49	37	12	protocol	protocol	NOUN
cvj-49	37	13	,	,	PUNCT
cvj-49	37	14	according	accord	VERB
cvj-49	37	15	to	to	ADP
cvj-49	37	16	the	the	DET
cvj-49	37	17	type	type	NOUN
cvj-49	37	18	of	of	ADP
cvj-49	37	19	equipment	equipment	NOUN
cvj-49	37	20	that	that	PRON
cvj-49	37	21	is	be	AUX
cvj-49	37	22	present	present	ADJ
cvj-49	37	23	and	and	CCONJ
cvj-49	37	24	the	the	DET
cvj-49	37	25	instructions	instruction	NOUN
cvj-49	37	26	of	of	ADP
cvj-49	37	27	the	the	DET
cvj-49	37	28	producer	producer	NOUN
cvj-49	37	29	.	.	PUNCT
cvj-49	38	1	standardization	standardization	NOUN
cvj-49	38	2	of	of	ADP
cvj-49	38	3	the	the	DET
cvj-49	38	4	procedure	procedure	NOUN
cvj-49	38	5	is	be	AUX
cvj-49	38	6	very	very	ADV
cvj-49	38	7	important	important	ADJ
cvj-49	38	8	,	,	PUNCT
cvj-49	38	9	as	as	SCONJ
cvj-49	38	10	it	it	PRON
cvj-49	38	11	provides	provide	VERB
cvj-49	38	12	reproducibility	reproducibility	NOUN
cvj-49	38	13	,	,	PUNCT
cvj-49	38	14	accuracy	accuracy	NOUN
cvj-49	38	15	and	and	CCONJ
cvj-49	38	16	reliability	reliability	NOUN
cvj-49	38	17	of	of	ADP
cvj-49	38	18	results	result	NOUN
cvj-49	38	19	.	.	PUNCT
cvj-49	39	1	next	next	ADV
cvj-49	39	2	,	,	PUNCT
cvj-49	39	3	the	the	DET
cvj-49	39	4	machine	machine	NOUN
cvj-49	39	5	can	can	AUX
cvj-49	39	6	be	be	AUX
cvj-49	39	7	prepared	prepare	VERB
cvj-49	39	8	for	for	ADP
cvj-49	39	9	analysis	analysis	NOUN
cvj-49	39	10	,	,	PUNCT
cvj-49	39	11	by	by	ADP
cvj-49	39	12	choosing	choose	VERB
cvj-49	39	13	the	the	DET
cvj-49	39	14	optimum	optimum	ADJ
cvj-49	39	15	channels	channel	NOUN
cvj-49	39	16	and	and	CCONJ
cvj-49	39	17	optical	optical	ADJ
cvj-49	39	18	filters	filter	NOUN
cvj-49	39	19	needed	need	VERB
cvj-49	39	20	,	,	PUNCT
cvj-49	39	21	according	accord	VERB
cvj-49	39	22	to	to	ADP
cvj-49	39	23	the	the	DET
cvj-49	39	24	type	type	NOUN
cvj-49	39	25	of	of	ADP
cvj-49	39	26	experiment	experiment	NOUN
cvj-49	39	27	that	that	PRON
cvj-49	39	28	is	be	AUX
cvj-49	39	29	required	require	VERB
cvj-49	39	30	.	.	PUNCT
cvj-49	40	1	usually	usually	ADV
cvj-49	40	2	,	,	PUNCT
cvj-49	40	3	the	the	DET
cvj-49	40	4	blue	blue	ADJ
cvj-49	40	5	488	488	NUM
cvj-49	40	6	nm	nm	ADJ
cvj-49	40	7	argon	argon	NOUN
cvj-49	40	8	ion	ion	NOUN
cvj-49	40	9	laser	laser	NOUN
cvj-49	40	10	at	at	ADP
cvj-49	40	11	488	488	NUM
cvj-49	40	12	nm	nm	NOUN
cvj-49	40	13	is	be	AUX
cvj-49	40	14	the	the	DET
cvj-49	40	15	only	only	ADJ
cvj-49	40	16	one	one	NOUN
cvj-49	40	17	that	that	PRON
cvj-49	40	18	is	be	AUX
cvj-49	40	19	needed	need	VERB
cvj-49	40	20	,	,	PUNCT
cvj-49	40	21	since	since	SCONJ
cvj-49	40	22	most	most	ADJ
cvj-49	40	23	of	of	ADP
cvj-49	40	24	the	the	DET
cvj-49	40	25	dyes	dye	NOUN
cvj-49	40	26	used	use	VERB
cvj-49	40	27	for	for	ADP
cvj-49	40	28	sperm	sperm	NOUN
cvj-49	40	29	analysis	analysis	NOUN
cvj-49	40	30	emit	emit	NOUN
cvj-49	40	31	green	green	ADJ
cvj-49	40	32	,	,	PUNCT
cvj-49	40	33	orange	orange	ADJ
cvj-49	40	34	or	or	CCONJ
cvj-49	40	35	red	red	ADJ
cvj-49	40	36	fluorescence	fluorescence	NOUN
cvj-49	40	37	.	.	PUNCT
cvj-49	41	1	green	green	ADJ
cvj-49	41	2	fluorescence	fluorescence	NOUN
cvj-49	41	3	is	be	AUX
cvj-49	41	4	read	read	VERB
cvj-49	41	5	in	in	ADP
cvj-49	41	6	fl1	fl1	ADV
cvj-49	41	7	,	,	PUNCT
cvj-49	41	8	orange	orange	ADJ
cvj-49	41	9	in	in	ADP
cvj-49	41	10	fl2	fl2	PROPN
cvj-49	41	11	and	and	CCONJ
cvj-49	41	12	red	red	ADJ
cvj-49	41	13	in	in	ADP
cvj-49	41	14	fl3	fl3	NOUN
cvj-49	41	15	.	.	PUNCT
cvj-49	42	1	regarding	regard	VERB
cvj-49	42	2	filters	filter	NOUN
cvj-49	42	3	,	,	PUNCT
cvj-49	42	4	the	the	DET
cvj-49	42	5	530/28	530/28	NUM
cvj-49	42	6	bp	bp	PROPN
cvj-49	42	7	should	should	AUX
cvj-49	42	8	be	be	AUX
cvj-49	42	9	used	use	VERB
cvj-49	42	10	for	for	ADP
cvj-49	42	11	fl1	fl1	ADV
cvj-49	42	12	,	,	PUNCT
cvj-49	42	13	the	the	DET
cvj-49	42	14	585/42	585/42	NUM
cvj-49	42	15	bp	bp	NOUN
cvj-49	42	16	for	for	ADP
cvj-49	42	17	fl2	fl2	PROPN
cvj-49	42	18	and	and	CCONJ
cvj-49	42	19	the	the	DET
cvj-49	42	20	650	650	NUM
cvj-49	42	21	lp	lp	NOUN
cvj-49	42	22	for	for	ADP
cvj-49	42	23	fl3	fl3	NOUN
cvj-49	42	24	.	.	PUNCT
cvj-49	43	1	all	all	DET
cvj-49	43	2	dot	dot	NOUN
cvj-49	43	3	plots	plot	NOUN
cvj-49	43	4	or	or	CCONJ
cvj-49	43	5	histograms	histogram	NOUN
cvj-49	43	6	should	should	AUX
cvj-49	43	7	be	be	AUX
cvj-49	43	8	set	set	VERB
cvj-49	43	9	to	to	ADP
cvj-49	43	10	the	the	DET
cvj-49	43	11	logarithmic	logarithmic	ADJ
cvj-49	43	12	scale	scale	NOUN
cvj-49	43	13	and	and	CCONJ
cvj-49	43	14	signal	signal	ADJ
cvj-49	43	15	height	height	NOUN
cvj-49	43	16	should	should	AUX
cvj-49	43	17	be	be	AUX
cvj-49	43	18	acquired	acquire	VERB
cvj-49	43	19	for	for	ADP
cvj-49	43	20	all	all	DET
cvj-49	43	21	parameters	parameter	NOUN
cvj-49	43	22	.	.	PUNCT
cvj-49	44	1	compensation	compensation	NOUN
cvj-49	44	2	should	should	AUX
cvj-49	44	3	always	always	ADV
cvj-49	44	4	be	be	AUX
cvj-49	44	5	performed	perform	VERB
cvj-49	44	6	,	,	PUNCT
cvj-49	44	7	whenever	whenever	SCONJ
cvj-49	44	8	multiple	multiple	ADJ
cvj-49	44	9	dyes	dye	NOUN
cvj-49	44	10	are	be	AUX
cvj-49	44	11	used	use	VERB
cvj-49	44	12	to	to	PART
cvj-49	44	13	stain	stain	VERB
cvj-49	44	14	the	the	DET
cvj-49	44	15	same	same	ADJ
cvj-49	44	16	semen	semen	NOUN
cvj-49	44	17	sample	sample	NOUN
cvj-49	44	18	,	,	PUNCT
cvj-49	44	19	in	in	ADP
cvj-49	44	20	order	order	NOUN
cvj-49	44	21	to	to	PART
cvj-49	44	22	avoid	avoid	VERB
cvj-49	44	23	fluorescence	fluorescence	NOUN
cvj-49	44	24	spill	spill	NOUN
cvj-49	44	25	over	over	ADP
cvj-49	44	26	and	and	CCONJ
cvj-49	44	27	false	false	ADJ
cvj-49	44	28	results	result	NOUN
cvj-49	44	29	.	.	PUNCT
cvj-49	45	1	this	this	PRON
cvj-49	45	2	is	be	AUX
cvj-49	45	3	usually	usually	ADV
cvj-49	45	4	achieved	achieve	VERB
cvj-49	45	5	using	use	VERB
cvj-49	45	6	compensation	compensation	NOUN
cvj-49	45	7	beads	bead	NOUN
cvj-49	45	8	,	,	PUNCT
cvj-49	45	9	that	that	PRON
cvj-49	45	10	are	be	AUX
cvj-49	45	11	stained	stain	VERB
cvj-49	45	12	with	with	ADP
cvj-49	45	13	the	the	DET
cvj-49	45	14	same	same	ADJ
cvj-49	45	15	fluorochromes	fluorochrome	NOUN
cvj-49	45	16	as	as	ADP
cvj-49	45	17	those	those	PRON
cvj-49	45	18	used	use	VERB
cvj-49	45	19	in	in	ADP
cvj-49	45	20	the	the	DET
cvj-49	45	21	experiment	experiment	NOUN
cvj-49	45	22	.	.	PUNCT
cvj-49	46	1	experienced	experience	VERB
cvj-49	46	2	users	user	NOUN
cvj-49	46	3	may	may	AUX
cvj-49	46	4	also	also	ADV
cvj-49	46	5	choose	choose	VERB
cvj-49	46	6	to	to	PART
cvj-49	46	7	perform	perform	VERB
cvj-49	46	8	manual	manual	ADJ
cvj-49	46	9	compensation	compensation	NOUN
cvj-49	46	10	,	,	PUNCT
cvj-49	46	11	after	after	SCONJ
cvj-49	46	12	the	the	DET
cvj-49	46	13	samples	sample	NOUN
cvj-49	46	14	are	be	AUX
cvj-49	46	15	acquired	acquire	VERB
cvj-49	46	16	.	.	PUNCT
cvj-49	47	1	actual	actual	ADJ
cvj-49	47	2	analysis	analysis	NOUN
cvj-49	47	3	begins	begin	VERB
cvj-49	47	4	by	by	ADP
cvj-49	47	5	plotting	plot	VERB
cvj-49	47	6	forward	forward	ADV
cvj-49	47	7	scatter	scatter	NOUN
cvj-49	47	8	height	height	NOUN
cvj-49	47	9	(	(	PUNCT
cvj-49	47	10	fsc	fsc	PROPN
cvj-49	47	11	-	-	PUNCT
cvj-49	47	12	h	h	NOUN
cvj-49	47	13	)	)	PUNCT
cvj-49	47	14	vs	vs	ADP
cvj-49	47	15	side	side	NOUN
cvj-49	47	16	scatter	scatter	NOUN
cvj-49	47	17	height	height	NOUN
cvj-49	47	18	(	(	PUNCT
cvj-49	47	19	ssc	ssc	NOUN
cvj-49	47	20	-	-	PUNCT
cvj-49	47	21	h	h	NOUN
cvj-49	47	22	)	)	PUNCT
cvj-49	47	23	in	in	ADP
cvj-49	47	24	order	order	NOUN
cvj-49	47	25	to	to	PART
cvj-49	47	26	define	define	VERB
cvj-49	47	27	and	and	CCONJ
cvj-49	47	28	gate	gate	VERB
cvj-49	47	29	the	the	DET
cvj-49	47	30	sperm	sperm	NOUN
cvj-49	47	31	population	population	NOUN
cvj-49	47	32	.	.	PUNCT
cvj-49	48	1	this	this	PRON
cvj-49	48	2	will	will	AUX
cvj-49	48	3	also	also	ADV
cvj-49	48	4	allow	allow	VERB
cvj-49	48	5	to	to	PART
cvj-49	48	6	remove	remove	VERB
cvj-49	48	7	from	from	ADP
cvj-49	48	8	the	the	DET
cvj-49	48	9	gate	gate	NOUN
cvj-49	48	10	any	any	DET
cvj-49	48	11	debris	debris	NOUN
cvj-49	48	12	(	(	PUNCT
cvj-49	48	13	mostly	mostly	ADV
cvj-49	48	14	originating	originate	VERB
cvj-49	48	15	from	from	ADP
cvj-49	48	16	the	the	DET
cvj-49	48	17	extenders	extender	NOUN
cvj-49	48	18	)	)	PUNCT
cvj-49	48	19	and	and	CCONJ
cvj-49	48	20	also	also	ADV
cvj-49	48	21	to	to	PART
cvj-49	48	22	eliminate	eliminate	VERB
cvj-49	48	23	any	any	DET
cvj-49	48	24	electronic	electronic	ADJ
cvj-49	48	25	noise	noise	NOUN
cvj-49	48	26	.	.	PUNCT
cvj-49	49	1	a	a	DET
cvj-49	49	2	total	total	ADJ
cvj-49	49	3	number	number	NOUN
cvj-49	49	4	of	of	ADP
cvj-49	49	5	at	at	ADV
cvj-49	49	6	least	least	ADV
cvj-49	49	7	10,000	10,000	NUM
cvj-49	49	8	events	event	NOUN
cvj-49	49	9	should	should	AUX
cvj-49	49	10	be	be	AUX
cvj-49	49	11	acquired	acquire	VERB
cvj-49	49	12	,	,	PUNCT
cvj-49	49	13	and	and	CCONJ
cvj-49	49	14	,	,	PUNCT
cvj-49	49	15	since	since	SCONJ
cvj-49	49	16	the	the	DET
cvj-49	49	17	cells	cell	NOUN
cvj-49	49	18	(	(	PUNCT
cvj-49	49	19	spermatozoa	spermatozoa	NOUN
cvj-49	49	20	)	)	PUNCT
cvj-49	49	21	are	be	AUX
cvj-49	49	22	small	small	ADJ
cvj-49	49	23	,	,	PUNCT
cvj-49	49	24	the	the	DET
cvj-49	49	25	sample	sample	NOUN
cvj-49	49	26	should	should	AUX
cvj-49	49	27	be	be	AUX
cvj-49	49	28	run	run	VERB
cvj-49	49	29	at	at	ADP
cvj-49	49	30	low	low	ADJ
cvj-49	49	31	speed	speed	NOUN
cvj-49	49	32	for	for	ADP
cvj-49	49	33	better	well	ADJ
cvj-49	49	34	accuracy	accuracy	NOUN
cvj-49	49	35	.	.	PUNCT
cvj-49	50	1	4	4	X
cvj-49	50	2	.	.	X
cvj-49	50	3	sperm	sperm	NOUN
cvj-49	50	4	viability	viability	NOUN
cvj-49	50	5	assessment	assessment	NOUN
cvj-49	50	6	dna	dna	PROPN
cvj-49	50	7	intercalating	intercalate	VERB
cvj-49	50	8	agents	agent	NOUN
cvj-49	50	9	are	be	AUX
cvj-49	50	10	fluorescent	fluorescent	ADJ
cvj-49	50	11	molecules	molecule	NOUN
cvj-49	50	12	,	,	PUNCT
cvj-49	50	13	capable	capable	ADJ
cvj-49	50	14	of	of	ADP
cvj-49	50	15	passing	pass	VERB
cvj-49	50	16	through	through	ADP
cvj-49	50	17	cellular	cellular	ADJ
cvj-49	50	18	membranes	membrane	NOUN
cvj-49	50	19	of	of	ADP
cvj-49	50	20	cells	cell	NOUN
cvj-49	50	21	and	and	CCONJ
cvj-49	50	22	therefore	therefore	ADV
cvj-49	50	23	stain	stain	VERB
cvj-49	50	24	the	the	DET
cvj-49	50	25	nuclei	nucleus	NOUN
cvj-49	50	26	.	.	PUNCT
cvj-49	51	1	some	some	PRON
cvj-49	51	2	of	of	ADP
cvj-49	51	3	the	the	DET
cvj-49	51	4	molecules	molecule	NOUN
cvj-49	51	5	are	be	AUX
cvj-49	51	6	able	able	ADJ
cvj-49	51	7	to	to	PART
cvj-49	51	8	pass	pass	VERB
cvj-49	51	9	through	through	ADP
cvj-49	51	10	intact	intact	ADJ
cvj-49	51	11	membranes	membrane	NOUN
cvj-49	51	12	and	and	CCONJ
cvj-49	51	13	therefore	therefore	ADV
cvj-49	51	14	also	also	ADV
cvj-49	51	15	stain	stain	VERB
cvj-49	51	16	the	the	DET
cvj-49	51	17	nuclei	nucleus	NOUN
cvj-49	51	18	of	of	ADP
cvj-49	51	19	live	live	ADJ
cvj-49	51	20	cells	cell	NOUN
cvj-49	51	21	.	.	PUNCT
cvj-49	52	1	a	a	DET
cvj-49	52	2	frequently	frequently	ADV
cvj-49	52	3	utilized	utilize	VERB
cvj-49	52	4	green	green	ADJ
cvj-49	52	5	fluorescent	fluorescent	NOUN
cvj-49	52	6	dye	dye	NOUN
cvj-49	52	7	,	,	PUNCT
cvj-49	52	8	that	that	PRON
cvj-49	52	9	stains	stain	VERB
cvj-49	52	10	all	all	DET
cvj-49	52	11	nuclei	nucleus	NOUN
cvj-49	52	12	of	of	ADP
cvj-49	52	13	spermatozoa	spermatozoon	NOUN
cvj-49	52	14	(	(	PUNCT
cvj-49	52	15	damaged	damage	VERB
cvj-49	52	16	or	or	CCONJ
cvj-49	52	17	intact	intact	ADJ
cvj-49	52	18	)	)	PUNCT
cvj-49	52	19	,	,	PUNCT
cvj-49	52	20	is	be	AUX
cvj-49	52	21	sybr-14	sybr-14	ADJ
cvj-49	52	22	(	(	PUNCT
cvj-49	52	23	maximum	maximum	ADJ
cvj-49	52	24	emission	emission	NOUN
cvj-49	52	25	at	at	ADP
cvj-49	52	26	516	516	NUM
cvj-49	52	27	nm	nm	NOUN
cvj-49	52	28	)	)	PUNCT
cvj-49	52	29	which	which	PRON
cvj-49	52	30	is	be	AUX
cvj-49	52	31	used	use	VERB
cvj-49	52	32	in	in	ADP
cvj-49	52	33	combination	combination	NOUN
cvj-49	52	34	with	with	ADP
cvj-49	52	35	a	a	DET
cvj-49	52	36	red	red	ADJ
cvj-49	52	37	dye	dye	NOUN
cvj-49	52	38	propidium	propidium	NOUN
cvj-49	52	39	iodide	iodide	NOUN
cvj-49	52	40	(	(	PUNCT
cvj-49	52	41	pi	pi	NOUN
cvj-49	52	42	,	,	PUNCT
cvj-49	52	43	maximum	maximum	ADJ
cvj-49	52	44	emission	emission	NOUN
cvj-49	52	45	at	at	ADP
cvj-49	52	46	617	617	NUM
cvj-49	52	47	nm	nm	NOUN
cvj-49	52	48	)	)	PUNCT
cvj-49	52	49	.	.	PUNCT
cvj-49	53	1	the	the	DET
cvj-49	53	2	latter	latter	ADJ
cvj-49	53	3	is	be	AUX
cvj-49	53	4	also	also	ADV
cvj-49	53	5	an	an	DET
cvj-49	53	6	intercalating	intercalate	VERB
cvj-49	53	7	agent	agent	NOUN
cvj-49	53	8	which	which	PRON
cvj-49	53	9	can	can	AUX
cvj-49	53	10	only	only	ADV
cvj-49	53	11	penetrate	penetrate	VERB
cvj-49	53	12	the	the	DET
cvj-49	53	13	damaged	damage	VERB
cvj-49	53	14	plasma	plasma	NOUN
cvj-49	53	15	membrane	membrane	NOUN
cvj-49	53	16	and	and	CCONJ
cvj-49	53	17	therefore	therefore	ADV
cvj-49	53	18	stains	stain	VERB
cvj-49	53	19	the	the	DET
cvj-49	53	20	nucleus	nucleus	NOUN
cvj-49	53	21	of	of	ADP
cvj-49	53	22	only	only	ADJ
cvj-49	53	23	dead	dead	ADJ
cvj-49	53	24	spermatozoa	spermatozoon	NOUN
cvj-49	53	25	.	.	PUNCT
cvj-49	54	1	pi	pi	PROPN
cvj-49	54	2	signal	signal	PROPN
cvj-49	54	3	quenches	quench	VERB
cvj-49	54	4	sybr-14	sybr-14	ADJ
cvj-49	54	5	fluorescence	fluorescence	NOUN
cvj-49	54	6	,	,	PUNCT
cvj-49	54	7	thus	thus	ADV
cvj-49	54	8	live	live	ADJ
cvj-49	54	9	spermatozoa	spermatozoon	NOUN
cvj-49	54	10	are	be	AUX
cvj-49	54	11	stained	stain	VERB
cvj-49	54	12	in	in	ADP
cvj-49	54	13	green	green	ADJ
cvj-49	54	14	while	while	SCONJ
cvj-49	54	15	dead	dead	ADJ
cvj-49	54	16	spermatozoa	spermatozoon	NOUN
cvj-49	54	17	are	be	AUX
cvj-49	54	18	red	red	ADJ
cvj-49	54	19	[	[	X
cvj-49	54	20	6	6	NUM
cvj-49	54	21	]	]	PUNCT
cvj-49	54	22	.	.	PUNCT
cvj-49	55	1	nowadays	nowadays	ADV
cvj-49	55	2	,	,	PUNCT
cvj-49	55	3	there	there	PRON
cvj-49	55	4	are	be	VERB
cvj-49	55	5	several	several	ADJ
cvj-49	55	6	live	live	ADJ
cvj-49	55	7	/	/	SYM
cvj-49	55	8	dead	dead	ADJ
cvj-49	55	9	kits	kit	NOUN
cvj-49	55	10	available	available	ADJ
cvj-49	55	11	on	on	ADP
cvj-49	55	12	the	the	DET
cvj-49	55	13	marked	mark	VERB
cvj-49	55	14	,	,	PUNCT
cvj-49	55	15	which	which	PRON
cvj-49	55	16	allow	allow	VERB
cvj-49	55	17	differentiation	differentiation	NOUN
cvj-49	55	18	between	between	ADP
cvj-49	55	19	live	live	ADJ
cvj-49	55	20	and	and	CCONJ
cvj-49	55	21	dead	dead	ADJ
cvj-49	55	22	spermatozoa	spermatozoon	NOUN
cvj-49	55	23	.	.	PUNCT
cvj-49	56	1	those	those	DET
cvj-49	56	2	kits	kit	NOUN
cvj-49	56	3	also	also	ADV
cvj-49	56	4	permit	permit	VERB
cvj-49	56	5	an	an	DET
cvj-49	56	6	easy	easy	ADJ
cvj-49	56	7	cluj	cluj	NOUN
cvj-49	56	8	vet	vet	NOUN
cvj-49	56	9	j	j	PROPN
cvj-49	56	10	2023	2023	NUM
cvj-49	56	11	,	,	PUNCT
cvj-49	56	12	vol	vol	NOUN
cvj-49	56	13	28	28	NUM
cvj-49	56	14	,	,	PUNCT
cvj-49	56	15	issue	issue	VERB
cvj-49	56	16	46	46	NUM
cvj-49	56	17	discrimination	discrimination	NOUN
cvj-49	56	18	between	between	ADP
cvj-49	56	19	spermatozoa	spermatozoon	NOUN
cvj-49	56	20	and	and	CCONJ
cvj-49	56	21	debris	debris	NOUN
cvj-49	56	22	,	,	PUNCT
cvj-49	56	23	and	and	CCONJ
cvj-49	56	24	therefore	therefore	ADV
cvj-49	56	25	eliminate	eliminate	VERB
cvj-49	56	26	the	the	DET
cvj-49	56	27	need	need	NOUN
cvj-49	56	28	of	of	ADP
cvj-49	56	29	performing	perform	VERB
cvj-49	56	30	additional	additional	ADJ
cvj-49	56	31	staining	staining	ADJ
cvj-49	56	32	steps	step	NOUN
cvj-49	56	33	.	.	PUNCT
cvj-49	57	1	these	these	DET
cvj-49	57	2	kits	kit	NOUN
cvj-49	57	3	usually	usually	ADV
cvj-49	57	4	contain	contain	VERB
cvj-49	57	5	a	a	DET
cvj-49	57	6	1	1	NUM
cvj-49	57	7	mm	mm	NOUN
cvj-49	57	8	solution	solution	NOUN
cvj-49	57	9	of	of	ADP
cvj-49	57	10	sybr-14	sybr-14	PROPN
cvj-49	57	11	in	in	ADP
cvj-49	57	12	dmso	dmso	NOUN
cvj-49	57	13	and	and	CCONJ
cvj-49	57	14	a	a	DET
cvj-49	57	15	2.4	2.4	NUM
cvj-49	57	16	mm	mm	NOUN
cvj-49	57	17	solution	solution	NOUN
cvj-49	57	18	of	of	ADP
cvj-49	57	19	pi	pi	NOUN
cvj-49	57	20	in	in	ADP
cvj-49	57	21	water	water	NOUN
cvj-49	57	22	.	.	PUNCT
cvj-49	58	1	a	a	DET
cvj-49	58	2	20	20	NUM
cvj-49	58	3	µm	µm	ADP
cvj-49	58	4	sybr-14	sybr-14	ADJ
cvj-49	58	5	stock	stock	NOUN
cvj-49	58	6	solution	solution	NOUN
cvj-49	58	7	in	in	ADP
cvj-49	58	8	dmso	dmso	NOUN
cvj-49	58	9	is	be	AUX
cvj-49	58	10	initially	initially	ADV
cvj-49	58	11	prepared	prepare	VERB
cvj-49	58	12	,	,	PUNCT
cvj-49	58	13	which	which	PRON
cvj-49	58	14	can	can	AUX
cvj-49	58	15	be	be	AUX
cvj-49	58	16	stored	store	VERB
cvj-49	58	17	frozen	frozen	ADJ
cvj-49	58	18	.	.	PUNCT
cvj-49	59	1	when	when	SCONJ
cvj-49	59	2	needed	need	VERB
cvj-49	59	3	,	,	PUNCT
cvj-49	59	4	the	the	DET
cvj-49	59	5	working	work	VERB
cvj-49	59	6	solution	solution	NOUN
cvj-49	59	7	is	be	AUX
cvj-49	59	8	made	make	VERB
cvj-49	59	9	by	by	ADP
cvj-49	59	10	adding	add	VERB
cvj-49	59	11	5	5	NUM
cvj-49	59	12	µl	µl	NOUN
cvj-49	59	13	of	of	ADP
cvj-49	59	14	the	the	DET
cvj-49	59	15	20	20	NUM
cvj-49	59	16	µm	µm	ADP
cvj-49	59	17	sybr-14	sybr-14	ADJ
cvj-49	59	18	solution	solution	NOUN
cvj-49	59	19	and	and	CCONJ
cvj-49	59	20	50	50	NUM
cvj-49	59	21	µl	µl	NOUN
cvj-49	59	22	of	of	ADP
cvj-49	59	23	the	the	DET
cvj-49	59	24	2.4	2.4	NUM
cvj-49	59	25	mm	mm	NOUN
cvj-49	59	26	pi	pi	NOUN
cvj-49	59	27	solution	solution	NOUN
cvj-49	59	28	to	to	ADP
cvj-49	59	29	10	10	NUM
cvj-49	59	30	ml	ml	NOUN
cvj-49	59	31	of	of	ADP
cvj-49	59	32	buffer	buffer	NOUN
cvj-49	59	33	,	,	PUNCT
cvj-49	59	34	such	such	ADJ
cvj-49	59	35	as	as	ADP
cvj-49	59	36	hepes	hepe	NOUN
cvj-49	59	37	for	for	ADP
cvj-49	59	38	approximately	approximately	ADV
cvj-49	59	39	20	20	NUM
cvj-49	59	40	samples	sample	NOUN
cvj-49	59	41	.	.	PUNCT
cvj-49	60	1	semen	semen	NOUN
cvj-49	60	2	samples	sample	NOUN
cvj-49	60	3	can	can	AUX
cvj-49	60	4	be	be	AUX
cvj-49	60	5	successfully	successfully	ADV
cvj-49	60	6	stained	stain	VERB
cvj-49	60	7	with	with	ADP
cvj-49	60	8	this	this	DET
cvj-49	60	9	working	work	VERB
cvj-49	60	10	solution	solution	NOUN
cvj-49	60	11	,	,	PUNCT
cvj-49	60	12	although	although	SCONJ
cvj-49	60	13	the	the	DET
cvj-49	60	14	manufacturers	manufacturer	NOUN
cvj-49	60	15	recommend	recommend	VERB
cvj-49	60	16	separate	separate	ADJ
cvj-49	60	17	incubations	incubation	NOUN
cvj-49	60	18	.	.	PUNCT
cvj-49	61	1	spermatozoa	spermatozoon	NOUN
cvj-49	61	2	should	should	AUX
cvj-49	61	3	be	be	AUX
cvj-49	61	4	diluted	dilute	VERB
cvj-49	61	5	to	to	ADP
cvj-49	61	6	a	a	DET
cvj-49	61	7	concentration	concentration	NOUN
cvj-49	61	8	of	of	ADP
cvj-49	61	9	1	1	NUM
cvj-49	61	10	-	-	PUNCT
cvj-49	61	11	2x106	2x106	NUM
cvj-49	61	12	/	/	SYM
cvj-49	61	13	ml	ml	NOUN
cvj-49	61	14	in	in	ADP
cvj-49	61	15	0.5	0.5	NUM
cvj-49	61	16	ml	ml	NOUN
cvj-49	61	17	staining	stain	VERB
cvj-49	61	18	solution	solution	NOUN
cvj-49	61	19	,	,	PUNCT
cvj-49	61	20	in	in	ADP
cvj-49	61	21	cytometry	cytometry	PROPN
cvj-49	61	22	tubes	tube	NOUN
cvj-49	61	23	.	.	PUNCT
cvj-49	62	1	incubation	incubation	NOUN
cvj-49	62	2	should	should	AUX
cvj-49	62	3	be	be	AUX
cvj-49	62	4	made	make	VERB
cvj-49	62	5	in	in	ADP
cvj-49	62	6	the	the	DET
cvj-49	62	7	dark	dark	NOUN
cvj-49	62	8	at	at	ADP
cvj-49	62	9	37	37	NUM
cvj-49	62	10	°	°	ADP
cvj-49	62	11	c	c	NOUN
cvj-49	62	12	and	and	CCONJ
cvj-49	62	13	run	run	VERB
cvj-49	62	14	in	in	ADP
cvj-49	62	15	the	the	DET
cvj-49	62	16	cytometer	cytometer	NOUN
cvj-49	62	17	right	right	ADV
cvj-49	62	18	away	away	ADV
cvj-49	62	19	.	.	PUNCT
cvj-49	63	1	two	two	NUM
cvj-49	63	2	dot	dot	NOUN
cvj-49	63	3	plots	plot	NOUN
cvj-49	63	4	are	be	AUX
cvj-49	63	5	needed	need	VERB
cvj-49	63	6	for	for	ADP
cvj-49	63	7	each	each	DET
cvj-49	63	8	tube	tube	NOUN
cvj-49	63	9	,	,	PUNCT
cvj-49	63	10	one	one	NUM
cvj-49	63	11	for	for	ADP
cvj-49	63	12	spermatozoa	spermatozoa	ADJ
cvj-49	63	13	gating	gate	VERB
cvj-49	63	14	(	(	PUNCT
cvj-49	63	15	fsc	fsc	PROPN
cvj-49	63	16	-	-	PUNCT
cvj-49	63	17	h	h	PROPN
cvj-49	63	18	vs	vs	ADP
cvj-49	63	19	ssc	ssc	NOUN
cvj-49	63	20	-	-	PUNCT
cvj-49	63	21	h	h	NOUN
cvj-49	63	22	)	)	PUNCT
cvj-49	63	23	and	and	CCONJ
cvj-49	63	24	another	another	DET
cvj-49	63	25	one	one	NOUN
cvj-49	63	26	for	for	ADP
cvj-49	63	27	viability	viability	NOUN
cvj-49	63	28	assessment	assessment	NOUN
cvj-49	63	29	(	(	PUNCT
cvj-49	63	30	fl1	fl1	ADV
cvj-49	63	31	-	-	PUNCT
cvj-49	63	32	h	h	NOUN
cvj-49	63	33	vs	vs	ADP
cvj-49	63	34	fl3	fl3	NOUN
cvj-49	63	35	-	-	PUNCT
cvj-49	63	36	h	h	NOUN
cvj-49	63	37	)	)	PUNCT
cvj-49	63	38	.	.	PUNCT
cvj-49	64	1	following	follow	VERB
cvj-49	64	2	the	the	DET
cvj-49	64	3	analysis	analysis	NOUN
cvj-49	64	4	,	,	PUNCT
cvj-49	64	5	3	3	NUM
cvj-49	64	6	distinct	distinct	ADJ
cvj-49	64	7	populations	population	NOUN
cvj-49	64	8	are	be	AUX
cvj-49	64	9	visible	visible	ADJ
cvj-49	64	10	on	on	ADP
cvj-49	64	11	the	the	DET
cvj-49	64	12	dot	dot	NOUN
cvj-49	64	13	plot	plot	NOUN
cvj-49	64	14	:	:	PUNCT
cvj-49	64	15	•	•	NUM
cvj-49	64	16	sybr-14+/pi	sybr-14+/pi	NOUN
cvj-49	64	17	–	–	PUNCT
cvj-49	64	18	(	(	PUNCT
cvj-49	64	19	live	live	ADJ
cvj-49	64	20	spermatozoa	spermatozoon	NOUN
cvj-49	64	21	)	)	PUNCT
cvj-49	64	22	;	;	PUNCT
cvj-49	64	23	•	•	NUM
cvj-49	64	24	sybr-14+/pi+	sybr-14+/pi+	PROPN
cvj-49	64	25	(	(	PUNCT
cvj-49	64	26	moribund	moribund	ADJ
cvj-49	64	27	spermatozoa	spermatozoon	NOUN
cvj-49	64	28	)	)	PUNCT
cvj-49	64	29	;	;	PUNCT
cvj-49	64	30	•	•	X
cvj-49	64	31	sybr-14–/pi+	sybr-14–/pi+	PROPN
cvj-49	64	32	(	(	PUNCT
cvj-49	64	33	dead	dead	ADJ
cvj-49	64	34	spermatozoa	spermatozoa	NOUN
cvj-49	64	35	)	)	PUNCT
cvj-49	64	36	.	.	PUNCT
cvj-49	65	1	the	the	DET
cvj-49	65	2	sybr-14–/pi	sybr-14–/pi	PROPN
cvj-49	65	3	–	–	PUNCT
cvj-49	65	4	events	event	NOUN
cvj-49	65	5	are	be	AUX
cvj-49	65	6	likely	likely	ADJ
cvj-49	65	7	to	to	PART
cvj-49	65	8	represent	represent	VERB
cvj-49	65	9	debris	debris	NOUN
cvj-49	65	10	which	which	PRON
cvj-49	65	11	should	should	AUX
cvj-49	65	12	be	be	AUX
cvj-49	65	13	gated	gate	VERB
cvj-49	65	14	out	out	ADP
cvj-49	65	15	.	.	PUNCT
cvj-49	66	1	alternatively	alternatively	ADV
cvj-49	66	2	,	,	PUNCT
cvj-49	66	3	sperm	sperm	NOUN
cvj-49	66	4	viability	viability	NOUN
cvj-49	66	5	can	can	AUX
cvj-49	66	6	be	be	AUX
cvj-49	66	7	assessed	assess	VERB
cvj-49	66	8	using	use	VERB
cvj-49	66	9	a	a	DET
cvj-49	66	10	combination	combination	NOUN
cvj-49	66	11	of	of	ADP
cvj-49	66	12	3	3	NUM
cvj-49	66	13	fluorescent	fluorescent	ADJ
cvj-49	66	14	dyes	dye	NOUN
cvj-49	66	15	snarf-1	snarf-1	PROPN
cvj-49	66	16	,	,	PUNCT
cvj-49	66	17	yo	yo	NOUN
cvj-49	66	18	-	-	PUNCT
cvj-49	66	19	pro-1	pro-1	NOUN
cvj-49	66	20	and	and	CCONJ
cvj-49	66	21	ethidium	ethidium	NOUN
cvj-49	66	22	homodimer	homodimer	NOUN
cvj-49	66	23	.	.	PUNCT
cvj-49	67	1	thus	thus	ADV
cvj-49	67	2	,	,	PUNCT
cvj-49	67	3	4	4	NUM
cvj-49	67	4	subpopulations	subpopulation	NOUN
cvj-49	67	5	of	of	ADP
cvj-49	67	6	spermatozoa	spermatozoa	NOUN
cvj-49	67	7	can	can	AUX
cvj-49	67	8	be	be	AUX
cvj-49	67	9	detected	detect	VERB
cvj-49	67	10	:	:	PUNCT
cvj-49	67	11	one	one	NUM
cvj-49	67	12	viable	viable	ADJ
cvj-49	67	13	,	,	PUNCT
cvj-49	67	14	with	with	ADP
cvj-49	67	15	stable	stable	ADJ
cvj-49	67	16	membranes	membrane	NOUN
cvj-49	67	17	(	(	PUNCT
cvj-49	67	18	snarf-1	snarf-1	NOUN
cvj-49	67	19	+	+	NOUN
cvj-49	67	20	)	)	PUNCT
cvj-49	67	21	,	,	PUNCT
cvj-49	67	22	and	and	CCONJ
cvj-49	67	23	three	three	NUM
cvj-49	67	24	with	with	ADP
cvj-49	67	25	compromised	compromised	ADJ
cvj-49	67	26	membranes	membrane	NOUN
cvj-49	67	27	:	:	PUNCT
cvj-49	67	28	yo	yo	NOUN
cvj-49	67	29	-	-	PUNCT
cvj-49	67	30	pro-1+/eth-	pro-1+/eth-	NOUN
cvj-49	67	31	,	,	PUNCT
cvj-49	67	32	yo	yo	PROPN
cvj-49	67	33	-	-	PUNCT
cvj-49	67	34	pro1-/eth+	pro1-/eth+	PROPN
cvj-49	67	35	and	and	CCONJ
cvj-49	67	36	yo	yo	PROPN
cvj-49	67	37	-	-	PUNCT
cvj-49	67	38	pro-1+/eth+	pro-1+/eth+	NOUN
cvj-49	67	39	[	[	X
cvj-49	67	40	7,8	7,8	NUM
cvj-49	67	41	]	]	PUNCT
cvj-49	67	42	.	.	PUNCT
cvj-49	68	1	5	5	X
cvj-49	68	2	.	.	X
cvj-49	68	3	evaluation	evaluation	NOUN
cvj-49	68	4	of	of	ADP
cvj-49	68	5	acrosome	acrosome	NOUN
cvj-49	68	6	reaction	reaction	NOUN
cvj-49	68	7	the	the	DET
cvj-49	68	8	acrosome	acrosome	NOUN
cvj-49	68	9	is	be	AUX
cvj-49	68	10	a	a	DET
cvj-49	68	11	structure	structure	NOUN
cvj-49	68	12	that	that	PRON
cvj-49	68	13	covers	cover	VERB
cvj-49	68	14	the	the	DET
cvj-49	68	15	anterior	anterior	ADJ
cvj-49	68	16	part	part	NOUN
cvj-49	68	17	of	of	ADP
cvj-49	68	18	spermatozoa	spermatozoon	NOUN
cvj-49	68	19	in	in	ADP
cvj-49	68	20	mammals	mammal	NOUN
cvj-49	68	21	and	and	CCONJ
cvj-49	68	22	contains	contain	VERB
cvj-49	68	23	enzymes	enzyme	NOUN
cvj-49	68	24	that	that	PRON
cvj-49	68	25	allow	allow	VERB
cvj-49	68	26	penetration	penetration	NOUN
cvj-49	68	27	of	of	ADP
cvj-49	68	28	the	the	DET
cvj-49	68	29	zona	zona	PROPN
cvj-49	68	30	pellucida	pellucida	PROPN
cvj-49	68	31	during	during	ADP
cvj-49	68	32	fertilization	fertilization	NOUN
cvj-49	68	33	.	.	PUNCT
cvj-49	69	1	semen	semen	PROPN
cvj-49	69	2	cryopreservation	cryopreservation	NOUN
cvj-49	69	3	sometimes	sometimes	ADV
cvj-49	69	4	induces	induce	VERB
cvj-49	69	5	a	a	DET
cvj-49	69	6	so	so	ADV
cvj-49	69	7	-	-	PUNCT
cvj-49	69	8	called	call	VERB
cvj-49	69	9	acrosome	acrosome	NOUN
cvj-49	69	10	reaction	reaction	NOUN
cvj-49	69	11	,	,	PUNCT
cvj-49	69	12	which	which	PRON
cvj-49	69	13	means	mean	VERB
cvj-49	69	14	inactivation	inactivation	NOUN
cvj-49	69	15	of	of	ADP
cvj-49	69	16	the	the	DET
cvj-49	69	17	specific	specific	ADJ
cvj-49	69	18	acrosomal	acrosomal	NOUN
cvj-49	69	19	enzymes	enzyme	NOUN
cvj-49	69	20	,	,	PUNCT
cvj-49	69	21	which	which	PRON
cvj-49	69	22	renders	render	VERB
cvj-49	69	23	spermatozoa	spermatozoon	NOUN
cvj-49	69	24	inefficient	inefficient	ADJ
cvj-49	69	25	[	[	X
cvj-49	69	26	9	9	NUM
cvj-49	69	27	]	]	PUNCT
cvj-49	69	28	.	.	PUNCT
cvj-49	70	1	acrosome	acrosome	NOUN
cvj-49	70	2	integrity	integrity	NOUN
cvj-49	70	3	can	can	AUX
cvj-49	70	4	be	be	AUX
cvj-49	70	5	assessed	assess	VERB
cvj-49	70	6	using	use	VERB
cvj-49	70	7	fitc	fitc	NOUN
cvj-49	70	8	or	or	CCONJ
cvj-49	70	9	pe	pe	ADP
cvj-49	70	10	labelled	label	VERB
cvj-49	70	11	plant	plant	NOUN
cvj-49	70	12	lectins	lectin	NOUN
cvj-49	70	13	(	(	PUNCT
cvj-49	70	14	pea	pea	NOUN
cvj-49	70	15	agglutinin	agglutinin	NOUN
cvj-49	70	16	-	-	PUNCT
cvj-49	70	17	psa	psa	NOUN
cvj-49	70	18	or	or	CCONJ
cvj-49	70	19	peanut	peanut	NOUN
cvj-49	70	20	agglutinin	agglutinin	NOUN
cvj-49	70	21	-	-	PUNCT
cvj-49	70	22	pna	pna	NOUN
cvj-49	70	23	)	)	PUNCT
cvj-49	70	24	.	.	PUNCT
cvj-49	71	1	psa	psa	NOUN
cvj-49	71	2	can	can	AUX
cvj-49	71	3	not	not	PART
cvj-49	71	4	breach	breach	VERB
cvj-49	71	5	the	the	DET
cvj-49	71	6	membrane	membrane	NOUN
cvj-49	71	7	of	of	ADP
cvj-49	71	8	an	an	DET
cvj-49	71	9	intact	intact	ADJ
cvj-49	71	10	acrosome	acrosome	NOUN
cvj-49	71	11	and	and	CCONJ
cvj-49	71	12	thus	thus	ADV
cvj-49	71	13	,	,	PUNCT
cvj-49	71	14	if	if	SCONJ
cvj-49	71	15	stained	stain	VERB
cvj-49	71	16	,	,	PUNCT
cvj-49	71	17	spermatozoa	spermatozoon	NOUN
cvj-49	71	18	are	be	AUX
cvj-49	71	19	judged	judge	VERB
cvj-49	71	20	as	as	ADP
cvj-49	71	21	damaged	damage	VERB
cvj-49	71	22	.	.	PUNCT
cvj-49	72	1	in	in	ADP
cvj-49	72	2	practice	practice	NOUN
cvj-49	72	3	,	,	PUNCT
cvj-49	72	4	pna	pna	PROPN
cvj-49	72	5	is	be	AUX
cvj-49	72	6	preferred	prefer	VERB
cvj-49	72	7	to	to	PART
cvj-49	72	8	psa	psa	VERB
cvj-49	72	9	as	as	SCONJ
cvj-49	72	10	the	the	DET
cvj-49	72	11	latter	latter	ADJ
cvj-49	72	12	was	be	AUX
cvj-49	72	13	demonstrated	demonstrate	VERB
cvj-49	72	14	to	to	ADP
cvj-49	72	15	non	non	ADJ
cvj-49	72	16	-	-	ADJ
cvj-49	72	17	specifically	specifically	ADV
cvj-49	72	18	bind	bind	VERB
cvj-49	72	19	to	to	ADP
cvj-49	72	20	the	the	DET
cvj-49	72	21	egg	egg	NOUN
cvj-49	72	22	-	-	PUNCT
cvj-49	72	23	yolk	yolk	NOUN
cvj-49	72	24	found	find	VERB
cvj-49	72	25	in	in	ADP
cvj-49	72	26	semen	semen	NOUN
cvj-49	72	27	extenders	extender	NOUN
cvj-49	72	28	as	as	ADV
cvj-49	72	29	well	well	ADV
cvj-49	72	30	as	as	ADP
cvj-49	72	31	to	to	ADP
cvj-49	72	32	other	other	ADJ
cvj-49	72	33	fragments	fragment	NOUN
cvj-49	72	34	of	of	ADP
cvj-49	72	35	spermatozoa	spermatozoa	NOUN
cvj-49	72	36	[	[	X
cvj-49	72	37	10	10	NUM
cvj-49	72	38	]	]	PUNCT
cvj-49	72	39	.	.	PUNCT
cvj-49	73	1	to	to	PART
cvj-49	73	2	prepare	prepare	VERB
cvj-49	73	3	the	the	DET
cvj-49	73	4	stock	stock	NOUN
cvj-49	73	5	solution	solution	NOUN
cvj-49	73	6	,	,	PUNCT
cvj-49	73	7	lyophilized	lyophilize	VERB
cvj-49	73	8	pna	pna	NOUN
cvj-49	73	9	-	-	NOUN
cvj-49	73	10	fitc	fitc	NOUN
cvj-49	73	11	is	be	AUX
cvj-49	73	12	resuspended	resuspend	VERB
cvj-49	73	13	in	in	ADP
cvj-49	73	14	water	water	NOUN
cvj-49	73	15	to	to	ADP
cvj-49	73	16	a	a	DET
cvj-49	73	17	concentration	concentration	NOUN
cvj-49	73	18	of	of	ADP
cvj-49	73	19	0.1	0.1	NUM
cvj-49	73	20	-	-	SYM
cvj-49	73	21	1	1	NUM
cvj-49	73	22	mg	mg	NUM
cvj-49	73	23	/	/	SYM
cvj-49	73	24	ml	ml	NOUN
cvj-49	74	1	and	and	CCONJ
cvj-49	74	2	then	then	ADV
cvj-49	74	3	stored	store	VERB
cvj-49	74	4	frozen	frozen	ADJ
cvj-49	74	5	until	until	ADP
cvj-49	74	6	use	use	NOUN
cvj-49	74	7	.	.	PUNCT
cvj-49	75	1	the	the	DET
cvj-49	75	2	1	1	NUM
cvj-49	75	3	mg	mg	NUM
cvj-49	75	4	/	/	SYM
cvj-49	75	5	ml	ml	NOUN
cvj-49	75	6	solution	solution	NOUN
cvj-49	75	7	is	be	AUX
cvj-49	75	8	usually	usually	ADV
cvj-49	75	9	preferred	prefer	VERB
cvj-49	75	10	.	.	PUNCT
cvj-49	76	1	if	if	SCONJ
cvj-49	76	2	storage	storage	NOUN
cvj-49	76	3	at	at	ADP
cvj-49	76	4	the	the	DET
cvj-49	76	5	refrigeration	refrigeration	NOUN
cvj-49	76	6	temperature	temperature	NOUN
cvj-49	76	7	is	be	AUX
cvj-49	76	8	needed	need	VERB
cvj-49	76	9	,	,	PUNCT
cvj-49	76	10	a	a	DET
cvj-49	76	11	supplementation	supplementation	NOUN
cvj-49	76	12	with	with	ADP
cvj-49	76	13	2	2	NUM
cvj-49	76	14	mm	mm	INTJ
cvj-49	76	15	na	na	NOUN
cvj-49	76	16	-	-	PUNCT
cvj-49	76	17	azide	azide	NOUN
cvj-49	76	18	is	be	AUX
cvj-49	76	19	mandatory	mandatory	ADJ
cvj-49	76	20	.	.	PUNCT
cvj-49	77	1	the	the	DET
cvj-49	77	2	usual	usual	ADJ
cvj-49	77	3	protocol	protocol	NOUN
cvj-49	77	4	when	when	SCONJ
cvj-49	77	5	assessing	assess	VERB
cvj-49	77	6	the	the	DET
cvj-49	77	7	acrosome	acrosome	NOUN
cvj-49	77	8	reaction	reaction	NOUN
cvj-49	77	9	is	be	AUX
cvj-49	77	10	to	to	PART
cvj-49	77	11	combine	combine	VERB
cvj-49	77	12	pna	pna	NOUN
cvj-49	77	13	-	-	NOUN
cvj-49	77	14	fitc	fitc	NOUN
cvj-49	77	15	staining	stain	VERB
cvj-49	77	16	with	with	ADP
cvj-49	77	17	pi	pi	NOUN
cvj-49	77	18	,	,	PUNCT
cvj-49	77	19	in	in	ADP
cvj-49	77	20	order	order	NOUN
cvj-49	77	21	to	to	PART
cvj-49	77	22	also	also	ADV
cvj-49	77	23	observe	observe	VERB
cvj-49	77	24	the	the	DET
cvj-49	77	25	population	population	NOUN
cvj-49	77	26	of	of	ADP
cvj-49	77	27	non	non	ADJ
cvj-49	77	28	-	-	ADJ
cvj-49	77	29	viable	viable	ADJ
cvj-49	77	30	spermatozoa	spermatozoon	NOUN
cvj-49	77	31	.	.	PUNCT
cvj-49	78	1	the	the	DET
cvj-49	78	2	pi	pi	PROPN
cvj-49	78	3	stock	stock	NOUN
cvj-49	78	4	solution	solution	NOUN
cvj-49	78	5	can	can	AUX
cvj-49	78	6	also	also	ADV
cvj-49	78	7	be	be	AUX
cvj-49	78	8	stored	store	VERB
cvj-49	78	9	frozen	frozen	ADJ
cvj-49	78	10	,	,	PUNCT
cvj-49	78	11	at	at	ADP
cvj-49	78	12	a	a	DET
cvj-49	78	13	concentration	concentration	NOUN
cvj-49	78	14	between	between	ADP
cvj-49	78	15	50	50	NUM
cvj-49	78	16	µg-5mg	µg-5mg	NOUN
cvj-49	78	17	/	/	SYM
cvj-49	78	18	ml	ml	NOUN
cvj-49	78	19	,	,	PUNCT
cvj-49	78	20	but	but	CCONJ
cvj-49	78	21	most	most	ADV
cvj-49	78	22	frequently	frequently	ADV
cvj-49	78	23	a	a	DET
cvj-49	78	24	1	1	NUM
cvj-49	78	25	mg	mg	NUM
cvj-49	78	26	/	/	SYM
cvj-49	78	27	ml	ml	NOUN
cvj-49	78	28	solution	solution	NOUN
cvj-49	78	29	is	be	AUX
cvj-49	78	30	preferred	prefer	VERB
cvj-49	78	31	.	.	PUNCT
cvj-49	79	1	the	the	DET
cvj-49	79	2	working	work	VERB
cvj-49	79	3	solutions	solution	NOUN
cvj-49	79	4	can	can	AUX
cvj-49	79	5	easily	easily	ADV
cvj-49	79	6	be	be	AUX
cvj-49	79	7	prepared	prepare	VERB
cvj-49	79	8	based	base	VERB
cvj-49	79	9	on	on	ADP
cvj-49	79	10	the	the	DET
cvj-49	79	11	stock	stock	NOUN
cvj-49	79	12	solutions	solution	NOUN
cvj-49	79	13	at	at	ADP
cvj-49	79	14	1	1	NUM
cvj-49	79	15	mg	mg	NUM
cvj-49	79	16	/	/	SYM
cvj-49	79	17	ml	ml	NOUN
cvj-49	79	18	.	.	PUNCT
cvj-49	80	1	after	after	ADP
cvj-49	80	2	thawing	thaw	VERB
cvj-49	80	3	,	,	PUNCT
cvj-49	80	4	10	10	NUM
cvj-49	80	5	µl	µl	NOUN
cvj-49	80	6	of	of	ADP
cvj-49	80	7	both	both	CCONJ
cvj-49	80	8	the	the	DET
cvj-49	80	9	pna	pna	NOUN
cvj-49	80	10	-	-	NOUN
cvj-49	80	11	fitc	fitc	NOUN
cvj-49	80	12	and	and	CCONJ
cvj-49	80	13	pi	pi	NOUN
cvj-49	80	14	solutions	solution	NOUN
cvj-49	80	15	are	be	AUX
cvj-49	80	16	added	add	VERB
cvj-49	80	17	to	to	ADP
cvj-49	80	18	10	10	NUM
cvj-49	80	19	ml	ml	NOUN
cvj-49	80	20	of	of	ADP
cvj-49	80	21	pbs	pbs	PROPN
cvj-49	80	22	and	and	CCONJ
cvj-49	80	23	0.5	0.5	NUM
cvj-49	80	24	ml	ml	NOUN
cvj-49	80	25	of	of	ADP
cvj-49	80	26	the	the	DET
cvj-49	80	27	resulting	result	VERB
cvj-49	80	28	solution	solution	NOUN
cvj-49	80	29	are	be	AUX
cvj-49	80	30	used	use	VERB
cvj-49	80	31	to	to	PART
cvj-49	80	32	dilute	dilute	VERB
cvj-49	80	33	the	the	DET
cvj-49	80	34	semen	semen	NOUN
cvj-49	80	35	sample	sample	NOUN
cvj-49	80	36	to	to	ADP
cvj-49	80	37	a	a	DET
cvj-49	80	38	concentration	concentration	NOUN
cvj-49	80	39	of	of	ADP
cvj-49	80	40	1	1	NUM
cvj-49	80	41	-	-	SYM
cvj-49	80	42	2	2	NUM
cvj-49	80	43	x	x	SYM
cvj-49	80	44	106	106	NUM
cvj-49	80	45	spermatozoa	spermatozoon	NOUN
cvj-49	80	46	/	/	SYM
cvj-49	80	47	ml	ml	NOUN
cvj-49	80	48	.	.	PUNCT
cvj-49	81	1	the	the	DET
cvj-49	81	2	working	work	VERB
cvj-49	81	3	solution	solution	NOUN
cvj-49	81	4	should	should	AUX
cvj-49	81	5	be	be	AUX
cvj-49	81	6	kept	keep	VERB
cvj-49	81	7	in	in	ADP
cvj-49	81	8	the	the	DET
cvj-49	81	9	darkness	darkness	NOUN
cvj-49	81	10	until	until	ADP
cvj-49	81	11	use	use	NOUN
cvj-49	81	12	,	,	PUNCT
cvj-49	81	13	but	but	CCONJ
cvj-49	81	14	not	not	PART
cvj-49	81	15	more	more	ADJ
cvj-49	81	16	than	than	ADP
cvj-49	81	17	12	12	NUM
cvj-49	81	18	-	-	SYM
cvj-49	81	19	24	24	NUM
cvj-49	81	20	hours	hour	NOUN
cvj-49	81	21	.	.	PUNCT
cvj-49	82	1	after	after	ADP
cvj-49	82	2	staining	stain	VERB
cvj-49	82	3	,	,	PUNCT
cvj-49	82	4	spermatozoa	spermatozoon	NOUN
cvj-49	82	5	should	should	AUX
cvj-49	82	6	be	be	AUX
cvj-49	82	7	incubated	incubate	VERB
cvj-49	82	8	in	in	ADP
cvj-49	82	9	darkness	darkness	NOUN
cvj-49	82	10	for	for	ADP
cvj-49	82	11	15	15	NUM
cvj-49	82	12	minutes	minute	NOUN
cvj-49	82	13	at	at	ADP
cvj-49	82	14	37	37	NUM
cvj-49	82	15	°	°	NOUN
cvj-49	82	16	c	c	NOUN
cvj-49	82	17	.	.	PUNCT
cvj-49	83	1	next	next	ADV
cvj-49	83	2	,	,	PUNCT
cvj-49	83	3	the	the	DET
cvj-49	83	4	samples	sample	NOUN
cvj-49	83	5	are	be	AUX
cvj-49	83	6	run	run	VERB
cvj-49	83	7	in	in	ADP
cvj-49	83	8	the	the	DET
cvj-49	83	9	flow	flow	NOUN
cvj-49	83	10	cytometer	cytometer	NOUN
cvj-49	83	11	and	and	CCONJ
cvj-49	83	12	two	two	NUM
cvj-49	83	13	dot	dot	NOUN
cvj-49	83	14	plots	plot	NOUN
cvj-49	83	15	are	be	AUX
cvj-49	83	16	needed	need	VERB
cvj-49	83	17	:	:	PUNCT
cvj-49	83	18	one	one	NUM
cvj-49	83	19	for	for	ADP
cvj-49	83	20	spermatozoa	spermatozoa	NOUN
cvj-49	83	21	gating	gate	VERB
cvj-49	83	22	(	(	PUNCT
cvj-49	83	23	fsc	fsc	PROPN
cvj-49	83	24	-	-	PUNCT
cvj-49	83	25	h	h	PROPN
cvj-49	83	26	vs	vs	ADP
cvj-49	83	27	ssc	ssc	NOUN
cvj-49	83	28	-	-	PUNCT
cvj-49	83	29	h	h	NOUN
cvj-49	83	30	)	)	PUNCT
cvj-49	83	31	and	and	CCONJ
cvj-49	83	32	another	another	PRON
cvj-49	83	33	for	for	ADP
cvj-49	83	34	acrosome	acrosome	NOUN
cvj-49	83	35	reaction	reaction	NOUN
cvj-49	83	36	/	/	SYM
cvj-49	83	37	viability	viability	NOUN
cvj-49	83	38	(	(	PUNCT
cvj-49	83	39	fl1	fl1	ADV
cvj-49	83	40	-	-	PUNCT
cvj-49	83	41	h	h	NOUN
cvj-49	83	42	vs	vs	ADP
cvj-49	83	43	fl3	fl3	NOUN
cvj-49	83	44	-	-	PUNCT
cvj-49	83	45	h	h	NOUN
cvj-49	83	46	)	)	PUNCT
cvj-49	83	47	.	.	PUNCT
cvj-49	84	1	following	follow	VERB
cvj-49	84	2	analysis	analysis	NOUN
cvj-49	84	3	,	,	PUNCT
cvj-49	84	4	four	four	NUM
cvj-49	84	5	different	different	ADJ
cvj-49	84	6	populations	population	NOUN
cvj-49	84	7	of	of	ADP
cvj-49	84	8	spermatozoa	spermatozoa	NOUN
cvj-49	84	9	can	can	AUX
cvj-49	84	10	be	be	AUX
cvj-49	84	11	identified	identify	VERB
cvj-49	84	12	:	:	PUNCT
cvj-49	84	13	•	•	ADP
cvj-49	84	14	the	the	DET
cvj-49	84	15	pna	pna	NOUN
cvj-49	84	16	-	-	PUNCT
cvj-49	84	17	fitc–/pi	fitc–/pi	ADJ
cvj-49	84	18	–	–	PUNCT
cvj-49	84	19	population	population	NOUN
cvj-49	84	20	is	be	AUX
cvj-49	84	21	represented	represent	VERB
cvj-49	84	22	by	by	ADP
cvj-49	84	23	viable	viable	ADJ
cvj-49	84	24	spermatozoa	spermatozoon	NOUN
cvj-49	84	25	with	with	ADP
cvj-49	84	26	unreacted	unreacted	ADJ
cvj-49	84	27	acrosome	acrosome	NOUN
cvj-49	84	28	;	;	PUNCT
cvj-49	84	29	•	•	NUM
cvj-49	84	30	the	the	DET
cvj-49	84	31	pna	pna	NOUN
cvj-49	84	32	-	-	PUNCT
cvj-49	84	33	fitc+/pi	fitc+/pi	ADJ
cvj-49	84	34	–	–	PUNCT
cvj-49	84	35	population	population	NOUN
cvj-49	84	36	is	be	AUX
cvj-49	84	37	represented	represent	VERB
cvj-49	84	38	by	by	ADP
cvj-49	84	39	viable	viable	ADJ
cvj-49	84	40	spermatozoa	spermatozoon	NOUN
cvj-49	84	41	with	with	ADP
cvj-49	84	42	reacted	react	VERB
cvj-49	84	43	acrosome	acrosome	ADV
cvj-49	84	44	;	;	PUNCT
cvj-49	84	45	•	•	NUM
cvj-49	84	46	the	the	DET
cvj-49	84	47	pna	pna	NOUN
cvj-49	84	48	-	-	ADJ
cvj-49	84	49	fitc+/pi+	fitc+/pi+	ADJ
cvj-49	84	50	population	population	NOUN
cvj-49	84	51	is	be	AUX
cvj-49	84	52	represented	represent	VERB
cvj-49	84	53	by	by	ADP
cvj-49	84	54	moribund	moribund	ADJ
cvj-49	84	55	or	or	CCONJ
cvj-49	84	56	dead	dead	ADJ
cvj-49	84	57	spermatozoa	spermatozoon	NOUN
cvj-49	84	58	with	with	ADP
cvj-49	84	59	reacted	react	VERB
cvj-49	84	60	acrosome	acrosome	ADV
cvj-49	84	61	;	;	PUNCT
cvj-49	84	62	•	•	NUM
cvj-49	84	63	the	the	DET
cvj-49	84	64	pna	pna	NOUN
cvj-49	84	65	-	-	ADJ
cvj-49	84	66	fitc–/pi+	fitc–/pi+	ADJ
cvj-49	84	67	population	population	NOUN
cvj-49	84	68	is	be	AUX
cvj-49	84	69	represented	represent	VERB
cvj-49	84	70	by	by	ADP
cvj-49	84	71	moribund	moribund	ADJ
cvj-49	84	72	or	or	CCONJ
cvj-49	84	73	dead	dead	ADJ
cvj-49	84	74	spermatozoa	spermatozoon	NOUN
cvj-49	84	75	with	with	ADP
cvj-49	84	76	unreacted	unreacted	ADJ
cvj-49	84	77	acrosome	acrosome	NOUN
cvj-49	84	78	.	.	PUNCT
cvj-49	85	1	6	6	NUM
cvj-49	85	2	.	.	X
cvj-49	85	3	evaluation	evaluation	NOUN
cvj-49	85	4	of	of	ADP
cvj-49	85	5	mitochondrial	mitochondrial	ADJ
cvj-49	85	6	activity	activity	NOUN
cvj-49	85	7	in	in	ADP
cvj-49	85	8	mammalian	mammalian	ADJ
cvj-49	85	9	spermatozoa	spermatozoon	NOUN
cvj-49	85	10	,	,	PUNCT
cvj-49	85	11	mitochondria	mitochondria	PROPN
cvj-49	85	12	are	be	AUX
cvj-49	85	13	essential	essential	ADJ
cvj-49	85	14	organelles	organelle	NOUN
cvj-49	85	15	which	which	PRON
cvj-49	85	16	play	play	VERB
cvj-49	85	17	a	a	DET
cvj-49	85	18	crucial	crucial	ADJ
cvj-49	85	19	role	role	NOUN
cvj-49	85	20	for	for	ADP
cvj-49	85	21	their	their	PRON
cvj-49	85	22	motility	motility	NOUN
cvj-49	85	23	and	and	CCONJ
cvj-49	85	24	fertilizing	fertilizing	NOUN
cvj-49	85	25	capability	capability	NOUN
cvj-49	85	26	,	,	PUNCT
cvj-49	85	27	by	by	ADP
cvj-49	85	28	contributing	contribute	VERB
cvj-49	85	29	to	to	ADP
cvj-49	85	30	atp	atp	PROPN
cvj-49	85	31	and	and	CCONJ
cvj-49	85	32	reactive	reactive	ADJ
cvj-49	85	33	oxygen	oxygen	NOUN
cvj-49	85	34	species	specie	NOUN
cvj-49	85	35	production	production	NOUN
cvj-49	85	36	as	as	ADV
cvj-49	85	37	well	well	ADV
cvj-49	85	38	cluj	cluj	NOUN
cvj-49	85	39	vet	vet	NOUN
cvj-49	85	40	j	j	PROPN
cvj-49	85	41	2023	2023	NUM
cvj-49	85	42	,	,	PUNCT
cvj-49	85	43	vol	vol	NOUN
cvj-49	85	44	28	28	NUM
cvj-49	85	45	,	,	PUNCT
cvj-49	85	46	issue	issue	NOUN
cvj-49	85	47	47	47	NUM
cvj-49	85	48	as	as	ADP
cvj-49	85	49	calcium	calcium	NOUN
cvj-49	85	50	level	level	NOUN
cvj-49	85	51	control	control	NOUN
cvj-49	85	52	.	.	PUNCT
cvj-49	86	1	their	their	PRON
cvj-49	86	2	integrity	integrity	NOUN
cvj-49	86	3	and	and	CCONJ
cvj-49	86	4	activity	activity	NOUN
cvj-49	86	5	can	can	AUX
cvj-49	86	6	be	be	AUX
cvj-49	86	7	assessed	assess	VERB
cvj-49	86	8	by	by	ADP
cvj-49	86	9	quantitatively	quantitatively	ADV
cvj-49	86	10	evaluating	evaluate	VERB
cvj-49	86	11	their	their	PRON
cvj-49	86	12	membrane	membrane	NOUN
cvj-49	86	13	potential	potential	NOUN
cvj-49	86	14	,	,	PUNCT
cvj-49	86	15	using	use	VERB
cvj-49	86	16	a	a	DET
cvj-49	86	17	fluorescent	fluorescent	ADJ
cvj-49	86	18	dye	dye	NOUN
cvj-49	86	19	called	call	VERB
cvj-49	86	20	5,5,6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimi	5,5,6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimi	NUM
cvj-49	86	21	-	-	PUNCT
cvj-49	86	22	dazoylcarbocyanine	dazoylcarbocyanine	ADJ
cvj-49	86	23	iodide	iodide	NOUN
cvj-49	86	24	(	(	PUNCT
cvj-49	86	25	jc-1	jc-1	NOUN
cvj-49	86	26	)	)	PUNCT
cvj-49	86	27	.	.	PUNCT
cvj-49	87	1	when	when	SCONJ
cvj-49	87	2	mitochondrial	mitochondrial	ADJ
cvj-49	87	3	membrane	membrane	NOUN
cvj-49	87	4	potential	potential	NOUN
cvj-49	87	5	is	be	AUX
cvj-49	87	6	high	high	ADJ
cvj-49	87	7	,	,	PUNCT
cvj-49	87	8	jc-1	jc-1	NOUN
cvj-49	87	9	forms	form	VERB
cvj-49	87	10	combinations	combination	NOUN
cvj-49	87	11	that	that	PRON
cvj-49	87	12	produce	produce	VERB
cvj-49	87	13	red	red	ADJ
cvj-49	87	14	fluorescence	fluorescence	NOUN
cvj-49	87	15	,	,	PUNCT
cvj-49	87	16	while	while	SCONJ
cvj-49	87	17	in	in	ADP
cvj-49	87	18	the	the	DET
cvj-49	87	19	case	case	NOUN
cvj-49	87	20	of	of	ADP
cvj-49	87	21	low	low	ADJ
cvj-49	87	22	mitochondrial	mitochondrial	ADJ
cvj-49	87	23	membrane	membrane	NOUN
cvj-49	87	24	potential	potential	NOUN
cvj-49	87	25	,	,	PUNCT
cvj-49	87	26	jc-1	jc-1	NOUN
cvj-49	87	27	stays	stay	VERB
cvj-49	87	28	monomeric	monomeric	ADJ
cvj-49	87	29	and	and	CCONJ
cvj-49	87	30	emits	emit	VERB
cvj-49	87	31	green	green	ADJ
cvj-49	87	32	fluorescence	fluorescence	NOUN
cvj-49	87	33	(	(	PUNCT
cvj-49	87	34	11	11	NUM
cvj-49	87	35	)	)	PUNCT
cvj-49	87	36	.	.	PUNCT
cvj-49	88	1	the	the	DET
cvj-49	88	2	jc-1	jc-1	NOUN
cvj-49	88	3	stock	stock	NOUN
cvj-49	88	4	solution	solution	NOUN
cvj-49	88	5	can	can	AUX
cvj-49	88	6	be	be	AUX
cvj-49	88	7	prepared	prepare	VERB
cvj-49	88	8	by	by	ADP
cvj-49	88	9	resuspending	resuspend	VERB
cvj-49	88	10	the	the	DET
cvj-49	88	11	lyophilized	lyophilized	ADJ
cvj-49	88	12	powder	powder	NOUN
cvj-49	88	13	in	in	ADP
cvj-49	88	14	dmso	dmso	NOUN
cvj-49	88	15	,	,	PUNCT
cvj-49	88	16	to	to	ADP
cvj-49	88	17	a	a	DET
cvj-49	88	18	concentration	concentration	NOUN
cvj-49	88	19	of	of	ADP
cvj-49	88	20	2	2	NUM
cvj-49	88	21	mg	mg	NUM
cvj-49	88	22	/	/	SYM
cvj-49	88	23	ml	ml	PROPN
cvj-49	88	24	(	(	PUNCT
cvj-49	88	25	3	3	NUM
cvj-49	88	26	mm	mm	NOUN
cvj-49	88	27	)	)	PUNCT
cvj-49	88	28	and	and	CCONJ
cvj-49	88	29	stored	store	VERB
cvj-49	88	30	frozen	frozen	ADJ
cvj-49	88	31	in	in	ADP
cvj-49	88	32	a	a	DET
cvj-49	88	33	dark	dark	ADJ
cvj-49	88	34	vial	vial	NOUN
cvj-49	88	35	.	.	PUNCT
cvj-49	89	1	the	the	DET
cvj-49	89	2	working	work	VERB
cvj-49	89	3	solution	solution	NOUN
cvj-49	89	4	is	be	AUX
cvj-49	89	5	a	a	DET
cvj-49	89	6	1000	1000	NUM
cvj-49	89	7	fold	fold	ADJ
cvj-49	89	8	dilution	dilution	NOUN
cvj-49	89	9	of	of	ADP
cvj-49	89	10	the	the	DET
cvj-49	89	11	stock	stock	NOUN
cvj-49	89	12	solution	solution	NOUN
cvj-49	89	13	.	.	PUNCT
cvj-49	90	1	therefore	therefore	ADV
cvj-49	90	2	,	,	PUNCT
cvj-49	90	3	10	10	NUM
cvj-49	90	4	µl	µl	NOUN
cvj-49	90	5	of	of	ADP
cvj-49	90	6	the	the	DET
cvj-49	90	7	stock	stock	NOUN
cvj-49	90	8	solution	solution	NOUN
cvj-49	90	9	should	should	AUX
cvj-49	90	10	be	be	AUX
cvj-49	90	11	added	add	VERB
cvj-49	90	12	to	to	ADP
cvj-49	90	13	10	10	NUM
cvj-49	90	14	ml	ml	NOUN
cvj-49	90	15	of	of	ADP
cvj-49	90	16	pbs	pbs	PROPN
cvj-49	90	17	and	and	CCONJ
cvj-49	90	18	0.5	0.5	NUM
cvj-49	90	19	ml	ml	NOUN
cvj-49	90	20	of	of	ADP
cvj-49	90	21	the	the	DET
cvj-49	90	22	resulting	result	VERB
cvj-49	90	23	solution	solution	NOUN
cvj-49	90	24	are	be	AUX
cvj-49	90	25	used	use	VERB
cvj-49	90	26	to	to	PART
cvj-49	90	27	dilute	dilute	VERB
cvj-49	90	28	the	the	DET
cvj-49	90	29	semen	semen	NOUN
cvj-49	90	30	sample	sample	NOUN
cvj-49	90	31	to	to	ADP
cvj-49	90	32	a	a	DET
cvj-49	90	33	concentration	concentration	NOUN
cvj-49	90	34	of	of	ADP
cvj-49	90	35	1	1	NUM
cvj-49	90	36	-	-	SYM
cvj-49	90	37	2	2	NUM
cvj-49	90	38	x	x	SYM
cvj-49	90	39	106	106	NUM
cvj-49	90	40	spermatozoa	spermatozoon	NOUN
cvj-49	90	41	/	/	SYM
cvj-49	90	42	ml	ml	NOUN
cvj-49	90	43	.	.	PUNCT
cvj-49	91	1	following	follow	VERB
cvj-49	91	2	incubation	incubation	NOUN
cvj-49	91	3	for	for	ADP
cvj-49	91	4	15	15	NUM
cvj-49	91	5	-	-	SYM
cvj-49	91	6	20	20	NUM
cvj-49	91	7	minutes	minute	NOUN
cvj-49	91	8	in	in	ADP
cvj-49	91	9	darkness	darkness	NOUN
cvj-49	91	10	at	at	ADP
cvj-49	91	11	37	37	NUM
cvj-49	91	12	°	°	NOUN
cvj-49	91	13	c	c	NOUN
cvj-49	91	14	,	,	PUNCT
cvj-49	91	15	samples	sample	NOUN
cvj-49	91	16	are	be	AUX
cvj-49	91	17	run	run	VERB
cvj-49	91	18	in	in	ADP
cvj-49	91	19	the	the	DET
cvj-49	91	20	flow	flow	NOUN
cvj-49	91	21	cytometer	cytometer	NOUN
cvj-49	91	22	and	and	CCONJ
cvj-49	91	23	visualized	visualize	VERB
cvj-49	91	24	in	in	ADP
cvj-49	91	25	two	two	NUM
cvj-49	91	26	dot	dot	NOUN
cvj-49	91	27	plots	plot	NOUN
cvj-49	91	28	:	:	PUNCT
cvj-49	91	29	one	one	NUM
cvj-49	91	30	for	for	ADP
cvj-49	91	31	gating	gate	VERB
cvj-49	91	32	the	the	DET
cvj-49	91	33	spermatozoa	spermatozoon	NOUN
cvj-49	91	34	(	(	PUNCT
cvj-49	91	35	the	the	DET
cvj-49	91	36	usual	usual	ADJ
cvj-49	91	37	fsc	fsc	PROPN
cvj-49	91	38	-	-	PUNCT
cvj-49	91	39	h	h	PROPN
cvj-49	91	40	vs	vs	ADP
cvj-49	91	41	ssc	ssc	NOUN
cvj-49	91	42	-	-	PUNCT
cvj-49	91	43	h	h	NOUN
cvj-49	91	44	)	)	PUNCT
cvj-49	91	45	and	and	CCONJ
cvj-49	91	46	another	another	PRON
cvj-49	91	47	for	for	ADP
cvj-49	91	48	mitochondrial	mitochondrial	ADJ
cvj-49	91	49	membrane	membrane	NOUN
cvj-49	91	50	potential	potential	NOUN
cvj-49	91	51	(	(	PUNCT
cvj-49	91	52	fl1	fl1	ADV
cvj-49	91	53	-	-	PUNCT
cvj-49	91	54	h	h	NOUN
cvj-49	91	55	vs	vs	ADP
cvj-49	91	56	fl2	fl2	PROPN
cvj-49	91	57	-	-	PUNCT
cvj-49	91	58	h	h	NOUN
cvj-49	91	59	)	)	PUNCT
cvj-49	91	60	.	.	PUNCT
cvj-49	92	1	analysis	analysis	NOUN
cvj-49	92	2	of	of	ADP
cvj-49	92	3	dot	dot	NOUN
cvj-49	92	4	plots	plot	NOUN
cvj-49	92	5	allows	allow	VERB
cvj-49	92	6	classification	classification	NOUN
cvj-49	92	7	of	of	ADP
cvj-49	92	8	ejaculates	ejaculate	NOUN
cvj-49	92	9	according	accord	VERB
cvj-49	92	10	to	to	ADP
cvj-49	92	11	mitochondrial	mitochondrial	ADJ
cvj-49	92	12	activity	activity	NOUN
cvj-49	92	13	of	of	ADP
cvj-49	92	14	spermatozoa	spermatozoon	NOUN
cvj-49	92	15	,	,	PUNCT
cvj-49	92	16	as	as	SCONJ
cvj-49	92	17	follows	follow	VERB
cvj-49	92	18	:	:	PUNCT
cvj-49	92	19	•	•	NOUN
cvj-49	92	20	spermatozoa	spermatozoon	NOUN
cvj-49	92	21	with	with	ADP
cvj-49	92	22	high	high	ADJ
cvj-49	92	23	fl1	fl1	ADJ
cvj-49	92	24	-	-	PUNCT
cvj-49	92	25	h	h	NOUN
cvj-49	92	26	and	and	CCONJ
cvj-49	92	27	low	low	ADJ
cvj-49	92	28	fl2	fl2	PROPN
cvj-49	92	29	-	-	PUNCT
cvj-49	92	30	h	h	NOUN
cvj-49	92	31	have	have	VERB
cvj-49	92	32	a	a	DET
cvj-49	92	33	low	low	ADJ
cvj-49	92	34	mitochondrial	mitochondrial	ADJ
cvj-49	92	35	membrane	membrane	NOUN
cvj-49	92	36	potential	potential	NOUN
cvj-49	92	37	and	and	CCONJ
cvj-49	92	38	are	be	AUX
cvj-49	92	39	considered	consider	VERB
cvj-49	92	40	of	of	ADP
cvj-49	92	41	low	low	ADJ
cvj-49	92	42	fertilizing	fertilizing	NOUN
cvj-49	92	43	ability	ability	NOUN
cvj-49	92	44	;	;	PUNCT
cvj-49	92	45	•	•	NUM
cvj-49	92	46	spermatozoa	spermatozoon	NOUN
cvj-49	92	47	with	with	ADP
cvj-49	92	48	low	low	ADJ
cvj-49	92	49	fl1	fl1	ADJ
cvj-49	92	50	-	-	PUNCT
cvj-49	92	51	h	h	NOUN
cvj-49	92	52	and	and	CCONJ
cvj-49	92	53	high	high	ADJ
cvj-49	92	54	fl2	fl2	PROPN
cvj-49	92	55	-	-	PUNCT
cvj-49	92	56	h	h	NOUN
cvj-49	92	57	have	have	VERB
cvj-49	92	58	high	high	ADJ
cvj-49	92	59	mitochondrial	mitochondrial	ADJ
cvj-49	92	60	membrane	membrane	NOUN
cvj-49	92	61	potential	potential	NOUN
cvj-49	92	62	and	and	CCONJ
cvj-49	92	63	potentially	potentially	ADV
cvj-49	92	64	poses	pose	VERB
cvj-49	92	65	a	a	DET
cvj-49	92	66	fertilizing	fertilize	VERB
cvj-49	92	67	ability	ability	NOUN
cvj-49	92	68	(	(	PUNCT
cvj-49	92	69	this	this	DET
cvj-49	92	70	category	category	NOUN
cvj-49	92	71	should	should	AUX
cvj-49	92	72	be	be	AUX
cvj-49	92	73	the	the	DET
cvj-49	92	74	most	most	ADV
cvj-49	92	75	abundant	abundant	ADJ
cvj-49	92	76	in	in	ADP
cvj-49	92	77	good	good	ADJ
cvj-49	92	78	quality	quality	NOUN
cvj-49	92	79	ejaculates	ejaculate	NOUN
cvj-49	92	80	)	)	PUNCT
cvj-49	92	81	;	;	PUNCT
cvj-49	92	82	•	•	NUM
cvj-49	92	83	spermatozoa	spermatozoon	NOUN
cvj-49	92	84	with	with	ADP
cvj-49	92	85	high	high	ADJ
cvj-49	92	86	fl1	fl1	ADJ
cvj-49	92	87	-	-	PUNCT
cvj-49	92	88	h	h	NOUN
cvj-49	92	89	and	and	CCONJ
cvj-49	92	90	high	high	ADJ
cvj-49	92	91	or	or	CCONJ
cvj-49	92	92	moderate	moderate	ADJ
cvj-49	92	93	fl2	fl2	NOUN
cvj-49	92	94	-	-	PUNCT
cvj-49	92	95	h	h	NOUN
cvj-49	92	96	are	be	AUX
cvj-49	92	97	considered	consider	VERB
cvj-49	92	98	to	to	PART
cvj-49	92	99	have	have	VERB
cvj-49	92	100	large	large	ADJ
cvj-49	92	101	gaps	gap	NOUN
cvj-49	92	102	in	in	ADP
cvj-49	92	103	their	their	PRON
cvj-49	92	104	mitochondria	mitochondria	NOUN
cvj-49	92	105	and	and	CCONJ
cvj-49	92	106	are	be	AUX
cvj-49	92	107	therefore	therefore	ADV
cvj-49	92	108	of	of	ADP
cvj-49	92	109	lower	low	ADJ
cvj-49	92	110	quality	quality	NOUN
cvj-49	92	111	;	;	PUNCT
cvj-49	92	112	•	•	NUM
cvj-49	92	113	spermatozoa	spermatozoon	NOUN
cvj-49	92	114	with	with	ADP
cvj-49	92	115	low	low	ADJ
cvj-49	92	116	fl1	fl1	ADJ
cvj-49	92	117	-	-	PUNCT
cvj-49	92	118	h	h	NOUN
cvj-49	92	119	and	and	CCONJ
cvj-49	92	120	low	low	ADJ
cvj-49	92	121	fl2	fl2	NOUN
cvj-49	92	122	-	-	PUNCT
cvj-49	92	123	h	h	NOUN
cvj-49	92	124	are	be	AUX
cvj-49	92	125	likely	likely	ADV
cvj-49	92	126	dead	dead	ADJ
cvj-49	92	127	and	and	CCONJ
cvj-49	92	128	have	have	VERB
cvj-49	92	129	a	a	DET
cvj-49	92	130	damaged	damage	VERB
cvj-49	92	131	midpiece	midpiece	NOUN
cvj-49	92	132	.	.	PUNCT
cvj-49	93	1	7	7	X
cvj-49	93	2	.	.	X
cvj-49	93	3	conclusions	conclusion	NOUN
cvj-49	93	4	flow	flow	NOUN
cvj-49	93	5	cytometry	cytometry	NOUN
cvj-49	93	6	is	be	AUX
cvj-49	93	7	an	an	DET
cvj-49	93	8	extremely	extremely	ADV
cvj-49	93	9	useful	useful	ADJ
cvj-49	93	10	and	and	CCONJ
cvj-49	93	11	powerful	powerful	ADJ
cvj-49	93	12	tool	tool	NOUN
cvj-49	93	13	that	that	PRON
cvj-49	93	14	can	can	AUX
cvj-49	93	15	be	be	AUX
cvj-49	93	16	used	use	VERB
cvj-49	93	17	for	for	ADP
cvj-49	93	18	advanced	advanced	ADJ
cvj-49	93	19	semen	semen	NOUN
cvj-49	93	20	analysis	analysis	NOUN
cvj-49	93	21	as	as	SCONJ
cvj-49	93	22	it	it	PRON
cvj-49	93	23	provides	provide	VERB
cvj-49	93	24	quick	quick	ADJ
cvj-49	93	25	and	and	CCONJ
cvj-49	93	26	reliable	reliable	ADJ
cvj-49	93	27	results	result	NOUN
cvj-49	93	28	,	,	PUNCT
cvj-49	93	29	enabling	enable	VERB
cvj-49	93	30	an	an	DET
cvj-49	93	31	accurate	accurate	ADJ
cvj-49	93	32	estimation	estimation	NOUN
cvj-49	93	33	of	of	ADP
cvj-49	93	34	various	various	ADJ
cvj-49	93	35	semen	semen	NOUN
cvj-49	93	36	parameters	parameter	NOUN
cvj-49	93	37	.	.	PUNCT
cvj-49	94	1	together	together	ADV
cvj-49	94	2	with	with	ADP
cvj-49	94	3	computer	computer	NOUN
cvj-49	94	4	assisted	assist	VERB
cvj-49	94	5	sperm	sperm	NOUN
cvj-49	94	6	analysis	analysis	NOUN
cvj-49	94	7	casa	casa	PROPN
cvj-49	94	8	systems	system	NOUN
cvj-49	94	9	,	,	PUNCT
cvj-49	94	10	flow	flow	NOUN
cvj-49	94	11	cytometers	cytometer	NOUN
cvj-49	94	12	allow	allow	VERB
cvj-49	94	13	an	an	DET
cvj-49	94	14	in	in	ADP
cvj-49	94	15	-	-	PUNCT
cvj-49	94	16	depth	depth	NOUN
cvj-49	94	17	analysis	analysis	NOUN
cvj-49	94	18	of	of	ADP
cvj-49	94	19	fresh	fresh	ADJ
cvj-49	94	20	,	,	PUNCT
cvj-49	94	21	chilled	chilled	ADJ
cvj-49	94	22	or	or	CCONJ
cvj-49	94	23	frozen	frozen	ADJ
cvj-49	94	24	/	/	SYM
cvj-49	94	25	thawed	thaw	VERB
cvj-49	94	26	semen	semen	NOUN
cvj-49	94	27	,	,	PUNCT
cvj-49	94	28	which	which	PRON
cvj-49	94	29	is	be	AUX
cvj-49	94	30	currently	currently	ADV
cvj-49	94	31	essential	essential	ADJ
cvj-49	94	32	for	for	ADP
cvj-49	94	33	research	research	NOUN
cvj-49	94	34	purposes	purpose	NOUN
cvj-49	94	35	,	,	PUNCT
cvj-49	94	36	but	but	CCONJ
cvj-49	94	37	also	also	ADV
cvj-49	94	38	for	for	ADP
cvj-49	94	39	diagnosis	diagnosis	NOUN
cvj-49	94	40	of	of	ADP
cvj-49	94	41	various	various	ADJ
cvj-49	94	42	male	male	NOUN
cvj-49	94	43	-	-	PUNCT
cvj-49	94	44	related	relate	VERB
cvj-49	94	45	fertility	fertility	NOUN
cvj-49	94	46	disorders	disorder	NOUN
cvj-49	94	47	,	,	PUNCT
cvj-49	94	48	both	both	CCONJ
cvj-49	94	49	in	in	ADP
cvj-49	94	50	veterinary	veterinary	ADJ
cvj-49	94	51	and	and	CCONJ
cvj-49	94	52	human	human	ADJ
cvj-49	94	53	medicine	medicine	NOUN
cvj-49	94	54	.	.	PUNCT
cvj-49	95	1	the	the	DET
cvj-49	95	2	equipment	equipment	NOUN
cvj-49	95	3	required	require	VERB
cvj-49	95	4	is	be	AUX
cvj-49	95	5	indeed	indeed	ADV
cvj-49	95	6	quite	quite	ADV
cvj-49	95	7	expensive	expensive	ADJ
cvj-49	95	8	and	and	CCONJ
cvj-49	95	9	the	the	DET
cvj-49	95	10	operators	operator	NOUN
cvj-49	95	11	need	need	VERB
cvj-49	95	12	special	special	ADJ
cvj-49	95	13	training	training	NOUN
cvj-49	95	14	,	,	PUNCT
cvj-49	95	15	while	while	SCONJ
cvj-49	95	16	experience	experience	NOUN
cvj-49	95	17	is	be	AUX
cvj-49	95	18	also	also	ADV
cvj-49	95	19	an	an	DET
cvj-49	95	20	asset	asset	NOUN
cvj-49	95	21	.	.	PUNCT
cvj-49	96	1	author	author	NOUN
cvj-49	96	2	contributions	contribution	NOUN
cvj-49	96	3	:	:	PUNCT
cvj-49	96	4	dc	dc	PROPN
cvj-49	96	5	,	,	PUNCT
cvj-49	96	6	bț	bț	PROPN
cvj-49	96	7	,	,	PUNCT
cvj-49	96	8	vt	vt	PROPN
cvj-49	96	9	and	and	CCONJ
cvj-49	96	10	mc	mc	PROPN
cvj-49	96	11	:	:	PUNCT
cvj-49	96	12	writing	writing	NOUN
cvj-49	96	13	—	—	PUNCT
cvj-49	96	14	original	original	ADJ
cvj-49	96	15	draft	draft	NOUN
cvj-49	96	16	preparation	preparation	NOUN
cvj-49	96	17	,	,	PUNCT
cvj-49	96	18	jtb	jtb	PROPN
cvj-49	96	19	and	and	CCONJ
cvj-49	96	20	vg	vg	PROPN
cvj-49	96	21	:	:	PUNCT
cvj-49	96	22	writing	writing	NOUN
cvj-49	96	23	—	—	PUNCT
cvj-49	96	24	review	review	NOUN
cvj-49	96	25	and	and	CCONJ
cvj-49	96	26	editing	editing	NOUN
cvj-49	96	27	.	.	PUNCT
cvj-49	97	1	all	all	DET
cvj-49	97	2	authors	author	NOUN
cvj-49	97	3	have	have	AUX
cvj-49	97	4	read	read	VERB
cvj-49	97	5	and	and	CCONJ
cvj-49	97	6	agreed	agree	VERB
cvj-49	97	7	to	to	ADP
cvj-49	97	8	the	the	DET
cvj-49	97	9	published	publish	VERB
cvj-49	97	10	version	version	NOUN
cvj-49	97	11	of	of	ADP
cvj-49	97	12	the	the	DET
cvj-49	97	13	manuscript	manuscript	NOUN
cvj-49	97	14	”	"	PUNCT
cvj-49	97	15	.	.	PUNCT
cvj-49	98	1	funding	funding	NOUN
cvj-49	98	2	:	:	PUNCT
cvj-49	98	3	dc	dc	PROPN
cvj-49	98	4	,	,	PUNCT
cvj-49	98	5	jtb	jtb	PROPN
cvj-49	98	6	,	,	PUNCT
cvj-49	98	7	vg	vg	PROPN
cvj-49	98	8	bț	bț	PROPN
cvj-49	98	9	,	,	PUNCT
cvj-49	98	10	vt	vt	PROPN
cvj-49	98	11	and	and	CCONJ
cvj-49	98	12	mc	mc	PROPN
cvj-49	98	13	were	be	AUX
cvj-49	98	14	funded	fund	VERB
cvj-49	98	15	by	by	ADP
cvj-49	98	16	an	an	DET
cvj-49	98	17	international	international	ADJ
cvj-49	98	18	collaborative	collaborative	ADJ
cvj-49	98	19	grant	grant	NOUN
cvj-49	98	20	of	of	ADP
cvj-49	98	21	the	the	DET
cvj-49	98	22	european	european	ADJ
cvj-49	98	23	economic	economic	ADJ
cvj-49	98	24	space	space	NOUN
cvj-49	98	25	between	between	ADP
cvj-49	98	26	romania	romania	PROPN
cvj-49	98	27	,	,	PUNCT
cvj-49	98	28	iceland	iceland	PROPN
cvj-49	98	29	,	,	PUNCT
cvj-49	98	30	and	and	CCONJ
cvj-49	98	31	norway	norway	PROPN
cvj-49	98	32	2014–2021	2014–2021	NUM
cvj-49	98	33	:	:	PUNCT
cvj-49	98	34	“	"	PUNCT
cvj-49	98	35	continuous	continuous	ADJ
cvj-49	98	36	flow	flow	NOUN
cvj-49	98	37	interchange	interchange	NOUN
cvj-49	98	38	of	of	ADP
cvj-49	98	39	communication	communication	NOUN
cvj-49	98	40	and	and	CCONJ
cvj-49	98	41	knowledge	knowledge	NOUN
cvj-49	98	42	in	in	ADP
cvj-49	98	43	biomedical	biomedical	ADJ
cvj-49	98	44	university	university	NOUN
cvj-49	98	45	research	research	NOUN
cvj-49	98	46	—	—	PUNCT
cvj-49	98	47	flow	flow	NOUN
cvj-49	98	48	”	"	PUNCT
cvj-49	98	49	,	,	PUNCT
cvj-49	98	50	no	no	INTJ
cvj-49	98	51	.	.	NOUN
cvj-49	98	52	21	21	NUM
cvj-49	98	53	-	-	PUNCT
cvj-49	98	54	cop-0034	cop-0034	NOUN
cvj-49	98	55	.	.	PUNCT
cvj-49	99	1	conflicts	conflict	NOUN
cvj-49	99	2	of	of	ADP
cvj-49	99	3	interest	interest	NOUN
cvj-49	99	4	:	:	PUNCT
cvj-49	99	5	the	the	DET
cvj-49	99	6	authors	author	NOUN
cvj-49	99	7	declare	declare	VERB
cvj-49	99	8	no	no	DET
cvj-49	99	9	conflict	conflict	NOUN
cvj-49	99	10	of	of	ADP
cvj-49	99	11	interest	interest	NOUN
cvj-49	99	12	.	.	PUNCT
cvj-49	100	1	references	reference	NOUN
cvj-49	100	2	1	1	NUM
cvj-49	100	3	.	.	PUNCT
cvj-49	100	4	petrunkina	petrunkina	NOUN
cvj-49	100	5	,	,	PUNCT
cvj-49	100	6	a.m.	a.m.	ADV
cvj-49	100	7	;	;	PUNCT
cvj-49	100	8	harrison	harrison	PROPN
cvj-49	100	9	,	,	PUNCT
cvj-49	100	10	r.a	r.a	PROPN
cvj-49	100	11	.	.	PROPN
cvj-49	100	12	cytometric	cytometric	ADJ
cvj-49	100	13	solutions	solution	NOUN
cvj-49	100	14	in	in	ADP
cvj-49	100	15	veterinary	veterinary	ADJ
cvj-49	100	16	andrology	andrology	NOUN
cvj-49	100	17	:	:	PUNCT
cvj-49	100	18	developments	development	NOUN
cvj-49	100	19	,	,	PUNCT
cvj-49	100	20	advantages	advantage	NOUN
cvj-49	100	21	,	,	PUNCT
cvj-49	100	22	and	and	CCONJ
cvj-49	100	23	limitations	limitation	NOUN
cvj-49	100	24	.	.	PUNCT
cvj-49	101	1	cytometry	cytometry	NOUN
cvj-49	101	2	a	a	DET
cvj-49	101	3	2011	2011	NUM
cvj-49	101	4	,	,	PUNCT
cvj-49	101	5	79	79	NUM
cvj-49	101	6	,	,	PUNCT
cvj-49	101	7	338–348	338–348	NUM
cvj-49	101	8	.	.	NOUN
cvj-49	102	1	2	2	X
cvj-49	102	2	.	.	X
cvj-49	102	3	peña	peña	PROPN
cvj-49	102	4	,	,	PUNCT
cvj-49	102	5	a.i	a.i	PROPN
cvj-49	102	6	.	.	PROPN
cvj-49	102	7	;	;	PUNCT
cvj-49	102	8	johannisson	johannisson	PROPN
cvj-49	102	9	,	,	PUNCT
cvj-49	102	10	a.	a.	NOUN
cvj-49	102	11	;	;	PUNCT
cvj-49	102	12	linde	linde	PROPN
cvj-49	102	13	-	-	PUNCT
cvj-49	102	14	forsberg	forsberg	PROPN
cvj-49	102	15	,	,	PUNCT
cvj-49	102	16	c.	c.	PROPN
cvj-49	102	17	validation	validation	NOUN
cvj-49	102	18	of	of	ADP
cvj-49	102	19	flow	flow	NOUN
cvj-49	102	20	cytometry	cytometry	NOUN
cvj-49	102	21	for	for	ADP
cvj-49	102	22	assessment	assessment	NOUN
cvj-49	102	23	of	of	ADP
cvj-49	102	24	viability	viability	NOUN
cvj-49	102	25	and	and	CCONJ
cvj-49	102	26	acrosomal	acrosomal	NOUN
cvj-49	102	27	integrity	integrity	NOUN
cvj-49	102	28	of	of	ADP
cvj-49	102	29	dog	dog	NOUN
cvj-49	102	30	spermatozoa	spermatozoon	NOUN
cvj-49	102	31	and	and	CCONJ
cvj-49	102	32	for	for	ADP
cvj-49	102	33	evaluation	evaluation	NOUN
cvj-49	102	34	of	of	ADP
cvj-49	102	35	different	different	ADJ
cvj-49	102	36	methods	method	NOUN
cvj-49	102	37	of	of	ADP
cvj-49	102	38	cryopreservation	cryopreservation	NOUN
cvj-49	102	39	.	.	PUNCT
cvj-49	103	1	j	j	PROPN
cvj-49	103	2	reprod	reprod	PROPN
cvj-49	103	3	fertil	fertil	PROPN
cvj-49	103	4	2001	2001	NUM
cvj-49	103	5	,	,	PUNCT
cvj-49	103	6	suppl	suppl	PROPN
cvj-49	103	7	57	57	NUM
cvj-49	103	8	,	,	PUNCT
cvj-49	103	9	371	371	NUM
cvj-49	103	10	-	-	SYM
cvj-49	103	11	376	376	NUM
cvj-49	103	12	.	.	PUNCT
cvj-49	104	1	3	3	X
cvj-49	104	2	.	.	X
cvj-49	104	3	januskauskas	januskauskas	PROPN
cvj-49	104	4	,	,	PUNCT
cvj-49	104	5	a.	a.	NOUN
cvj-49	104	6	;	;	PUNCT
cvj-49	104	7	johannisson	johannisson	PROPN
cvj-49	104	8	,	,	PUNCT
cvj-49	104	9	a.	a.	NOUN
cvj-49	104	10	;	;	PUNCT
cvj-49	104	11	rodríguez	rodríguez	NOUN
cvj-49	104	12	martínez	martínez	PROPN
cvj-49	104	13	,	,	PUNCT
cvj-49	104	14	h.	h.	PROPN
cvj-49	104	15	subtle	subtle	ADJ
cvj-49	104	16	membrane	membrane	NOUN
cvj-49	104	17	changes	change	NOUN
cvj-49	104	18	in	in	ADP
cvj-49	104	19	cryopreserved	cryopreserve	VERB
cvj-49	104	20	bull	bull	NOUN
cvj-49	104	21	semen	semen	NOUN
cvj-49	104	22	in	in	ADP
cvj-49	104	23	relation	relation	NOUN
cvj-49	104	24	with	with	ADP
cvj-49	104	25	sperm	sperm	NOUN
cvj-49	104	26	viability	viability	NOUN
cvj-49	104	27	,	,	PUNCT
cvj-49	104	28	chromatin	chromatin	NOUN
cvj-49	104	29	structure	structure	NOUN
cvj-49	104	30	and	and	CCONJ
cvj-49	104	31	field	field	NOUN
cvj-49	104	32	fertility	fertility	NOUN
cvj-49	104	33	.	.	PUNCT
cvj-49	105	1	theriogenology	theriogenology	NOUN
cvj-49	105	2	2003	2003	NUM
cvj-49	105	3	,	,	PUNCT
cvj-49	105	4	60	60	NUM
cvj-49	105	5	,	,	PUNCT
cvj-49	105	6	743	743	NUM
cvj-49	105	7	-	-	SYM
cvj-49	105	8	758	758	NUM
cvj-49	105	9	.	.	PUNCT
cvj-49	105	10	4	4	NUM
cvj-49	105	11	.	.	X
cvj-49	105	12	spinaci	spinaci	NOUN
cvj-49	105	13	,	,	PUNCT
cvj-49	105	14	m.	m.	NOUN
cvj-49	105	15	;	;	PUNCT
cvj-49	105	16	de	de	X
cvj-49	105	17	ambrogi	ambrogi	NOUN
cvj-49	105	18	,	,	PUNCT
cvj-49	105	19	m.	m.	NOUN
cvj-49	105	20	;	;	PUNCT
cvj-49	105	21	volpe	volpe	PROPN
cvj-49	105	22	,	,	PUNCT
cvj-49	105	23	s.	s.	PROPN
cvj-49	105	24	;	;	PUNCT
cvj-49	105	25	galeati	galeati	PROPN
cvj-49	105	26	,	,	PUNCT
cvj-49	105	27	g.	g.	PROPN
cvj-49	105	28	;	;	PUNCT
cvj-49	105	29	tamanini	tamanini	NOUN
cvj-49	105	30	,	,	PUNCT
cvj-49	105	31	c.	c.	PROPN
cvj-49	105	32	;	;	PUNCT
cvj-49	105	33	seren	seren	PROPN
cvj-49	105	34	,	,	PUNCT
cvj-49	105	35	e.	e.	PROPN
cvj-49	105	36	effect	effect	NOUN
cvj-49	105	37	of	of	ADP
cvj-49	105	38	staining	stain	VERB
cvj-49	105	39	and	and	CCONJ
cvj-49	105	40	sorting	sort	VERB
cvj-49	105	41	on	on	ADP
cvj-49	105	42	boar	boar	NOUN
cvj-49	105	43	sperm	sperm	NOUN
cvj-49	105	44	membrane	membrane	NOUN
cvj-49	105	45	integrity	integrity	NOUN
cvj-49	105	46	,	,	PUNCT
cvj-49	105	47	mitochondrial	mitochondrial	ADJ
cvj-49	105	48	activity	activity	NOUN
cvj-49	105	49	and	and	CCONJ
cvj-49	105	50	in	in	ADP
cvj-49	105	51	vitro	vitro	X
cvj-49	105	52	blastocyst	blastocyst	NOUN
cvj-49	105	53	development	development	NOUN
cvj-49	105	54	.	.	PUNCT
cvj-49	106	1	theriogenology	theriogenology	NOUN
cvj-49	106	2	2005	2005	NUM
cvj-49	106	3	,	,	PUNCT
cvj-49	106	4	64	64	NUM
cvj-49	106	5	,	,	PUNCT
cvj-49	106	6	191	191	NUM
cvj-49	106	7	-	-	SYM
cvj-49	106	8	201	201	NUM
cvj-49	106	9	.	.	PUNCT
cvj-49	106	10	5	5	NUM
cvj-49	106	11	.	.	X
cvj-49	106	12	nagy	nagy	NOUN
cvj-49	106	13	,	,	PUNCT
cvj-49	106	14	s.	s.	PROPN
cvj-49	106	15	;	;	PUNCT
cvj-49	106	16	jansen	jansen	PROPN
cvj-49	106	17	,	,	PUNCT
cvj-49	106	18	j.	j.	PROPN
cvj-49	106	19	;	;	PUNCT
cvj-49	106	20	topper	topper	NOUN
cvj-49	106	21	,	,	PUNCT
cvj-49	106	22	e.k	e.k	PROPN
cvj-49	106	23	.	.	PROPN
cvj-49	106	24	;	;	PUNCT
cvj-49	106	25	gadella	gadella	PROPN
cvj-49	106	26	,	,	PUNCT
cvj-49	106	27	b.m	b.m	PROPN
cvj-49	106	28	.	.	PROPN
cvj-49	106	29	a	a	DET
cvj-49	106	30	triple	triple	ADJ
cvj-49	106	31	stain	stain	NOUN
cvj-49	106	32	flow	flow	NOUN
cvj-49	106	33	cytometric	cytometric	ADJ
cvj-49	106	34	method	method	NOUN
cvj-49	106	35	to	to	PART
cvj-49	106	36	assess	assess	VERB
cvj-49	106	37	plasma	plasma	NOUN
cvj-49	106	38	and	and	CCONJ
cvj-49	106	39	acrosome	acrosome	VERB
cvj-49	106	40	membrane	membrane	NOUN
cvj-49	106	41	integrity	integrity	NOUN
cvj-49	106	42	of	of	ADP
cvj-49	106	43	cryopreserved	cryopreserve	VERB
cvj-49	106	44	bovine	bovine	NOUN
cvj-49	106	45	sperm	sperm	NOUN
cvj-49	106	46	immediately	immediately	ADV
cvj-49	106	47	after	after	ADP
cvj-49	106	48	thawing	thaw	VERB
cvj-49	106	49	in	in	ADP
cvj-49	106	50	presence	presence	NOUN
cvj-49	106	51	of	of	ADP
cvj-49	106	52	egg	egg	NOUN
cvj-49	106	53	-	-	PUNCT
cvj-49	106	54	yolk	yolk	NOUN
cvj-49	106	55	particles	particle	NOUN
cvj-49	106	56	.	.	PUNCT
cvj-49	107	1	biol	biol	PROPN
cvj-49	107	2	reprod	reprod	PROPN
cvj-49	107	3	2003	2003	NUM
cvj-49	107	4	,	,	PUNCT
cvj-49	107	5	68	68	NUM
cvj-49	107	6	,	,	PUNCT
cvj-49	107	7	1828	1828	NUM
cvj-49	107	8	-	-	SYM
cvj-49	107	9	1835	1835	NUM
cvj-49	107	10	.	.	PUNCT
cvj-49	108	1	6	6	X
cvj-49	108	2	.	.	X
cvj-49	108	3	garner	garner	NOUN
cvj-49	108	4	,	,	PUNCT
cvj-49	108	5	d.l	d.l	PROPN
cvj-49	108	6	.	.	PROPN
cvj-49	108	7	;	;	PUNCT
cvj-49	109	1	johnson	johnson	PROPN
cvj-49	109	2	,	,	PUNCT
cvj-49	109	3	l.a	l.a	PROPN
cvj-49	109	4	.	.	PROPN
cvj-49	109	5	viability	viability	NOUN
cvj-49	109	6	assessment	assessment	NOUN
cvj-49	109	7	of	of	ADP
cvj-49	109	8	mammalian	mammalian	ADJ
cvj-49	109	9	sperm	sperm	NOUN
cvj-49	109	10	using	use	VERB
cvj-49	109	11	sybr-14	sybr-14	PROPN
cvj-49	109	12	and	and	CCONJ
cvj-49	109	13	propidium	propidium	NOUN
cvj-49	109	14	iodine	iodine	NOUN
cvj-49	109	15	.	.	PUNCT
cvj-49	110	1	biol	biol	PROPN
cvj-49	110	2	reprod	reprod	PROPN
cvj-49	110	3	1995	1995	NUM
cvj-49	110	4	,	,	PUNCT
cvj-49	110	5	53	53	NUM
cvj-49	110	6	,	,	PUNCT
cvj-49	110	7	276	276	NUM
cvj-49	110	8	-	-	SYM
cvj-49	110	9	284	284	NUM
cvj-49	110	10	.	.	PUNCT
cvj-49	111	1	cluj	cluj	PROPN
cvj-49	111	2	vet	vet	PROPN
cvj-49	111	3	j	j	PROPN
cvj-49	111	4	2023	2023	NUM
cvj-49	111	5	,	,	PUNCT
cvj-49	111	6	vol	vol	NOUN
cvj-49	111	7	28	28	NUM
cvj-49	111	8	,	,	PUNCT
cvj-49	111	9	issue	issue	NOUN
cvj-49	111	10	48	48	NUM
cvj-49	111	11	7	7	NUM
cvj-49	111	12	.	.	PUNCT
cvj-49	112	1	peña	peña	PROPN
cvj-49	112	2	,	,	PUNCT
cvj-49	112	3	f.j	f.j	PROPN
cvj-49	112	4	.	.	PROPN
cvj-49	112	5	;	;	PUNCT
cvj-49	112	6	saravia	saravia	PROPN
cvj-49	112	7	,	,	PUNCT
cvj-49	112	8	f.	f.	PROPN
cvj-49	112	9	;	;	PUNCT
cvj-49	112	10	johannisson	johannisson	PROPN
cvj-49	112	11	,	,	PUNCT
cvj-49	112	12	a.	a.	NOUN
cvj-49	112	13	;	;	PUNCT
cvj-49	112	14	walgren	walgren	PROPN
cvj-49	112	15	,	,	PUNCT
cvj-49	112	16	m.	m.	NOUN
cvj-49	112	17	;	;	PUNCT
cvj-49	112	18	rodríguez	rodríguez	NOUN
cvj-49	112	19	-	-	PUNCT
cvj-49	112	20	martínez	martínez	PROPN
cvj-49	112	21	,	,	PUNCT
cvj-49	112	22	h.	h.	PROPN
cvj-49	112	23	a	a	DET
cvj-49	112	24	new	new	ADJ
cvj-49	112	25	and	and	CCONJ
cvj-49	112	26	simple	simple	ADJ
cvj-49	112	27	method	method	NOUN
cvj-49	112	28	to	to	PART
cvj-49	112	29	evaluate	evaluate	VERB
cvj-49	112	30	early	early	ADJ
cvj-49	112	31	membrane	membrane	NOUN
cvj-49	112	32	changes	change	NOUN
cvj-49	112	33	in	in	ADP
cvj-49	112	34	frozen	frozen	ADJ
cvj-49	112	35	-	-	PUNCT
cvj-49	112	36	thawed	thaw	VERB
cvj-49	112	37	boar	boar	NOUN
cvj-49	112	38	spermatozoa	spermatozoon	NOUN
cvj-49	112	39	.	.	PUNCT
cvj-49	113	1	int	int	PROPN
cvj-49	114	1	j	j	PROPN
cvj-49	114	2	androl	androl	PROPN
cvj-49	114	3	2005	2005	NUM
cvj-49	114	4	,	,	PUNCT
cvj-49	114	5	28(2	28(2	NUM
cvj-49	114	6	)	)	PUNCT
cvj-49	114	7	,	,	PUNCT
cvj-49	114	8	107	107	NUM
cvj-49	114	9	-	-	SYM
cvj-49	114	10	14	14	NUM
cvj-49	114	11	.	.	PUNCT
cvj-49	114	12	8	8	NUM
cvj-49	114	13	.	.	X
cvj-49	114	14	idziorek	idziorek	PROPN
cvj-49	114	15	,	,	PUNCT
cvj-49	114	16	t.	t.	PROPN
cvj-49	114	17	;	;	PUNCT
cvj-49	114	18	estaquier	estaquier	NOUN
cvj-49	114	19	,	,	PUNCT
cvj-49	114	20	j.	j.	PROPN
cvj-49	114	21	;	;	PUNCT
cvj-49	114	22	de	de	X
cvj-49	114	23	bels	bel	NOUN
cvj-49	114	24	,	,	PUNCT
cvj-49	114	25	f.	f.	PROPN
cvj-49	114	26	;	;	PUNCT
cvj-49	114	27	ameisen	ameisen	PROPN
cvj-49	114	28	,	,	PUNCT
cvj-49	114	29	j.c	j.c	PROPN
cvj-49	114	30	.	.	PROPN
cvj-49	114	31	yopro-1	yopro-1	PROPN
cvj-49	114	32	permits	permit	VERB
cvj-49	114	33	cytofluorometric	cytofluorometric	ADJ
cvj-49	114	34	analysis	analysis	NOUN
cvj-49	114	35	of	of	ADP
cvj-49	114	36	programmed	programmed	ADJ
cvj-49	114	37	cell	cell	NOUN
cvj-49	114	38	death	death	NOUN
cvj-49	114	39	(	(	PUNCT
cvj-49	114	40	apoptosis	apoptosis	NOUN
cvj-49	114	41	)	)	PUNCT
cvj-49	114	42	without	without	ADP
cvj-49	114	43	interfering	interfere	VERB
cvj-49	114	44	with	with	ADP
cvj-49	114	45	cell	cell	NOUN
cvj-49	114	46	viability	viability	NOUN
cvj-49	114	47	.	.	PUNCT
cvj-49	115	1	j	j	PROPN
cvj-49	115	2	immunol	immunol	NOUN
cvj-49	115	3	methods	method	NOUN
cvj-49	115	4	1995	1995	NUM
cvj-49	115	5	,	,	PUNCT
cvj-49	115	6	185	185	NUM
cvj-49	115	7	,	,	PUNCT
cvj-49	115	8	249	249	NUM
cvj-49	115	9	-	-	SYM
cvj-49	115	10	258	258	NUM
cvj-49	115	11	.	.	PUNCT
cvj-49	116	1	9	9	NUM
cvj-49	116	2	.	.	X
cvj-49	116	3	anzar	anzar	PROPN
cvj-49	116	4	,	,	PUNCT
cvj-49	116	5	m.	m.	NOUN
cvj-49	116	6	;	;	PUNCT
cvj-49	116	7	he	he	PRON
cvj-49	116	8	,	,	PUNCT
cvj-49	116	9	l.	l.	PROPN
cvj-49	116	10	;	;	PUNCT
cvj-49	116	11	buhr	buhr	PROPN
cvj-49	116	12	,	,	PUNCT
cvj-49	116	13	m.m	m.m	PROPN
cvj-49	116	14	.	.	PROPN
cvj-49	116	15	;	;	PUNCT
cvj-49	116	16	kroetsch	kroetsch	PROPN
cvj-49	116	17	,	,	PUNCT
cvj-49	116	18	t.g	t.g	PROPN
cvj-49	116	19	.	.	PROPN
cvj-49	116	20	;	;	PUNCT
cvj-49	117	1	pauls	pauls	PROPN
cvj-49	117	2	,	,	PUNCT
cvj-49	117	3	k.p	k.p	PROPN
cvj-49	117	4	.	.	PROPN
cvj-49	117	5	sperm	sperm	NOUN
cvj-49	117	6	apoptosis	apoptosis	NOUN
cvj-49	117	7	in	in	ADP
cvj-49	117	8	fresh	fresh	ADJ
cvj-49	117	9	and	and	CCONJ
cvj-49	117	10	cryopreserved	cryopreserve	VERB
cvj-49	117	11	bull	bull	NOUN
cvj-49	117	12	semen	seman	NOUN
cvj-49	117	13	detected	detect	VERB
cvj-49	117	14	by	by	ADP
cvj-49	117	15	flow	flow	NOUN
cvj-49	117	16	cytometry	cytometry	NOUN
cvj-49	117	17	and	and	CCONJ
cvj-49	117	18	its	its	PRON
cvj-49	117	19	relationship	relationship	NOUN
cvj-49	117	20	with	with	ADP
cvj-49	117	21	fertility	fertility	NOUN
cvj-49	117	22	.	.	PUNCT
cvj-49	118	1	biol	biol	PROPN
cvj-49	118	2	reprod	reprod	PROPN
cvj-49	118	3	2002	2002	NUM
cvj-49	118	4	,	,	PUNCT
cvj-49	118	5	66	66	NUM
cvj-49	118	6	,	,	PUNCT
cvj-49	118	7	354	354	NUM
cvj-49	118	8	-	-	SYM
cvj-49	118	9	360	360	NUM
cvj-49	118	10	.	.	PUNCT
cvj-49	118	11	10	10	NUM
cvj-49	118	12	.	.	PUNCT
cvj-49	118	13	kurz	kurz	PROPN
cvj-49	118	14	,	,	PUNCT
cvj-49	118	15	a.	a.	NOUN
cvj-49	118	16	;	;	PUNCT
cvj-49	118	17	viertel	viertel	PROPN
cvj-49	118	18	,	,	PUNCT
cvj-49	118	19	d.	d.	PROPN
cvj-49	118	20	;	;	PUNCT
cvj-49	118	21	herrmann	herrmann	PROPN
cvj-49	118	22	,	,	PUNCT
cvj-49	118	23	a.	a.	PROPN
cvj-49	118	24	;	;	PUNCT
cvj-49	118	25	müller	müller	PROPN
cvj-49	118	26	,	,	PUNCT
cvj-49	118	27	k.	k.	PROPN
cvj-49	118	28	localization	localization	PROPN
cvj-49	118	29	of	of	ADP
cvj-49	118	30	phosphatidylserine	phosphatidylserine	NOUN
cvj-49	118	31	in	in	ADP
cvj-49	118	32	boar	boar	NOUN
cvj-49	118	33	sperm	sperm	NOUN
cvj-49	118	34	cell	cell	NOUN
cvj-49	118	35	membranes	membrane	NOUN
cvj-49	118	36	during	during	ADP
cvj-49	118	37	capacitation	capacitation	NOUN
cvj-49	118	38	and	and	CCONJ
cvj-49	118	39	acrosome	acrosome	NOUN
cvj-49	118	40	reaction	reaction	NOUN
cvj-49	118	41	.	.	PUNCT
cvj-49	119	1	reproduction	reproduction	NOUN
cvj-49	119	2	2005	2005	NUM
cvj-49	119	3	,	,	PUNCT
cvj-49	119	4	130	130	NUM
cvj-49	119	5	,	,	PUNCT
cvj-49	119	6	615	615	NUM
cvj-49	119	7	-	-	SYM
cvj-49	119	8	626	626	NUM
cvj-49	119	9	.	.	PUNCT
cvj-49	119	10	11	11	NUM
cvj-49	119	11	.	.	PUNCT
cvj-49	120	1	martínez	martínez	PROPN
cvj-49	120	2	-	-	PUNCT
cvj-49	120	3	pastor	pastor	PROPN
cvj-49	120	4	,	,	PUNCT
cvj-49	120	5	f.	f.	PROPN
cvj-49	120	6	;	;	PUNCT
cvj-49	120	7	mata	mata	PROPN
cvj-49	120	8	-	-	PUNCT
cvj-49	120	9	campuzano	campuzano	NOUN
cvj-49	120	10	,	,	PUNCT
cvj-49	120	11	m.	m.	NOUN
cvj-49	120	12	;	;	PUNCT
cvj-49	120	13	álvarez	álvarez	NOUN
cvj-49	120	14	-	-	PUNCT
cvj-49	120	15	rodríguez	rodríguez	NOUN
cvj-49	120	16	,	,	PUNCT
cvj-49	120	17	m.	m.	NOUN
cvj-49	120	18	;	;	PUNCT
cvj-49	120	19	álvarez	álvarez	NOUN
cvj-49	120	20	,	,	PUNCT
cvj-49	120	21	m.	m.	NOUN
cvj-49	120	22	;	;	PUNCT
cvj-49	120	23	anel	anel	PROPN
cvj-49	120	24	,	,	PUNCT
cvj-49	120	25	l.	l.	PROPN
cvj-49	120	26	;	;	PUNCT
cvj-49	120	27	de	de	PROPN
cvj-49	120	28	paz	paz	PROPN
cvj-49	120	29	,	,	PUNCT
cvj-49	120	30	p.	p.	NOUN
cvj-49	120	31	probes	probe	NOUN
cvj-49	120	32	and	and	CCONJ
cvj-49	120	33	techniques	technique	NOUN
cvj-49	120	34	for	for	ADP
cvj-49	120	35	sperm	sperm	NOUN
cvj-49	120	36	evaluation	evaluation	NOUN
cvj-49	120	37	by	by	ADP
cvj-49	120	38	flow	flow	NOUN
cvj-49	120	39	cytometry	cytometry	NOUN
cvj-49	120	40	.	.	PUNCT
cvj-49	121	1	reprod	reprod	PROPN
cvj-49	121	2	dom	dom	PROPN
cvj-49	121	3	anim	anim	PROPN
cvj-49	121	4	2010	2010	NUM
cvj-49	121	5	,	,	PUNCT
cvj-49	121	6	45	45	NUM
cvj-49	121	7	,	,	PUNCT
cvj-49	121	8	67	67	NUM
cvj-49	121	9	-	-	SYM
cvj-49	121	10	78	78	NUM
cvj-49	121	11	.	.	PUNCT
cvj-49	122	1	1	1	NUM
cvj-49	122	2	.	.	X
cvj-49	122	3	introduction	introduction	NOUN
cvj-49	122	4	2	2	NUM
cvj-49	122	5	.	.	PUNCT
cvj-49	122	6	semen	semen	NOUN
cvj-49	122	7	sample	sample	NOUN
cvj-49	122	8	preparation	preparation	NOUN
cvj-49	122	9	3	3	X
cvj-49	122	10	.	.	PUNCT
cvj-49	122	11	instrument	instrument	NOUN
cvj-49	122	12	setup	setup	NOUN
cvj-49	122	13	4	4	NUM
cvj-49	122	14	.	.	PUNCT
cvj-49	122	15	sperm	sperm	NOUN
cvj-49	122	16	viability	viability	NOUN
cvj-49	122	17	assessment	assessment	NOUN
cvj-49	122	18	5	5	NUM
cvj-49	122	19	.	.	PUNCT
cvj-49	122	20	evaluation	evaluation	NOUN
cvj-49	122	21	of	of	ADP
cvj-49	122	22	acrosome	acrosome	NOUN
cvj-49	122	23	reaction	reaction	NOUN
cvj-49	122	24	6	6	NUM
cvj-49	122	25	.	.	PUNCT
cvj-49	123	1	evaluation	evaluation	NOUN
cvj-49	123	2	of	of	ADP
cvj-49	123	3	mitochondrial	mitochondrial	ADJ
cvj-49	123	4	activity	activity	NOUN
cvj-49	123	5	7	7	NUM
cvj-49	123	6	.	.	PUNCT
cvj-49	124	1	conclusions	conclusion	NOUN
cvj-49	124	2	references	reference	NOUN