id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
mkg-33682	Bhanuwati, Areta Vania; Pakpahan, Alfred	Cytotoxic test of different solvents of soursop (Annona muricata) leaf extract against HSC-3 cell line	2022	7	.pdf	application/pdf	4814	254	54	This study will explore the cytotoxic activity of A. muricata in three solvents with different polarities, namely ethanol (polar), ethyl acetate (semi-polar) and hexane (nonpolar), against oral cancer cells in vitro. MATERIALS AND METHODS The materials used in this study were soursop (A. muricata) leaves obtained from Manoko solvents, such as ethanol, ethyl acetate and hexane; supporting materials such as phosphate buffer saline (PBS); cell counting kit-8 (CCK-8) reagent; dulbecco’s modified eagle medium-fetal bovine serum (DMEM-FBS) 10% as media for the cells and as a negative control; dimethyl sulfoxide (DMSO) 1% as solvent control, to dissolve each fraction of extract and to see whether the solvent contributes to the cytotoxic activity; DMSO 10% as a positive control to obtain the desired effect of the treatment, which is the cytotoxic effect, and as an antibiotic (penicillin-streptomycin) and antimycotic (amphotericin B) 1%. This research shows that three extracts of A. muricata have a moderate cytotoxicity against HSC-3 cancer cells, with the greatest cytotoxicity in the ethyl acetate extract (IC50 = 76.66 µg/mL).	cache/mkg-33682.pdf	txt/mkg-33682.txt