Dermatology: Practical and Conceptual Observation | Dermatol Pract Concept 2017;7(2):10 47 DERMATOLOGY PRACTICAL & CONCEPTUAL www.derm101.com Case Presentation A 57-year-old woman developed multiple (>20) melanoma in-transit metastatic nodules on her right thigh (Figure 1A), as confirmed by histological examination, after removal, six months earlier, of a primary 2.2 mm thick ulcerated nodular melanoma on her right leg for which the initial sentinel lymph node biopsy was negative. After completion of FDG PET-CT to exclude distant metastasis and assessment of the extent of limb metastasis, the patient was referred to a specialized center to receive, isolated limb perfusion (ILP) of melphalan under hyperthermia, TNF-α and interferon γ. Superficial and deep pelvic (ilioinguinal) lymphadenectomy was simultane- ously performed and revealed two additional deep pelvic In vivo reflectance confocal microscopy: a useful non-invasive tool to assess the response to isolated limb perfusion for superficial pigmented melanoma in-transit metastatic disease. Report of a case Rastine Merat1, Wolf-Henning Boehncke1, Gürkan Kaya1 1 Division of Dermatology, University Hospital of Geneva, Switzerland Key words: reflectance confocal microscopy, isolated limb perfusion, metastatic melanoma Citation: Merat R, Boehncke W-H, Kaya G. In vivo reflectance confocal microscopy: a useful non-invasive tool to assess the response to isolated limb perfusion for superficial pigmented melanoma in-transit metastatic disease. Report of a case. Dermatol Pract Concept. 2017;7(2):10. DOI: https://doi.org/10.5826/dpc.0702a10 Received: September 10, 2016; Accepted: January 14, 2017; Published: April 30, 2017 Copyright: ©2017 Merat et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: None. Competing interests: The authors have no conflicts of interest to disclose. All authors have contributed significantly to this publication. Corresponding author: Rastine Merat, MD, PhD, Division of Dermatology, University Hospital of Geneva, Rue Gabrielle-Perret-Gentil 4, CH-1205 Geneva, Switzerland. Tel. +41.22.37.22.94.37; Fax. +41.22.372.94.77. Email: rastine.merat@hcuge.ch. Complete response can be difficult to assess after isolated limb perfusion (ILP) for metastatic in-transit melanoma, especially when numerous and unresectable post-necrotic persisting pigmented lesions oc- cur. These residual lesions are mainly seen in the more superficial and pigmented types of metastatic disease and correspond to the residual melanophage granuloma that persists after tumor tissues un- dergo complete necrosis. Reflectance confocal microscopy (RCM) is a non-invasive technique that allows the exploration of the superficial dermis. Here, we present the case of a patient in whom nu- merous post-ILP limb residual pigmented lesions were explored using combined RCM and histological examination of sample lesions and could be characterized as non-active. This approach allowed us to avoid additional excisions. ABSTRACT Figure 1. (A) In-transit metastatic nodules before ILP. (B) Persisting non-changing pigmented macules after ILP. The designated lesions (A, B) are the ones for which dermoscopy follow-up is shown in Figure 4. [Copyright: ©2017 Merat et al.] A B 48 Observation | Dermatol Pract Concept 2017;7(2):10 pigmented lesions (Figure 1B) in which a similar characteristic image was consistently seen. This approach allowed us to avoid additional excision. The patient has now been followed for more than a year without any clinical or radiological sign of relapse and most lesions have slowly resolved (Figure 4). Discussion Around 5-10% of patients with malignant melanoma develop lymphatic dissemination and in-transit metastasis. Even in the era of effective systemic therapies, isolated limb perfu- sion (ILP) with hyperthermia performed using various agents (melphalan, with addition of chemokines, i.e., TNF-α and/or other chemokines) is still a valuable therapeutic approach and is being used for patients who develop numerous limb lesions simultaneously and are therefore considered inoperable [1]. According to the literature, a 50% complete response rate can be obtained with ILP [2]. Nevertheless complete response can be difficult to assess if post-necrotic persisting pigmented lymph node metastases. One month after the end of this procedure, most in-transit lesions had undergone necrosis. Four months later, all lesions had healed and persisted as non-changing pigmented macules (Figure 1B) with no clini- cal or radiological (including FDG PET-CT) signs of relapse. In order to confirm that none of the residual pigmented macules was an active lesion, two of them were excised after being previously examined by RCM. Histological examina- tion showed a persisting melanophage granuloma (nodular melanosis) within areas of fibrosis in the upper dermis with no sign of tumor infiltration (Figure 2A and 2B). RCM imag- ing, performed using the Vivascope 1500 (VivaScope Systems, Munich, Germany) showed ill-defined aggregates of dermal bright cells, typical of melanophages with almost no visible nuclei. Sometimes a characteristic ring-shaped deposit was seen within the network of bright collagen fibers and bundles (Figure 3). Such deposits could be seen in many areas of the excised lesions and correlated with the histological images. We then performed RCM imaging on 12 other main persisting Figure 3. RCM mosaic from the upper dermis: ill-defined aggregates of dermal bright cells typical of melanophages and characteristic ring- shaped deposits within the network of collagen fibers. [Copyright: ©2017 Merat et al.] Figure 2. Histology (A) Persisting melanophage granuloma within areas of fibrosis in the upper dermis, original magnification x10. (B) Origi- nal magnification x20. Note the ring-shaped deposits as seen in RCM. [Copyright: ©2017 Merat et al.] A B Observation | Dermatol Pract Concept 2017;7(2):10 49 [3-6] where, in the case of superficial in- transit metastasis, post-necrotic residual melanophage granuloma (nodular mela- nosis) might be localized. Morphologic features of melanophages under in vivo RCM have been well characterized, allowing their distinction from mela- nocytes [7,8]. Here, using combined RCM and histological examination of two sample lesions, numerous unresect- able post-ILP limb residual pigmented lesions were considered by RCM to be non-active as confirmed by the subse- quent dermoscopy follow-up. Although in the case presented here there was not any doubt at follow-up regarding the absence of residual disease, considering that the deeper reticular dermis cannot lesions occur, especially if numerous and unresectable. These residual lesions occur mainly in the more superficial and pigmented types of metastatic dis- ease. As it was the case with our patient, these lesions can often correspond to the residual melanophage granuloma (nodular melanosis) that persists after tumor tissues undergo complete necrosis and can be difficult to distinguish from partial response and persisting in-tran- sit metastasis. RCM is a non-invasive technique that allows the exploration of the superficial dermis. Confocal- histopathological correlation has been shown to be possible for the visualiza- tion of some histologic features in the superficial dermis up to 300um deep Figure 4. Examples of dermoscopy follow-up of pigmented macules after ILP (A1, B1) and one year later (A2, B2). Note the gradual pigment resorption. [Copyright: ©2017 Merat et al.] be explored with RCM, biopsy remains mandatory in case of doubt during the subsequent needed close follow-up. References 1. Nieweg OE, Kroon BB. Isolated limb per- fusion with melphalan for melanoma. J Surg Oncol. 2014;109: 332-337. 2. Olofsson R, Mattsson J, Lindnér P. Long- term follow-up of 163 consecutive patients treated with isolated limb perfusion for in- transit metastases of malignant melanoma. Int J Hyperthermia. 2016;29:551-557. 3. Hofmann-Wellenhof R, Pellacani G, Malvehy J, Soyer HP. Reflectance Confo- cal Microscopy for Skin Diseases. 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Morphologic features of melanophages under in vivo reflectance confocal micros- copy. Arch Dermatol. 2010;146:492-498.