Dermatology: Practical and Conceptual Letter | Dermatol Pract Concept 2018;8(4):8 295 DERMATOLOGY PRACTICAL & CONCEPTUAL www.derm101.com Introduction Skin tags are benign tumors and are very common in the general population. Skin friction can cause skin rupture and can represent an access for pathogens such as HPV. This mechanism could explain a more frequent localization of skin tags in anatomical sites such as the neck or armpits. Mechanical friction and possible presence of HPV DNA were suggested as significant cofactors in the pathogenesis of soft fibromas [1]. Dianzani et  al [1] highlighted the presence of HPV type 6 and 11 DNA in 88% of the 49 soft fibromas. Other studies reported a positive HPV DNA in 71.4% of biopsies from soft fibromas, whereas HPV detection rate was 13.3% in normal skin samples. Thereafter, Al-Shaiji and Al-Buai- nian [3] reported that 30 individuals with soft fibromas showed positivity for HPV 6 and 11 DNA in 77% of cases. Other studies reported that 48.6% of 37 skin fibrous speci- mens were positive for HPV 6 and 11 DNA but they did not report any relationship between HPV and soft fibromas of patients. These conflicting reports might reflect differences in material source, sample size, geographical distribution, and methods sensitivity. Thus, the possible association between fibromas and HPV was revised in the present work in the light of new experimental procedures [2]. Case Presentation Twenty patients were enrolled at the dermatological clinic of the “Campus Biomedico” in Rome. The study was approved by Institutional Review Board and all patients gave informed consent to participate in this study. Anamnestic and clinical- histological data, as well as information about risk factors such as a smoking habit and body mass index (BMI), were collected in a specially designed database and are illustrated in Table 1. The results revealed detection of cutaneous HPV and mucosal HPV in all 20 of the tested subjects. Excisional biopsies were performed in all patients with skin tags, completely removing the lesion. Samples were stored at +4°C in ThinPrep(r) and utilized for DNA and RNA extraction by commercial kits [2]. Human papillomavirus in skin tags: a case series Caterina Dianzani1, Francesca Paolini2, Claudio Conforti1,3, Maria Silvestre1, Francesca Flagiello1, Aldo Venuti2 1 Department of Plastic, Reconstructive and Cosmetic Surgery, Campus Bio-Medico University Hospital, Rome, Italy 2 HPV Unit, UOSD Tumor Immunology and Immunotherapy, Regina Elena National Cancer Institute, Rome, Italy 3 Dermatology Clinic, Hospital Maggiore, Piazza dell’Ospitale 1, Trieste, Italy Key words: human papillomavirus, skin tags, fibromas, virology, HPV, skin cancer Citation: Dianzani C, Paolini F, Conforti C, Silvestre M, Flagiello F, Venuti A. Human papillomavirus in skin tags: a case series. Dermatol Pract Concept. 2018;8(4):295-296. DOI: https://doi.org/10.5826/dpc.0804a08 Received: March 8, 2018; Accepted: April 6, 2018; Published: October 31, 2018 Copyright: ©2018 Dianzani et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: None. Competing interests: The authors have no conflicts of interest to disclose. All authors have contributed significantly to this publication. Corresponding author: Claudio Conforti, MD, Dermatology Clinic, Hospital Maggiore, Piazza dell’Ospitale 1, Trieste, Italy. Email: dermatologiaconforti@gmail.com 296 Letter | Dermatol Pract Concept 2018;8(4):8 References 1. Dianzani C, Calvieri S, Pierangeli A, Imperi M, Bucci M, Degener A. M The detection of human papillomavirus DNA in skin tags. Br J Dermatol. 1998;138:(4):649-651. 2. Dianzani C, Paolini F, Conforti C, Riva E, Beninati E, Venuti A. Human papillomavirus expression in immunocompetent pa- tients with actinic keratosis: a case series. J Am Acad Derma- tol. 2017;77(4):770-772. 3. Al Shajii A, Al Buainain H. Skin tag in relation with human papil- loma virus. The Gulf Journal of Dermatology. 2005;12:31-33. The presence of HPV DNA was detected by the poly- merase chain reaction (PCR) and rolling circle amplification (RCA). Nested PCR with CP65/CP70 outer-CP66/CP69 inner, and MY09/MY11 outer-GP5+/GP6+inner primers was per- formed for the detection of cutaneous HPV and mucosal HPV, respectively. Amplified products were subjected to direct sequencing in an automated apparatus. Same primers were utilized for reverse-transcriptase and real-time PCR on extracted RNA. These techniques are standardized proce- dures that were already utilized in a previous work [2]. HPV DNA analyses showed presence of HPV in 50% of patients. The HPV genotype distribution is showed in Figure 1. All HPVs were episomal, as indicated by RCA, whereas HPV RNA transcripts were undetectable, at least with the sensitivity of the test. Conclusion Data from our study confirms the presence of HPV DNA in skin tags, suggesting the possible role of these viruses in their development. On the other hand, the lack of viral transcripts could indicate a latent infection by HPV. Furthermore, our data have highlighted the presence of high risk HPVs (types 18 and 16). Given that we examined only 20 patients, we can only hypothesize that skin tags may represent a reservoir of carcinogenic HPVs. TABLE 1. Clinical and anamnestic data of patients Patient Sex Age (Years) Anatomical Site Mucosal HPV Cutaneous HPV 1 M 77 Back 2 F 72 Back 3 F 66 Right hip 4 F 77 Left ear 18 5 F 89 Neck 6 F 43 Armpit 18 7 F 60 Back 6 8 M 64 Back 18 9 F 65 Neck 10 M 40 Neck 6 11 M 71 Armpit 93 12 M 57 Armpit 5 13 F 59 Armpit 14 F 41 Armpit 15 F 55 Armpit 16 16 F 45 Neck 17 M 20 Back 18 F 58 Back 16 19 F 45 Armpit 20 M 24 Neck 174 Figure 1. HPV genotypes. Percentages refer to the total number of HPVs plus samples. [Copyright: ©2018 Dianzani et al.]