id	sid	tid	token	lemma	pos
mkg-997	1	1	123	123	NUM
mkg-997	1	2	vol	vol	NOUN
mkg-997	1	3	.	.	PUNCT
mkg-997	2	1	41	41	NUM
mkg-997	2	2	.	.	PUNCT
mkg-997	3	1	no	no	INTJ
mkg-997	3	2	.	.	NOUN
mkg-997	4	1	3	3	NUM
mkg-997	4	2	july	july	PROPN
mkg-997	4	3	–	–	PUNCT
mkg-997	4	4	september	september	PROPN
mkg-997	4	5	2008	2008	NUM
mkg-997	4	6	isolation	isolation	NOUN
mkg-997	4	7	and	and	CCONJ
mkg-997	4	8	identification	identification	NOUN
mkg-997	4	9	of	of	ADP
mkg-997	4	10	java	java	PROPN
mkg-997	4	11	race	race	PROPN
mkg-997	4	12	amniotic	amniotic	ADJ
mkg-997	4	13	membrane	membrane	NOUN
mkg-997	4	14	secretory	secretory	ADJ
mkg-997	4	15	leukocyte	leukocyte	NOUN
mkg-997	4	16	protease	protease	NOUN
mkg-997	4	17	inhibitor	inhibitor	NOUN
mkg-997	4	18	gene	gene	NOUN
mkg-997	4	19	elly	elly	ADV
mkg-997	4	20	munadziroh	munadziroh	PROPN
mkg-997	4	21	department	department	NOUN
mkg-997	4	22	of	of	ADP
mkg-997	4	23	dental	dental	ADJ
mkg-997	4	24	material	material	NOUN
mkg-997	4	25	and	and	CCONJ
mkg-997	4	26	technology	technology	NOUN
mkg-997	4	27	faculty	faculty	NOUN
mkg-997	4	28	of	of	ADP
mkg-997	4	29	dentistry	dentistry	NOUN
mkg-997	4	30	airlangga	airlangga	PROPN
mkg-997	4	31	university	university	PROPN
mkg-997	4	32	surabaya	surabaya	PROPN
mkg-997	4	33	indonesia	indonesia	PROPN
mkg-997	4	34	abstract	abstract	ADJ
mkg-997	4	35	background	background	NOUN
mkg-997	4	36	:	:	PUNCT
mkg-997	4	37	secretory	secretory	ADJ
mkg-997	4	38	leukocyte	leukocyte	NOUN
mkg-997	4	39	protease	protease	NOUN
mkg-997	4	40	inhibitor	inhibitor	NOUN
mkg-997	4	41	(	(	PUNCT
mkg-997	4	42	slpi	slpi	NOUN
mkg-997	4	43	)	)	PUNCT
mkg-997	4	44	has	have	AUX
mkg-997	4	45	been	be	AUX
mkg-997	4	46	found	find	VERB
mkg-997	4	47	to	to	PART
mkg-997	4	48	facilitate	facilitate	VERB
mkg-997	4	49	epithelialization	epithelialization	NOUN
mkg-997	4	50	,	,	PUNCT
mkg-997	4	51	maintain	maintain	VERB
mkg-997	4	52	a	a	DET
mkg-997	4	53	normal	normal	ADJ
mkg-997	4	54	secretory	secretory	ADJ
mkg-997	4	55	leukocyte	leukocyte	NOUN
mkg-997	4	56	protease	protease	NOUN
mkg-997	4	57	inhibitor	inhibitor	NOUN
mkg-997	4	58	(	(	PUNCT
mkg-997	4	59	slpi	slpi	NOUN
mkg-997	4	60	)	)	PUNCT
mkg-997	4	61	has	have	AUX
mkg-997	4	62	been	be	AUX
mkg-997	4	63	found	find	VERB
mkg-997	4	64	to	to	PART
mkg-997	4	65	facilitate	facilitate	VERB
mkg-997	4	66	epithelialization	epithelialization	NOUN
mkg-997	4	67	,	,	PUNCT
mkg-997	4	68	maintain	maintain	VERB
mkg-997	4	69	a	a	DET
mkg-997	4	70	normal	normal	ADJ
mkg-997	4	71	epithelial	epithelial	ADJ
mkg-997	4	72	phenotype	phenotype	NOUN
mkg-997	4	73	,	,	PUNCT
mkg-997	4	74	reduce	reduce	VERB
mkg-997	4	75	inflammation	inflammation	NOUN
mkg-997	4	76	,	,	PUNCT
mkg-997	4	77	secrete	secrete	ADJ
mkg-997	4	78	growth	growth	NOUN
mkg-997	4	79	factors	factor	NOUN
mkg-997	4	80	such	such	ADJ
mkg-997	4	81	as	as	ADP
mkg-997	4	82	il-4	il-4	NOUN
mkg-997	4	83	,	,	PUNCT
mkg-997	4	84	il-6	il-6	NUM
mkg-997	4	85	,	,	PUNCT
mkg-997	4	86	il-10	il-10	PRON
mkg-997	4	87	,	,	PUNCT
mkg-997	4	88	egf	egf	PROPN
mkg-997	4	89	,	,	PUNCT
mkg-997	4	90	fgf	fgf	PROPN
mkg-997	4	91	,	,	PUNCT
mkg-997	4	92	tgf	tgf	PROPN
mkg-997	4	93	,	,	PUNCT
mkg-997	4	94	hgfand	hgfand	NOUN
mkg-997	4	95	2	2	NUM
mkg-997	4	96	-	-	PUNCT
mkg-997	4	97	microbulin	microbulin	NOUN
mkg-997	4	98	.	.	PUNCT
mkg-997	5	1	slpi	slpi	NOUN
mkg-997	5	2	is	be	AUX
mkg-997	5	3	serine	serine	ADJ
mkg-997	5	4	protease	protease	NOUN
mkg-997	5	5	inhibitor	inhibitor	NOUN
mkg-997	5	6	,	,	PUNCT
mkg-997	5	7	which	which	PRON
mkg-997	5	8	found	find	VERB
mkg-997	5	9	in	in	ADP
mkg-997	5	10	secretions	secretion	NOUN
mkg-997	5	11	such	such	ADJ
mkg-997	5	12	as	as	ADP
mkg-997	5	13	whole	whole	ADJ
mkg-997	5	14	saliva	saliva	NOUN
mkg-997	5	15	,	,	PUNCT
mkg-997	5	16	seminal	seminal	ADJ
mkg-997	5	17	fluid	fluid	NOUN
mkg-997	5	18	,	,	PUNCT
mkg-997	5	19	cervical	cervical	ADJ
mkg-997	5	20	mucus	mucus	NOUN
mkg-997	5	21	,	,	PUNCT
mkg-997	5	22	synovial	synovial	ADJ
mkg-997	5	23	fluid	fluid	NOUN
mkg-997	5	24	,	,	PUNCT
mkg-997	5	25	breast	breast	NOUN
mkg-997	5	26	milk	milk	NOUN
mkg-997	5	27	,	,	PUNCT
mkg-997	5	28	tears	tear	NOUN
mkg-997	5	29	,	,	PUNCT
mkg-997	5	30	amniotic	amniotic	ADJ
mkg-997	5	31	fluid	fluid	NOUN
mkg-997	5	32	and	and	CCONJ
mkg-997	5	33	amniotic	amniotic	ADJ
mkg-997	5	34	membrane	membrane	NOUN
mkg-997	5	35	.	.	PUNCT
mkg-997	6	1	impaired	impair	VERB
mkg-997	6	2	healing	healing	NOUN
mkg-997	6	3	states	state	NOUN
mkg-997	6	4	are	be	AUX
mkg-997	6	5	characterized	characterize	VERB
mkg-997	6	6	by	by	ADP
mkg-997	6	7	excessive	excessive	ADJ
mkg-997	6	8	proteolysis	proteolysis	NOUN
mkg-997	6	9	and	and	CCONJ
mkg-997	6	10	oftenbacterial	oftenbacterial	ADJ
mkg-997	6	11	infection	infection	NOUN
mkg-997	6	12	,	,	PUNCT
mkg-997	6	13	leading	lead	VERB
mkg-997	6	14	to	to	ADP
mkg-997	6	15	the	the	DET
mkg-997	6	16	hypothesis	hypothesis	NOUN
mkg-997	6	17	that	that	PRON
mkg-997	6	18	slpi	slpi	NOUN
mkg-997	6	19	may	may	AUX
mkg-997	6	20	have	have	VERB
mkg-997	6	21	a	a	DET
mkg-997	6	22	role	role	NOUN
mkg-997	6	23	in	in	ADP
mkg-997	6	24	the	the	DET
mkg-997	6	25	healing	healing	NOUN
mkg-997	6	26	process	process	NOUN
mkg-997	6	27	in	in	ADP
mkg-997	6	28	oral	oral	ADJ
mkg-997	6	29	inflammation	inflammation	NOUN
mkg-997	6	30	and	and	CCONJ
mkg-997	6	31	contributes	contribute	VERB
mkg-997	6	32	to	to	ADP
mkg-997	6	33	tissue	tissue	NOUN
mkg-997	6	34	repair	repair	NOUN
mkg-997	6	35	in	in	ADP
mkg-997	6	36	oral	oral	ADJ
mkg-997	6	37	mucosa	mucosa	NOUN
mkg-997	6	38	.	.	PUNCT
mkg-997	7	1	the	the	DET
mkg-997	7	2	oral	oral	ADJ
mkg-997	7	3	wound	wound	NOUN
mkg-997	7	4	healing	healing	NOUN
mkg-997	7	5	response	response	NOUN
mkg-997	7	6	is	be	AUX
mkg-997	7	7	impaired	impair	VERB
mkg-997	7	8	in	in	ADP
mkg-997	7	9	the	the	DET
mkg-997	7	10	slpi	slpi	NOUN
mkg-997	7	11	sufficient	sufficient	ADJ
mkg-997	7	12	mice	mouse	NOUN
mkg-997	7	13	since	since	SCONJ
mkg-997	7	14	matrix	matrix	NOUN
mkg-997	7	15	synthesis	synthesis	NOUN
mkg-997	7	16	and	and	CCONJ
mkg-997	7	17	collagen	collagen	NOUN
mkg-997	7	18	deposition	deposition	NOUN
mkg-997	7	19	delayed	delay	VERB
mkg-997	7	20	.	.	PUNCT
mkg-997	8	1	the	the	DET
mkg-997	8	2	ob	ob	PROPN
mkg-997	8	3	�	�	PROPN
mkg-997	8	4	ective	ective	NOUN
mkg-997	8	5	of	of	ADP
mkg-997	8	6	this	this	DET
mkg-997	8	7	research	research	NOUN
mkg-997	8	8	is	be	AUX
mkg-997	8	9	to	to	PART
mkg-997	8	10	isolate	isolate	VERB
mkg-997	8	11	and	and	CCONJ
mkg-997	8	12	identify	identify	VERB
mkg-997	8	13	the	the	DET
mkg-997	8	14	amniotic	amniotic	ADJ
mkg-997	8	15	membrane	membrane	NOUN
mkg-997	8	16	of	of	ADP
mkg-997	8	17	java	java	PROPN
mkg-997	8	18	race	race	NOUN
mkg-997	8	19	slpi	slpi	NOUN
mkg-997	8	20	gene	gene	NOUN
mkg-997	8	21	.	.	PUNCT
mkg-997	9	1	methods	method	NOUN
mkg-997	9	2	:	:	PUNCT
mkg-997	9	3	slpi	slpi	PROPN
mkg-997	9	4	rna	rna	PROPN
mkg-997	9	5	was	be	AUX
mkg-997	9	6	isolated	isolate	VERB
mkg-997	9	7	from	from	ADP
mkg-997	9	8	java	java	PROPN
mkg-997	9	9	race	race	PROPN
mkg-997	9	10	amniotic	amniotic	ADJ
mkg-997	9	11	membrane	membrane	NOUN
mkg-997	9	12	and	and	CCONJ
mkg-997	9	13	the	the	DET
mkg-997	9	14	cdna	cdna	PROPN
mkg-997	9	15	was	be	AUX
mkg-997	9	16	amplified	amplify	VERB
mkg-997	9	17	by	by	ADP
mkg-997	9	18	polymerase	polymerase	NOUN
mkg-997	9	19	chain	chain	NOUN
mkg-997	9	20	reaction	reaction	NOUN
mkg-997	9	21	slpi	slpi	PROPN
mkg-997	9	22	rna	rna	PROPN
mkg-997	9	23	was	be	AUX
mkg-997	9	24	isolated	isolate	VERB
mkg-997	9	25	from	from	ADP
mkg-997	9	26	java	java	PROPN
mkg-997	9	27	race	race	PROPN
mkg-997	9	28	amniotic	amniotic	ADJ
mkg-997	9	29	membrane	membrane	NOUN
mkg-997	9	30	and	and	CCONJ
mkg-997	9	31	the	the	DET
mkg-997	9	32	cdna	cdna	PROPN
mkg-997	9	33	was	be	AUX
mkg-997	9	34	amplified	amplify	VERB
mkg-997	9	35	by	by	ADP
mkg-997	9	36	polymerase	polymerase	NOUN
mkg-997	9	37	chain	chain	NOUN
mkg-997	9	38	reaction	reaction	NOUN
mkg-997	9	39	(	(	PUNCT
mkg-997	9	40	pcr	pcr	PROPN
mkg-997	9	41	)	)	PUNCT
mkg-997	9	42	.	.	PUNCT
mkg-997	10	1	result	result	VERB
mkg-997	10	2	:	:	PUNCT
mkg-997	10	3	through	through	ADP
mkg-997	10	4	sequence	sequence	NOUN
mkg-997	10	5	analyses	analysis	NOUN
mkg-997	10	6	,	,	PUNCT
mkg-997	10	7	slpi	slpi	NOUN
mkg-997	10	8	cdna	cdna	PROPN
mkg-997	10	9	was	be	AUX
mkg-997	10	10	530	530	NUM
mkg-997	10	11	nucleotide	nucleotide	NOUN
mkg-997	10	12	in	in	ADP
mkg-997	10	13	length	length	NOUN
mkg-997	10	14	with	with	ADP
mkg-997	10	15	a	a	DET
mkg-997	10	16	predicted	predict	VERB
mkg-997	10	17	molecular	molecular	ADJ
mkg-997	10	18	mass	mass	NOUN
mkg-997	10	19	about	about	ADV
mkg-997	10	20	12	12	NUM
mkg-997	10	21	kda	kda	NOUN
mkg-997	10	22	.	.	PUNCT
mkg-997	11	1	through	through	ADP
mkg-997	11	2	sequence	sequence	NOUN
mkg-997	11	3	analyses	analysis	NOUN
mkg-997	11	4	,	,	PUNCT
mkg-997	11	5	slpi	slpi	NOUN
mkg-997	11	6	cdna	cdna	PROPN
mkg-997	11	7	was	be	AUX
mkg-997	11	8	530	530	NUM
mkg-997	11	9	nucleotide	nucleotide	NOUN
mkg-997	11	10	in	in	ADP
mkg-997	11	11	length	length	NOUN
mkg-997	11	12	with	with	ADP
mkg-997	11	13	a	a	DET
mkg-997	11	14	predicted	predict	VERB
mkg-997	11	15	molecular	molecular	ADJ
mkg-997	11	16	mass	mass	NOUN
mkg-997	11	17	about	about	ADV
mkg-997	11	18	12	12	NUM
mkg-997	11	19	kda	kda	NOUN
mkg-997	11	20	.	.	PUNCT
mkg-997	12	1	the	the	DET
mkg-997	12	2	nucleotide	nucleotide	ADJ
mkg-997	12	3	sequence	sequence	NOUN
mkg-997	12	4	showed	show	VERB
mkg-997	12	5	that	that	SCONJ
mkg-997	12	6	human	human	ADJ
mkg-997	12	7	slpi	slpi	NOUN
mkg-997	12	8	from	from	ADP
mkg-997	12	9	sample	sample	NOUN
mkg-997	12	10	was	be	AUX
mkg-997	12	11	98	98	NUM
mkg-997	12	12	%	%	NOUN
mkg-997	12	13	identical	identical	ADJ
mkg-997	12	14	with	with	ADP
mkg-997	12	15	human	human	ADJ
mkg-997	12	16	slpi	slpi	NOUN
mkg-997	12	17	from	from	ADP
mkg-997	12	18	gene	gene	NOUN
mkg-997	12	19	bank	bank	NOUN
mkg-997	12	20	.	.	PUNCT
mkg-997	13	1	pcr	pcr	ADJ
mkg-997	13	2	analysis	analysis	NOUN
mkg-997	13	3	revealed	reveal	VERB
mkg-997	13	4	that	that	SCONJ
mkg-997	13	5	the	the	DET
mkg-997	13	6	mrna	mrna	NOUN
mkg-997	13	7	of	of	ADP
mkg-997	13	8	slpi	slpi	NOUN
mkg-997	13	9	was	be	AUX
mkg-997	13	10	highly	highly	ADV
mkg-997	13	11	expressed	express	VERB
mkg-997	13	12	in	in	ADP
mkg-997	13	13	the	the	DET
mkg-997	13	14	amniotic	amniotic	ADJ
mkg-997	13	15	membrane	membrane	NOUN
mkg-997	13	16	from	from	ADP
mkg-997	13	17	java	java	PROPN
mkg-997	13	18	race	race	NOUN
mkg-997	13	19	sample	sample	NOUN
mkg-997	13	20	.	.	PUNCT
mkg-997	14	1	conclusion	conclusion	NOUN
mkg-997	14	2	:	:	PUNCT
mkg-997	14	3	it	it	PRON
mkg-997	14	4	is	be	AUX
mkg-997	14	5	demonstrated	demonstrate	VERB
mkg-997	14	6	it	it	PRON
mkg-997	14	7	is	be	AUX
mkg-997	14	8	demonstrated	demonstrate	VERB
mkg-997	14	9	that	that	SCONJ
mkg-997	14	10	human	human	ADJ
mkg-997	14	11	slpi	slpi	NOUN
mkg-997	14	12	are	be	AUX
mkg-997	14	13	highly	highly	ADV
mkg-997	14	14	conserved	conserved	ADJ
mkg-997	14	15	in	in	ADP
mkg-997	14	16	sequence	sequence	NOUN
mkg-997	14	17	content	content	NOUN
mkg-997	14	18	as	as	SCONJ
mkg-997	14	19	compared	compare	VERB
mkg-997	14	20	to	to	ADP
mkg-997	14	21	the	the	DET
mkg-997	14	22	human	human	ADJ
mkg-997	14	23	slpi	slpi	NOUN
mkg-997	14	24	from	from	ADP
mkg-997	14	25	gene	gene	NOUN
mkg-997	14	26	.	.	PUNCT
mkg-997	15	1	key	key	ADJ
mkg-997	15	2	words	word	NOUN
mkg-997	15	3	:	:	PUNCT
mkg-997	15	4	secretory	secretory	ADJ
mkg-997	15	5	leukocyte	leukocyte	NOUN
mkg-997	15	6	protease	protease	NOUN
mkg-997	15	7	inhibitor	inhibitor	NOUN
mkg-997	15	8	,	,	PUNCT
mkg-997	15	9	amniotic	amniotic	ADJ
mkg-997	15	10	membrane	membrane	NOUN
mkg-997	15	11	,	,	PUNCT
mkg-997	15	12	cloning	cloning	NOUN
mkg-997	15	13	,	,	PUNCT
mkg-997	15	14	recombinant	recombinant	ADJ
mkg-997	15	15	,	,	PUNCT
mkg-997	15	16	wound	wound	ADJ
mkg-997	15	17	healing	healing	NOUN
mkg-997	15	18	correspondence	correspondence	NOUN
mkg-997	15	19	:	:	PUNCT
mkg-997	15	20	elly	elly	ADV
mkg-997	15	21	munadziroh	munadziroh	NOUN
mkg-997	15	22	,	,	PUNCT
mkg-997	15	23	c	c	X
mkg-997	15	24	/	/	SYM
mkg-997	15	25	o	o	NOUN
mkg-997	15	26	:	:	PUNCT
mkg-997	15	27	departemen	departeman	NOUN
mkg-997	15	28	ilmu	ilmu	PROPN
mkg-997	15	29	material	material	PROPN
mkg-997	15	30	kedokteran	kedokteran	PROPN
mkg-997	15	31	gigi	gigi	PROPN
mkg-997	15	32	.	.	PUNCT
mkg-997	16	1	jln	jln	PROPN
mkg-997	16	2	.	.	PROPN
mkg-997	16	3	mayjend	mayjend	PROPN
mkg-997	16	4	.	.	PUNCT
mkg-997	17	1	prof	prof	PROPN
mkg-997	17	2	.	.	PUNCT
mkg-997	18	1	moestopo	moestopo	PROPN
mkg-997	19	1	no	no	NOUN
mkg-997	19	2	.	.	PUNCT
mkg-997	20	1	47	47	NUM
mkg-997	20	2	surabaya	surabaya	PROPN
mkg-997	20	3	60132	60132	NUM
mkg-997	20	4	,	,	PUNCT
mkg-997	20	5	indonesia	indonesia	PROPN
mkg-997	20	6	.	.	PUNCT
mkg-997	20	7	email	email	NOUN
mkg-997	20	8	:	:	PUNCT
mkg-997	21	1	emunadziroh@yahoo.com	emunadziroh@yahoo.com	X
mkg-997	21	2	introduction	introduction	NOUN
mkg-997	21	3	gingival	gingival	NOUN
mkg-997	21	4	recession	recession	NOUN
mkg-997	21	5	occurs	occur	VERB
mkg-997	21	6	at	at	ADP
mkg-997	21	7	all	all	DET
mkg-997	21	8	ages	age	NOUN
mkg-997	21	9	varied	varied	ADJ
mkg-997	21	10	from	from	ADP
mkg-997	21	11	65	65	NUM
mkg-997	21	12	years	year	NOUN
mkg-997	21	13	old	old	ADJ
mkg-997	21	14	or	or	CCONJ
mkg-997	21	15	higher	high	ADJ
mkg-997	21	16	(	(	PUNCT
mkg-997	21	17	88	88	NUM
mkg-997	21	18	%	%	NOUN
mkg-997	21	19	)	)	PUNCT
mkg-997	21	20	and	and	CCONJ
mkg-997	21	21	18–64	18–64	NUM
mkg-997	21	22	years	year	NOUN
mkg-997	21	23	old	old	ADJ
mkg-997	21	24	(	(	PUNCT
mkg-997	21	25	50%).1	50%).1	NUM
mkg-997	21	26	surgical	surgical	ADJ
mkg-997	21	27	treatment	treatment	NOUN
mkg-997	21	28	of	of	ADP
mkg-997	21	29	gingival	gingival	NOUN
mkg-997	21	30	recession	recession	NOUN
mkg-997	21	31	cause	cause	VERB
mkg-997	21	32	fairly	fairly	ADV
mkg-997	21	33	large	large	ADJ
mkg-997	21	34	muco	muco	ADJ
mkg-997	21	35	gingival	gingival	NOUN
mkg-997	21	36	injury	injury	NOUN
mkg-997	21	37	due	due	ADP
mkg-997	21	38	to	to	ADP
mkg-997	21	39	gingival	gingival	NOUN
mkg-997	21	40	retraction	retraction	NOUN
mkg-997	21	41	toward	toward	ADP
mkg-997	21	42	coronal	coronal	NOUN
mkg-997	21	43	.	.	PUNCT
mkg-997	22	1	tension	tension	NOUN
mkg-997	22	2	will	will	AUX
mkg-997	22	3	lead	lead	VERB
mkg-997	22	4	to	to	ADP
mkg-997	22	5	excessive	excessive	ADJ
mkg-997	22	6	thin	thin	ADJ
mkg-997	22	7	gingival	gingival	NOUN
mkg-997	22	8	and	and	CCONJ
mkg-997	22	9	laceration.2	laceration.2	PROPN
mkg-997	22	10	in	in	ADP
mkg-997	22	11	the	the	DET
mkg-997	22	12	oral	oral	ADJ
mkg-997	22	13	cavity	cavity	NOUN
mkg-997	22	14	,	,	PUNCT
mkg-997	22	15	bacterial	bacterial	ADJ
mkg-997	22	16	free	free	ADJ
mkg-997	22	17	environment	environment	NOUN
mkg-997	22	18	is	be	AUX
mkg-997	22	19	difficult	difficult	ADJ
mkg-997	22	20	to	to	PART
mkg-997	22	21	achieve	achieve	VERB
mkg-997	22	22	.	.	PUNCT
mkg-997	23	1	further	further	ADJ
mkg-997	23	2	complication	complication	NOUN
mkg-997	23	3	would	would	AUX
mkg-997	23	4	occur	occur	VERB
mkg-997	23	5	if	if	SCONJ
mkg-997	23	6	immediate	immediate	ADJ
mkg-997	23	7	treatment	treatment	NOUN
mkg-997	23	8	is	be	AUX
mkg-997	23	9	not	not	PART
mkg-997	23	10	carried	carry	VERB
mkg-997	23	11	out	out	ADP
mkg-997	23	12	since	since	SCONJ
mkg-997	23	13	it	it	PRON
mkg-997	23	14	would	would	AUX
mkg-997	23	15	be	be	AUX
mkg-997	23	16	easily	easily	ADV
mkg-997	23	17	to	to	PART
mkg-997	23	18	be	be	AUX
mkg-997	23	19	contaminated	contaminate	VERB
mkg-997	23	20	by	by	ADP
mkg-997	23	21	bacteria	bacteria	NOUN
mkg-997	23	22	,	,	PUNCT
mkg-997	23	23	leading	lead	VERB
mkg-997	23	24	to	to	ADP
mkg-997	23	25	cellulites	cellulite	NOUN
mkg-997	23	26	,	,	PUNCT
mkg-997	23	27	abscess	abscess	NOUN
mkg-997	23	28	or	or	CCONJ
mkg-997	23	29	osteomyelitis	osteomyelitis	NOUN
mkg-997	23	30	.	.	PUNCT
mkg-997	24	1	various	various	ADJ
mkg-997	24	2	method	method	NOUN
mkg-997	24	3	have	have	AUX
mkg-997	24	4	been	be	AUX
mkg-997	24	5	developed	develop	VERB
mkg-997	24	6	to	to	PART
mkg-997	24	7	achieve	achieve	VERB
mkg-997	24	8	injury	injury	NOUN
mkg-997	24	9	recovery.3,4	recovery.3,4	ADJ
mkg-997	24	10	the	the	DET
mkg-997	24	11	present	present	ADJ
mkg-997	24	12	concept	concept	NOUN
mkg-997	24	13	of	of	ADP
mkg-997	24	14	treatment	treatment	NOUN
mkg-997	24	15	and	and	CCONJ
mkg-997	24	16	recovery	recovery	NOUN
mkg-997	24	17	has	have	AUX
mkg-997	24	18	been	be	AUX
mkg-997	24	19	developed	develop	VERB
mkg-997	24	20	simultaneously	simultaneously	ADV
mkg-997	24	21	with	with	ADP
mkg-997	24	22	dental	dental	ADJ
mkg-997	24	23	biomaterial	biomaterial	ADJ
mkg-997	24	24	progress	progress	NOUN
mkg-997	24	25	.	.	PUNCT
mkg-997	25	1	biological	biological	ADJ
mkg-997	25	2	material	material	NOUN
mkg-997	25	3	is	be	AUX
mkg-997	25	4	currently	currently	ADV
mkg-997	25	5	considered	consider	VERB
mkg-997	25	6	to	to	PART
mkg-997	25	7	be	be	AUX
mkg-997	25	8	applied	apply	VERB
mkg-997	25	9	due	due	ADP
mkg-997	25	10	to	to	ADP
mkg-997	25	11	the	the	DET
mkg-997	25	12	supporting	support	VERB
mkg-997	25	13	component	component	NOUN
mkg-997	25	14	of	of	ADP
mkg-997	25	15	treatment	treatment	NOUN
mkg-997	25	16	and	and	CCONJ
mkg-997	25	17	recovery	recovery	NOUN
mkg-997	25	18	.	.	PUNCT
mkg-997	26	1	one	one	NUM
mkg-997	26	2	of	of	ADP
mkg-997	26	3	the	the	DET
mkg-997	26	4	bio	bio	PROPN
mkg-997	26	5	material	material	PROPN
mkg-997	26	6	expected	expect	VERB
mkg-997	26	7	to	to	PART
mkg-997	26	8	be	be	AUX
mkg-997	26	9	able	able	ADJ
mkg-997	26	10	to	to	PART
mkg-997	26	11	accelerate	accelerate	VERB
mkg-997	26	12	injury	injury	NOUN
mkg-997	26	13	recovery	recovery	NOUN
mkg-997	26	14	is	be	AUX
mkg-997	26	15	amniotic	amniotic	ADJ
mkg-997	26	16	membrane	membrane	NOUN
mkg-997	26	17	that	that	PRON
mkg-997	26	18	effective	effective	ADJ
mkg-997	26	19	in	in	ADP
mkg-997	26	20	burn	burn	NOUN
mkg-997	26	21	injury	injury	NOUN
mkg-997	26	22	treatment	treatment	NOUN
mkg-997	26	23	as	as	ADV
mkg-997	26	24	well	well	ADV
mkg-997	26	25	as	as	ADP
mkg-997	26	26	in	in	ADP
mkg-997	26	27	ophthalmology.5,6,7	ophthalmology.5,6,7	X
mkg-997	26	28	secretory	secretory	ADJ
mkg-997	26	29	leukocyte	leukocyte	NOUN
mkg-997	26	30	protease	protease	NOUN
mkg-997	26	31	inhibitor	inhibitor	NOUN
mkg-997	26	32	(	(	PUNCT
mkg-997	26	33	slpi	slpi	NOUN
mkg-997	26	34	)	)	PUNCT
mkg-997	26	35	has	have	AUX
mkg-997	26	36	been	be	AUX
mkg-997	26	37	found	find	VERB
mkg-997	26	38	to	to	PART
mkg-997	26	39	be	be	AUX
mkg-997	26	40	an	an	DET
mkg-997	26	41	active	active	ADJ
mkg-997	26	42	material	material	NOUN
mkg-997	26	43	of	of	ADP
mkg-997	26	44	amniotic	amniotic	ADJ
mkg-997	26	45	membrane	membrane	NOUN
mkg-997	26	46	expected	expect	VERB
mkg-997	26	47	to	to	PART
mkg-997	26	48	be	be	AUX
mkg-997	26	49	influential	influential	ADJ
mkg-997	26	50	to	to	AUX
mkg-997	26	51	injury	injury	NOUN
mkg-997	26	52	recovery	recovery	NOUN
mkg-997	26	53	.	.	PUNCT
mkg-997	27	1	slpi	slpi	NOUN
mkg-997	27	2	is	be	AUX
mkg-997	27	3	one	one	NUM
mkg-997	27	4	of	of	ADP
mkg-997	27	5	the	the	DET
mkg-997	27	6	inhibitors	inhibitor	NOUN
mkg-997	27	7	of	of	ADP
mkg-997	27	8	serine	serine	NOUN
mkg-997	27	9	in	in	ADP
mkg-997	27	10	addition	addition	NOUN
mkg-997	27	11	to	to	ADP
mkg-997	27	12	a1antitrypsin	a1antitrypsin	PROPN
mkg-997	27	13	(	(	PUNCT
mkg-997	27	14	a1	a1	NOUN
mkg-997	27	15	-	-	PUNCT
mkg-997	27	16	pi	pi	NOUN
mkg-997	27	17	)	)	PUNCT
mkg-997	27	18	and	and	CCONJ
mkg-997	27	19	leafin	leafin	NOUN
mkg-997	27	20	.	.	PUNCT
mkg-997	28	1	the	the	DET
mkg-997	28	2	main	main	ADJ
mkg-997	28	3	target	target	NOUN
mkg-997	28	4	of	of	ADP
mkg-997	28	5	slpi	slpi	NOUN
mkg-997	28	6	is	be	AUX
mkg-997	28	7	to	to	PART
mkg-997	28	8	inhibit	inhibit	VERB
mkg-997	28	9	serine	serine	ADJ
mkg-997	28	10	protease	protease	NOUN
mkg-997	28	11	-	-	PUNCT
mkg-997	28	12	the	the	DET
mkg-997	28	13	human	human	ADJ
mkg-997	28	14	netrophil	netrophil	ADJ
mkg-997	28	15	protease	protease	NOUN
mkg-997	28	16	(	(	PUNCT
mkg-997	28	17	hne	hne	NOUN
mkg-997	28	18	)	)	PUNCT
mkg-997	28	19	including	include	VERB
mkg-997	28	20	chemotripsyn	chemotripsyn	PROPN
mkg-997	28	21	super	super	ADJ
mkg-997	28	22	-	-	ADJ
mkg-997	28	23	family	family	ADJ
mkg-997	28	24	member	member	NOUN
mkg-997	28	25	of	of	ADP
mkg-997	28	26	serine	serine	NOUN
mkg-997	28	27	protease	protease	NOUN
mkg-997	28	28	expressed	express	VERB
mkg-997	28	29	in	in	ADP
mkg-997	28	30	monosite	monosite	NOUN
mkg-997	28	31	and	and	CCONJ
mkg-997	28	32	mast	mast	VERB
mkg-997	28	33	sell	sell	VERB
mkg-997	28	34	especially	especially	ADV
mkg-997	28	35	netrophil	netrophil	ADJ
mkg-997	28	36	.	.	PUNCT
mkg-997	29	1	the	the	DET
mkg-997	29	2	function	function	NOUN
mkg-997	29	3	of	of	ADP
mkg-997	29	4	this	this	DET
mkg-997	29	5	intracellular	intracellular	ADJ
mkg-997	29	6	enzyme	enzyme	NOUN
mkg-997	29	7	is	be	AUX
mkg-997	29	8	to	to	PART
mkg-997	29	9	degrade	degrade	VERB
mkg-997	29	10	foreign	foreign	ADJ
mkg-997	29	11	extra	extra	ADJ
mkg-997	29	12	cellular	cellular	ADJ
mkg-997	29	13	matrix	matrix	NOUN
mkg-997	29	14	(	(	PUNCT
mkg-997	29	15	ecm	ecm	PROPN
mkg-997	29	16	)	)	PUNCT
mkg-997	29	17	,	,	PUNCT
mkg-997	29	18	remodeling	remodel	VERB
mkg-997	29	19	damaged	damage	VERB
mkg-997	29	20	tissue	tissue	NOUN
mkg-997	29	21	and	and	CCONJ
mkg-997	29	22	facilitating	facilitate	VERB
mkg-997	29	23	netrophil	netrophil	ADJ
mkg-997	29	24	migration	migration	NOUN
mkg-997	29	25	into	into	ADP
mkg-997	29	26	other	other	ADJ
mkg-997	29	27	tissue.8	tissue.8	PROPN
mkg-997	29	28	research	research	NOUN
mkg-997	29	29	report	report	NOUN
mkg-997	29	30	124	124	NUM
mkg-997	29	31	dent	dent	NOUN
mkg-997	29	32	.	.	PUNCT
mkg-997	30	1	j.	j.	PROPN
mkg-997	30	2	(	(	PUNCT
mkg-997	30	3	maj	maj	PROPN
mkg-997	30	4	.	.	PROPN
mkg-997	30	5	ked	ked	PROPN
mkg-997	30	6	.	.	PUNCT
mkg-997	31	1	gigi	gigi	PROPN
mkg-997	31	2	)	)	PUNCT
mkg-997	31	3	,	,	PUNCT
mkg-997	31	4	vol	vol	NOUN
mkg-997	31	5	.	.	PROPN
mkg-997	32	1	41	41	NUM
mkg-997	32	2	.	.	PUNCT
mkg-997	33	1	no	no	INTJ
mkg-997	33	2	.	.	NOUN
mkg-997	34	1	3	3	NUM
mkg-997	34	2	july	july	PROPN
mkg-997	34	3	–	–	PUNCT
mkg-997	34	4	september	september	PROPN
mkg-997	34	5	2008	2008	NUM
mkg-997	34	6	:	:	PUNCT
mkg-997	34	7	123	123	NUM
mkg-997	34	8	-	-	SYM
mkg-997	34	9	127	127	NUM
mkg-997	34	10	additional	additional	ADJ
mkg-997	34	11	function	function	NOUN
mkg-997	34	12	of	of	ADP
mkg-997	34	13	slpi	slpi	NOUN
mkg-997	34	14	is	be	AUX
mkg-997	34	15	to	to	PART
mkg-997	34	16	inhibit	inhibit	VERB
mkg-997	34	17	leukocyte	leukocyte	ADJ
mkg-997	34	18	proteinase	proteinase	NOUN
mkg-997	34	19	,	,	PUNCT
mkg-997	34	20	to	to	PART
mkg-997	34	21	maintain	maintain	VERB
mkg-997	34	22	immunity	immunity	NOUN
mkg-997	34	23	against	against	ADP
mkg-997	34	24	infection	infection	NOUN
mkg-997	34	25	,	,	PUNCT
mkg-997	34	26	regulating	regulate	VERB
mkg-997	34	27	leukocyte	leukocyte	NOUN
mkg-997	34	28	function	function	NOUN
mkg-997	34	29	,	,	PUNCT
mkg-997	34	30	producing	produce	VERB
mkg-997	34	31	matrix	matrix	NOUN
mkg-997	34	32	and	and	CCONJ
mkg-997	34	33	improving	improve	VERB
mkg-997	34	34	tissue	tissue	NOUN
mkg-997	34	35	.	.	PUNCT
mkg-997	35	1	slpi	slpi	NOUN
mkg-997	35	2	also	also	ADV
mkg-997	35	3	facilitates	facilitate	VERB
mkg-997	35	4	several	several	ADJ
mkg-997	35	5	functions	function	NOUN
mkg-997	35	6	:	:	PUNCT
mkg-997	35	7	inhibiting	inhibit	VERB
mkg-997	35	8	protease	protease	NOUN
mkg-997	35	9	,	,	PUNCT
mkg-997	35	10	controlling	control	VERB
mkg-997	35	11	leukocyte	leukocyte	NOUN
mkg-997	35	12	activity	activity	NOUN
mkg-997	35	13	,	,	PUNCT
mkg-997	35	14	reducing	reduce	VERB
mkg-997	35	15	tgf	tgf	NOUN
mkg-997	35	16	-	-	PUNCT
mkg-997	35	17	beta	beta	NOUN
mkg-997	35	18	,	,	PUNCT
mkg-997	35	19	anti	anti	X
mkg-997	35	20	inflammatory	inflammatory	ADJ
mkg-997	35	21	,	,	PUNCT
mkg-997	35	22	anti	anti	ADJ
mkg-997	35	23	bacteria	bacteria	NOUN
mkg-997	35	24	,	,	PUNCT
mkg-997	35	25	anti	anti	ADJ
mkg-997	35	26	retroviral	retroviral	NOUN
mkg-997	35	27	(	(	PUNCT
mkg-997	35	28	human	human	ADJ
mkg-997	35	29	immunodeficiency	immunodeficiency	NOUN
mkg-997	35	30	virus	virus	NOUN
mkg-997	35	31	)	)	PUNCT
mkg-997	35	32	.	.	PUNCT
mkg-997	36	1	another	another	DET
mkg-997	36	2	function	function	NOUN
mkg-997	36	3	of	of	ADP
mkg-997	36	4	slpi	slpi	NOUN
mkg-997	36	5	are	be	AUX
mkg-997	36	6	to	to	PART
mkg-997	36	7	control	control	VERB
mkg-997	36	8	synthesis	synthesis	NOUN
mkg-997	36	9	of	of	ADP
mkg-997	36	10	intracellular	intracellular	ADJ
mkg-997	36	11	enzyme	enzyme	NOUN
mkg-997	36	12	,	,	PUNCT
mkg-997	36	13	and	and	CCONJ
mkg-997	36	14	to	to	PART
mkg-997	36	15	suppress	suppress	VERB
mkg-997	36	16	mmp	mmp	PROPN
mkg-997	36	17	production	production	NOUN
mkg-997	36	18	,	,	PUNCT
mkg-997	36	19	to	to	PART
mkg-997	36	20	prevent	prevent	VERB
mkg-997	36	21	the	the	DET
mkg-997	36	22	formation	formation	NOUN
mkg-997	36	23	of	of	ADP
mkg-997	36	24	scar	scar	ADJ
mkg-997	36	25	tissue.9	tissue.9	NOUN
mkg-997	36	26	the	the	DET
mkg-997	36	27	study	study	NOUN
mkg-997	36	28	was	be	AUX
mkg-997	36	29	conducted	conduct	VERB
mkg-997	36	30	to	to	PART
mkg-997	36	31	analyze	analyze	VERB
mkg-997	36	32	the	the	DET
mkg-997	36	33	character	character	NOUN
mkg-997	36	34	of	of	ADP
mkg-997	36	35	amniotic	amniotic	ADJ
mkg-997	36	36	membrane	membrane	NOUN
mkg-997	36	37	slpi	slpi	NOUN
mkg-997	36	38	molecular	molecular	ADJ
mkg-997	36	39	protein	protein	NOUN
mkg-997	36	40	and	and	CCONJ
mkg-997	36	41	cloning	cloning	NOUN
mkg-997	36	42	of	of	ADP
mkg-997	36	43	amniotic	amniotic	ADJ
mkg-997	36	44	membrane	membrane	NOUN
mkg-997	36	45	slpi	slpi	NOUN
mkg-997	36	46	protein	protein	NOUN
mkg-997	36	47	to	to	PART
mkg-997	36	48	achieve	achieve	VERB
mkg-997	36	49	amniotic	amniotic	ADJ
mkg-997	36	50	membrane	membrane	NOUN
mkg-997	36	51	slpi	slpi	NOUN
mkg-997	36	52	protein	protein	NOUN
mkg-997	36	53	recombinant	recombinant	ADJ
mkg-997	36	54	in	in	ADP
mkg-997	36	55	large	large	ADJ
mkg-997	36	56	amount	amount	NOUN
mkg-997	36	57	which	which	PRON
mkg-997	36	58	would	would	AUX
mkg-997	36	59	be	be	AUX
mkg-997	36	60	beneficial	beneficial	ADJ
mkg-997	36	61	as	as	ADP
mkg-997	36	62	biomaterial	biomaterial	ADJ
mkg-997	36	63	candidate	candidate	NOUN
mkg-997	36	64	to	to	PART
mkg-997	36	65	achieve	achieve	VERB
mkg-997	36	66	fast	fast	ADJ
mkg-997	36	67	recovery	recovery	NOUN
mkg-997	36	68	of	of	ADP
mkg-997	36	69	gingival	gingival	NOUN
mkg-997	36	70	injury	injury	NOUN
mkg-997	36	71	.	.	PUNCT
mkg-997	37	1	material	material	NOUN
mkg-997	37	2	and	and	CCONJ
mkg-997	37	3	method	method	NOUN
mkg-997	37	4	identification	identification	NOUN
mkg-997	37	5	of	of	ADP
mkg-997	37	6	amniotic	amniotic	ADJ
mkg-997	37	7	membrane	membrane	NOUN
mkg-997	37	8	slpi	slpi	NOUN
mkg-997	37	9	gene	gene	NOUN
mkg-997	37	10	used	use	VERB
mkg-997	37	11	the	the	DET
mkg-997	37	12	following	follow	VERB
mkg-997	37	13	materials	material	NOUN
mkg-997	37	14	:	:	PUNCT
mkg-997	37	15	amniotic	amniotic	ADJ
mkg-997	37	16	membrane	membrane	NOUN
mkg-997	37	17	,	,	PUNCT
mkg-997	37	18	proteinase	proteinase	NOUN
mkg-997	37	19	k	k	PROPN
mkg-997	37	20	,	,	PUNCT
mkg-997	37	21	rnase	rnase	PROPN
mkg-997	37	22	,	,	PUNCT
mkg-997	37	23	rna	rna	PROPN
mkg-997	37	24	later	later	ADV
mkg-997	37	25	,	,	PUNCT
mkg-997	37	26	tbe	tbe	VERB
mkg-997	37	27	buffer	buffer	NOUN
mkg-997	37	28	,	,	PUNCT
mkg-997	37	29	ethidhium	ethidhium	NOUN
mkg-997	37	30	bromide	bromide	NOUN
mkg-997	37	31	,	,	PUNCT
mkg-997	37	32	bromophenol	bromophenol	NOUN
mkg-997	37	33	blue	blue	NOUN
mkg-997	37	34	,	,	PUNCT
mkg-997	37	35	marker	marker	NOUN
mkg-997	37	36	x	x	SYM
mkg-997	37	37	174x174	174x174	PROPN
mkg-997	38	1	rf	rf	ADJ
mkg-997	38	2	dna	dna	NOUN
mkg-997	38	3	/	/	SYM
mkg-997	38	4	hae	hae	PROPN
mkg-997	38	5	iii	iii	PROPN
mkg-997	38	6	fragments	fragment	NOUN
mkg-997	38	7	rnaa	rnaa	PROPN
mkg-997	38	8	later	later	ADV
mkg-997	38	9	,	,	PUNCT
mkg-997	38	10	rt	rt	NOUN
mkg-997	38	11	buffer	buffer	NOUN
mkg-997	38	12	,	,	PUNCT
mkg-997	38	13	ribonuclease	ribonuclease	NOUN
mkg-997	38	14	,	,	PUNCT
mkg-997	38	15	random	random	ADJ
mkg-997	38	16	primer	primer	NOUN
mkg-997	38	17	,	,	PUNCT
mkg-997	38	18	reverse	reverse	ADJ
mkg-997	38	19	transcriptase	transcriptase	NOUN
mkg-997	38	20	,	,	PUNCT
mkg-997	38	21	2	2	NUM
mkg-997	38	22	%	%	NOUN
mkg-997	38	23	agarose	agarose	NOUN
mkg-997	38	24	,	,	PUNCT
mkg-997	38	25	ethidium	ethidium	NOUN
mkg-997	38	26	bromide	bromide	NOUN
mkg-997	38	27	,	,	PUNCT
mkg-997	38	28	loading	loading	NOUN
mkg-997	38	29	dye	dye	NOUN
mkg-997	38	30	,	,	PUNCT
mkg-997	38	31	primer	primer	NOUN
mkg-997	38	32	(	(	PUNCT
mkg-997	38	33	sense	sense	NOUN
mkg-997	38	34	):	):	PUNCT
mkg-997	38	35	5	5	NUM
mkg-997	38	36	’	'	PUNCT
mkg-997	38	37	act	act	NOUN
mkg-997	38	38	cct	cct	PROPN
mkg-997	38	39	gcc	gcc	NOUN
mkg-997	38	40	ttc	ttc	PROPN
mkg-997	38	41	acc	acc	PROPN
mkg-997	38	42	atg	atg	PROPN
mkg-997	38	43	aa	aa	PROPN
mkg-997	38	44	3	3	NUM
mkg-997	38	45	’	'	PUNCT
mkg-997	38	46	and	and	CCONJ
mkg-997	38	47	primer	primer	NOUN
mkg-997	38	48	(	(	PUNCT
mkg-997	38	49	anti	anti	ADJ
mkg-997	38	50	sense	sense	NOUN
mkg-997	38	51	)	)	PUNCT
mkg-997	38	52	5	5	NUM
mkg-997	38	53	’	'	PUNCT
mkg-997	38	54	att	att	PROPN
mkg-997	38	55	cga	cga	PROPN
mkg-997	38	56	tca	tca	PROPN
mkg-997	38	57	act	act	PROPN
mkg-997	38	58	gga	gga	PROPN
mkg-997	38	59	ctt	ctt	PROPN
mkg-997	38	60	3	3	NUM
mkg-997	38	61	’	'	PUNCT
mkg-997	38	62	(	(	PUNCT
mkg-997	38	63	invitogen	invitogen	NOUN
mkg-997	38	64	)	)	PUNCT
mkg-997	38	65	.	.	PUNCT
mkg-997	39	1	100	100	NUM
mkg-997	39	2	bp	bp	PROPN
mkg-997	39	3	dna	dna	PROPN
mkg-997	39	4	marker	marker	NOUN
mkg-997	39	5	(	(	PUNCT
mkg-997	39	6	fermentas	fermenta	NOUN
mkg-997	39	7	)	)	PUNCT
mkg-997	39	8	,	,	PUNCT
mkg-997	39	9	distilled	distil	VERB
mkg-997	39	10	water	water	NOUN
mkg-997	39	11	,	,	PUNCT
mkg-997	39	12	tth	tth	NOUN
mkg-997	39	13	buffer	buffer	NOUN
mkg-997	39	14	reverse	reverse	NOUN
mkg-997	39	15	,	,	PUNCT
mkg-997	39	16	cdna	cdna	PROPN
mkg-997	39	17	mineral	mineral	NOUN
mkg-997	39	18	oil	oil	NOUN
mkg-997	39	19	,	,	PUNCT
mkg-997	39	20	enzyme	enzyme	VERB
mkg-997	39	21	tth	tth	PROPN
mkg-997	39	22	dna	dna	PROPN
mkg-997	39	23	polymerase	polymerase	NOUN
mkg-997	39	24	,	,	PUNCT
mkg-997	39	25	binding	bind	VERB
mkg-997	39	26	buffer	buffer	NOUN
mkg-997	39	27	(	(	PUNCT
mkg-997	39	28	pb	pb	NOUN
mkg-997	39	29	)	)	PUNCT
mkg-997	39	30	,	,	PUNCT
mkg-997	39	31	washing	washing	NOUN
mkg-997	39	32	buffer	buffer	NOUN
mkg-997	39	33	(	(	PUNCT
mkg-997	39	34	buffer	buffer	VERB
mkg-997	39	35	pe	pe	PROPN
mkg-997	39	36	)	)	PUNCT
mkg-997	39	37	,	,	PUNCT
mkg-997	39	38	200	200	NUM
mkg-997	39	39	µl	µl	ADP
mkg-997	39	40	buffer	buffer	NOUN
mkg-997	39	41	pe	pe	ADP
mkg-997	39	42	,	,	PUNCT
mkg-997	39	43	elution	elution	NOUN
mkg-997	39	44	buffer	buffer	NOUN
mkg-997	39	45	(	(	PUNCT
mkg-997	39	46	eb	eb	PROPN
mkg-997	39	47	)	)	PUNCT
mkg-997	39	48	,	,	PUNCT
mkg-997	39	49	sodium	sodium	NOUN
mkg-997	39	50	acetate	acetate	NOUN
mkg-997	39	51	,	,	PUNCT
mkg-997	39	52	100	100	NUM
mkg-997	39	53	%	%	NOUN
mkg-997	39	54	alcohol	alcohol	NOUN
mkg-997	39	55	,	,	PUNCT
mkg-997	39	56	70	70	NUM
mkg-997	39	57	%	%	NOUN
mkg-997	39	58	alcohol	alcohol	NOUN
mkg-997	39	59	,	,	PUNCT
mkg-997	39	60	pure	pure	ADJ
mkg-997	39	61	dna	dna	NOUN
mkg-997	39	62	,	,	PUNCT
mkg-997	39	63	template	template	NOUN
mkg-997	39	64	suppression	suppression	NOUN
mkg-997	39	65	reagent	reagent	NOUN
mkg-997	39	66	.	.	PUNCT
mkg-997	40	1	amplification	amplification	NOUN
mkg-997	40	2	,	,	PUNCT
mkg-997	40	3	purification	purification	NOUN
mkg-997	40	4	and	and	CCONJ
mkg-997	40	5	sequencing	sequencing	NOUN
mkg-997	40	6	of	of	ADP
mkg-997	40	7	amniotic	amniotic	ADJ
mkg-997	40	8	membrane	membrane	NOUN
mkg-997	40	9	slpi	slpi	NOUN
mkg-997	40	10	gene	gene	NOUN
mkg-997	40	11	using	use	VERB
mkg-997	40	12	the	the	DET
mkg-997	40	13	following	follow	VERB
mkg-997	40	14	materials	material	NOUN
mkg-997	40	15	:	:	PUNCT
mkg-997	40	16	amniotic	amniotic	ADJ
mkg-997	40	17	membrane	membrane	NOUN
mkg-997	40	18	slpi	slpi	NOUN
mkg-997	40	19	,	,	PUNCT
mkg-997	40	20	primer	primer	NOUN
mkg-997	40	21	:	:	PUNCT
mkg-997	40	22	5	5	NUM
mkg-997	40	23	’	'	PUNCT
mkg-997	40	24	cac	cac	PROPN
mkg-997	40	25	cat	cat	PROPN
mkg-997	40	26	gaa	gaa	PROPN
mkg-997	40	27	gtc	gtc	PROPN
mkg-997	40	28	cag	cag	PROPN
mkg-997	40	29	cgg	cgg	PROPN
mkg-997	40	30	cct	cct	PROPN
mkg-997	40	31	ctt	ctt	PROPN
mkg-997	40	32	cc	cc	PROPN
mkg-997	40	33	3	3	NUM
mkg-997	40	34	’	'	PUNCT
mkg-997	40	35	and	and	CCONJ
mkg-997	40	36	5	5	NUM
mkg-997	40	37	’	'	PUNCT
mkg-997	40	38	agc	agc	PROPN
mkg-997	40	39	ttt	ttt	PROPN
mkg-997	40	40	cac	cac	PROPN
mkg-997	40	41	agg	agg	PROPN
mkg-997	40	42	gga	gga	PROPN
mkg-997	40	43	aac	aac	PROPN
mkg-997	40	44	gca	gca	PROPN
mkg-997	40	45	ggat	ggat	ADJ
mkg-997	40	46	3	3	NUM
mkg-997	40	47	’	'	PUNCT
mkg-997	40	48	,	,	PUNCT
mkg-997	40	49	rneasy	rneasy	NOUN
mkg-997	40	50	mini	mini	NOUN
mkg-997	40	51	kit	kit	NOUN
mkg-997	40	52	(	(	PUNCT
mkg-997	40	53	qiagen	qiagen	PROPN
mkg-997	40	54	)	)	PUNCT
mkg-997	40	55	,	,	PUNCT
mkg-997	40	56	100	100	NUM
mkg-997	40	57	pb	pb	ADP
mkg-997	40	58	dna	dna	PROPN
mkg-997	40	59	marker	marker	NOUN
mkg-997	40	60	,	,	PUNCT
mkg-997	40	61	nuclease	nuclease	NOUN
mkg-997	40	62	free	free	ADJ
mkg-997	40	63	water	water	NOUN
mkg-997	40	64	(	(	PUNCT
mkg-997	40	65	mp	mp	PROPN
mkg-997	40	66	bio	bio	PROPN
mkg-997	40	67	)	)	PUNCT
mkg-997	40	68	,	,	PUNCT
mkg-997	40	69	ethidhium	ethidhium	NOUN
mkg-997	40	70	bromide	bromide	NOUN
mkg-997	40	71	(	(	PUNCT
mkg-997	40	72	mp	mp	PROPN
mkg-997	40	73	bio	bio	PROPN
mkg-997	40	74	)	)	PUNCT
mkg-997	40	75	,	,	PUNCT
mkg-997	40	76	agarose	agarose	NOUN
mkg-997	40	77	(	(	PUNCT
mkg-997	40	78	fermentas	fermenta	NOUN
mkg-997	40	79	)	)	PUNCT
mkg-997	40	80	,	,	PUNCT
mkg-997	40	81	6x	6x	NUM
mkg-997	40	82	loading	load	VERB
mkg-997	40	83	dye	dye	NOUN
mkg-997	40	84	solution	solution	NOUN
mkg-997	40	85	(	(	PUNCT
mkg-997	40	86	fermentas	fermenta	NOUN
mkg-997	40	87	)	)	PUNCT
mkg-997	40	88	,	,	PUNCT
mkg-997	40	89	tris	tris	PROPN
mkg-997	40	90	bufferc	bufferc	PROPN
mkg-997	40	91	edta	edta	PROPN
mkg-997	40	92	(	(	PUNCT
mkg-997	40	93	tbe	tbe	ADJ
mkg-997	40	94	)	)	PUNCT
mkg-997	40	95	buffer	buffer	VERB
mkg-997	40	96	10×	10×	NUM
mkg-997	40	97	ilt	ilt	NOUN
mkg-997	40	98	(	(	PUNCT
mkg-997	40	99	mp	mp	PROPN
mkg-997	40	100	bio	bio	PROPN
mkg-997	40	101	)	)	PUNCT
mkg-997	40	102	,	,	PUNCT
mkg-997	40	103	gel	gel	NOUN
mkg-997	40	104	extraction	extraction	NOUN
mkg-997	40	105	purification	purification	NOUN
mkg-997	40	106	kit	kit	NOUN
mkg-997	40	107	(	(	PUNCT
mkg-997	40	108	qiagen	qiagen	PROPN
mkg-997	40	109	)	)	PUNCT
mkg-997	40	110	,	,	PUNCT
mkg-997	40	111	low	low	ADJ
mkg-997	40	112	melting	melting	NOUN
mkg-997	40	113	agar	agar	NOUN
mkg-997	40	114	(	(	PUNCT
mkg-997	40	115	ssigma	ssigma	NOUN
mkg-997	40	116	)	)	PUNCT
mkg-997	40	117	,	,	PUNCT
mkg-997	40	118	dna	dna	NOUN
mkg-997	40	119	sequencing	sequencing	NOUN
mkg-997	40	120	kit	kit	NOUN
mkg-997	40	121	.	.	PUNCT
mkg-997	41	1	rna	rna	NOUN
mkg-997	41	2	isolation	isolation	NOUN
mkg-997	41	3	method	method	NOUN
mkg-997	41	4	as	as	ADP
mkg-997	41	5	the	the	DET
mkg-997	41	6	following	following	NOUN
mkg-997	41	7	:	:	PUNCT
mkg-997	41	8	3	3	NUM
mkg-997	41	9	×	×	NOUN
mkg-997	41	10	3	3	NUM
mkg-997	41	11	mm	mm	NOUN
mkg-997	41	12	(	(	PUNCT
mkg-997	41	13	30	30	NUM
mkg-997	41	14	µl	µl	NOUN
mkg-997	41	15	)	)	PUNCT
mkg-997	41	16	fresh	fresh	ADJ
mkg-997	41	17	amniotic	amniotic	ADJ
mkg-997	41	18	membrane	membrane	NOUN
mkg-997	41	19	sample	sample	NOUN
mkg-997	41	20	was	be	AUX
mkg-997	41	21	put	put	VERB
mkg-997	41	22	into	into	ADP
mkg-997	41	23	rna	rna	NOUN
mkg-997	41	24	later	later	ADV
mkg-997	41	25	and	and	CCONJ
mkg-997	41	26	kept	keep	VERB
mkg-997	41	27	at	at	ADP
mkg-997	41	28	40	40	NUM
mkg-997	41	29	°	°	NOUN
mkg-997	41	30	c	c	NOUN
mkg-997	41	31	in	in	ADP
mkg-997	41	32	refrigerator	refrigerator	NOUN
mkg-997	41	33	until	until	SCONJ
mkg-997	41	34	requested	request	VERB
mkg-997	41	35	.	.	PUNCT
mkg-997	42	1	30	30	NUM
mkg-997	42	2	µl	µl	ADP
mkg-997	42	3	amnion	amnion	NOUN
mkg-997	42	4	membrane	membrane	NOUN
mkg-997	42	5	sample	sample	NOUN
mkg-997	42	6	was	be	AUX
mkg-997	42	7	added	add	VERB
mkg-997	42	8	to	to	ADP
mkg-997	42	9	300	300	NUM
mkg-997	42	10	µl	µl	ADP
mkg-997	42	11	buffer	buffer	NOUN
mkg-997	42	12	rlt	rlt	NOUN
mkg-997	42	13	followed	follow	VERB
mkg-997	42	14	by	by	ADP
mkg-997	42	15	590	590	NUM
mkg-997	42	16	µl	µl	ADP
mkg-997	42	17	distilled	distil	VERB
mkg-997	42	18	water	water	NOUN
mkg-997	42	19	into	into	ADP
mkg-997	42	20	20µl	20µl	ADJ
mkg-997	42	21	eppendorf	eppendorf	PROPN
mkg-997	42	22	tube	tube	NOUN
mkg-997	42	23	,	,	PUNCT
mkg-997	42	24	then	then	ADV
mkg-997	42	25	,	,	PUNCT
mkg-997	42	26	10	10	NUM
mkg-997	42	27	µl	µl	ADP
mkg-997	42	28	proteinase	proteinase	PROPN
mkg-997	42	29	k	k	PROPN
mkg-997	42	30	was	be	AUX
mkg-997	42	31	added	add	VERB
mkg-997	42	32	.	.	PUNCT
mkg-997	43	1	the	the	DET
mkg-997	43	2	mixture	mixture	NOUN
mkg-997	43	3	was	be	AUX
mkg-997	43	4	incubated	incubate	VERB
mkg-997	43	5	at	at	ADP
mkg-997	43	6	55	55	NUM
mkg-997	43	7	°	°	ADP
mkg-997	43	8	c	c	PROPN
mkg-997	43	9	for	for	ADP
mkg-997	43	10	min	min	PROPN
mkg-997	43	11	.	.	PROPN
mkg-997	43	12	next	next	ADV
mkg-997	43	13	,	,	PUNCT
mkg-997	43	14	centrifugation	centrifugation	NOUN
mkg-997	43	15	was	be	AUX
mkg-997	43	16	done	do	VERB
mkg-997	43	17	in	in	ADP
mkg-997	43	18	3	3	NUM
mkg-997	43	19	min	min	NOUN
mkg-997	43	20	at	at	ADP
mkg-997	43	21	10.000	10.000	NUM
mkg-997	43	22	rpm	rpm	NOUN
mkg-997	43	23	at	at	ADP
mkg-997	43	24	20–25	20–25	NUM
mkg-997	43	25	°	°	ADJ
mkg-997	43	26	c.	c.	NOUN
mkg-997	43	27	1.5	1.5	NUM
mkg-997	43	28	µl	µl	ADP
mkg-997	43	29	other	other	ADJ
mkg-997	43	30	eppendorf	eppendorf	NOUN
mkg-997	43	31	was	be	AUX
mkg-997	43	32	filled	fill	VERB
mkg-997	43	33	with	with	ADP
mkg-997	43	34	900	900	NUM
mkg-997	43	35	µl	µl	ADP
mkg-997	43	36	supernatant	supernatant	NOUN
mkg-997	43	37	added	add	VERB
mkg-997	43	38	450	450	NUM
mkg-997	43	39	µl	µl	PART
mkg-997	43	40	(	(	PUNCT
mkg-997	43	41	1/2	1/2	NUM
mkg-997	43	42	vol	vol	NOUN
mkg-997	43	43	)	)	PUNCT
mkg-997	43	44	ethanol	ethanol	NOUN
mkg-997	43	45	100	100	NUM
mkg-997	43	46	%	%	NOUN
mkg-997	43	47	mixture	mixture	NOUN
mkg-997	43	48	of	of	ADP
mkg-997	43	49	sample	sample	NOUN
mkg-997	43	50	was	be	AUX
mkg-997	43	51	done	do	VERB
mkg-997	43	52	using	use	VERB
mkg-997	43	53	pipette	pipette	NOUN
mkg-997	43	54	into	into	ADP
mkg-997	43	55	rna	rna	NOUN
mkg-997	43	56	tube	tube	NOUN
mkg-997	43	57	,	,	PUNCT
mkg-997	43	58	centrifugation	centrifugation	NOUN
mkg-997	43	59	at	at	ADP
mkg-997	43	60	10.000	10.000	NUM
mkg-997	43	61	rpm	rpm	NOUN
mkg-997	43	62	,	,	PUNCT
mkg-997	43	63	the	the	DET
mkg-997	43	64	lower	low	ADJ
mkg-997	43	65	part	part	NOUN
mkg-997	43	66	of	of	ADP
mkg-997	43	67	the	the	DET
mkg-997	43	68	solution	solution	NOUN
mkg-997	43	69	was	be	AUX
mkg-997	43	70	evacuated	evacuate	VERB
mkg-997	43	71	.	.	PUNCT
mkg-997	44	1	centrifugation	centrifugation	NOUN
mkg-997	44	2	was	be	AUX
mkg-997	44	3	repeated	repeat	VERB
mkg-997	44	4	for	for	ADP
mkg-997	44	5	15	15	NUM
mkg-997	44	6	second	second	NOUN
mkg-997	44	7	at	at	ADP
mkg-997	44	8	10.000	10.000	NUM
mkg-997	44	9	rpm	rpm	NOUN
mkg-997	44	10	,	,	PUNCT
mkg-997	44	11	lower	low	ADJ
mkg-997	44	12	part	part	NOUN
mkg-997	44	13	of	of	ADP
mkg-997	44	14	the	the	DET
mkg-997	44	15	solution	solution	NOUN
mkg-997	44	16	evacuated	evacuate	VERB
mkg-997	44	17	added	add	VERB
mkg-997	44	18	by	by	ADP
mkg-997	44	19	700	700	NUM
mkg-997	44	20	µl	µl	NOUN
mkg-997	44	21	buffer	buffer	NOUN
mkg-997	44	22	rw	rw	NOUN
mkg-997	44	23	,	,	PUNCT
mkg-997	44	24	centrifuged	centrifuge	VERB
mkg-997	44	25	for	for	ADP
mkg-997	44	26	15	15	NUM
mkg-997	44	27	second	second	NOUN
mkg-997	44	28	at	at	ADP
mkg-997	44	29	10.000	10.000	NUM
mkg-997	44	30	rpm	rpm	NOUN
mkg-997	44	31	.	.	PUNCT
mkg-997	45	1	new	new	ADJ
mkg-997	45	2	collective	collective	ADJ
mkg-997	45	3	tube	tube	NOUN
mkg-997	45	4	was	be	AUX
mkg-997	45	5	filled	fill	VERB
mkg-997	45	6	by	by	ADP
mkg-997	45	7	500	500	NUM
mkg-997	45	8	µl	µl	NOUN
mkg-997	45	9	buffer	buffer	NOUN
mkg-997	45	10	rpe	rpe	PROPN
mkg-997	45	11	,	,	PUNCT
mkg-997	45	12	following	follow	VERB
mkg-997	45	13	by	by	ADP
mkg-997	45	14	2	2	NUM
mkg-997	45	15	minute	minute	NOUN
mkg-997	45	16	10.000	10.000	NUM
mkg-997	45	17	rpm	rpm	NOUN
mkg-997	45	18	centrifugation	centrifugation	NOUN
mkg-997	45	19	,	,	PUNCT
mkg-997	45	20	repeated	repeat	VERB
mkg-997	45	21	to	to	PART
mkg-997	45	22	throw	throw	VERB
mkg-997	45	23	out	out	ADP
mkg-997	45	24	the	the	DET
mkg-997	45	25	lower	low	ADJ
mkg-997	45	26	part	part	NOUN
mkg-997	45	27	of	of	ADP
mkg-997	45	28	the	the	DET
mkg-997	45	29	solution	solution	NOUN
mkg-997	45	30	.	.	PUNCT
mkg-997	46	1	1.5	1.5	NUM
mkg-997	46	2	ml	ml	ADP
mkg-997	46	3	eppendorf	eppendorf	PROPN
mkg-997	46	4	tube	tube	NOUN
mkg-997	46	5	was	be	AUX
mkg-997	46	6	filled	fill	VERB
mkg-997	46	7	by	by	ADP
mkg-997	46	8	50	50	NUM
mkg-997	46	9	µl	µl	ADP
mkg-997	46	10	rneasy	rneasy	NOUN
mkg-997	46	11	free	free	ADJ
mkg-997	46	12	water	water	NOUN
mkg-997	46	13	,	,	PUNCT
mkg-997	46	14	then	then	ADV
mkg-997	46	15	centrifugation	centrifugation	NOUN
mkg-997	46	16	was	be	AUX
mkg-997	46	17	done	do	VERB
mkg-997	46	18	was	be	AUX
mkg-997	46	19	done	do	VERB
mkg-997	46	20	in	in	ADP
mkg-997	46	21	1	1	NUM
mkg-997	46	22	minute	minute	NOUN
mkg-997	46	23	at	at	ADP
mkg-997	46	24	10.000	10.000	NUM
mkg-997	46	25	rpm	rpm	NOUN
mkg-997	46	26	.	.	PUNCT
mkg-997	47	1	the	the	DET
mkg-997	47	2	result	result	NOUN
mkg-997	47	3	of	of	ADP
mkg-997	47	4	the	the	DET
mkg-997	47	5	procedure	procedure	NOUN
mkg-997	47	6	was	be	AUX
mkg-997	47	7	rna	rna	NOUN
mkg-997	47	8	continued	continue	VERB
mkg-997	47	9	by	by	ADP
mkg-997	47	10	cdna	cdna	PROPN
mkg-997	47	11	isolation	isolation	NOUN
mkg-997	47	12	.	.	PUNCT
mkg-997	48	1	isolation	isolation	NOUN
mkg-997	48	2	of	of	ADP
mkg-997	48	3	cdna	cdna	PROPN
mkg-997	48	4	using	use	VERB
mkg-997	48	5	the	the	DET
mkg-997	48	6	following	follow	VERB
mkg-997	48	7	procedures	procedure	NOUN
mkg-997	48	8	:	:	PUNCT
mkg-997	48	9	10µl	10µl	ADJ
mkg-997	48	10	rna	rna	NOUN
mkg-997	48	11	was	be	AUX
mkg-997	48	12	incubated	incubate	VERB
mkg-997	48	13	at	at	ADP
mkg-997	48	14	65	65	NUM
mkg-997	48	15	°	°	NOUN
mkg-997	48	16	c	c	NOUN
mkg-997	48	17	for	for	ADP
mkg-997	48	18	3	3	NUM
mkg-997	48	19	minute	minute	NOUN
mkg-997	48	20	,	,	PUNCT
mkg-997	48	21	added	add	VERB
mkg-997	48	22	by	by	ADP
mkg-997	48	23	4µl	4µl	ADJ
mkg-997	48	24	(	(	PUNCT
mkg-997	48	25	5x	5x	NUM
mkg-997	48	26	)	)	PUNCT
mkg-997	48	27	buffer	buffer	NOUN
mkg-997	48	28	rt	rt	PROPN
mkg-997	48	29	,	,	PUNCT
mkg-997	48	30	4	4	NUM
mkg-997	48	31	µl	µl	ADP
mkg-997	48	32	dntps	dntps	ADJ
mkg-997	48	33	mix	mix	NOUN
mkg-997	48	34	,	,	PUNCT
mkg-997	48	35	0.5µl	0.5µl	NUM
mkg-997	48	36	ribonuclease	ribonuclease	NOUN
mkg-997	48	37	,	,	PUNCT
mkg-997	48	38	0.5µl	0.5µl	NUM
mkg-997	48	39	random	random	ADJ
mkg-997	48	40	primer	primer	NOUN
mkg-997	48	41	,	,	PUNCT
mkg-997	48	42	0.5	0.5	NUM
mkg-997	48	43	µl	µl	ADP
mkg-997	48	44	reverse	reverse	ADJ
mkg-997	48	45	transcriptase	transcriptase	NOUN
mkg-997	48	46	(	(	PUNCT
mkg-997	48	47	19.5	19.5	NUM
mkg-997	48	48	µl	µl	NOUN
mkg-997	48	49	)	)	PUNCT
mkg-997	48	50	.	.	PUNCT
mkg-997	49	1	the	the	DET
mkg-997	49	2	mixture	mixture	NOUN
mkg-997	49	3	was	be	AUX
mkg-997	49	4	incubated	incubate	VERB
mkg-997	49	5	at	at	ADP
mkg-997	49	6	42	42	NUM
mkg-997	49	7	°	°	NOUN
mkg-997	49	8	c	c	NOUN
mkg-997	49	9	for	for	ADP
mkg-997	49	10	1	1	NUM
mkg-997	49	11	hour	hour	NOUN
mkg-997	49	12	.	.	PUNCT
mkg-997	50	1	electrophoresis	electrophoresis	NOUN
mkg-997	50	2	was	be	AUX
mkg-997	50	3	performed	perform	VERB
mkg-997	50	4	to	to	PART
mkg-997	50	5	identify	identify	VERB
mkg-997	50	6	whether	whether	SCONJ
mkg-997	50	7	cdna	cdna	PROPN
mkg-997	50	8	was	be	AUX
mkg-997	50	9	formed	form	VERB
mkg-997	50	10	in	in	ADP
mkg-997	50	11	2	2	NUM
mkg-997	50	12	%	%	NOUN
mkg-997	50	13	agarose	agarose	NOUN
mkg-997	50	14	gel	gel	NOUN
mkg-997	50	15	containing	contain	VERB
mkg-997	50	16	ethidium	ethidium	ADJ
mkg-997	50	17	bromide	bromide	NOUN
mkg-997	50	18	.	.	PUNCT
mkg-997	51	1	5	5	NUM
mkg-997	51	2	µl	µl	ADP
mkg-997	51	3	cdna	cdna	PROPN
mkg-997	51	4	was	be	AUX
mkg-997	51	5	added	add	VERB
mkg-997	51	6	by	by	ADP
mkg-997	51	7	2	2	NUM
mkg-997	51	8	µl	µl	ADP
mkg-997	51	9	loading	loading	NOUN
mkg-997	51	10	dye	dye	NOUN
mkg-997	51	11	and	and	CCONJ
mkg-997	51	12	put	put	VERB
mkg-997	51	13	into	into	ADP
mkg-997	51	14	well	well	ADV
mkg-997	51	15	agarose	agarose	NOUN
mkg-997	51	16	,	,	PUNCT
mkg-997	51	17	then	then	ADV
mkg-997	51	18	,	,	PUNCT
mkg-997	51	19	it	it	PRON
mkg-997	51	20	was	be	AUX
mkg-997	51	21	run	run	VERB
mkg-997	51	22	in	in	ADP
mkg-997	51	23	100	100	NUM
mkg-997	51	24	volt	volt	NOUN
mkg-997	51	25	for	for	ADP
mkg-997	51	26	±	±	NUM
mkg-997	51	27	30	30	NUM
mkg-997	51	28	minute	minute	NOUN
mkg-997	51	29	,	,	PUNCT
mkg-997	51	30	followed	follow	VERB
mkg-997	51	31	by	by	ADP
mkg-997	51	32	detection	detection	NOUN
mkg-997	51	33	with	with	ADP
mkg-997	51	34	uv	uv	NOUN
mkg-997	51	35	-	-	PUNCT
mkg-997	51	36	transilluminator	transilluminator	NOUN
mkg-997	51	37	and	and	CCONJ
mkg-997	51	38	documented	document	VERB
mkg-997	51	39	by	by	ADP
mkg-997	51	40	polaroid	polaroid	PROPN
mkg-997	51	41	camera	camera	PROPN
mkg-997	51	42	.	.	PUNCT
mkg-997	52	1	polymerase	polymerase	NOUN
mkg-997	52	2	chain	chain	NOUN
mkg-997	52	3	reaction	reaction	NOUN
mkg-997	52	4	(	(	PUNCT
mkg-997	52	5	pcr	pcr	PROPN
mkg-997	52	6	)	)	PUNCT
mkg-997	52	7	was	be	AUX
mkg-997	52	8	done	do	VERB
mkg-997	52	9	in	in	ADP
mkg-997	52	10	the	the	DET
mkg-997	52	11	following	follow	VERB
mkg-997	52	12	procedure	procedure	NOUN
mkg-997	52	13	:	:	PUNCT
mkg-997	52	14	70	70	NUM
mkg-997	52	15	µl	µl	ADP
mkg-997	52	16	distilled	distil	VERB
mkg-997	52	17	water	water	NOUN
mkg-997	52	18	mixed	mix	VERB
mkg-997	52	19	with	with	ADP
mkg-997	52	20	9	9	NUM
mkg-997	52	21	µl	µl	NOUN
mkg-997	52	22	buffer	buffer	NOUN
mkg-997	52	23	reaction	reaction	NOUN
mkg-997	52	24	tth	tth	PROPN
mkg-997	52	25	,	,	PUNCT
mkg-997	52	26	8	8	NUM
mkg-997	52	27	µl	µl	ADP
mkg-997	52	28	dntp	dntp	NOUN
mkg-997	52	29	mix	mix	NOUN
mkg-997	52	30	,	,	PUNCT
mkg-997	52	31	1µl	1µl	ADJ
mkg-997	52	32	primer	primer	NOUN
mkg-997	52	33	reverse	reverse	VERB
mkg-997	52	34	cat	cat	NOUN
mkg-997	52	35	tcg	tcg	PROPN
mkg-997	52	36	atc	atc	PROPN
mkg-997	52	37	aac	aac	PROPN
mkg-997	52	38	tgg	tgg	PROPN
mkg-997	52	39	cac	cac	PROPN
mkg-997	52	40	tt	tt	PROPN
mkg-997	52	41	and	and	CCONJ
mkg-997	52	42	1	1	NUM
mkg-997	52	43	µl	µl	ADP
mkg-997	52	44	primer	primer	NOUN
mkg-997	52	45	forward	forward	ADV
mkg-997	52	46	act	act	PROPN
mkg-997	52	47	cct	cct	PROPN
mkg-997	52	48	gcc	gcc	NOUN
mkg-997	52	49	ttc	ttc	PROPN
mkg-997	52	50	acc	acc	PROPN
mkg-997	52	51	atg	atg	PROPN
mkg-997	52	52	aa	aa	PROPN
mkg-997	52	53	1	1	NUM
mkg-997	52	54	µl	µl	ADP
mkg-997	52	55	cdna	cdna	PROPN
mkg-997	52	56	(	(	PUNCT
mkg-997	52	57	total	total	ADJ
mkg-997	52	58	99	99	NUM
mkg-997	52	59	µl	µl	NOUN
mkg-997	52	60	)	)	PUNCT
mkg-997	52	61	,	,	PUNCT
mkg-997	52	62	next	next	ADV
mkg-997	52	63	,	,	PUNCT
mkg-997	52	64	added	add	VERB
mkg-997	52	65	by	by	ADP
mkg-997	52	66	102	102	NUM
mkg-997	52	67	µl	µl	ADP
mkg-997	52	68	mineral	mineral	NOUN
mkg-997	52	69	oil	oil	NOUN
mkg-997	52	70	denaturation	denaturation	NOUN
mkg-997	52	71	was	be	AUX
mkg-997	52	72	done	do	VERB
mkg-997	52	73	at	at	ADP
mkg-997	52	74	72	72	NUM
mkg-997	52	75	°	°	ADP
mkg-997	52	76	c	c	NOUN
mkg-997	52	77	for	for	ADP
mkg-997	52	78	30	30	NUM
mkg-997	52	79	second	second	ADJ
mkg-997	52	80	and	and	CCONJ
mkg-997	53	1	94	94	NUM
mkg-997	53	2	°	°	NOUN
mkg-997	53	3	c	c	NOUN
mkg-997	53	4	for	for	ADP
mkg-997	53	5	5	5	NUM
mkg-997	53	6	minutes	minute	NOUN
mkg-997	53	7	,	,	PUNCT
mkg-997	53	8	continued	continue	VERB
mkg-997	53	9	by	by	ADP
mkg-997	53	10	adding	add	VERB
mkg-997	53	11	1	1	NUM
mkg-997	53	12	µl	µl	ADP
mkg-997	53	13	tth	tth	PROPN
mkg-997	53	14	dna	dna	PROPN
mkg-997	53	15	polymerase	polymerase	NOUN
mkg-997	53	16	,	,	PUNCT
mkg-997	53	17	then	then	ADV
mkg-997	53	18	inserte	inserte	NOUN
mkg-997	53	19	d	d	X
mkg-997	53	20	into	into	ADP
mkg-997	53	21	pcr	pcr	ADJ
mkg-997	53	22	machine	machine	NOUN
mkg-997	53	23	.	.	PUNCT
mkg-997	54	1	initially	initially	ADV
mkg-997	54	2	denaturation	denaturation	PROPN
mkg-997	54	3	was	be	AUX
mkg-997	54	4	performed	perform	VERB
mkg-997	54	5	at	at	ADP
mkg-997	54	6	940	940	NUM
mkg-997	54	7	c	c	NOUN
mkg-997	54	8	for	for	ADP
mkg-997	54	9	40	40	NUM
mkg-997	54	10	second	second	ADJ
mkg-997	54	11	,	,	PUNCT
mkg-997	54	12	annealing	anneal	VERB
mkg-997	54	13	at	at	ADP
mkg-997	54	14	52	52	NUM
mkg-997	54	15	°	°	NOUN
mkg-997	54	16	c	c	NOUN
mkg-997	54	17	for	for	ADP
mkg-997	54	18	40	40	NUM
mkg-997	54	19	second	second	ADJ
mkg-997	54	20	,	,	PUNCT
mkg-997	54	21	extension	extension	NOUN
mkg-997	54	22	was	be	AUX
mkg-997	54	23	done	do	VERB
mkg-997	54	24	at	at	ADP
mkg-997	54	25	72	72	NUM
mkg-997	54	26	°	°	ADP
mkg-997	54	27	c	c	NOUN
mkg-997	54	28	for	for	ADP
mkg-997	54	29	40	40	NUM
mkg-997	54	30	second	second	ADJ
mkg-997	54	31	,	,	PUNCT
mkg-997	54	32	extra	extra	ADJ
mkg-997	54	33	extension	extension	NOUN
mkg-997	54	34	at	at	ADP
mkg-997	54	35	72	72	NUM
mkg-997	54	36	°	°	ADP
mkg-997	54	37	c	c	NOUN
mkg-997	54	38	for	for	ADP
mkg-997	54	39	10	10	NUM
mkg-997	54	40	mm	mm	NOUN
mkg-997	54	41	.	.	PUNCT
mkg-997	55	1	pcr	pcr	PROPN
mkg-997	55	2	was	be	AUX
mkg-997	55	3	done	do	VERB
mkg-997	55	4	in	in	ADP
mkg-997	55	5	35	35	NUM
mkg-997	55	6	cycles	cycle	NOUN
mkg-997	55	7	analysis	analysis	NOUN
mkg-997	55	8	of	of	ADP
mkg-997	55	9	pcr	pcr	ADJ
mkg-997	55	10	product	product	NOUN
mkg-997	55	11	with	with	ADP
mkg-997	55	12	electrophoresis	electrophoresis	NOUN
mkg-997	55	13	agarose	agarose	NOUN
mkg-997	55	14	gel	gel	NOUN
mkg-997	55	15	.	.	PUNCT
mkg-997	56	1	the	the	DET
mkg-997	56	2	result	result	NOUN
mkg-997	56	3	of	of	ADP
mkg-997	56	4	pcr	pcr	NOUN
mkg-997	56	5	could	could	AUX
mkg-997	56	6	be	be	AUX
mkg-997	56	7	seen	see	VERB
mkg-997	56	8	through	through	ADP
mkg-997	56	9	electrophoresis	electrophoresis	NOUN
mkg-997	56	10	in	in	ADP
mkg-997	56	11	2	2	NUM
mkg-997	56	12	%	%	NOUN
mkg-997	56	13	agarose	agarose	NOUN
mkg-997	56	14	gel	gel	NOUN
mkg-997	56	15	containing	contain	VERB
mkg-997	56	16	ethidhium	ethidhium	NOUN
mkg-997	56	17	bromide	bromide	NOUN
mkg-997	56	18	.	.	PUNCT
mkg-997	57	1	5	5	NUM
mkg-997	57	2	µl	µl	ADP
mkg-997	57	3	cdna	cdna	PROPN
mkg-997	57	4	added	add	VERB
mkg-997	57	5	by	by	ADP
mkg-997	57	6	2	2	NUM
mkg-997	57	7	µl	µl	ADP
mkg-997	57	8	loading	loading	NOUN
mkg-997	57	9	dye	dye	NOUN
mkg-997	57	10	put	put	VERB
mkg-997	57	11	into	into	ADP
mkg-997	57	12	well	well	ADV
mkg-997	57	13	agarose	agarose	NOUN
mkg-997	57	14	.	.	PUNCT
mkg-997	58	1	the	the	DET
mkg-997	58	2	mixture	mixture	NOUN
mkg-997	58	3	was	be	AUX
mkg-997	58	4	run	run	VERB
mkg-997	58	5	in	in	ADP
mkg-997	58	6	100	100	NUM
mkg-997	58	7	volt	volt	NOUN
mkg-997	58	8	for	for	ADP
mkg-997	58	9	30	30	NUM
mkg-997	58	10	min	min	NOUN
mkg-997	58	11	.	.	PROPN
mkg-997	58	12	next	next	ADJ
mkg-997	58	13	,	,	PUNCT
mkg-997	58	14	detection	detection	NOUN
mkg-997	58	15	was	be	AUX
mkg-997	58	16	conducted	conduct	VERB
mkg-997	58	17	by	by	ADP
mkg-997	58	18	uv	uv	NOUN
mkg-997	58	19	-	-	PUNCT
mkg-997	58	20	transuminator	transuminator	NOUN
mkg-997	58	21	,	,	PUNCT
mkg-997	58	22	then	then	ADV
mkg-997	58	23	,	,	PUNCT
mkg-997	58	24	the	the	DET
mkg-997	58	25	result	result	NOUN
mkg-997	58	26	was	be	AUX
mkg-997	58	27	documented	document	VERB
mkg-997	58	28	using	use	VERB
mkg-997	58	29	polaroid	polaroid	PROPN
mkg-997	58	30	camera	camera	NOUN
mkg-997	58	31	.	.	PUNCT
mkg-997	59	1	purification	purification	NOUN
mkg-997	59	2	of	of	ADP
mkg-997	59	3	pcr	pcr	ADJ
mkg-997	59	4	product	product	NOUN
mkg-997	59	5	was	be	AUX
mkg-997	59	6	performed	perform	VERB
mkg-997	59	7	using	use	VERB
mkg-997	59	8	purification	purification	NOUN
mkg-997	59	9	kit	kit	NOUN
mkg-997	59	10	(	(	PUNCT
mkg-997	59	11	qiagen	qiagen	PROPN
mkg-997	59	12	)	)	PUNCT
mkg-997	59	13	.	.	PUNCT
mkg-997	60	1	100µl	100µl	ADJ
mkg-997	60	2	pcr	pcr	ADJ
mkg-997	60	3	product	product	NOUN
mkg-997	60	4	was	be	AUX
mkg-997	60	5	added	add	VERB
mkg-997	60	6	by	by	ADP
mkg-997	60	7	500	500	NUM
mkg-997	60	8	µl	µl	AUX
mkg-997	60	9	buffer	buffer	NOUN
mkg-997	60	10	binding	bind	VERB
mkg-997	60	11	(	(	PUNCT
mkg-997	60	12	pb	pb	NOUN
mkg-997	60	13	)	)	PUNCT
mkg-997	60	14	and	and	CCONJ
mkg-997	60	15	mixed	mix	VERB
mkg-997	60	16	to	to	PART
mkg-997	60	17	release	release	VERB
mkg-997	60	18	mineral	mineral	NOUN
mkg-997	60	19	oil	oil	NOUN
mkg-997	60	20	.	.	PUNCT
mkg-997	61	1	the	the	DET
mkg-997	61	2	mixture	mixture	NOUN
mkg-997	61	3	was	be	AUX
mkg-997	61	4	moved	move	VERB
mkg-997	61	5	into	into	ADP
mkg-997	61	6	column	column	NOUN
mkg-997	61	7	,	,	PUNCT
mkg-997	61	8	nest	nest	NOUN
mkg-997	61	9	step	step	NOUN
mkg-997	61	10	,	,	PUNCT
mkg-997	61	11	centrifugation	centrifugation	NOUN
mkg-997	61	12	was	be	AUX
mkg-997	61	13	performed	perform	VERB
mkg-997	61	14	at	at	ADP
mkg-997	61	15	14.000	14.000	NUM
mkg-997	61	16	rpm	rpm	NOUN
mkg-997	61	17	at	at	ADP
mkg-997	61	18	room	room	NOUN
mkg-997	61	19	temperature	temperature	NOUN
mkg-997	61	20	for	for	ADP
mkg-997	61	21	1minute	1minute	NUM
mkg-997	61	22	.	.	PUNCT
mkg-997	62	1	the	the	DET
mkg-997	62	2	solution	solution	NOUN
mkg-997	62	3	in	in	ADP
mkg-997	62	4	the	the	DET
mkg-997	62	5	lower	low	ADJ
mkg-997	62	6	part	part	NOUN
mkg-997	62	7	was	be	AUX
mkg-997	62	8	removed	remove	VERB
mkg-997	62	9	and	and	CCONJ
mkg-997	62	10	rinsed	rinse	VERB
mkg-997	62	11	by	by	ADP
mkg-997	62	12	750	750	NUM
mkg-997	62	13	µl	µl	ADP
mkg-997	62	14	washing	washing	NOUN
mkg-997	62	15	buffer	buffer	NOUN
mkg-997	62	16	,	,	PUNCT
mkg-997	62	17	the	the	DET
mkg-997	62	18	mixture	mixture	NOUN
mkg-997	62	19	was	be	AUX
mkg-997	62	20	centrifuged	centrifuge	VERB
mkg-997	62	21	at	at	ADP
mkg-997	62	22	14.000	14.000	NUM
mkg-997	62	23	rpm	rpm	NOUN
mkg-997	62	24	at	at	ADP
mkg-997	62	25	room	room	NOUN
mkg-997	62	26	temperature	temperature	NOUN
mkg-997	62	27	for	for	ADP
mkg-997	62	28	1	1	NUM
mkg-997	62	29	minute	minute	NOUN
mkg-997	62	30	,	,	PUNCT
mkg-997	62	31	the	the	DET
mkg-997	62	32	fluid	fluid	NOUN
mkg-997	62	33	was	be	AUX
mkg-997	62	34	thrown	throw	VERB
mkg-997	62	35	out	out	ADP
mkg-997	62	36	,	,	PUNCT
mkg-997	62	37	added	add	VERB
mkg-997	62	38	by	by	ADP
mkg-997	62	39	200µl	200µl	ADJ
mkg-997	62	40	buffer	buffer	NOUN
mkg-997	62	41	pb	pb	ADP
mkg-997	62	42	centrifugation	centrifugation	NOUN
mkg-997	62	43	was	be	AUX
mkg-997	62	44	performed	perform	VERB
mkg-997	62	45	again	again	ADV
mkg-997	62	46	at	at	ADP
mkg-997	62	47	14.000	14.000	NUM
mkg-997	62	48	rpm	rpm	NOUN
mkg-997	62	49	at	at	ADP
mkg-997	62	50	room	room	NOUN
mkg-997	62	51	temperature	temperature	NOUN
mkg-997	62	52	for	for	ADP
mkg-997	62	53	1	1	NUM
mkg-997	62	54	minute	minute	NOUN
mkg-997	62	55	.	.	PUNCT
mkg-997	63	1	pure	pure	ADJ
mkg-997	63	2	dna	dna	PROPN
mkg-997	63	3	which	which	PRON
mkg-997	63	4	was	be	AUX
mkg-997	63	5	purified	purify	VERB
mkg-997	63	6	put	put	VERB
mkg-997	63	7	into	into	ADP
mkg-997	63	8	eppendorf	eppendorf	NOUN
mkg-997	63	9	,	,	PUNCT
mkg-997	63	10	added	add	VERB
mkg-997	63	11	by	by	ADP
mkg-997	63	12	50	50	NUM
mkg-997	63	13	µl	µl	ADP
mkg-997	63	14	buffer	buffer	NOUN
mkg-997	63	15	elution	elution	NOUN
mkg-997	63	16	(	(	PUNCT
mkg-997	63	17	b.e	b.e	PROPN
mkg-997	63	18	)	)	PUNCT
mkg-997	63	19	and	and	CCONJ
mkg-997	63	20	it	it	PRON
mkg-997	63	21	was	be	AUX
mkg-997	63	22	left	leave	VERB
mkg-997	63	23	for	for	ADP
mkg-997	63	24	5	5	NUM
mkg-997	63	25	minute	minute	NOUN
mkg-997	63	26	.	.	PUNCT
mkg-997	64	1	centrifugation	centrifugation	NOUN
mkg-997	64	2	was	be	AUX
mkg-997	64	3	done	do	VERB
mkg-997	64	4	at	at	ADP
mkg-997	64	5	14.000	14.000	NUM
mkg-997	64	6	rpm	rpm	NOUN
mkg-997	64	7	at	at	ADP
mkg-997	64	8	room	room	NOUN
mkg-997	64	9	temperature	temperature	NOUN
mkg-997	64	10	for	for	ADP
mkg-997	64	11	1	1	NUM
mkg-997	64	12	min	min	NOUN
mkg-997	64	13	.	.	PROPN
mkg-997	64	14	electrophoresis	electrophoresis	NOUN
mkg-997	64	15	was	be	AUX
mkg-997	64	16	done	do	VERB
mkg-997	64	17	to	to	PART
mkg-997	64	18	identify	identify	VERB
mkg-997	64	19	the	the	DET
mkg-997	64	20	result	result	NOUN
mkg-997	64	21	of	of	ADP
mkg-997	64	22	dna	dna	PROPN
mkg-997	64	23	purification	purification	NOUN
mkg-997	64	24	in	in	ADP
mkg-997	64	25	2	2	NUM
mkg-997	64	26	%	%	NOUN
mkg-997	64	27	agarose	agarose	NOUN
mkg-997	64	28	gel	gel	NOUN
mkg-997	64	29	containing	contain	VERB
mkg-997	64	30	ethidhium	ethidhium	NOUN
mkg-997	64	31	bromide	bromide	NOUN
mkg-997	64	32	.	.	PUNCT
mkg-997	65	1	5	5	NUM
mkg-997	65	2	µl	µl	ADP
mkg-997	65	3	pcr	pcr	ADJ
mkg-997	65	4	product	product	NOUN
mkg-997	65	5	had	have	AUX
mkg-997	65	6	been	be	AUX
mkg-997	65	7	purified	purify	VERB
mkg-997	65	8	and	and	CCONJ
mkg-997	65	9	mixed	mixed	ADJ
mkg-997	65	10	125munadziroh	125munadziroh	PROPN
mkg-997	65	11	:	:	PUNCT
mkg-997	65	12	isolation	isolation	NOUN
mkg-997	65	13	and	and	CCONJ
mkg-997	65	14	identification	identification	NOUN
mkg-997	65	15	of	of	ADP
mkg-997	65	16	java	java	PROPN
mkg-997	65	17	race	race	PROPN
mkg-997	65	18	amniotic	amniotic	ADJ
mkg-997	65	19	membrane	membrane	NOUN
mkg-997	65	20	with	with	ADP
mkg-997	65	21	2	2	NUM
mkg-997	65	22	µl	µl	ADP
mkg-997	65	23	loading	loading	NOUN
mkg-997	65	24	dye	dye	NOUN
mkg-997	65	25	into	into	ADP
mkg-997	65	26	well	well	ADV
mkg-997	65	27	agarose	agarose	VERB
mkg-997	65	28	.	.	PUNCT
mkg-997	66	1	next	next	ADV
mkg-997	66	2	,	,	PUNCT
mkg-997	66	3	it	it	PRON
mkg-997	66	4	was	be	AUX
mkg-997	66	5	run	run	VERB
mkg-997	66	6	in	in	ADP
mkg-997	66	7	100	100	NUM
mkg-997	66	8	volt	volt	NOUN
mkg-997	66	9	for	for	ADP
mkg-997	66	10	about	about	ADV
mkg-997	66	11	30	30	NUM
mkg-997	66	12	minute	minute	NOUN
mkg-997	66	13	,	,	PUNCT
mkg-997	66	14	detected	detect	VERB
mkg-997	66	15	using	use	VERB
mkg-997	66	16	uvtransluminator	uvtransluminator	NOUN
mkg-997	66	17	finally	finally	ADV
mkg-997	66	18	documented	document	VERB
mkg-997	66	19	by	by	ADP
mkg-997	66	20	polaroid	polaroid	PROPN
mkg-997	66	21	camera	camera	NOUN
mkg-997	66	22	.	.	PUNCT
mkg-997	67	1	sequencing	sequence	VERB
mkg-997	67	2	process	process	NOUN
mkg-997	67	3	is	be	AUX
mkg-997	67	4	5	5	NUM
mkg-997	67	5	µl	µl	ADP
mkg-997	67	6	sodium	sodium	NOUN
mkg-997	67	7	acetate	acetate	NOUN
mkg-997	67	8	3	3	NUM
mkg-997	67	9	m	m	NOUN
mkg-997	67	10	was	be	AUX
mkg-997	67	11	added	add	VERB
mkg-997	67	12	into	into	ADP
mkg-997	67	13	sample	sample	NOUN
mkg-997	67	14	,	,	PUNCT
mkg-997	67	15	followed	follow	VERB
mkg-997	67	16	by	by	ADP
mkg-997	67	17	50	50	NUM
mkg-997	67	18	µl	µl	ADP
mkg-997	67	19	alcohol	alcohol	NOUN
mkg-997	67	20	100	100	NUM
mkg-997	67	21	%	%	NOUN
mkg-997	67	22	and	and	CCONJ
mkg-997	67	23	the	the	DET
mkg-997	67	24	eppendorf	eppendorf	NOUN
mkg-997	67	25	was	be	AUX
mkg-997	67	26	agitated	agitate	VERB
mkg-997	67	27	.	.	PUNCT
mkg-997	68	1	the	the	DET
mkg-997	68	2	mixture	mixture	NOUN
mkg-997	68	3	was	be	AUX
mkg-997	68	4	than	than	SCONJ
mkg-997	68	5	incubated	incubate	VERB
mkg-997	68	6	at	at	ADP
mkg-997	68	7	room	room	NOUN
mkg-997	68	8	temperature	temperature	NOUN
mkg-997	68	9	for	for	ADP
mkg-997	68	10	15	15	NUM
mkg-997	68	11	minute	minute	NOUN
mkg-997	68	12	and	and	CCONJ
mkg-997	68	13	continued	continue	VERB
mkg-997	68	14	by	by	ADP
mkg-997	68	15	centrifugation	centrifugation	NOUN
mkg-997	68	16	at	at	ADP
mkg-997	68	17	15.000	15.000	NUM
mkg-997	68	18	rpm	rpm	NOUN
mkg-997	68	19	at	at	ADP
mkg-997	68	20	40	40	NUM
mkg-997	68	21	c	c	NOUN
mkg-997	68	22	in	in	ADP
mkg-997	68	23	15	15	NUM
mkg-997	68	24	minute	minute	NOUN
mkg-997	68	25	.	.	PUNCT
mkg-997	69	1	supernatant	supernatant	NOUN
mkg-997	69	2	was	be	AUX
mkg-997	69	3	evacuated	evacuate	VERB
mkg-997	69	4	again	again	ADV
mkg-997	69	5	,	,	PUNCT
mkg-997	69	6	and	and	CCONJ
mkg-997	69	7	drying	dry	VERB
mkg-997	69	8	process	process	NOUN
mkg-997	69	9	in	in	ADP
mkg-997	69	10	vacuum	vacuum	PROPN
mkg-997	69	11	15	15	NUM
mkg-997	69	12	min	min	NOUN
mkg-997	69	13	.	.	PUNCT
mkg-997	70	1	the	the	DET
mkg-997	70	2	result	result	NOUN
mkg-997	70	3	was	be	AUX
mkg-997	70	4	ready	ready	ADJ
mkg-997	70	5	to	to	PART
mkg-997	70	6	kept	keep	VERB
mkg-997	70	7	or	or	CCONJ
mkg-997	70	8	directly	directly	ADV
mkg-997	70	9	sequenced.25	sequenced.25	VERB
mkg-997	70	10	µl	µl	ADP
mkg-997	70	11	template	template	NOUN
mkg-997	70	12	suppression	suppression	NOUN
mkg-997	70	13	reagent	reagent	NOUN
mkg-997	70	14	(	(	PUNCT
mkg-997	70	15	tsr	tsr	PROPN
mkg-997	70	16	)	)	PUNCT
mkg-997	70	17	was	be	AUX
mkg-997	70	18	added	add	VERB
mkg-997	70	19	into	into	ADP
mkg-997	70	20	pure	pure	ADJ
mkg-997	70	21	dna	dna	NOUN
mkg-997	70	22	and	and	CCONJ
mkg-997	70	23	dried	dry	VERB
mkg-997	70	24	at	at	ADP
mkg-997	70	25	950	950	NUM
mkg-997	70	26	c	c	NOUN
mkg-997	70	27	for	for	ADP
mkg-997	70	28	5	5	NUM
mkg-997	70	29	minute	minute	NOUN
mkg-997	70	30	,	,	PUNCT
mkg-997	70	31	then	then	ADV
mkg-997	70	32	,	,	PUNCT
mkg-997	70	33	put	put	VERB
mkg-997	70	34	into	into	ADP
mkg-997	70	35	the	the	DET
mkg-997	70	36	ice	ice	NOUN
mkg-997	70	37	for	for	ADP
mkg-997	70	38	10	10	NUM
mkg-997	70	39	minute	minute	NOUN
mkg-997	70	40	,	,	PUNCT
mkg-997	70	41	moved	move	VERB
mkg-997	70	42	into	into	ADP
mkg-997	70	43	0.5	0.5	NUM
mkg-997	70	44	ml	ml	NOUN
mkg-997	70	45	(	(	PUNCT
mkg-997	70	46	500µl	500µl	NOUN
mkg-997	70	47	)	)	PUNCT
mkg-997	70	48	tube	tube	NOUN
mkg-997	70	49	,	,	PUNCT
mkg-997	70	50	finally	finally	ADV
mkg-997	70	51	placed	place	VERB
mkg-997	70	52	into	into	ADP
mkg-997	70	53	sequencing	sequence	VERB
mkg-997	70	54	machine	machine	NOUN
mkg-997	70	55	for	for	ADP
mkg-997	70	56	one	one	NUM
mkg-997	70	57	hour	hour	NOUN
mkg-997	70	58	(	(	PUNCT
mkg-997	70	59	10	10	NUM
mkg-997	70	60	nucleotide	nucleotide	NOUN
mkg-997	70	61	for	for	ADP
mkg-997	70	62	1	1	NUM
mkg-997	70	63	minute	minute	NOUN
mkg-997	70	64	)	)	PUNCT
mkg-997	70	65	.	.	PUNCT
mkg-997	71	1	amplification	amplification	NOUN
mkg-997	71	2	,	,	PUNCT
mkg-997	71	3	purification	purification	NOUN
mkg-997	71	4	and	and	CCONJ
mkg-997	71	5	sequencing	sequence	VERB
mkg-997	71	6	gen	gen	NOUN
mkg-997	71	7	encoding	encoding	NOUN
mkg-997	71	8	slpi	slpi	NOUN
mkg-997	71	9	amniotic	amniotic	ADJ
mkg-997	71	10	membrane	membrane	NOUN
mkg-997	71	11	:	:	PUNCT
mkg-997	71	12	gen	gen	PROPN
mkg-997	71	13	encoding	encoding	NOUN
mkg-997	71	14	slpi	slpi	NOUN
mkg-997	71	15	could	could	AUX
mkg-997	71	16	be	be	AUX
mkg-997	71	17	amplified	amplify	VERB
mkg-997	71	18	after	after	SCONJ
mkg-997	71	19	rna	rna	NOUN
mkg-997	71	20	isolation	isolation	NOUN
mkg-997	71	21	was	be	AUX
mkg-997	71	22	previously	previously	ADV
mkg-997	71	23	done	do	VERB
mkg-997	71	24	from	from	ADP
mkg-997	71	25	amniotic	amniotic	ADJ
mkg-997	71	26	membrane	membrane	NOUN
mkg-997	71	27	using	use	VERB
mkg-997	71	28	rneasy	rneasy	PROPN
mkg-997	71	29	mini	mini	PROPN
mkg-997	71	30	kit	kit	PROPN
mkg-997	71	31	.	.	PUNCT
mkg-997	72	1	50µl	50µl	ADJ
mkg-997	72	2	sample	sample	NOUN
mkg-997	72	3	was	be	AUX
mkg-997	72	4	put	put	VERB
mkg-997	72	5	into	into	ADP
mkg-997	72	6	eppendorf	eppendorf	ADJ
mkg-997	72	7	tube	tube	NOUN
mkg-997	72	8	(	(	PUNCT
mkg-997	72	9	1.5	1.5	NUM
mkg-997	72	10	ml	ml	NOUN
mkg-997	72	11	)	)	PUNCT
mkg-997	72	12	,	,	PUNCT
mkg-997	72	13	added	add	VERB
mkg-997	72	14	by	by	ADP
mkg-997	72	15	550	550	NUM
mkg-997	72	16	µl	µl	ADP
mkg-997	72	17	b	b	NOUN
mkg-997	72	18	–	–	PUNCT
mkg-997	72	19	me	i	PRON
mkg-997	72	20	/	/	SYM
mkg-997	72	21	rlt	rlt	NOUN
mkg-997	72	22	and	and	CCONJ
mkg-997	72	23	600	600	NUM
mkg-997	72	24	µl	µl	ADP
mkg-997	72	25	ethanol	ethanol	NOUN
mkg-997	72	26	70	70	NUM
mkg-997	72	27	%	%	NOUN
mkg-997	72	28	into	into	ADP
mkg-997	72	29	the	the	DET
mkg-997	72	30	sample	sample	NOUN
mkg-997	72	31	,	,	PUNCT
mkg-997	72	32	then	then	ADV
mkg-997	72	33	,	,	PUNCT
mkg-997	72	34	mixed	mix	VERB
mkg-997	72	35	by	by	ADP
mkg-997	72	36	agitating	agitate	VERB
mkg-997	72	37	the	the	DET
mkg-997	72	38	tube	tube	NOUN
mkg-997	72	39	.	.	PUNCT
mkg-997	73	1	600µl	600µl	ADJ
mkg-997	73	2	solution	solution	NOUN
mkg-997	73	3	was	be	AUX
mkg-997	73	4	moved	move	VERB
mkg-997	73	5	into	into	ADP
mkg-997	73	6	rneasy	rneasy	NOUN
mkg-997	73	7	spin	spin	NOUN
mkg-997	73	8	column	column	NOUN
mkg-997	73	9	and	and	CCONJ
mkg-997	73	10	kept	keep	VERB
mkg-997	73	11	in	in	ADP
mkg-997	73	12	collective	collective	ADJ
mkg-997	73	13	tube	tube	NOUN
mkg-997	73	14	2ml	2ml	NOUN
mkg-997	73	15	,	,	PUNCT
mkg-997	73	16	followed	follow	VERB
mkg-997	73	17	by	by	ADP
mkg-997	73	18	centrifugation	centrifugation	NOUN
mkg-997	73	19	at	at	ADP
mkg-997	73	20	12.000	12.000	NUM
mkg-997	73	21	rpm	rpm	NOUN
mkg-997	73	22	for15sec	for15sec	NOUN
mkg-997	73	23	.	.	PUNCT
mkg-997	74	1	the	the	DET
mkg-997	74	2	lower	low	ADJ
mkg-997	74	3	part	part	NOUN
mkg-997	74	4	of	of	ADP
mkg-997	74	5	the	the	DET
mkg-997	74	6	solution	solution	NOUN
mkg-997	74	7	was	be	AUX
mkg-997	74	8	removed	remove	VERB
mkg-997	74	9	and	and	CCONJ
mkg-997	74	10	the	the	DET
mkg-997	74	11	remain	remain	NOUN
mkg-997	74	12	was	be	AUX
mkg-997	74	13	repeated	repeat	VERB
mkg-997	74	14	.	.	PUNCT
mkg-997	75	1	700	700	NUM
mkg-997	75	2	µl	µl	ADP
mkg-997	75	3	rw1	rw1	NOUN
mkg-997	75	4	solution	solution	NOUN
mkg-997	75	5	was	be	AUX
mkg-997	75	6	added	add	VERB
mkg-997	75	7	into	into	ADP
mkg-997	75	8	rneasy	rneasy	NOUN
mkg-997	75	9	column	column	NOUN
mkg-997	75	10	followed	follow	VERB
mkg-997	75	11	by	by	ADP
mkg-997	75	12	centrifugation	centrifugation	NOUN
mkg-997	75	13	at	at	ADP
mkg-997	75	14	12.000	12.000	NUM
mkg-997	75	15	rpm	rpm	NOUN
mkg-997	75	16	for	for	ADP
mkg-997	75	17	15	15	NUM
mkg-997	75	18	sec	sec	PROPN
mkg-997	75	19	.	.	PROPN
mkg-997	76	1	and	and	CCONJ
mkg-997	76	2	the	the	DET
mkg-997	76	3	lower	low	ADJ
mkg-997	76	4	part	part	NOUN
mkg-997	76	5	of	of	ADP
mkg-997	76	6	the	the	DET
mkg-997	76	7	solution	solution	NOUN
mkg-997	76	8	was	be	AUX
mkg-997	76	9	removed	remove	VERB
mkg-997	76	10	.	.	PUNCT
mkg-997	77	1	rneasy	rneasy	NOUN
mkg-997	77	2	column	column	PROPN
mkg-997	77	3	was	be	AUX
mkg-997	77	4	moved	move	VERB
mkg-997	77	5	to	to	ADP
mkg-997	77	6	a	a	DET
mkg-997	77	7	new	new	ADJ
mkg-997	77	8	tube	tube	NOUN
mkg-997	77	9	(	(	PUNCT
mkg-997	77	10	2	2	NUM
mkg-997	77	11	ml	ml	NOUN
mkg-997	77	12	)	)	PUNCT
mkg-997	77	13	,	,	PUNCT
mkg-997	77	14	added	add	VERB
mkg-997	77	15	by	by	ADP
mkg-997	77	16	500µl	500µl	ADJ
mkg-997	77	17	rpe	rpe	PROPN
mkg-997	77	18	buffer	buffer	NOUN
mkg-997	77	19	and	and	CCONJ
mkg-997	77	20	centrifuged	centrifuge	VERB
mkg-997	77	21	at	at	ADP
mkg-997	77	22	12.000	12.000	NUM
mkg-997	77	23	rpm	rpm	NOUN
mkg-997	77	24	for	for	ADP
mkg-997	77	25	15	15	NUM
mkg-997	77	26	sec	sec	PROPN
mkg-997	77	27	.	.	PUNCT
mkg-997	78	1	the	the	DET
mkg-997	78	2	lower	low	ADJ
mkg-997	78	3	part	part	NOUN
mkg-997	78	4	of	of	ADP
mkg-997	78	5	the	the	DET
mkg-997	78	6	solution	solution	NOUN
mkg-997	78	7	was	be	AUX
mkg-997	78	8	removed	remove	VERB
mkg-997	78	9	and	and	CCONJ
mkg-997	78	10	repeated	repeat	VERB
mkg-997	78	11	.	.	PUNCT
mkg-997	79	1	rneasy	rneasy	NOUN
mkg-997	79	2	column	column	PROPN
mkg-997	79	3	was	be	AUX
mkg-997	79	4	moved	move	VERB
mkg-997	79	5	into	into	ADP
mkg-997	79	6	another	another	DET
mkg-997	79	7	tube	tube	NOUN
mkg-997	79	8	then	then	ADV
mkg-997	79	9	centrifuged	centrifuge	VERB
mkg-997	79	10	at	at	ADP
mkg-997	79	11	13.000	13.000	NUM
mkg-997	79	12	rpm	rpm	NOUN
mkg-997	79	13	for	for	ADP
mkg-997	79	14	2	2	NUM
mkg-997	79	15	minute	minute	NOUN
mkg-997	79	16	(	(	PUNCT
mkg-997	79	17	to	to	PART
mkg-997	79	18	release	release	VERB
mkg-997	79	19	the	the	DET
mkg-997	79	20	ethanol	ethanol	NOUN
mkg-997	79	21	)	)	PUNCT
mkg-997	79	22	.	.	PUNCT
mkg-997	80	1	rneasy	rneasy	PROPN
mkg-997	80	2	column	column	PROPN
mkg-997	80	3	was	be	AUX
mkg-997	80	4	moved	move	VERB
mkg-997	80	5	again	again	ADV
mkg-997	80	6	into	into	ADP
mkg-997	80	7	15ml	15ml	ADJ
mkg-997	80	8	tube	tube	NOUN
mkg-997	80	9	,	,	PUNCT
mkg-997	80	10	next	next	ADV
mkg-997	80	11	,	,	PUNCT
mkg-997	80	12	added	add	VERB
mkg-997	80	13	by	by	ADP
mkg-997	80	14	30	30	NUM
mkg-997	80	15	µl	µl	NOUN
mkg-997	80	16	rneasy	rneasy	NOUN
mkg-997	80	17	free	free	ADJ
mkg-997	80	18	water	water	NOUN
mkg-997	80	19	in	in	ADP
mkg-997	80	20	the	the	DET
mkg-997	80	21	middle	middle	ADJ
mkg-997	80	22	part	part	NOUN
mkg-997	80	23	of	of	ADP
mkg-997	80	24	the	the	DET
mkg-997	80	25	column	column	NOUN
mkg-997	80	26	,	,	PUNCT
mkg-997	80	27	centrifugation	centrifugation	NOUN
mkg-997	80	28	was	be	AUX
mkg-997	80	29	done	do	VERB
mkg-997	80	30	at	at	ADP
mkg-997	80	31	12.000	12.000	NUM
mkg-997	80	32	rpm	rpm	NOUN
mkg-997	80	33	for	for	ADP
mkg-997	80	34	2	2	NUM
mkg-997	80	35	minutes	minute	NOUN
mkg-997	80	36	.	.	PUNCT
mkg-997	81	1	reverse	reverse	VERB
mkg-997	81	2	transcriptase	transcriptase	NOUN
mkg-997	81	3	to	to	PART
mkg-997	81	4	obtain	obtain	VERB
mkg-997	81	5	cdna	cdna	PROPN
mkg-997	81	6	product	product	NOUN
mkg-997	81	7	was	be	AUX
mkg-997	81	8	done	do	VERB
mkg-997	81	9	on	on	ADP
mkg-997	81	10	the	the	DET
mkg-997	81	11	lower	low	ADJ
mkg-997	81	12	part	part	NOUN
mkg-997	81	13	of	of	ADP
mkg-997	81	14	the	the	DET
mkg-997	81	15	solution	solution	NOUN
mkg-997	81	16	containing	contain	VERB
mkg-997	81	17	collective	collective	ADJ
mkg-997	81	18	rna	rna	PROPN
mkg-997	81	19	.	.	PUNCT
mkg-997	82	1	this	this	DET
mkg-997	82	2	present	present	ADJ
mkg-997	82	3	study	study	NOUN
mkg-997	82	4	applied	apply	VERB
mkg-997	82	5	two	two	NUM
mkg-997	82	6	step	step	NOUN
mkg-997	82	7	pcr	pcr	NOUN
mkg-997	82	8	method	method	NOUN
mkg-997	82	9	and	and	CCONJ
mkg-997	82	10	primer	primer	NOUN
mkg-997	82	11	was	be	AUX
mkg-997	82	12	designed	design	VERB
mkg-997	82	13	to	to	PART
mkg-997	82	14	fulfill	fulfill	VERB
mkg-997	82	15	the	the	DET
mkg-997	82	16	criteria	criterion	NOUN
mkg-997	82	17	of	of	ADP
mkg-997	82	18	gen	gen	PROPN
mkg-997	82	19	insertion	insertion	NOUN
mkg-997	82	20	in	in	ADP
mkg-997	82	21	pet	pet	ADJ
mkg-997	82	22	101	101	NUM
mkg-997	82	23	/	/	SYM
mkg-997	82	24	d	d	NOUN
mkg-997	82	25	-	-	NOUN
mkg-997	82	26	topo	topo	NOUN
mkg-997	82	27	by	by	ADP
mkg-997	82	28	adding	add	VERB
mkg-997	82	29	cacc	cacc	ADV
mkg-997	82	30	prior	prior	ADV
mkg-997	82	31	to	to	ADP
mkg-997	82	32	atg	atg	PROPN
mkg-997	82	33	(	(	PUNCT
mkg-997	82	34	at	at	ADP
mkg-997	82	35	initial	initial	ADJ
mkg-997	82	36	gen	gen	PROPN
mkg-997	82	37	)	)	PUNCT
mkg-997	82	38	i.e.	i.e.	X
mkg-997	82	39	primer	primer	NOUN
mkg-997	82	40	(	(	PUNCT
mkg-997	82	41	sense	sense	NOUN
mkg-997	82	42	):	):	PUNCT
mkg-997	82	43	5	5	NUM
mkg-997	82	44	’	'	PUNCT
mkg-997	82	45	cac	cac	PROPN
mkg-997	82	46	cat	cat	PROPN
mkg-997	82	47	gaa	gaa	PROPN
mkg-997	82	48	gtc	gtc	PROPN
mkg-997	82	49	cag	cag	PROPN
mkg-997	82	50	cgg	cgg	PROPN
mkg-997	82	51	cct	cct	PROPN
mkg-997	82	52	ctt	ctt	PROPN
mkg-997	82	53	cc	cc	PROPN
mkg-997	82	54	3	3	NUM
mkg-997	82	55	’	'	PUNCT
mkg-997	82	56	and	and	CCONJ
mkg-997	82	57	primer	primer	NOUN
mkg-997	82	58	(	(	PUNCT
mkg-997	82	59	anti	anti	X
mkg-997	82	60	sense)5	sense)5	NOUN
mkg-997	82	61	’	'	PUNCT
mkg-997	82	62	agc	agc	PROPN
mkg-997	82	63	ttt	ttt	PROPN
mkg-997	82	64	cac	cac	PROPN
mkg-997	82	65	agg	agg	PROPN
mkg-997	82	66	gga	gga	PROPN
mkg-997	82	67	aac	aac	PROPN
mkg-997	82	68	gca	gca	PROPN
mkg-997	82	69	gga	gga	PROPN
mkg-997	82	70	t	t	PROPN
mkg-997	82	71	3	3	NUM
mkg-997	82	72	’	'	PUNCT
mkg-997	82	73	.	.	PUNCT
mkg-997	83	1	amplification	amplification	NOUN
mkg-997	83	2	was	be	AUX
mkg-997	83	3	done	do	VERB
mkg-997	83	4	using	use	VERB
mkg-997	83	5	reagent	reagent	ADJ
mkg-997	83	6	mixture	mixture	NOUN
mkg-997	83	7	consisting	consist	VERB
mkg-997	83	8	of	of	ADP
mkg-997	83	9	:	:	PUNCT
mkg-997	83	10	70µl	70µl	ADJ
mkg-997	83	11	distillated	distillate	VERB
mkg-997	83	12	water	water	NOUN
mkg-997	83	13	mixed	mix	VERB
mkg-997	83	14	with	with	ADP
mkg-997	83	15	9	9	NUM
mkg-997	83	16	µl	µl	ADP
mkg-997	83	17	reaction	reaction	NOUN
mkg-997	83	18	buffer	buffer	NOUN
mkg-997	83	19	tth	tth	PROPN
mkg-997	83	20	,	,	PUNCT
mkg-997	83	21	8	8	NUM
mkg-997	83	22	µl	µl	ADP
mkg-997	83	23	dntp	dntp	NOUN
mkg-997	83	24	mix	mix	NOUN
mkg-997	83	25	,	,	PUNCT
mkg-997	83	26	1µl	1µl	ADJ
mkg-997	83	27	primer	primer	NOUN
mkg-997	83	28	forward	forward	ADV
mkg-997	83	29	and	and	CCONJ
mkg-997	83	30	1µl	1µl	ADJ
mkg-997	83	31	primer	primer	NOUN
mkg-997	83	32	reverse	reverse	NOUN
mkg-997	83	33	,	,	PUNCT
mkg-997	83	34	10µl	10µl	ADJ
mkg-997	83	35	cdna	cdna	PROPN
mkg-997	83	36	(	(	PUNCT
mkg-997	83	37	total	total	ADJ
mkg-997	83	38	99	99	NUM
mkg-997	83	39	µl	µl	NOUN
mkg-997	83	40	)	)	PUNCT
mkg-997	83	41	and	and	CCONJ
mkg-997	83	42	added	add	VERB
mkg-997	83	43	by	by	ADP
mkg-997	83	44	mineral	mineral	NOUN
mkg-997	83	45	oil	oil	NOUN
mkg-997	83	46	.	.	PUNCT
mkg-997	84	1	the	the	DET
mkg-997	84	2	process	process	NOUN
mkg-997	84	3	was	be	AUX
mkg-997	84	4	done	do	VERB
mkg-997	84	5	according	accord	VERB
mkg-997	84	6	to	to	ADP
mkg-997	84	7	the	the	DET
mkg-997	84	8	following	following	ADJ
mkg-997	84	9	stages	stage	NOUN
mkg-997	84	10	:	:	PUNCT
mkg-997	84	11	predenaturation	predenaturation	NOUN
mkg-997	84	12	at	at	ADP
mkg-997	84	13	at	at	ADP
mkg-997	84	14	72	72	NUM
mkg-997	84	15	°	°	ADP
mkg-997	84	16	c	c	NOUN
mkg-997	84	17	for	for	ADP
mkg-997	84	18	sec	sec	PROPN
mkg-997	84	19	.	.	PROPN
mkg-997	85	1	and	and	CCONJ
mkg-997	85	2	94	94	NUM
mkg-997	85	3	°	°	NOUN
mkg-997	85	4	c	c	NOUN
mkg-997	85	5	for	for	ADP
mkg-997	85	6	5	5	NUM
mkg-997	85	7	min	min	NOUN
mkg-997	85	8	.	.	PUNCT
mkg-997	86	1	the	the	DET
mkg-997	86	2	mixture	mixture	NOUN
mkg-997	86	3	was	be	AUX
mkg-997	86	4	then	then	ADV
mkg-997	86	5	added	add	VERB
mkg-997	86	6	by	by	ADP
mkg-997	86	7	1µl	1µl	PROPN
mkg-997	86	8	tth	tth	PROPN
mkg-997	86	9	dna	dna	PROPN
mkg-997	86	10	polymerase	polymerase	NOUN
mkg-997	86	11	enzyme	enzyme	NOUN
mkg-997	86	12	,	,	PUNCT
mkg-997	86	13	put	put	VERB
mkg-997	86	14	into	into	ADP
mkg-997	86	15	pcr	pcr	ADJ
mkg-997	86	16	machine	machine	NOUN
mkg-997	86	17	with	with	ADP
mkg-997	86	18	denaturation	denaturation	NOUN
mkg-997	86	19	process	process	NOUN
mkg-997	86	20	at	at	ADP
mkg-997	86	21	94	94	NUM
mkg-997	86	22	°	°	NOUN
mkg-997	86	23	c	c	NOUN
mkg-997	86	24	for	for	ADP
mkg-997	86	25	40	40	NUM
mkg-997	86	26	sec	sec	PROPN
mkg-997	86	27	.	.	PROPN
mkg-997	86	28	followed	follow	VERB
mkg-997	86	29	by	by	ADP
mkg-997	86	30	annealing	anneal	VERB
mkg-997	86	31	at	at	ADP
mkg-997	86	32	52	52	NUM
mkg-997	86	33	°	°	NOUN
mkg-997	86	34	c	c	NOUN
mkg-997	86	35	for	for	ADP
mkg-997	86	36	40	40	NUM
mkg-997	86	37	sec	sec	PROPN
mkg-997	86	38	.	.	PROPN
mkg-997	87	1	and	and	CCONJ
mkg-997	87	2	extension	extension	NOUN
mkg-997	87	3	at	at	ADP
mkg-997	87	4	72	72	NUM
mkg-997	87	5	°	°	ADP
mkg-997	87	6	c	c	NOUN
mkg-997	87	7	for	for	ADP
mkg-997	87	8	40	40	NUM
mkg-997	87	9	sec	sec	PROPN
mkg-997	87	10	,	,	PUNCT
mkg-997	87	11	next	next	ADJ
mkg-997	87	12	final	final	ADJ
mkg-997	87	13	step	step	NOUN
mkg-997	87	14	extra	extra	ADJ
mkg-997	87	15	extension	extension	NOUN
mkg-997	87	16	at	at	ADP
mkg-997	87	17	72	72	NUM
mkg-997	87	18	°	°	ADP
mkg-997	87	19	c	c	NOUN
mkg-997	87	20	for	for	ADP
mkg-997	87	21	10	10	NUM
mkg-997	87	22	sec	sec	PROPN
mkg-997	87	23	.	.	PUNCT
mkg-997	88	1	pcr	pcr	PROPN
mkg-997	88	2	was	be	AUX
mkg-997	88	3	done	do	VERB
mkg-997	88	4	in	in	ADP
mkg-997	88	5	35	35	NUM
mkg-997	88	6	cycles	cycle	NOUN
mkg-997	88	7	.	.	PUNCT
mkg-997	89	1	the	the	DET
mkg-997	89	2	result	result	NOUN
mkg-997	89	3	of	of	ADP
mkg-997	89	4	pcr	pcr	NOUN
mkg-997	89	5	could	could	AUX
mkg-997	89	6	be	be	AUX
mkg-997	89	7	seen	see	VERB
mkg-997	89	8	by	by	ADP
mkg-997	89	9	electrophoresis	electrophoresis	NOUN
mkg-997	89	10	in	in	ADP
mkg-997	89	11	2	2	NUM
mkg-997	89	12	%	%	NOUN
mkg-997	89	13	gel	gel	NOUN
mkg-997	89	14	agarose	agarose	NOUN
mkg-997	89	15	containing	contain	VERB
mkg-997	89	16	ethidium	ethidium	ADJ
mkg-997	89	17	bromide	bromide	NOUN
mkg-997	89	18	,	,	PUNCT
mkg-997	89	19	5µl	5µl	ADJ
mkg-997	89	20	cdna	cdna	PROPN
mkg-997	89	21	added	add	VERB
mkg-997	89	22	by	by	ADP
mkg-997	89	23	2µl	2µl	ADJ
mkg-997	89	24	loading	loading	NOUN
mkg-997	89	25	dye	dye	NOUN
mkg-997	89	26	put	put	VERB
mkg-997	89	27	into	into	ADP
mkg-997	89	28	well	well	ADV
mkg-997	89	29	agarose	agarose	NOUN
mkg-997	89	30	and	and	CCONJ
mkg-997	89	31	operated	operate	VERB
mkg-997	89	32	in	in	ADP
mkg-997	89	33	100	100	NUM
mkg-997	89	34	volt	volt	NOUN
mkg-997	89	35	approximately	approximately	ADV
mkg-997	89	36	30	30	NUM
mkg-997	89	37	min	min	NOUN
mkg-997	89	38	,	,	PUNCT
mkg-997	89	39	continued	continue	VERB
mkg-997	89	40	by	by	ADP
mkg-997	89	41	uv	uv	NOUN
mkg-997	89	42	-	-	PUNCT
mkg-997	89	43	transluminator	transluminator	NOUN
mkg-997	89	44	detection	detection	NOUN
mkg-997	89	45	,	,	PUNCT
mkg-997	89	46	and	and	CCONJ
mkg-997	89	47	documentation	documentation	NOUN
mkg-997	89	48	was	be	AUX
mkg-997	89	49	taken	take	VERB
mkg-997	89	50	using	use	VERB
mkg-997	89	51	polaroid	polaroid	PROPN
mkg-997	89	52	camera	camera	NOUN
mkg-997	89	53	.	.	PUNCT
mkg-997	90	1	preparation	preparation	NOUN
mkg-997	90	2	for	for	ADP
mkg-997	90	3	sequencing	sequence	VERB
mkg-997	90	4	and	and	CCONJ
mkg-997	90	5	cloning	cloning	NOUN
mkg-997	90	6	,	,	PUNCT
mkg-997	90	7	the	the	DET
mkg-997	90	8	pcr	pcr	ADJ
mkg-997	90	9	product	product	NOUN
mkg-997	90	10	should	should	AUX
mkg-997	90	11	be	be	AUX
mkg-997	90	12	previously	previously	ADV
mkg-997	90	13	purified	purify	VERB
mkg-997	90	14	.	.	PUNCT
mkg-997	91	1	the	the	DET
mkg-997	91	2	product	product	NOUN
mkg-997	91	3	was	be	AUX
mkg-997	91	4	run	run	VERB
mkg-997	91	5	in	in	ADP
mkg-997	91	6	1.5	1.5	NUM
mkg-997	91	7	%	%	NOUN
mkg-997	91	8	low	low	ADJ
mkg-997	91	9	melting	melting	NOUN
mkg-997	91	10	agar	agar	NOUN
mkg-997	91	11	,	,	PUNCT
mkg-997	91	12	dna	dna	PROPN
mkg-997	91	13	fragment	fragment	NOUN
mkg-997	91	14	was	be	AUX
mkg-997	91	15	cut	cut	VERB
mkg-997	91	16	from	from	ADP
mkg-997	91	17	gel	gel	NOUN
mkg-997	91	18	agarose	agarose	NOUN
mkg-997	91	19	with	with	ADP
mkg-997	91	20	scalpel	scalpel	NOUN
mkg-997	91	21	.	.	PUNCT
mkg-997	92	1	under	under	ADP
mkg-997	92	2	uv	uv	NOUN
mkg-997	92	3	light	light	NOUN
mkg-997	92	4	,	,	PUNCT
mkg-997	92	5	gel	gel	NOUN
mkg-997	92	6	cutting	cutting	NOUN
mkg-997	92	7	containing	contain	VERB
mkg-997	92	8	dna	dna	PROPN
mkg-997	92	9	fragment	fragment	NOUN
mkg-997	92	10	added	add	VERB
mkg-997	92	11	by	by	ADP
mkg-997	92	12	3	3	NUM
mkg-997	92	13	volume	volume	NOUN
mkg-997	92	14	of	of	ADP
mkg-997	92	15	qg	qg	PROPN
mkg-997	92	16	buffer	buffer	NOUN
mkg-997	92	17	in	in	ADP
mkg-997	92	18	1	1	NUM
mkg-997	92	19	volume	volume	NOUN
mkg-997	92	20	gel	gel	NOUN
mkg-997	92	21	(	(	PUNCT
mkg-997	92	22	100	100	NUM
mkg-997	92	23	mg	mg	NUM
mkg-997	92	24	±	±	NUM
mkg-997	92	25	100	100	NUM
mkg-997	92	26	µl	µl	NOUN
mkg-997	92	27	)	)	PUNCT
mkg-997	92	28	the	the	DET
mkg-997	92	29	ext	ext	NOUN
mkg-997	92	30	step	step	NOUN
mkg-997	92	31	,	,	PUNCT
mkg-997	92	32	incubation	incubation	NOUN
mkg-997	92	33	was	be	AUX
mkg-997	92	34	performed	perform	VERB
mkg-997	92	35	at	at	ADP
mkg-997	92	36	50	50	NUM
mkg-997	92	37	°	°	NOUN
mkg-997	92	38	c	c	NOUN
mkg-997	92	39	for	for	ADP
mkg-997	92	40	10	10	NUM
mkg-997	92	41	min	min	NOUN
mkg-997	92	42	until	until	SCONJ
mkg-997	92	43	all	all	DET
mkg-997	92	44	gel	gel	NOUN
mkg-997	92	45	was	be	AUX
mkg-997	92	46	dissolved	dissolve	VERB
mkg-997	92	47	and	and	CCONJ
mkg-997	92	48	a	a	DET
mkg-997	92	49	mixture	mixture	NOUN
mkg-997	92	50	of	of	ADP
mkg-997	92	51	gel	gel	NOUN
mkg-997	92	52	and	and	CCONJ
mkg-997	92	53	vertex	vertex	NOUN
mkg-997	92	54	was	be	AUX
mkg-997	92	55	done	do	VERB
mkg-997	92	56	in	in	ADP
mkg-997	92	57	tube	tube	NOUN
mkg-997	92	58	every	every	DET
mkg-997	92	59	2–3	2–3	NUM
mkg-997	92	60	minute	minute	NOUN
mkg-997	92	61	during	during	ADP
mkg-997	92	62	the	the	DET
mkg-997	92	63	incubation	incubation	NOUN
mkg-997	92	64	.	.	PUNCT
mkg-997	93	1	after	after	SCONJ
mkg-997	93	2	all	all	DET
mkg-997	93	3	gel	gel	NOUN
mkg-997	93	4	was	be	AUX
mkg-997	93	5	dissolved	dissolve	VERB
mkg-997	93	6	,	,	PUNCT
mkg-997	93	7	the	the	DET
mkg-997	93	8	color	color	NOUN
mkg-997	93	9	of	of	ADP
mkg-997	93	10	the	the	DET
mkg-997	93	11	solution	solution	NOUN
mkg-997	93	12	would	would	AUX
mkg-997	93	13	be	be	AUX
mkg-997	93	14	yellow	yellow	ADJ
mkg-997	93	15	similar	similar	ADJ
mkg-997	93	16	to	to	ADP
mkg-997	93	17	qg	qg	PROPN
mkg-997	93	18	buffer	buffer	NOUN
mkg-997	93	19	prior	prior	ADV
mkg-997	93	20	to	to	ADP
mkg-997	93	21	dissolving	dissolve	VERB
mkg-997	93	22	gel	gel	NOUN
mkg-997	93	23	.	.	PUNCT
mkg-997	94	1	1	1	NUM
mkg-997	94	2	volume	volume	NOUN
mkg-997	94	3	of	of	ADP
mkg-997	94	4	isopropanol	isopropanol	NOUN
mkg-997	94	5	was	be	AUX
mkg-997	94	6	mixed	mix	VERB
mkg-997	94	7	with	with	ADP
mkg-997	94	8	the	the	DET
mkg-997	94	9	sample	sample	NOUN
mkg-997	94	10	,	,	PUNCT
mkg-997	94	11	then	then	ADV
mkg-997	94	12	,	,	PUNCT
mkg-997	94	13	qia	qia	PROPN
mkg-997	94	14	quick	quick	ADJ
mkg-997	94	15	spin	spin	NOUN
mkg-997	94	16	was	be	AUX
mkg-997	94	17	placed	place	VERB
mkg-997	94	18	column	column	NOUN
mkg-997	94	19	in	in	ADP
mkg-997	94	20	2	2	NUM
mkg-997	94	21	ml	ml	ADP
mkg-997	94	22	collective	collective	ADJ
mkg-997	94	23	tube	tube	NOUN
mkg-997	94	24	and	and	CCONJ
mkg-997	94	25	sample	sample	NOUN
mkg-997	94	26	was	be	AUX
mkg-997	94	27	added	add	VERB
mkg-997	94	28	in	in	ADP
mkg-997	94	29	column	column	NOUN
mkg-997	94	30	,	,	PUNCT
mkg-997	94	31	then	then	ADV
mkg-997	94	32	it	it	PRON
mkg-997	94	33	was	be	AUX
mkg-997	94	34	centrifuged	centrifuge	VERB
mkg-997	94	35	at	at	ADP
mkg-997	94	36	13.000	13.000	NUM
mkg-997	94	37	rpm	rpm	NOUN
mkg-997	94	38	for	for	ADP
mkg-997	94	39	1	1	NUM
mkg-997	94	40	mm	mm	NOUN
mkg-997	94	41	.	.	PUNCT
mkg-997	95	1	the	the	DET
mkg-997	95	2	lower	low	ADJ
mkg-997	95	3	part	part	NOUN
mkg-997	95	4	of	of	ADP
mkg-997	95	5	the	the	DET
mkg-997	95	6	solution	solution	NOUN
mkg-997	95	7	was	be	AUX
mkg-997	95	8	removed	remove	VERB
mkg-997	95	9	,	,	PUNCT
mkg-997	95	10	then	then	ADV
mkg-997	95	11	,	,	PUNCT
mkg-997	95	12	column	column	NOUN
mkg-997	95	13	was	be	AUX
mkg-997	95	14	put	put	VERB
mkg-997	95	15	back	back	ADV
mkg-997	95	16	into	into	ADP
mkg-997	95	17	tube	tube	NOUN
mkg-997	95	18	.	.	PUNCT
mkg-997	96	1	750µl	750µl	NOUN
mkg-997	96	2	pe	pe	PROPN
mkg-997	96	3	buffer	buffer	NOUN
mkg-997	96	4	was	be	AUX
mkg-997	96	5	added	add	VERB
mkg-997	96	6	in	in	ADP
mkg-997	96	7	column	column	NOUN
mkg-997	96	8	,	,	PUNCT
mkg-997	96	9	followed	follow	VERB
mkg-997	96	10	by	by	ADP
mkg-997	96	11	centrifugation	centrifugation	NOUN
mkg-997	96	12	at	at	ADP
mkg-997	96	13	13.000	13.000	NUM
mkg-997	96	14	rpm	rpm	NOUN
mkg-997	96	15	for	for	ADP
mkg-997	96	16	1	1	NUM
mkg-997	96	17	min	min	NOUN
mkg-997	96	18	.	.	PUNCT
mkg-997	97	1	the	the	DET
mkg-997	97	2	solution	solution	NOUN
mkg-997	97	3	in	in	ADP
mkg-997	97	4	the	the	DET
mkg-997	97	5	lower	low	ADJ
mkg-997	97	6	part	part	NOUN
mkg-997	97	7	of	of	ADP
mkg-997	97	8	centrifugation	centrifugation	NOUN
mkg-997	97	9	was	be	AUX
mkg-997	97	10	thrown	throw	VERB
mkg-997	97	11	out	out	ADP
mkg-997	97	12	and	and	CCONJ
mkg-997	97	13	centrifugation	centrifugation	NOUN
mkg-997	97	14	was	be	AUX
mkg-997	97	15	performed	perform	VERB
mkg-997	97	16	again	again	ADV
mkg-997	97	17	at	at	ADP
mkg-997	97	18	13.000	13.000	NUM
mkg-997	97	19	rpm	rpm	NOUN
mkg-997	97	20	for	for	ADP
mkg-997	97	21	1	1	NUM
mkg-997	97	22	min	min	PROPN
mkg-997	97	23	.	.	PROPN
mkg-997	97	24	column	column	NOUN
mkg-997	97	25	was	be	AUX
mkg-997	97	26	placed	place	VERB
mkg-997	97	27	in	in	ADP
mkg-997	97	28	1	1	NUM
mkg-997	97	29	ml	ml	NOUN
mkg-997	97	30	tube	tube	NOUN
mkg-997	97	31	of	of	ADP
mkg-997	97	32	new	new	PROPN
mkg-997	97	33	micro	micro	NOUN
mkg-997	97	34	centrifuge	centrifuge	NOUN
mkg-997	97	35	.	.	PUNCT
mkg-997	98	1	next	next	ADV
mkg-997	98	2	,	,	PUNCT
mkg-997	98	3	it	it	PRON
mkg-997	98	4	was	be	AUX
mkg-997	98	5	added	add	VERB
mkg-997	98	6	by	by	ADP
mkg-997	98	7	50	50	NUM
mkg-997	98	8	µl	µl	ADP
mkg-997	98	9	eb	eb	PROPN
mkg-997	98	10	buffer	buffer	NOUN
mkg-997	98	11	(	(	PUNCT
mkg-997	98	12	10	10	NUM
mkg-997	98	13	mm	mm	NOUN
mkg-997	98	14	tris	tris	NOUN
mkg-997	98	15	-	-	PUNCT
mkg-997	98	16	cl	cl	NOUN
mkg-997	98	17	,	,	PUNCT
mkg-997	98	18	ph	ph	NOUN
mkg-997	98	19	8.5	8.5	NUM
mkg-997	98	20	)	)	PUNCT
mkg-997	98	21	or	or	CCONJ
mkg-997	98	22	h2o	h2o	PROPN
mkg-997	98	23	,	,	PUNCT
mkg-997	98	24	centrifugation	centrifugation	NOUN
mkg-997	98	25	was	be	AUX
mkg-997	98	26	done	do	VERB
mkg-997	98	27	at	at	ADP
mkg-997	98	28	13.000	13.000	NUM
mkg-997	98	29	rpm	rpm	NOUN
mkg-997	98	30	for	for	ADP
mkg-997	98	31	1min	1min	NUM
mkg-997	98	32	.	.	PUNCT
mkg-997	99	1	a	a	DET
mkg-997	99	2	part	part	NOUN
mkg-997	99	3	of	of	ADP
mkg-997	99	4	dna	dna	NOUN
mkg-997	99	5	could	could	AUX
mkg-997	99	6	be	be	AUX
mkg-997	99	7	sequenced	sequence	VERB
mkg-997	99	8	and	and	CCONJ
mkg-997	99	9	the	the	DET
mkg-997	99	10	remaining	remain	VERB
mkg-997	99	11	was	be	AUX
mkg-997	99	12	cloned	clone	VERB
mkg-997	99	13	.	.	PUNCT
mkg-997	100	1	result	result	NOUN
mkg-997	100	2	identification	identification	NOUN
mkg-997	100	3	of	of	ADP
mkg-997	100	4	encoding	encode	VERB
mkg-997	100	5	slpi	slpi	NOUN
mkg-997	100	6	gene	gene	NOUN
mkg-997	100	7	was	be	AUX
mkg-997	100	8	initiated	initiate	VERB
mkg-997	100	9	by	by	ADP
mkg-997	100	10	obtaining	obtain	VERB
mkg-997	100	11	dna	dna	NOUN
mkg-997	100	12	of	of	ADP
mkg-997	100	13	slpi	slpi	NOUN
mkg-997	100	14	,	,	PUNCT
mkg-997	100	15	sample	sample	NOUN
mkg-997	100	16	preparation	preparation	NOUN
mkg-997	100	17	of	of	ADP
mkg-997	100	18	amniotic	amniotic	ADJ
mkg-997	100	19	membrane	membrane	NOUN
mkg-997	100	20	was	be	AUX
mkg-997	100	21	previously	previously	ADV
mkg-997	100	22	done	do	VERB
mkg-997	100	23	to	to	PART
mkg-997	100	24	extract	extract	VERB
mkg-997	100	25	rna	rna	NOUN
mkg-997	100	26	,	,	PUNCT
mkg-997	100	27	than	than	SCONJ
mkg-997	100	28	,	,	PUNCT
mkg-997	100	29	cdna	cdna	PROPN
mkg-997	100	30	slpi	slpi	NOUN
mkg-997	100	31	was	be	AUX
mkg-997	100	32	achieved	achieve	VERB
mkg-997	100	33	.	.	PUNCT
mkg-997	101	1	amplification	amplification	NOUN
mkg-997	101	2	was	be	AUX
mkg-997	101	3	done	do	VERB
mkg-997	101	4	using	use	VERB
mkg-997	101	5	two	two	NUM
mkg-997	101	6	step	step	NOUN
mkg-997	101	7	pcr	pcr	NOUN
mkg-997	101	8	(	(	PUNCT
mkg-997	101	9	qiagen	qiagen	NOUN
mkg-997	101	10	)	)	PUNCT
mkg-997	101	11	method	method	NOUN
mkg-997	101	12	with	with	ADP
mkg-997	101	13	primer	primer	NOUN
mkg-997	101	14	sense	sense	NOUN
mkg-997	101	15	:	:	PUNCT
mkg-997	101	16	5’act	5’act	NUM
mkg-997	101	17	ctt	ctt	NOUN
mkg-997	101	18	gcc	gcc	NOUN
mkg-997	101	19	ttc	ttc	PROPN
mkg-997	101	20	acc	acc	PROPN
mkg-997	101	21	atgaa	atgaa	VERB
mkg-997	101	22	3	3	NUM
mkg-997	101	23	’	'	PUNCT
mkg-997	101	24	and	and	CCONJ
mkg-997	101	25	5’att	5’att	PROPN
mkg-997	101	26	cga	cga	PROPN
mkg-997	101	27	tca	tca	PROPN
mkg-997	101	28	act	act	PROPN
mkg-997	101	29	gga	gga	PROPN
mkg-997	101	30	ctt	ctt	PROPN
mkg-997	101	31	3	3	NUM
mkg-997	101	32	’	'	PUNCT
mkg-997	101	33	,	,	PUNCT
mkg-997	101	34	producing	produce	VERB
mkg-997	101	35	fragment	fragment	NOUN
mkg-997	101	36	of	of	ADP
mkg-997	101	37	dna	dna	PROPN
mkg-997	101	38	530	530	NUM
mkg-997	101	39	bp	bp	NOUN
mkg-997	101	40	.	.	PUNCT
mkg-997	102	1	visualization	visualization	NOUN
mkg-997	102	2	of	of	ADP
mkg-997	102	3	pcr	pcr	ADJ
mkg-997	102	4	product	product	NOUN
mkg-997	102	5	was	be	AUX
mkg-997	102	6	performed	perform	VERB
mkg-997	102	7	using	use	VERB
mkg-997	102	8	agarose	agarose	NOUN
mkg-997	102	9	figure	figure	NOUN
mkg-997	102	10	1	1	NUM
mkg-997	102	11	.	.	PUNCT
mkg-997	103	1	electrophoresis	electrophoresis	NOUN
mkg-997	103	2	of	of	ADP
mkg-997	103	3	pcr	pcr	ADJ
mkg-997	103	4	product	product	NOUN
mkg-997	103	5	to	to	PART
mkg-997	103	6	identify	identify	VERB
mkg-997	103	7	slpi	slpi	NOUN
mkg-997	103	8	gene	gene	NOUN
mkg-997	103	9	.	.	PUNCT
mkg-997	104	1	m	m	PRON
mkg-997	104	2	:	:	PUNCT
mkg-997	104	3	marker	marker	NOUN
mkg-997	104	4	×	×	NOUN
mkg-997	104	5	174	174	NUM
mkg-997	104	6	rf	rf	ADJ
mkg-997	104	7	dna	dna	PROPN
mkg-997	104	8	/	/	SYM
mkg-997	104	9	hae	hae	PROPN
mkg-997	104	10	iii	iii	NUM
mkg-997	104	11	fragments	fragment	NOUN
mkg-997	104	12	;	;	PUNCT
mkg-997	104	13	1	1	NUM
mkg-997	104	14	:	:	PUNCT
mkg-997	104	15	slpi	slpi	NOUN
mkg-997	104	16	.	.	PUNCT
mkg-997	105	1	126	126	NUM
mkg-997	105	2	dent	dent	NOUN
mkg-997	105	3	.	.	PUNCT
mkg-997	106	1	j.	j.	PROPN
mkg-997	106	2	(	(	PUNCT
mkg-997	106	3	maj	maj	PROPN
mkg-997	106	4	.	.	PROPN
mkg-997	106	5	ked	ked	PROPN
mkg-997	106	6	.	.	PUNCT
mkg-997	107	1	gigi	gigi	PROPN
mkg-997	107	2	)	)	PUNCT
mkg-997	107	3	,	,	PUNCT
mkg-997	107	4	vol	vol	NOUN
mkg-997	107	5	.	.	PROPN
mkg-997	108	1	41	41	NUM
mkg-997	108	2	.	.	PUNCT
mkg-997	109	1	no	no	INTJ
mkg-997	109	2	.	.	NOUN
mkg-997	110	1	3	3	NUM
mkg-997	110	2	july	july	PROPN
mkg-997	110	3	–	–	PUNCT
mkg-997	110	4	september	september	PROPN
mkg-997	110	5	2008	2008	NUM
mkg-997	110	6	:	:	PUNCT
mkg-997	110	7	123	123	NUM
mkg-997	110	8	-	-	SYM
mkg-997	110	9	127	127	NUM
mkg-997	110	10	electrophoresis	electrophoresis	NOUN
mkg-997	110	11	supported	support	VERB
mkg-997	110	12	by	by	ADP
mkg-997	110	13	uv	uv	NOUN
mkg-997	110	14	-	-	PUNCT
mkg-997	110	15	transilluminator	transilluminator	NOUN
mkg-997	110	16	and	and	CCONJ
mkg-997	110	17	documented	document	VERB
mkg-997	110	18	by	by	ADP
mkg-997	110	19	polaroid	polaroid	PROPN
mkg-997	110	20	camera	camera	PROPN
mkg-997	110	21	,	,	PUNCT
mkg-997	110	22	as	as	SCONJ
mkg-997	110	23	shown	show	VERB
mkg-997	110	24	on	on	ADP
mkg-997	110	25	figure	figure	NOUN
mkg-997	110	26	1	1	NUM
mkg-997	110	27	.	.	PUNCT
mkg-997	111	1	in	in	ADP
mkg-997	111	2	this	this	DET
mkg-997	111	3	present	present	ADJ
mkg-997	111	4	study	study	NOUN
mkg-997	111	5	identification	identification	NOUN
mkg-997	111	6	of	of	ADP
mkg-997	111	7	amniotic	amniotic	ADJ
mkg-997	111	8	membrane	membrane	NOUN
mkg-997	111	9	slpi	slpi	NOUN
mkg-997	111	10	gene	gene	NOUN
mkg-997	111	11	of	of	ADP
mkg-997	111	12	pcr	pcr	ADJ
mkg-997	111	13	product	product	NOUN
mkg-997	111	14	should	should	AUX
mkg-997	111	15	be	be	AUX
mkg-997	111	16	done	do	VERB
mkg-997	111	17	through	through	ADP
mkg-997	111	18	purification	purification	NOUN
mkg-997	111	19	prior	prior	ADV
mkg-997	111	20	to	to	ADP
mkg-997	111	21	sequencing	sequence	VERB
mkg-997	111	22	and	and	CCONJ
mkg-997	111	23	using	use	VERB
mkg-997	111	24	qiaquuick	qiaquuick	ADJ
mkg-997	111	25	pcr	pcr	NOUN
mkg-997	111	26	purification	purification	NOUN
mkg-997	111	27	kit	kit	NOUN
mkg-997	111	28	(	(	PUNCT
mkg-997	111	29	qiagen	qiagen	PROPN
mkg-997	111	30	)	)	PUNCT
mkg-997	111	31	,	,	PUNCT
mkg-997	111	32	followed	follow	VERB
mkg-997	111	33	by	by	ADP
mkg-997	111	34	uv	uv	NOUN
mkg-997	111	35	-	-	PUNCT
mkg-997	111	36	transluminator	transluminator	NOUN
mkg-997	111	37	detection	detection	NOUN
mkg-997	111	38	,	,	PUNCT
mkg-997	111	39	documented	document	VERB
mkg-997	111	40	using	use	VERB
mkg-997	111	41	polaroid	polaroid	PROPN
mkg-997	111	42	camera	camera	NOUN
mkg-997	111	43	(	(	PUNCT
mkg-997	111	44	figure	figure	NOUN
mkg-997	111	45	2	2	NUM
mkg-997	111	46	)	)	PUNCT
mkg-997	111	47	.	.	PUNCT
mkg-997	112	1	amplification	amplification	NOUN
mkg-997	112	2	of	of	ADP
mkg-997	112	3	sequence	sequence	NOUN
mkg-997	112	4	of	of	ADP
mkg-997	112	5	amniotic	amniotic	ADJ
mkg-997	112	6	membrane	membrane	NOUN
mkg-997	112	7	slpi	slpi	NOUN
mkg-997	112	8	gene	gene	NOUN
mkg-997	112	9	encoding	encode	VERB
mkg-997	112	10	protein	protein	NOUN
mkg-997	112	11	with	with	ADP
mkg-997	112	12	primer	primer	NOUN
mkg-997	112	13	reserve	reserve	NOUN
mkg-997	112	14	cat	cat	PROPN
mkg-997	112	15	tcg	tcg	PROPN
mkg-997	112	16	atc	atc	PROPN
mkg-997	112	17	aac	aac	PROPN
mkg-997	112	18	tgg	tgg	PROPN
mkg-997	112	19	cac	cac	PROPN
mkg-997	112	20	tt	tt	PROPN
mkg-997	112	21	and	and	CCONJ
mkg-997	112	22	primer	primer	VERB
mkg-997	112	23	forward	forward	ADV
mkg-997	112	24	act	act	PROPN
mkg-997	112	25	cct	cct	PROPN
mkg-997	112	26	gcc	gcc	NOUN
mkg-997	112	27	ttc	ttc	PROPN
mkg-997	112	28	acc	acc	PROPN
mkg-997	112	29	atg	atg	PROPN
mkg-997	112	30	aa	aa	PROPN
mkg-997	112	31	(	(	PUNCT
mkg-997	112	32	zhang	zhang	PROPN
mkg-997	112	33	et	et	PROPN
mkg-997	112	34	al	al	PROPN
mkg-997	112	35	,	,	PUNCT
mkg-997	112	36	2001	2001	NUM
mkg-997	112	37	)	)	PUNCT
mkg-997	112	38	producing	produce	VERB
mkg-997	112	39	about	about	ADP
mkg-997	112	40	530	530	NUM
mkg-997	112	41	bp	bp	PROPN
mkg-997	112	42	amplicont	amplicont	PROPN
mkg-997	112	43	as	as	SCONJ
mkg-997	112	44	shown	show	VERB
mkg-997	112	45	on	on	ADP
mkg-997	112	46	figure	figure	NOUN
mkg-997	112	47	3	3	NUM
mkg-997	112	48	.	.	NOUN
mkg-997	112	49	amplification	amplification	NOUN
mkg-997	112	50	of	of	ADP
mkg-997	112	51	amniotic	amniotic	ADJ
mkg-997	112	52	membrane	membrane	NOUN
mkg-997	112	53	slpi	slpi	NOUN
mkg-997	112	54	cdna	cdna	PROPN
mkg-997	112	55	is	be	AUX
mkg-997	112	56	required	require	VERB
mkg-997	112	57	for	for	ADP
mkg-997	112	58	analysis	analysis	NOUN
mkg-997	112	59	of	of	ADP
mkg-997	112	60	amniotic	amniotic	ADJ
mkg-997	112	61	membrane	membrane	NOUN
mkg-997	112	62	slpi	slpi	NOUN
mkg-997	112	63	gene	gene	NOUN
mkg-997	112	64	using	use	VERB
mkg-997	112	65	two	two	NUM
mkg-997	112	66	-	-	PUNCT
mkg-997	112	67	step	step	NOUN
mkg-997	112	68	pcr	pcr	NOUN
mkg-997	112	69	method	method	NOUN
mkg-997	112	70	i.e	i.e	PRON
mkg-997	112	71	rna	rna	NOUN
mkg-997	112	72	isolation	isolation	NOUN
mkg-997	112	73	method	method	NOUN
mkg-997	112	74	was	be	AUX
mkg-997	112	75	previously	previously	ADV
mkg-997	112	76	done	do	VERB
mkg-997	112	77	and	and	CCONJ
mkg-997	112	78	continued	continue	VERB
mkg-997	112	79	by	by	ADP
mkg-997	112	80	cdna	cdna	PROPN
mkg-997	112	81	isolation	isolation	NOUN
mkg-997	112	82	.	.	PUNCT
mkg-997	113	1	cdna	cdna	PROPN
mkg-997	113	2	amplification	amplification	NOUN
mkg-997	113	3	was	be	AUX
mkg-997	113	4	done	do	VERB
mkg-997	113	5	in	in	ADP
mkg-997	113	6	thermal	thermal	ADJ
mkg-997	113	7	cycler	cycler	NOUN
mkg-997	113	8	.	.	PUNCT
mkg-997	114	1	primer	primer	NOUN
mkg-997	114	2	was	be	AUX
mkg-997	114	3	designed	design	VERB
mkg-997	114	4	in	in	ADP
mkg-997	114	5	accordance	accordance	NOUN
mkg-997	114	6	with	with	ADP
mkg-997	114	7	the	the	DET
mkg-997	114	8	criteria	criterion	NOUN
mkg-997	114	9	of	of	ADP
mkg-997	114	10	gene	gene	NOUN
mkg-997	114	11	insertion	insertion	NOUN
mkg-997	114	12	on	on	ADP
mkg-997	114	13	pet	pet	ADJ
mkg-997	114	14	101	101	NUM
mkg-997	114	15	/	/	SYM
mkg-997	114	16	d	d	NOUN
mkg-997	114	17	-	-	PUNCT
mkg-997	114	18	topo	topo	NOUN
mkg-997	114	19	adding	add	VERB
mkg-997	114	20	cacc	cacc	NOUN
mkg-997	114	21	prior	prior	ADV
mkg-997	114	22	to	to	ADP
mkg-997	114	23	atg	atg	PROPN
mkg-997	114	24	(	(	PUNCT
mkg-997	114	25	at	at	ADP
mkg-997	114	26	the	the	DET
mkg-997	114	27	early	early	ADJ
mkg-997	114	28	gene	gene	NOUN
mkg-997	114	29	)	)	PUNCT
mkg-997	114	30	presented	present	VERB
mkg-997	114	31	on	on	ADP
mkg-997	114	32	figure	figure	NOUN
mkg-997	114	33	4	4	NUM
mkg-997	114	34	.	.	PUNCT
mkg-997	114	35	figure	figure	VERB
mkg-997	114	36	3	3	NUM
mkg-997	114	37	.	.	PUNCT
mkg-997	114	38	sequensing	sequense	VERB
mkg-997	114	39	of	of	ADP
mkg-997	114	40	slpi	slpi	NOUN
mkg-997	114	41	gene	gene	NOUN
mkg-997	114	42	using	use	VERB
mkg-997	114	43	forward	forward	ADV
mkg-997	114	44	primer	primer	NOUN
mkg-997	114	45	.	.	PUNCT
mkg-997	115	1	figure	figure	NOUN
mkg-997	115	2	4	4	NUM
mkg-997	115	3	.	.	PUNCT
mkg-997	116	1	primer	primer	NOUN
mkg-997	116	2	design	design	NOUN
mkg-997	116	3	for	for	ADP
mkg-997	116	4	slpi	slpi	NOUN
mkg-997	116	5	gene	gene	NOUN
mkg-997	116	6	amplification	amplification	NOUN
mkg-997	116	7	.	.	PUNCT
mkg-997	117	1	figure	figure	NOUN
mkg-997	117	2	5	5	NUM
mkg-997	117	3	.	.	PUNCT
mkg-997	118	1	the	the	DET
mkg-997	118	2	result	result	NOUN
mkg-997	118	3	of	of	ADP
mkg-997	118	4	electrophoresis	electrophoresis	NOUN
mkg-997	118	5	slpi	slpi	NOUN
mkg-997	118	6	gen	gen	NOUN
mkg-997	118	7	pcr	pcr	ADJ
mkg-997	118	8	product	product	NOUN
mkg-997	118	9	with	with	ADP
mkg-997	118	10	topo	topo	PROPN
mkg-997	118	11	primer	primer	NOUN
mkg-997	118	12	:	:	PUNCT
mkg-997	118	13	m	m	NOUN
mkg-997	118	14	:	:	PUNCT
mkg-997	118	15	marker	marker	NOUN
mkg-997	118	16	×	×	NOUN
mkg-997	118	17	174	174	NUM
mkg-997	118	18	rf	rf	ADJ
mkg-997	118	19	dna	dna	PROPN
mkg-997	118	20	/	/	SYM
mkg-997	118	21	hae	hae	PROPN
mkg-997	118	22	iii	iii	PROPN
mkg-997	118	23	fragment	fragment	NOUN
mkg-997	118	24	,	,	PUNCT
mkg-997	118	25	1	1	NUM
mkg-997	118	26	:	:	PUNCT
mkg-997	118	27	slpi	slpi	NOUN
mkg-997	118	28	figure	figure	NOUN
mkg-997	118	29	6	6	NUM
mkg-997	118	30	.	.	PUNCT
mkg-997	118	31	result	result	NOUN
mkg-997	118	32	of	of	ADP
mkg-997	118	33	slpi	slpi	NOUN
mkg-997	118	34	gene	gene	NOUN
mkg-997	118	35	purification	purification	NOUN
mkg-997	118	36	.	.	PUNCT
mkg-997	119	1	m	m	PRON
mkg-997	119	2	:	:	PUNCT
mkg-997	119	3	marker	marker	NOUN
mkg-997	119	4	×	×	NOUN
mkg-997	119	5	174	174	NUM
mkg-997	119	6	rf	rf	ADJ
mkg-997	119	7	dna	dna	PROPN
mkg-997	119	8	/	/	SYM
mkg-997	119	9	hae	hae	PROPN
mkg-997	119	10	iii	iii	PROPN
mkg-997	119	11	fragment	fragment	NOUN
mkg-997	119	12	,	,	PUNCT
mkg-997	119	13	1	1	NUM
mkg-997	119	14	:	:	PUNCT
mkg-997	119	15	slpi	slpi	NOUN
mkg-997	119	16	5	5	NUM
mkg-997	119	17	'	'	PUNCT
mkg-997	119	18	cacc	cacc	NOUN
mkg-997	119	19	-	-	PUNCT
mkg-997	119	20	atgaagtccagcggcctcttcc	atgaagtccagcggcctcttcc	ADJ
mkg-997	119	21	3	3	NUM
mkg-997	119	22	'	'	PART
mkg-997	119	23	5	5	NUM
mkg-997	119	24	'	'	PART
mkg-997	119	25	agctttcacaggggaaacgcaggat	agctttcacaggggaaacgcaggat	NOUN
mkg-997	119	26	3	3	NUM
mkg-997	119	27	'	'	PART
mkg-997	119	28	figure	figure	NOUN
mkg-997	119	29	2	2	NUM
mkg-997	119	30	.	.	PUNCT
mkg-997	119	31	electrophoresis	electrophoresis	NOUN
mkg-997	119	32	of	of	ADP
mkg-997	119	33	purified	purified	ADJ
mkg-997	119	34	pcr	pcr	ADJ
mkg-997	119	35	product	product	NOUN
mkg-997	119	36	to	to	PART
mkg-997	119	37	identify	identify	VERB
mkg-997	119	38	slpi	slpi	NOUN
mkg-997	119	39	gene	gene	NOUN
mkg-997	119	40	.	.	PUNCT
mkg-997	120	1	m	m	PRON
mkg-997	120	2	:	:	PUNCT
mkg-997	120	3	marker	marker	NOUN
mkg-997	120	4	×	×	NOUN
mkg-997	120	5	174	174	NUM
mkg-997	120	6	rf	rf	ADJ
mkg-997	120	7	dna	dna	PROPN
mkg-997	120	8	/	/	SYM
mkg-997	120	9	hae	hae	PROPN
mkg-997	120	10	iii	iii	NUM
mkg-997	120	11	fragments	fragment	NOUN
mkg-997	120	12	;	;	PUNCT
mkg-997	120	13	1	1	NUM
mkg-997	120	14	:	:	PUNCT
mkg-997	120	15	slpi	slpi	NOUN
mkg-997	120	16	.	.	PUNCT
mkg-997	121	1	amplification	amplification	NOUN
mkg-997	121	2	of	of	ADP
mkg-997	121	3	slpi	slpi	NOUN
mkg-997	121	4	gen	gen	PROPN
mkg-997	121	5	producing	produce	VERB
mkg-997	121	6	dna	dna	NOUN
mkg-997	121	7	fragment	fragment	NOUN
mkg-997	121	8	in	in	ADP
mkg-997	121	9	±	±	NUM
mkg-997	121	10	530	530	NUM
mkg-997	121	11	bp	bp	PROPN
mkg-997	121	12	positions	position	NOUN
mkg-997	121	13	can	can	AUX
mkg-997	121	14	be	be	AUX
mkg-997	121	15	shown	show	VERB
mkg-997	121	16	on	on	ADP
mkg-997	121	17	figure	figure	NOUN
mkg-997	121	18	5	5	NUM
mkg-997	121	19	.	.	PUNCT
mkg-997	121	20	pcr	pcr	ADJ
mkg-997	121	21	products	product	NOUN
mkg-997	121	22	of	of	ADP
mkg-997	121	23	amniotic	amniotic	ADJ
mkg-997	121	24	membrane	membrane	NOUN
mkg-997	121	25	were	be	AUX
mkg-997	121	26	purified	purify	VERB
mkg-997	121	27	using	use	VERB
mkg-997	121	28	topo	topo	PROPN
mkg-997	121	29	primer	primer	NOUN
mkg-997	121	30	prior	prior	ADV
mkg-997	121	31	to	to	ADP
mkg-997	121	32	sequencing	sequence	VERB
mkg-997	121	33	..	..	PUNCT
mkg-997	121	34	band	band	NOUN
mkg-997	121	35	contaminants	contaminant	NOUN
mkg-997	121	36	was	be	AUX
mkg-997	121	37	found	find	VERB
mkg-997	121	38	during	during	ADP
mkg-997	121	39	visualization	visualization	NOUN
mkg-997	121	40	of	of	ADP
mkg-997	121	41	pcr	pcr	ADJ
mkg-997	121	42	product	product	NOUN
mkg-997	121	43	through	through	ADP
mkg-997	121	44	electrophoresis	electrophoresis	NOUN
mkg-997	121	45	,	,	PUNCT
mkg-997	121	46	therefore	therefore	ADV
mkg-997	121	47	prior	prior	ADV
mkg-997	121	48	to	to	ADP
mkg-997	121	49	purification	purification	NOUN
mkg-997	121	50	,	,	PUNCT
mkg-997	121	51	running	run	VERB
mkg-997	121	52	of	of	ADP
mkg-997	121	53	pcr	pcr	ADJ
mkg-997	121	54	product	product	NOUN
mkg-997	121	55	was	be	AUX
mkg-997	121	56	done	do	VERB
mkg-997	121	57	using	use	VERB
mkg-997	121	58	1.5	1.5	NUM
mkg-997	121	59	%	%	NOUN
mkg-997	121	60	lomelting	lomelte	VERB
mkg-997	121	61	agar	agar	NOUN
mkg-997	121	62	.	.	PUNCT
mkg-997	122	1	under	under	ADP
mkg-997	122	2	uv	uv	NOUN
mkg-997	122	3	light	light	NOUN
mkg-997	122	4	,	,	PUNCT
mkg-997	122	5	the	the	DET
mkg-997	122	6	required	require	VERB
mkg-997	122	7	band	band	NOUN
mkg-997	122	8	was	be	AUX
mkg-997	122	9	cut	cut	VERB
mkg-997	122	10	and	and	CCONJ
mkg-997	122	11	mixed	mix	VERB
mkg-997	122	12	with	with	ADP
mkg-997	122	13	qg	qg	PROPN
mkg-997	122	14	buffer	buffer	NOUN
mkg-997	122	15	containing	contain	VERB
mkg-997	122	16	ph	ph	ADJ
mkg-997	122	17	indicator	indicator	NOUN
mkg-997	122	18	.	.	PUNCT
mkg-997	123	1	optimal	optimal	ADJ
mkg-997	123	2	binding	binding	NOUN
mkg-997	123	3	between	between	ADP
mkg-997	123	4	dna	dna	PROPN
mkg-997	123	5	and	and	CCONJ
mkg-997	123	6	silica	silica	NOUN
mkg-997	123	7	gel	gel	NOUN
mkg-997	123	8	requested	request	VERB
mkg-997	123	9	ph	ph	ADJ
mkg-997	123	10	≤	≤	NUM
mkg-997	123	11	7.5	7.5	NUM
mkg-997	123	12	and	and	CCONJ
mkg-997	123	13	would	would	AUX
mkg-997	123	14	produce	produce	VERB
mkg-997	123	15	yellow	yellow	ADJ
mkg-997	123	16	appearance	appearance	NOUN
mkg-997	123	17	,	,	PUNCT
mkg-997	123	18	while	while	SCONJ
mkg-997	123	19	,	,	PUNCT
mkg-997	123	20	purple	purple	ADJ
mkg-997	123	21	appearance	appearance	NOUN
mkg-997	123	22	would	would	AUX
mkg-997	123	23	occur	occur	VERB
mkg-997	123	24	,	,	PUNCT
mkg-997	123	25	ph	ph	PROPN
mkg-997	123	26	was	be	AUX
mkg-997	123	27	too	too	ADV
mkg-997	123	28	high	high	ADJ
mkg-997	123	29	.	.	PUNCT
mkg-997	124	1	after	after	SCONJ
mkg-997	124	2	dna	dna	PROPN
mkg-997	124	3	was	be	AUX
mkg-997	124	4	bound	bind	VERB
mkg-997	124	5	with	with	ADP
mkg-997	124	6	the	the	DET
mkg-997	124	7	membrane	membrane	NOUN
mkg-997	124	8	continued	continue	VERB
mkg-997	124	9	by	by	ADP
mkg-997	124	10	dna	dna	NOUN
mkg-997	124	11	rinsing	rinsing	NOUN
mkg-997	124	12	process	process	NOUN
mkg-997	124	13	by	by	ADP
mkg-997	124	14	releasing	release	VERB
mkg-997	124	15	unnecessary	unnecessary	ADJ
mkg-997	124	16	primer	primer	NOUN
mkg-997	124	17	and	and	CCONJ
mkg-997	124	18	other	other	ADJ
mkg-997	124	19	contaminants	contaminant	NOUN
mkg-997	124	20	.	.	PUNCT
mkg-997	125	1	pe	pe	PROPN
mkg-997	125	2	buffer	buffer	VERB
mkg-997	125	3	additional	additional	ADJ
mkg-997	125	4	containing	contain	VERB
mkg-997	125	5	ethanol	ethanol	NOUN
mkg-997	125	6	would	would	AUX
mkg-997	125	7	make	make	VERB
mkg-997	125	8	contaminants	contaminant	NOUN
mkg-997	125	9	.	.	PUNCT
mkg-997	126	1	flow	flow	VERB
mkg-997	126	2	out	out	ADP
mkg-997	126	3	from	from	ADP
mkg-997	126	4	matrix	matrix	NOUN
mkg-997	126	5	column	column	NOUN
mkg-997	126	6	through	through	ADP
mkg-997	126	7	centrifugation	centrifugation	NOUN
mkg-997	126	8	stage	stage	NOUN
mkg-997	126	9	.	.	PUNCT
mkg-997	127	1	dna	dna	PROPN
mkg-997	127	2	bound	bind	VERB
mkg-997	127	3	with	with	ADP
mkg-997	127	4	membrane	membrane	NOUN
mkg-997	127	5	eluated	eluate	VERB
mkg-997	127	6	by	by	ADP
mkg-997	127	7	adding	add	VERB
mkg-997	127	8	elution	elution	NOUN
mkg-997	127	9	buffer	buffer	NOUN
mkg-997	127	10	exactly	exactly	ADV
mkg-997	127	11	in	in	ADP
mkg-997	127	12	the	the	DET
mkg-997	127	13	middle	middle	NOUN
mkg-997	127	14	of	of	ADP
mkg-997	127	15	membrane	membrane	NOUN
mkg-997	127	16	and	and	CCONJ
mkg-997	127	17	partly	partly	ADV
mkg-997	127	18	purified	purify	VERB
mkg-997	127	19	dna	dna	NOUN
mkg-997	127	20	from	from	ADP
mkg-997	127	21	electrophoresis	electrophoresis	NOUN
mkg-997	127	22	to	to	PART
mkg-997	127	23	identify	identify	VERB
mkg-997	127	24	the	the	DET
mkg-997	127	25	existence	existence	NOUN
mkg-997	127	26	of	of	ADP
mkg-997	127	27	purification	purification	NOUN
mkg-997	127	28	,	,	PUNCT
mkg-997	127	29	followed	follow	VERB
mkg-997	127	30	by	by	ADP
mkg-997	127	31	uv	uv	NOUN
mkg-997	127	32	-	-	PUNCT
mkg-997	127	33	transluminator	transluminator	NOUN
mkg-997	127	34	detection	detection	NOUN
mkg-997	127	35	,	,	PUNCT
mkg-997	127	36	finally	finally	ADV
mkg-997	127	37	,	,	PUNCT
mkg-997	127	38	documented	document	VERB
mkg-997	127	39	by	by	ADP
mkg-997	127	40	polaroid	polaroid	PROPN
mkg-997	127	41	camera	camera	NOUN
mkg-997	127	42	shown	show	VERB
mkg-997	127	43	on	on	ADP
mkg-997	127	44	figure	figure	NOUN
mkg-997	127	45	6	6	NUM
mkg-997	127	46	.	.	PUNCT
mkg-997	128	1	127munadziroh	127munadziroh	NOUN
mkg-997	128	2	:	:	PUNCT
mkg-997	128	3	isolation	isolation	NOUN
mkg-997	128	4	and	and	CCONJ
mkg-997	128	5	identification	identification	NOUN
mkg-997	128	6	of	of	ADP
mkg-997	128	7	java	java	PROPN
mkg-997	128	8	race	race	PROPN
mkg-997	128	9	amniotic	amniotic	ADJ
mkg-997	128	10	membrane	membrane	NOUN
mkg-997	128	11	discussion	discussion	NOUN
mkg-997	128	12	the	the	DET
mkg-997	128	13	existence	existence	NOUN
mkg-997	128	14	of	of	ADP
mkg-997	128	15	amniotic	amniotic	ADJ
mkg-997	128	16	membrane	membrane	NOUN
mkg-997	128	17	spli	spli	NOUN
mkg-997	128	18	gene	gene	NOUN
mkg-997	128	19	was	be	AUX
mkg-997	128	20	detected	detect	VERB
mkg-997	128	21	using	use	VERB
mkg-997	128	22	pcr	pcr	ADJ
mkg-997	128	23	method	method	NOUN
mkg-997	128	24	to	to	PART
mkg-997	128	25	obtain	obtain	VERB
mkg-997	128	26	amplification	amplification	NOUN
mkg-997	128	27	of	of	ADP
mkg-997	128	28	dna	dna	PROPN
mkg-997	128	29	segment	segment	NOUN
mkg-997	128	30	limited	limit	VERB
mkg-997	128	31	by	by	ADP
mkg-997	128	32	synthetic	synthetic	ADJ
mkg-997	128	33	oligonucleotide	oligonucleotide	NOUN
mkg-997	128	34	(	(	PUNCT
mkg-997	128	35	primer	primer	NOUN
mkg-997	128	36	)	)	PUNCT
mkg-997	128	37	.	.	PUNCT
mkg-997	129	1	this	this	PRON
mkg-997	129	2	s	s	VERB
mkg-997	129	3	tudy	tudy	NOUN
mkg-997	129	4	used	use	VERB
mkg-997	129	5	specif	specif	PROPN
mkg-997	129	6	ic	ic	PROPN
mkg-997	129	7	pr	pr	NOUN
mkg-997	129	8	imer	imer	NOUN
mkg-997	129	9	of	of	ADP
mkg-997	129	10	human	human	ADJ
mkg-997	129	11	slpi	slpi	NOUN
mkg-997	129	12	(	(	PUNCT
mkg-997	129	13	5	5	NUM
mkg-997	129	14	’	'	PUNCT
mkg-997	129	15	actcctgccttcaccatgaa3	actcctgccttcaccatgaa3	PROPN
mkg-997	129	16	’	'	PUNCT
mkg-997	129	17	/5	/5	PUNCT
mkg-997	129	18	’	'	PUNCT
mkg-997	129	19	-attcgatcaactggactt-3	-attcgatcaactggactt-3	NOUN
mkg-997	129	20	’	'	PUNCT
mkg-997	129	21	)	)	PUNCT
mkg-997	129	22	.	.	PUNCT
mkg-997	130	1	the	the	DET
mkg-997	130	2	result	result	NOUN
mkg-997	130	3	of	of	ADP
mkg-997	130	4	amplification	amplification	NOUN
mkg-997	130	5	obtained	obtain	VERB
mkg-997	130	6	530	530	NUM
mkg-997	130	7	bp	bp	PROPN
mkg-997	130	8	dna	dna	PROPN
mkg-997	130	9	.	.	PUNCT
mkg-997	131	1	primer	primer	NOUN
mkg-997	131	2	selection	selection	NOUN
mkg-997	131	3	based	base	VERB
mkg-997	131	4	on	on	ADP
mkg-997	131	5	the	the	DET
mkg-997	131	6	study	study	NOUN
mkg-997	131	7	done	do	VERB
mkg-997	131	8	by	by	ADP
mkg-997	131	9	zhang9	zhang9	X
mkg-997	131	10	that	that	SCONJ
mkg-997	131	11	successfully	successfully	ADV
mkg-997	131	12	isolated	isolate	VERB
mkg-997	131	13	slpi	slpi	NOUN
mkg-997	131	14	gene	gene	NOUN
mkg-997	131	15	from	from	ADP
mkg-997	131	16	human	human	ADJ
mkg-997	131	17	amniotic	amniotic	ADJ
mkg-997	131	18	fluid	fluid	NOUN
mkg-997	131	19	by	by	ADP
mkg-997	131	20	rt	rt	NOUN
mkg-997	131	21	-	-	PUNCT
mkg-997	131	22	pcrmethod	pcrmethod	NOUN
mkg-997	131	23	obtaining	obtain	VERB
mkg-997	131	24	570	570	NUM
mkg-997	131	25	bp	bp	PROPN
mkg-997	131	26	slpi	slpi	NOUN
mkg-997	131	27	gene.10	gene.10	NOUN
mkg-997	131	28	homology	homology	NOUN
mkg-997	131	29	analysis	analysis	NOUN
mkg-997	131	30	of	of	ADP
mkg-997	131	31	gene	gene	NOUN
mkg-997	131	32	bank	bank	NOUN
mkg-997	131	33	shows	show	VERB
mkg-997	131	34	human	human	ADJ
mkg-997	131	35	homology	homology	NOUN
mkg-997	131	36	slpi	slpi	NOUN
mkg-997	131	37	indicating	indicate	VERB
mkg-997	131	38	98–100	98–100	NUM
mkg-997	131	39	%	%	NOUN
mkg-997	131	40	homology	homology	NOUN
mkg-997	131	41	,	,	PUNCT
mkg-997	131	42	based	base	VERB
mkg-997	131	43	on	on	ADP
mkg-997	131	44	this	this	DET
mkg-997	131	45	indication	indication	NOUN
mkg-997	131	46	therefore	therefore	ADV
mkg-997	131	47	primer	primer	NOUN
mkg-997	131	48	was	be	AUX
mkg-997	131	49	applied	apply	VERB
mkg-997	131	50	.	.	PUNCT
mkg-997	132	1	some	some	DET
mkg-997	132	2	studies	study	NOUN
mkg-997	132	3	showed	show	VERB
mkg-997	132	4	slpi	slpi	NOUN
mkg-997	132	5	gene	gene	NOUN
mkg-997	132	6	has	have	VERB
mkg-997	132	7	various	various	ADJ
mkg-997	132	8	size	size	NOUN
mkg-997	132	9	.	.	PUNCT
mkg-997	133	1	the	the	DET
mkg-997	133	2	size	size	NOUN
mkg-997	133	3	of	of	ADP
mkg-997	133	4	slpi	slpi	NOUN
mkg-997	133	5	in	in	ADP
mkg-997	133	6	parotid	parotid	ADJ
mkg-997	133	7	gland	gland	NOUN
mkg-997	133	8	is	be	AUX
mkg-997	133	9	580	580	NUM
mkg-997	133	10	bp,11	bp,11	NOUN
mkg-997	133	11	in	in	ADP
mkg-997	133	12	cervical	cervical	ADJ
mkg-997	133	13	mucus	mucus	NOUN
mkg-997	133	14	is	be	AUX
mkg-997	133	15	570	570	NUM
mkg-997	133	16	bp	bp	NOUN
mkg-997	133	17	,	,	PUNCT
mkg-997	133	18	and	and	CCONJ
mkg-997	133	19	in	in	ADP
mkg-997	133	20	endometrial	endometrial	ADJ
mkg-997	133	21	epithelial	epithelial	ADJ
mkg-997	133	22	cell	cell	NOUN
mkg-997	133	23	is	be	AUX
mkg-997	133	24	451	451	NUM
mkg-997	133	25	bp.9	bp.9	NOUN
mkg-997	133	26	various	various	ADJ
mkg-997	133	27	size	size	NOUN
mkg-997	133	28	of	of	ADP
mkg-997	133	29	slpi	slpi	NOUN
mkg-997	133	30	according	accord	VERB
mkg-997	133	31	to	to	ADP
mkg-997	133	32	gene	gene	NOUN
mkg-997	133	33	bank	bank	NOUN
mkg-997	133	34	shown	show	VERB
mkg-997	133	35	on	on	ADP
mkg-997	133	36	table	table	NOUN
mkg-997	133	37	1	1	NUM
mkg-997	133	38	.	.	PUNCT
mkg-997	133	39	alignment	alignment	NOUN
mkg-997	133	40	was	be	AUX
mkg-997	133	41	done	do	VERB
mkg-997	133	42	on	on	ADP
mkg-997	133	43	some	some	DET
mkg-997	133	44	sequence	sequence	NOUN
mkg-997	133	45	of	of	ADP
mkg-997	133	46	nucleotide	nucleotide	NOUN
mkg-997	133	47	obtained	obtain	VERB
mkg-997	133	48	from	from	ADP
mkg-997	133	49	gene	gene	NOUN
mkg-997	133	50	bank	bank	NOUN
mkg-997	133	51	showed	show	VERB
mkg-997	133	52	similar	similar	ADJ
mkg-997	133	53	homology	homology	NOUN
mkg-997	133	54	and	and	CCONJ
mkg-997	133	55	it	it	PRON
mkg-997	133	56	is	be	AUX
mkg-997	133	57	proven	prove	VERB
mkg-997	133	58	that	that	SCONJ
mkg-997	133	59	the	the	DET
mkg-997	133	60	identify	identify	NOUN
mkg-997	133	61	of	of	ADP
mkg-997	133	62	amniotic	amniotic	ADJ
mkg-997	133	63	membrane	membrane	NOUN
mkg-997	133	64	slpi	slpi	NOUN
mkg-997	133	65	gene	gene	NOUN
mkg-997	133	66	is	be	AUX
mkg-997	133	67	similar	similar	ADJ
mkg-997	133	68	to	to	ADP
mkg-997	133	69	human	human	ADJ
mkg-997	133	70	slpi	slpi	NOUN
mkg-997	133	71	registered	register	VERB
mkg-997	133	72	in	in	ADP
mkg-997	133	73	gene	gene	NOUN
mkg-997	133	74	bank	bank	NOUN
mkg-997	133	75	.	.	PUNCT
mkg-997	134	1	amplification	amplification	NOUN
mkg-997	134	2	of	of	ADP
mkg-997	134	3	slpi	slpi	NOUN
mkg-997	134	4	gene	gene	NOUN
mkg-997	134	5	was	be	AUX
mkg-997	134	6	conducted	conduct	VERB
mkg-997	134	7	using	use	VERB
mkg-997	134	8	pcr	pcr	ADJ
mkg-997	134	9	technique	technique	NOUN
mkg-997	134	10	i.e	i.e	X
mkg-997	134	11	in	in	ADP
mkg-997	134	12	vitro	vitro	X
mkg-997	134	13	enzymatic	enzymatic	ADJ
mkg-997	134	14	method	method	NOUN
mkg-997	134	15	producing	produce	VERB
mkg-997	134	16	specific	specific	ADJ
mkg-997	134	17	dna	dna	NOUN
mkg-997	134	18	in	in	ADP
mkg-997	134	19	a	a	DET
mkg-997	134	20	large	large	ADJ
mkg-997	134	21	amount	amount	NOUN
mkg-997	134	22	and	and	CCONJ
mkg-997	134	23	in	in	ADP
mkg-997	134	24	very	very	ADV
mkg-997	134	25	short	short	ADJ
mkg-997	134	26	time	time	NOUN
mkg-997	134	27	through	through	ADP
mkg-997	134	28	the	the	DET
mkg-997	134	29	stage	stage	NOUN
mkg-997	134	30	of	of	ADP
mkg-997	134	31	denaturation	denaturation	NOUN
mkg-997	134	32	,	,	PUNCT
mkg-997	134	33	annealing	annealing	NOUN
mkg-997	134	34	and	and	CCONJ
mkg-997	134	35	extension	extension	NOUN
mkg-997	134	36	at	at	ADP
mkg-997	134	37	different	different	ADJ
mkg-997	134	38	temperature	temperature	NOUN
mkg-997	134	39	.	.	PUNCT
mkg-997	135	1	prior	prior	ADV
mkg-997	135	2	to	to	ADP
mkg-997	135	3	detection	detection	NOUN
mkg-997	135	4	of	of	ADP
mkg-997	135	5	slpi	slpi	NOUN
mkg-997	135	6	existence	existence	NOUN
mkg-997	135	7	,	,	PUNCT
mkg-997	135	8	isolation	isolation	NOUN
mkg-997	135	9	of	of	ADP
mkg-997	135	10	dna	dna	PROPN
mkg-997	135	11	genome	genome	NOUN
mkg-997	135	12	was	be	AUX
mkg-997	135	13	initially	initially	ADV
mkg-997	135	14	conducted	conduct	VERB
mkg-997	135	15	by	by	ADP
mkg-997	135	16	extracting	extract	VERB
mkg-997	135	17	cdna	cdna	PROPN
mkg-997	135	18	and	and	CCONJ
mkg-997	135	19	pcr	pcr	ADJ
mkg-997	135	20	method	method	NOUN
mkg-997	135	21	was	be	AUX
mkg-997	135	22	applied	apply	VERB
mkg-997	135	23	to	to	PART
mkg-997	135	24	achieve	achieve	VERB
mkg-997	135	25	amplification	amplification	NOUN
mkg-997	135	26	of	of	ADP
mkg-997	135	27	a	a	DET
mkg-997	135	28	certain	certain	ADJ
mkg-997	135	29	dna	dna	NOUN
mkg-997	135	30	segment	segment	NOUN
mkg-997	135	31	limited	limit	VERB
mkg-997	135	32	by	by	ADP
mkg-997	135	33	synthetic	synthetic	NOUN
mkg-997	135	34	of	of	ADP
mkg-997	135	35	2	2	NUM
mkg-997	135	36	oligonucleotide	oligonucleotide	NOUN
mkg-997	135	37	called	call	VERB
mkg-997	135	38	primer	primer	NOUN
mkg-997	135	39	.	.	PUNCT
mkg-997	136	1	this	this	DET
mkg-997	136	2	technique	technique	NOUN
mkg-997	136	3	was	be	AUX
mkg-997	136	4	applied	apply	VERB
mkg-997	136	5	due	due	ADP
mkg-997	136	6	to	to	ADP
mkg-997	136	7	the	the	DET
mkg-997	136	8	ability	ability	NOUN
mkg-997	136	9	to	to	PART
mkg-997	136	10	purify	purify	VERB
mkg-997	136	11	dna	dna	NOUN
mkg-997	136	12	polymerase	polymerase	NOUN
mkg-997	136	13	and	and	CCONJ
mkg-997	136	14	to	to	PART
mkg-997	136	15	synthesize	synthesize	VERB
mkg-997	136	16	chemically	chemically	ADV
mkg-997	136	17	dna	dna	PROPN
mkg-997	136	18	oligo	oligo	ADJ
mkg-997	136	19	nucleotide	nucleotide	NOUN
mkg-997	136	20	therefore	therefore	ADV
mkg-997	136	21	;	;	PUNCT
mkg-997	136	22	it	it	PRON
mkg-997	136	23	would	would	AUX
mkg-997	136	24	be	be	AUX
mkg-997	136	25	possible	possible	ADJ
mkg-997	136	26	to	to	PART
mkg-997	136	27	conduct	conduct	VERB
mkg-997	136	28	cloning	cloning	NOUN
mkg-997	136	29	in	in	ADP
mkg-997	136	30	specific	specific	ADJ
mkg-997	136	31	dna	dna	NOUN
mkg-997	136	32	sequence	sequence	NOUN
mkg-997	136	33	that	that	PRON
mkg-997	136	34	is	be	AUX
mkg-997	136	35	amniotic	amniotic	ADJ
mkg-997	136	36	membrane	membrane	NOUN
mkg-997	136	37	slpi	slpi	NOUN
mkg-997	136	38	gen	gen	PROPN
mkg-997	136	39	.	.	PROPN
mkg-997	136	40	zhang8	zhang8	PROPN
mkg-997	136	41	suggested	suggest	VERB
mkg-997	136	42	the	the	DET
mkg-997	136	43	production	production	NOUN
mkg-997	136	44	of	of	ADP
mkg-997	136	45	human	human	ADJ
mkg-997	136	46	amniotic	amniotic	ADJ
mkg-997	136	47	membrane	membrane	NOUN
mkg-997	136	48	slpi	slpi	NOUN
mkg-997	136	49	gene	gene	NOUN
mkg-997	136	50	and	and	CCONJ
mkg-997	136	51	regulating	regulate	VERB
mkg-997	136	52	concentration	concentration	NOUN
mkg-997	136	53	of	of	ADP
mkg-997	136	54	amniotic	amniotic	ADJ
mkg-997	136	55	fluid	fluid	NOUN
mkg-997	136	56	through	through	ADP
mkg-997	136	57	pcr	pcr	ADJ
mkg-997	136	58	amplification	amplification	NOUN
mkg-997	136	59	stated	state	VERB
mkg-997	136	60	that	that	SCONJ
mkg-997	136	61	the	the	DET
mkg-997	136	62	length	length	NOUN
mkg-997	136	63	of	of	ADP
mkg-997	136	64	nucleotide	nucleotide	NOUN
mkg-997	136	65	of	of	ADP
mkg-997	136	66	amniotic	amniotic	ADJ
mkg-997	136	67	membrane	membrane	NOUN
mkg-997	136	68	slpi	slpi	NOUN
mkg-997	136	69	gene	gene	NOUN
mkg-997	136	70	is	be	AUX
mkg-997	136	71	570	570	NUM
mkg-997	136	72	pb	pb	NOUN
mkg-997	136	73	.	.	PUNCT
mkg-997	137	1	however	however	ADV
mkg-997	137	2	,	,	PUNCT
mkg-997	137	3	sequence	sequence	NOUN
mkg-997	137	4	data	datum	NOUN
mkg-997	137	5	of	of	ADP
mkg-997	137	6	amniotic	amniotic	ADJ
mkg-997	137	7	membrane	membrane	NOUN
mkg-997	137	8	slpi	slpi	NOUN
mkg-997	137	9	nucleotide	nucleotide	NOUN
mkg-997	137	10	is	be	AUX
mkg-997	137	11	not	not	PART
mkg-997	137	12	found	find	VERB
mkg-997	137	13	at	at	ADP
mkg-997	137	14	gene	gene	NOUN
mkg-997	137	15	bank	bank	NOUN
mkg-997	137	16	.	.	PUNCT
mkg-997	138	1	it	it	PRON
mkg-997	138	2	was	be	AUX
mkg-997	138	3	reported	report	VERB
mkg-997	138	4	that	that	SCONJ
mkg-997	138	5	expressed	express	VERB
mkg-997	138	6	slpi	slpi	NOUN
mkg-997	138	7	in	in	ADP
mkg-997	138	8	parotic	parotic	ADJ
mkg-997	138	9	gland	gland	NOUN
mkg-997	138	10	is	be	AUX
mkg-997	138	11	399	399	NUM
mkg-997	138	12	pb	pb	ADP
mkg-997	138	13	,	,	PUNCT
mkg-997	138	14	while	while	SCONJ
mkg-997	138	15	,	,	PUNCT
mkg-997	138	16	in	in	ADP
mkg-997	138	17	this	this	DET
mkg-997	138	18	study	study	NOUN
mkg-997	138	19	,	,	PUNCT
mkg-997	138	20	slpi	slpi	NOUN
mkg-997	138	21	gene	gene	NOUN
mkg-997	138	22	is	be	AUX
mkg-997	138	23	530	530	NUM
mkg-997	138	24	pb.1	pb.1	NOUN
mkg-997	138	25	if	if	SCONJ
mkg-997	138	26	the	the	DET
mkg-997	138	27	alignment	alignment	NOUN
mkg-997	138	28	between	between	ADP
mkg-997	138	29	sequence	sequence	NOUN
mkg-997	138	30	result	result	NOUN
mkg-997	138	31	of	of	ADP
mkg-997	138	32	this	this	DET
mkg-997	138	33	study	study	NOUN
mkg-997	138	34	and	and	CCONJ
mkg-997	138	35	the	the	DET
mkg-997	138	36	result	result	NOUN
mkg-997	138	37	of	of	ADP
mkg-997	138	38	study	study	NOUN
mkg-997	138	39	done	do	VERB
mkg-997	138	40	by	by	ADP
mkg-997	138	41	stedler,9	stedler,9	NOUN
mkg-997	138	42	98	98	NUM
mkg-997	138	43	%	%	NOUN
mkg-997	138	44	homology	homology	NOUN
mkg-997	138	45	was	be	AUX
mkg-997	138	46	found	find	VERB
mkg-997	138	47	.	.	PUNCT
mkg-997	139	1	if	if	SCONJ
mkg-997	139	2	the	the	DET
mkg-997	139	3	alignment	alignment	NOUN
mkg-997	139	4	was	be	AUX
mkg-997	139	5	done	do	VERB
mkg-997	139	6	only	only	ADV
mkg-997	139	7	cds	cd	NOUN
mkg-997	139	8	and	and	CCONJ
mkg-997	139	9	the	the	DET
mkg-997	139	10	alignment	alignment	NOUN
mkg-997	139	11	found	find	VERB
mkg-997	139	12	100	100	NUM
mkg-997	139	13	%	%	NOUN
mkg-997	139	14	it	it	PRON
mkg-997	139	15	shows	show	VERB
mkg-997	139	16	slpi	slpi	NOUN
mkg-997	139	17	gene	gene	NOUN
mkg-997	139	18	has	have	VERB
mkg-997	139	19	the	the	DET
mkg-997	139	20	same	same	ADJ
mkg-997	139	21	conserve	conserve	NOUN
mkg-997	139	22	area	area	NOUN
mkg-997	139	23	but	but	CCONJ
mkg-997	139	24	the	the	DET
mkg-997	139	25	gene	gene	NOUN
mkg-997	139	26	size	size	NOUN
mkg-997	139	27	is	be	AUX
mkg-997	139	28	not	not	PART
mkg-997	139	29	the	the	DET
mkg-997	139	30	same	same	ADJ
mkg-997	139	31	.	.	PUNCT
mkg-997	140	1	the	the	DET
mkg-997	140	2	conclusion	conclusion	NOUN
mkg-997	140	3	of	of	ADP
mkg-997	140	4	this	this	DET
mkg-997	140	5	study	study	NOUN
mkg-997	140	6	,	,	PUNCT
mkg-997	140	7	human	human	ADJ
mkg-997	140	8	slpi	slpi	NOUN
mkg-997	140	9	are	be	AUX
mkg-997	140	10	highly	highly	ADV
mkg-997	140	11	conserved	conserved	ADJ
mkg-997	140	12	in	in	ADP
mkg-997	140	13	sequence	sequence	NOUN
mkg-997	140	14	content	content	NOUN
mkg-997	140	15	as	as	SCONJ
mkg-997	140	16	compared	compare	VERB
mkg-997	140	17	to	to	ADP
mkg-997	140	18	the	the	DET
mkg-997	140	19	human	human	ADJ
mkg-997	140	20	slpi	slpi	NOUN
mkg-997	140	21	from	from	ADP
mkg-997	140	22	gene	gene	NOUN
mkg-997	140	23	.	.	PUNCT
mkg-997	141	1	references	reference	NOUN
mkg-997	141	2	1	1	NUM
mkg-997	141	3	.	.	PUNCT
mkg-997	142	1	kassab	kassab	PROPN
mkg-997	142	2	mm	mm	PROPN
mkg-997	142	3	,	,	PUNCT
mkg-997	142	4	cohen	cohen	PROPN
mkg-997	142	5	re	re	PROPN
mkg-997	142	6	.	.	PUNCT
mkg-997	143	1	the	the	DET
mkg-997	143	2	etyology	etyology	NOUN
mkg-997	143	3	and	and	CCONJ
mkg-997	143	4	prevalence	prevalence	NOUN
mkg-997	143	5	of	of	ADP
mkg-997	143	6	gingival	gingival	NOUN
mkg-997	143	7	recession	recession	NOUN
mkg-997	143	8	.	.	PUNCT
mkg-997	144	1	j	j	PROPN
mkg-997	144	2	am	be	AUX
mkg-997	144	3	dent	dent	ADJ
mkg-997	144	4	asso	asso	NOUN
mkg-997	144	5	2003	2003	NUM
mkg-997	144	6	;	;	PUNCT
mkg-997	144	7	134(2):220–25	134(2):220–25	NUM
mkg-997	144	8	.	.	X
mkg-997	145	1	2	2	X
mkg-997	145	2	.	.	NUM
mkg-997	145	3	nurman	nurman	PROPN
mkg-997	145	4	mg	mg	PROPN
mkg-997	145	5	,	,	PUNCT
mkg-997	145	6	takei	takei	PROPN
mkg-997	145	7	hh	hh	PROPN
mkg-997	145	8	,	,	PUNCT
mkg-997	145	9	carranza	carranza	PROPN
mkg-997	145	10	fa	fa	PROPN
mkg-997	145	11	.	.	PUNCT
mkg-997	145	12	carranza	carranza	PROPN
mkg-997	145	13	’s	’s	PART
mkg-997	145	14	clinical	clinical	ADJ
mkg-997	145	15	periodontology	periodontology	NOUN
mkg-997	145	16	.	.	PUNCT
mkg-997	146	1	9th	9th	ADJ
mkg-997	146	2	ed	ed	NOUN
mkg-997	146	3	.	.	PUNCT
mkg-997	146	4	philadelphia	philadelphia	PROPN
mkg-997	146	5	:	:	PUNCT
mkg-997	146	6	wb	wb	PROPN
mkg-997	146	7	saunders	saunders	PROPN
mkg-997	146	8	company	company	NOUN
mkg-997	146	9	;	;	PUNCT
mkg-997	146	10	2002	2002	NUM
mkg-997	146	11	.	.	PUNCT
mkg-997	147	1	p.	p.	NOUN
mkg-997	147	2	651–61	651–61	NUM
mkg-997	147	3	.	.	PUNCT
mkg-997	148	1	3	3	X
mkg-997	148	2	.	.	X
mkg-997	148	3	goldman	goldman	PROPN
mkg-997	148	4	lmd	lmd	PROPN
mkg-997	148	5	,	,	PUNCT
mkg-997	148	6	ausiello	ausiello	PROPN
mkg-997	148	7	dmd	dmd	PROPN
mkg-997	148	8	.	.	PROPN
mkg-997	148	9	2004	2004	NUM
mkg-997	148	10	.	.	PUNCT
mkg-997	149	1	cecil	cecil	PROPN
mkg-997	149	2	textbook	textbook	NOUN
mkg-997	149	3	of	of	ADP
mkg-997	149	4	medicine	medicine	NOUN
mkg-997	149	5	.	.	PUNCT
mkg-997	150	1	22nd	22nd	NOUN
mkg-997	150	2	ed	ed	NOUN
mkg-997	150	3	.	.	PUNCT
mkg-997	150	4	philadelphia	philadelphia	PROPN
mkg-997	150	5	:	:	PUNCT
mkg-997	150	6	wb	wb	PROPN
mkg-997	150	7	saunders	saunder	NOUN
mkg-997	150	8	co	co	PROPN
mkg-997	150	9	;	;	PUNCT
mkg-997	150	10	p.	p.	PROPN
mkg-997	150	11	208–33	208–33	NUM
mkg-997	150	12	.	.	PUNCT
mkg-997	151	1	4	4	NUM
mkg-997	151	2	.	.	X
mkg-997	151	3	senthil	senthil	PROPN
mkg-997	151	4	k	k	PROPN
mkg-997	151	5	,	,	PUNCT
mkg-997	151	6	wong	wong	PROPN
mkg-997	151	7	peng	peng	PROPN
mkg-997	151	8	foo	foo	PROPN
mkg-997	151	9	,	,	PUNCT
mkg-997	151	10	david	david	PROPN
mkg-997	151	11	lj	lj	PROPN
mkg-997	151	12	.	.	PUNCT
mkg-997	152	1	what	what	PRON
mkg-997	152	2	is	be	AUX
mkg-997	152	3	new	new	ADJ
mkg-997	152	4	in	in	ADP
mkg-997	152	5	wound	wound	NOUN
mkg-997	152	6	healing	healing	NOUN
mkg-997	152	7	?	?	PUNCT
mkg-997	152	8	.	.	PUNCT
mkg-997	153	1	turk	turk	PROPN
mkg-997	153	2	j	j	PROPN
mkg-997	153	3	med	med	PROPN
mkg-997	153	4	sci	sci	PROPN
mkg-997	153	5	2004	2004	NUM
mkg-997	153	6	;	;	PUNCT
mkg-997	153	7	34	34	NUM
mkg-997	153	8	:	:	PUNCT
mkg-997	153	9	147–60	147–60	NUM
mkg-997	153	10	.	.	PROPN
mkg-997	153	11	5	5	NUM
mkg-997	153	12	.	.	X
mkg-997	153	13	ley	ley	PROPN
mkg-997	153	14	-	-	PUNCT
mkg-997	153	15	chavez	chavez	PROPN
mkg-997	153	16	e	e	PROPN
mkg-997	153	17	,	,	PUNCT
mkg-997	153	18	martinez	martinez	PROPN
mkg-997	153	19	-	-	PUNCT
mkg-997	153	20	pardo	pardo	PROPN
mkg-997	153	21	me	me	PROPN
mkg-997	153	22	,	,	PUNCT
mkg-997	153	23	roman	roman	ADJ
mkg-997	153	24	r	r	NOUN
mkg-997	153	25	,	,	PUNCT
mkg-997	153	26	oliveros	oliveros	PROPN
mkg-997	153	27	-	-	PUNCT
mkg-997	153	28	lozano	lozano	PROPN
mkg-997	153	29	fj	fj	PROPN
mkg-997	153	30	,	,	PUNCT
mkg-997	153	31	canchola	canchola	PROPN
mkg-997	153	32	-	-	PUNCT
mkg-997	153	33	martinez	martinez	PROPN
mkg-997	153	34	.	.	PUNCT
mkg-997	154	1	application	application	NOUN
mkg-997	154	2	of	of	ADP
mkg-997	154	3	biological	biological	ADJ
mkg-997	154	4	dressings	dressing	NOUN
mkg-997	154	5	from	from	ADP
mkg-997	154	6	radiosterilized	radiosterilized	ADJ
mkg-997	154	7	amnios	amnio	NOUN
mkg-997	154	8	with	with	ADP
mkg-997	154	9	cobalt	cobalt	NOUN
mkg-997	154	10	60	60	NUM
mkg-997	154	11	and	and	CCONJ
mkg-997	154	12	serologic	serologic	VERB
mkg-997	154	13	studies	study	NOUN
mkg-997	154	14	on	on	ADP
mkg-997	154	15	the	the	DET
mkg-997	154	16	handling	handling	NOUN
mkg-997	154	17	of	of	ADP
mkg-997	154	18	burns	burn	NOUN
mkg-997	154	19	in	in	ADP
mkg-997	154	20	pediatric	pediatric	ADJ
mkg-997	154	21	patients	patient	NOUN
mkg-997	154	22	.	.	PUNCT
mkg-997	155	1	ann	ann	PROPN
mkg-997	155	2	transplant	transplant	PROPN
mkg-997	155	3	2003	2003	NUM
mkg-997	155	4	;	;	PUNCT
mkg-997	155	5	8(4	8(4	NUM
mkg-997	155	6	):	):	PUNCT
mkg-997	155	7	46–9	46–9	NUM
mkg-997	155	8	.	.	NOUN
mkg-997	156	1	6	6	NUM
mkg-997	156	2	.	.	X
mkg-997	157	1	heinz	heinz	PROPN
mkg-997	157	2	c	c	PROPN
mkg-997	157	3	,	,	PUNCT
mkg-997	157	4	eckstein	eckstein	PROPN
mkg-997	157	5	a	a	PRON
mkg-997	157	6	,	,	PUNCT
mkg-997	157	7	steuhl	steuhl	PROPN
mkg-997	157	8	kp	kp	PROPN
mkg-997	157	9	,	,	PUNCT
mkg-997	157	10	meller	meller	PROPN
mkg-997	157	11	d.	d.	PROPN
mkg-997	157	12	amniotic	amniotic	ADJ
mkg-997	157	13	membrane	membrane	NOUN
mkg-997	157	14	transplantation	transplantation	NOUN
mkg-997	157	15	for	for	ADP
mkg-997	157	16	reconstruction	reconstruction	NOUN
mkg-997	157	17	of	of	ADP
mkg-997	157	18	corneal	corneal	ADJ
mkg-997	157	19	ulcer	ulcer	NOUN
mkg-997	157	20	in	in	ADP
mkg-997	157	21	graves	grave	NOUN
mkg-997	157	22	ophthalmopathy	ophthalmopathy	ADJ
mkg-997	157	23	.	.	PUNCT
mkg-997	158	1	journal	journal	NOUN
mkg-997	158	2	of	of	ADP
mkg-997	158	3	cornea	cornea	PROPN
mkg-997	158	4	2004	2004	NUM
mkg-997	158	5	;	;	PUNCT
mkg-997	158	6	23(5):524–26	23(5):524–26	NUM
mkg-997	158	7	.	.	PUNCT
mkg-997	159	1	7	7	X
mkg-997	159	2	.	.	X
mkg-997	159	3	takahiro	takahiro	PROPN
mkg-997	159	4	n	n	CCONJ
mkg-997	159	5	,	,	PUNCT
mkg-997	159	6	makoto	makoto	PROPN
mkg-997	159	7	y	y	PROPN
mkg-997	159	8	,	,	PUNCT
mkg-997	159	9	helen	helen	PROPN
mkg-997	159	10	r.	r.	PROPN
mkg-997	159	11	sterilized	sterilize	VERB
mkg-997	159	12	,	,	PUNCT
mkg-997	159	13	freeze	freeze	NOUN
mkg-997	159	14	-	-	PUNCT
mkg-997	159	15	dried	dry	VERB
mkg-997	159	16	amniotic	amniotic	ADJ
mkg-997	159	17	membrane	membrane	NOUN
mkg-997	159	18	:	:	PUNCT
mkg-997	159	19	a	a	DET
mkg-997	159	20	useful	useful	ADJ
mkg-997	159	21	substrate	substrate	NOUN
mkg-997	159	22	for	for	ADP
mkg-997	159	23	ocular	ocular	ADJ
mkg-997	159	24	surface	surface	NOUN
mkg-997	159	25	reconstruction	reconstruction	NOUN
mkg-997	159	26	.	.	PUNCT
mkg-997	160	1	investigative	investigative	ADJ
mkg-997	160	2	ophthalmology	ophthalmology	NOUN
mkg-997	160	3	and	and	CCONJ
mkg-997	160	4	visual	visual	ADJ
mkg-997	160	5	science	science	NOUN
mkg-997	160	6	2004	2004	NUM
mkg-997	160	7	;	;	PUNCT
mkg-997	160	8	45:93–9	45:93–9	NUM
mkg-997	160	9	.	.	PUNCT
mkg-997	161	1	8	8	X
mkg-997	161	2	.	.	X
mkg-997	161	3	fitch	fitch	PROPN
mkg-997	161	4	pm	pm	PROPN
mkg-997	161	5	,	,	PUNCT
mkg-997	161	6	roghanian	roghanian	PROPN
mkg-997	161	7	a	a	PRON
mkg-997	161	8	,	,	PUNCT
mkg-997	161	9	howie	howie	PROPN
mkg-997	161	10	sem	sem	PROPN
mkg-997	161	11	,	,	PUNCT
mkg-997	161	12	sallenave	sallenave	PROPN
mkg-997	161	13	jm	jm	PROPN
mkg-997	161	14	.	.	PROPN
mkg-997	161	15	human	human	ADJ
mkg-997	161	16	neutrophil	neutrophil	ADJ
mkg-997	161	17	elastase	elastase	VERB
mkg-997	161	18	inhibitors	inhibitor	NOUN
mkg-997	161	19	in	in	ADP
mkg-997	161	20	innate	innate	ADJ
mkg-997	161	21	and	and	CCONJ
mkg-997	161	22	adaptive	adaptive	ADJ
mkg-997	161	23	immunity	immunity	NOUN
mkg-997	161	24	.	.	PUNCT
mkg-997	162	1	biohem	biohem	PROPN
mkg-997	162	2	soc	soc	PROPN
mkg-997	162	3	trans	trans	PROPN
mkg-997	162	4	2006	2006	NUM
mkg-997	162	5	;	;	PUNCT
mkg-997	162	6	34:279–82	34:279–82	NUM
mkg-997	162	7	.	.	PUNCT
mkg-997	163	1	9	9	NUM
mkg-997	163	2	.	.	X
mkg-997	163	3	zhang	zhang	PROPN
mkg-997	163	4	daying	daying	PROPN
mkg-997	163	5	,	,	PUNCT
mkg-997	163	6	rosalia	rosalia	PROPN
mkg-997	163	7	cm	cm	PROPN
mkg-997	163	8	,	,	PUNCT
mkg-997	163	9	simmen	simman	NOUN
mkg-997	163	10	,	,	PUNCT
mkg-997	163	11	frank	frank	PROPN
mkg-997	163	12	j.	j.	PROPN
mkg-997	163	13	michel	michel	PROPN
mkg-997	163	14	,	,	PUNCT
mkg-997	163	15	ge	ge	PROPN
mkg-997	163	16	zhao	zhao	PROPN
mkg-997	163	17	,	,	PUNCT
mkg-997	163	18	dustin	dustin	PROPN
mkg-997	163	19	vale	vale	PROPN
mkg-997	163	20	-	-	PUNCT
mkg-997	163	21	cruz	cruz	NOUN
mkg-997	163	22	,	,	PUNCT
mkg-997	163	23	et	et	PROPN
mkg-997	163	24	al	al	PROPN
mkg-997	163	25	.	.	PUNCT
mkg-997	163	26	secretory	secretory	ADJ
mkg-997	163	27	leukocyte	leukocyte	NOUN
mkg-997	163	28	protease	protease	NOUN
mkg-997	163	29	inhibitor	inhibitor	NOUN
mkg-997	163	30	mediates	mediate	NOUN
mkg-997	163	31	proliferation	proliferation	NOUN
mkg-997	163	32	of	of	ADP
mkg-997	163	33	human	human	ADJ
mkg-997	163	34	endometrial	endometrial	ADJ
mkg-997	163	35	epithelial	epithelial	ADJ
mkg-997	163	36	cells	cell	NOUN
mkg-997	163	37	by	by	ADP
mkg-997	163	38	positive	positive	ADJ
mkg-997	163	39	and	and	CCONJ
mkg-997	163	40	negative	negative	ADJ
mkg-997	163	41	regulation	regulation	NOUN
mkg-997	163	42	of	of	ADP
mkg-997	163	43	growth	growth	NOUN
mkg-997	163	44	-	-	PUNCT
mkg-997	163	45	associated	associate	VERB
mkg-997	163	46	genes	gene	NOUN
mkg-997	163	47	.	.	PUNCT
mkg-997	164	1	journal	journal	NOUN
mkg-997	164	2	of	of	ADP
mkg-997	164	3	biological	biological	ADJ
mkg-997	164	4	chemistry	chemistry	NOUN
mkg-997	164	5	2002	2002	NUM
mkg-997	164	6	;	;	PUNCT
mkg-997	164	7	277	277	NUM
mkg-997	164	8	(	(	PUNCT
mkg-997	164	9	33	33	NUM
mkg-997	164	10	):	):	PUNCT
mkg-997	164	11	29999–30009	29999–30009	NUM
mkg-997	164	12	.	.	PUNCT
mkg-997	165	1	10	10	NUM
mkg-997	165	2	.	.	PUNCT
mkg-997	166	1	zhang	zhang	PROPN
mkg-997	166	2	qing	qing	PROPN
mkg-997	166	3	,	,	PUNCT
mkg-997	166	4	koichiro	koichiro	PROPN
mkg-997	166	5	shimoya	shimoya	PROPN
mkg-997	166	6	,	,	PUNCT
mkg-997	166	7	akihiro	akihiro	PROPN
mkg-997	166	8	moriyama	moriyama	PROPN
mkg-997	166	9	,	,	PUNCT
mkg-997	166	10	kaoru	kaoru	PROPN
mkg-997	166	11	yamanaka	yamanaka	PROPN
mkg-997	166	12	,	,	PUNCT
mkg-997	166	13	atsuko	atsuko	PROPN
mkg-997	166	14	nakajima	nakajima	PROPN
mkg-997	166	15	,	,	PUNCT
mkg-997	166	16	toshikatsu	toshikatsu	NOUN
mkg-997	166	17	nobunaga	nobunaga	NOUN
mkg-997	166	18	,	,	PUNCT
mkg-997	166	19	et	et	PROPN
mkg-997	166	20	al	al	PROPN
mkg-997	166	21	.	.	PUNCT
mkg-997	166	22	production	production	NOUN
mkg-997	166	23	of	of	ADP
mkg-997	166	24	secretory	secretory	ADJ
mkg-997	166	25	leukocyte	leukocyte	NOUN
mkg-997	166	26	protease	protease	NOUN
mkg-997	166	27	inhibitor	inhibitor	NOUN
mkg-997	166	28	by	by	ADP
mkg-997	166	29	human	human	ADJ
mkg-997	166	30	amniotic	amniotic	ADJ
mkg-997	166	31	membranes	membrane	NOUN
mkg-997	166	32	and	and	CCONJ
mkg-997	166	33	regulation	regulation	NOUN
mkg-997	166	34	of	of	ADP
mkg-997	166	35	its	its	PRON
mkg-997	166	36	concentration	concentration	NOUN
mkg-997	166	37	in	in	ADP
mkg-997	166	38	amniotic	amniotic	ADJ
mkg-997	166	39	fluid	fluid	NOUN
mkg-997	166	40	.	.	PUNCT
mkg-997	167	1	molecular	molecular	ADJ
mkg-997	167	2	human	human	ADJ
mkg-997	167	3	reproduction	reproduction	NOUN
mkg-997	167	4	2001	2001	NUM
mkg-997	167	5	;	;	PUNCT
mkg-997	167	6	7(6):573–79	7(6):573–79	ADV
mkg-997	167	7	.	.	PUNCT
mkg-997	168	1	11	11	NUM
mkg-997	168	2	.	.	PUNCT
mkg-997	169	1	stedler	stedler	NOUN
mkg-997	169	2	g	g	PROPN
mkg-997	169	3	,	,	PUNCT
mkg-997	169	4	m	m	VERB
mkg-997	169	5	t	t	NOUN
mkg-997	169	6	brewer	brewer	NOUN
mkg-997	169	7	,	,	PUNCT
mkg-997	169	8	thompson	thompson	PROPN
mkg-997	169	9	rc	rc	PROPN
mkg-997	169	10	.	.	PROPN
mkg-997	169	11	isolation	isolation	NOUN
mkg-997	169	12	and	and	CCONJ
mkg-997	169	13	sequence	sequence	NOUN
mkg-997	169	14	of	of	ADP
mkg-997	169	15	a	a	DET
mkg-997	169	16	human	human	ADJ
mkg-997	169	17	gene	gene	NOUN
mkg-997	169	18	encoding	encode	VERB
mkg-997	169	19	a	a	DET
mkg-997	169	20	potent	potent	ADJ
mkg-997	169	21	inhibitor	inhibitor	NOUN
mkg-997	169	22	of	of	ADP
mkg-997	169	23	leukocyte	leukocyte	ADJ
mkg-997	169	24	proteases	protease	NOUN
mkg-997	169	25	.	.	PUNCT
mkg-997	170	1	nucleic	nucleic	ADJ
mkg-997	170	2	acids	acid	NOUN
mkg-997	170	3	res	re	NOUN
mkg-997	170	4	1986	1986	NUM
mkg-997	170	5	;	;	PUNCT
mkg-997	170	6	14	14	NUM
mkg-997	170	7	(	(	PUNCT
mkg-997	170	8	20):7883–96	20):7883–96	NUM
mkg-997	170	9	.	.	PUNCT
mkg-997	170	10	table	table	NOUN
mkg-997	170	11	1	1	NUM
mkg-997	170	12	.	.	PUNCT
mkg-997	170	13	gene	gene	PROPN
mkg-997	170	14	bank	bank	PROPN
mkg-997	170	15	’s	’s	PART
mkg-997	170	16	list	list	NOUN
mkg-997	170	17	of	of	ADP
mkg-997	170	18	slpi	slpi	NOUN
mkg-997	170	19	size	size	NOUN
mkg-997	170	20	no	no	DET
mkg-997	170	21	accession	accession	NOUN
mkg-997	170	22	number	number	NOUN
mkg-997	170	23	gen	gen	NOUN
mkg-997	170	24	size	size	NOUN
mkg-997	170	25	(	(	PUNCT
mkg-997	170	26	pb	pb	NOUN
mkg-997	170	27	)	)	PUNCT
mkg-997	170	28	source	source	NOUN
mkg-997	170	29	of	of	ADP
mkg-997	170	30	slpi	slpi	NOUN
mkg-997	170	31	1	1	NUM
mkg-997	170	32	2	2	NUM
mkg-997	170	33	3	3	NUM
mkg-997	170	34	4	4	NUM
mkg-997	170	35	5	5	NUM
mkg-997	170	36	6	6	NUM
mkg-997	170	37	7	7	NUM
mkg-997	170	38	8	8	NUM
mkg-997	170	39	9	9	NUM
mkg-997	170	40	10	10	NUM
mkg-997	170	41	x04502	x04502	X
mkg-997	171	1	m74444	m74444	ADJ
mkg-997	171	2	bc020708	bc020708	ADV
mkg-997	171	3	nm003064	nm003064	ADJ
mkg-997	171	4	cs106326	cs106326	NOUN
mkg-997	172	1	x04470	x04470	PROPN
mkg-997	173	1	x04503	x04503	PROPN
mkg-997	173	2	dq891365	dq891365	PROPN
mkg-997	173	3	dq894545	dq894545	PROPN
mkg-997	173	4	af114471	af114471	PROPN
mkg-997	173	5	2657	2657	NUM
mkg-997	173	6	1472	1472	NUM
mkg-997	173	7	625	625	NUM
mkg-997	173	8	598	598	NUM
mkg-997	173	9	594	594	NUM
mkg-997	173	10	594	594	NUM
mkg-997	173	11	573	573	NUM
mkg-997	173	12	439	439	NUM
mkg-997	173	13	439	439	NUM
mkg-997	173	14	399	399	NUM
mkg-997	173	15	parotid	parotid	ADJ
mkg-997	173	16	tissue	tissue	NOUN
mkg-997	173	17	epithelium	epithelium	NOUN
mkg-997	173	18	cell	cell	NOUN
mkg-997	173	19	endometrial	endometrial	ADJ
mkg-997	173	20	cell	cell	NOUN
mkg-997	173	21	cervical	cervical	ADJ
mkg-997	173	22	uterus	uterus	NOUN
mkg-997	173	23	parotid	parotid	ADJ
mkg-997	173	24	tissue	tissue	NOUN
mkg-997	173	25	synthesis	synthesis	NOUN
mkg-997	173	26	synthesis	synthesis	NOUN
mkg-997	173	27	intestinal	intestinal	ADJ
mkg-997	173	28	epithelium	epithelium	NOUN
mkg-997	173	29	cell	cell	NOUN