id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
mkg-58282	Widyantoro, Indira; Hirata, Isao; Kato, Koichi	A dilution method to increase the yield of engineered basic fibroblast growth factor	2025	5	.pdf	application/pdf	3287	198	48	Protein aggregation is influenced by many factors, including protein structure, chemical degradation, temperature and solution conditions.10 In this study, we attempted to optimize solution conditions by using a citrate buffer (pH 5.0) instead of a neutral PB and by adding 500 mM NaCl to buffer solutions. J. (Majalah Kedokteran Gigi) 2025 September; 58(3): 219–223 upon overexpression as a fusion protein with glutathione S-transferase.11,12 Furthermore, diluting protein solutions has been shown to enhance the recovery of soluble and active enzymes.13	cache/mkg-58282.pdf	txt/mkg-58282.txt