Dermatology: Practical and Conceptual Research | Dermatol Pract Concept 2017;7(4):2 3 DERMATOLOGY PRACTICAL & CONCEPTUAL www.derm101.com Cell junction protein armadillo repeat gene deleted in velo-cardio-facial syndrome is expressed in the skin and colocalizes with autoantibodies of patients affected by a new variant of endemic pemphigus foliaceus in Colombia Ana Maria Abreu-Velez1, Hong Yi2, Michael S. Howard1 1 Georgia Dermatopathology Associates, Atlanta, GA, USA 2 Robert P. Apkarian Integrated Electron Microscopy Core, Emory University Medical Center, Atlanta, GA, USA Key words: endemic pemphigus foliaceus, autoimmunity, cell junctions, ARCVF, skin Citation: Abreu-Velez AM, Yi H, Howard MS. Cell junction protein armadillo repeat gene deleted in velo-cardio-facial syndrome is expressed in the skin and colocalizes with autoantibodies of patients affected by a new variant of endemic pemphigus foliaceus in Colombia. Dermatol Pract Concept 2017;7(4):3-8. DOI: https://doi.org/10.5826/dpc.0704a02 Received: June 7, 2017; Accepted: July 31, 2017; Published: October 31, 2017 Copyright: ©2017 Abreu-Velez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Declaration of sources of funding: Our work was performed with funding from Georgia Dermatopathology Associates and Mineros SA, El Bagre, the Hospital Nuestra Señora del Carmen, El Bagre; and El Bagre Municipal Office, Colombia, South America. Competing interests: The authors have no conflicts of interest to disclose. All authors have contributed significantly to this publication. Corresponding author: Ana Maria Abreu-Velez, MD, PhD, Georgia Dermatopathology Associates, 1534 North Decatur Rd NE, Suite 206, Atlanta, GA 30307-1000, USA. Tel. (404) 371-0027; Fax: (404) 371-1900. Email: abreuvelez@yahoo.com. Background: We previously described a new variant of endemic pemphigus foliaceus in El Bagre, Colombia, South America (El Bagre-EPF, or pemphigus Abreu-Manu). El Bagre-EPF differs from other types of EPF clinically, epidemiologically, immunologically and in its target antigens. We reported the presence of patient autoantibodies colocalizing with armadillo repeat gene deleted in velo-cardio-facial syndrome (ARVCF), a catenin cell junction protein colocalizing with El Bagre-EPF autoantibodies in the heart and within pilosebaceous units along their neurovascular supply routes. Here we investigate the presence of ARVCF in skin and its possibility as a cutaneous El Bagre-EPF antigen. Methods: We used a case-control study, testing sera of 45 patients and 45 controls via direct and indirect immunofluorescence (DIF/IIF), confocal microscopy, immunoelectron microscopy and im- munoblotting for the presence of ARVCF and its relationship with El Bagre-EPF autoantibodies in the skin. We also immunoadsorbed samples with desmoglein 1 (Dsg1) ectodomain (El Bagre-EPF antigen) by incubating with the positive ARVCF samples from DIF and IIF. Results: ARVCF was expressed in all the samples from the cases and controls. Immunoadsorption with Dsg1 on positive ARVCF immunofluorescence DIF/IIF cases showed that the immune response was present against non-desmoglein 1 antigen(s). Overall, 40/45 patients showed colocalization of their autoantibodies with ARVCF in the epidermis; no controls from the endemic area displayed co- localization. Conclusions: We demonstrate that ARVCF is expressed in many areas of human skin, and colocalizes with the majority of El Bagre-EPF autoantibodies as a putative antigen. ABSTRACT 4 Research | Dermatol Pract Concept 2017;7(4):2 tures, and whether we could further confirm colocalization of El Bagre-EPF autoantibodies with ARVCF in the skin. Materials and Methods A human quality assurance review board approved the stud- ies at the Hospital Nuestra Señora del Carmen in El Bagre, and all participants provided signed consent. We tested 45 patients affected by El Bagre-EPF, and 45 controls from the endemic area matched by age, sex, demographics, comor- bidities, and work activities. All of the tests were performed in both cases and controls. The patients and controls were evaluated by hematoxylin and eosin (H&E) histology, DIF, IIF, confocal microscopy (CFM), indirect immunoelectron microscopy (IEM) immunoblotting (IB), by immunoprecipita- tion (IP) and by ELISA. Only patients meeting diagnostic cri- teria for El Bagre-EPF were included; specifically, they had to display clinical and epidemiologic features described for this disease; live in the endemic area; and have serum displaying intercellular staining (ICS) between epidermal keratinocytes and to the basement membrane zone (BMZ) of the skin via either DIF or IIF using fluorescein isothiocyanate (FITC) con- jugated monoclonal antibodies to human total IgG or IgG4, as described elsewhere [5-9]. Further, each patient had to be positive by IB for reactivity against Dsg1 [6], as well as for plakin molecules; each patient’s serum immunoprecipitated a Concanavalin A (Con A) affinity-purified bovine tryptic 45 kDa fragment of Dsg1 [14]; and each patient’s serum had to yield a positive result using an ELISA test when screening for autoantibodies to pemphigus foliaceus (PF) antigens [15]. DIF and IIF: We performed our DIF and IIF as previously described [8,9]. DIF: In brief, we incubated a 4 μm thickness frozen skin section using PBS with 0.1% Triton X-100 and 1% normal goat serum for five minutes for partial permeabilization, to detect cytoplasmic, nuclear, and membrane binding putative antigens, and for blocking non-specific staining. The slides were then washed with PBS [8,9]. The nuclei of the cells were counterstained with 4,6-diamidino-2-phenylindole (DAPI, Pierce; Rockford, IL, USA). We used antibodies to ARVCF, source guinea pig, Cat. no. GP155; Progen Bio- technik (Heidelberg, Germany). For its secondary, we used Alexa Fluor®555 goat-anti-guinea pig (Molecular Probes Life Technologies/ThermoFisher Scientific; Waltham, MA, USA). All samples were run with positive and negative controls. We classified our findings as negative (-), weakly positive (+), moderately positive (++) and strongly positive (+++). For IIF, our substrate tissue included human, bovine, rat and mouse skin. Written consents were obtained from all patients, and Institutional Review Board permission was obtained from the Hospital Nuestra Señora de El Carmen, in El Bagre, Colombia. Introduction Endemic pemphigus foliaceus (EPF) is an autoimmune blister- ing disease characterized by the presence of autoantibodies (primarily to desmoglein 1 [Dsg1]). On direct immunofluores- cence (DIF) and indirect immunofluorescence (IIF), the EPF autoantibodies display intercellular staining (ICS) between epidermal keratinocytes [1-3]. In Brazil, EPF is titled fogo sel- vagem (FS) and principally affects children and young adults. EPF has also been documented in rural areas of South and Central America (Argentina, Bolivia, El Salvador, Paraguay, Peru and Venezuela) [1-3]. One additional variant has been described in Tunisia, Africa [4]. We previously described a new variant of EPF in El Bagre, Colombia, South America (El Bagre-EPF, or pemphigus Abreu-Manu). El Bagre-EPF differs from other types of EPF because it affects predominantly 30- to70-year-old or older males, and a few peri/postmenopausal females [5-7]. The patients are primarily miners, who also work in agricultural activities. The patients have autoantibodies to dermal eccrine, sebaceous and Meibomian glands, and their neurovascular bundles; to cardiac molecules; to Pacinian receptors and other cutaneous neural receptors; and to tarsal muscles and to other structures [8-10]. For over two decades, we have fol- lowed the El Bagre-EPF patients [5-7,8-10]. The disease was originally thought to be a new focus of Brazilian EPF with some differences [19]; however, we later documented it as a new and unique disease, resembling Senear-Usher syndrome. We documented El Bagre EPF to have a unique autoimmune response due to environmental and genetic factors [10-14]. We also previously reported that one-third of the El Bagre- EPF patients present with a systemic form, as observed in systemic lupus erythematosus [10-15]. We previously reported the colocalization of El Bagre-EPF patient autoantibodies with armadillo repeat gene deleted in velo-cardio-facial syndrome (ARVCF) in cardiac tissue, as well as within pilosebaceous units along their neurovas- cular supply routes [9,11]. ARVCF is an adhesion molecule of the catenin-like protein family that is one of the mutated molecules in the disease called velo-cardio-facial syndrome (VCFS). VCFS, along with DiGeorge syndrome (DGS), and conotruncal anomaly face syndrome are part of the disease complex termed chromosomal microdeletions dissecting (CMD): del22q11 syndrome [11-13]. CMD is known to be associated with multiple autoimmune diseases, as well as some alterations in the immune system [11-13]. ARVCF also is related to the protein p120 subfamily of armadillo-related proteins, which includes p0071 and delta-catenin/NPRAP; these are distantly related plakophilins, and are involved in cell-cell adhesion [11-13]. In our current study, we investi- gated whether ARVCF was expressed in the skin, including the sebaceous glands and their neurovascular supply struc- Research | Dermatol Pract Concept 2017;7(4):2 5 IIF: Performed as the DIF, but utilizing skin from plastic surgeries as an antigen source. Colocalization of the patient’s autoantibodies with com- mercial antibodies utilizing confocal microscopy (CFM): Our CFM studies were performed as previously described [8,9]. In brief, we utilized standard 20X and 40X objective lenses; each photoframe included an area of approximately 440 x 330 μm. Images were obtained using EZ-1 image analysis software (Nikon, Tokyo, Japan). For colocalization experi- ments with serum autoantibodies, we used the previously described antibodies to ARVCF. Indirect immunoelectron microscopy (IEM): Our tech- nique was performed as described [8,9]. In brief, postembed- ding immunogold labeling was performed on samples on the subjects of the study. Mouse skin was used as an antigen; the tissue was fixed in 4% glutaraldehyde with 0.2% parafor- maldehyde, and embedded in Lowicryl® resin [8,9]. The tissue was then sectioned at a 70 nm thickness. The samples were blocked with a solution from Aurion™ (Electron Microcopy Sciences/EMS; Hatfield, PA, USA). Grids were then washed with PBS-BSAC (Aurion™, EMS). The primary antibodies were incubated, washed, and a secondary antibody solution, specifically 10 nm Gold-conjugated protein A PBS-BSAC (Aurion, EMS™) was applied [8,9]. The samples were then double-stained with uranyl acetate and lead citrate. The samples were observed under a Hitachi H7500 transmission electron microscope. Immunogold particle images displaying any pattern of positivity were then converted to TIF format. Immunoadsorption of skin Dsg1 1 autoantibodies: Patients with El Bagre-EPF have circulating autoantibod- ies directed against Dsg1 [5,6]. Based on the fact that the purified tryptic fraction of Dsg1 induces loss of adhesion in organ culture and in a neonatal mouse model, it has been proposed that these anti-Dsg1 antibodies play a pathogenic role in blister formation. To investigate if the ARVCF putative autoantibodies reactivity was the same as shown by Dsg1, we performed immunoadsorption experiments as previously described by incubating the tryptic ectodomain of Dsg1 with samples that tested positive for DIF and IIF [14]. We used affinity tryptic-purified antigen, obtained as described using the Con A column [14]. In brief, protein samples were released from bovine snout epidermis by means of trypsin digestion, and purified with the use of a Con A column. The eluted Dsg1 antigen was then incubated with El Bagre-EPF DIF and IIF slides that were positive previously in colocaliza- tion of ARVCF with the El Bagre-EPF serum. Statistical analysis: We used Fisher exact test to compare two nominal variables (e.g., positive and negative) of anti- body response. P < 0.05 with a 95% of confidence or more was considered statistically significant. We used the software GraphPad QuickCalcs (GraphPad Sofware Inc., La Jolla, CA, USA). Results A clinical picture of an exfoliative lesion of El Bagre-EPF is shown in Figure 1a. In Figure 1b, we show the corresponding histologic pattern. Our testing demonstrated positive staining for ARVCF in all layers of the epidermis, on the basement membrane zone and in upper areas of the subjacent dermis (including cell junctions and neurovascular bundles) by DIF, IIF, CFM, IB, and IEM. See Figure 1c, demonstrating one example of DIF staining with colocalization of the El Bagre- EPF autoantibodies and ARVCF. Overall, 40/45 patients affected by El Bagre-EPF dem- onstrated autoantibodies that colocalized perfectly with the ARVCF antibody via DIF. In the remaining five patients affected by El Bagre-EPF we observed the expression of ARVCF, but the autoantibodies did not colocalize with the ARVCF antibody. In Table 1 are shown the results of the polyclonal immune response using DIF and IIF, the strength of the stains and the colocalization with the El Bagre-EPF autoantibodies and the ARVCF reactivity. The CFM studies also demonstrated 100% colocalization of the patient autoantibodies and the commercial antibody to ARVCF (p < 0.01) (Figure 1c, d). None of the controls from the endemic area showed autoantibodies colocalizing to ARVCF via DIF, IIF, CMF, or IEM. However, the ARVCF molecule is ubiquitous in the skin. After the immunoadsorption with the pre-incubated Dsg1 antigen with the patient samples testing positive for colocalization with ARVCF, the immunoreactivity to ARCVF persisted. Our IEM data (Figure 1e, f) demonstrated positive stain- ing for patient autoantibodies in skin cell junctions. The IB studies confirmed multiple antigens detected by patient sera, that also perfectly colocalized with the Progen antibody to ARVCF (data not shown). Discussion To our knowledge, we present evidence for the first time in the medical literature that ARVCF is expressed in the skin via diverse methods. We also demonstrated that El Bagre-EPF autoantibodies colocalized with the commercial antibody to ARVCF from Progen in the skin of 40/45 patients. We further demonstrated that after immunoadsorption with an ectodomain of Dsg1 the reactivity remained, demonstrating that the El Bagre-EPF patient’s autoantibodies are directed to a non-Dsg1 antigen. We noted that 5/45 El Bagre-EPF patients did not display autoantibodies against ARVCF. El Bagre EPF patients pres- ent a diverse spectrum of clinical lesions (hyperpigmented, prurigoid, generalized, blistering, etc.) [1,3,5]. The patients may also present in an acute, relapsing or chronic stage of https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&uact=8&ved=0ahUKEwjkxrSelOXSAhUFSCYKHYPMDSMQFgg4MAE&url=http%3A%2F%2Fgraphpad.com%2Fquickcalcs%2Fcontingency1%2F&usg=AFQjCNFmcQtFUoz8qKLz92JezpJ4WqbViQ https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&uact=8&sqi=2&ved=0ahUKEwjZw4OC6uXSAhXLRyYKHUj3ACgQFggjMAE&url=https%3A%2F%2Fen.wikipedia.org%2Fwiki%2FImmunoadsorption&usg=AFQjCNEU_H_TlpiRO8yFpaaXwntlqaH4OQ&bvm=bv.150120842,d.eWE 6 Research | Dermatol Pract Concept 2017;7(4):2 metals and other metalloids [5] that can compete with cal- cium. El Bagre soils are extremely acidic, contain very scarce organic matter and are thus scarce in calcium, phosphates, magnesium (Mg++), and potassium. Phosphates are constitu- ents of ATP, DNA, RNA, and the phospholipids, which form all cell membranes where the cells junctions are situated. The existence or absence of a given metal/metalloid is crucial to the conformation or activity of most proteins. Thus, the metal/metalloid ions in the environment could compete with physiologic ions (e.g., Ca++), altering the conformation of relevant molecules and generating antigenicity. ARVCF (A.K.A. catenin delta 1) binds sulfate (S04) as well as magnesium (Mg++ are their ligands. DP also has an S04 ligand and to phosphoserine is classified as UniProtKB/ disease. Thus, each case may present a range of autoantigens and immune responses, depending of the clinical phase of the disease. We also showed that the immune response is polyclonal in patients affected by El Bagre-EPF, as previously described [8,9]. We speculate that disruption of epidermal cells in El Bagre-EPF could induce epitope spreading, or expose new molecules that could then become antigenic since like Dsg1, ARVCF represents a cell junction protein. ARVCF is a protein with three cellular locations: in the plasma membrane, the cytoplasm and the nucleus [13-16]. We also hypothesize that ARVC becomes antigenic because this molecule is calcium (Ca++) dependent (similar to DSg1); further, that the El Bagre soils are polluted by mercury, Figure 1. (a) An El Bagre EPF clinical blis- ter (black arrow). (b) H&E staining of one of multiple patterns seen in El Bagre EPF. In this case, note the loss of the upper lay- ers of the epidermis (black arrow), and a dilated dermal blood vessel (blue arrow) with dermal edema and inflammation. (c) DIF of the skin from a patient affected by El Bagre-EPF, showing positive double stain- ing in the epidermis (in orange stain, as re- sult of the patient’s autoantibodies in green and the ARVCF in red) (+++) (black arrow), basement membrane zone (greenish stain) (+++) (white arrow) and a dermal neurovas- cular bundle (yellowish stain) (+++) (blue arrow) using FITC conjugated anti-human IgG (green staining) and Alexa 555 con- jugated ARVCF (red staining). The stain- ing observed represents overlap staining of different strength from yellowish-orange to green (400X). (d) Confocal microscopy, with multiple channels of fluorescence uti- lized. In the presented case we used a FITC channel (green peaks) (Excitation/Emission (nm):495/519), a DAPI channel (blue peaks) (Excitation/Emission (nm):360⁄460), and an Alexa Fluor®555 channel (red peaks) (Exci- tation /Emission (nm):555/568). The graphic shows the colocalization of the peaks of the immunofluorescence of the patient’s anti- bodies (green peaks; white arrow) with the ARVCF antibody (red peaks; white arrow). Both green and red are aligned, demonstrat- ing colocalization. The blue peaks represent DAPI (nuclear counterstaining). (e) and (f) IEM photographs of patient skin, show- ing ARVCF antibodies labelled with 10 nm Gold-conjugated protein A antibodies (tiny black dots). In (e), the antibodies are located in the epidermal cells junctions (black arrow), at the cutaneous basement membrane zone (black dots; red arrow) and in the upper papillary dermis (tiny black dots; blue arrow) (100kV). (f) Positive IEM staining in the upper epidermal keratinocytes cells junction showing ARVCF antibody gold labeled grouped in some areas (tiny black dots; red arrows) (100kV). [Copyright: ©2017 Abreu Velez et al.] Research | Dermatol Pract Concept 2017;7(4):2 7 respective sequence is available to researchers to investigate if ARVCF may be a putative antigen in other autoimmune blistering diseases. More than 9% of the protein sequences provided by UniProtKB are derived from translation of the coding sequences. Acknowledgement We wish to thank Jonathan S. Jones, HT (ASCP) at Georgia Dermatopathology Associates for excellent technical assis- tance. References 1. Diaz LA, Sampaio SA, Rivitti EA, et al. Endemic pemphigus folia- ceus (fogo selvagem). I. Clinical features and immunopathology. J Am Acad Dermatol. 1989;20(4):657-659. 2. Ortega-Loayza AG, Ramos W, Gutierrez EL, et al. Endemic pem- phigus foliaceus in the Peruvian Amazon. Clin Exp Dermatol. 2013;38(6):594-600. 3. Castro RM, Proença NG. Similarities and differences between Brazilian wild fire and pemphigus foliaceus Cazenave. Hautarzt. 1982;33(11):574-577. 4. Morini JP, Jomaa B, Gorgi Y, et al. Pemphigus foliaceus in young women. An endemic focus in the Sousse area of Tunisia. Arch Dermatol. 1993;129(1):69-73. 5. Abreu-Velez AM, Hashimoto T, Bollag W, et al. A unique form of endemic pemphigus in Northern Colombia. J Am Acad Dermatol. 2003(4);49:599-608. 6. Abreu-Velez AM, Beutner EH, Montoya F, Hashimoto T. Analyses of autoantigens in a new form of endemic pemphigus foliaceus in Colombia. J Am Acad Dermatol 2003;49(4):609-614. 7. Hisamatsu Y, Abreu-Velez AM, Amagai M, Ogawa MM, Kan- zaki T, Hashimoto T. Comparative study of autoantigen profile between Colombian and Brazilian types of endemic pemphigus foliaceus by various biochemical and molecular biological tech- niques. J Dermatol Sci. 2003.32(1):33-41. 8. Howard MS, Abreu-Velez AM. Broad histopathologic patterns of non-glabrous skin and glabrous skin from patients with a new variant of endemic pemphigus foliaceus (Part 1). J Cutan Pathol. 2010;37(2):222-230. Swiss-Prot (O00192), and is a member of the catenin family, which contain an armadillo/beta-catenin-like repeat sequence [11-13]. ARVCF is a Ca++ dependent cell-cell adhesion mol- ecule, acting via plasma membranes. The catenin family plays an important role in the formation of adherens junction complexes, which are thought to facilitate communication between the inner and outer environments of cells [11-13]. The data suggest that ARVCF is a putative El Bagre-EPF anti- gen. Our data indicates that ARVCF is a putative non-Dsg1 antigen in El Bagre-EPF patients. The p120 protein family includes p120-catenin, δ-catenin, p0071, and ARVCF [10]. ARVCF associates with p68, hnRNP H2, SFF1, zonula occludens ZO-1 and ZO-2 (in the last two, binding to PDZ-domain proteins) [11-13]. All three diseases belonging to the CMD syndrome are associated with impaired function of T cells due to abnormal development of the thymus gland, abnormalities of T-cell clonality, and/or in B lymphocytic function [11-13,16]. We believe there is an abnormality in the immune response in El Bagre-EPF patients, which we are currently investigating. Pemphigus Abreu-Manu differs from previously described forms of EPF. Indeed, El Bagre-EPF is a chronic inflammatory disease that has protean manifestations, including a form fruste (localized to the skin and resembling Senear-Usher syndrome, and with a unique autoimmune response due to environmental and genetic factors) with photosensitivity; and a systemic form [8,9]. Patients affected by this disease may present with relapsing episodes; the systemic form affects multiple organs with a less favorable prognosis [5,6,12]. In conclusion, we demonstrate the expression of the pro- tein ARCVF in multiple areas of the skin. We prove colocal- izing or this protein with the majority of the El-Bagre-EPF autoantibodies, and that the reactivity is directed against a non-Dsg1 antigen. We aim in our future research to establish the precise pathologic role of ARVCF in El Bagre-EPF patients and in the skin. The ARVCF UniProtKB/Swiss-Prot (000192) TABLE 1. DIF and IIF autoantibody staining in the skin and colocalization with ARVCF DIF IIF El Bagre-EPF autoantibodies Number of positive cases Strength of staining Colocalization with ARVCF Number of positive cases Strength of staining Colocalization with ARVCF IgG 40/45 (+++) 100% 39/45 (+++) 100% Fibrinogen 39/45 (+++) 100% 38/45 (++) 100% IgM 38/45 (+++) 100% 38/45 (+++) 100% Albumin 38/45 (+++) 100% 38/45 (++) 100% Complement/C3c 35/45 (+++) 100% 35/45 (++) 100% Complement/C1q 35/45 (++) 100% 35/45 (++) 100% IgA 15/45 (++) 100% 15/45 (++) 100% IgD 16/45 (++) 100% 16/45 (++) 100% IgE 7/45 (++) 100% 7/45 (++) 100% 8 Research | Dermatol Pract Concept 2017;7(4):2 proteins and cell-cell adhesion regulate plasma membrane and nuclear localization of ARVCF. Mol Biol Cell. 2004;15(12):5503- 5515. 14. Abreu-Velez AM, Javier Patiño P, Montoya F, Bollag WB. The tryp- tic cleavage product of the mature form of the bovine desmoglein 1 ectodomain is one of the antigen moieties immunoprecipitated by all sera from symptomatic patients affected by a new variant of endemic pemphigus. Eur J Dermatol. 2003;13(4):359-366. 15. Abréu-Vélez AM, Yepes MM, Patiño PJ, Bollag WB, Montoya F Sr. A cost-effective, sensitive and specific enzyme linked immu- nosorbent assay useful for detecting a heterogeneous antibody population in sera from people suffering a new variant of endemic pemphigus. Arch Dermatol Res. 2004; 295(10):434-441. 16. McLean-Tooke A, Spickett GP, Gennery AR. Immunodeficiency and autoimmunity in 22q11.2 deletion syndrome. Scand J Im- munol. 2007;66(1):1-7. 9. Abreu-Velez AM, Zhe J, Howard MS, Dudley SC. Cardiac au- toantibodies from patients affected by a new variant of endemic pemphigus foliaceus in Colombia, South America. J Clin Immu- nol. 2011;31(6):985-997. 10. Abreu-Velez AM, Howard MS, Hashimoto T, Grossniklaus HE. Human eyelid meibomian glands and tarsal muscle are recognized by autoantibodies from patients affected by a new variant of en- demic pemphigus foliaceus in El-Bagre, Colombia, South America. J Am Acad Dermatol. 2010;62(3):437-447. 11. Hatzfeld M. The p120 family of cell adhesion molecules. Eur J Cell Biol. 2005;84(2-3):205-214. 12. Mariner DJ, Sirotkin H, Daniel JM, et al. Production and char- acterization of monoclonal antibodies to ARVCF. Hybridoma. 1999;18(4): 349-399. 13. Kausalya PJ, Phua DC, Hunziker W. Association of ARVCF with zonula occludens (ZO)-1 and ZO-2: binding to PDZ-domain