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ARTICLEVOLUME	2,	SPRING	2007	

Differentiation	of	Two	Species	of	MUllerian	Inhibitory	
Substance	(MIS)	

 

Andrew	R	Bader1,		

Douglas	Lim2,		

David	MacLaughlin3	

1	Columbia	College,	Columbia	University,	

2	Clarkson	University,	

3	Pediatric	Research	Laboratory,	Massachusetts	General	Hospital	

Abstract	
Muellerian	inhibiting	substance	(MIS),	also	known	as	Anti-Muellerian	
hormone,	is	a	testicular	protein	responsible	for	the	regression	of	the	
MUllerian	duct.	In	vivo	and	in	vitro	experiments	using	MIS	have	shown	it	
to	inhibit	the	growth	of	various	tumors,	including	certain	ovarian	
cancers.	Two	approaches	to	purifying	the	product	have	been	used,	
immunoaffinity	purification	and	chromatography.	The	purified	products	
were	tested	for	affinity	to	binding	sites	using	the	enzyme	linked	
immunoabsorbent	assay	(ELISA).	The	goal	of	this	study	was	to	test	the	
theory	that	the	two	MIS	proteins	identified,	termed	IAP	and	Q,	were	
different	on	structural	and	molecular	levels.	The	results	of	these	
investigations	reveal	the	existence	of	two	distinct	types	of	MIS	
differentiated	by	ELISA	which	based	on	future	study	may	have	different	
impacts	as	cancer	treatment	agents.