id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
cusj-10965	Lang, Elvis; Tilton, John; Sweet, Thomas	Expression of Single mRNA Constructs Encoding Both CRISPR-Cas9 Protein and Guide RNAs for Future Gene Therapy Applications: 2023 Article of The Year	2024	11	.pdf	application/pdf	4725	242	55	On the other hand, non-viral meth- ods utilize other macromolecules like lipid nanoparticles (LNPs), protein polymers, and Au nanoparticles to deliver gRNA and Cas9 mRNA, protein, or DNA.10 Delivering the pro- tein form of CRISPR-Cas9 along with gRNA is efficient and doesn’t result in much off-tar- get editing, however it is very expensive and there is risk of endotoxin contamination.10 Cas9 mRNA + gRNA delivery has similar Figure 2: Sequence of PCTG plasmids involved with Cas9 gene editing. al. 9 benefits to the protein form; however, it re- quires two components to get into particles and cells.10 Alternatively, Cas9 delivery can also be performed through complexing and conju- gating with proteins.	cache/cusj-10965.pdf	txt/cusj-10965.txt