id	sid	tid	token	lemma	pos
mkg-58282	1	1	219219	219219	NUM
mkg-58282	1	2	dental	dental	ADJ
mkg-58282	1	3	journal	journal	NOUN
mkg-58282	1	4	(	(	PUNCT
mkg-58282	1	5	majalah	majalah	PROPN
mkg-58282	1	6	kedokteran	kedokteran	PROPN
mkg-58282	1	7	gigi	gigi	PROPN
mkg-58282	1	8	)	)	PUNCT
mkg-58282	1	9	2025	2025	NUM
mkg-58282	1	10	september	september	PROPN
mkg-58282	1	11	;	;	PUNCT
mkg-58282	1	12	58(3	58(3	NUM
mkg-58282	1	13	):	):	PUNCT
mkg-58282	1	14	219–223	219–223	NUM
mkg-58282	1	15	original	original	ADJ
mkg-58282	1	16	article	article	NOUN
mkg-58282	1	17	a	a	DET
mkg-58282	1	18	dilution	dilution	NOUN
mkg-58282	1	19	method	method	NOUN
mkg-58282	1	20	to	to	PART
mkg-58282	1	21	increase	increase	VERB
mkg-58282	1	22	the	the	DET
mkg-58282	1	23	yield	yield	NOUN
mkg-58282	1	24	of	of	ADP
mkg-58282	1	25	engineered	engineer	VERB
mkg-58282	1	26	basic	basic	ADJ
mkg-58282	1	27	fibroblast	fibroblast	ADJ
mkg-58282	1	28	growth	growth	NOUN
mkg-58282	1	29	factor	factor	NOUN
mkg-58282	1	30	indira	indira	PROPN
mkg-58282	1	31	widyantoro1,2	widyantoro1,2	PROPN
mkg-58282	1	32	,	,	PUNCT
mkg-58282	1	33	isao	isao	PROPN
mkg-58282	1	34	hirata1	hirata1	PROPN
mkg-58282	1	35	,	,	PUNCT
mkg-58282	1	36	and	and	CCONJ
mkg-58282	1	37	koichi	koichi	PROPN
mkg-58282	1	38	kato1,2,3	kato1,2,3	PROPN
mkg-58282	1	39	1department	1department	NUM
mkg-58282	1	40	of	of	ADP
mkg-58282	1	41	biomaterials	biomaterial	NOUN
mkg-58282	1	42	,	,	PUNCT
mkg-58282	1	43	graduate	graduate	NOUN
mkg-58282	1	44	school	school	NOUN
mkg-58282	1	45	of	of	ADP
mkg-58282	1	46	biomedical	biomedical	ADJ
mkg-58282	1	47	and	and	CCONJ
mkg-58282	1	48	health	health	NOUN
mkg-58282	1	49	sciences	sciences	PROPN
mkg-58282	1	50	,	,	PUNCT
mkg-58282	1	51	hiroshima	hiroshima	PROPN
mkg-58282	1	52	university	university	PROPN
mkg-58282	1	53	,	,	PUNCT
mkg-58282	1	54	hiroshima	hiroshima	PROPN
mkg-58282	1	55	,	,	PUNCT
mkg-58282	1	56	japan	japan	PROPN
mkg-58282	1	57	2faculty	2faculty	NUM
mkg-58282	1	58	of	of	ADP
mkg-58282	1	59	dental	dental	ADJ
mkg-58282	1	60	medicine	medicine	NOUN
mkg-58282	1	61	,	,	PUNCT
mkg-58282	1	62	airlangga	airlangga	PROPN
mkg-58282	1	63	university	university	PROPN
mkg-58282	1	64	,	,	PUNCT
mkg-58282	1	65	surabaya	surabaya	PROPN
mkg-58282	1	66	,	,	PUNCT
mkg-58282	1	67	indonesia	indonesia	PROPN
mkg-58282	1	68	3interdisciplinary	3interdisciplinary	NUM
mkg-58282	1	69	research	research	NOUN
mkg-58282	1	70	division	division	NOUN
mkg-58282	1	71	,	,	PUNCT
mkg-58282	1	72	research	research	NOUN
mkg-58282	1	73	institute	institute	NOUN
mkg-58282	1	74	for	for	ADP
mkg-58282	1	75	semiconductor	semiconductor	NOUN
mkg-58282	1	76	engineering	engineering	NOUN
mkg-58282	1	77	,	,	PUNCT
mkg-58282	1	78	hiroshima	hiroshima	PROPN
mkg-58282	1	79	university	university	PROPN
mkg-58282	1	80	,	,	PUNCT
mkg-58282	1	81	higashi	higashi	PROPN
mkg-58282	1	82	-	-	PUNCT
mkg-58282	1	83	hiroshima	hiroshima	PROPN
mkg-58282	1	84	,	,	PUNCT
mkg-58282	1	85	japan	japan	PROPN
mkg-58282	1	86	abstract	abstract	ADJ
mkg-58282	1	87	background	background	NOUN
mkg-58282	1	88	:	:	PUNCT
mkg-58282	1	89	genetically	genetically	ADV
mkg-58282	1	90	engineered	engineer	VERB
mkg-58282	1	91	chimeric	chimeric	ADJ
mkg-58282	1	92	proteins	protein	NOUN
mkg-58282	1	93	have	have	VERB
mkg-58282	1	94	potential	potential	ADJ
mkg-58282	1	95	applications	application	NOUN
mkg-58282	1	96	as	as	ADP
mkg-58282	1	97	components	component	NOUN
mkg-58282	1	98	of	of	ADP
mkg-58282	1	99	dental	dental	ADJ
mkg-58282	1	100	materials	material	NOUN
mkg-58282	1	101	and	and	CCONJ
mkg-58282	1	102	can	can	AUX
mkg-58282	1	103	be	be	AUX
mkg-58282	1	104	synthesized	synthesize	VERB
mkg-58282	1	105	recombinantly	recombinantly	ADV
mkg-58282	1	106	in	in	ADP
mkg-58282	1	107	various	various	ADJ
mkg-58282	1	108	cells	cell	NOUN
mkg-58282	1	109	,	,	PUNCT
mkg-58282	1	110	including	include	VERB
mkg-58282	1	111	bacteria	bacteria	NOUN
mkg-58282	1	112	,	,	PUNCT
mkg-58282	1	113	yeast	yeast	NOUN
mkg-58282	1	114	,	,	PUNCT
mkg-58282	1	115	insects	insect	NOUN
mkg-58282	1	116	and	and	CCONJ
mkg-58282	1	117	mammals	mammal	NOUN
mkg-58282	1	118	.	.	PUNCT
mkg-58282	2	1	however	however	ADV
mkg-58282	2	2	,	,	PUNCT
mkg-58282	2	3	increasing	increase	VERB
mkg-58282	2	4	the	the	DET
mkg-58282	2	5	yields	yield	NOUN
mkg-58282	2	6	of	of	ADP
mkg-58282	2	7	functionally	functionally	ADV
mkg-58282	2	8	active	active	ADJ
mkg-58282	2	9	products	product	NOUN
mkg-58282	2	10	remains	remain	VERB
mkg-58282	2	11	a	a	DET
mkg-58282	2	12	challenge	challenge	NOUN
mkg-58282	2	13	.	.	PUNCT
mkg-58282	3	1	purpose	purpose	NOUN
mkg-58282	3	2	:	:	PUNCT
mkg-58282	3	3	this	this	DET
mkg-58282	3	4	study	study	NOUN
mkg-58282	3	5	focused	focus	VERB
mkg-58282	3	6	on	on	ADP
mkg-58282	3	7	basic	basic	ADJ
mkg-58282	3	8	fibroblast	fibroblast	ADJ
mkg-58282	3	9	growth	growth	NOUN
mkg-58282	3	10	factor	factor	NOUN
mkg-58282	3	11	fused	fuse	VERB
mkg-58282	3	12	with	with	ADP
mkg-58282	3	13	pentadeca	pentadeca	NOUN
mkg-58282	3	14	(	(	PUNCT
mkg-58282	3	15	ggggs)3	ggggs)3	X
mkg-58282	3	16	peptide	peptide	NOUN
mkg-58282	3	17	as	as	ADP
mkg-58282	3	18	a	a	DET
mkg-58282	3	19	linker	linker	NOUN
mkg-58282	3	20	and	and	CCONJ
mkg-58282	3	21	hexahistidine	hexahistidine	NOUN
mkg-58282	3	22	as	as	ADP
mkg-58282	3	23	an	an	DET
mkg-58282	3	24	affinity	affinity	NOUN
mkg-58282	3	25	tag	tag	NOUN
mkg-58282	3	26	(	(	PUNCT
mkg-58282	3	27	bfgf	bfgf	NOUN
mkg-58282	3	28	-	-	PUNCT
mkg-58282	3	29	ph	ph	NOUN
mkg-58282	3	30	)	)	PUNCT
mkg-58282	3	31	.	.	PUNCT
mkg-58282	4	1	the	the	DET
mkg-58282	4	2	objective	objective	NOUN
mkg-58282	4	3	was	be	AUX
mkg-58282	4	4	to	to	PART
mkg-58282	4	5	enhance	enhance	VERB
mkg-58282	4	6	the	the	DET
mkg-58282	4	7	yield	yield	NOUN
mkg-58282	4	8	of	of	ADP
mkg-58282	4	9	bfgf	bfgf	NOUN
mkg-58282	4	10	-	-	PUNCT
mkg-58282	4	11	ph	ph	NOUN
mkg-58282	4	12	expressed	express	VERB
mkg-58282	4	13	in	in	ADP
mkg-58282	4	14	bacteria	bacteria	NOUN
mkg-58282	4	15	by	by	ADP
mkg-58282	4	16	employing	employ	VERB
mkg-58282	4	17	a	a	DET
mkg-58282	4	18	dilution	dilution	NOUN
mkg-58282	4	19	method	method	NOUN
mkg-58282	4	20	.	.	PUNCT
mkg-58282	5	1	methods	method	NOUN
mkg-58282	5	2	:	:	PUNCT
mkg-58282	5	3	escherichia	escherichia	NOUN
mkg-58282	5	4	coli	coli	NOUN
mkg-58282	5	5	was	be	AUX
mkg-58282	5	6	used	use	VERB
mkg-58282	5	7	to	to	PART
mkg-58282	5	8	express	express	VERB
mkg-58282	5	9	bfgf	bfgf	NOUN
mkg-58282	5	10	-	-	PUNCT
mkg-58282	5	11	ph	ph	NOUN
mkg-58282	5	12	in	in	ADP
mkg-58282	5	13	a	a	DET
mkg-58282	5	14	soluble	soluble	ADJ
mkg-58282	5	15	form	form	NOUN
mkg-58282	5	16	,	,	PUNCT
mkg-58282	5	17	which	which	PRON
mkg-58282	5	18	was	be	AUX
mkg-58282	5	19	then	then	ADV
mkg-58282	5	20	purified	purified	ADJ
mkg-58282	5	21	using	use	VERB
mkg-58282	5	22	metal	metal	NOUN
mkg-58282	5	23	chelate	chelate	NOUN
mkg-58282	5	24	affinity	affinity	NOUN
mkg-58282	5	25	chromatography	chromatography	NOUN
mkg-58282	5	26	.	.	PUNCT
mkg-58282	6	1	the	the	DET
mkg-58282	6	2	protein	protein	NOUN
mkg-58282	6	3	solution	solution	NOUN
mkg-58282	6	4	was	be	AUX
mkg-58282	6	5	diluted	dilute	VERB
mkg-58282	6	6	100	100	NUM
mkg-58282	6	7	-	-	PUNCT
mkg-58282	6	8	fold	fold	VERB
mkg-58282	6	9	with	with	ADP
mkg-58282	6	10	a	a	DET
mkg-58282	6	11	buffer	buffer	NOUN
mkg-58282	6	12	solution	solution	NOUN
mkg-58282	6	13	to	to	PART
mkg-58282	6	14	promote	promote	VERB
mkg-58282	6	15	spontaneous	spontaneous	ADJ
mkg-58282	6	16	refolding	refolding	NOUN
mkg-58282	6	17	.	.	PUNCT
mkg-58282	7	1	subsequently	subsequently	ADV
mkg-58282	7	2	,	,	PUNCT
mkg-58282	7	3	the	the	DET
mkg-58282	7	4	protein	protein	NOUN
mkg-58282	7	5	solution	solution	NOUN
mkg-58282	7	6	was	be	AUX
mkg-58282	7	7	concentrated	concentrate	VERB
mkg-58282	7	8	using	use	VERB
mkg-58282	7	9	metal	metal	NOUN
mkg-58282	7	10	chelate	chelate	NOUN
mkg-58282	7	11	affinity	affinity	NOUN
mkg-58282	7	12	chromatography	chromatography	NOUN
mkg-58282	7	13	.	.	PUNCT
mkg-58282	8	1	circular	circular	ADJ
mkg-58282	8	2	dichroism	dichroism	NOUN
mkg-58282	8	3	(	(	PUNCT
mkg-58282	8	4	cd	cd	NOUN
mkg-58282	8	5	)	)	PUNCT
mkg-58282	8	6	spectroscopy	spectroscopy	NOUN
mkg-58282	8	7	was	be	AUX
mkg-58282	8	8	used	use	VERB
mkg-58282	8	9	to	to	PART
mkg-58282	8	10	analyze	analyze	VERB
mkg-58282	8	11	the	the	DET
mkg-58282	8	12	protein	protein	NOUN
mkg-58282	8	13	’s	’s	PART
mkg-58282	8	14	structure	structure	NOUN
mkg-58282	8	15	,	,	PUNCT
mkg-58282	8	16	assessing	assess	VERB
mkg-58282	8	17	its	its	PRON
mkg-58282	8	18	correct	correct	ADJ
mkg-58282	8	19	folding	folding	NOUN
mkg-58282	8	20	by	by	ADP
mkg-58282	8	21	comparing	compare	VERB
mkg-58282	8	22	it	it	PRON
mkg-58282	8	23	to	to	ADP
mkg-58282	8	24	a	a	DET
mkg-58282	8	25	reference	reference	NOUN
mkg-58282	8	26	spectrum	spectrum	NOUN
mkg-58282	8	27	obtained	obtain	VERB
mkg-58282	8	28	through	through	ADP
mkg-58282	8	29	computer	computer	NOUN
mkg-58282	8	30	-	-	PUNCT
mkg-58282	8	31	based	base	VERB
mkg-58282	8	32	simulation	simulation	NOUN
mkg-58282	8	33	.	.	PUNCT
mkg-58282	9	1	results	result	NOUN
mkg-58282	9	2	:	:	PUNCT
mkg-58282	9	3	the	the	DET
mkg-58282	9	4	dilution	dilution	NOUN
mkg-58282	9	5	method	method	NOUN
mkg-58282	9	6	prevented	prevent	VERB
mkg-58282	9	7	bfgf	bfgf	ADJ
mkg-58282	9	8	-	-	PUNCT
mkg-58282	9	9	ph	ph	ADJ
mkg-58282	9	10	aggregation	aggregation	NOUN
mkg-58282	9	11	,	,	PUNCT
mkg-58282	9	12	and	and	CCONJ
mkg-58282	9	13	cd	cd	PROPN
mkg-58282	9	14	spectroscopy	spectroscopy	NOUN
mkg-58282	9	15	suggested	suggest	VERB
mkg-58282	9	16	that	that	SCONJ
mkg-58282	9	17	the	the	DET
mkg-58282	9	18	protein	protein	NOUN
mkg-58282	9	19	was	be	AUX
mkg-58282	9	20	correctly	correctly	ADV
mkg-58282	9	21	folded	fold	VERB
mkg-58282	9	22	.	.	PUNCT
mkg-58282	10	1	as	as	ADP
mkg-58282	10	2	a	a	DET
mkg-58282	10	3	result	result	NOUN
mkg-58282	10	4	,	,	PUNCT
mkg-58282	10	5	a	a	DET
mkg-58282	10	6	total	total	NOUN
mkg-58282	10	7	of	of	ADP
mkg-58282	10	8	3.0	3.0	NUM
mkg-58282	10	9	mg	mg	NOUN
mkg-58282	10	10	of	of	ADP
mkg-58282	10	11	bfgf	bfgf	NOUN
mkg-58282	10	12	-	-	PUNCT
mkg-58282	10	13	ph	ph	NOUN
mkg-58282	10	14	was	be	AUX
mkg-58282	10	15	obtained	obtain	VERB
mkg-58282	10	16	per	per	ADP
mkg-58282	10	17	liter	liter	NOUN
mkg-58282	10	18	of	of	ADP
mkg-58282	10	19	lysogeny	lysogeny	NOUN
mkg-58282	10	20	broth	broth	NOUN
mkg-58282	10	21	medium	medium	NOUN
mkg-58282	10	22	,	,	PUNCT
mkg-58282	10	23	which	which	PRON
mkg-58282	10	24	was	be	AUX
mkg-58282	10	25	higher	high	ADJ
mkg-58282	10	26	than	than	ADP
mkg-58282	10	27	the	the	DET
mkg-58282	10	28	yield	yield	NOUN
mkg-58282	10	29	achieved	achieve	VERB
mkg-58282	10	30	using	use	VERB
mkg-58282	10	31	the	the	DET
mkg-58282	10	32	conventional	conventional	ADJ
mkg-58282	10	33	method	method	NOUN
mkg-58282	10	34	.	.	PUNCT
mkg-58282	11	1	conclusion	conclusion	NOUN
mkg-58282	11	2	:	:	PUNCT
mkg-58282	11	3	the	the	DET
mkg-58282	11	4	dilution	dilution	NOUN
mkg-58282	11	5	method	method	NOUN
mkg-58282	11	6	examined	examine	VERB
mkg-58282	11	7	in	in	ADP
mkg-58282	11	8	this	this	DET
mkg-58282	11	9	study	study	NOUN
mkg-58282	11	10	increased	increase	VERB
mkg-58282	11	11	the	the	DET
mkg-58282	11	12	yield	yield	NOUN
mkg-58282	11	13	of	of	ADP
mkg-58282	11	14	correctly	correctly	ADV
mkg-58282	11	15	folded	fold	VERB
mkg-58282	11	16	bfgf	bfgf	NOUN
mkg-58282	11	17	-	-	PUNCT
mkg-58282	11	18	ph	ph	ADJ
mkg-58282	11	19	.	.	PUNCT
mkg-58282	11	20	keywords	keyword	NOUN
mkg-58282	11	21	:	:	PUNCT
mkg-58282	11	22	dental	dental	ADJ
mkg-58282	11	23	materials	material	NOUN
mkg-58282	11	24	;	;	PUNCT
mkg-58282	11	25	tissue	tissue	NOUN
mkg-58282	11	26	engineering	engineering	NOUN
mkg-58282	11	27	;	;	PUNCT
mkg-58282	11	28	recombinant	recombinant	ADJ
mkg-58282	11	29	protein	protein	NOUN
mkg-58282	11	30	;	;	PUNCT
mkg-58282	11	31	genetic	genetic	ADJ
mkg-58282	11	32	engineering	engineering	NOUN
mkg-58282	11	33	;	;	PUNCT
mkg-58282	11	34	protein	protein	NOUN
mkg-58282	11	35	aggregation	aggregation	NOUN
mkg-58282	11	36	;	;	PUNCT
mkg-58282	11	37	medicine	medicine	NOUN
mkg-58282	11	38	article	article	NOUN
mkg-58282	11	39	history	history	NOUN
mkg-58282	11	40	:	:	PUNCT
mkg-58282	11	41	received	receive	VERB
mkg-58282	11	42	31	31	NUM
mkg-58282	11	43	may	may	PROPN
mkg-58282	11	44	2024	2024	NUM
mkg-58282	11	45	;	;	PUNCT
mkg-58282	11	46	revised	revise	VERB
mkg-58282	11	47	11	11	NUM
mkg-58282	11	48	july	july	PROPN
mkg-58282	11	49	2024	2024	NUM
mkg-58282	11	50	;	;	PUNCT
mkg-58282	11	51	accepted	accept	VERB
mkg-58282	11	52	31	31	NUM
mkg-58282	11	53	july	july	PROPN
mkg-58282	11	54	2024	2024	NUM
mkg-58282	11	55	;	;	PUNCT
mkg-58282	11	56	online	online	ADJ
mkg-58282	11	57	10	10	NUM
mkg-58282	11	58	may	may	AUX
mkg-58282	11	59	2025	2025	NUM
mkg-58282	11	60	correspondence	correspondence	NOUN
mkg-58282	11	61	:	:	PUNCT
mkg-58282	11	62	koichi	koichi	PROPN
mkg-58282	11	63	kato	kato	PROPN
mkg-58282	11	64	,	,	PUNCT
mkg-58282	11	65	department	department	NOUN
mkg-58282	11	66	of	of	ADP
mkg-58282	11	67	biomaterials	biomaterial	NOUN
mkg-58282	11	68	,	,	PUNCT
mkg-58282	11	69	graduate	graduate	NOUN
mkg-58282	11	70	school	school	NOUN
mkg-58282	11	71	of	of	ADP
mkg-58282	11	72	biomedical	biomedical	ADJ
mkg-58282	11	73	and	and	CCONJ
mkg-58282	11	74	health	health	NOUN
mkg-58282	11	75	sciences	sciences	PROPN
mkg-58282	11	76	,	,	PUNCT
mkg-58282	11	77	hiroshima	hiroshima	PROPN
mkg-58282	11	78	university	university	PROPN
mkg-58282	11	79	,	,	PUNCT
mkg-58282	11	80	1	1	NUM
mkg-58282	11	81	-	-	SYM
mkg-58282	11	82	2	2	NUM
mkg-58282	11	83	-	-	SYM
mkg-58282	11	84	3	3	NUM
mkg-58282	11	85	kasumi	kasumi	PROPN
mkg-58282	11	86	,	,	PUNCT
mkg-58282	11	87	minami	minami	PROPN
mkg-58282	11	88	-	-	PUNCT
mkg-58282	11	89	ku	ku	PROPN
mkg-58282	11	90	,	,	PUNCT
mkg-58282	11	91	hiroshima	hiroshima	PROPN
mkg-58282	11	92	734	734	NUM
mkg-58282	11	93	-	-	SYM
mkg-58282	11	94	8553	8553	NUM
mkg-58282	11	95	,	,	PUNCT
mkg-58282	11	96	japan	japan	PROPN
mkg-58282	11	97	.	.	PUNCT
mkg-58282	12	1	email	email	NOUN
mkg-58282	12	2	:	:	PUNCT
mkg-58282	12	3	kokato@hiroshima-u.ac.jp	kokato@hiroshima-u.ac.jp	NOUN
mkg-58282	12	4	introduction	introduction	NOUN
mkg-58282	12	5	chimeric	chimeric	ADJ
mkg-58282	12	6	proteins	protein	NOUN
mkg-58282	12	7	consist	consist	NOUN
mkg-58282	12	8	of	of	ADP
mkg-58282	12	9	two	two	NUM
mkg-58282	12	10	or	or	CCONJ
mkg-58282	12	11	more	more	ADJ
mkg-58282	12	12	tandem	tandem	NOUN
mkg-58282	12	13	polypeptides	polypeptide	NOUN
mkg-58282	12	14	,	,	PUNCT
mkg-58282	12	15	each	each	PRON
mkg-58282	12	16	with	with	ADP
mkg-58282	12	17	distinct	distinct	ADJ
mkg-58282	12	18	biofunctionalities	biofunctionalitie	NOUN
mkg-58282	12	19	.	.	PUNCT
mkg-58282	13	1	they	they	PRON
mkg-58282	13	2	have	have	VERB
mkg-58282	13	3	potential	potential	ADJ
mkg-58282	13	4	applications	application	NOUN
mkg-58282	13	5	as	as	ADP
mkg-58282	13	6	components	component	NOUN
mkg-58282	13	7	of	of	ADP
mkg-58282	13	8	dental	dental	ADJ
mkg-58282	13	9	materials	material	NOUN
mkg-58282	13	10	,	,	PUNCT
mkg-58282	13	11	particularly	particularly	ADV
mkg-58282	13	12	in	in	ADP
mkg-58282	13	13	tissue	tissue	NOUN
mkg-58282	13	14	engineering	engineering	NOUN
mkg-58282	13	15	scaffolds	scaffold	NOUN
mkg-58282	13	16	for	for	ADP
mkg-58282	13	17	the	the	DET
mkg-58282	13	18	restoration	restoration	NOUN
mkg-58282	13	19	of	of	ADP
mkg-58282	13	20	bone	bone	NOUN
mkg-58282	13	21	defects	defect	NOUN
mkg-58282	13	22	caused	cause	VERB
mkg-58282	13	23	by	by	ADP
mkg-58282	13	24	tumor	tumor	NOUN
mkg-58282	13	25	resection	resection	NOUN
mkg-58282	13	26	and	and	CCONJ
mkg-58282	13	27	periodontitis	periodontitis	NOUN
mkg-58282	13	28	-	-	PUNCT
mkg-58282	13	29	associated	associate	VERB
mkg-58282	13	30	bone	bone	NOUN
mkg-58282	13	31	resorption.1–5	resorption.1–5	NOUN
mkg-58282	13	32	additionally	additionally	ADV
mkg-58282	13	33	,	,	PUNCT
mkg-58282	13	34	chimeric	chimeric	ADJ
mkg-58282	13	35	proteins	protein	NOUN
mkg-58282	13	36	with	with	ADP
mkg-58282	13	37	growth	growth	NOUN
mkg-58282	13	38	factor	factor	NOUN
mkg-58282	13	39	-	-	PUNCT
mkg-58282	13	40	like	like	ADJ
mkg-58282	13	41	biofunctionality	biofunctionality	NOUN
mkg-58282	13	42	and	and	CCONJ
mkg-58282	13	43	the	the	DET
mkg-58282	13	44	ability	ability	NOUN
mkg-58282	13	45	to	to	PART
mkg-58282	13	46	bind	bind	VERB
mkg-58282	13	47	to	to	ADP
mkg-58282	13	48	solid	solid	ADJ
mkg-58282	13	49	substrates	substrate	NOUN
mkg-58282	13	50	can	can	AUX
mkg-58282	13	51	be	be	AUX
mkg-58282	13	52	used	use	VERB
mkg-58282	13	53	to	to	PART
mkg-58282	13	54	fabricate	fabricate	VERB
mkg-58282	13	55	biologically	biologically	ADV
mkg-58282	13	56	active	active	ADJ
mkg-58282	13	57	materials	material	NOUN
mkg-58282	13	58	beneficial	beneficial	ADJ
mkg-58282	13	59	in	in	ADP
mkg-58282	13	60	stem	stem	NOUN
mkg-58282	13	61	cell	cell	NOUN
mkg-58282	13	62	engineering.6,7	engineering.6,7	NOUN
mkg-58282	13	63	these	these	DET
mkg-58282	13	64	applications	application	NOUN
mkg-58282	13	65	rely	rely	VERB
mkg-58282	13	66	on	on	ADP
mkg-58282	13	67	recombinant	recombinant	ADJ
mkg-58282	13	68	protein	protein	NOUN
mkg-58282	13	69	synthesis	synthesis	NOUN
mkg-58282	13	70	technologies	technology	NOUN
mkg-58282	13	71	.	.	PUNCT
mkg-58282	14	1	recombinant	recombinant	ADJ
mkg-58282	14	2	chimeric	chimeric	ADJ
mkg-58282	14	3	proteins	protein	NOUN
mkg-58282	14	4	can	can	AUX
mkg-58282	14	5	be	be	AUX
mkg-58282	14	6	expressed	express	VERB
mkg-58282	14	7	in	in	ADP
mkg-58282	14	8	various	various	ADJ
mkg-58282	14	9	cells	cell	NOUN
mkg-58282	14	10	,	,	PUNCT
mkg-58282	14	11	including	include	VERB
mkg-58282	14	12	bacteria	bacteria	NOUN
mkg-58282	14	13	,	,	PUNCT
mkg-58282	14	14	yeast	yeast	NOUN
mkg-58282	14	15	,	,	PUNCT
mkg-58282	14	16	insects	insect	NOUN
mkg-58282	14	17	and	and	CCONJ
mkg-58282	14	18	mammals	mammal	NOUN
mkg-58282	14	19	.	.	PUNCT
mkg-58282	15	1	among	among	ADP
mkg-58282	15	2	these	these	PRON
mkg-58282	15	3	,	,	PUNCT
mkg-58282	15	4	bacterial	bacterial	ADJ
mkg-58282	15	5	expression	expression	NOUN
mkg-58282	15	6	systems	system	NOUN
mkg-58282	15	7	are	be	AUX
mkg-58282	15	8	the	the	DET
mkg-58282	15	9	most	most	ADV
mkg-58282	15	10	straightforward	straightforward	ADJ
mkg-58282	15	11	and	and	CCONJ
mkg-58282	15	12	efficient	efficient	ADJ
mkg-58282	15	13	.	.	PUNCT
mkg-58282	16	1	however	however	ADV
mkg-58282	16	2	,	,	PUNCT
mkg-58282	16	3	exogenous	exogenous	ADJ
mkg-58282	16	4	proteins	protein	NOUN
mkg-58282	16	5	are	be	AUX
mkg-58282	16	6	often	often	ADV
mkg-58282	16	7	deposited	deposit	VERB
mkg-58282	16	8	in	in	ADP
mkg-58282	16	9	cytosolic	cytosolic	ADJ
mkg-58282	16	10	inclusion	inclusion	NOUN
mkg-58282	16	11	bodies	body	NOUN
mkg-58282	16	12	,	,	PUNCT
mkg-58282	16	13	necessitating	necessitate	VERB
mkg-58282	16	14	solubilization	solubilization	NOUN
mkg-58282	16	15	with	with	ADP
mkg-58282	16	16	chaotropic	chaotropic	NOUN
mkg-58282	16	17	agents	agent	NOUN
mkg-58282	16	18	such	such	ADJ
mkg-58282	16	19	as	as	ADP
mkg-58282	16	20	urea	urea	NOUN
mkg-58282	16	21	and	and	CCONJ
mkg-58282	16	22	guanidine	guanidine	ADJ
mkg-58282	16	23	hydrochloride	hydrochloride	NOUN
mkg-58282	16	24	.	.	PUNCT
mkg-58282	17	1	to	to	PART
mkg-58282	17	2	obtain	obtain	VERB
mkg-58282	17	3	biologically	biologically	ADV
mkg-58282	17	4	active	active	ADJ
mkg-58282	17	5	products	product	NOUN
mkg-58282	17	6	,	,	PUNCT
mkg-58282	17	7	dialysis	dialysis	NOUN
mkg-58282	17	8	is	be	AUX
mkg-58282	17	9	conventionally	conventionally	ADV
mkg-58282	17	10	performed	perform	VERB
mkg-58282	17	11	to	to	PART
mkg-58282	17	12	remove	remove	VERB
mkg-58282	17	13	these	these	DET
mkg-58282	17	14	denaturing	denature	VERB
mkg-58282	17	15	agents	agent	NOUN
mkg-58282	17	16	and	and	CCONJ
mkg-58282	17	17	refold	refold	VERB
mkg-58282	17	18	the	the	DET
mkg-58282	17	19	proteins	protein	NOUN
mkg-58282	17	20	.	.	PUNCT
mkg-58282	18	1	even	even	ADV
mkg-58282	18	2	when	when	SCONJ
mkg-58282	18	3	proteins	protein	NOUN
mkg-58282	18	4	are	be	AUX
mkg-58282	18	5	expressed	express	VERB
mkg-58282	18	6	in	in	ADP
mkg-58282	18	7	bacteria	bacteria	NOUN
mkg-58282	18	8	in	in	ADP
mkg-58282	18	9	a	a	DET
mkg-58282	18	10	soluble	soluble	ADJ
mkg-58282	18	11	form	form	NOUN
mkg-58282	18	12	,	,	PUNCT
mkg-58282	18	13	misfolding	misfolding	NOUN
mkg-58282	18	14	and	and	CCONJ
mkg-58282	18	15	aggregation	aggregation	NOUN
mkg-58282	18	16	frequently	frequently	ADV
mkg-58282	18	17	occur	occur	VERB
mkg-58282	18	18	during	during	ADP
mkg-58282	18	19	dialysis	dialysis	NOUN
mkg-58282	18	20	for	for	ADP
mkg-58282	18	21	buffer	buffer	NOUN
mkg-58282	18	22	exchange	exchange	NOUN
mkg-58282	18	23	.	.	PUNCT
mkg-58282	19	1	the	the	DET
mkg-58282	19	2	misfolding	misfolding	NOUN
mkg-58282	19	3	and	and	CCONJ
mkg-58282	19	4	aggregation	aggregation	NOUN
mkg-58282	19	5	of	of	ADP
mkg-58282	19	6	proteins	protein	NOUN
mkg-58282	19	7	considerably	considerably	ADV
mkg-58282	19	8	decrease	decrease	VERB
mkg-58282	19	9	the	the	DET
mkg-58282	19	10	yield	yield	NOUN
mkg-58282	19	11	of	of	ADP
mkg-58282	19	12	active	active	ADJ
mkg-58282	19	13	products	product	NOUN
mkg-58282	19	14	.	.	PUNCT
mkg-58282	20	1	numerous	numerous	ADJ
mkg-58282	20	2	studies	study	NOUN
mkg-58282	20	3	have	have	AUX
mkg-58282	20	4	been	be	AUX
mkg-58282	20	5	conducted	conduct	VERB
mkg-58282	20	6	to	to	PART
mkg-58282	20	7	overcome	overcome	VERB
mkg-58282	20	8	this	this	DET
mkg-58282	20	9	problem,8	problem,8	NOUN
mkg-58282	20	10	including	include	VERB
mkg-58282	20	11	the	the	DET
mkg-58282	20	12	use	use	NOUN
mkg-58282	20	13	of	of	ADP
mkg-58282	20	14	additives9	additives9	NOUN
mkg-58282	20	15	such	such	ADJ
mkg-58282	20	16	as	as	ADP
mkg-58282	20	17	sucrose	sucrose	NOUN
mkg-58282	20	18	and	and	CCONJ
mkg-58282	20	19	arginine	arginine	VERB
mkg-58282	20	20	chloride	chloride	NOUN
mkg-58282	20	21	and	and	CCONJ
mkg-58282	20	22	stabilizers10	stabilizers10	NOUN
mkg-58282	20	23	such	such	ADJ
mkg-58282	20	24	as	as	ADP
mkg-58282	20	25	polysorbates	polysorbate	NOUN
mkg-58282	20	26	and	and	CCONJ
mkg-58282	20	27	pluronic	pluronic	ADJ
mkg-58282	20	28	to	to	PART
mkg-58282	20	29	prevent	prevent	VERB
mkg-58282	20	30	protein	protein	NOUN
mkg-58282	20	31	aggregation	aggregation	NOUN
mkg-58282	20	32	.	.	PUNCT
mkg-58282	21	1	other	other	ADJ
mkg-58282	21	2	groups	group	NOUN
mkg-58282	21	3	have	have	AUX
mkg-58282	21	4	reported	report	VERB
mkg-58282	21	5	that	that	SCONJ
mkg-58282	21	6	the	the	DET
mkg-58282	21	7	solubility	solubility	NOUN
mkg-58282	21	8	of	of	ADP
mkg-58282	21	9	a	a	DET
mkg-58282	21	10	recombinant	recombinant	ADJ
mkg-58282	21	11	protein	protein	NOUN
mkg-58282	21	12	increased	increase	VERB
mkg-58282	21	13	copyright	copyright	NOUN
mkg-58282	21	14	©	©	ADP
mkg-58282	21	15	2025	2025	NUM
mkg-58282	21	16	dental	dental	ADJ
mkg-58282	21	17	journal	journal	NOUN
mkg-58282	21	18	(	(	PUNCT
mkg-58282	21	19	majalah	majalah	PROPN
mkg-58282	21	20	kedokteran	kedokteran	PROPN
mkg-58282	21	21	gigi	gigi	PROPN
mkg-58282	21	22	)	)	PUNCT
mkg-58282	22	1	p	p	X
mkg-58282	22	2	-	-	PUNCT
mkg-58282	22	3	issn	issn	NOUN
mkg-58282	22	4	:	:	PUNCT
mkg-58282	22	5	1978	1978	NUM
mkg-58282	22	6	-	-	SYM
mkg-58282	22	7	3728	3728	NUM
mkg-58282	22	8	;	;	PUNCT
mkg-58282	22	9	e	e	X
mkg-58282	22	10	-	-	PUNCT
mkg-58282	22	11	issn	issn	ADJ
mkg-58282	22	12	:	:	PUNCT
mkg-58282	22	13	2442	2442	NUM
mkg-58282	22	14	-	-	SYM
mkg-58282	22	15	9740	9740	NUM
mkg-58282	22	16	.	.	PUNCT
mkg-58282	23	1	accredited	accredit	VERB
mkg-58282	23	2	no	no	INTJ
mkg-58282	23	3	.	.	PROPN
mkg-58282	24	1	158	158	NUM
mkg-58282	24	2	/	/	SYM
mkg-58282	24	3	e	e	NOUN
mkg-58282	24	4	/	/	SYM
mkg-58282	24	5	kpt/2021	kpt/2021	NOUN
mkg-58282	24	6	.	.	PUNCT
mkg-58282	25	1	open	open	ADJ
mkg-58282	25	2	access	access	NOUN
mkg-58282	25	3	under	under	ADP
mkg-58282	25	4	cc	cc	NOUN
mkg-58282	25	5	-	-	PUNCT
mkg-58282	25	6	by	by	ADP
mkg-58282	25	7	-	-	PUNCT
mkg-58282	25	8	sa	sa	NOUN
mkg-58282	25	9	license	license	NOUN
mkg-58282	25	10	.	.	PUNCT
mkg-58282	26	1	available	available	ADJ
mkg-58282	26	2	at	at	ADP
mkg-58282	26	3	https://e-journal.unair.ac.id/mkg/index	https://e-journal.unair.ac.id/mkg/index	NOUN
mkg-58282	26	4	doi	doi	NOUN
mkg-58282	26	5	:	:	PUNCT
mkg-58282	26	6	10.20473	10.20473	NUM
mkg-58282	26	7	/	/	SYM
mkg-58282	26	8	j.djmkg.v58.i3.p219–223	j.djmkg.v58.i3.p219–223	NOUN
mkg-58282	26	9	mailto:kokato@hiroshima-u.ac.jp	mailto:kokato@hiroshima-u.ac.jp	NOUN
mkg-58282	26	10	https://e-journal.unair.ac.id/mkg/index	https://e-journal.unair.ac.id/mkg/index	NOUN
mkg-58282	26	11	https://doi.org/10.20473/j.djmkg.v58.i3.p219-223	https://doi.org/10.20473/j.djmkg.v58.i3.p219-223	VERB
mkg-58282	26	12	220	220	NUM
mkg-58282	26	13	widyantoro	widyantoro	NOUN
mkg-58282	26	14	et	et	PROPN
mkg-58282	26	15	al	al	PROPN
mkg-58282	26	16	.	.	PROPN
mkg-58282	26	17	dent	dent	PROPN
mkg-58282	26	18	.	.	PUNCT
mkg-58282	27	1	j.	j.	PROPN
mkg-58282	27	2	(	(	PUNCT
mkg-58282	27	3	majalah	majalah	PROPN
mkg-58282	27	4	kedokteran	kedokteran	PROPN
mkg-58282	27	5	gigi	gigi	PROPN
mkg-58282	27	6	)	)	PUNCT
mkg-58282	27	7	2025	2025	NUM
mkg-58282	27	8	september	september	PROPN
mkg-58282	27	9	;	;	PUNCT
mkg-58282	27	10	58(3	58(3	NUM
mkg-58282	27	11	):	):	PUNCT
mkg-58282	27	12	219–223	219–223	NUM
mkg-58282	27	13	upon	upon	SCONJ
mkg-58282	27	14	overexpression	overexpression	NOUN
mkg-58282	27	15	as	as	ADP
mkg-58282	27	16	a	a	DET
mkg-58282	27	17	fusion	fusion	NOUN
mkg-58282	27	18	protein	protein	NOUN
mkg-58282	27	19	with	with	ADP
mkg-58282	27	20	glutathione	glutathione	NOUN
mkg-58282	27	21	s	s	NOUN
mkg-58282	27	22	-	-	PUNCT
mkg-58282	27	23	transferase.11,12	transferase.11,12	NOUN
mkg-58282	27	24	furthermore	furthermore	ADV
mkg-58282	27	25	,	,	PUNCT
mkg-58282	27	26	diluting	dilute	VERB
mkg-58282	27	27	protein	protein	NOUN
mkg-58282	27	28	solutions	solution	NOUN
mkg-58282	27	29	has	have	AUX
mkg-58282	27	30	been	be	AUX
mkg-58282	27	31	shown	show	VERB
mkg-58282	27	32	to	to	PART
mkg-58282	27	33	enhance	enhance	VERB
mkg-58282	27	34	the	the	DET
mkg-58282	27	35	recovery	recovery	NOUN
mkg-58282	27	36	of	of	ADP
mkg-58282	27	37	soluble	soluble	ADJ
mkg-58282	27	38	and	and	CCONJ
mkg-58282	27	39	active	active	ADJ
mkg-58282	27	40	enzymes.13	enzymes.13	NOUN
mkg-58282	27	41	in	in	ADP
mkg-58282	27	42	this	this	DET
mkg-58282	27	43	study	study	NOUN
mkg-58282	27	44	,	,	PUNCT
mkg-58282	27	45	we	we	PRON
mkg-58282	27	46	examined	examine	VERB
mkg-58282	27	47	a	a	DET
mkg-58282	27	48	dilution	dilution	NOUN
mkg-58282	27	49	method	method	NOUN
mkg-58282	27	50	in	in	ADP
mkg-58282	27	51	which	which	PRON
mkg-58282	27	52	proteins	protein	NOUN
mkg-58282	27	53	were	be	AUX
mkg-58282	27	54	expressed	express	VERB
mkg-58282	27	55	in	in	ADP
mkg-58282	27	56	bacteria	bacteria	NOUN
mkg-58282	27	57	,	,	PUNCT
mkg-58282	27	58	purified	purified	ADJ
mkg-58282	27	59	using	use	VERB
mkg-58282	27	60	column	column	NOUN
mkg-58282	27	61	chromatography	chromatography	NOUN
mkg-58282	27	62	and	and	CCONJ
mkg-58282	27	63	diluted	dilute	VERB
mkg-58282	27	64	100	100	NUM
mkg-58282	27	65	-	-	NUM
mkg-58282	27	66	fold	fold	ADJ
mkg-58282	27	67	.	.	PUNCT
mkg-58282	28	1	we	we	PRON
mkg-58282	28	2	hypothesized	hypothesize	VERB
mkg-58282	28	3	that	that	SCONJ
mkg-58282	28	4	chimeric	chimeric	ADJ
mkg-58282	28	5	proteins	protein	NOUN
mkg-58282	28	6	would	would	AUX
mkg-58282	28	7	have	have	VERB
mkg-58282	28	8	a	a	DET
mkg-58282	28	9	lower	low	ADJ
mkg-58282	28	10	propensity	propensity	NOUN
mkg-58282	28	11	to	to	PART
mkg-58282	28	12	aggregate	aggregate	VERB
mkg-58282	28	13	in	in	ADP
mkg-58282	28	14	dilute	dilute	ADJ
mkg-58282	28	15	solutions	solution	NOUN
mkg-58282	28	16	,	,	PUNCT
mkg-58282	28	17	resulting	result	VERB
mkg-58282	28	18	in	in	ADP
mkg-58282	28	19	a	a	DET
mkg-58282	28	20	higher	high	ADJ
mkg-58282	28	21	yield	yield	NOUN
mkg-58282	28	22	of	of	ADP
mkg-58282	28	23	the	the	DET
mkg-58282	28	24	active	active	ADJ
mkg-58282	28	25	form	form	NOUN
mkg-58282	28	26	.	.	PUNCT
mkg-58282	29	1	to	to	PART
mkg-58282	29	2	verify	verify	VERB
mkg-58282	29	3	this	this	DET
mkg-58282	29	4	hypothesis	hypothesis	NOUN
mkg-58282	29	5	,	,	PUNCT
mkg-58282	29	6	we	we	PRON
mkg-58282	29	7	prepared	prepare	VERB
mkg-58282	29	8	a	a	DET
mkg-58282	29	9	recombinant	recombinant	ADJ
mkg-58282	29	10	chimeric	chimeric	ADJ
mkg-58282	29	11	protein	protein	NOUN
mkg-58282	29	12	–	–	PUNCT
mkg-58282	29	13	basic	basic	ADJ
mkg-58282	29	14	fibroblast	fibroblast	ADJ
mkg-58282	29	15	growth	growth	NOUN
mkg-58282	29	16	factor	factor	NOUN
mkg-58282	29	17	fused	fuse	VERB
mkg-58282	29	18	with	with	ADP
mkg-58282	29	19	a	a	DET
mkg-58282	29	20	pentadeca	pentadeca	NOUN
mkg-58282	29	21	peptide	peptide	NOUN
mkg-58282	29	22	as	as	ADP
mkg-58282	29	23	a	a	DET
mkg-58282	29	24	linker	linker	NOUN
mkg-58282	29	25	and	and	CCONJ
mkg-58282	29	26	hexahistidine	hexahistidine	NOUN
mkg-58282	29	27	as	as	ADP
mkg-58282	29	28	an	an	DET
mkg-58282	29	29	affinity	affinity	NOUN
mkg-58282	29	30	tag	tag	NOUN
mkg-58282	29	31	(	(	PUNCT
mkg-58282	29	32	hereafter	hereafter	ADV
mkg-58282	29	33	referred	refer	VERB
mkg-58282	29	34	to	to	ADP
mkg-58282	29	35	as	as	ADP
mkg-58282	29	36	bfgf	bfgf	NOUN
mkg-58282	29	37	-	-	PUNCT
mkg-58282	29	38	ph	ph	ADJ
mkg-58282	29	39	)	)	PUNCT
mkg-58282	29	40	.	.	PUNCT
mkg-58282	30	1	to	to	PART
mkg-58282	30	2	assess	assess	VERB
mkg-58282	30	3	the	the	DET
mkg-58282	30	4	correct	correct	ADJ
mkg-58282	30	5	folding	folding	NOUN
mkg-58282	30	6	of	of	ADP
mkg-58282	30	7	the	the	DET
mkg-58282	30	8	bfgf	bfgf	NOUN
mkg-58282	30	9	domain	domain	NOUN
mkg-58282	30	10	,	,	PUNCT
mkg-58282	30	11	the	the	DET
mkg-58282	30	12	prepared	prepared	ADJ
mkg-58282	30	13	bfgf	bfgf	NOUN
mkg-58282	30	14	-	-	PUNCT
mkg-58282	30	15	ph	ph	NOUN
mkg-58282	30	16	was	be	AUX
mkg-58282	30	17	analyzed	analyze	VERB
mkg-58282	30	18	using	use	VERB
mkg-58282	30	19	circular	circular	ADJ
mkg-58282	30	20	dichroism	dichroism	NOUN
mkg-58282	30	21	(	(	PUNCT
mkg-58282	30	22	cd	cd	NOUN
mkg-58282	30	23	)	)	PUNCT
mkg-58282	30	24	spectroscopy	spectroscopy	NOUN
mkg-58282	30	25	,	,	PUNCT
mkg-58282	30	26	and	and	CCONJ
mkg-58282	30	27	the	the	DET
mkg-58282	30	28	resulting	result	VERB
mkg-58282	30	29	spectrum	spectrum	NOUN
mkg-58282	30	30	was	be	AUX
mkg-58282	30	31	compared	compare	VERB
mkg-58282	30	32	with	with	ADP
mkg-58282	30	33	a	a	DET
mkg-58282	30	34	computationally	computationally	ADV
mkg-58282	30	35	predicted	predict	VERB
mkg-58282	30	36	reference	reference	NOUN
mkg-58282	30	37	spectrum	spectrum	NOUN
mkg-58282	30	38	.	.	PUNCT
mkg-58282	31	1	materials	material	NOUN
mkg-58282	31	2	and	and	CCONJ
mkg-58282	31	3	methods	method	NOUN
mkg-58282	31	4	figure	figure	VERB
mkg-58282	31	5	1	1	NUM
mkg-58282	31	6	shows	show	VERB
mkg-58282	31	7	the	the	DET
mkg-58282	31	8	amino	amino	NOUN
mkg-58282	31	9	acid	acid	NOUN
mkg-58282	31	10	sequence	sequence	NOUN
mkg-58282	31	11	of	of	ADP
mkg-58282	31	12	the	the	DET
mkg-58282	31	13	engineered	engineer	VERB
mkg-58282	31	14	bfgf	bfgf	NOUN
mkg-58282	31	15	prepared	prepare	VERB
mkg-58282	31	16	in	in	ADP
mkg-58282	31	17	this	this	DET
mkg-58282	31	18	study	study	NOUN
mkg-58282	31	19	.	.	PUNCT
mkg-58282	32	1	at	at	ADP
mkg-58282	32	2	the	the	DET
mkg-58282	32	3	c	c	NOUN
mkg-58282	32	4	-	-	PUNCT
mkg-58282	32	5	terminus	terminus	NOUN
mkg-58282	32	6	of	of	ADP
mkg-58282	32	7	bfgf	bfgf	NOUN
mkg-58282	32	8	,	,	PUNCT
mkg-58282	32	9	pentadeca	pentadeca	NOUN
mkg-58282	32	10	(	(	PUNCT
mkg-58282	32	11	ggggs)3	ggggs)3	NUM
mkg-58282	32	12	and	and	CCONJ
mkg-58282	32	13	hexahistidine	hexahistidine	NOUN
mkg-58282	32	14	peptides	peptide	NOUN
mkg-58282	32	15	were	be	AUX
mkg-58282	32	16	added	add	VERB
mkg-58282	32	17	as	as	ADP
mkg-58282	32	18	a	a	DET
mkg-58282	32	19	linker	linker	NOUN
mkg-58282	32	20	and	and	CCONJ
mkg-58282	32	21	an	an	DET
mkg-58282	32	22	affinity	affinity	NOUN
mkg-58282	32	23	tag	tag	NOUN
mkg-58282	32	24	,	,	PUNCT
mkg-58282	32	25	respectively	respectively	ADV
mkg-58282	32	26	.	.	PUNCT
mkg-58282	33	1	we	we	PRON
mkg-58282	33	2	expected	expect	VERB
mkg-58282	33	3	that	that	SCONJ
mkg-58282	33	4	the	the	DET
mkg-58282	33	5	nonionic	nonionic	ADJ
mkg-58282	33	6	glycine	glycine	NOUN
mkg-58282	33	7	(	(	PUNCT
mkg-58282	33	8	g	g	NOUN
mkg-58282	33	9	)	)	PUNCT
mkg-58282	33	10	peptide	peptide	NOUN
mkg-58282	33	11	would	would	AUX
mkg-58282	33	12	help	help	VERB
mkg-58282	33	13	maintain	maintain	VERB
mkg-58282	33	14	the	the	DET
mkg-58282	33	15	distance	distance	NOUN
mkg-58282	33	16	between	between	ADP
mkg-58282	33	17	the	the	DET
mkg-58282	33	18	two	two	NUM
mkg-58282	33	19	segments	segment	NOUN
mkg-58282	33	20	while	while	SCONJ
mkg-58282	33	21	serine	serine	NOUN
mkg-58282	33	22	(	(	PUNCT
mkg-58282	33	23	s	s	X
mkg-58282	33	24	)	)	PUNCT
mkg-58282	33	25	would	would	AUX
mkg-58282	33	26	provide	provide	VERB
mkg-58282	33	27	flexibility	flexibility	NOUN
mkg-58282	33	28	to	to	ADP
mkg-58282	33	29	the	the	DET
mkg-58282	33	30	linker	linker	NOUN
mkg-58282	33	31	.	.	PUNCT
mkg-58282	33	32	to	to	PART
mkg-58282	33	33	express	express	VERB
mkg-58282	33	34	these	these	DET
mkg-58282	33	35	proteins	protein	NOUN
mkg-58282	33	36	in	in	ADP
mkg-58282	33	37	bacteria	bacteria	NOUN
mkg-58282	33	38	,	,	PUNCT
mkg-58282	33	39	a	a	DET
mkg-58282	33	40	dna	dna	PROPN
mkg-58282	33	41	insert	insert	NOUN
mkg-58282	33	42	encoding	encode	VERB
mkg-58282	33	43	bfgf	bfgf	NOUN
mkg-58282	33	44	-	-	PUNCT
mkg-58282	33	45	ph	ph	NOUN
mkg-58282	33	46	was	be	AUX
mkg-58282	33	47	synthesized	synthesize	VERB
mkg-58282	33	48	using	use	VERB
mkg-58282	33	49	the	the	DET
mkg-58282	33	50	geneart	geneart	NOUN
mkg-58282	33	51	strings	string	NOUN
mkg-58282	33	52	dna	dna	PROPN
mkg-58282	33	53	fragments	fragment	VERB
mkg-58282	33	54	service	service	NOUN
mkg-58282	33	55	(	(	PUNCT
mkg-58282	33	56	thermo	thermo	NOUN
mkg-58282	33	57	fisher	fisher	PROPN
mkg-58282	33	58	scientific	scientific	PROPN
mkg-58282	33	59	,	,	PUNCT
mkg-58282	33	60	waltham	waltham	PROPN
mkg-58282	33	61	,	,	PUNCT
mkg-58282	33	62	ma	ma	PROPN
mkg-58282	33	63	,	,	PUNCT
mkg-58282	33	64	usa	usa	PROPN
mkg-58282	33	65	)	)	PUNCT
mkg-58282	33	66	.	.	PUNCT
mkg-58282	34	1	the	the	DET
mkg-58282	34	2	obtained	obtain	VERB
mkg-58282	34	3	dna	dna	PROPN
mkg-58282	34	4	fragment	fragment	NOUN
mkg-58282	34	5	was	be	AUX
mkg-58282	34	6	inserted	insert	VERB
mkg-58282	34	7	into	into	ADP
mkg-58282	34	8	the	the	DET
mkg-58282	34	9	multiple	multiple	ADJ
mkg-58282	34	10	cloning	cloning	NOUN
mkg-58282	34	11	sites	site	NOUN
mkg-58282	34	12	of	of	ADP
mkg-58282	34	13	pet-22b(+	pet-22b(+	PROPN
mkg-58282	34	14	)	)	PUNCT
mkg-58282	34	15	(	(	PUNCT
mkg-58282	34	16	69744	69744	NUM
mkg-58282	34	17	,	,	PUNCT
mkg-58282	34	18	novagen	novagen	PROPN
mkg-58282	34	19	,	,	PUNCT
mkg-58282	34	20	madison	madison	PROPN
mkg-58282	34	21	,	,	PUNCT
mkg-58282	34	22	wi	wi	PROPN
mkg-58282	34	23	,	,	PUNCT
mkg-58282	34	24	usa	usa	PROPN
mkg-58282	34	25	)	)	PUNCT
mkg-58282	34	26	using	use	VERB
mkg-58282	34	27	the	the	DET
mkg-58282	34	28	seamless	seamless	ADJ
mkg-58282	34	29	cloning	cloning	NOUN
mkg-58282	34	30	method,14	method,14	ADV
mkg-58282	34	31	employing	employ	VERB
mkg-58282	34	32	an	an	DET
mkg-58282	34	33	in	in	ADP
mkg-58282	34	34	-	-	PUNCT
mkg-58282	34	35	fusion	fusion	NOUN
mkg-58282	34	36	hd	hd	NOUN
mkg-58282	34	37	cloning	cloning	NOUN
mkg-58282	34	38	kit	kit	NOUN
mkg-58282	34	39	(	(	PUNCT
mkg-58282	34	40	z9648n	z9648n	NOUN
mkg-58282	34	41	,	,	PUNCT
mkg-58282	34	42	clontech	clontech	NOUN
mkg-58282	34	43	laboratories	laboratory	NOUN
mkg-58282	34	44	,	,	PUNCT
mkg-58282	34	45	inc	inc	PROPN
mkg-58282	34	46	.	.	PROPN
mkg-58282	34	47	,	,	PUNCT
mkg-58282	34	48	mountain	mountain	NOUN
mkg-58282	34	49	view	view	NOUN
mkg-58282	34	50	,	,	PUNCT
mkg-58282	34	51	ca	ca	PROPN
mkg-58282	34	52	,	,	PUNCT
mkg-58282	34	53	usa	usa	PROPN
mkg-58282	34	54	)	)	PUNCT
mkg-58282	34	55	.	.	PUNCT
mkg-58282	35	1	sequence	sequence	NOUN
mkg-58282	35	2	accuracy	accuracy	NOUN
mkg-58282	35	3	was	be	AUX
mkg-58282	35	4	confirmed	confirm	VERB
mkg-58282	35	5	by	by	ADP
mkg-58282	35	6	sequencing	sequence	VERB
mkg-58282	35	7	.	.	PUNCT
mkg-58282	36	1	the	the	DET
mkg-58282	36	2	plasmid	plasmid	NOUN
mkg-58282	36	3	harboring	harbor	VERB
mkg-58282	36	4	the	the	DET
mkg-58282	36	5	bfgf	bfgf	ADJ
mkg-58282	36	6	-	-	PUNCT
mkg-58282	36	7	ph	ph	ADJ
mkg-58282	36	8	gene	gene	NOUN
mkg-58282	36	9	was	be	AUX
mkg-58282	36	10	transferred	transfer	VERB
mkg-58282	36	11	to	to	ADP
mkg-58282	36	12	escherichia	escherichia	NOUN
mkg-58282	36	13	coli	coli	NOUN
mkg-58282	36	14	strain	strain	NOUN
mkg-58282	36	15	bl21	bl21	PROPN
mkg-58282	36	16	-	-	PUNCT
mkg-58282	36	17	codonplus	codonplus	NOUN
mkg-58282	36	18	(	(	PUNCT
mkg-58282	36	19	de3)-ripl	de3)-ripl	X
mkg-58282	36	20	(	(	PUNCT
mkg-58282	36	21	230280	230280	NUM
mkg-58282	36	22	,	,	PUNCT
mkg-58282	36	23	agilent	agilent	PROPN
mkg-58282	36	24	technologies	technologies	PROPN
mkg-58282	36	25	,	,	PUNCT
mkg-58282	36	26	inc	inc	PROPN
mkg-58282	36	27	.	.	PROPN
mkg-58282	36	28	,	,	PUNCT
mkg-58282	36	29	santa	santa	PROPN
mkg-58282	36	30	clara	clara	PROPN
mkg-58282	36	31	,	,	PUNCT
mkg-58282	36	32	ca	ca	PROPN
mkg-58282	36	33	,	,	PUNCT
mkg-58282	36	34	usa	usa	PROPN
mkg-58282	36	35	)	)	PUNCT
mkg-58282	36	36	and	and	CCONJ
mkg-58282	36	37	cultured	cultured	ADJ
mkg-58282	36	38	in	in	ADP
mkg-58282	36	39	200	200	NUM
mkg-58282	36	40	ml	ml	NOUN
mkg-58282	36	41	lysogeny	lysogeny	NOUN
mkg-58282	36	42	broth	broth	NOUN
mkg-58282	36	43	(	(	PUNCT
mkg-58282	36	44	lb	lb	NOUN
mkg-58282	36	45	)	)	PUNCT
mkg-58282	36	46	medium	medium	NOUN
mkg-58282	36	47	with	with	ADP
mkg-58282	36	48	100	100	NUM
mkg-58282	36	49	µg	µg	ADJ
mkg-58282	36	50	/	/	SYM
mkg-58282	36	51	ml	ml	NOUN
mkg-58282	36	52	ampicillin	ampicillin	NOUN
mkg-58282	36	53	(	(	PUNCT
mkg-58282	36	54	02739	02739	NUM
mkg-58282	36	55	-	-	SYM
mkg-58282	36	56	32	32	NUM
mkg-58282	36	57	,	,	PUNCT
mkg-58282	36	58	nacalai	nacalai	NOUN
mkg-58282	36	59	tesque	tesque	PROPN
mkg-58282	36	60	,	,	PUNCT
mkg-58282	36	61	kyoto	kyoto	PROPN
mkg-58282	36	62	,	,	PUNCT
mkg-58282	36	63	japan	japan	PROPN
mkg-58282	36	64	)	)	PUNCT
mkg-58282	36	65	at	at	ADP
mkg-58282	36	66	37	37	NUM
mkg-58282	36	67	°	°	NOUN
mkg-58282	36	68	c	c	NOUN
mkg-58282	36	69	.	.	PUNCT
mkg-58282	37	1	when	when	SCONJ
mkg-58282	37	2	the	the	DET
mkg-58282	37	3	medium	medium	ADJ
mkg-58282	37	4	turbidity	turbidity	NOUN
mkg-58282	37	5	reached	reach	VERB
mkg-58282	37	6	0.4	0.4	NUM
mkg-58282	37	7	–	–	PUNCT
mkg-58282	37	8	0.8	0.8	NUM
mkg-58282	37	9	,	,	PUNCT
mkg-58282	37	10	1	1	NUM
mkg-58282	37	11	mm	mm	NOUN
mkg-58282	37	12	isopropyl	isopropyl	NOUN
mkg-58282	37	13	β	β	NOUN
mkg-58282	37	14	-	-	PUNCT
mkg-58282	37	15	d	d	NOUN
mkg-58282	37	16	-	-	NOUN
mkg-58282	37	17	thiogalactopyranoside	thiogalactopyranoside	NOUN
mkg-58282	37	18	(	(	PUNCT
mkg-58282	37	19	06289	06289	NUM
mkg-58282	37	20	-	-	SYM
mkg-58282	37	21	67	67	NUM
mkg-58282	37	22	,	,	PUNCT
mkg-58282	37	23	nacalai	nacalai	PROPN
mkg-58282	37	24	tesque	tesque	NOUN
mkg-58282	37	25	)	)	PUNCT
mkg-58282	37	26	was	be	AUX
mkg-58282	37	27	added	add	VERB
mkg-58282	37	28	to	to	PART
mkg-58282	37	29	induce	induce	VERB
mkg-58282	37	30	protein	protein	NOUN
mkg-58282	37	31	expression	expression	NOUN
mkg-58282	37	32	,	,	PUNCT
mkg-58282	37	33	followed	follow	VERB
mkg-58282	37	34	by	by	ADP
mkg-58282	37	35	incubation	incubation	NOUN
mkg-58282	37	36	at	at	ADP
mkg-58282	37	37	37	37	NUM
mkg-58282	37	38	°	°	ADP
mkg-58282	37	39	c	c	NOUN
mkg-58282	37	40	for	for	ADP
mkg-58282	37	41	an	an	DET
mkg-58282	37	42	additional	additional	ADJ
mkg-58282	37	43	3	3	NUM
mkg-58282	37	44	hours	hour	NOUN
mkg-58282	37	45	.	.	PUNCT
mkg-58282	38	1	the	the	DET
mkg-58282	38	2	cells	cell	NOUN
mkg-58282	38	3	were	be	AUX
mkg-58282	38	4	lysed	lyse	VERB
mkg-58282	38	5	using	use	VERB
mkg-58282	38	6	bugbuster	bugbuster	ADJ
mkg-58282	38	7	reagent	reagent	NOUN
mkg-58282	38	8	(	(	PUNCT
mkg-58282	38	9	70584	70584	NUM
mkg-58282	38	10	-	-	SYM
mkg-58282	38	11	4	4	NUM
mkg-58282	38	12	,	,	PUNCT
mkg-58282	38	13	emd	emd	PROPN
mkg-58282	38	14	millipore	millipore	PROPN
mkg-58282	38	15	corp	corp	PROPN
mkg-58282	38	16	.	.	PROPN
mkg-58282	38	17	,	,	PUNCT
mkg-58282	38	18	burlington	burlington	PROPN
mkg-58282	38	19	,	,	PUNCT
mkg-58282	38	20	ma	ma	PROPN
mkg-58282	38	21	,	,	PUNCT
mkg-58282	38	22	usa	usa	PROPN
mkg-58282	38	23	)	)	PUNCT
mkg-58282	38	24	containing	contain	VERB
mkg-58282	38	25	10	10	NUM
mkg-58282	38	26	µg	µg	ADJ
mkg-58282	38	27	/	/	SYM
mkg-58282	38	28	ml	ml	NOUN
mkg-58282	38	29	lysozyme	lysozyme	NOUN
mkg-58282	38	30	(	(	PUNCT
mkg-58282	38	31	l6876	l6876	NOUN
mkg-58282	38	32	,	,	PUNCT
mkg-58282	38	33	sigma	sigma	PROPN
mkg-58282	38	34	-	-	PUNCT
mkg-58282	38	35	aldrich	aldrich	PROPN
mkg-58282	38	36	,	,	PUNCT
mkg-58282	38	37	st	st	PROPN
mkg-58282	38	38	.	.	PROPN
mkg-58282	38	39	louis	louis	PROPN
mkg-58282	38	40	,	,	PUNCT
mkg-58282	38	41	mo	mo	PROPN
mkg-58282	38	42	,	,	PUNCT
mkg-58282	38	43	usa	usa	PROPN
mkg-58282	38	44	)	)	PUNCT
mkg-58282	38	45	and	and	CCONJ
mkg-58282	38	46	10	10	NUM
mkg-58282	38	47	u	u	NOUN
mkg-58282	38	48	/	/	SYM
mkg-58282	38	49	ml	ml	NOUN
mkg-58282	38	50	benzonase	benzonase	NOUN
mkg-58282	38	51	(	(	PUNCT
mkg-58282	38	52	70746	70746	NUM
mkg-58282	38	53	-	-	SYM
mkg-58282	38	54	3	3	NUM
mkg-58282	38	55	,	,	PUNCT
mkg-58282	38	56	novagen	novagen	NOUN
mkg-58282	38	57	)	)	PUNCT
mkg-58282	38	58	.	.	PUNCT
mkg-58282	39	1	subsequently	subsequently	ADV
mkg-58282	39	2	,	,	PUNCT
mkg-58282	39	3	bfgf	bfgf	NOUN
mkg-58282	39	4	-	-	PUNCT
mkg-58282	39	5	ph	ph	NOUN
mkg-58282	39	6	in	in	ADP
mkg-58282	39	7	the	the	DET
mkg-58282	39	8	soluble	soluble	ADJ
mkg-58282	39	9	fraction	fraction	NOUN
mkg-58282	39	10	was	be	AUX
mkg-58282	39	11	purified	purify	VERB
mkg-58282	39	12	by	by	ADP
mkg-58282	39	13	metal	metal	NOUN
mkg-58282	39	14	chelate	chelate	NOUN
mkg-58282	39	15	affinity	affinity	NOUN
mkg-58282	39	16	chromatography	chromatography	NOUN
mkg-58282	39	17	using	use	VERB
mkg-58282	39	18	a	a	DET
mkg-58282	39	19	histrap	histrap	NOUN
mkg-58282	39	20	hp	hp	PROPN
mkg-58282	39	21	column	column	NOUN
mkg-58282	39	22	(	(	PUNCT
mkg-58282	39	23	17524801	17524801	NUM
mkg-58282	39	24	,	,	PUNCT
mkg-58282	39	25	cytiva	cytiva	PROPN
mkg-58282	39	26	,	,	PUNCT
mkg-58282	39	27	chicago	chicago	PROPN
mkg-58282	39	28	,	,	PUNCT
mkg-58282	39	29	il	il	PROPN
mkg-58282	39	30	,	,	PUNCT
mkg-58282	39	31	usa	usa	PROPN
mkg-58282	39	32	)	)	PUNCT
mkg-58282	39	33	.	.	PUNCT
mkg-58282	40	1	the	the	DET
mkg-58282	40	2	protein	protein	NOUN
mkg-58282	40	3	solution	solution	NOUN
mkg-58282	40	4	was	be	AUX
mkg-58282	40	5	dialyzed	dialyze	VERB
mkg-58282	40	6	against	against	ADP
mkg-58282	40	7	20	20	NUM
mkg-58282	40	8	mm	mm	NOUN
mkg-58282	40	9	phosphate	phosphate	NOUN
mkg-58282	40	10	buffer	buffer	NOUN
mkg-58282	40	11	(	(	PUNCT
mkg-58282	40	12	pb	pb	X
mkg-58282	40	13	)	)	PUNCT
mkg-58282	40	14	containing	contain	VERB
mkg-58282	40	15	500	500	NUM
mkg-58282	40	16	mm	mm	NOUN
mkg-58282	40	17	nacl	nacl	NOUN
mkg-58282	40	18	(	(	PUNCT
mkg-58282	40	19	ph	ph	NOUN
mkg-58282	40	20	7.4	7.4	NUM
mkg-58282	40	21	;	;	PUNCT
mkg-58282	40	22	191	191	NUM
mkg-58282	40	23	-	-	SYM
mkg-58282	40	24	01665	01665	NUM
mkg-58282	40	25	,	,	PUNCT
mkg-58282	40	26	fujifilm	fujifilm	PROPN
mkg-58282	40	27	wako	wako	PROPN
mkg-58282	40	28	pure	pure	PROPN
mkg-58282	40	29	chemical	chemical	PROPN
mkg-58282	40	30	corp	corp	PROPN
mkg-58282	40	31	.	.	PROPN
mkg-58282	40	32	,	,	PUNCT
mkg-58282	40	33	osaka	osaka	PROPN
mkg-58282	40	34	,	,	PUNCT
mkg-58282	40	35	japan	japan	PROPN
mkg-58282	40	36	)	)	PUNCT
mkg-58282	40	37	,	,	PUNCT
mkg-58282	40	38	followed	follow	VERB
mkg-58282	40	39	by	by	ADP
mkg-58282	40	40	dialysis	dialysis	NOUN
mkg-58282	40	41	in	in	ADP
mkg-58282	40	42	20	20	NUM
mkg-58282	40	43	mm	mm	NOUN
mkg-58282	40	44	citrate	citrate	NOUN
mkg-58282	40	45	buffer	buffer	NOUN
mkg-58282	40	46	(	(	PUNCT
mkg-58282	40	47	ph	ph	NOUN
mkg-58282	40	48	5.0),6,15	5.0),6,15	NUM
mkg-58282	40	49	with	with	ADP
mkg-58282	40	50	or	or	CCONJ
mkg-58282	40	51	without	without	ADP
mkg-58282	40	52	500	500	NUM
mkg-58282	40	53	mm	mm	NOUN
mkg-58282	40	54	nacl	nacl	NOUN
mkg-58282	40	55	.	.	PUNCT
mkg-58282	41	1	this	this	PRON
mkg-58282	41	2	was	be	AUX
mkg-58282	41	3	followed	follow	VERB
mkg-58282	41	4	by	by	ADP
mkg-58282	41	5	another	another	DET
mkg-58282	41	6	round	round	NOUN
mkg-58282	41	7	of	of	ADP
mkg-58282	41	8	dialysis	dialysis	NOUN
mkg-58282	41	9	in	in	ADP
mkg-58282	41	10	20	20	NUM
mkg-58282	41	11	mm	mm	NOUN
mkg-58282	41	12	pb	pb	ADP
mkg-58282	41	13	containing	contain	VERB
mkg-58282	41	14	500	500	NUM
mkg-58282	41	15	mm	mm	NOUN
mkg-58282	41	16	nacl	nacl	NOUN
mkg-58282	41	17	(	(	PUNCT
mkg-58282	41	18	ph	ph	NOUN
mkg-58282	41	19	7.4	7.4	NUM
mkg-58282	41	20	)	)	PUNCT
mkg-58282	41	21	.	.	PUNCT
mkg-58282	42	1	alternatively	alternatively	ADV
mkg-58282	42	2	,	,	PUNCT
mkg-58282	42	3	a	a	DET
mkg-58282	42	4	bfgf	bfgf	ADJ
mkg-58282	42	5	-	-	PUNCT
mkg-58282	42	6	ph	ph	ADJ
mkg-58282	42	7	solution	solution	NOUN
mkg-58282	42	8	obtained	obtain	VERB
mkg-58282	42	9	immediately	immediately	ADV
mkg-58282	42	10	after	after	SCONJ
mkg-58282	42	11	affinity	affinity	NOUN
mkg-58282	42	12	purification	purification	NOUN
mkg-58282	42	13	was	be	AUX
mkg-58282	42	14	diluted	dilute	VERB
mkg-58282	42	15	100	100	NUM
mkg-58282	42	16	-	-	NUM
mkg-58282	42	17	fold	fold	VERB
mkg-58282	42	18	with	with	ADP
mkg-58282	42	19	20	20	NUM
mkg-58282	42	20	mm	mm	NOUN
mkg-58282	42	21	citrate	citrate	NOUN
mkg-58282	42	22	buffer	buffer	NOUN
mkg-58282	42	23	(	(	PUNCT
mkg-58282	42	24	ph	ph	NOUN
mkg-58282	42	25	5.0	5.0	NUM
mkg-58282	42	26	)	)	PUNCT
mkg-58282	42	27	to	to	PART
mkg-58282	42	28	initiate	initiate	VERB
mkg-58282	42	29	protein	protein	NOUN
mkg-58282	42	30	refolding	refolding	NOUN
mkg-58282	42	31	.	.	PUNCT
mkg-58282	43	1	then	then	ADV
mkg-58282	43	2	,	,	PUNCT
mkg-58282	43	3	500	500	NUM
mkg-58282	43	4	mm	mm	PROPN
mkg-58282	43	5	nacl	nacl	NOUN
mkg-58282	43	6	was	be	AUX
mkg-58282	43	7	added	add	VERB
mkg-58282	43	8	to	to	ADP
mkg-58282	43	9	the	the	DET
mkg-58282	43	10	solution	solution	NOUN
mkg-58282	43	11	.	.	PUNCT
mkg-58282	44	1	the	the	DET
mkg-58282	44	2	protein	protein	NOUN
mkg-58282	44	3	was	be	AUX
mkg-58282	44	4	subsequently	subsequently	ADV
mkg-58282	44	5	concentrated	concentrate	VERB
mkg-58282	44	6	using	use	VERB
mkg-58282	44	7	a	a	DET
mkg-58282	44	8	histrap	histrap	NOUN
mkg-58282	44	9	hp	hp	PROPN
mkg-58282	44	10	column	column	NOUN
mkg-58282	44	11	,	,	PUNCT
mkg-58282	44	12	yielding	yield	VERB
mkg-58282	44	13	a	a	DET
mkg-58282	44	14	final	final	ADJ
mkg-58282	44	15	bfgf	bfgf	NOUN
mkg-58282	44	16	-	-	PUNCT
mkg-58282	44	17	ph	ph	ADJ
mkg-58282	44	18	solution	solution	NOUN
mkg-58282	44	19	in	in	ADP
mkg-58282	44	20	20	20	NUM
mkg-58282	44	21	mm	mm	NOUN
mkg-58282	44	22	pb	pb	ADP
mkg-58282	44	23	containing	contain	VERB
mkg-58282	44	24	500	500	NUM
mkg-58282	44	25	mm	mm	NOUN
mkg-58282	44	26	nacl	nacl	NOUN
mkg-58282	44	27	(	(	PUNCT
mkg-58282	44	28	ph	ph	NOUN
mkg-58282	44	29	7.4	7.4	NUM
mkg-58282	44	30	)	)	PUNCT
mkg-58282	44	31	.	.	PUNCT
mkg-58282	45	1	the	the	DET
mkg-58282	45	2	concentration	concentration	NOUN
mkg-58282	45	3	of	of	ADP
mkg-58282	45	4	bfgf	bfgf	NOUN
mkg-58282	45	5	-	-	PUNCT
mkg-58282	45	6	ph	ph	NOUN
mkg-58282	45	7	was	be	AUX
mkg-58282	45	8	determined	determine	VERB
mkg-58282	45	9	using	use	VERB
mkg-58282	45	10	a	a	DET
mkg-58282	45	11	micro	micro	ADJ
mkg-58282	45	12	bca	bca	PROPN
mkg-58282	45	13	protein	protein	NOUN
mkg-58282	45	14	assay	assay	NOUN
mkg-58282	45	15	kit	kit	NOUN
mkg-58282	45	16	(	(	PUNCT
mkg-58282	45	17	23235	23235	NUM
mkg-58282	45	18	,	,	PUNCT
mkg-58282	45	19	thermo	thermo	NOUN
mkg-58282	45	20	fisher	fisher	PROPN
mkg-58282	45	21	scientific	scientific	PROPN
mkg-58282	45	22	inc	inc	PROPN
mkg-58282	45	23	.	.	PROPN
mkg-58282	45	24	,	,	PUNCT
mkg-58282	45	25	waltham	waltham	PROPN
mkg-58282	45	26	,	,	PUNCT
mkg-58282	45	27	ma	ma	PROPN
mkg-58282	45	28	,	,	PUNCT
mkg-58282	45	29	usa	usa	PROPN
mkg-58282	45	30	)	)	PUNCT
mkg-58282	45	31	.	.	PUNCT
mkg-58282	46	1	purity	purity	NOUN
mkg-58282	46	2	and	and	CCONJ
mkg-58282	46	3	molecular	molecular	ADJ
mkg-58282	46	4	size	size	NOUN
mkg-58282	46	5	were	be	AUX
mkg-58282	46	6	analyzed	analyze	VERB
mkg-58282	46	7	by	by	ADP
mkg-58282	46	8	sodium	sodium	NOUN
mkg-58282	46	9	dodecyl	dodecyl	PROPN
mkg-58282	46	10	sulfate	sulfate	NOUN
mkg-58282	46	11	-	-	PUNCT
mkg-58282	46	12	polyacrylamide	polyacrylamide	NOUN
mkg-58282	46	13	gel	gel	NOUN
mkg-58282	46	14	electrophoresis	electrophoresis	NOUN
mkg-58282	46	15	(	(	PUNCT
mkg-58282	46	16	sds	sds	NOUN
mkg-58282	46	17	-	-	PUNCT
mkg-58282	46	18	page	page	NOUN
mkg-58282	46	19	)	)	PUNCT
mkg-58282	46	20	with	with	ADP
mkg-58282	46	21	coomassie	coomassie	ADJ
mkg-58282	46	22	brilliant	brilliant	ADJ
mkg-58282	46	23	blue	blue	ADJ
mkg-58282	46	24	staining	staining	NOUN
mkg-58282	46	25	.	.	PUNCT
mkg-58282	47	1	all	all	DET
mkg-58282	47	2	recombinant	recombinant	ADJ
mkg-58282	47	3	dna	dna	NOUN
mkg-58282	47	4	experiments	experiment	NOUN
mkg-58282	47	5	were	be	AUX
mkg-58282	47	6	conducted	conduct	VERB
mkg-58282	47	7	with	with	ADP
mkg-58282	47	8	the	the	DET
mkg-58282	47	9	approval	approval	NOUN
mkg-58282	47	10	of	of	ADP
mkg-58282	47	11	the	the	DET
mkg-58282	47	12	hiroshima	hiroshima	PROPN
mkg-58282	47	13	university	university	PROPN
mkg-58282	47	14	safety	safety	PROPN
mkg-58282	47	15	committee	committee	PROPN
mkg-58282	47	16	for	for	ADP
mkg-58282	47	17	living	live	VERB
mkg-58282	47	18	modified	modified	ADJ
mkg-58282	47	19	organisms	organism	NOUN
mkg-58282	47	20	.	.	PUNCT
mkg-58282	48	1	the	the	DET
mkg-58282	48	2	secondary	secondary	ADJ
mkg-58282	48	3	structure	structure	NOUN
mkg-58282	48	4	of	of	ADP
mkg-58282	48	5	bfgf	bfgf	NOUN
mkg-58282	48	6	-	-	PUNCT
mkg-58282	48	7	ph	ph	NOUN
mkg-58282	48	8	was	be	AUX
mkg-58282	48	9	analyzed	analyze	VERB
mkg-58282	48	10	by	by	ADP
mkg-58282	48	11	cd	cd	PROPN
mkg-58282	48	12	spectroscopy	spectroscopy	NOUN
mkg-58282	48	13	using	use	VERB
mkg-58282	48	14	a	a	DET
mkg-58282	48	15	jasco	jasco	PROPN
mkg-58282	48	16	j-850	j-850	NOUN
mkg-58282	48	17	spectropolarimeter	spectropolarimeter	NOUN
mkg-58282	48	18	(	(	PUNCT
mkg-58282	48	19	jasco	jasco	PROPN
mkg-58282	48	20	corp	corp	PROPN
mkg-58282	48	21	.	.	PROPN
mkg-58282	48	22	,	,	PUNCT
mkg-58282	48	23	tokyo	tokyo	PROPN
mkg-58282	48	24	,	,	PUNCT
mkg-58282	48	25	japan	japan	PROPN
mkg-58282	48	26	)	)	PUNCT
mkg-58282	48	27	.	.	PUNCT
mkg-58282	49	1	the	the	DET
mkg-58282	49	2	far	far	ADV
mkg-58282	49	3	-	-	PUNCT
mkg-58282	49	4	ultraviolet	ultraviolet	NOUN
mkg-58282	49	5	cd	cd	NOUN
mkg-58282	49	6	spectrum	spectrum	NOUN
mkg-58282	49	7	of	of	ADP
mkg-58282	49	8	bfgf	bfgf	NOUN
mkg-58282	49	9	-	-	PUNCT
mkg-58282	49	10	ph	ph	NOUN
mkg-58282	49	11	was	be	AUX
mkg-58282	49	12	recorded	record	VERB
mkg-58282	49	13	in	in	ADP
mkg-58282	49	14	20	20	NUM
mkg-58282	49	15	mm	mm	NOUN
mkg-58282	49	16	pb	pb	ADP
mkg-58282	49	17	containing	contain	VERB
mkg-58282	49	18	500	500	NUM
mkg-58282	49	19	mm	mm	NOUN
mkg-58282	49	20	nacl	nacl	NOUN
mkg-58282	49	21	(	(	PUNCT
mkg-58282	49	22	ph	ph	NOUN
mkg-58282	49	23	7.4	7.4	NUM
mkg-58282	49	24	)	)	PUNCT
mkg-58282	49	25	at	at	ADP
mkg-58282	49	26	20	20	NUM
mkg-58282	49	27	°	°	NOUN
mkg-58282	49	28	c	c	NOUN
mkg-58282	49	29	.	.	PUNCT
mkg-58282	50	1	to	to	PART
mkg-58282	50	2	determine	determine	VERB
mkg-58282	50	3	whether	whether	SCONJ
mkg-58282	50	4	bfgf	bfgf	NOUN
mkg-58282	50	5	-	-	PUNCT
mkg-58282	50	6	ph	ph	NOUN
mkg-58282	50	7	was	be	AUX
mkg-58282	50	8	correctly	correctly	ADV
mkg-58282	50	9	folded	fold	VERB
mkg-58282	50	10	,	,	PUNCT
mkg-58282	50	11	an	an	DET
mkg-58282	50	12	ideal	ideal	ADJ
mkg-58282	50	13	cd	cd	NOUN
mkg-58282	50	14	spectrum	spectrum	NOUN
mkg-58282	50	15	was	be	AUX
mkg-58282	50	16	generated	generate	VERB
mkg-58282	50	17	using	use	VERB
mkg-58282	50	18	the	the	DET
mkg-58282	50	19	pdbmd2cd	pdbmd2cd	NOUN
mkg-58282	50	20	web	web	NOUN
mkg-58282	50	21	server,16,17	server,16,17	NOUN
mkg-58282	50	22	and	and	CCONJ
mkg-58282	50	23	compared	compare	VERB
mkg-58282	50	24	with	with	ADP
mkg-58282	50	25	the	the	DET
mkg-58282	50	26	experimental	experimental	ADJ
mkg-58282	50	27	spectra	spectra	NOUN
mkg-58282	50	28	.	.	PUNCT
mkg-58282	51	1	the	the	DET
mkg-58282	51	2	server	server	NOUN
mkg-58282	51	3	generated	generate	VERB
mkg-58282	51	4	a	a	DET
mkg-58282	51	5	cd	cd	PROPN
mkg-58282	51	6	spectrum	spectrum	NOUN
mkg-58282	51	7	based	base	VERB
mkg-58282	51	8	on	on	ADP
mkg-58282	51	9	an	an	DET
mkg-58282	51	10	atomic	atomic	ADJ
mkg-58282	51	11	coordinate	coordinate	NOUN
mkg-58282	51	12	file	file	NOUN
mkg-58282	51	13	obtained	obtain	VERB
mkg-58282	51	14	through	through	ADP
mkg-58282	51	15	structural	structural	ADJ
mkg-58282	51	16	prediction	prediction	NOUN
mkg-58282	51	17	using	use	VERB
mkg-58282	51	18	the	the	DET
mkg-58282	51	19	alphafold2	alphafold2	ADJ
mkg-58282	51	20	program,18,19	program,18,19	NOUN
mkg-58282	51	21	an	an	DET
mkg-58282	51	22	open	open	ADJ
mkg-58282	51	23	-	-	PUNCT
mkg-58282	51	24	source	source	NOUN
mkg-58282	51	25	tool	tool	NOUN
mkg-58282	51	26	available	available	ADJ
mkg-58282	51	27	at	at	ADP
mkg-58282	51	28	https://github.com/deepmind/alphafold	https://github.com/deepmind/alphafold	PROPN
mkg-58282	51	29	.	.	PUNCT
mkg-58282	52	1	figure	figure	NOUN
mkg-58282	52	2	1	1	NUM
mkg-58282	52	3	.	.	PUNCT
mkg-58282	52	4	amino	amino	NOUN
mkg-58282	52	5	acid	acid	NOUN
mkg-58282	52	6	sequence	sequence	NOUN
mkg-58282	52	7	of	of	ADP
mkg-58282	52	8	bfgf	bfgf	NOUN
mkg-58282	52	9	-	-	PUNCT
mkg-58282	52	10	ph	ph	ADJ
mkg-58282	52	11	,	,	PUNCT
mkg-58282	52	12	shown	show	VERB
mkg-58282	52	13	from	from	ADP
mkg-58282	52	14	nto	nto	PROPN
mkg-58282	52	15	c	c	PROPN
mkg-58282	52	16	-	-	PUNCT
mkg-58282	52	17	terminus	terminus	NOUN
mkg-58282	52	18	.	.	PUNCT
mkg-58282	53	1	ala1	ala1	PROPN
mkg-58282	53	2	–	–	PUNCT
mkg-58282	53	3	ser154	ser154	PROPN
mkg-58282	53	4	:	:	PUNCT
mkg-58282	53	5	bfgf	bfgf	NOUN
mkg-58282	53	6	domain	domain	NOUN
mkg-58282	53	7	(	(	PUNCT
mkg-58282	53	8	blue	blue	PROPN
mkg-58282	53	9	)	)	PUNCT
mkg-58282	53	10	;	;	PUNCT
mkg-58282	53	11	gly155	gly155	PROPN
mkg-58282	53	12	–	–	PUNCT
mkg-58282	53	13	ser169	ser169	PROPN
mkg-58282	53	14	:	:	PUNCT
mkg-58282	53	15	pentadeca	pentadeca	NOUN
mkg-58282	53	16	peptide	peptide	NOUN
mkg-58282	53	17	linker	linker	NOUN
mkg-58282	53	18	(	(	PUNCT
mkg-58282	53	19	red	red	PROPN
mkg-58282	53	20	)	)	PUNCT
mkg-58282	53	21	;	;	PUNCT
mkg-58282	53	22	his170	his170	PROPN
mkg-58282	53	23	–	–	PUNCT
mkg-58282	53	24	his175	his175	NOUN
mkg-58282	53	25	:	:	PUNCT
mkg-58282	53	26	hexahistidine	hexahistidine	NOUN
mkg-58282	53	27	tag	tag	NOUN
mkg-58282	53	28	(	(	PUNCT
mkg-58282	53	29	green	green	ADJ
mkg-58282	53	30	)	)	PUNCT
mkg-58282	53	31	.	.	PUNCT
mkg-58282	54	1	copyright	copyright	NOUN
mkg-58282	54	2	©	©	PROPN
mkg-58282	54	3	2025	2025	NUM
mkg-58282	54	4	dental	dental	ADJ
mkg-58282	54	5	journal	journal	NOUN
mkg-58282	54	6	(	(	PUNCT
mkg-58282	54	7	majalah	majalah	PROPN
mkg-58282	54	8	kedokteran	kedokteran	PROPN
mkg-58282	54	9	gigi	gigi	PROPN
mkg-58282	54	10	)	)	PUNCT
mkg-58282	55	1	p	p	X
mkg-58282	55	2	-	-	PUNCT
mkg-58282	55	3	issn	issn	NOUN
mkg-58282	55	4	:	:	PUNCT
mkg-58282	55	5	1978	1978	NUM
mkg-58282	55	6	-	-	SYM
mkg-58282	55	7	3728	3728	NUM
mkg-58282	55	8	;	;	PUNCT
mkg-58282	55	9	e	e	X
mkg-58282	55	10	-	-	PUNCT
mkg-58282	55	11	issn	issn	ADJ
mkg-58282	55	12	:	:	PUNCT
mkg-58282	55	13	2442	2442	NUM
mkg-58282	55	14	-	-	SYM
mkg-58282	55	15	9740	9740	NUM
mkg-58282	55	16	.	.	PUNCT
mkg-58282	56	1	accredited	accredit	VERB
mkg-58282	56	2	no	no	INTJ
mkg-58282	56	3	.	.	PROPN
mkg-58282	57	1	158	158	NUM
mkg-58282	57	2	/	/	SYM
mkg-58282	57	3	e	e	NOUN
mkg-58282	57	4	/	/	SYM
mkg-58282	57	5	kpt/2021	kpt/2021	NOUN
mkg-58282	57	6	.	.	PUNCT
mkg-58282	58	1	open	open	ADJ
mkg-58282	58	2	access	access	NOUN
mkg-58282	58	3	under	under	ADP
mkg-58282	58	4	cc	cc	NOUN
mkg-58282	58	5	-	-	PUNCT
mkg-58282	58	6	by	by	ADP
mkg-58282	58	7	-	-	PUNCT
mkg-58282	58	8	sa	sa	NOUN
mkg-58282	58	9	license	license	NOUN
mkg-58282	58	10	.	.	PUNCT
mkg-58282	59	1	available	available	ADJ
mkg-58282	59	2	at	at	ADP
mkg-58282	59	3	https://e-journal.unair.ac.id/mkg/index	https://e-journal.unair.ac.id/mkg/index	NOUN
mkg-58282	59	4	doi	doi	NOUN
mkg-58282	59	5	:	:	PUNCT
mkg-58282	59	6	10.20473	10.20473	NUM
mkg-58282	59	7	/	/	SYM
mkg-58282	59	8	j.djmkg.v58.i3.p219–223	j.djmkg.v58.i3.p219–223	PROPN
mkg-58282	59	9	https://github.com/deepmind/alphafold	https://github.com/deepmind/alphafold	PROPN
mkg-58282	59	10	https://e-journal.unair.ac.id/mkg/index	https://e-journal.unair.ac.id/mkg/index	PROPN
mkg-58282	59	11	https://doi.org/10.20473/j.djmkg.v58.i3.p219-223	https://doi.org/10.20473/j.djmkg.v58.i3.p219-223	VERB
mkg-58282	59	12	221widyantoro	221widyantoro	PROPN
mkg-58282	59	13	et	et	NOUN
mkg-58282	59	14	al	al	PROPN
mkg-58282	59	15	.	.	PROPN
mkg-58282	59	16	dent	dent	PROPN
mkg-58282	59	17	.	.	PUNCT
mkg-58282	60	1	j.	j.	PROPN
mkg-58282	60	2	(	(	PUNCT
mkg-58282	60	3	majalah	majalah	PROPN
mkg-58282	60	4	kedokteran	kedokteran	PROPN
mkg-58282	60	5	gigi	gigi	PROPN
mkg-58282	60	6	)	)	PUNCT
mkg-58282	60	7	2025	2025	NUM
mkg-58282	60	8	september	september	PROPN
mkg-58282	60	9	;	;	PUNCT
mkg-58282	60	10	58(3	58(3	NUM
mkg-58282	60	11	):	):	PUNCT
mkg-58282	60	12	219–223	219–223	NUM
mkg-58282	60	13	results	result	NOUN
mkg-58282	60	14	figure	figure	VERB
mkg-58282	60	15	2a	2a	NUM
mkg-58282	60	16	shows	show	VERB
mkg-58282	60	17	the	the	DET
mkg-58282	60	18	results	result	NOUN
mkg-58282	60	19	of	of	ADP
mkg-58282	60	20	the	the	DET
mkg-58282	60	21	sds	sds	NOUN
mkg-58282	60	22	-	-	PUNCT
mkg-58282	60	23	page	page	NOUN
mkg-58282	60	24	analysis	analysis	NOUN
mkg-58282	60	25	of	of	ADP
mkg-58282	60	26	the	the	DET
mkg-58282	60	27	protein	protein	NOUN
mkg-58282	60	28	after	after	ADP
mkg-58282	60	29	purification	purification	NOUN
mkg-58282	60	30	by	by	ADP
mkg-58282	60	31	metal	metal	NOUN
mkg-58282	60	32	chelate	chelate	NOUN
mkg-58282	60	33	affinity	affinity	NOUN
mkg-58282	60	34	chromatography	chromatography	NOUN
mkg-58282	60	35	.	.	PUNCT
mkg-58282	61	1	the	the	DET
mkg-58282	61	2	protein	protein	NOUN
mkg-58282	61	3	separated	separate	VERB
mkg-58282	61	4	into	into	ADP
mkg-58282	61	5	a	a	DET
mkg-58282	61	6	single	single	ADJ
mkg-58282	61	7	bold	bold	ADJ
mkg-58282	61	8	band	band	NOUN
mkg-58282	61	9	at	at	ADP
mkg-58282	61	10	19	19	NUM
mkg-58282	61	11	kda	kda	NOUN
mkg-58282	61	12	.	.	PUNCT
mkg-58282	62	1	this	this	DET
mkg-58282	62	2	molecular	molecular	ADJ
mkg-58282	62	3	weight	weight	NOUN
mkg-58282	62	4	matched	match	VERB
mkg-58282	62	5	the	the	DET
mkg-58282	62	6	predicted	predict	VERB
mkg-58282	62	7	value	value	NOUN
mkg-58282	62	8	based	base	VERB
mkg-58282	62	9	on	on	ADP
mkg-58282	62	10	the	the	DET
mkg-58282	62	11	amino	amino	NOUN
mkg-58282	62	12	acid	acid	NOUN
mkg-58282	62	13	sequence	sequence	NOUN
mkg-58282	62	14	of	of	ADP
mkg-58282	62	15	bfgf	bfgf	NOUN
mkg-58282	62	16	-	-	PUNCT
mkg-58282	62	17	ph	ph	ADJ
mkg-58282	62	18	,	,	PUNCT
mkg-58282	62	19	leading	lead	VERB
mkg-58282	62	20	us	we	PRON
mkg-58282	62	21	to	to	PART
mkg-58282	62	22	conclude	conclude	VERB
mkg-58282	62	23	that	that	SCONJ
mkg-58282	62	24	bfgf	bfgf	NOUN
mkg-58282	62	25	-	-	PUNCT
mkg-58282	62	26	ph	ph	NOUN
mkg-58282	62	27	was	be	AUX
mkg-58282	62	28	prepared	prepare	VERB
mkg-58282	62	29	as	as	SCONJ
mkg-58282	62	30	expected	expect	VERB
mkg-58282	62	31	.	.	PUNCT
mkg-58282	63	1	however	however	ADV
mkg-58282	63	2	,	,	PUNCT
mkg-58282	63	3	several	several	ADJ
mkg-58282	63	4	faint	faint	ADJ
mkg-58282	63	5	bands	band	NOUN
mkg-58282	63	6	were	be	AUX
mkg-58282	63	7	also	also	ADV
mkg-58282	63	8	observed	observe	VERB
mkg-58282	63	9	.	.	PUNCT
mkg-58282	64	1	these	these	DET
mkg-58282	64	2	bands	band	NOUN
mkg-58282	64	3	were	be	AUX
mkg-58282	64	4	attributed	attribute	VERB
mkg-58282	64	5	to	to	ADP
mkg-58282	64	6	endogenous	endogenous	ADJ
mkg-58282	64	7	bacterial	bacterial	ADJ
mkg-58282	64	8	proteins	protein	NOUN
mkg-58282	64	9	,	,	PUNCT
mkg-58282	64	10	which	which	PRON
mkg-58282	64	11	were	be	AUX
mkg-58282	64	12	reduced	reduce	VERB
mkg-58282	64	13	using	use	VERB
mkg-58282	64	14	the	the	DET
mkg-58282	64	15	dilution	dilution	NOUN
mkg-58282	64	16	method	method	NOUN
mkg-58282	64	17	(	(	PUNCT
mkg-58282	64	18	figure	figure	NOUN
mkg-58282	64	19	2b	2b	NOUN
mkg-58282	64	20	)	)	PUNCT
mkg-58282	64	21	,	,	PUNCT
mkg-58282	64	22	as	as	SCONJ
mkg-58282	64	23	described	describe	VERB
mkg-58282	64	24	later	later	ADV
mkg-58282	64	25	.	.	PUNCT
mkg-58282	65	1	the	the	DET
mkg-58282	65	2	bfgf	bfgf	NOUN
mkg-58282	65	3	-	-	PUNCT
mkg-58282	65	4	ph	ph	NOUN
mkg-58282	65	5	obtained	obtain	VERB
mkg-58282	65	6	as	as	ADP
mkg-58282	65	7	described	describe	VERB
mkg-58282	65	8	above	above	ADV
mkg-58282	65	9	was	be	AUX
mkg-58282	65	10	dialyzed	dialyze	VERB
mkg-58282	65	11	against	against	ADP
mkg-58282	65	12	20	20	NUM
mkg-58282	65	13	mm	mm	NOUN
mkg-58282	65	14	citrate	citrate	NOUN
mkg-58282	65	15	buffer	buffer	NOUN
mkg-58282	65	16	(	(	PUNCT
mkg-58282	65	17	ph	ph	NOUN
mkg-58282	65	18	5.0	5.0	NUM
mkg-58282	65	19	)	)	PUNCT
mkg-58282	65	20	containing	contain	VERB
mkg-58282	65	21	500	500	NUM
mkg-58282	65	22	mm	mm	NOUN
mkg-58282	65	23	nacl	nacl	NOUN
mkg-58282	65	24	.	.	PUNCT
mkg-58282	66	1	the	the	DET
mkg-58282	66	2	final	final	ADJ
mkg-58282	66	3	product	product	NOUN
mkg-58282	66	4	concentration	concentration	NOUN
mkg-58282	66	5	was	be	AUX
mkg-58282	66	6	243	243	NUM
mkg-58282	66	7	µg/	µg/	NOUN
mkg-58282	66	8	ml	ml	NOUN
mkg-58282	66	9	.	.	PUNCT
mkg-58282	67	1	compared	compare	VERB
mkg-58282	67	2	with	with	ADP
mkg-58282	67	3	the	the	DET
mkg-58282	67	4	cd	cd	PROPN
mkg-58282	67	5	spectrum	spectrum	NOUN
mkg-58282	67	6	generated	generate	VERB
mkg-58282	67	7	for	for	ADP
mkg-58282	67	8	the	the	DET
mkg-58282	67	9	computationally	computationally	ADV
mkg-58282	67	10	predicted	predict	VERB
mkg-58282	67	11	bfgf	bfgf	ADJ
mkg-58282	67	12	-	-	PUNCT
mkg-58282	67	13	ph	ph	ADJ
mkg-58282	67	14	structure	structure	NOUN
mkg-58282	67	15	(	(	PUNCT
mkg-58282	67	16	figure	figure	NOUN
mkg-58282	67	17	3a	3a	NUM
mkg-58282	67	18	)	)	PUNCT
mkg-58282	67	19	,	,	PUNCT
mkg-58282	67	20	the	the	DET
mkg-58282	67	21	experimental	experimental	ADJ
mkg-58282	67	22	cd	cd	PROPN
mkg-58282	67	23	spectrum	spectrum	NOUN
mkg-58282	67	24	of	of	ADP
mkg-58282	67	25	this	this	DET
mkg-58282	67	26	protein	protein	NOUN
mkg-58282	67	27	(	(	PUNCT
mkg-58282	67	28	figure	figure	NOUN
mkg-58282	67	29	3b	3b	NUM
mkg-58282	67	30	)	)	PUNCT
mkg-58282	67	31	had	have	VERB
mkg-58282	67	32	a	a	DET
mkg-58282	67	33	remarkably	remarkably	ADV
mkg-58282	67	34	different	different	ADJ
mkg-58282	67	35	shape	shape	NOUN
mkg-58282	67	36	.	.	PUNCT
mkg-58282	68	1	the	the	DET
mkg-58282	68	2	experimental	experimental	ADJ
mkg-58282	68	3	spectrum	spectrum	NOUN
mkg-58282	68	4	showed	show	VERB
mkg-58282	68	5	a	a	DET
mkg-58282	68	6	small	small	ADJ
mkg-58282	68	7	but	but	CCONJ
mkg-58282	68	8	distinct	distinct	ADJ
mkg-58282	68	9	cotton	cotton	NOUN
mkg-58282	68	10	effect	effect	NOUN
mkg-58282	68	11	at	at	ADP
mkg-58282	68	12	approximately	approximately	ADV
mkg-58282	68	13	208	208	NUM
mkg-58282	68	14	and	and	CCONJ
mkg-58282	68	15	220	220	NUM
mkg-58282	68	16	nm	nm	NOUN
mkg-58282	68	17	,	,	PUNCT
mkg-58282	68	18	suggesting	suggest	VERB
mkg-58282	68	19	the	the	DET
mkg-58282	68	20	presence	presence	NOUN
mkg-58282	68	21	of	of	ADP
mkg-58282	68	22	an	an	DET
mkg-58282	68	23	α	α	NOUN
mkg-58282	68	24	-	-	ADJ
mkg-58282	68	25	helical	helical	ADJ
mkg-58282	68	26	structure	structure	NOUN
mkg-58282	68	27	.	.	PUNCT
mkg-58282	69	1	in	in	ADP
mkg-58282	69	2	contrast	contrast	NOUN
mkg-58282	69	3	,	,	PUNCT
mkg-58282	69	4	the	the	DET
mkg-58282	69	5	cd	cd	PROPN
mkg-58282	69	6	spectrum	spectrum	NOUN
mkg-58282	69	7	of	of	ADP
mkg-58282	69	8	bfgf	bfgf	NOUN
mkg-58282	69	9	-	-	PUNCT
mkg-58282	69	10	ph	ph	NOUN
mkg-58282	69	11	dialyzed	dialyze	VERB
mkg-58282	69	12	against	against	ADP
mkg-58282	69	13	20	20	NUM
mkg-58282	69	14	mm	mm	NOUN
mkg-58282	69	15	citrate	citrate	NOUN
mkg-58282	69	16	buffer	buffer	NOUN
mkg-58282	69	17	(	(	PUNCT
mkg-58282	69	18	ph	ph	NOUN
mkg-58282	69	19	5.0	5.0	NUM
mkg-58282	69	20	)	)	PUNCT
mkg-58282	69	21	without	without	ADP
mkg-58282	69	22	500	500	NUM
mkg-58282	69	23	mm	mm	NOUN
mkg-58282	69	24	figure	figure	NOUN
mkg-58282	69	25	2	2	NUM
mkg-58282	69	26	.	.	PUNCT
mkg-58282	69	27	sodium	sodium	NOUN
mkg-58282	69	28	dodecyl	dodecyl	PROPN
mkg-58282	69	29	sulfate	sulfate	NOUN
mkg-58282	69	30	-	-	PUNCT
mkg-58282	69	31	polyacrylamide	polyacrylamide	NOUN
mkg-58282	69	32	gel	gel	NOUN
mkg-58282	69	33	electrophoresis	electrophoresis	NOUN
mkg-58282	69	34	analysis	analysis	NOUN
mkg-58282	69	35	of	of	ADP
mkg-58282	69	36	bfgf	bfgf	NOUN
mkg-58282	69	37	-	-	PUNCT
mkg-58282	69	38	ph	ph	NOUN
mkg-58282	69	39	.	.	PUNCT
mkg-58282	70	1	(	(	PUNCT
mkg-58282	70	2	a	a	X
mkg-58282	70	3	)	)	PUNCT
mkg-58282	70	4	lane	lane	NOUN
mkg-58282	70	5	1	1	NUM
mkg-58282	70	6	:	:	PUNCT
mkg-58282	70	7	molecular	molecular	ADJ
mkg-58282	70	8	weight	weight	NOUN
mkg-58282	70	9	standard	standard	NOUN
mkg-58282	70	10	;	;	PUNCT
mkg-58282	70	11	lane	lane	NOUN
mkg-58282	70	12	2	2	NUM
mkg-58282	70	13	:	:	PUNCT
mkg-58282	70	14	bfgf	bfgf	NOUN
mkg-58282	70	15	-	-	PUNCT
mkg-58282	70	16	ph	ph	NOUN
mkg-58282	70	17	obtained	obtain	VERB
mkg-58282	70	18	after	after	ADP
mkg-58282	70	19	affinity	affinity	NOUN
mkg-58282	70	20	purification	purification	NOUN
mkg-58282	70	21	.	.	PUNCT
mkg-58282	71	1	(	(	PUNCT
mkg-58282	71	2	b	b	X
mkg-58282	71	3	)	)	PUNCT
mkg-58282	71	4	lane	lane	NOUN
mkg-58282	71	5	3	3	NUM
mkg-58282	71	6	:	:	PUNCT
mkg-58282	71	7	molecular	molecular	ADJ
mkg-58282	71	8	weight	weight	NOUN
mkg-58282	71	9	standard	standard	NOUN
mkg-58282	71	10	;	;	PUNCT
mkg-58282	71	11	lane	lane	NOUN
mkg-58282	71	12	4	4	NUM
mkg-58282	71	13	:	:	PUNCT
mkg-58282	71	14	bfgf	bfgf	ADJ
mkg-58282	71	15	-	-	PUNCT
mkg-58282	71	16	ph	ph	ADJ
mkg-58282	71	17	refolded	refolde	VERB
mkg-58282	71	18	using	use	VERB
mkg-58282	71	19	the	the	DET
mkg-58282	71	20	dilution	dilution	NOUN
mkg-58282	71	21	method	method	NOUN
mkg-58282	71	22	and	and	CCONJ
mkg-58282	71	23	concentrated	concentrate	VERB
mkg-58282	71	24	via	via	ADP
mkg-58282	71	25	affinity	affinity	NOUN
mkg-58282	71	26	chromatography	chromatography	NOUN
mkg-58282	71	27	.	.	PUNCT
mkg-58282	72	1	arrowheads	arrowhead	NOUN
mkg-58282	72	2	indicate	indicate	VERB
mkg-58282	72	3	the	the	DET
mkg-58282	72	4	bfgf	bfgf	ADJ
mkg-58282	72	5	-	-	PUNCT
mkg-58282	72	6	ph	ph	ADJ
mkg-58282	72	7	band	band	NOUN
mkg-58282	72	8	.	.	PUNCT
mkg-58282	73	1	figure	figure	VERB
mkg-58282	73	2	3	3	NUM
mkg-58282	73	3	.	.	PUNCT
mkg-58282	74	1	far	far	ADJ
mkg-58282	74	2	-	-	PUNCT
mkg-58282	74	3	ultraviolet	ultraviolet	NOUN
mkg-58282	74	4	cd	cd	NOUN
mkg-58282	74	5	spectra	spectra	NOUN
mkg-58282	74	6	of	of	ADP
mkg-58282	74	7	bfgf	bfgf	NOUN
mkg-58282	74	8	-	-	PUNCT
mkg-58282	74	9	ph	ph	ADJ
mkg-58282	74	10	.	.	PUNCT
mkg-58282	75	1	(	(	PUNCT
mkg-58282	75	2	a	a	X
mkg-58282	75	3	)	)	PUNCT
mkg-58282	75	4	bfgf	bfgf	ADJ
mkg-58282	75	5	-	-	PUNCT
mkg-58282	75	6	ph	ph	ADJ
mkg-58282	75	7	structure	structure	NOUN
mkg-58282	75	8	predicted	predict	VERB
mkg-58282	75	9	by	by	ADP
mkg-58282	75	10	alphafold2	alphafold2	PROPN
mkg-58282	75	11	.	.	PUNCT
mkg-58282	76	1	the	the	DET
mkg-58282	76	2	cd	cd	PROPN
mkg-58282	76	3	spectrum	spectrum	NOUN
mkg-58282	76	4	was	be	AUX
mkg-58282	76	5	generated	generate	VERB
mkg-58282	76	6	as	as	ADP
mkg-58282	76	7	a	a	DET
mkg-58282	76	8	reference	reference	NOUN
mkg-58282	76	9	using	use	VERB
mkg-58282	76	10	the	the	DET
mkg-58282	76	11	atomic	atomic	ADJ
mkg-58282	76	12	coordinate	coordinate	NOUN
mkg-58282	76	13	file	file	NOUN
mkg-58282	76	14	of	of	ADP
mkg-58282	76	15	this	this	DET
mkg-58282	76	16	structure	structure	NOUN
mkg-58282	76	17	.	.	PUNCT
mkg-58282	77	1	(	(	PUNCT
mkg-58282	77	2	b	b	X
mkg-58282	77	3	–	–	PUNCT
mkg-58282	77	4	d	d	NOUN
mkg-58282	77	5	)	)	PUNCT
mkg-58282	77	6	circular	circular	ADJ
mkg-58282	77	7	dichroism	dichroism	NOUN
mkg-58282	77	8	spectra	spectra	NOUN
mkg-58282	77	9	recorded	record	VERB
mkg-58282	77	10	for	for	ADP
mkg-58282	77	11	bfgf	bfgf	NOUN
mkg-58282	77	12	-	-	PUNCT
mkg-58282	77	13	ph	ph	NOUN
mkg-58282	77	14	dialyzed	dialyze	VERB
mkg-58282	77	15	in	in	ADP
mkg-58282	77	16	20	20	NUM
mkg-58282	77	17	mm	mm	NOUN
mkg-58282	77	18	citrate	citrate	NOUN
mkg-58282	77	19	buffer	buffer	NOUN
mkg-58282	77	20	(	(	PUNCT
mkg-58282	77	21	b	b	NOUN
mkg-58282	77	22	)	)	PUNCT
mkg-58282	77	23	with	with	ADP
mkg-58282	77	24	or	or	CCONJ
mkg-58282	77	25	(	(	PUNCT
mkg-58282	77	26	c	c	NOUN
mkg-58282	77	27	)	)	PUNCT
mkg-58282	77	28	without	without	ADP
mkg-58282	77	29	500	500	NUM
mkg-58282	77	30	mm	mm	NOUN
mkg-58282	77	31	nacl	nacl	NOUN
mkg-58282	77	32	and	and	CCONJ
mkg-58282	77	33	(	(	PUNCT
mkg-58282	77	34	d	d	NOUN
mkg-58282	77	35	)	)	PUNCT
mkg-58282	77	36	bfgf	bfgf	ADJ
mkg-58282	77	37	-	-	PUNCT
mkg-58282	77	38	ph	ph	NOUN
mkg-58282	77	39	refolded	refolde	VERB
mkg-58282	77	40	in	in	ADP
mkg-58282	77	41	a	a	DET
mkg-58282	77	42	dilute	dilute	NOUN
mkg-58282	77	43	solution	solution	NOUN
mkg-58282	77	44	and	and	CCONJ
mkg-58282	77	45	concentrated	concentrate	VERB
mkg-58282	77	46	via	via	ADP
mkg-58282	77	47	affinity	affinity	NOUN
mkg-58282	77	48	chromatography	chromatography	NOUN
mkg-58282	77	49	.	.	PUNCT
mkg-58282	78	1	the	the	DET
mkg-58282	78	2	computationally	computationally	ADV
mkg-58282	78	3	predicted	predict	VERB
mkg-58282	78	4	reference	reference	NOUN
mkg-58282	78	5	spectrum	spectrum	NOUN
mkg-58282	78	6	was	be	AUX
mkg-58282	78	7	adjusted	adjust	VERB
mkg-58282	78	8	to	to	ADP
mkg-58282	78	9	an	an	DET
mkg-58282	78	10	arbitrary	arbitrary	ADJ
mkg-58282	78	11	scale	scale	NOUN
mkg-58282	78	12	and	and	CCONJ
mkg-58282	78	13	is	be	AUX
mkg-58282	78	14	shown	show	VERB
mkg-58282	78	15	as	as	ADP
mkg-58282	78	16	a	a	DET
mkg-58282	78	17	dotted	dotted	ADJ
mkg-58282	78	18	line	line	NOUN
mkg-58282	78	19	.	.	PUNCT
mkg-58282	79	1	copyright	copyright	NOUN
mkg-58282	79	2	©	©	PROPN
mkg-58282	79	3	2025	2025	NUM
mkg-58282	79	4	dental	dental	ADJ
mkg-58282	79	5	journal	journal	NOUN
mkg-58282	79	6	(	(	PUNCT
mkg-58282	79	7	majalah	majalah	PROPN
mkg-58282	79	8	kedokteran	kedokteran	PROPN
mkg-58282	79	9	gigi	gigi	PROPN
mkg-58282	79	10	)	)	PUNCT
mkg-58282	80	1	p	p	X
mkg-58282	80	2	-	-	PUNCT
mkg-58282	80	3	issn	issn	NOUN
mkg-58282	80	4	:	:	PUNCT
mkg-58282	80	5	1978	1978	NUM
mkg-58282	80	6	-	-	SYM
mkg-58282	80	7	3728	3728	NUM
mkg-58282	80	8	;	;	PUNCT
mkg-58282	80	9	e	e	X
mkg-58282	80	10	-	-	PUNCT
mkg-58282	80	11	issn	issn	ADJ
mkg-58282	80	12	:	:	PUNCT
mkg-58282	80	13	2442	2442	NUM
mkg-58282	80	14	-	-	SYM
mkg-58282	80	15	9740	9740	NUM
mkg-58282	80	16	.	.	PUNCT
mkg-58282	81	1	accredited	accredit	VERB
mkg-58282	81	2	no	no	INTJ
mkg-58282	81	3	.	.	PROPN
mkg-58282	82	1	158	158	NUM
mkg-58282	82	2	/	/	SYM
mkg-58282	82	3	e	e	NOUN
mkg-58282	82	4	/	/	SYM
mkg-58282	82	5	kpt/2021	kpt/2021	NOUN
mkg-58282	82	6	.	.	PUNCT
mkg-58282	83	1	open	open	ADJ
mkg-58282	83	2	access	access	NOUN
mkg-58282	83	3	under	under	ADP
mkg-58282	83	4	cc	cc	NOUN
mkg-58282	83	5	-	-	PUNCT
mkg-58282	83	6	by	by	ADP
mkg-58282	83	7	-	-	PUNCT
mkg-58282	83	8	sa	sa	NOUN
mkg-58282	83	9	license	license	NOUN
mkg-58282	83	10	.	.	PUNCT
mkg-58282	84	1	available	available	ADJ
mkg-58282	84	2	at	at	ADP
mkg-58282	84	3	https://e-journal.unair.ac.id/mkg/index	https://e-journal.unair.ac.id/mkg/index	NOUN
mkg-58282	84	4	doi	doi	NOUN
mkg-58282	84	5	:	:	PUNCT
mkg-58282	84	6	10.20473	10.20473	NUM
mkg-58282	84	7	/	/	SYM
mkg-58282	84	8	j.djmkg.v58.i3.p219–223	j.djmkg.v58.i3.p219–223	NOUN
mkg-58282	84	9	https://e-journal.unair.ac.id/mkg/index	https://e-journal.unair.ac.id/mkg/index	NOUN
mkg-58282	84	10	https://doi.org/10.20473/j.djmkg.v58.i3.p219-223	https://doi.org/10.20473/j.djmkg.v58.i3.p219-223	VERB
mkg-58282	84	11	222	222	NUM
mkg-58282	84	12	widyantoro	widyantoro	NOUN
mkg-58282	84	13	et	et	PROPN
mkg-58282	84	14	al	al	PROPN
mkg-58282	84	15	.	.	PROPN
mkg-58282	84	16	dent	dent	PROPN
mkg-58282	84	17	.	.	PUNCT
mkg-58282	85	1	j.	j.	PROPN
mkg-58282	85	2	(	(	PUNCT
mkg-58282	85	3	majalah	majalah	PROPN
mkg-58282	85	4	kedokteran	kedokteran	PROPN
mkg-58282	85	5	gigi	gigi	PROPN
mkg-58282	85	6	)	)	PUNCT
mkg-58282	85	7	2025	2025	NUM
mkg-58282	85	8	september	september	PROPN
mkg-58282	85	9	;	;	PUNCT
mkg-58282	85	10	58(3	58(3	NUM
mkg-58282	85	11	):	):	PUNCT
mkg-58282	85	12	219–223	219–223	NUM
mkg-58282	85	13	nacl	nacl	NOUN
mkg-58282	85	14	had	have	VERB
mkg-58282	85	15	a	a	DET
mkg-58282	85	16	shape	shape	NOUN
mkg-58282	85	17	similar	similar	ADJ
mkg-58282	85	18	to	to	ADP
mkg-58282	85	19	the	the	DET
mkg-58282	85	20	predicted	predict	VERB
mkg-58282	85	21	one	one	NUM
mkg-58282	85	22	(	(	PUNCT
mkg-58282	85	23	figure	figure	NOUN
mkg-58282	85	24	3c	3c	NUM
mkg-58282	85	25	)	)	PUNCT
mkg-58282	85	26	.	.	PUNCT
mkg-58282	86	1	however	however	ADV
mkg-58282	86	2	,	,	PUNCT
mkg-58282	86	3	the	the	DET
mkg-58282	86	4	yield	yield	NOUN
mkg-58282	86	5	of	of	ADP
mkg-58282	86	6	soluble	soluble	ADJ
mkg-58282	86	7	bfgf	bfgf	NOUN
mkg-58282	86	8	-	-	PUNCT
mkg-58282	86	9	ph	ph	NOUN
mkg-58282	86	10	was	be	AUX
mkg-58282	86	11	extremely	extremely	ADV
mkg-58282	86	12	low	low	ADJ
mkg-58282	86	13	(	(	PUNCT
mkg-58282	86	14	38.4	38.4	NUM
mkg-58282	86	15	µg	µg	NOUN
mkg-58282	86	16	/	/	SYM
mkg-58282	86	17	ml	ml	NOUN
mkg-58282	86	18	)	)	PUNCT
mkg-58282	86	19	due	due	ADP
mkg-58282	86	20	to	to	ADP
mkg-58282	86	21	substantial	substantial	ADJ
mkg-58282	86	22	aggregation	aggregation	NOUN
mkg-58282	86	23	and	and	CCONJ
mkg-58282	86	24	precipitation	precipitation	NOUN
mkg-58282	86	25	during	during	ADP
mkg-58282	86	26	dialysis	dialysis	NOUN
mkg-58282	86	27	in	in	ADP
mkg-58282	86	28	the	the	DET
mkg-58282	86	29	low	low	ADJ
mkg-58282	86	30	-	-	PUNCT
mkg-58282	86	31	ionic	ionic	ADJ
mkg-58282	86	32	-	-	PUNCT
mkg-58282	86	33	strength	strength	NOUN
mkg-58282	86	34	buffer	buffer	NOUN
mkg-58282	86	35	solution	solution	NOUN
mkg-58282	86	36	.	.	PUNCT
mkg-58282	87	1	conversely	conversely	ADV
mkg-58282	87	2	,	,	PUNCT
mkg-58282	87	3	the	the	DET
mkg-58282	87	4	cd	cd	PROPN
mkg-58282	87	5	spectrum	spectrum	NOUN
mkg-58282	87	6	of	of	ADP
mkg-58282	87	7	bfgf	bfgf	NOUN
mkg-58282	87	8	-	-	PUNCT
mkg-58282	87	9	ph	ph	NOUN
mkg-58282	87	10	obtained	obtain	VERB
mkg-58282	87	11	using	use	VERB
mkg-58282	87	12	the	the	DET
mkg-58282	87	13	dilution	dilution	NOUN
mkg-58282	87	14	method	method	NOUN
mkg-58282	87	15	closely	closely	ADV
mkg-58282	87	16	resembled	resemble	VERB
mkg-58282	87	17	the	the	DET
mkg-58282	87	18	predicted	predict	VERB
mkg-58282	87	19	spectrum	spectrum	NOUN
mkg-58282	87	20	(	(	PUNCT
mkg-58282	87	21	figure	figure	NOUN
mkg-58282	87	22	3d	3d	NUM
mkg-58282	87	23	)	)	PUNCT
mkg-58282	87	24	.	.	PUNCT
mkg-58282	88	1	the	the	DET
mkg-58282	88	2	diluted	diluted	ADJ
mkg-58282	88	3	bfgf	bfgf	NOUN
mkg-58282	88	4	-	-	PUNCT
mkg-58282	88	5	ph	ph	NOUN
mkg-58282	88	6	was	be	AUX
mkg-58282	88	7	subjected	subject	VERB
mkg-58282	88	8	to	to	ADP
mkg-58282	88	9	metal	metal	NOUN
mkg-58282	88	10	chelate	chelate	NOUN
mkg-58282	88	11	affinity	affinity	NOUN
mkg-58282	88	12	chromatography	chromatography	NOUN
mkg-58282	88	13	to	to	PART
mkg-58282	88	14	concentrate	concentrate	VERB
mkg-58282	88	15	bfgf	bfgf	NOUN
mkg-58282	88	16	-	-	PUNCT
mkg-58282	88	17	ph	ph	ADJ
mkg-58282	88	18	and	and	CCONJ
mkg-58282	88	19	eliminate	eliminate	VERB
mkg-58282	88	20	bacterial	bacterial	ADJ
mkg-58282	88	21	proteins	protein	NOUN
mkg-58282	88	22	(	(	PUNCT
mkg-58282	88	23	figure	figure	NOUN
mkg-58282	88	24	2b	2b	NOUN
mkg-58282	88	25	)	)	PUNCT
mkg-58282	88	26	.	.	PUNCT
mkg-58282	89	1	the	the	DET
mkg-58282	89	2	final	final	ADJ
mkg-58282	89	3	concentration	concentration	NOUN
mkg-58282	89	4	of	of	ADP
mkg-58282	89	5	soluble	soluble	ADJ
mkg-58282	89	6	bfgf	bfgf	NOUN
mkg-58282	89	7	-	-	PUNCT
mkg-58282	89	8	ph	ph	NOUN
mkg-58282	89	9	was	be	AUX
mkg-58282	89	10	120	120	NUM
mkg-58282	89	11	µg/	µg/	NOUN
mkg-58282	89	12	ml	ml	ADP
mkg-58282	89	13	,	,	PUNCT
mkg-58282	89	14	corresponding	correspond	VERB
mkg-58282	89	15	to	to	ADP
mkg-58282	89	16	a	a	DET
mkg-58282	89	17	yield	yield	NOUN
mkg-58282	89	18	of	of	ADP
mkg-58282	89	19	3.0	3.0	NUM
mkg-58282	89	20	mg	mg	NOUN
mkg-58282	89	21	per	per	ADP
mkg-58282	89	22	liter	liter	NOUN
mkg-58282	89	23	of	of	ADP
mkg-58282	89	24	lb	lb	DET
mkg-58282	89	25	medium	medium	ADJ
mkg-58282	89	26	.	.	PUNCT
mkg-58282	90	1	discussion	discussion	NOUN
mkg-58282	90	2	high	high	ADJ
mkg-58282	90	3	-	-	PUNCT
mkg-58282	90	4	yield	yield	NOUN
mkg-58282	90	5	preparation	preparation	NOUN
mkg-58282	90	6	of	of	ADP
mkg-58282	90	7	recombinant	recombinant	ADJ
mkg-58282	90	8	chimeric	chimeric	ADJ
mkg-58282	90	9	proteins	protein	NOUN
mkg-58282	90	10	is	be	AUX
mkg-58282	90	11	a	a	DET
mkg-58282	90	12	major	major	ADJ
mkg-58282	90	13	challenge	challenge	NOUN
mkg-58282	90	14	for	for	ADP
mkg-58282	90	15	downstream	downstream	ADJ
mkg-58282	90	16	applications	application	NOUN
mkg-58282	90	17	.	.	PUNCT
mkg-58282	91	1	in	in	ADP
mkg-58282	91	2	this	this	DET
mkg-58282	91	3	study	study	NOUN
mkg-58282	91	4	,	,	PUNCT
mkg-58282	91	5	we	we	PRON
mkg-58282	91	6	demonstrated	demonstrate	VERB
mkg-58282	91	7	that	that	SCONJ
mkg-58282	91	8	the	the	DET
mkg-58282	91	9	yield	yield	NOUN
mkg-58282	91	10	of	of	ADP
mkg-58282	91	11	engineered	engineer	VERB
mkg-58282	91	12	bfgf	bfgf	NOUN
mkg-58282	91	13	(	(	PUNCT
mkg-58282	91	14	bfgf	bfgf	NOUN
mkg-58282	91	15	-	-	PUNCT
mkg-58282	91	16	ph	ph	NOUN
mkg-58282	91	17	)	)	PUNCT
mkg-58282	91	18	was	be	AUX
mkg-58282	91	19	drastically	drastically	ADV
mkg-58282	91	20	increased	increase	VERB
mkg-58282	91	21	using	use	VERB
mkg-58282	91	22	the	the	DET
mkg-58282	91	23	dilution	dilution	NOUN
mkg-58282	91	24	method	method	NOUN
mkg-58282	91	25	.	.	PUNCT
mkg-58282	92	1	this	this	PRON
mkg-58282	92	2	may	may	AUX
mkg-58282	92	3	be	be	AUX
mkg-58282	92	4	due	due	ADJ
mkg-58282	92	5	to	to	ADP
mkg-58282	92	6	the	the	DET
mkg-58282	92	7	effect	effect	NOUN
mkg-58282	92	8	of	of	ADP
mkg-58282	92	9	diluting	dilute	VERB
mkg-58282	92	10	the	the	DET
mkg-58282	92	11	protein	protein	NOUN
mkg-58282	92	12	solution	solution	NOUN
mkg-58282	92	13	,	,	PUNCT
mkg-58282	92	14	which	which	PRON
mkg-58282	92	15	increases	increase	VERB
mkg-58282	92	16	the	the	DET
mkg-58282	92	17	average	average	ADJ
mkg-58282	92	18	protein	protein	NOUN
mkg-58282	92	19	–	–	PUNCT
mkg-58282	92	20	protein	protein	NOUN
mkg-58282	92	21	distance	distance	NOUN
mkg-58282	92	22	and	and	CCONJ
mkg-58282	92	23	decreases	decrease	VERB
mkg-58282	92	24	the	the	DET
mkg-58282	92	25	probability	probability	NOUN
mkg-58282	92	26	of	of	ADP
mkg-58282	92	27	monomer	monomer	NOUN
mkg-58282	92	28	association	association	NOUN
mkg-58282	92	29	into	into	ADP
mkg-58282	92	30	aggregates	aggregate	NOUN
mkg-58282	92	31	.	.	PUNCT
mkg-58282	93	1	according	accord	VERB
mkg-58282	93	2	to	to	ADP
mkg-58282	93	3	our	our	PRON
mkg-58282	93	4	previous	previous	ADJ
mkg-58282	93	5	experience	experience	NOUN
mkg-58282	93	6	with	with	ADP
mkg-58282	93	7	recombinant	recombinant	ADJ
mkg-58282	93	8	protein	protein	NOUN
mkg-58282	93	9	synthesis	synthesis	NOUN
mkg-58282	93	10	,	,	PUNCT
mkg-58282	93	11	the	the	DET
mkg-58282	93	12	solubility	solubility	NOUN
mkg-58282	93	13	of	of	ADP
mkg-58282	93	14	bfgf	bfgf	NOUN
mkg-58282	93	15	is	be	AUX
mkg-58282	93	16	relatively	relatively	ADV
mkg-58282	93	17	high.14	high.14	PROPN
mkg-58282	93	18	however	however	ADV
mkg-58282	93	19	,	,	PUNCT
mkg-58282	93	20	bfgf	bfgf	NOUN
mkg-58282	93	21	-	-	PUNCT
mkg-58282	93	22	ph	ph	NOUN
mkg-58282	93	23	had	have	VERB
mkg-58282	93	24	a	a	DET
mkg-58282	93	25	propensity	propensity	NOUN
mkg-58282	93	26	to	to	PART
mkg-58282	93	27	aggregate	aggregate	VERB
mkg-58282	93	28	and	and	CCONJ
mkg-58282	93	29	precipitate	precipitate	VERB
mkg-58282	93	30	,	,	PUNCT
mkg-58282	93	31	particularly	particularly	ADV
mkg-58282	93	32	when	when	SCONJ
mkg-58282	93	33	dialyzed	dialyze	VERB
mkg-58282	93	34	for	for	ADP
mkg-58282	93	35	refolding	refolding	NOUN
mkg-58282	93	36	and	and	CCONJ
mkg-58282	93	37	buffer	buffer	NOUN
mkg-58282	93	38	exchange	exchange	NOUN
mkg-58282	93	39	.	.	PUNCT
mkg-58282	94	1	this	this	DET
mkg-58282	94	2	tendency	tendency	NOUN
mkg-58282	94	3	was	be	AUX
mkg-58282	94	4	likely	likely	ADJ
mkg-58282	94	5	due	due	ADP
mkg-58282	94	6	to	to	ADP
mkg-58282	94	7	the	the	DET
mkg-58282	94	8	presence	presence	NOUN
mkg-58282	94	9	of	of	ADP
mkg-58282	94	10	the	the	DET
mkg-58282	94	11	pentadeca	pentadeca	NOUN
mkg-58282	94	12	-	-	PUNCT
mkg-58282	94	13	peptide	peptide	NOUN
mkg-58282	94	14	linker	linker	NOUN
mkg-58282	94	15	and	and	CCONJ
mkg-58282	94	16	hexahistidine	hexahistidine	NOUN
mkg-58282	94	17	tag	tag	NOUN
mkg-58282	94	18	.	.	PUNCT
mkg-58282	95	1	these	these	DET
mkg-58282	95	2	peptides	peptide	NOUN
mkg-58282	95	3	may	may	AUX
mkg-58282	95	4	reduce	reduce	VERB
mkg-58282	95	5	the	the	DET
mkg-58282	95	6	hydrophilicity	hydrophilicity	NOUN
mkg-58282	95	7	of	of	ADP
mkg-58282	95	8	bfgf	bfgf	NOUN
mkg-58282	95	9	and	and	CCONJ
mkg-58282	95	10	increase	increase	VERB
mkg-58282	95	11	the	the	DET
mkg-58282	95	12	likelihood	likelihood	NOUN
mkg-58282	95	13	of	of	ADP
mkg-58282	95	14	interactions	interaction	NOUN
mkg-58282	95	15	between	between	ADP
mkg-58282	95	16	neighboring	neighboring	NOUN
mkg-58282	95	17	segments	segment	NOUN
mkg-58282	95	18	.	.	PUNCT
mkg-58282	96	1	however	however	ADV
mkg-58282	96	2	,	,	PUNCT
mkg-58282	96	3	hexahistidine	hexahistidine	NOUN
mkg-58282	96	4	is	be	AUX
mkg-58282	96	5	useful	useful	ADJ
mkg-58282	96	6	for	for	ADP
mkg-58282	96	7	protein	protein	NOUN
mkg-58282	96	8	purification	purification	NOUN
mkg-58282	96	9	via	via	ADP
mkg-58282	96	10	metal	metal	NOUN
mkg-58282	96	11	chelate	chelate	NOUN
mkg-58282	96	12	affinity	affinity	NOUN
mkg-58282	96	13	chromatography	chromatography	NOUN
mkg-58282	96	14	and	and	CCONJ
mkg-58282	96	15	for	for	ADP
mkg-58282	96	16	the	the	DET
mkg-58282	96	17	surface	surface	NOUN
mkg-58282	96	18	tethering	tethering	NOUN
mkg-58282	96	19	of	of	ADP
mkg-58282	96	20	proteins.6,20	proteins.6,20	PROPN
mkg-58282	96	21	the	the	DET
mkg-58282	96	22	linker	linker	NOUN
mkg-58282	96	23	peptide	peptide	NOUN
mkg-58282	96	24	is	be	AUX
mkg-58282	96	25	expected	expect	VERB
mkg-58282	96	26	to	to	PART
mkg-58282	96	27	increase	increase	VERB
mkg-58282	96	28	the	the	DET
mkg-58282	96	29	mobility	mobility	NOUN
mkg-58282	96	30	of	of	ADP
mkg-58282	96	31	the	the	DET
mkg-58282	96	32	bfgf	bfgf	NOUN
mkg-58282	96	33	domain	domain	NOUN
mkg-58282	96	34	,	,	PUNCT
mkg-58282	96	35	potentially	potentially	ADV
mkg-58282	96	36	preventing	prevent	VERB
mkg-58282	96	37	steric	steric	ADJ
mkg-58282	96	38	hindrance	hindrance	NOUN
mkg-58282	96	39	.	.	PUNCT
mkg-58282	97	1	protein	protein	NOUN
mkg-58282	97	2	aggregation	aggregation	NOUN
mkg-58282	97	3	is	be	AUX
mkg-58282	97	4	influenced	influence	VERB
mkg-58282	97	5	by	by	ADP
mkg-58282	97	6	many	many	ADJ
mkg-58282	97	7	factors	factor	NOUN
mkg-58282	97	8	,	,	PUNCT
mkg-58282	97	9	including	include	VERB
mkg-58282	97	10	protein	protein	NOUN
mkg-58282	97	11	structure	structure	NOUN
mkg-58282	97	12	,	,	PUNCT
mkg-58282	97	13	chemical	chemical	NOUN
mkg-58282	97	14	degradation	degradation	NOUN
mkg-58282	97	15	,	,	PUNCT
mkg-58282	97	16	temperature	temperature	NOUN
mkg-58282	97	17	and	and	CCONJ
mkg-58282	97	18	solution	solution	NOUN
mkg-58282	97	19	conditions.10	conditions.10	NOUN
mkg-58282	97	20	in	in	ADP
mkg-58282	97	21	this	this	DET
mkg-58282	97	22	study	study	NOUN
mkg-58282	97	23	,	,	PUNCT
mkg-58282	97	24	we	we	PRON
mkg-58282	97	25	attempted	attempt	VERB
mkg-58282	97	26	to	to	PART
mkg-58282	97	27	optimize	optimize	VERB
mkg-58282	97	28	solution	solution	NOUN
mkg-58282	97	29	conditions	condition	NOUN
mkg-58282	97	30	by	by	ADP
mkg-58282	97	31	using	use	VERB
mkg-58282	97	32	a	a	DET
mkg-58282	97	33	citrate	citrate	NOUN
mkg-58282	97	34	buffer	buffer	NOUN
mkg-58282	97	35	(	(	PUNCT
mkg-58282	97	36	ph	ph	NOUN
mkg-58282	97	37	5.0	5.0	NUM
mkg-58282	97	38	)	)	PUNCT
mkg-58282	97	39	instead	instead	ADV
mkg-58282	97	40	of	of	ADP
mkg-58282	97	41	a	a	DET
mkg-58282	97	42	neutral	neutral	ADJ
mkg-58282	97	43	pb	pb	X
mkg-58282	97	44	and	and	CCONJ
mkg-58282	97	45	by	by	ADP
mkg-58282	97	46	adding	add	VERB
mkg-58282	97	47	500	500	NUM
mkg-58282	97	48	mm	mm	PROPN
mkg-58282	97	49	nacl	nacl	NOUN
mkg-58282	97	50	to	to	PART
mkg-58282	97	51	buffer	buffer	VERB
mkg-58282	97	52	solutions	solution	NOUN
mkg-58282	97	53	.	.	PUNCT
mkg-58282	98	1	these	these	DET
mkg-58282	98	2	modifications	modification	NOUN
mkg-58282	98	3	were	be	AUX
mkg-58282	98	4	based	base	VERB
mkg-58282	98	5	on	on	ADP
mkg-58282	98	6	the	the	DET
mkg-58282	98	7	understanding	understanding	NOUN
mkg-58282	98	8	that	that	SCONJ
mkg-58282	98	9	protein	protein	NOUN
mkg-58282	98	10	association	association	NOUN
mkg-58282	98	11	involves	involve	VERB
mkg-58282	98	12	several	several	ADJ
mkg-58282	98	13	forces	force	NOUN
mkg-58282	98	14	,	,	PUNCT
mkg-58282	98	15	such	such	ADJ
mkg-58282	98	16	as	as	ADP
mkg-58282	98	17	electrostatic	electrostatic	ADJ
mkg-58282	98	18	attraction	attraction	NOUN
mkg-58282	98	19	,	,	PUNCT
mkg-58282	98	20	hydrogen	hydrogen	NOUN
mkg-58282	98	21	bonding	bonding	NOUN
mkg-58282	98	22	,	,	PUNCT
mkg-58282	98	23	van	van	PROPN
mkg-58282	98	24	der	der	NOUN
mkg-58282	98	25	waals	waal	NOUN
mkg-58282	98	26	forces	force	NOUN
mkg-58282	98	27	and	and	CCONJ
mkg-58282	98	28	hydrophobic	hydrophobic	NOUN
mkg-58282	98	29	interactions.10	interactions.10	NOUN
mkg-58282	98	30	however	however	ADV
mkg-58282	98	31	,	,	PUNCT
mkg-58282	98	32	despite	despite	SCONJ
mkg-58282	98	33	these	these	DET
mkg-58282	98	34	efforts	effort	NOUN
mkg-58282	98	35	,	,	PUNCT
mkg-58282	98	36	we	we	PRON
mkg-58282	98	37	were	be	AUX
mkg-58282	98	38	unable	unable	ADJ
mkg-58282	98	39	to	to	PART
mkg-58282	98	40	recover	recover	VERB
mkg-58282	98	41	large	large	ADJ
mkg-58282	98	42	amounts	amount	NOUN
mkg-58282	98	43	of	of	ADP
mkg-58282	98	44	soluble	soluble	ADJ
mkg-58282	98	45	and	and	CCONJ
mkg-58282	98	46	correctly	correctly	ADV
mkg-58282	98	47	folded	fold	VERB
mkg-58282	98	48	bfgf	bfgf	NOUN
mkg-58282	98	49	-	-	PUNCT
mkg-58282	98	50	ph	ph	ADJ
mkg-58282	98	51	.	.	NOUN
mkg-58282	99	1	in	in	ADP
mkg-58282	99	2	contrast	contrast	NOUN
mkg-58282	99	3	,	,	PUNCT
mkg-58282	99	4	when	when	SCONJ
mkg-58282	99	5	the	the	DET
mkg-58282	99	6	bfgf	bfgf	ADJ
mkg-58282	99	7	-	-	PUNCT
mkg-58282	99	8	ph	ph	ADJ
mkg-58282	99	9	concentration	concentration	NOUN
mkg-58282	99	10	was	be	AUX
mkg-58282	99	11	reduced	reduce	VERB
mkg-58282	99	12	100	100	NUM
mkg-58282	99	13	-	-	NUM
mkg-58282	99	14	fold	fold	ADJ
mkg-58282	99	15	,	,	PUNCT
mkg-58282	99	16	the	the	DET
mkg-58282	99	17	protein	protein	NOUN
mkg-58282	99	18	remained	remain	VERB
mkg-58282	99	19	soluble	soluble	ADJ
mkg-58282	99	20	during	during	ADP
mkg-58282	99	21	dialysis	dialysis	NOUN
mkg-58282	99	22	and	and	CCONJ
mkg-58282	99	23	folded	fold	VERB
mkg-58282	99	24	into	into	ADP
mkg-58282	99	25	its	its	PRON
mkg-58282	99	26	correct	correct	ADJ
mkg-58282	99	27	structure	structure	NOUN
mkg-58282	99	28	.	.	PUNCT
mkg-58282	100	1	accordingly	accordingly	ADV
mkg-58282	100	2	,	,	PUNCT
mkg-58282	100	3	we	we	PRON
mkg-58282	100	4	found	find	VERB
mkg-58282	100	5	this	this	DET
mkg-58282	100	6	procedure	procedure	NOUN
mkg-58282	100	7	to	to	PART
mkg-58282	100	8	be	be	AUX
mkg-58282	100	9	simple	simple	ADJ
mkg-58282	100	10	yet	yet	CCONJ
mkg-58282	100	11	effective	effective	ADJ
mkg-58282	100	12	.	.	PUNCT
mkg-58282	101	1	the	the	DET
mkg-58282	101	2	average	average	ADJ
mkg-58282	101	3	protein	protein	NOUN
mkg-58282	101	4	–	–	PUNCT
mkg-58282	101	5	protein	protein	NOUN
mkg-58282	101	6	distance	distance	NOUN
mkg-58282	101	7	in	in	ADP
mkg-58282	101	8	solution	solution	NOUN
mkg-58282	101	9	increases	increase	NOUN
mkg-58282	101	10	as	as	ADP
mkg-58282	101	11	concentration	concentration	NOUN
mkg-58282	101	12	decreases	decrease	NOUN
mkg-58282	101	13	,	,	PUNCT
mkg-58282	101	14	which	which	PRON
mkg-58282	101	15	likely	likely	ADV
mkg-58282	101	16	reduces	reduce	VERB
mkg-58282	101	17	the	the	DET
mkg-58282	101	18	probability	probability	NOUN
mkg-58282	101	19	of	of	ADP
mkg-58282	101	20	monomer	monomer	NOUN
mkg-58282	101	21	association	association	NOUN
mkg-58282	101	22	and	and	CCONJ
mkg-58282	101	23	oligomer	oligomer	ADJ
mkg-58282	101	24	growth	growth	NOUN
mkg-58282	101	25	into	into	ADP
mkg-58282	101	26	aggregates.10	aggregates.10	ADV
mkg-58282	101	27	the	the	DET
mkg-58282	101	28	diluted	diluted	ADJ
mkg-58282	101	29	bfgf	bfgf	NOUN
mkg-58282	101	30	-	-	PUNCT
mkg-58282	101	31	ph	ph	NOUN
mkg-58282	101	32	could	could	AUX
mkg-58282	101	33	also	also	ADV
mkg-58282	101	34	be	be	AUX
mkg-58282	101	35	easily	easily	ADV
mkg-58282	101	36	reconcentrated	reconcentrate	VERB
mkg-58282	101	37	using	use	VERB
mkg-58282	101	38	metal	metal	NOUN
mkg-58282	101	39	chelate	chelate	NOUN
mkg-58282	101	40	affinity	affinity	NOUN
mkg-58282	101	41	chromatography	chromatography	NOUN
mkg-58282	101	42	.	.	PUNCT
mkg-58282	102	1	in	in	ADP
mkg-58282	102	2	this	this	DET
mkg-58282	102	3	study	study	NOUN
mkg-58282	102	4	,	,	PUNCT
mkg-58282	102	5	we	we	PRON
mkg-58282	102	6	determined	determine	VERB
mkg-58282	102	7	protein	protein	NOUN
mkg-58282	102	8	concentration	concentration	NOUN
mkg-58282	102	9	using	use	VERB
mkg-58282	102	10	a	a	DET
mkg-58282	102	11	standard	standard	ADJ
mkg-58282	102	12	protein	protein	NOUN
mkg-58282	102	13	assay	assay	VERB
mkg-58282	102	14	,	,	PUNCT
mkg-58282	102	15	while	while	SCONJ
mkg-58282	102	16	correct	correct	ADJ
mkg-58282	102	17	folding	folding	NOUN
mkg-58282	102	18	was	be	AUX
mkg-58282	102	19	assessed	assess	VERB
mkg-58282	102	20	by	by	ADP
mkg-58282	102	21	cd	cd	PROPN
mkg-58282	102	22	spectroscopy	spectroscopy	NOUN
mkg-58282	102	23	.	.	PUNCT
mkg-58282	103	1	this	this	DET
mkg-58282	103	2	spectroscopic	spectroscopic	ADJ
mkg-58282	103	3	method	method	NOUN
mkg-58282	103	4	is	be	AUX
mkg-58282	103	5	useful	useful	ADJ
mkg-58282	103	6	for	for	ADP
mkg-58282	103	7	analyzing	analyze	VERB
mkg-58282	103	8	the	the	DET
mkg-58282	103	9	secondary	secondary	ADJ
mkg-58282	103	10	structures	structure	NOUN
mkg-58282	103	11	present	present	ADJ
mkg-58282	103	12	in	in	ADP
mkg-58282	103	13	a	a	DET
mkg-58282	103	14	protein	protein	NOUN
mkg-58282	103	15	of	of	ADP
mkg-58282	103	16	interest	interest	NOUN
mkg-58282	103	17	,	,	PUNCT
mkg-58282	103	18	although	although	SCONJ
mkg-58282	103	19	it	it	PRON
mkg-58282	103	20	does	do	AUX
mkg-58282	103	21	not	not	PART
mkg-58282	103	22	provide	provide	VERB
mkg-58282	103	23	information	information	NOUN
mkg-58282	103	24	on	on	ADP
mkg-58282	103	25	tertiary	tertiary	ADJ
mkg-58282	103	26	structure	structure	NOUN
mkg-58282	103	27	.	.	PUNCT
mkg-58282	104	1	in	in	ADP
mkg-58282	104	2	cd	cd	PROPN
mkg-58282	104	3	spectroscopic	spectroscopic	NOUN
mkg-58282	104	4	analysis	analysis	NOUN
mkg-58282	104	5	,	,	PUNCT
mkg-58282	104	6	a	a	DET
mkg-58282	104	7	reference	reference	NOUN
mkg-58282	104	8	spectrum	spectrum	NOUN
mkg-58282	104	9	of	of	ADP
mkg-58282	104	10	a	a	DET
mkg-58282	104	11	correctly	correctly	ADV
mkg-58282	104	12	folded	fold	VERB
mkg-58282	104	13	protein	protein	NOUN
mkg-58282	104	14	is	be	AUX
mkg-58282	104	15	required	require	VERB
mkg-58282	104	16	to	to	PART
mkg-58282	104	17	validate	validate	VERB
mkg-58282	104	18	the	the	DET
mkg-58282	104	19	structure	structure	NOUN
mkg-58282	104	20	of	of	ADP
mkg-58282	104	21	sample	sample	NOUN
mkg-58282	104	22	proteins	protein	NOUN
mkg-58282	104	23	.	.	PUNCT
mkg-58282	105	1	however	however	ADV
mkg-58282	105	2	,	,	PUNCT
mkg-58282	105	3	no	no	DET
mkg-58282	105	4	such	such	ADJ
mkg-58282	105	5	reference	reference	NOUN
mkg-58282	105	6	spectrum	spectrum	NOUN
mkg-58282	105	7	exists	exist	VERB
mkg-58282	105	8	for	for	ADP
mkg-58282	105	9	bfgf	bfgf	NOUN
mkg-58282	105	10	-	-	PUNCT
mkg-58282	105	11	ph	ph	ADJ
mkg-58282	105	12	,	,	PUNCT
mkg-58282	105	13	as	as	SCONJ
mkg-58282	105	14	its	its	PRON
mkg-58282	105	15	sequence	sequence	NOUN
mkg-58282	105	16	is	be	AUX
mkg-58282	105	17	artificial	artificial	ADJ
mkg-58282	105	18	and	and	CCONJ
mkg-58282	105	19	unique	unique	ADJ
mkg-58282	105	20	.	.	PUNCT
mkg-58282	106	1	for	for	ADP
mkg-58282	106	2	this	this	DET
mkg-58282	106	3	reason	reason	NOUN
mkg-58282	106	4	,	,	PUNCT
mkg-58282	106	5	we	we	PRON
mkg-58282	106	6	performed	perform	VERB
mkg-58282	106	7	a	a	DET
mkg-58282	106	8	computerbased	computerbase	VERB
mkg-58282	106	9	prediction	prediction	NOUN
mkg-58282	106	10	of	of	ADP
mkg-58282	106	11	a	a	DET
mkg-58282	106	12	cd	cd	NOUN
mkg-58282	106	13	spectrum	spectrum	NOUN
mkg-58282	106	14	using	use	VERB
mkg-58282	106	15	the	the	DET
mkg-58282	106	16	pdbmd2cd	pdbmd2cd	NOUN
mkg-58282	106	17	program.16	program.16	ADV
mkg-58282	106	18	this	this	DET
mkg-58282	106	19	program	program	NOUN
mkg-58282	106	20	provides	provide	VERB
mkg-58282	106	21	highly	highly	ADV
mkg-58282	106	22	accurate	accurate	ADJ
mkg-58282	106	23	cd	cd	NOUN
mkg-58282	106	24	spectra	spectra	NOUN
mkg-58282	106	25	for	for	ADP
mkg-58282	106	26	a	a	DET
mkg-58282	106	27	wide	wide	ADJ
mkg-58282	106	28	variety	variety	NOUN
mkg-58282	106	29	of	of	ADP
mkg-58282	106	30	protein	protein	NOUN
mkg-58282	106	31	samples.21	samples.21	NOUN
mkg-58282	106	32	the	the	DET
mkg-58282	106	33	cotton	cotton	NOUN
mkg-58282	106	34	effect	effect	NOUN
mkg-58282	106	35	observed	observe	VERB
mkg-58282	106	36	in	in	ADP
mkg-58282	106	37	the	the	DET
mkg-58282	106	38	predicted	predict	VERB
mkg-58282	106	39	spectrum	spectrum	NOUN
mkg-58282	106	40	was	be	AUX
mkg-58282	106	41	approximately	approximately	ADV
mkg-58282	106	42	three	three	NUM
mkg-58282	106	43	times	time	NOUN
mkg-58282	106	44	larger	large	ADJ
mkg-58282	106	45	than	than	ADP
mkg-58282	106	46	those	those	PRON
mkg-58282	106	47	in	in	ADP
mkg-58282	106	48	the	the	DET
mkg-58282	106	49	experimental	experimental	ADJ
mkg-58282	106	50	spectra	spectra	NOUN
mkg-58282	106	51	across	across	ADP
mkg-58282	106	52	the	the	DET
mkg-58282	106	53	entire	entire	ADJ
mkg-58282	106	54	wavelength	wavelength	NOUN
mkg-58282	106	55	range	range	NOUN
mkg-58282	106	56	,	,	PUNCT
mkg-58282	106	57	most	most	ADV
mkg-58282	106	58	likely	likely	ADJ
mkg-58282	106	59	due	due	ADP
mkg-58282	106	60	to	to	ADP
mkg-58282	106	61	the	the	DET
mkg-58282	106	62	presence	presence	NOUN
mkg-58282	106	63	of	of	ADP
mkg-58282	106	64	denatured	denature	VERB
mkg-58282	106	65	bfgf	bfgf	NOUN
mkg-58282	106	66	-	-	PUNCT
mkg-58282	106	67	ph	ph	NOUN
mkg-58282	106	68	in	in	ADP
mkg-58282	106	69	the	the	DET
mkg-58282	106	70	sample	sample	NOUN
mkg-58282	106	71	solutions	solution	NOUN
mkg-58282	106	72	.	.	PUNCT
mkg-58282	107	1	to	to	PART
mkg-58282	107	2	facilitate	facilitate	VERB
mkg-58282	107	3	direct	direct	ADJ
mkg-58282	107	4	comparison	comparison	NOUN
mkg-58282	107	5	,	,	PUNCT
mkg-58282	107	6	we	we	PRON
mkg-58282	107	7	used	use	VERB
mkg-58282	107	8	an	an	DET
mkg-58282	107	9	arbitrary	arbitrary	ADJ
mkg-58282	107	10	unit	unit	NOUN
mkg-58282	107	11	for	for	ADP
mkg-58282	107	12	the	the	DET
mkg-58282	107	13	predicted	predict	VERB
mkg-58282	107	14	spectrum	spectrum	NOUN
mkg-58282	107	15	in	in	ADP
mkg-58282	107	16	figure	figure	NOUN
mkg-58282	107	17	3	3	NUM
mkg-58282	107	18	(	(	PUNCT
mkg-58282	107	19	dotted	dotted	ADJ
mkg-58282	107	20	line	line	NOUN
mkg-58282	107	21	)	)	PUNCT
mkg-58282	107	22	,	,	PUNCT
mkg-58282	107	23	allowing	allow	VERB
mkg-58282	107	24	the	the	DET
mkg-58282	107	25	spectral	spectral	ADJ
mkg-58282	107	26	shapes	shape	NOUN
mkg-58282	107	27	to	to	PART
mkg-58282	107	28	be	be	AUX
mkg-58282	107	29	compared	compare	VERB
mkg-58282	107	30	with	with	ADP
mkg-58282	107	31	each	each	DET
mkg-58282	107	32	other	other	ADJ
mkg-58282	107	33	.	.	PUNCT
mkg-58282	108	1	this	this	DET
mkg-58282	108	2	approach	approach	NOUN
mkg-58282	108	3	ultimately	ultimately	ADV
mkg-58282	108	4	enabled	enable	VERB
mkg-58282	108	5	us	we	PRON
mkg-58282	108	6	to	to	PART
mkg-58282	108	7	identify	identify	VERB
mkg-58282	108	8	the	the	DET
mkg-58282	108	9	conditions	condition	NOUN
mkg-58282	108	10	under	under	ADP
mkg-58282	108	11	which	which	PRON
mkg-58282	108	12	soluble	soluble	ADJ
mkg-58282	108	13	and	and	CCONJ
mkg-58282	108	14	correctly	correctly	ADV
mkg-58282	108	15	folded	fold	VERB
mkg-58282	108	16	bfgf	bfgf	NOUN
mkg-58282	108	17	-	-	PUNCT
mkg-58282	108	18	ph	ph	NOUN
mkg-58282	108	19	could	could	AUX
mkg-58282	108	20	be	be	AUX
mkg-58282	108	21	obtained	obtain	VERB
mkg-58282	108	22	in	in	ADP
mkg-58282	108	23	sufficiently	sufficiently	ADV
mkg-58282	108	24	high	high	ADJ
mkg-58282	108	25	yields	yield	NOUN
mkg-58282	108	26	.	.	PUNCT
mkg-58282	109	1	in	in	ADP
mkg-58282	109	2	conclusion	conclusion	NOUN
mkg-58282	109	3	,	,	PUNCT
mkg-58282	109	4	the	the	DET
mkg-58282	109	5	dilution	dilution	NOUN
mkg-58282	109	6	method	method	NOUN
mkg-58282	109	7	used	use	VERB
mkg-58282	109	8	in	in	ADP
mkg-58282	109	9	this	this	DET
mkg-58282	109	10	study	study	NOUN
mkg-58282	109	11	effectively	effectively	ADV
mkg-58282	109	12	increased	increase	VERB
mkg-58282	109	13	the	the	DET
mkg-58282	109	14	yield	yield	NOUN
mkg-58282	109	15	of	of	ADP
mkg-58282	109	16	correctly	correctly	ADV
mkg-58282	109	17	folded	fold	VERB
mkg-58282	109	18	bfgfph	bfgfph	NOUN
mkg-58282	109	19	.	.	PUNCT
mkg-58282	110	1	protein	protein	NOUN
mkg-58282	110	2	aggregation	aggregation	NOUN
mkg-58282	110	3	is	be	AUX
mkg-58282	110	4	a	a	DET
mkg-58282	110	5	major	major	ADJ
mkg-58282	110	6	issue	issue	NOUN
mkg-58282	110	7	that	that	PRON
mkg-58282	110	8	frequently	frequently	ADV
mkg-58282	110	9	arises	arise	VERB
mkg-58282	110	10	during	during	ADP
mkg-58282	110	11	the	the	DET
mkg-58282	110	12	purification	purification	NOUN
mkg-58282	110	13	and	and	CCONJ
mkg-58282	110	14	refolding	refolding	NOUN
mkg-58282	110	15	of	of	ADP
mkg-58282	110	16	recombinant	recombinant	ADJ
mkg-58282	110	17	proteins	protein	NOUN
mkg-58282	110	18	.	.	PUNCT
mkg-58282	111	1	the	the	DET
mkg-58282	111	2	results	result	NOUN
mkg-58282	111	3	of	of	ADP
mkg-58282	111	4	this	this	DET
mkg-58282	111	5	study	study	NOUN
mkg-58282	111	6	provide	provide	VERB
mkg-58282	111	7	further	further	ADJ
mkg-58282	111	8	evidence	evidence	NOUN
mkg-58282	111	9	that	that	SCONJ
mkg-58282	111	10	the	the	DET
mkg-58282	111	11	dilution	dilution	NOUN
mkg-58282	111	12	method	method	NOUN
mkg-58282	111	13	can	can	AUX
mkg-58282	111	14	enhance	enhance	VERB
mkg-58282	111	15	the	the	DET
mkg-58282	111	16	yields	yield	NOUN
mkg-58282	111	17	of	of	ADP
mkg-58282	111	18	correctly	correctly	ADV
mkg-58282	111	19	folded	fold	VERB
mkg-58282	111	20	products	product	NOUN
mkg-58282	111	21	.	.	PUNCT
mkg-58282	112	1	while	while	SCONJ
mkg-58282	112	2	this	this	DET
mkg-58282	112	3	study	study	NOUN
mkg-58282	112	4	focused	focus	VERB
mkg-58282	112	5	on	on	ADP
mkg-58282	112	6	a	a	DET
mkg-58282	112	7	specific	specific	ADJ
mkg-58282	112	8	case	case	NOUN
mkg-58282	112	9	with	with	ADP
mkg-58282	112	10	bfgf	bfgf	NOUN
mkg-58282	112	11	-	-	PUNCT
mkg-58282	112	12	ph	ph	ADJ
mkg-58282	112	13	,	,	PUNCT
mkg-58282	112	14	optimal	optimal	ADJ
mkg-58282	112	15	dilution	dilution	NOUN
mkg-58282	112	16	conditions	condition	NOUN
mkg-58282	112	17	may	may	AUX
mkg-58282	112	18	vary	vary	VERB
mkg-58282	112	19	depending	depend	VERB
mkg-58282	112	20	on	on	ADP
mkg-58282	112	21	protein	protein	NOUN
mkg-58282	112	22	structure	structure	NOUN
mkg-58282	112	23	.	.	PUNCT
mkg-58282	113	1	further	further	ADJ
mkg-58282	113	2	studies	study	NOUN
mkg-58282	113	3	on	on	ADP
mkg-58282	113	4	a	a	DET
mkg-58282	113	5	broader	broad	ADJ
mkg-58282	113	6	range	range	NOUN
mkg-58282	113	7	of	of	ADP
mkg-58282	113	8	chimeric	chimeric	ADJ
mkg-58282	113	9	proteins	protein	NOUN
mkg-58282	113	10	will	will	AUX
mkg-58282	113	11	be	be	AUX
mkg-58282	113	12	necessary	necessary	ADJ
mkg-58282	113	13	to	to	PART
mkg-58282	113	14	verify	verify	VERB
mkg-58282	113	15	the	the	DET
mkg-58282	113	16	versatility	versatility	NOUN
mkg-58282	113	17	of	of	ADP
mkg-58282	113	18	this	this	DET
mkg-58282	113	19	method	method	NOUN
mkg-58282	113	20	.	.	PUNCT
mkg-58282	114	1	acknowledgement	acknowledgement	NOUN
mkg-58282	114	2	this	this	DET
mkg-58282	114	3	study	study	NOUN
mkg-58282	114	4	was	be	AUX
mkg-58282	114	5	supported	support	VERB
mkg-58282	114	6	by	by	ADP
mkg-58282	114	7	a	a	DET
mkg-58282	114	8	grant	grant	NOUN
mkg-58282	114	9	-	-	PUNCT
mkg-58282	114	10	in	in	ADP
mkg-58282	114	11	-	-	PUNCT
mkg-58282	114	12	aid	aid	NOUN
mkg-58282	114	13	for	for	ADP
mkg-58282	114	14	scientific	scientific	ADJ
mkg-58282	114	15	research	research	NOUN
mkg-58282	114	16	from	from	ADP
mkg-58282	114	17	the	the	DET
mkg-58282	114	18	japan	japan	PROPN
mkg-58282	114	19	society	society	PROPN
mkg-58282	114	20	for	for	ADP
mkg-58282	114	21	the	the	DET
mkg-58282	114	22	promotion	promotion	NOUN
mkg-58282	114	23	of	of	ADP
mkg-58282	114	24	science	science	NOUN
mkg-58282	114	25	(	(	PUNCT
mkg-58282	114	26	no	no	INTJ
mkg-58282	114	27	.	.	PUNCT
mkg-58282	114	28	20k09994	20k09994	NUM
mkg-58282	114	29	)	)	PUNCT
mkg-58282	114	30	.	.	PUNCT
mkg-58282	115	1	references	reference	NOUN
mkg-58282	115	2	1	1	NUM
mkg-58282	115	3	.	.	PUNCT
mkg-58282	116	1	zhu	zhu	PROPN
mkg-58282	116	2	w	w	PROPN
mkg-58282	116	3	,	,	PUNCT
mkg-58282	116	4	kong	kong	PROPN
mkg-58282	116	5	c	c	NOUN
mkg-58282	116	6	,	,	PUNCT
mkg-58282	116	7	pan	pan	PROPN
mkg-58282	116	8	f	f	PROPN
mkg-58282	116	9	,	,	PUNCT
mkg-58282	116	10	ouyang	ouyang	PROPN
mkg-58282	116	11	m	m	PROPN
mkg-58282	116	12	,	,	PUNCT
mkg-58282	116	13	sun	sun	PROPN
mkg-58282	116	14	k	k	PROPN
mkg-58282	116	15	,	,	PUNCT
mkg-58282	116	16	lu	lu	PROPN
mkg-58282	116	17	s.	s.	PROPN
mkg-58282	116	18	engineered	engineer	VERB
mkg-58282	116	19	collagen	collagen	NOUN
mkg-58282	116	20	-	-	PUNCT
mkg-58282	116	21	binding	bind	VERB
mkg-58282	116	22	bone	bone	NOUN
mkg-58282	116	23	morphogenetic	morphogenetic	ADJ
mkg-58282	116	24	protein-2	protein-2	NOUN
mkg-58282	116	25	incorporated	incorporate	VERB
mkg-58282	116	26	with	with	ADP
mkg-58282	116	27	platelet	platelet	ADJ
mkg-58282	116	28	-	-	PUNCT
mkg-58282	116	29	rich	rich	ADJ
mkg-58282	116	30	plasma	plasma	NOUN
mkg-58282	116	31	accelerates	accelerate	VERB
mkg-58282	116	32	lumbar	lumbar	ADJ
mkg-58282	116	33	fusion	fusion	NOUN
mkg-58282	116	34	in	in	ADP
mkg-58282	116	35	aged	aged	ADJ
mkg-58282	116	36	rats	rat	NOUN
mkg-58282	116	37	with	with	ADP
mkg-58282	116	38	osteopenia	osteopenia	NOUN
mkg-58282	116	39	.	.	PUNCT
mkg-58282	117	1	exp	exp	NOUN
mkg-58282	117	2	biol	biol	PROPN
mkg-58282	117	3	med	me	VERB
mkg-58282	117	4	.	.	PUNCT
mkg-58282	117	5	2021	2021	NUM
mkg-58282	117	6	;	;	PUNCT
mkg-58282	117	7	246(14	246(14	NUM
mkg-58282	117	8	):	):	PUNCT
mkg-58282	117	9	1577–85	1577–85	NUM
mkg-58282	117	10	.	.	PUNCT
mkg-58282	118	1	2	2	X
mkg-58282	118	2	.	.	X
mkg-58282	118	3	nakamura	nakamura	PROPN
mkg-58282	118	4	s	s	PROPN
mkg-58282	118	5	,	,	PUNCT
mkg-58282	118	6	ito	ito	PROPN
mkg-58282	118	7	t	t	PROPN
mkg-58282	118	8	,	,	PUNCT
mkg-58282	118	9	okamoto	okamoto	PROPN
mkg-58282	118	10	k	k	PROPN
mkg-58282	118	11	,	,	PUNCT
mkg-58282	118	12	mima	mima	PROPN
mkg-58282	118	13	t	t	PROPN
mkg-58282	118	14	,	,	PUNCT
mkg-58282	118	15	uchida	uchida	PROPN
mkg-58282	118	16	k	k	PROPN
mkg-58282	118	17	,	,	PUNCT
mkg-58282	118	18	siddiqui	siddiqui	PROPN
mkg-58282	118	19	yd	yd	PROPN
mkg-58282	118	20	,	,	PUNCT
mkg-58282	118	21	ito	ito	PROPN
mkg-58282	118	22	m	m	PROPN
mkg-58282	118	23	,	,	PUNCT
mkg-58282	118	24	tai	tai	PROPN
mkg-58282	118	25	m	m	PROPN
mkg-58282	118	26	,	,	PUNCT
mkg-58282	118	27	okubo	okubo	PROPN
mkg-58282	118	28	k	k	PROPN
mkg-58282	118	29	,	,	PUNCT
mkg-58282	118	30	yamashiro	yamashiro	PROPN
mkg-58282	118	31	k	k	PROPN
mkg-58282	118	32	,	,	PUNCT
mkg-58282	118	33	omori	omori	PROPN
mkg-58282	118	34	k	k	PROPN
mkg-58282	118	35	,	,	PUNCT
mkg-58282	118	36	yamamoto	yamamoto	PROPN
mkg-58282	118	37	t	t	PROPN
mkg-58282	118	38	,	,	PUNCT
mkg-58282	118	39	matsushita	matsushita	PROPN
mkg-58282	118	40	o	o	PROPN
mkg-58282	118	41	,	,	PUNCT
mkg-58282	118	42	takashiba	takashiba	PROPN
mkg-58282	118	43	s.	s.	PROPN
mkg-58282	118	44	acceleration	acceleration	PROPN
mkg-58282	118	45	of	of	ADP
mkg-58282	118	46	bone	bone	NOUN
mkg-58282	118	47	regeneration	regeneration	NOUN
mkg-58282	118	48	of	of	ADP
mkg-58282	118	49	horizontal	horizontal	ADJ
mkg-58282	118	50	bone	bone	NOUN
mkg-58282	118	51	defect	defect	NOUN
mkg-58282	118	52	in	in	ADP
mkg-58282	118	53	rats	rat	NOUN
mkg-58282	118	54	using	use	VERB
mkg-58282	118	55	collagen	collagen	NOUN
mkg-58282	118	56	-	-	PUNCT
mkg-58282	118	57	binding	bind	VERB
mkg-58282	118	58	basic	basic	ADJ
mkg-58282	118	59	fibroblast	fibroblast	ADJ
mkg-58282	118	60	growth	growth	NOUN
mkg-58282	118	61	factor	factor	NOUN
mkg-58282	118	62	combined	combine	VERB
mkg-58282	118	63	with	with	ADP
mkg-58282	118	64	collagen	collagen	NOUN
mkg-58282	118	65	scaffolds	scaffold	NOUN
mkg-58282	118	66	.	.	PUNCT
mkg-58282	119	1	j	j	PROPN
mkg-58282	119	2	periodontol	periodontol	NOUN
mkg-58282	119	3	.	.	PUNCT
mkg-58282	120	1	2019	2019	NUM
mkg-58282	120	2	;	;	PUNCT
mkg-58282	120	3	90(9	90(9	NUM
mkg-58282	120	4	):	):	PUNCT
mkg-58282	120	5	1043–52	1043–52	NUM
mkg-58282	120	6	.	.	PUNCT
mkg-58282	121	1	copyright	copyright	NOUN
mkg-58282	121	2	©	©	PROPN
mkg-58282	121	3	2025	2025	NUM
mkg-58282	121	4	dental	dental	ADJ
mkg-58282	121	5	journal	journal	NOUN
mkg-58282	121	6	(	(	PUNCT
mkg-58282	121	7	majalah	majalah	PROPN
mkg-58282	121	8	kedokteran	kedokteran	PROPN
mkg-58282	121	9	gigi	gigi	PROPN
mkg-58282	121	10	)	)	PUNCT
mkg-58282	122	1	p	p	X
mkg-58282	122	2	-	-	PUNCT
mkg-58282	122	3	issn	issn	NOUN
mkg-58282	122	4	:	:	PUNCT
mkg-58282	122	5	1978	1978	NUM
mkg-58282	122	6	-	-	SYM
mkg-58282	122	7	3728	3728	NUM
mkg-58282	122	8	;	;	PUNCT
mkg-58282	122	9	e	e	X
mkg-58282	122	10	-	-	PUNCT
mkg-58282	122	11	issn	issn	ADJ
mkg-58282	122	12	:	:	PUNCT
mkg-58282	122	13	2442	2442	NUM
mkg-58282	122	14	-	-	SYM
mkg-58282	122	15	9740	9740	NUM
mkg-58282	122	16	.	.	PUNCT
mkg-58282	123	1	accredited	accredit	VERB
mkg-58282	123	2	no	no	INTJ
mkg-58282	123	3	.	.	PROPN
mkg-58282	124	1	158	158	NUM
mkg-58282	124	2	/	/	SYM
mkg-58282	124	3	e	e	NOUN
mkg-58282	124	4	/	/	SYM
mkg-58282	124	5	kpt/2021	kpt/2021	NOUN
mkg-58282	124	6	.	.	PUNCT
mkg-58282	125	1	open	open	ADJ
mkg-58282	125	2	access	access	NOUN
mkg-58282	125	3	under	under	ADP
mkg-58282	125	4	cc	cc	NOUN
mkg-58282	125	5	-	-	PUNCT
mkg-58282	125	6	by	by	ADP
mkg-58282	125	7	-	-	PUNCT
mkg-58282	125	8	sa	sa	NOUN
mkg-58282	125	9	license	license	NOUN
mkg-58282	125	10	.	.	PUNCT
mkg-58282	126	1	available	available	ADJ
mkg-58282	126	2	at	at	ADP
mkg-58282	126	3	https://e-journal.unair.ac.id/mkg/index	https://e-journal.unair.ac.id/mkg/index	NOUN
mkg-58282	126	4	doi	doi	NOUN
mkg-58282	126	5	:	:	PUNCT
mkg-58282	126	6	10.20473	10.20473	NUM
mkg-58282	126	7	/	/	SYM
mkg-58282	126	8	j.djmkg.v58.i3.p219–223	j.djmkg.v58.i3.p219–223	NOUN
mkg-58282	126	9	https://e-journal.unair.ac.id/mkg/index	https://e-journal.unair.ac.id/mkg/index	NOUN
mkg-58282	126	10	https://doi.org/10.20473/j.djmkg.v58.i3.p219-223	https://doi.org/10.20473/j.djmkg.v58.i3.p219-223	VERB
mkg-58282	126	11	223widyantoro	223widyantoro	PROPN
mkg-58282	126	12	et	et	NOUN
mkg-58282	126	13	al	al	PROPN
mkg-58282	126	14	.	.	PROPN
mkg-58282	126	15	dent	dent	PROPN
mkg-58282	126	16	.	.	PUNCT
mkg-58282	127	1	j.	j.	PROPN
mkg-58282	127	2	(	(	PUNCT
mkg-58282	127	3	majalah	majalah	PROPN
mkg-58282	127	4	kedokteran	kedokteran	PROPN
mkg-58282	127	5	gigi	gigi	PROPN
mkg-58282	127	6	)	)	PUNCT
mkg-58282	127	7	2025	2025	NUM
mkg-58282	127	8	september	september	PROPN
mkg-58282	127	9	;	;	PUNCT
mkg-58282	127	10	58(3	58(3	NUM
mkg-58282	127	11	):	):	PUNCT
mkg-58282	127	12	219–223	219–223	NUM
mkg-58282	127	13	3	3	NUM
mkg-58282	127	14	.	.	PUNCT
mkg-58282	128	1	jeon	jeon	PROPN
mkg-58282	128	2	e	e	PROPN
mkg-58282	128	3	,	,	PUNCT
mkg-58282	128	4	yun	yun	PROPN
mkg-58282	128	5	y	y	PROPN
mkg-58282	128	6	-	-	PUNCT
mkg-58282	128	7	r	r	PROPN
mkg-58282	128	8	,	,	PUNCT
mkg-58282	128	9	kim	kim	PROPN
mkg-58282	128	10	h	h	PROPN
mkg-58282	128	11	-	-	PUNCT
mkg-58282	128	12	w	w	PROPN
mkg-58282	128	13	,	,	PUNCT
mkg-58282	128	14	jang	jang	PROPN
mkg-58282	128	15	j	j	PROPN
mkg-58282	128	16	-	-	PROPN
mkg-58282	128	17	h.	h.	PROPN
mkg-58282	128	18	engineering	engineering	NOUN
mkg-58282	128	19	and	and	CCONJ
mkg-58282	128	20	application	application	NOUN
mkg-58282	128	21	of	of	ADP
mkg-58282	128	22	collagen	collagen	NOUN
mkg-58282	128	23	-	-	PUNCT
mkg-58282	128	24	binding	bind	VERB
mkg-58282	128	25	fibroblast	fibroblast	ADJ
mkg-58282	128	26	growth	growth	NOUN
mkg-58282	128	27	factor	factor	NOUN
mkg-58282	128	28	2	2	NUM
mkg-58282	128	29	for	for	ADP
mkg-58282	128	30	sustained	sustained	ADJ
mkg-58282	128	31	release	release	NOUN
mkg-58282	128	32	.	.	PUNCT
mkg-58282	129	1	j	j	PROPN
mkg-58282	129	2	biomed	biome	VERB
mkg-58282	129	3	mater	mater	NOUN
mkg-58282	129	4	res	re	NOUN
mkg-58282	129	5	part	part	NOUN
mkg-58282	129	6	a.	a.	NOUN
mkg-58282	129	7	2014	2014	NUM
mkg-58282	129	8	;	;	PUNCT
mkg-58282	129	9	102(1	102(1	NUM
mkg-58282	129	10	):	):	PUNCT
mkg-58282	129	11	1–7	1–7	NUM
mkg-58282	129	12	.	.	NOUN
mkg-58282	129	13	4	4	NUM
mkg-58282	129	14	.	.	X
mkg-58282	129	15	paulini	paulini	PROPN
mkg-58282	129	16	m	m	PROPN
mkg-58282	129	17	,	,	PUNCT
mkg-58282	129	18	camal	camal	ADJ
mkg-58282	129	19	ruggieri	ruggieri	NOUN
mkg-58282	129	20	in	in	ADP
mkg-58282	129	21	,	,	PUNCT
mkg-58282	129	22	ramallo	ramallo	PROPN
mkg-58282	129	23	m	m	PROPN
mkg-58282	129	24	,	,	PUNCT
mkg-58282	129	25	alonso	alonso	PROPN
mkg-58282	129	26	m	m	PROPN
mkg-58282	129	27	,	,	PUNCT
mkg-58282	129	28	rodriguezcabello	rodriguezcabello	PROPN
mkg-58282	129	29	jc	jc	PROPN
mkg-58282	129	30	,	,	PUNCT
mkg-58282	129	31	esbrit	esbrit	VERB
mkg-58282	129	32	p	p	NOUN
mkg-58282	129	33	,	,	PUNCT
mkg-58282	129	34	mardegan	mardegan	VERB
mkg-58282	129	35	issa	issa	PROPN
mkg-58282	129	36	jp	jp	PROPN
mkg-58282	129	37	,	,	PUNCT
mkg-58282	129	38	feldman	feldman	PROPN
mkg-58282	129	39	s.	s.	PROPN
mkg-58282	129	40	recombinant	recombinant	ADJ
mkg-58282	129	41	proteins	protein	NOUN
mkg-58282	129	42	-	-	PUNCT
mkg-58282	129	43	based	base	VERB
mkg-58282	129	44	strategies	strategy	NOUN
mkg-58282	129	45	in	in	ADP
mkg-58282	129	46	bone	bone	NOUN
mkg-58282	129	47	tissue	tissue	NOUN
mkg-58282	129	48	engineering	engineering	NOUN
mkg-58282	129	49	.	.	PUNCT
mkg-58282	130	1	biomolecules	biomolecule	NOUN
mkg-58282	130	2	.	.	PUNCT
mkg-58282	130	3	2021	2021	NUM
mkg-58282	130	4	;	;	PUNCT
mkg-58282	130	5	12(1	12(1	NUM
mkg-58282	130	6	):	):	PUNCT
mkg-58282	130	7	3	3	NUM
mkg-58282	130	8	.	.	NOUN
mkg-58282	130	9	5	5	NUM
mkg-58282	130	10	.	.	X
mkg-58282	130	11	abe	abe	PROPN
mkg-58282	130	12	f	f	PROPN
mkg-58282	130	13	,	,	PUNCT
mkg-58282	130	14	nakano	nakano	PROPN
mkg-58282	130	15	a	a	X
mkg-58282	130	16	,	,	PUNCT
mkg-58282	130	17	hirata	hirata	NOUN
mkg-58282	130	18	i	i	PROPN
mkg-58282	130	19	,	,	PUNCT
mkg-58282	130	20	tanimoto	tanimoto	PROPN
mkg-58282	130	21	k	k	PROPN
mkg-58282	130	22	,	,	PUNCT
mkg-58282	130	23	kato	kato	PROPN
mkg-58282	130	24	k.	k.	PROPN
mkg-58282	130	25	structure	structure	PROPN
mkg-58282	130	26	and	and	CCONJ
mkg-58282	130	27	function	function	NOUN
mkg-58282	130	28	of	of	ADP
mkg-58282	130	29	engineered	engineer	VERB
mkg-58282	130	30	stromal	stromal	ADJ
mkg-58282	130	31	cell	cell	NOUN
mkg-58282	130	32	-	-	PUNCT
mkg-58282	130	33	derived	derive	VERB
mkg-58282	130	34	factor-1α	factor-1α	NOUN
mkg-58282	130	35	.	.	PUNCT
mkg-58282	131	1	dent	dent	NOUN
mkg-58282	131	2	mater	mater	NOUN
mkg-58282	131	3	j.	j.	PROPN
mkg-58282	131	4	2024	2024	NUM
mkg-58282	131	5	;	;	PUNCT
mkg-58282	131	6	43(2	43(2	NUM
mkg-58282	131	7	):	):	PUNCT
mkg-58282	131	8	2023–247	2023–247	NOUN
mkg-58282	131	9	.	.	PUNCT
mkg-58282	132	1	6	6	X
mkg-58282	132	2	.	.	X
mkg-58282	132	3	shakya	shakya	PROPN
mkg-58282	132	4	a	a	PROPN
mkg-58282	132	5	,	,	PUNCT
mkg-58282	132	6	imado	imado	ADJ
mkg-58282	132	7	e	e	NOUN
mkg-58282	132	8	,	,	PUNCT
mkg-58282	132	9	nguyen	nguyen	PROPN
mkg-58282	132	10	pk	pk	PROPN
mkg-58282	132	11	,	,	PUNCT
mkg-58282	132	12	matsuyama	matsuyama	PROPN
mkg-58282	132	13	t	t	PROPN
mkg-58282	132	14	,	,	PUNCT
mkg-58282	132	15	horimoto	horimoto	PROPN
mkg-58282	132	16	k	k	PROPN
mkg-58282	132	17	,	,	PUNCT
mkg-58282	132	18	hirata	hirata	VERB
mkg-58282	132	19	i	i	PROPN
mkg-58282	132	20	,	,	PUNCT
mkg-58282	132	21	kato	kato	PROPN
mkg-58282	132	22	k.	k.	PROPN
mkg-58282	132	23	oriented	orient	VERB
mkg-58282	132	24	immobilization	immobilization	NOUN
mkg-58282	132	25	of	of	ADP
mkg-58282	132	26	basic	basic	ADJ
mkg-58282	132	27	fibroblast	fibroblast	ADJ
mkg-58282	132	28	growth	growth	NOUN
mkg-58282	132	29	factor	factor	NOUN
mkg-58282	132	30	:	:	PUNCT
mkg-58282	132	31	bioengineered	bioengineere	VERB
mkg-58282	132	32	surface	surface	NOUN
mkg-58282	132	33	design	design	NOUN
mkg-58282	132	34	for	for	ADP
mkg-58282	132	35	the	the	DET
mkg-58282	132	36	expansion	expansion	NOUN
mkg-58282	132	37	of	of	ADP
mkg-58282	132	38	human	human	ADJ
mkg-58282	132	39	mesenchymal	mesenchymal	ADJ
mkg-58282	132	40	stromal	stromal	ADJ
mkg-58282	132	41	cells	cell	NOUN
mkg-58282	132	42	.	.	PUNCT
mkg-58282	133	1	sci	sci	PROPN
mkg-58282	133	2	rep	rep	PROPN
mkg-58282	133	3	.	.	PROPN
mkg-58282	133	4	2020	2020	NUM
mkg-58282	133	5	;	;	PUNCT
mkg-58282	133	6	10(1	10(1	NUM
mkg-58282	133	7	):	):	PUNCT
mkg-58282	133	8	8762	8762	NUM
mkg-58282	133	9	.	.	PUNCT
mkg-58282	134	1	7	7	X
mkg-58282	134	2	.	.	X
mkg-58282	134	3	nakano	nakano	PROPN
mkg-58282	134	4	a	a	PROPN
mkg-58282	134	5	,	,	PUNCT
mkg-58282	134	6	kato	kato	PROPN
mkg-58282	134	7	k.	k.	PROPN
mkg-58282	134	8	regenerative	regenerative	PROPN
mkg-58282	134	9	nanotechnology	nanotechnology	PROPN
mkg-58282	134	10	:	:	PUNCT
mkg-58282	134	11	engineered	engineer	VERB
mkg-58282	134	12	surfaces	surface	NOUN
mkg-58282	134	13	for	for	ADP
mkg-58282	134	14	stem	stem	NOUN
mkg-58282	134	15	cell	cell	NOUN
mkg-58282	134	16	production	production	NOUN
mkg-58282	134	17	.	.	PUNCT
mkg-58282	135	1	in	in	ADP
mkg-58282	135	2	:	:	PUNCT
mkg-58282	135	3	gu	gu	NOUN
mkg-58282	135	4	n	n	NOUN
mkg-58282	135	5	,	,	PUNCT
mkg-58282	135	6	editor	editor	NOUN
mkg-58282	135	7	.	.	PUNCT
mkg-58282	136	1	nanomedicine	nanomedicine	PROPN
mkg-58282	136	2	micro	micro	PROPN
mkg-58282	136	3	/	/	PROPN
mkg-58282	136	4	nano	nano	NOUN
mkg-58282	136	5	technologies	technology	NOUN
mkg-58282	136	6	.	.	PUNCT
mkg-58282	137	1	springer	springer	NOUN
mkg-58282	137	2	;	;	PUNCT
mkg-58282	137	3	2023	2023	NUM
mkg-58282	137	4	.	.	PUNCT
mkg-58282	138	1	p.	p.	NOUN
mkg-58282	138	2	605–22	605–22	PROPN
mkg-58282	138	3	.	.	PUNCT
mkg-58282	139	1	8	8	NUM
mkg-58282	139	2	.	.	PUNCT
mkg-58282	140	1	beygmoradi	beygmoradi	PROPN
mkg-58282	140	2	a	a	PRON
mkg-58282	140	3	,	,	PUNCT
mkg-58282	140	4	homaei	homaei	NOUN
mkg-58282	140	5	a	a	X
mkg-58282	140	6	,	,	PUNCT
mkg-58282	140	7	hemmati	hemmati	PROPN
mkg-58282	140	8	r	r	PROPN
mkg-58282	140	9	,	,	PUNCT
mkg-58282	140	10	fernandes	fernande	NOUN
mkg-58282	140	11	p.	p.	NOUN
mkg-58282	140	12	recombinant	recombinant	ADJ
mkg-58282	140	13	protein	protein	NOUN
mkg-58282	140	14	expression	expression	NOUN
mkg-58282	140	15	:	:	PUNCT
mkg-58282	140	16	challenges	challenge	NOUN
mkg-58282	140	17	in	in	ADP
mkg-58282	140	18	production	production	NOUN
mkg-58282	140	19	and	and	CCONJ
mkg-58282	140	20	folding	fold	VERB
mkg-58282	140	21	related	related	ADJ
mkg-58282	140	22	matters	matter	NOUN
mkg-58282	140	23	.	.	PUNCT
mkg-58282	141	1	int	int	NOUN
mkg-58282	141	2	j	j	PROPN
mkg-58282	141	3	biol	biol	PROPN
mkg-58282	141	4	macromol	macromol	PROPN
mkg-58282	141	5	.	.	PUNCT
mkg-58282	142	1	2023	2023	NUM
mkg-58282	142	2	;	;	PUNCT
mkg-58282	142	3	233	233	NUM
mkg-58282	142	4	:	:	SYM
mkg-58282	142	5	123407	123407	NUM
mkg-58282	142	6	.	.	PUNCT
mkg-58282	143	1	9	9	X
mkg-58282	143	2	.	.	X
mkg-58282	143	3	yamaguchi	yamaguchi	PROPN
mkg-58282	143	4	h	h	PROPN
mkg-58282	143	5	,	,	PUNCT
mkg-58282	143	6	miyazaki	miyazaki	PROPN
mkg-58282	143	7	m.	m.	NOUN
mkg-58282	143	8	refolding	refolde	VERB
mkg-58282	143	9	techniques	technique	NOUN
mkg-58282	143	10	for	for	ADP
mkg-58282	143	11	recovering	recover	VERB
mkg-58282	143	12	biologically	biologically	ADV
mkg-58282	143	13	active	active	ADJ
mkg-58282	143	14	recombinant	recombinant	ADJ
mkg-58282	143	15	proteins	protein	NOUN
mkg-58282	143	16	from	from	ADP
mkg-58282	143	17	inclusion	inclusion	NOUN
mkg-58282	143	18	bodies	body	NOUN
mkg-58282	143	19	.	.	PUNCT
mkg-58282	144	1	biomolecules	biomolecule	NOUN
mkg-58282	144	2	.	.	PUNCT
mkg-58282	145	1	2014	2014	NUM
mkg-58282	145	2	;	;	PUNCT
mkg-58282	146	1	4(1	4(1	NOUN
mkg-58282	146	2	):	):	PUNCT
mkg-58282	146	3	235–51	235–51	NUM
mkg-58282	146	4	.	.	X
mkg-58282	147	1	10	10	NUM
mkg-58282	147	2	.	.	PUNCT
mkg-58282	148	1	wang	wang	PROPN
mkg-58282	148	2	w	w	PROPN
mkg-58282	148	3	,	,	PUNCT
mkg-58282	148	4	roberts	roberts	PROPN
mkg-58282	148	5	cj	cj	PROPN
mkg-58282	148	6	.	.	PUNCT
mkg-58282	148	7	protein	protein	NOUN
mkg-58282	148	8	aggregation	aggregation	NOUN
mkg-58282	148	9	–	–	PUNCT
mkg-58282	148	10	mechanisms	mechanism	NOUN
mkg-58282	148	11	,	,	PUNCT
mkg-58282	148	12	detection	detection	NOUN
mkg-58282	148	13	,	,	PUNCT
mkg-58282	148	14	and	and	CCONJ
mkg-58282	148	15	control	control	NOUN
mkg-58282	148	16	.	.	PUNCT
mkg-58282	149	1	int	int	PROPN
mkg-58282	149	2	j	j	PROPN
mkg-58282	149	3	pharm	pharm	PROPN
mkg-58282	149	4	.	.	PUNCT
mkg-58282	150	1	2018	2018	NUM
mkg-58282	150	2	;	;	PUNCT
mkg-58282	150	3	550(1–2	550(1–2	NUM
mkg-58282	150	4	):	):	PUNCT
mkg-58282	150	5	251–68	251–68	NUM
mkg-58282	150	6	.	.	PUNCT
mkg-58282	151	1	11	11	NUM
mkg-58282	151	2	.	.	PUNCT
mkg-58282	152	1	shendge	shendge	PROPN
mkg-58282	152	2	aa	aa	PROPN
mkg-58282	152	3	,	,	PUNCT
mkg-58282	152	4	d’souza	d’souza	PROPN
mkg-58282	152	5	js	js	PROPN
mkg-58282	152	6	.	.	PUNCT
mkg-58282	153	1	strategic	strategic	ADJ
mkg-58282	153	2	optimization	optimization	NOUN
mkg-58282	153	3	of	of	ADP
mkg-58282	153	4	conditions	condition	NOUN
mkg-58282	153	5	for	for	ADP
mkg-58282	153	6	the	the	DET
mkg-58282	153	7	solubilization	solubilization	NOUN
mkg-58282	153	8	of	of	ADP
mkg-58282	153	9	gst	gst	NOUN
mkg-58282	153	10	-	-	PUNCT
mkg-58282	153	11	tagged	tag	VERB
mkg-58282	153	12	amphipathic	amphipathic	ADJ
mkg-58282	153	13	helix	helix	NOUN
mkg-58282	153	14	-	-	PUNCT
mkg-58282	153	15	containing	contain	VERB
mkg-58282	153	16	ciliary	ciliary	ADJ
mkg-58282	153	17	proteins	protein	NOUN
mkg-58282	153	18	overexpressed	overexpresse	VERB
mkg-58282	153	19	as	as	ADP
mkg-58282	153	20	inclusion	inclusion	NOUN
mkg-58282	153	21	bodies	body	NOUN
mkg-58282	153	22	in	in	ADP
mkg-58282	153	23	e.	e.	PROPN
mkg-58282	153	24	coli	coli	PROPN
mkg-58282	153	25	.	.	PUNCT
mkg-58282	154	1	microb	microb	PROPN
mkg-58282	154	2	cell	cell	PROPN
mkg-58282	154	3	fact	fact	NOUN
mkg-58282	154	4	.	.	PUNCT
mkg-58282	155	1	2022	2022	NUM
mkg-58282	155	2	;	;	PUNCT
mkg-58282	155	3	21(1	21(1	NUM
mkg-58282	155	4	):	):	PUNCT
mkg-58282	155	5	258	258	NUM
mkg-58282	155	6	.	.	PUNCT
mkg-58282	155	7	12	12	NUM
mkg-58282	155	8	.	.	PUNCT
mkg-58282	156	1	schäfer	schäfer	NOUN
mkg-58282	156	2	f	f	PROPN
mkg-58282	156	3	,	,	PUNCT
mkg-58282	156	4	seip	seip	NOUN
mkg-58282	156	5	n	n	CCONJ
mkg-58282	156	6	,	,	PUNCT
mkg-58282	156	7	maertens	maertens	PROPN
mkg-58282	156	8	b	b	PROPN
mkg-58282	156	9	,	,	PUNCT
mkg-58282	156	10	block	block	NOUN
mkg-58282	156	11	h	h	NOUN
mkg-58282	156	12	,	,	PUNCT
mkg-58282	156	13	kubicek	kubicek	PROPN
mkg-58282	156	14	j.	j.	PROPN
mkg-58282	156	15	purification	purification	PROPN
mkg-58282	156	16	of	of	ADP
mkg-58282	156	17	gst	gst	NOUN
mkg-58282	156	18	-	-	PUNCT
mkg-58282	156	19	tagged	tag	VERB
mkg-58282	156	20	proteins	protein	NOUN
mkg-58282	156	21	.	.	PUNCT
mkg-58282	157	1	methods	method	NOUN
mkg-58282	157	2	enzymol	enzymol	PROPN
mkg-58282	157	3	.	.	PUNCT
mkg-58282	158	1	2015	2015	NUM
mkg-58282	158	2	;	;	PUNCT
mkg-58282	158	3	559	559	NUM
mkg-58282	158	4	:	:	PUNCT
mkg-58282	158	5	127–39	127–39	NUM
mkg-58282	158	6	.	.	PUNCT
mkg-58282	159	1	13	13	NUM
mkg-58282	159	2	.	.	X
mkg-58282	159	3	moghadam	moghadam	PROPN
mkg-58282	159	4	m	m	PROPN
mkg-58282	159	5	,	,	PUNCT
mkg-58282	159	6	ganji	ganji	X
mkg-58282	159	7	a	a	PRON
mkg-58282	159	8	,	,	PUNCT
mkg-58282	159	9	varasteh	varasteh	NOUN
mkg-58282	159	10	a	a	NOUN
mkg-58282	159	11	,	,	PUNCT
mkg-58282	159	12	falak	falak	ADJ
mkg-58282	159	13	r	r	NOUN
mkg-58282	159	14	,	,	PUNCT
mkg-58282	159	15	sankian	sankian	ADJ
mkg-58282	159	16	m.	m.	NOUN
mkg-58282	159	17	refolding	refolding	NOUN
mkg-58282	159	18	process	process	NOUN
mkg-58282	159	19	of	of	ADP
mkg-58282	159	20	cysteine	cysteine	NOUN
mkg-58282	159	21	-	-	PUNCT
mkg-58282	159	22	rich	rich	ADJ
mkg-58282	159	23	proteins	protein	NOUN
mkg-58282	159	24	:	:	PUNCT
mkg-58282	159	25	chitinase	chitinase	NOUN
mkg-58282	159	26	as	as	ADP
mkg-58282	159	27	a	a	DET
mkg-58282	159	28	model	model	NOUN
mkg-58282	159	29	.	.	PUNCT
mkg-58282	160	1	reports	report	VERB
mkg-58282	160	2	biochem	biochem	PROPN
mkg-58282	160	3	mol	mol	ADP
mkg-58282	160	4	biol	biol	PROPN
mkg-58282	160	5	.	.	PUNCT
mkg-58282	161	1	2015	2015	NUM
mkg-58282	161	2	;	;	PUNCT
mkg-58282	161	3	4(1	4(1	NOUN
mkg-58282	161	4	):	):	PUNCT
mkg-58282	161	5	19–24	19–24	NUM
mkg-58282	161	6	.	.	PUNCT
mkg-58282	162	1	14	14	NUM
mkg-58282	162	2	.	.	PUNCT
mkg-58282	163	1	li	li	PROPN
mkg-58282	163	2	x	x	PROPN
mkg-58282	163	3	,	,	PUNCT
mkg-58282	163	4	jin	jin	PROPN
mkg-58282	163	5	j	j	PROPN
mkg-58282	163	6	,	,	PUNCT
mkg-58282	163	7	guo	guo	PROPN
mkg-58282	163	8	z	z	PROPN
mkg-58282	163	9	,	,	PUNCT
mkg-58282	163	10	liu	liu	PROPN
mkg-58282	163	11	l.	l.	PROPN
mkg-58282	163	12	evolution	evolution	PROPN
mkg-58282	163	13	of	of	ADP
mkg-58282	163	14	plasmid	plasmid	NOUN
mkg-58282	163	15	-	-	PUNCT
mkg-58282	163	16	construction	construction	NOUN
mkg-58282	163	17	.	.	PUNCT
mkg-58282	164	1	int	int	NOUN
mkg-58282	164	2	j	j	PROPN
mkg-58282	164	3	biol	biol	PROPN
mkg-58282	164	4	macromol	macromol	PROPN
mkg-58282	164	5	.	.	PUNCT
mkg-58282	165	1	2022	2022	NUM
mkg-58282	165	2	;	;	PUNCT
mkg-58282	165	3	209(pt	209(pt	NUM
mkg-58282	165	4	a	a	NOUN
mkg-58282	165	5	):	):	PUNCT
mkg-58282	165	6	1319–26	1319–26	NOUN
mkg-58282	165	7	.	.	PUNCT
mkg-58282	166	1	15	15	NUM
mkg-58282	166	2	.	.	PUNCT
mkg-58282	166	3	konagaya	konagaya	PROPN
mkg-58282	166	4	s	s	PROPN
mkg-58282	166	5	,	,	PUNCT
mkg-58282	166	6	kato	kato	PROPN
mkg-58282	166	7	k	k	PROPN
mkg-58282	166	8	,	,	PUNCT
mkg-58282	166	9	nakaji	nakaji	PROPN
mkg-58282	166	10	-	-	PUNCT
mkg-58282	166	11	hirabayashi	hirabayashi	PROPN
mkg-58282	166	12	t	t	PROPN
mkg-58282	166	13	,	,	PUNCT
mkg-58282	166	14	arima	arima	PROPN
mkg-58282	166	15	y	y	PROPN
mkg-58282	166	16	,	,	PUNCT
mkg-58282	166	17	iwata	iwata	PROPN
mkg-58282	166	18	h.	h.	PROPN
mkg-58282	166	19	array	array	PROPN
mkg-58282	166	20	-	-	PUNCT
mkg-58282	166	21	based	base	VERB
mkg-58282	166	22	functional	functional	ADJ
mkg-58282	166	23	screening	screening	NOUN
mkg-58282	166	24	of	of	ADP
mkg-58282	166	25	growth	growth	NOUN
mkg-58282	166	26	factors	factor	NOUN
mkg-58282	166	27	toward	toward	ADP
mkg-58282	166	28	optimizing	optimize	VERB
mkg-58282	166	29	neural	neural	ADJ
mkg-58282	166	30	stem	stem	NOUN
mkg-58282	166	31	cell	cell	NOUN
mkg-58282	166	32	microenvironments	microenvironment	NOUN
mkg-58282	166	33	.	.	PUNCT
mkg-58282	166	34	biomaterials	biomaterial	NOUN
mkg-58282	166	35	.	.	PUNCT
mkg-58282	167	1	2011	2011	NUM
mkg-58282	167	2	;	;	PUNCT
mkg-58282	168	1	32(22	32(22	NUM
mkg-58282	168	2	):	):	PUNCT
mkg-58282	168	3	5015–22	5015–22	NOUN
mkg-58282	168	4	.	.	PUNCT
mkg-58282	169	1	16	16	NUM
mkg-58282	169	2	.	.	PUNCT
mkg-58282	169	3	drew	draw	VERB
mkg-58282	169	4	ed	ed	NOUN
mkg-58282	169	5	,	,	PUNCT
mkg-58282	169	6	janes	janes	PROPN
mkg-58282	169	7	rw	rw	PROPN
mkg-58282	169	8	.	.	PUNCT
mkg-58282	170	1	pdbmd2cd	pdbmd2cd	NOUN
mkg-58282	170	2	:	:	PUNCT
mkg-58282	170	3	providing	provide	VERB
mkg-58282	170	4	predicted	predict	VERB
mkg-58282	170	5	protein	protein	NOUN
mkg-58282	170	6	circular	circular	ADJ
mkg-58282	170	7	dichroism	dichroism	NOUN
mkg-58282	170	8	spectra	spectra	NOUN
mkg-58282	170	9	from	from	ADP
mkg-58282	170	10	multiple	multiple	ADJ
mkg-58282	170	11	molecular	molecular	ADJ
mkg-58282	170	12	dynamicsgenerated	dynamicsgenerate	VERB
mkg-58282	170	13	protein	protein	NOUN
mkg-58282	170	14	structures	structure	NOUN
mkg-58282	170	15	.	.	PUNCT
mkg-58282	171	1	nucleic	nucleic	ADJ
mkg-58282	171	2	acids	acid	NOUN
mkg-58282	171	3	res	re	NOUN
mkg-58282	171	4	.	.	PUNCT
mkg-58282	171	5	2020	2020	NUM
mkg-58282	171	6	;	;	PUNCT
mkg-58282	171	7	48(w1	48(w1	NUM
mkg-58282	171	8	):	):	PUNCT
mkg-58282	171	9	w17–24	w17–24	PROPN
mkg-58282	171	10	.	.	PROPN
mkg-58282	171	11	17	17	NUM
mkg-58282	171	12	.	.	PUNCT
mkg-58282	172	1	jacinto	jacinto	NOUN
mkg-58282	172	2	-	-	PUNCT
mkg-58282	172	3	méndez	méndez	PROPN
mkg-58282	172	4	d	d	PROPN
mkg-58282	172	5	,	,	PUNCT
mkg-58282	172	6	granados	granados	PROPN
mkg-58282	172	7	-	-	PUNCT
mkg-58282	172	8	ramírez	ramírez	PROPN
mkg-58282	172	9	cg	cg	NOUN
mkg-58282	172	10	,	,	PUNCT
mkg-58282	172	11	carbajal	carbajal	NOUN
mkg-58282	172	12	-	-	PUNCT
mkg-58282	172	13	tinoco	tinoco	PROPN
mkg-58282	172	14	md	md	PROPN
mkg-58282	172	15	.	.	PUNCT
mkg-58282	173	1	kcd	kcd	PROPN
mkg-58282	173	2	:	:	PUNCT
mkg-58282	173	3	a	a	DET
mkg-58282	173	4	prediction	prediction	NOUN
mkg-58282	173	5	web	web	NOUN
mkg-58282	173	6	server	server	NOUN
mkg-58282	173	7	of	of	ADP
mkg-58282	173	8	knowledge	knowledge	NOUN
mkg-58282	173	9	-	-	PUNCT
mkg-58282	173	10	based	base	VERB
mkg-58282	173	11	circular	circular	ADJ
mkg-58282	173	12	dichroism	dichroism	NOUN
mkg-58282	173	13	.	.	PUNCT
mkg-58282	174	1	protein	protein	NOUN
mkg-58282	174	2	sci	sci	PROPN
mkg-58282	174	3	.	.	PROPN
mkg-58282	174	4	2024	2024	NUM
mkg-58282	174	5	;	;	PUNCT
mkg-58282	174	6	33(4	33(4	NUM
mkg-58282	174	7	):	):	PUNCT
mkg-58282	174	8	e4967	e4967	PROPN
mkg-58282	174	9	.	.	PROPN
mkg-58282	175	1	18	18	NUM
mkg-58282	175	2	.	.	PUNCT
mkg-58282	176	1	tunyasuvunakool	tunyasuvunakool	VERB
mkg-58282	176	2	k	k	PROPN
mkg-58282	176	3	,	,	PUNCT
mkg-58282	176	4	adler	adler	PROPN
mkg-58282	176	5	j	j	PROPN
mkg-58282	176	6	,	,	PUNCT
mkg-58282	176	7	wu	wu	PROPN
mkg-58282	177	1	z	z	PROPN
mkg-58282	177	2	,	,	PUNCT
mkg-58282	177	3	green	green	PROPN
mkg-58282	177	4	t	t	PROPN
mkg-58282	177	5	,	,	PUNCT
mkg-58282	177	6	zielinski	zielinski	PROPN
mkg-58282	177	7	m	m	PROPN
mkg-58282	177	8	,	,	PUNCT
mkg-58282	177	9	žídek	žídek	PROPN
mkg-58282	177	10	a	a	PROPN
mkg-58282	177	11	,	,	PUNCT
mkg-58282	177	12	bridgland	bridgland	PROPN
mkg-58282	177	13	a	a	PRON
mkg-58282	177	14	,	,	PUNCT
mkg-58282	177	15	cowie	cowie	PROPN
mkg-58282	177	16	a	a	PROPN
mkg-58282	177	17	,	,	PUNCT
mkg-58282	177	18	meyer	meyer	PROPN
mkg-58282	177	19	c	c	PROPN
mkg-58282	177	20	,	,	PUNCT
mkg-58282	177	21	laydon	laydon	VERB
mkg-58282	177	22	a	a	PRON
mkg-58282	177	23	,	,	PUNCT
mkg-58282	177	24	velankar	velankar	PROPN
mkg-58282	177	25	s	s	PROPN
mkg-58282	177	26	,	,	PUNCT
mkg-58282	177	27	kleywegt	kleywegt	PROPN
mkg-58282	177	28	gj	gj	PROPN
mkg-58282	177	29	,	,	PUNCT
mkg-58282	177	30	bateman	bateman	PROPN
mkg-58282	177	31	a	a	PROPN
mkg-58282	177	32	,	,	PUNCT
mkg-58282	177	33	evans	evans	PROPN
mkg-58282	177	34	r	r	PROPN
mkg-58282	177	35	,	,	PUNCT
mkg-58282	177	36	pritzel	pritzel	NOUN
mkg-58282	177	37	a	a	X
mkg-58282	177	38	,	,	PUNCT
mkg-58282	177	39	figurnov	figurnov	PROPN
mkg-58282	177	40	m	m	PROPN
mkg-58282	177	41	,	,	PUNCT
mkg-58282	177	42	ronneberger	ronneberger	NOUN
mkg-58282	177	43	o	o	NOUN
mkg-58282	177	44	,	,	PUNCT
mkg-58282	177	45	bates	bates	PROPN
mkg-58282	177	46	r	r	NOUN
mkg-58282	177	47	,	,	PUNCT
mkg-58282	177	48	kohl	kohl	NOUN
mkg-58282	177	49	saa	saa	PROPN
mkg-58282	177	50	,	,	PUNCT
mkg-58282	177	51	potapenko	potapenko	PROPN
mkg-58282	177	52	a	a	PRON
mkg-58282	177	53	,	,	PUNCT
mkg-58282	177	54	ballard	ballard	PROPN
mkg-58282	177	55	aj	aj	PROPN
mkg-58282	177	56	,	,	PUNCT
mkg-58282	177	57	romera	romera	NOUN
mkg-58282	177	58	-	-	PUNCT
mkg-58282	177	59	paredes	paredes	PROPN
mkg-58282	177	60	b	b	NOUN
mkg-58282	177	61	,	,	PUNCT
mkg-58282	177	62	nikolov	nikolov	PROPN
mkg-58282	177	63	s	s	PROPN
mkg-58282	177	64	,	,	PUNCT
mkg-58282	177	65	jain	jain	PROPN
mkg-58282	177	66	r	r	NOUN
mkg-58282	177	67	,	,	PUNCT
mkg-58282	177	68	clancy	clancy	NOUN
mkg-58282	177	69	e	e	NOUN
mkg-58282	177	70	,	,	PUNCT
mkg-58282	177	71	reiman	reiman	NOUN
mkg-58282	177	72	d	d	PROPN
mkg-58282	177	73	,	,	PUNCT
mkg-58282	177	74	petersen	petersen	PROPN
mkg-58282	177	75	s	s	PROPN
mkg-58282	177	76	,	,	PUNCT
mkg-58282	177	77	senior	senior	ADJ
mkg-58282	177	78	aw	aw	INTJ
mkg-58282	177	79	,	,	PUNCT
mkg-58282	177	80	kavukcuoglu	kavukcuoglu	PROPN
mkg-58282	177	81	k	k	PROPN
mkg-58282	177	82	,	,	PUNCT
mkg-58282	177	83	birney	birney	PROPN
mkg-58282	177	84	e	e	PROPN
mkg-58282	177	85	,	,	PUNCT
mkg-58282	177	86	kohli	kohli	PROPN
mkg-58282	177	87	p	p	PROPN
mkg-58282	177	88	,	,	PUNCT
mkg-58282	177	89	jumper	jumper	PROPN
mkg-58282	177	90	j	j	PROPN
mkg-58282	177	91	,	,	PUNCT
mkg-58282	177	92	hassabis	hassabis	PRON
mkg-58282	178	1	d.	d.	NOUN
mkg-58282	178	2	highly	highly	ADV
mkg-58282	178	3	accurate	accurate	ADJ
mkg-58282	178	4	protein	protein	NOUN
mkg-58282	178	5	structure	structure	NOUN
mkg-58282	178	6	prediction	prediction	NOUN
mkg-58282	178	7	for	for	ADP
mkg-58282	178	8	the	the	DET
mkg-58282	178	9	human	human	ADJ
mkg-58282	178	10	proteome	proteome	PROPN
mkg-58282	178	11	.	.	PUNCT
mkg-58282	179	1	nature	nature	NOUN
mkg-58282	179	2	.	.	PUNCT
mkg-58282	180	1	2021	2021	NUM
mkg-58282	180	2	;	;	PUNCT
mkg-58282	180	3	596(7873	596(7873	NUM
mkg-58282	180	4	):	):	PUNCT
mkg-58282	180	5	590–6	590–6	NUM
mkg-58282	180	6	.	.	PROPN
mkg-58282	180	7	19	19	NUM
mkg-58282	180	8	.	.	PUNCT
mkg-58282	181	1	evans	evans	PROPN
mkg-58282	181	2	r	r	PROPN
mkg-58282	181	3	,	,	PUNCT
mkg-58282	181	4	o’neill	o’neill	NOUN
mkg-58282	181	5	m	m	PROPN
mkg-58282	181	6	,	,	PUNCT
mkg-58282	181	7	pritzel	pritzel	VERB
mkg-58282	181	8	a	a	PRON
mkg-58282	181	9	,	,	PUNCT
mkg-58282	181	10	antropova	antropova	PROPN
mkg-58282	181	11	n	n	CCONJ
mkg-58282	181	12	,	,	PUNCT
mkg-58282	181	13	senior	senior	ADJ
mkg-58282	181	14	a	a	DET
mkg-58282	181	15	,	,	PUNCT
mkg-58282	181	16	green	green	PROPN
mkg-58282	181	17	t	t	PROPN
mkg-58282	181	18	,	,	PUNCT
mkg-58282	181	19	žídek	žídek	PROPN
mkg-58282	182	1	a	a	PROPN
mkg-58282	182	2	,	,	PUNCT
mkg-58282	182	3	bates	bates	PROPN
mkg-58282	182	4	r	r	PROPN
mkg-58282	182	5	,	,	PUNCT
mkg-58282	182	6	blackwell	blackwell	PROPN
mkg-58282	182	7	s	s	PROPN
mkg-58282	182	8	,	,	PUNCT
mkg-58282	182	9	yim	yim	PROPN
mkg-58282	182	10	j	j	PROPN
mkg-58282	182	11	,	,	PUNCT
mkg-58282	182	12	ronneberger	ronneberger	NOUN
mkg-58282	182	13	o	o	PROPN
mkg-58282	182	14	,	,	PUNCT
mkg-58282	182	15	bodenstein	bodenstein	PROPN
mkg-58282	182	16	s	s	PROPN
mkg-58282	182	17	,	,	PUNCT
mkg-58282	182	18	zielinski	zielinski	PROPN
mkg-58282	182	19	m	m	PROPN
mkg-58282	182	20	,	,	PUNCT
mkg-58282	182	21	bridgland	bridgland	X
mkg-58282	182	22	a	a	PRON
mkg-58282	182	23	,	,	PUNCT
mkg-58282	182	24	potapenko	potapenko	PROPN
mkg-58282	182	25	a	a	PRON
mkg-58282	182	26	,	,	PUNCT
mkg-58282	182	27	cowie	cowie	PROPN
mkg-58282	182	28	a	a	PROPN
mkg-58282	182	29	,	,	PUNCT
mkg-58282	182	30	tunyasuvunakool	tunyasuvunakool	VERB
mkg-58282	182	31	k	k	PROPN
mkg-58282	182	32	,	,	PUNCT
mkg-58282	182	33	jain	jain	PROPN
mkg-58282	182	34	r	r	PROPN
mkg-58282	182	35	,	,	PUNCT
mkg-58282	182	36	clancy	clancy	NOUN
mkg-58282	182	37	e	e	PROPN
mkg-58282	182	38	,	,	PUNCT
mkg-58282	182	39	kohli	kohli	PROPN
mkg-58282	182	40	p	p	PROPN
mkg-58282	182	41	,	,	PUNCT
mkg-58282	182	42	jumper	jumper	PROPN
mkg-58282	182	43	j	j	PROPN
mkg-58282	182	44	,	,	PUNCT
mkg-58282	182	45	hassabis	hassabis	PRON
mkg-58282	182	46	d.	d.	NOUN
mkg-58282	182	47	protein	protein	NOUN
mkg-58282	182	48	complex	complex	ADJ
mkg-58282	182	49	prediction	prediction	NOUN
mkg-58282	182	50	with	with	ADP
mkg-58282	182	51	alphafold	alphafold	ADJ
mkg-58282	182	52	-	-	PUNCT
mkg-58282	182	53	multimer	multimer	NOUN
mkg-58282	182	54	.	.	PROPN
mkg-58282	182	55	biorxiv	biorxiv	PROPN
mkg-58282	182	56	.	.	PUNCT
mkg-58282	183	1	2022	2022	NUM
mkg-58282	183	2	.	.	PUNCT
mkg-58282	184	1	p.	p.	NOUN
mkg-58282	184	2	1–25	1–25	PROPN
mkg-58282	184	3	.	.	PUNCT
mkg-58282	185	1	20	20	NUM
mkg-58282	185	2	.	.	PUNCT
mkg-58282	186	1	yamauchi	yamauchi	PROPN
mkg-58282	186	2	y	y	PROPN
mkg-58282	186	3	,	,	PUNCT
mkg-58282	186	4	hirata	hirata	VERB
mkg-58282	186	5	i	i	PROPN
mkg-58282	186	6	,	,	PUNCT
mkg-58282	186	7	tanimoto	tanimoto	PROPN
mkg-58282	186	8	k	k	PROPN
mkg-58282	186	9	,	,	PUNCT
mkg-58282	186	10	kato	kato	PROPN
mkg-58282	186	11	k.	k.	PROPN
mkg-58282	186	12	epidermal	epidermal	ADJ
mkg-58282	186	13	growth	growth	NOUN
mkg-58282	186	14	factorimmobilized	factorimmobilize	VERB
mkg-58282	186	15	surfaces	surface	NOUN
mkg-58282	186	16	for	for	ADP
mkg-58282	186	17	the	the	DET
mkg-58282	186	18	selective	selective	ADJ
mkg-58282	186	19	expansion	expansion	NOUN
mkg-58282	186	20	of	of	ADP
mkg-58282	186	21	neural	neural	ADJ
mkg-58282	186	22	progenitor	progenitor	NOUN
mkg-58282	186	23	cells	cell	NOUN
mkg-58282	186	24	derived	derive	VERB
mkg-58282	186	25	from	from	ADP
mkg-58282	186	26	induced	induced	ADJ
mkg-58282	186	27	pluripotent	pluripotent	ADJ
mkg-58282	186	28	stem	stem	NOUN
mkg-58282	186	29	cells	cell	NOUN
mkg-58282	186	30	.	.	PUNCT
mkg-58282	187	1	biotechnol	biotechnol	PROPN
mkg-58282	187	2	bioeng	bioeng	PROPN
mkg-58282	187	3	.	.	PUNCT
mkg-58282	187	4	2020	2020	NUM
mkg-58282	187	5	;	;	PUNCT
mkg-58282	187	6	117(9	117(9	NUM
mkg-58282	187	7	):	):	PUNCT
mkg-58282	187	8	2741–8	2741–8	NUM
mkg-58282	187	9	.	.	NOUN
mkg-58282	187	10	21	21	NUM
mkg-58282	187	11	.	.	PUNCT
mkg-58282	188	1	mavridis	mavridis	PROPN
mkg-58282	188	2	l	l	PROPN
mkg-58282	188	3	,	,	PUNCT
mkg-58282	188	4	janes	janes	PROPN
mkg-58282	188	5	rw	rw	PROPN
mkg-58282	188	6	.	.	PUNCT
mkg-58282	189	1	pdb2cd	pdb2cd	PROPN
mkg-58282	189	2	:	:	PUNCT
mkg-58282	189	3	a	a	DET
mkg-58282	189	4	web	web	NOUN
mkg-58282	189	5	-	-	PUNCT
mkg-58282	189	6	based	base	VERB
mkg-58282	189	7	application	application	NOUN
mkg-58282	189	8	for	for	ADP
mkg-58282	189	9	the	the	DET
mkg-58282	189	10	generation	generation	NOUN
mkg-58282	189	11	of	of	ADP
mkg-58282	189	12	circular	circular	ADJ
mkg-58282	189	13	dichroism	dichroism	NOUN
mkg-58282	189	14	spectra	spectra	NOUN
mkg-58282	189	15	from	from	ADP
mkg-58282	189	16	protein	protein	NOUN
mkg-58282	189	17	atomic	atomic	ADJ
mkg-58282	189	18	coordinates	coordinate	NOUN
mkg-58282	189	19	.	.	PUNCT
mkg-58282	190	1	bioinformatics	bioinformatics	NOUN
mkg-58282	190	2	.	.	PUNCT
mkg-58282	191	1	2017	2017	NUM
mkg-58282	191	2	;	;	PUNCT
mkg-58282	192	1	33(1	33(1	NUM
mkg-58282	192	2	):	):	PUNCT
mkg-58282	192	3	56–63	56–63	NUM
mkg-58282	192	4	.	.	PUNCT
mkg-58282	193	1	copyright	copyright	NOUN
mkg-58282	193	2	©	©	PROPN
mkg-58282	193	3	2025	2025	NUM
mkg-58282	193	4	dental	dental	ADJ
mkg-58282	193	5	journal	journal	NOUN
mkg-58282	193	6	(	(	PUNCT
mkg-58282	193	7	majalah	majalah	PROPN
mkg-58282	193	8	kedokteran	kedokteran	PROPN
mkg-58282	193	9	gigi	gigi	PROPN
mkg-58282	193	10	)	)	PUNCT
mkg-58282	194	1	p	p	X
mkg-58282	194	2	-	-	PUNCT
mkg-58282	194	3	issn	issn	NOUN
mkg-58282	194	4	:	:	PUNCT
mkg-58282	194	5	1978	1978	NUM
mkg-58282	194	6	-	-	SYM
mkg-58282	194	7	3728	3728	NUM
mkg-58282	194	8	;	;	PUNCT
mkg-58282	194	9	e	e	X
mkg-58282	194	10	-	-	PUNCT
mkg-58282	194	11	issn	issn	ADJ
mkg-58282	194	12	:	:	PUNCT
mkg-58282	194	13	2442	2442	NUM
mkg-58282	194	14	-	-	SYM
mkg-58282	194	15	9740	9740	NUM
mkg-58282	194	16	.	.	PUNCT
mkg-58282	195	1	accredited	accredit	VERB
mkg-58282	195	2	no	no	INTJ
mkg-58282	195	3	.	.	PROPN
mkg-58282	196	1	158	158	NUM
mkg-58282	196	2	/	/	SYM
mkg-58282	196	3	e	e	NOUN
mkg-58282	196	4	/	/	SYM
mkg-58282	196	5	kpt/2021	kpt/2021	NOUN
mkg-58282	196	6	.	.	PUNCT
mkg-58282	197	1	open	open	ADJ
mkg-58282	197	2	access	access	NOUN
mkg-58282	197	3	under	under	ADP
mkg-58282	197	4	cc	cc	NOUN
mkg-58282	197	5	-	-	PUNCT
mkg-58282	197	6	by	by	ADP
mkg-58282	197	7	-	-	PUNCT
mkg-58282	197	8	sa	sa	NOUN
mkg-58282	197	9	license	license	NOUN
mkg-58282	197	10	.	.	PUNCT
mkg-58282	198	1	available	available	ADJ
mkg-58282	198	2	at	at	ADP
mkg-58282	198	3	https://e-journal.unair.ac.id/mkg/index	https://e-journal.unair.ac.id/mkg/index	NOUN
mkg-58282	198	4	doi	doi	NOUN
mkg-58282	198	5	:	:	PUNCT
mkg-58282	198	6	10.20473	10.20473	NUM
mkg-58282	198	7	/	/	SYM
mkg-58282	198	8	j.djmkg.v58.i3.p219–223	j.djmkg.v58.i3.p219–223	NOUN
mkg-58282	198	9	https://e-journal.unair.ac.id/mkg/index	https://e-journal.unair.ac.id/mkg/index	NOUN
mkg-58282	198	10	https://doi.org/10.20473/j.djmkg.v58.i3.p219-223	https://doi.org/10.20473/j.djmkg.v58.i3.p219-223	NOUN