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© 2024 Conscientia Beam. All Rights Reserved. 

Enhancing Broccoli growth by bacillus rhizosphere bacteria   

 

 

 Nisrina Salsabila1+ 

 Reginawanti 
Hindersah2 

 Betty Natalie 
Fitriatin3 

 

1,2,3Department of Soil Science and Land Resources, Faculty of Agriculture, 
Universitas Padjadjaran, Sumedang, Indonesia. 
1Email: nisrina19008@mail.unpad.ac.id  
2Email: reginawanti@unpad.ac.id  
3Email: betty.natalie@unpad.ac.id   

(+ Corresponding author) 

 ABSTRACT 
 
Article History 
Received: 15 July 2024 
Revised: 26 September 2024 
Accepted: 7 October 2024 
Published: 21 October 2024 
 

Keywords 
Bacillus 
Broccoli 
Growth curves 
Nutrients 
Phytohormones 
Plant growth 
Biofertilizer. 

 
This study examines the enhancement of broccoli growth by Bacillus Rhizosphere. 
Bacteria Broccoli is a nutrient-rich vegetable known for its high content of polyphenols, 
flavonoids, vitamins, minerals, fiber, and low calorie count, making it a recommended 
daily food. Tropical regions grow broccoli in mountainous areas. Sustainable 
agriculture emphasizes minimizing chemical fertilizers, and biofertilizers like Plant 
Growth Promoting Rhizobacteria (PGPR), such as Bacillus, can reduce their use. 
Bacillus improves plant growth by fixing nitrogen, solubilizing phosphate and 
potassium, and producing phytohormones. Understanding Bacillus growth dynamics is 
vital for optimizing its agricultural application. This study aimed to analyze growth 
curves of different Bacillus species and investigate their potential role in promoting 
broccoli growth. This study was carried out in January–April 2024 at the Soil Biology 
Laboratory, Faculty of Agriculture, Universitas Padjadjaran, and Bumi Agro 
Technology Company, Lembang. The research was divided into 3 stages: growth curve 
determination, Bacillus liquid inoculant preparation, and application of treatment to 
plants. The findings showed that isolates of B. safensis strain MDL5, B. altitudinis strain 
RPW2, B. subtilis strain YPS4, and Bacillus sp. strain SZ057 reached the highest point 
in their growth curves after 4 and 5 days of incubation. After that, the curves slowly 
went down. Inoculating Bacillus into broccoli plants resulted in an increase in plant 
height by 43.28–58.75% compared to the control.  
 

Contribution/Originality: The study adds to what is known about using biofertilizers on broccoli in tropical 

mountainous areas. It shows that adding Bacillus leads to a significant increase in productivity, which in turn leads 

to plants that are 43.28–58.75% trailer. This underscores Bacillus' potential in reducing chemical fertilizer use and 

promoting sustainable agriculture. 

 

1. INTRODUCTION 

Broccoli is a nutrient-rich vegetable widely cultivated for its health benefits and economic value [1]. In the 

past decade, broccoli consumption has increased significantly due to awareness of its high content of polyphenols, 

flavonoids, and fiber, as well as its low calorie count. Broccoli contains high levels of vitamins, antioxidants, and 

anticarcinogenic compounds; therefore, all food authorities worldwide recommend its daily consumption [2]. 

Mountainous areas in tropical regions grow broccoli. Fertilization effectively achieves high broccoli production. In 

sustainable agriculture, it is crucial to minimize the use of chemical fertilizers. Several studies report that the use of 

biofertilizer can reduce the dose of chemical fertilizer [3-5]. Biofertilizer can be Plant Growth Promoting 

Current Research in Agricultural Sciences 
2024 Vol. 11, No. 2, pp. 56-63 
ISSN(e): 2312-6418 
ISSN(p): 2313-3716 
DOI: 10.18488/cras.v11i2.3945 
© 2024 Conscientia Beam. All Rights Reserved. 

 
 
 

 
 
 
 

 

 
 
 
 

https://orcid.org/0009-0009-6411-0018
https://orcid.org/0000-0003-0281-2363
https://orcid.org/0000-0003-3632-4317
mailto:nisrina19008@mail.unpad.ac.id
mailto:reginawanti@unpad.ac.id
mailto:betty.natalie@unpad.ac.id
https://www.doi.org/10.18488/cras.v11i2.3945


Current Research in Agricultural Sciences, 2024, 11(2): 56-63 

 

 
57 

© 2024 Conscientia Beam. All Rights Reserved. 

Rhizobacteria (PGPR) inoculant, which can increase plant growth. ne of the prominent PGPRs (Plant Growth 

Promoting Rhizobacteria) in agriculture is Bacillus. Bacillus lives in various plant rhizospheres, including food crops. 

The genus Bacillus, comprising a diverse group of Gram-positive, rod-shaped bacteria, has received significant 

attention in microbiological and agricultural research [6]. Bacillus as PGPR can boost plant growth through 

various mechanisms: nitrogen fixation, phosphate solubilization, potassium solubilization, phytohormone 

production, and as biocontrol agent [7]. Bacillus produces nitrogenase, which catalyzes the conversion of molecular 

dinitrogen (N2) to ammonia (NH3), allowing plant roots to absorb it. Additionally, Bacillus excretes five organic 

acids, namely acetic, gluconic, succinic, lactic, and propionic acids, that helps solubilize phosphate [8]. Bacillus 

species are renowned for their ability to form endospores. Bacillus form spores when conditions are unfavorable for 

growth. The spores are resistant to heat, cold, radiation, desiccation, and disinfectants [9]. A sporulating bacterium 

develops these spores inside its mother cell, which breaks apart and releases them into the environment. These 

spores are highly resistant to various environmental stressors and serve as a survival mechanism [10]. 

Bacillus is able to produce phytohormones such as auxin, cytokinin, gibberellin, and abscisic acid [11]. Auxin is 

a hormone that triggers tissue differentiation, cell elongation, and cell division in plants [12]. Gibberellin plays a 

role in root and shoot elongation, seed germination, flowering, and fruit pattern [13]. Cytokinins play an important 

role in physiological processes [14]. Abscisic acid plays a role in stomata closure, fruit delivery, and seed 

germination [15]. Understanding the characteristics and growth dynamics of Bacillus is crucial for optimizing their 

application in agricultural practices. Growth curve analysis helps in determining the optimal conditions for 

bacterial growth that are essential for maximizing their beneficial effects on plants. Biofertilizer inoculant 

production requires knowledge of the bacterial growth phases. Mostly bacterial inoculants are collected from the 

exponential phase, where the bacteria grows rapidly. In Indonesia, broccoli is high-economic-value vegetable crop 

that is consistently grown using chemical fertilizers. There has been limited research on the use of biofertilizers in 

high-economic-value vegetable crops in Indonesia. Thus, this study focuses on analyzing the growth curves of 

different Bacillus species and evaluating their essential role in promoting broccoli growth in a pot experiment. 

 

2. MATERIALS AND METHODS 

The experiment was carried out in January–April 2024 at the Soil Biology Laboratory, Faculty of Agriculture, 

Padjadjaran University, and CV. Bumi Agro Technology, Lembang District, West Bandung, West Java. The 

altitude of this tropical mountainous area was 1.200 m above sea level, and the average temperature during the 

experiment was 17–29 °C. 

The bacterial isolates used in this experiment consisted of Bacillus safensis strain MDL5, Bacillus altitudinis 

strain RPW2, Bacillus subtilis strain YPS4, and Bacillus sp. strain SZ057. The Soil Biology Laboratory at 

Padjadjaran University provided the four bacterial isloates. Figure 1 shows images of the Bacillus isolates used in 

the experiment. 

 

    



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© 2024 Conscientia Beam. All Rights Reserved. 

    
Bacillus safensis strain 

MDL5 
Bacillus altitudinis 

strain RPW2 
Bacillus subtilis strain 

YPS4 
Bacillus sp. strain 

SZ057 
Figure 1. Bacillus isolates used in the experiment. 

 

2.1. Growth Curve Determination 

This stage begins with refreshing the bacterial isolate onto Tryptic Soy Agar (TSA) slant agar media, 

incubating at 30 °C for 48 hours. To make a liquid culture, inoculate 0.5% starter into new Tryptic Soy Broth (TSB) 

media and incubate on a shaker. Bacterial liquid culture measured its population, Optical Density (OD), and pH for 

7 days, then a curve was made. Bacterial population in TSB media was counted using serial dilution plate method. 

 

2.2. Bacillus Liquid Inoculant Preparation 

To make the liquid inoculant for plants, prepare 600 mL of new TSB media for each isolate. Then 0.5% liquid 

culture was inoculated into new TSB medium and incubated on a shaker at 180 rpm for 72 hours.  

 

2.3. Experimental Design 

The experimental design was Randomized Block Design to test four types of Bacillus inoculant and one control 

treatment, including: 

A = Control (without Bacillus inoculation). 

B = Bacillus safensis strain MDL5. 

C = Bacillus altitudinis strain RPW2. 

D = Bacillus subtilis YPS4. 

E = Bacillus sp. strain SZ057. 

F = Konsorsium Bacillus. 

Each treatment was replicated 7 times. In control plants, a 100% dose of Nitrogen (N), Phosphate (P), dan 

Potassium (K) fertilizer was applied, while in plants treated with Bacillus incubation, 75% N, 75% P, and 100% K 

were applied. 

 

2.4. Experimental Setup 

The broccoli plants were grown in pots as shown in Figure 2. This experiment used planting media in the form 

of a mixture of soil and manure in a ratio of 2:1. Planting was carried out by transplanting 4 week-old broccoli 

seedlings. Bacterial inoculation treatment is given through soil application by spraying 25 mL of bacterial 

suspension onto the soil three days before planting and a week after planting, respectively. Broccoli was planted for 

up to 3 weeks. Plant height and number of leaves were measured once a week. Meanwhile, stem diameter was 

measured 3 weeks after planting. All plant growth data were analyzed using analysis of variance (p<0.05). If the 

treatments had a significant effect on the parameters, the Duncan Multiple Range Test was conducted. IBM SPSS 

Statistic software performed the analysis.  

 



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© 2024 Conscientia Beam. All Rights Reserved. 

 
Figure 2. 3 weeks old broccoli in pot. 

 

3. RESULTS 

The four Bacillus isolates on TSB media were measured for growth curves, optical density at 600 nm, and media 

pH for 6 days. T indicates the bacterial culture’s incubation time. 

 

 
Figure 3. Bacillus growth curve at 0 – 6 days after incubation. 

 

Figure 3 shows that the growth curves of the four Bacillus bacterial isolates have almost the same curve 

pattern. The four bacterial isolates in the lag phase had a population of 105. Isolates of B. safensis MDL5 and 

Bacillus sp. SZ057 reached peak growth at T4, while B. altitudinis RPW2 and B. subtilis YPS4 reached peak growth 

at T5. 

 

 
Figure 4. Optical density of bacillus growth curve at 0 – 6 days after incubation. 



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Figure 4 shows the optical density (OD) measurement of the bacterial growth curve. The OD value on the 

bacterial growth curve tends to increase over time. The curve experienced a drastic increase at T1 for the four 

bacterial isolates, then rose slowly thereafter. 

 

 
Figure 5. Bacillus pH curve at 0 – 6 days after incubation. 

 

Figure 5 depicts bacterial growth’s pH curve. At T0, the four bacterial isolates showed a neutral pH, caused by 

the pH of the TSB medium, which was also neutral (7.08). At T1, the pH decreased in four bacterial isolates. Then, 

at T2 onwards, the pH of the four bacterial isolates increased slowly. 

 

Table 1. Height of broccoli plant grown with bacillus inoculation at 1 – 3 weeks after planting (WAP). 

Treatment 
Plant height (cm) 

1 WAP 2 WAP 3 WAP 

A = Control 6.33 ± 1.53 7.37 ± 1.00 a 13.84 ± 5.65 

B = Bacillus safensis strain MDL5 7.71 ± 1.05 9.33 ± 2.08 ab 13.64 ± 3.84 

C = Bacillus altitudinis strain RPW2 7.81 ± 0.77 11.03 ± 3.67 b 13.99 ± 5.66 

D = Bacillus subtilis strain YPS4 7.79 ± 2.08 10.56 ± 2.75 b 15.73 ± 2.60 

E = Bacillus sp. strain SZ057 8.57 ± 1.78 9.57 ± 1.52 ab 14.79 ± 4.01 

F = Bacillus consortium 8.03 ± 1.50 11.70 ± 2.45 b 17.87 ± 5.30 

 

 

The results of analysis of variance showed that Bacillus inoculation had a significant effect on broccoli plant 

height at 2 WAP (Table 1). Bacillus inoculation was able to increase plant height by 43.28–58.75% compared to the 

control, as shown in the inoculation treatment of Bacillus altitudinis strain RPW2, Bacillus subtilis strain YPS4, and 

Bacillus consortium. 

 

Table 2. Number of leaves of broccoli plant grown with bacillus inoculation at 1 – 3 weeks after planting (WAP). 

Treatment 
Number of leaves 

1 WAP 2 WAP 3 WAP 

A = Control 3.29 ± 0.76 4.29 ± 0.49 5.57 ± 1.27 

B = Bacillus safensis strain MDL5 3.29 ± 0.49 4.14 ± 0.69 5.71 ± 1.11 

C = Bacillus altitudinis strain RPW2 3.43 ± 0.98 4.43 ± 0.53 5.86 ± 1.21 

D = Bacillus subtilis strain YPS4 3.14 ± 0.69 4.14 ± 0.38 6.29 ± 1.70 

E = Bacillus sp. strain SZ057 3.43 ± 0.53 4.43 ± 0.53 6.14 ± 1.46 

F = Bacillus consortium 3.71 ± 0.95 4.71 ± 0.76 6.57 ± 0.79 

 

Note: Numbers followed by the same letter are not significantly different according to Duncan's multiple range test at the 
5% significance level. 

Note: Numbers followed by the same letter are not significantly different according to Duncan's multiple range test at 
the 5% significance level. 



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Analysis of variance showed that Bacillus inoculation had no significant effect on the number of broccoli plant 

leaves (Table 2). At 1-3 weeks after planting, the number of leaves of plants inoculated with Bacillus was almost the 

same as control plants. 

 

  
Figure 6. Stem diameter of broccoli plant grown with bacillus inoculation at 3 weeks after planting. 

 

The results of analysis of variance showed that Bacillus inoculation had no significant effect on stem diameter at 

3 WAP (Figure 6). There was no difference in stem diameter between the Bacillus inoculation treatment and the 

control. 

 

4. DISCUSSION 

Bacterial growth curves describe the dynamics of bacterial populations in a specific environment condition. 

Bacterial growth curves usually consist of several phases that reflect changes in bacterial numbers over time. The 

bacterial growth curve is divided into four main phases, namely the lag phase, exponential phase, stationary phase, 

and death phase [16]. The Bacillus population in this experiment is known to continue to increase until the 

exponential phase, then experience constant growth (stationary phase), and slowly decrease towards the death 

phase. The culture medium directly influences the growth rate, which is slower in nutrient-poor conditions and 

faster in nutrient-rich environments [17]. 

Bacillus is able to influence the pH of the growth medium due to several factors: the initial pH and composition 

of the medium, the growth phase of bacteria, and the physiology and optimum pH of the bacterium [18]. In this 

experiment, the pH of the medium decreased at T1. This can be caused by metabolites produced by Bacillus, such as 

indole acetic acid and amino acids. Next, the pH of the medium increases slowly. This increase is thought to be 

related to the carbon source in the media. Bacteria that use citrate carbon sources have the potential to cause the 

alkalinization of the medium [18].  

Bacillus inoculation significantly increased plant height [19, 20] at 2 WAP. The ability of Bacillus to provide 

plants with nutrients like N and P can explain this increase. Nitrogen is a constituent component of various plant 

molecules such as amino acids, chlorophyll, nucleic acids, adenosine triphosphate (ATP), and phytohormones [21, 

22]. The nutrient P in plants is known to play an important role in cell elongation, cell differentiation, and cell wall 

thickening [23]. Aside from that, Bacillus also produces plant growth hormones. Cytokinins take part in a variety of 

plant growth processes, including photosynthesis, chloroplast differentiation, cell division, regulation of leaf 

senescence, nutrient metabolism, and increasing shoot growth [24, 25]. Gibberellin is a hormone that is important 

for organ elongation and expansion through cell growth [13]. On the other hand, the number of plant leaves and 



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© 2024 Conscientia Beam. All Rights Reserved. 

stem diameter, which do not increase, are allegedly caused by the distribution of nutrients and photosynthate, which 

is not dominant there. 

 

5. CONCLUSION 

The experiments concluded that isolates of B. safensis strain MDL5, B. altitudinis strain RPW2, B. subtilis 

strain YPS4, and Bacillus sp. strain SZ057 reached the peak of the growth curve at T4 and T5, after which they 

slowly decreased. The pH of the medium increases slowly due to the use of carbon by the Bacillus. Bacillus 

inoculation into broccoli plants was able to increase plant height by 43.28–58.75% compared to the control because 

of its capacity to provide nutrients and produce phytohormones. Bacillus inoculation did not significantly increase 

the number of leaves or stem diameter.  

 
Funding: This research is supported by PT Pupuk Kujang (Grant number: 5200024201). 
Institutional Review Board Statement: Not applicable. 
Transparency: The authors state that the manuscript is honest, truthful, and transparent, that no key 
aspects of the investigation have been omitted, and that any differences from the study as planned have been 
clarified. This study followed all writing ethics. 
Competing Interests: The authors declare that they have no competing interests. 
Authors’ Contributions: All authors contributed equally to the conception and design of the study. All 
authors have read and agreed to the published version of the manuscript. 

 

REFERENCES 

[1] R. U. Syed et al., "Broccoli: A multi-faceted vegetable for health: An in-depth review of its nutritional attributes, 

antimicrobial abilities, and anti-inflammatory properties," Antibiotics, vol. 12, no. 7, p. 1157, 2023.  

https://doi.org/10.3390/antibiotics12071157 

[2] G. S. Nagraj, A. Chouksey, S. Jaiswal, and A. K. Jaiswal, Broccoli. In Nutritional composition and antioxidant properties of 

fruits and vegetables. Dublin: Academic Press, 2020. 

[3] B. N. Fitriatin, N. S. Ariani, H. P. Kusumo, and M. R. Setiawati, "The effect of hydrogel-based biofertilizer and p 

fertilizer on the growth and yield of corn on inceptisols from Jatinangor," Soilrens, vol. 20, no. 1, p. 1, 2022.  

https://doi.org/10.24198/soilrens.v20i1.41350 

[4] R. Hindersah, A. R. Syntianis, A. Setiawan, and R. Devnita, "Role of biofertilizer to increase caysim yield, n and p 

residues and their plant uptake," Agricultural, vol. 33, no. 2, pp. 93-102, 2021.  

https://doi.org/10.24246/agric.2021.v33.i2.p93-102 

[5] B. P. Kubheka, M. D. Laing, and K. S. Yobo, "Combinations of a biofertilizer with micro-dosed chemical fertilizers 

increased yield of maize in a high acid saturated soil," Rhizosphere, vol. 13, p. 100189, 2020.  

https://doi.org/10.1016/j.rhisph.2020.100189 

[6] S. C. Parija, Bacillus. In Textbook of Microbiology and Immunology. Singapore: Springer Nature Singapore. 

https://doi.org/10.1007/978-981-19-3315-8_29, 2023. 

[7] A. R. Khan et al., "Bacillus spp. as bioagents: Uses and application for sustainable agriculture," Biology, vol. 11, no. 12, 

p. 1763, 2022.  https://doi.org/10.3390/biology11121763 

[8] A. Saeid, E. Prochownik, and J. Dobrowolska-Iwanek, "Phosphorus solubilization by Bacillus species," Molecules, vol. 

23, no. 11, p. 2897, 2018.  https://doi.org/10.3390/molecules23112897 

[9] P. C. B. Turnbull, Bacillus. In Medical Microbiology, 4th ed. Galveston: University of Texas Medical Branch, 1996. 

[10] P. Setlow, "Germination of spores of Bacillus species: What we know and do not know," Journal of Bacteriology, vol. 

196, no. 7, pp. 1297-1305, 2014.  https://doi.org/10.1128/jb.01455-13 

[11] C. K. Odoh, "Plant growth promoting rhizobacteria (PGPR): A bioprotectant bioinoculant for sustainable agrobiology. 

A review," International Journal of Advanced Research in Biological Sciences, vol. 4, no. 5, pp. 123-142, 2017.  

https://doi.org/10.22192/ijarbs.2017.04.05.014 

https://doi.org/10.3390/antibiotics12071157
https://doi.org/10.24198/soilrens.v20i1.41350
https://doi.org/10.24246/agric.2021.v33.i2.p93-102
https://doi.org/10.1016/j.rhisph.2020.100189
https://doi.org/10.1007/978-981-19-3315-8_29
https://doi.org/10.3390/biology11121763
https://doi.org/10.3390/molecules23112897
https://doi.org/10.1128/jb.01455-13
https://doi.org/10.22192/ijarbs.2017.04.05.014


Current Research in Agricultural Sciences, 2024, 11(2): 56-63 

 

 
63 

© 2024 Conscientia Beam. All Rights Reserved. 

[12] D. Miljaković, J. Marinković, and S. Balešević-Tubić, "The significance of Bacillus spp. in disease suppression and 

growth promotion of field and vegetable crops," Microorganisms, vol. 8, no. 7, p. 1037, 2020.  

https://doi.org/10.3390/microorganisms8071037 

[13] J. Binenbaum, R. Weinstain, and E. Shani, "Gibberellin localization and transport in plants," Trends in Plant Science, 

vol. 23, no. 5, pp. 410-421, 2018.  https://doi.org/10.1016/j.tplants.2018.02.005 

[14] W. G. Brenner and T. Schmülling, "Summarizing and exploring data of a decade of cytokinin-related transcriptomics," 

Frontiers in Plant Science, vol. 6, p. 29, 2015.  https://doi.org/10.3389/fpls.2015.00029 

[15] T. Yoshida et al., "The role of abscisic acid signaling in maintaining the metabolic balance required for Arabidopsis 

growth under nonstress conditions," The Plant Cell, vol. 31, no. 1, pp. 84-105, 2019.  

https://doi.org/10.1105/tpc.18.00766 

[16] R. M. Maier and I. L. Pepper, Bacterial growth. In Environmental Microbiology. Elsevier. https://doi.org/10.1016/B978-

0-12-394626-3.00003-X, 2015. 

[17] P. Pletnev, I. Osterman, P. Sergiev, A. Bogdanov, and O. Dontsova, "Survival guide: Escherichia coli in the stationary 

phase," Acta Naturae, vol. 7, no. 4 (27), pp. 22-33, 2015.  https://doi.org/10.32607/20758251-2015-7-4-22-33 

[18] R. Sánchez-Clemente, M. I. Igeño, A. G. Población, M. I. Guijo, F. Merchán, and R. Blasco, "Study of pH changes in 

media during bacterial growth of several environmental strains," presented at the Environment, Green Technology, 

and Engineering International Conference, Basel Switzerland: MDPI, Oct. 2018, 2018. 

[19] B. Li et al., "Bacillus subtilis promotes cucumber growth and quality under higher nutrient solution by altering the 

Rhizospheric microbial community," Plants, vol. 12, no. 2, p. 298, 2023.  https://doi.org/10.3390/plants12020298 

[20] M. d. C. d. Fonseca et al., "Bacillus subtilis inoculation improves nutrient uptake and physiological activity in sugarcane 

under drought stress," Microorganisms, vol. 10, no. 4, p. 809, 2022.  https://doi.org/10.3390/microorganisms10040809 

[21] C. R. Frink, P. E. Waggoner, and J. H. Ausubel, "Nitrogen fertilizer: Retrospect and prospect," Proceedings of the 

National Academy of Sciences, vol. 96, no. 4, pp. 1175-1180, 1999.  https://doi.org/10.1073/pnas.96.4.1175 

[22] N. Crawford and B. Forde, "Molecular and developmental biology of inorganic nitrogen nutrition," The Arabidopsis 

Book, vol. 1, pp. e0011-e0011, 2002.  https://doi.org/10.1199/tab.0011 

[23] F. Khan et al., "Effect of different levels of nitrogen and phosphorus on the phenology and yield of maize varieties," 

American Journal of Plant Sciences, vol. 5, pp. 2582-2590, 2014.  https://doi.org/10.4236/ajps.2014.517272 

[24] S.-M. Li, H.-X. Zheng, X.-S. Zhang, and N. Sui, "Cytokinins as central regulators during plant growth and stress 

response," Plant Cell Reports, vol. 40, pp. 271-282, 2021.  https://doi.org/10.1007/s00299-020-02612-1 

[25] T. Arkhipova, E. Prinsen, S. Veselov, E. Martinenko, A. Melentiev, and G. Kudoyarova, "Cytokinin producing bacteria 

enhance plant growth in drying soil," Plant and Soil, vol. 292, pp. 305-315, 2007.  https://doi.org/10.1007/s11104-

007-9233-5 

 

 

 

 

 

 

 

 

 

 

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https://doi.org/10.3390/microorganisms8071037
https://doi.org/10.1016/j.tplants.2018.02.005
https://doi.org/10.3389/fpls.2015.00029
https://doi.org/10.1105/tpc.18.00766
https://doi.org/10.1016/B978-0-12-394626-3.00003-X
https://doi.org/10.1016/B978-0-12-394626-3.00003-X
https://doi.org/10.32607/20758251-2015-7-4-22-33
https://doi.org/10.3390/plants12020298
https://doi.org/10.3390/microorganisms10040809
https://doi.org/10.1073/pnas.96.4.1175
https://doi.org/10.1199/tab.0011
https://doi.org/10.4236/ajps.2014.517272
https://doi.org/10.1007/s00299-020-02612-1
https://doi.org/10.1007/s11104-007-9233-5
https://doi.org/10.1007/s11104-007-9233-5

