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© 2021 Conscientia Beam. All Rights Reserved. 

AZOTOBACTER AND TOTAL BACTERIAL POPULATION IN ENRICHED COMPOST: 
PRELIMINARY STUDY   

 

 

 Reginawanti  
Hindersah1+ 

 Agustinus Marthin  
Kalay2 

1Faculty of Agriculture, Universitas Padjadjaran Jalan Raya Bandung-
Sumedang, Indonesia. 

 
2Faculty of Agriculture, Universitas Pattimura, Jalan Ir. Putuhena, Poka 
Campus, Indonesia.  

 
 
 

 
(+ Corresponding author) 

 ABSTRACT 
 
Article History 
Received: 14 January 2021 
Revised: 11 February 2021 
Accepted: 5 March 2021 
Published: 25 March 2021 
 

Keywords 
Azotobacter 
Total bacteria 
Sterilized compost 
Compost quality 
Microbial density 
Compost acidity  
Compost humidity. 

 
Compost enrichment with nitrogen fixing Azotobacter is a method to enhance the used 
of biofertilizer in sustainable agriculture. The objective of a laboratory experiment was 
to verify the change in Azotobacter and total bacteria population in two kind of 
compost after inoculation with nitrogen fixing A. chroococcum. The experiment was 
setup in completely randomized design with four combination treatments of sterilized 
and unsterilized composted manure as well as vermicompost. The control treatments 
are the sterilized one. The results verified that irrespective of treatments, Azotobacter 
enrichment increased Azotobacter but reduced total bacteria population after 18-day 
incubation compared to the composts before treatment. At the end of experiment, the 
population of Azotobacter in both sterilized and unsterilized vermicompost were 
slightly lower than in manure; but the count total heterotroph bacteria in both organic 
matter was similar. The acidity of both enriched compost was neutral while their 
humidity was declined to 18-19% at the end of experiment. This experiment showed 
that inoculating the compost with Azotobater maintain the Azotobacter count but 
decline the total bacteria. This suggested that negative interaction between 
Azotobacter and other heterotroph bacteria in compost might be occured.  
 

Contribution/Originality: This study is one of very few studies about the viability of total bacteria and 

Azotobacter in Azotobacter-enriched compost. Compost enrichment with Azotobacter enhanced Azotobacter 

population but decreased total bacteria decreased in the compost. This enriched compost can serve as organic 

matter and Azotobacter inoculant once introducing to the soil. 

 

1. INTRODUCTION 

Organic fertilizer as well as biofertilizer is suggested as natural input to optimize plant growth and yield crops 

in sustainable agriculture. Intensive agriculture in general introduce high rates of inorganic fertilizer which cause a 

detrimental effect on soil.  Organic matter amendment has been proven to maintain soil health since organic carbon 

(C) in soil support the dynamic living system that in turn support microbial diversity and nutrient cycle [1]. 

Maintaining organic C stock in arable soils is importance for nutrient supplying system which accommodate by 

microbial activity [2]. Nowadays, the application on biofertilizer is not as intensive as organic fertilizer. In 

Indonesia, the constraint of using biofertilizer is - among others - time consuming. The growers need to allocate 

significant time to inoculate the plants during crops cultivation. Usually, the growers mix organic matter with soil 

Current Research in Agricultural Sciences 
2021 Vol. 8, No. 1, pp. 31-36. 
ISSN(e): 2312-6418 
ISSN(p): 2313-3716 
DOI: 10.18488/journal.68.2021.81.31.36 
© 2021 Conscientia Beam. All Rights Reserved. 

 
 
 

 
 
 

 

 
 
 
 

https://orcid.org/0000-0003-0281-2363
https://orcid.org/0000-0002-0875-040X
https://www.doi.org/10.18488/journal.68.2021.81.31.36


Current Research in Agricultural Sciences, 2021, 8(1): 31-36 

 

 
32 

© 2021 Conscientia Beam. All Rights Reserved. 

during the soil preparation and dress chemical (and bio-) fertilizer when the crops are already grown. In order to 

overcome this constraints, enriching compost with biofetilizer is an efficient way to introduce the biofertilizer at the 

same time of organic matter amendment. Composting is an appropriate and accepted system elsewhere to stabilize 

organic matter [3] so that composts are used elsewhere in Indonesia.Non-symbiotic nitrogen (N) fixing 

Azotobacter now is broadly used as active ingredients in biofertilizer industry. This plant growth promoting 

rhizobacteria induce plant growth not only by N fixation but also by phytohormones and exopolysaccharides 

production [4-6].  Enriching compost before introducing to soil with Azotobacter inoculant become a very simple 

method to introduce N fixing bacteria. In tropics, the soil usually contains low amount of N because of high 

precipitation and daily temperature [7] and farmers anticipate the low avilability N during plant growth by 

introducing significant amount of N fertilizer, mainly urea. By using N fixing biofertilizer, the level of N fertilizer 

will be reduced and hence protect the soil health. Compost enrichment with microbial inoculant has a substantial 

effect to increase the consumption of biofertilizer. Enrichment of compost has been shown to enhance the microbial 

count and nutrient content of compost [8]. The count of beneficial microbes includes Azotobacter increased 

significantly in the compost during 30-day incubation although the microbial enrichment but didn’t affect the level 

of major macronutrients N, P as well as K [9]. The phytohormones content was higher in the microbial-enriched 

compost [10]. Change in microbial population is reported following microbial enrichment on compost. The total 

count of N-fixing Azospirillum brasilense and Rhizobium leguminosarum in enriched vermicompost was high during 

the early phases of incubation but the population was decreased at 180-day storage [11]. Enrichment compost of 

mushroom logs with Azotobacter-Pseudomonas consortia results in the enhancement of Azotobacter population in 

compost at 21-day storage up to 106 CFU/g and then the population reduction is recorded at 7 days later [12]. 

The quality of compost include chemical and biological traits is depending largely on the organic material 

composition before composting. Moreover, the nutrient composition of similar plant-origin organic matter is 

determined by the soil where they grow as well as nutrient management during growing period. The microbial 

enrichment of composted organic matter will result in the different count of target microbes in the compost. The 

objective of this preliminary study was to verify the population of Azotobacter and total bacteria, as well as the 

acidity and humidity of two kind of compost after Azotobacter chroococcum enrichment.  

 

2. MATERIAL AND METHODS 

2.1. Azotobacter Inoculant and Compost 

The Azotobacter chroococcum liquid inoculant was provided by Soil Biology Laboratory, Faculty of Agriculture 

Universitas Padjadjaran, Indonesia, located in tropical area at the altitude of 735 m above the sea level.  The liquid 

inoculant was produced by using molasses-based broth enriched with low concentration of N.  During 30-day 

storage, the population of Azotobacter in the liquid inoculant was 107 CFU/mL. This experiment enriched two kind 

of compost. The cow manure-based compost from Universitas Padjadjaran and the vermicompost was produced by 

sugar factory in Jatitujuh which belong to PT Rajawali Nusantara. The acidity of compost was 6,8 with organic C 

10,85%, N 0,57%, P2O5 0,65% and K2O 0,88%. The vermicompost has the pH of 7.1 and contained 1.12% N, 2.31 % 

P2O5 and 1.04 %, K2O. The humidity of compost and cast before experiment was 29%. 

 

2.2. Experimental Setup 

The design of laboratory trial was in completely randomized design with four replications and four treatments 

that consisted of sterilized and unsterilized composted manure and vermicompost. The control treatments are the 

sterilized one. Sterilization method was wet sterilization by using autoclave at 120oC for 20 minutes. A total of 100 

g compost and vermicompost were placed in transparent polyethylene bag and autoclaving before Azotobacter 

enrichment in the next day. Before introducing liquid inoculant on the 100 g of compost, 10 mL liquid inoculant of 

Azotobacter (cell density of 107 CFU/mL) was diluted with 10 mL of sterilized 0,85% sodium chloride and mixed 



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© 2021 Conscientia Beam. All Rights Reserved. 

evenly in sterilized test tube.  The compost was removed from polyethylene bag and placed in clean black 

polyethylene bag.  Diluted liquid inoculant was pouring evenly on the surface of compost and mixed evenly by 

using sterilized stick. After homogenization, the bags were covered with perforated thick white paper to avoid high 

evaporation but maintain air circulation. This enrichment method resulted in the population of Azotobacter up to 

about 106 CFU/mL in compost.  The humidity of both compost after enrichment was 29%.  Enriched compost then 

stored at room temperature (15-27oC) for 18 days without direct sun light.   

 

2.3. Parameters and Statistical Analysis 

Azotobacter and total bacteria population has been counted once in three days during 18-days storage. The 

first count has been carried out at inoculation day; precisely about one hours after inoculation. The methods of 

population counting was serial dilution plate method [13] in N-free Asbhy plate for Azotobacter and Nutrient Agar 

plate for total aerobic-heterotroph bacteria. The acidity and humidity of enriched compost and vermicompost were 

measured at the end of experiment by AOAC Methods [14]. All data were subjected to analysis of variance with 

p<0.05. If the sum square of the treatment was significant then the Least Significance Difference (LSD) tests with 

p<0.05 were performed. The Statistical analysis used Minitab 18 program.  

 

3. RESULTS AND DISCUSSION 

3.1. Bacterial Population 

To verify the viability of Azotobacter and total bacteria in compost without interaction with indigenous 

microbes, sterilized compost and vermicompost were the control treatments. Two hours after enrichment, the 

population of Azotobacter in both sterilized and unsterilized compost was about 6 log10 CFU/g (Figure 1a).  

The Azotobacter count increased during 6 days and declined from 6-18 days. However, after 18-days storage 

the highest population of Azotobacter demonstrated by sterilized compost either compost or vermicompost (Figure 

1a). The growth peak of Azotobacter population in compost was recorded at 6-day incubation but highest 

Azotobacter count in sterilized compost was at day 9.  At the end of experiment, population of Azotobacter in both 

enriched sterilized compost was 1 log higher than before inoculation compared to unsterilized one. 

 

a b 
Figure-1. The population of Azotobacter (a) and total bacteria (b) in sterilized and unsterilized organic matter during 18 days Azotobacter 
inoculation. SCM: sterilized composted manure, USM: unsterilized composted manure; SWC: sterilized vermicompost; UWC: unsterilized 
vermicompost. 
Source: Primary data of the research. 

 

Total bacteria counted by nutrient verified that bacterial population was ecreased sharply after 18-day 

incubation (Figure 1b). The bacteria grown in plate agar were aerobic and heterotrophic bacteria since the isolated 

media was nutrient agar that contain organic C. At the day of inoculation, the population of total bacteria reached 6 



Current Research in Agricultural Sciences, 2021, 8(1): 31-36 

 

 
34 

© 2021 Conscientia Beam. All Rights Reserved. 

log10 of CFU/mL and 7 log10 CFU/mL in sterilized and unsterilized compost which is almost equal to the 

population before inoculation The population increased during 6-day incubation and declined from day 9 to day 18. 

Based on Duncan test, at day 6, Azotobacter population in sterilized vermicompost was significantly higher 

than in sterilized compost Table 1. However, at day 18 the its density in unsterilized compost were 1 log higher 

than sterilized one.  

 
Table-1. The difference between Azotobacter and total bacteria population in sterilized and unsterilized compost 
after at 6, 12 and 18-day enrichment with Azotobacter. 

Treatments 
Log10 CFU/mL Azotobacter 

Day 0 Day 6 Day 12 Day 18 

Sterilized composted manure 6.88 b 6.86 b 7.22 a 7.02 a 
Unsterilized composted manure 6.68 c 7.72 a 6.72 b 6.54 b 
Sterilized vermicompost 6.81 b 8.01 a 7.10 a 7.04 a 
Unsterilized vermicompost 6.97 a 7.98 a 6.54 c 6.59 b 

Treatment 
Log10 total bacterial population 

Day 0 Day 6 Day 12 Day 18 
Sterilized composted manure 6.11 d 7.34 a 6.54 a 5.27 a 
Unsterilized composted manure 7.87 a 7.41 a 6.09 b 5.29 a 
Sterilized vermicompost 6.52 c 6.82 b 6.06 b 5.22 a 
Unsterilized vermicompost 7.73 b 7.36 a 6.61 a 5.26 a 

 

 

Numbers followed by the same letters are not significantly differ based on LSD test p<0.05. 

Lower bacterial population showed by sterilized vermicompost at 6-day incubation but 9 days later, all bacterial 

population in unsterilized compost were lower than sterilized one. At the end of experiment, total bacterial count 

decreased up to 5 log10 CFU/mL and there was no cell count difference between both sterilized and unsterilized 

compost. 

 

3.2. Acidity and Humidity of Compost 

Analysis of variance showed that the Azotobacter enrichment to different compost didn’t affect humidity but 

decreased the acidity compare to untreated compost. Nonetheless, based on LSD test (p<0.05), the acidity and 

humidity of sterilized/unsterilized compost and vermicompost was not differ at 18 day-incubation (Figures 2a and 

2b). Before inoculation, the acidity of compost and vermicompost were 6.8 and 7.1 respectively. Slightly increased in 

compost acidity was demonstrated at the end of incubation (Figure 2a). The humidity of both compost after 

Azotobacter liquid inoculant application was up to 29%; and at the end of incubation they reduced to less than 20% 

(Figure 2b). 

 

 

a b 
Figure-2. The acidity (a) and humidity (b) of manure compost and vermicompost 18 days after enrichment with Azotobacter liquid inoculant. 
SCM: sterilized composted manure, USM: unsterilized composted manure;      SWC: sterilized vermicompost; UWC: unsterilized  vermicompost 



Current Research in Agricultural Sciences, 2021, 8(1): 31-36 

 

 
35 

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Composted manure as well as vermicompost serve as a source of nutrients and physical protection to 

proliferation of heterotrophic bacterial. In heterotroph metabolisms, microbes utilized organic matter as carbon 

source and to generate energy [15].  Both compost support the growth of Azotobacter but less supportive to 

maintain bacterial population during 18-day incubation.  

Before the enrichment, the Azotobacter count in sterilized and unsterilized composted manure and 

vermicompost were less than 103 CFU/g (3 log10). The population of total bacteria in sterilized compost was 2 x105 

lower than unsterilized one; almost 3 x 106 CFU/mL. This research showed that the population of Azotobacter was 

increased from day 3 to day 18; but the enrichment of organic matte compost with Azotobacter cannot maintain 

total bacterial population The increased of Azotobacter agreed with the similar trial by using waste mushrooms log 

compost vermicompost and farmyard manure [9, 12]. Both researcher verified that compost enable to maintain 

Azotobacter population up to 21 days.  

Azotobacter is heterotroph rhizobacteria that forms cysts under dry condition [14]. At the end of  experiment, 

the humidity of  compost was declined from 29 % to up to 18%. The decrease of  humidity induces the cyste 

formation [16].  Moreover, the acidity (pH) of either sterilized and unsterilized organic matters were about neutral. 

The Azotobacter proliferate better in neutral pH and they are sensitive to acid environment [17]. The decrease of  

total bacteria which is enumerated on nutrient agar plate, might be the results of  the negative interaction with 

exogenous Azotobacter as well as abiotic stress condition. This antagonistic interaction due to abiotic stress hence 

influence the impact on plant performance [18]. The native bacteria in compost compete the nutrients and water 

with exogenous Azotobacter. Moreover, the low humidity distresses the metabolisms of  native microbes.  Both of  

condition resulted in significant reduced of  total bacteria in compost between 3 – 18 days after inoculation.  

Introducing beneficial microbes to both compost increased Azotobacter count. However microbial enrichment 

also will increase its quality but this research didn’t analyze the chemical properties of  compost. For further 

research. longer shelf  life should be evaluated to ensure the viability of  Azotobacter and the change of  physico-

chemical properties of  enriched compost.  

 

4. CONCLUSION 

Compost enrichment with Azotobacter liquid inoculant resulted in the increase of Azotobacter count and 

decreased in total bacteria population after 18-day incubation at room temperature. The acidity of enriched compost 

was about neutral but the humidity was decreased from 29% before enrichment to 18-19% at the end of experiment. 

In order to increase the used of biofertilizer, compost enrichment by using beneficial soil microbes is suggested. 

 

Funding: This research is funded by Laboratory of Soil Biology, Faculty of Agriculture, 
Universitas Padjadjaran, Indonesia. 
Competing Interests: The authors declare that they have no competing interests.  
Acknowledgement: Authors thank sugar factory of Jatitujuh in Majalengka, West Java to 
provide the vermicompost 

 

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