36 Dental Anthropology 2021 │ Volume 34│ Issue 02 In a forensic context, successful positive identifica- tion of human skeletal remains starts by determin- ing the biological profile of the individual: sex, age at death, ancestry, and stature. Sex is usually one of the first parameters to be estimated, not only because other parameters and methods are sex de- pendent, but also because, when a reliable sex esti- mation can be obtained from the skeletal remains, only two possible outcomes are provided, male or female, thus lowering the number of possible indi- viduals to whom the remains belong by approxi- mately one-half (Scheuer, 2002; Scheuer and Black, 2007). In skeletal elements, sex estimation is usual- ly obtained by studying the pelvis followed by the skull, as it is considered to yield the second highest percentage of sexual dimorphism; nonetheless, it was found, that postcranial metric measurements can perform better than those from the skull, so these should be applied when the pelvis is unavail- able (Spradley & Jantz, 2011). However, when both cranial and postcranial bones are absent or frag- mented, other elements need to be considered (Bruzek & Murail, 2006; Ubelaker, 2008; İşcan & Odontometric Patterns in Human Mandibular Molars for Sex Estimation in a Forensic Context Sofia F. Franco 1* , Álvaro Azevedo 2,3 , Vítor M. J. Matos 4 , Daniel Mongiovi 1 , Alexandra Teixeira 1 1 IINFACTS - Institute of Research and Advanced Training in Health Sciences and Technologies, Department of Sciences, University Institute of Health Sciences (IUCS), CESPU, CRL, Gandra, Portugal 2 Faculty of Dental Medicine, University of Porto, Portugal 3 EPIUnit – Institute of Public Health, University of Porto, Portugal 4 Research Centre for Anthropology and Health, Department of Life Sciences, University of Coimbra, Coimbra, Portugal ABSTRACT Sex estimation is an important step in the identification of human skeletal remains, since it reduces the number of potential matches to approximately one-half. The os coxae and skull are consid- ered the most dimorphic skeletal elements; however, when unavailable, teeth may be used alternatively. This study aims to evaluate the usefulness of specific odontometric parameters from the mandibular first molar – mesiolingual-distobuccal distance (MLDB), mesiobuccal-distolingual distance (MBDL) and mesiodistal distance (MD) - on sex estimation in a Portuguese sample composed of 135 mandibles (78 males; 57 females; ages atdeath ranging from 18 to 59 years old) selected amongst the 505 individuals of the Coimbra Identified Skeletal Collection (University of Coimbra). Since canines are considered the most accurate teeth for sexual assessment, comparison between first molar parameters and canines mesiodistal dimensions (MD) was performed. Statistical analysis showed sexual dimorphism in human first molars and cut-off points between male or female groups were determined. Using the first molar MLDB and MBDL, 60.7% and 65.2% of individuals were correctly classified, respectively. Highest sex estimation accuracy was achieved with canine MD, reaching 74.6%. Our results indicate that although mandibular molar dimensions are useful for sex estimation, the canine should be prioritized when available to perform this task. *Correspondence to: Sofia F. Franco Institute of Research and Advanced Train- ing in Health Sciences and Technologies, Department of Sciences University Institute of Health Sciences Gandra, Portugal E-mail: sofiafranco16@gmail.com Keywords: Sexual Dimorphism; Odontometry; Mandibular First Molar; Mandibular Canine; Forensic Odontology 37 Dental Anthropology 2021 │ Volume 34│ Issue 02 Steyn, 2013). In these circumstances, dentition can be a good alternative due to its hard, stable, and durable composition. Teeth are often well pre- served and can endure fire, decomposition, and severe trauma; furthermore, their analysis may contribute to the biological profile and its sexual dimorphism is represented by the differing teeth dimensions between males and females (Tabor & Schrader, 2010; Cardoza, 2011; Vishwakarma & Guha, 2011). The most common tooth dimensions for sexual estimation are the mesiodistal (MD) and buccolin- gual (BL) crown diameters, which are defined as the distance between the most mesial and most distal points of the crown, and the distance be- tween the most buccal and most lingual points of the tooth, respectively (White & Folkens, 2005; İşcan & Steyn, 2013; Kondo & Manabe, 2016). Alt- hough most studies apply these linear dimensions, other measures, such as the Mandibular Canine Index (MCI), have also been evaluated (Scott & Turner, 1988; Rao et al., 1989). Of the many studies performed worldwide, most identify the canine as the most sexually di- morphic tooth of the human dentition (Acharya & Mainali, 2007; Acharya & Mainali, 2008; Cardoso, 2008; Zorba et al., 2011; Angadi et al., 2013; Viciano et al., 2013; Khamis et al., 2014). This is one of the reasons why the MCI is so often applied. For exam- ple, Rao and collaborators (1989) obtained an over- all accuracy of 85.9% in sex estimation when using MCI. Nevertheless, since the degree of sexual di- morphism is population specific, some studies pre- sented low success when employing this tech- nique, which led to the focus being shifted to dif- ferent teeth and dental dimensions (White & Folkens, 2005; Zorba et al. 2012; İşcan & Steyn, 2013; Narang et al., 2015). The posterior teeth, normally having more than one root, are considered by some researchers a bet- ter choice over anterior teeth since the former are more strongly attached to the dental arch and less prone to suffer postmortem loss, thus being more frequently found in fragmentary remains (İşcan & Steyn, 2013; Zorba et al., 2013; Narang et al., 2015). On the other hand, the linear diameters are not as easily measured in molars as they are in anterior teeth. This fact, combined with the compromise of wear and attrition on the linear diameters, guided Hillson and colleagues (2005) to come up with al- ternative dimensions for the posterior dentition. These included the crown diagonals: the distance between the most mesiolingual and most distobuc- cal points of the crown (MLDB) and distance be- tween the most mesiobuccal and most distolingual points (MBDL). The disarticulation of the mandible from the skull is a consequence of the decomposition of the human body and since the temporomandibular joint is one of the first to disarticulate during de- cay, it is not uncommon to find an isolated mandi- ble in forensic contexts (Pinheiro, 2006; Cardoza, 2011; Cunha, 2014). In Portugal, there are very few studies on sexual dimorphism using teeth and none of them focus on the mesiodistal nor diagonal dimensions of the molars (Cardoso, 2008; Pereira et al., 2010; Gonçalves et al., 2014; Silva et al., 2016; Gouveia et al. 2017; Azevedo et al., 2019). There- fore, the aim of this study was to evaluate the sexu- al dimorphism in mandibular first molars using the mesiodistal and alternative diagonal dimen- sions and to compare it to the mandibular canine with the traditional mesiodistal distance, in a sam- ple from the Portuguese population. Materials and Methods The sample was selected amongst the 505 individ- uals which compose the Coimbra Identified Skele- tal Collection (20th century), housed at the Depart- ment of Life Sciences, University of Coimbra, ac- cording to the following selection criteria: Portu- guese nationality; age at death ranging between 18 and 59 years old; mandibles presenting at least one well preserved first molar showing minimal signs of attrition (occlusal wear). The resulting study base comprised 135 human mandibles, 78 (57.8%) belonging to males and 57 (42.2%) to females. The dimensions were taken in millimetres with a Mitutoyo Digimatic caliper, to the closest 0.01 mm. The following dimensions were measured on the left mandibular first molars: mesiodistal distance (MD), mesiolingual to distobuccal distance (MLDB), and mesiobuccal to distolingual distance (MBDL); when left teeth were absent, the antimere was used. Additionally, MD of right canines was taken for analysis and comparison with previous measures. All dimensions were measured accord- ing to the recommendations of Hillson and collabo- rators (2005). The first molar was measured in 78 male and 57 female mandibles. Only 59 out of the 135 mandi- bles had preserved canines (33 from males and 26 from females). All measurements were collected by an experienced observer, each taken in triplicate in non-consecutive order and separated in time, to avoid bias. The first value was eliminated, and the definitive value was obtained from the arithmetic average of the two last measurements. The intraob- server error was calculated with Bland-Altman analyses (Myles & Cui, 2007). The comparison be- 38 Dental Anthropology 2021 │ Volume 34│ Issue 02 tween sexes by the selected tooth sizes were per- formed by the independent sample t-test, after checking the Normality assumptions by Kolmogo- rov-Smirnov or Shapiro-Wilk tests. Receiver Oper- ating Characteristics (ROC) curves were applied to estimate the more accurate cut-off value for each dimension, which discriminate males from fe- males. Tooth measurements with a value under the cut-off point were considered to belong to female individuals, and values equal to or higher than the cut-off point to belong to male individuals. Each dimension has an associated correct sexual classifi- cation accuracy, given by the sensitivity value for correctly classifying males and specificity for fe- males. Since MLDB and MBDL were too correlated (multicollinearity), the joint performance of sex classification was assessed using a multivariate binary logistic regression with first molar MD and canine MD, by estimating the probability of an in- dividual being a female. The regression model was constructed by enter-method approach. The Omni- bus Test was used to check that the model holds an improvement with respect to the constant model. Additionally, goodness of fit was assessed by Hos- mer-Lemeshow statistical test, and the percentage of data variance explained by the model was quan- tified by R-Square based statistics (Cox & Snell and Nagelkerk). The predicted probabilities of being a female were then used as the classification varia- ble. For the statistical analysis, version 25.0 of SPSS software (Statistical Package for Social Sciences) was used. The level of significance was established at 5%. A p-value with Bonferroni correction for multi- ple comparisons (four dimensions) of <0.05 was considered to indicate statistical significance. Results By the Bland-Altman analysis there were no rele- vant differences for the four dimensions (MLDB, MBDL, Molar MD and Canine MD) between the second and the third measurements, which were not statistically significant (p>0.05). The maximum absolute mean difference between the second and the third measurements was 0.013mm registered in the Molar MD dimension. With 95% of confidence, the mean differences for all dimensions are lower than 0.03mm. Moreover, the Bland-Altman analy- sis did not reveal a relationship between the differ- ences obtained with the second and third measure- ments, and the magnitude of the measurements. It also revealed a non-dependence between the varia- tion of those differences and the magnitude of the measurements. Table 1 depicts the MLDB, MBDL, first Molar MD and Canine MD dimensions. All measure- ments were higher for males, and the mean differ- ences were statistically significant (p<0.05). In ab- solute terms, the larger differences (>0.41mm) were found in MD dimensions. Figure 1 shows the ROC curves obtained for MLDB and MBDL dimensions. The MBDL dimen- sion exhibits a larger area under the curve (AUC) than that of MLDB, regardless of the cut-off con- sidered. Figure 2 shows the ROC curves obtained for first molar MD and canine MD dimensions. This figure clearly illustrates that the AUC were similar for almost cut-offs. Table 2 presents the results of the AUC analysis obtained for each of the four dimensions. Interest- ingly, the AUC values are well above 0.5 for all variables, showing statistical significance in any case (p<0.05). Accordingly, as shown in Figures 1 and 2, Table 2 points out that the most discrimina- tive variables are first molar MD and canine MD with an AUC of 0.735 and 0.801, respectively. On the other hand, both MLDB and MBDL dimensions have much lower performance in sex discrimina- tion (AUC values below 0.71). The accuracy assessment in sex prediction was based on the optimal cut-offs (Table 3). Using the MBDL dimension which presented higher AUC than MLDB for all cut-offs, with the optimal cut-off of 11.44mm, 67.9% of the males were correctly clas- sified, whereas females were accurately identified Dimensions (mm) Female (n=57) Male (n=78) p 95% CI (Difference) Lower-Upper bond MLDB 11.18±0.55 11.51±0.56 0.004 0.14-0.52 MBDL 11.29±0.52 11.70±0.52 <0.002 0.23-0.59 First Molar MD 10.77±0.57089 11.19±0.56530 0.04 0.10-0.69 Canine MD 6.39±0.35408 6.83±0.47072 <0.002 0.23-0.66 Mean ±standard deviation; p represents the p-value with Bonferroni correction for four dimensions. Table 1. Descriptive statistics for MLDB, MBDL, first Molar MD, and Canine MD dimensions by sex. 39 Dental Anthropology 2021 │ Volume 34│ Issue 02 Figure 1. ROC curve analyses for the MLDB and MBDL dimensions. The identity line represents the curve with AUC equal to 0.5. Figure 2. ROC curve analyses for Molar MD and Canine MD dimensions. The identity line represents the curve with AUC equal to 0.5. 40 Dental Anthropology 2021 │ Volume 34│ Issue 02 in 61.4% of the cases. The corresponding overall accuracy was around 65%. As expected, a greater accuracy in sex estimation was obtained with the optimal cut-offs for the first molar MD and canine MD dimensions, respectively, 10.89mm and 6.54mm. For both dimensions, 72.7% of the males were correctly classified. Yet, the Canine MD pre- sented better accuracy to identify the females with 76.9% accuracy, as opposed to 65.4% with the first molar MD. The Canine MD showed an overall per- formance of approximately 75%, followed by the first Molar MD (overall accuracy around 70%). Multivariate binary logistic regression was then performed to assess the joint impact of the two pre- dictors (first molar MD and canine MD) in sex clas- sification. The Omnibus Test of model coefficient (χ2=15.502; df=2) was statistically significant, with p<0.001. Regarding the model goodness of fit, the result of the Hosmer-Lemeshow test indicates that the null hypothesis could not be rejected (χ2=11.812; df=8; p<0.16). The R Square based sta- tistics was found to be 31% (Cox & Snell statistics). Finally, the first molar coefficient was not shown to be statistically significant (p=0.644), and the coeffi- cient estimates for canine MD (B=-2.606; Standard Error =0.988; Wald = 6.957; df = 1; p=0.008) was found to be negative and statistically significant. So, when the first molar MD and canine MD were considered together, 69.2% of females and 69.7% of males were correctly classified, with the overall accuracy being 69.5%. Discussion Sexual assessment is one of the first steps in the process of the identity reconstruction. When skele- tal preservation is seriously compromised by taph- onomic and/or anthropic reasons, teeth are of great value for sex estimation due to their strength and durability (Scheuer, 2002; Bruzek & Murail, 2006; Scheuer and Black, 2007; Ubelaker, 2008; İşcan & Steyn, 2013; Scott et al., 2018). Most studies estimating the sex from teeth size focus on the canines as these are known as the most sexually dimorphic teeth. In addition, it is Dimensions AUC CI95% AUC p MLDB (mm) 0.668 0.577-0.759 0.004 MBDL (mm) 0.705 0.617-0.792 <0.002 First Molar MD (mm) 0.735 0.603-0.866 0.008 Canine MD (mm) 0.801 0.684-0.918 <0.002 Table 2. Area under the curve (AUC) and corresponding 95% confidence intervals (CI) for the MLDB, MBDL, first Molar MD and Canine MD dimensions. p represents the p-value for the null hypothesis that the true AUC is 0.5 with Bonferroni correction for four dimensions. Dimensions (Optimal cut-off) Accuracy Female Male Total MLDB (11.27mm) 30/57 (52.6%) 52/78 (66.7%) 82/135 (60.7%) MBDL (11.44mm) 35/57 (61.4%) 53/78 (67.9%) 88/135 (65.2%) First Molar MD (10.89mm) 17/26 (65.4%) 24/33 (72.7%) 41/59 (69.5%) Canine MD (6.54mm) First Molar MD+Canine MD* 20/26 (76.9%) 18/26 (69.2%) 24/33 (72.7%) 23/33 (69.7%) 44/59 (74.6%) 41/59 (69.5%) *Multivariate binary logistic regression; the Omnibus Test of model coefficient (χ2=15.502; df=2; p<0.001) Table 3. Overall accuracy in sex classification for the MLDB, MBDL, first Molar MD and Canine MD dimen- sions. 41 Dental Anthropology 2021 │ Volume 34│ Issue 02 considered the sturdiest and most durable tooth when faced with disease and trauma (Kaushal et al., 2003; White & Folkens, 2005), which explains why linear dimensions and other measures like crown indexes – such as the MCI – are so common- ly applied (Rao et al., 1989; Kaushal et al., 2004; Zorba et al., 2011; Khamis et al., 2014; Azevedo et al., 2019). On the other hand, its increased proba- bility of postmortem loss, compared to posterior teeth, due to its single root led researchers to start evaluating the sexual estimation accuracy in mo- lars (İşcan & Steyn, 2013; Narang et al., 2015). This study corroborates the results of previous research showing that the dentition exhibits sexual dimorphism. Statistically significant differences between males and females were found in mandib- ular first molars size. Furthermore, the use of mo- lar alternative dimensions to the mesiodistal and buccolingual parameters, such as the diagonal di- mensions, can be particularly useful when the teeth crowns are partially absent. In this regard, the MBDL dimension provided the highest overall estimation accuracy: 65.2% of correct classifications and an AUC of 70.5%. However, when considering all dimensions, Canine MD was the variable with the highest correct classification accuracy, namely 74.6% and an AUC of 80.1%. Both results are in accordance with the conclusions of studies from other populations, with diagonal dimensions hav- ing an accuracy range between 58.3% and 76.6% and mesiodistal dimensions a range between 63.9% and 85.8% (Karaman, 2006; Acharya & Mainali, 2009; Acharya et al., 2011; Zorba et al., 2013; Manchanda et al., 2015; Narang et al., 2015; Tabasum et al., 2017; Azevedo et al., 2019). When both MD variables were considered in a multivariate binary logistic regression, the accura- cy of sexual estimation was 69.5%, slightly lower than the value achieved with canine MD alone, and the first molar MD variable ceased to be statistical- ly significant in this situation, which reinforces the significance of the canine. Nonetheless, these two dimensions were found to explain 31% of the exist- ing variability between male and female individu- als, which further corroborates that sexual dimor- phism is present in the dentition. Results from the first mandibular molar diago- nal dimensions did not achieve a higher accuracy compared to the mesiodistal dimensions, leading to the conclusion that the canine should always be analysed when present. However, it is important to bear in mind that sample size was smaller when investigating the MD dimensions, which could have led to a slight bias of results. In this sense, the molars prove to be a good option when combined with other elements and as a corroborating meth- od. It is also important to mention that these re- sults only apply to a Portuguese population of the early 20th Century since sexual dimorphism is pop- ulation specific, as previously mentioned. Because sexual dimorphism varies across space and time, further studies should test this method in a con- temporaneous Portuguese population (21st Centu- ry) to enable the investigation of secular trends in these odontometric parameters in this specific pop- ulation. Conclusions The present results corroborate that the posterior dentition, namely odontometric parameters of the first mandibular molars, demonstrates sexual di- morphism in humans. The mesiodistal dimension of the canine was the variable that showed the highest sexual estimation accuracy, reaching levels of 74.6%. The diagonal dimensions of the molar, although less accurate, proved to be acceptable variables to be used in conjunction with other di- mensions when the canine is unavailable, or when partial destruction of the molar crowns makes it impossible to use other parameters. Additional research of these variables should be carried out in other populations, both to further validate the use- fulness of posterior teeth in forensic scenarios and to contribute to secular trend investigations in the dentition’s sexual dimorphism. 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