Dermatology: Practical and Conceptual 28 Research | Dermatol Pract Concept 2019;9(1):8 Dermatology Practical & Conceptual Introduction The rate of surgical site infection (SSI) for clean dermatologi- cal surgery is usually less than 3% [1-3], which is significantly lower than the 5% acceptable SSI rate quoted by many authorities [4,5]. The incidence of SSI is influenced by body site, and certain anatomical sites are at much higher risk than the acceptable rate [6-9], with lower limb surgery most con- sistently complicated by infection [3,10-16]. In tropical North Queensland, the infection rates following excisions from the Effect of a Single Preoperative Dose of Oral Antibiotic to Reduce the Incidence of Surgical Site Infection Following Below-Knee Dermatological Flap and Graft Repair Helena Rosengren1, Clare F. Heal2, Petra G. Buettner3 1 School of Medicine, James Cook University, Townsville, Queensland, Australia; Skin Cancer College of Australasia, Brisbane, Queensland, Australia; Skin Repair Skin Cancer Clinic, Townsville, Queensland, Australia 2 School of Medicine, James Cook University, Mackay, Queensland, Australia 3 Centre for Chronic Disease Prevention, James Cook University, Cairns, Queensland, Australia Key words: antibiotic prophylaxis, surgical site infection, dermatological surgical wound infection, operative surgical procedures, grafts Citation: Rosengren H, Heal CF, Buettner PG. Effect of a single preoperative dose of oral antibiotic to reduce the incidence of surgical site infection following below-knee dermatological flap and graft repair Dermatol Pract Concept. 2019;9(1):28-35. DOI: https://doi.org/10.5826/ dpc.0901a08 Published: January 31, 2019 Copyright: ©2019 Rosengren et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: None. Competing interests: The authors have no conflicts of interest to disclose. Authorship: All authors have contributed significantly to this publication. Corresponding author: Dr. Clare Heal, James Cook University, Mackay, Queensland, Australia. Email: clare.heal@jcu.edu.au Background: Surgical site infection (SSI) rates for below-knee dermatological surgery are unaccept- ably high, particularly following complex flap and graft closures. The role of antibiotic prophylaxis for these surgical cases is uncertain. Objective: To determine whether SSI following complex dermatological closures on the leg could be reduced by antibiotic prophylaxis administered as a single oral preoperative dose. Methods: A total of 115 participants were randomized to 2 g of oral cephalexin or placebo 40-60 minutes prior to surgical incision in a prospective, randomized, double-blind, placebo-controlled trial at a primary care skin cancer clinic in North Queensland, Australia. Results: Overall 17/55 (30.9%) controls and 14/55 (25.5%) intervention participants developed in- fection (P = 0.525). There was no difference between the study groups in adverse symptoms that could be attributed to high-dose antibiotic administration (P = 1). Conclusion: A single oral 2-g dose of cephalexin given before complex below-knee dermatological closure did not reduce SSI. ABSTRACT Research | Dermatol Pract Concept 2019;9(1):8 29 Despite the importance of this topic, few randomized controlled trials (RCTs) have been published on the use of oral antibiotic prophylaxis in dermatological surgery [17]. The aim of our random- ized, double-blind, placebo-controlled trial was to ascertain the effect of a single preoperative oral prophylactic antibiotic dose on SSI following com- plex below-knee dermatological surgery. Methods This randomized, double-blind, pla- cebo-controlled trial recruited consecu- tive eligible patients booked for flap or graft closure following skin cancer excision below the knee at a primary care skin cancer clinic in Townsville, North Queensland, Australia, between January 2014 and February 2016. Each individual patient gave signed informed consent and was allowed to participate in the trial only once. Specific exclusion criteria (Figure 1) were age under 18, taking any anti- biotic within 48 hours of the surgery, suspected SSI at the time of surgery, allergy to the protocol suture, dressing materials, cephalosporins or penicillin, intellectual or mental impairment, and previous participation in this study. If histology confirmed the need for a wider excision or participants received antibi- otics for another SSI during the 30-day study period, participants were to be withdrawn from the study. lower limb have ranged from 14.75% to 18.18% [13,16]. Below-knee surgery has been shown to have an even higher infection rate [13,16,17]. The reasons for this are unclear, but reduced perfu- sion pressure in the distal limbs [18], higher tension closures [19], as well as the frequent necessity for complex graft/ flap surgery are postulated reasons. More complex skin closures, such as flap and graft procedures, are known also to be independently associated with significantly higher risk of SSI [20]. Large observational prospective studies have shown that flap repair increases the likelihood of infection by 2 to 15 times compared with simple elliptical closure [3,18,20,21]. Graft repair has also been linked to much higher infec- tion rates [3,18,20,21]. Established SSI may require mul- tiple medical visits, can result in poorer cosmetic outcome and significant bac- teremic complications [8,17], and also requires several days of treatment with antibiotics. Antibiotic treatment may be associated with unpleasant side effects, allergy, and the development of antibiotic resistance [8]. As a result of indiscriminate antibiotic prescribing, antibiotic resistance is increasing at a dramatic rate, causing significant mor- bidity and mortality globally [22,23]. A single, high-dose preoperative oral pro- phylactic antibiotic has been proposed for anatomical sites and dermatological procedures at high risk of SSI [8]. It is suggested that administration of such a single prophylactic dose may be less likely to result in antibiotic resistance than a longer-term course prescribed for established infection, with a resulting reduction in the quantity of antibiotics prescribed overall [17,19,24]. If antibiotic prophylaxis is to be effective, antibiotics should optimally be in the bloodstream and at the opera- tive site at the time of incision [25,26]. The administration of antibiotics within 2 hours prior to incision is associated with the lowest risk of SSI [26]. Figure 1. Inclusion and exclusion criteria for study. [Copyright: ©2019 Rosengren et al.] Capsules containing either 2 g of cephalexin or placebo were given 30 minutes prior to the excision appoint- ment time, ensuring that skin incision would be within 60 minutes of inges- tion of capsules. At the time of surgery baseline demographic data, pertinent medical and drug history, defect size, time from intervention to incision, and closure technique were documented. Histology was documented once it had been reported. Intra- and Postoperative Protocol If the wound required deep dermal absorbable sutures, Monosyn (B. Braun, Sydney, Australia) was used. All defects were closed superficially with nylon sutures (Dynek Pty Ltd., Hendon, South Australia). Following wound closure, Melolin (Smith and Nephew Medical Ltd, Hull, UK) and Fixomull (BSN Med- ical, Luxenburg) was applied. Standardized verbal and written postoperative wound care instructions were given to all participants, stressing the importance of applying no topi- cal creams, ointments, or antiseptics to the wound for the month after surgery. All participants were reviewed 7 days postoperatively for wound inspection and redressing. Information related to the use of postoperative oral analge- sia (strongest analgesic used) was also recorded at this time. Early review at the study practice was encouraged in the event of discomfort, erythema, swelling, or discharge associated with the wound. 30 Research | Dermatol Pract Concept 2019;9(1):8 t test, chi-square test, and Fisher exact test. Intervention and control group dif- ferences at baseline as well as treatment modality differences were assessed using unpaired t test, Mann-Whitney test, chi- square test, and Fisher exact test. All data were analyzed using inten- tion-to-treat analysis. Incidence of SSI was compared between intervention and control groups using chi-square test. The difference in infection rate was calculated and presented with 95% con- fidence interval (95% CI). The number needed to treat for benefit was calcu- lated with 95% CI [29]. A sensitivity analysis was conducted including all 115 patients who were originally ran- domized by assuming various outcome scenarios for the 5 patients who were lost to follow-up. Logistic regression models were applied to assess poten- tial confounding effects of all baseline characteristics on difference in incidence of infection between intervention and control groups. Chi-square and Fisher exact tests were used to compare postoperative analgesia requirements, adverse effects, and treatment of occurring infections between intervention and control groups. Analysis was conducted using Sta- taIC12 (StataCorp LP, Lakeway Drive, College Station, TX, USA). A P value less than 0.05 was considered statistically significant. capsules filled with either antibiotic (intervention) or placebo (control) were transferred to screw-top containers numbered according to the randomiza- tion sequence and sent to the recruit- ing practice in batches. Clinic staff and participants remained blind to group allocation during the trial. Sample Size A small prestudy observational trial at the practice had shown approximately 25% infection rates for flap and graft closures below the knee. Our hypothesis was that antibiotic prophylaxis would reduce the infection rate to 5%. Forty- four patients were required in each study group to show this with statisti- cal confidence (power in excess of 80%; significance level 0.05). We planned to recruit a minimum of 110 participants (55 intervention and 55 placebo), allow- ing for a 25% drop out. Statistical Analysis and Presentation Statistical analysis and result prepara- tion followed the CONSORT guidelines [28]. Numerical data were described using mean and standard deviation when symmetrically distributed and median and interquartile range when skewed. Categorical data were presented using absolute and relative frequencies. Eligible nonparticipants were com- pared with participants using unpaired Sutures were removed 14 days after surgery. If at the time of suture removal the wound had not fully healed, it was dressed again and regularly reviewed until it had completely epithelialized. Clinical Outcome The wound was assessed at the time of suture removal (14 days postopera- tively) by 1 of the 6 treating doctors. An adapted version of the 1988 Centers of Disease Control and Prevention of National Nosocomial Infections Surveil- lance System definition for dermato- logical surgical site infection was used [27]. Standardized criteria for surgical site skin infection included occurrence within 30 days of surgery and puru- lent discharge, erythema more than 1 cm from wound edges, OR presence of localized swelling, heat, pain, or tender- ness (Figure 2). If SSI was suspected, a swab was taken for microscopy, culture, and sen- sitivity and a 5-day course of cephalexin (500 mg 4 times a day) was prescribed pending swab results. Each participant was phoned by the study nurse 1 month after surgery to ensure no SSI was inad- vertently missed. Randomization and Blinding The randomization sequence was gen- erated electronically using IBM SPSS Statistics (V 22.0, IBM Corp., Armonk, NY, USA) by author and statistician (P.G.B.) and emailed to a compound- ing pharmacy where batches of generic gel capsules had been filled with either cephalexin or placebo (microcrystal- line cellulose and calcium carbonate powder). Four identical-looking gel Figure 3. CONSORT Flow Diagram for Trial. [Copyright: ©2019 Rosengren et al.] Figure 2. Criteria for surgical site infec- tion in dermatological surgery. [Copyright: ©2019 Rosengren et al.] Research | Dermatol Pract Concept 2019;9(1):8 31 during the study follow-up period. Three participants (2 controls, 1 intervention) were lost to follow-up despite repeated attempts at phone contact. There were no demonstrable differences between the characteristics of the 5 participants who did not complete the study and the 110 who did. Surgical Site Infection The main analysis based on available cases at follow-up (Table 3) showed 17 (30.9%; n = 55) SSIs for control and 14 (25.5%; n = 55) for intervention partici- pants (P = 0.525). A sensitivity analysis including all 115 initially randomized patients did not alter this result, regard- less of whether the patients who were lost to follow-up were assumed to have had an infection or not to have had an infection (Table 3). Logistic regression analyses to assess effects of possible confounding variables did not alter the main result of the trial. Only 1 (interven- tion) of 8 split thickness graft closures developed SSI. All other cases of SSI occurred in flap closures. The difference in infection rate between the control and intervention groups was 5.4% (95% CI: 11.4%- 22.2%). Nineteen patients needed to be treated (number needed to benefit) to prevent an infection. The power to detect the 5.4% difference in infection rates between intervention and con- trol groups was 9.6%. A sample size of 1,089 participants in each group would have been needed to reach a power of 80%. Secondary Outcome Measures There was no difference in analgesia requirement between study groups, with the majority of participants (81.8% of controls; 78.2% of intervention group) taking no postoperative analgesia (Table 4). One patient in the intervention group reported nausea in the follow-up period. No other symptoms that might have been attributable to the intervention were recorded in either study group. Of the 115 participants, 57 were randomized to administration of pla- cebo (control group) and 58 to admin- istration of cephalexin (intervention group). The 2 study groups were com- parable at baseline for all variables mea- sured (age, sex, diabetes, smoking status, anticoagulant or immunosuppressive medication, anatomical site and histol- ogy of tumor, defect size, and repair technique) (Table 2). Defect closure was with flap repair for 106 (52 control, 55 intervention) participants and graft repair for 8 (5 control, 3 intervention) participants. The remaining participant had an elliptical closure. Graft repair in all cases was with split thickness skin taken from the area surrounding the defect. This method of graft closure is referred to as a halo graft [30]. Two participants (both intervention) violated protocol and were withdrawn from the study, one because the treating doctor opted to close the defect with an ellipse and the other because of needing antibiotic treatment for an unrelated SSI Results Sixteen of 152 consecutive patients requiring flap or graft closures below the knee were ineligible for this study because of penicillin or cephalosporin allergy (n = 9), taking antibiotics for unrelated reasons at the time of surgery (n = 4), and previous participation in this study (n = 3). Twenty-one eligible patients declined to participate. The most common rea- sons given for nonparticipation were not wanting to take unnecessary tablets (n = 12) and fear of possible diarrhea (n = 3). Other reasons given (n = 6) were being too old, fear of allergy, and being unwell on the day of the procedure. With the exception of site tumor, there was no difference between eligible nonparticipants and participants (Table 1). Nonparticipants were more likely to have the neoplasm on the anterior leg whereas participants were more likely to have it on the calf (P = 0.030) (Table 1). TABLE 1. Comparison of Nonparticipants Who Fulfilled In- clusion Criteria With Those Participating in the Study Characteristic Nonparticipants (n = 21) Participants (n = 115) P Value Mean age (SD), years 71.9 (12.4) 69.4 (10.8) 0.336 Male (%) 10 (47.6%) 73 (63.5%) 0.171 Body site of lesion (%) Foot Ankle Anterior leg Calf 1 (4.8%) 1 (4.8%) 15 (71.4%) 4 (19.0%) 8 (7.0%) 6 (5.2%) 44 (38.3%) 57 (49.6%) 0.030 Histologya (%) BCC SCC Keratoacanthoma Melanoma 6 (30.0%) 11 (55.0%) 2 (10.0%) 1 (5.0%) 54 (47.0%) 48 (41.7%) 7 (6.1%) 6 (5.2%) 0.059 Active smokera (%) 0 2 (1.7%) 1.0 Type 2 diabetes mellitusa (%) 1 (5.0%) 13 (11.3%) 0.693 User of anticoagulant medicationa (%) 0 27 (23.5%) 0.065 User of immunosuppressive medicationa (%) 0 1 (0.9%) 1.0 BCC = basal cell carcinoma; SCC = squamous cell carcinoma; SD = standard deviation. a Missing information for 1 nonparticipant. 32 Research | Dermatol Pract Concept 2019;9(1):8 Swab Results for SSI Cases All patients with clinical SSI were treated with antibiotics, the majority (16/17 controls and 12/14 interven- tion participants) receiving cephalexin (Table 4). Doctors forgot to take swabs for microscopy, culture, and sensitivity in 5 participants presenting to the study clinic with suspected SSI. A further 5 study participants presented to clini- cians elsewhere (hospital in 2 cases, own family doctor in 3 cases) with suspected SSI and were treated with antibiotics without first taking swabs. Of the 10 participants (5 control, 5 intervention) with suspected SSI not confirmed on microscopy, 8 were treated with cephalexin and 1 with dicloxacillin. In the final case, we were unable to ascertain what antibiotic had been prescribed. Clinical SSI settled in all 10 cases without the need for further intervention. Of the 21 swabs taken, 12 (8 control, 4 intervention) grew Staphylococcus aureus sensitive to cephalexin, 1 (inter- vention) produced Stenotrophomonas maltophilia sensitive to cephalexin, and a further 4 (2 control, 2 intervention) developed no organism. Clinical SSI in all of these 17 swabbed cases responded fully to cephalexin which had been pre- scribed. TABLE 2. Baseline Comparison of Control Group (n = 57) With Intervention Group (n = 58) Characteristic Control (n = 57) Intervention (n = 58) P Value Mean age (SD), years 69.4 (11.6) 69.4 (10.1) 0.982 Male (%) 34 (59.6%) 39 (67.2%) 0.398 Body site of lesion (%) Foot Ankle Anterior leg Calf 3 (5.3%) 2 (3.5%) 21 (36.8%) 31 (54.4%) 5 (8.6%) 4 (6.9%) 23 (39.7%) 26 (44.8%) 0.633 Histology (%) BCC SCC Keratoacanthoma Melanoma 30 (52.6%) 18 (33.4%) 5 (8.8%) 3 (5.3%) 24 (41.4%) 29 (50%) 2 (3.4%) 3 (5.2%) 0.412 Active smoker (%) 1 (1.8%) 1 (1.7%) 1.0 Type 2 diabetes mellitus (%) 7 (12.3%) 6 (10.3%) 0.777 User of anticoagulant medication (%) 10 (17.6%) 17 (29.3%) 0.248 Repair technique Ellipse ROM flap Keystone flap Rotation A-T flap Other transposition flap Other advancement flap Split thickness (halo) graft 0 31 (54.4%) 6 (10.5%) 6 (10.5%) 6 (10.5%) 2 (3.5%) 1 (1.8%) 5 (8.8%) 1 (1.7%)a 28 (48.3%) 7 (12.1%) 7 (12.1%) 8 (13.8%) 3 (5.2%) 1 (1.7%) 3 (5.2%) 0.970 Median average diameter defect size 20.0 20.0 0.335 (IQR), mm (16, 22) (16, 24.5)b BCC = basal cell carcinoma; IQR = interquartile range; SCC = squamous cell carcino- ma; SD = standard deviation; ROM = reducing opposed multilobed flap. a Patient receiving ellipse repair technique was removed from follow-up. b Based on 57 patients. TABLE 3. Sensitivity Analysis of Surgical Site Infection by Intention to Treat Control Group Intervention Group Difference: Control Minus Intervention Two-Sided 95% CI P Value Participants who completed follow-up 17/55 (30.9%) 14/55 (25.5%) 5.4% −11.4, 22.2 0.525 Sensitivity analysis Assuming all lost to follow- up did not develop SSI 17/57 (29.8%) 14/58 (24.1%) 5.7% −10.5, 21.9 0.492 Assuming all lost to follow- up did develop SSI 19/57 (33.3%) 17/58 (29.3%) 4.0% −12.9, 20.9 0.642 CI = confidence interval; SSI = surgical site infection. Research | Dermatol Pract Concept 2019;9(1):8 33 rate of 12.5% in the intervention group, although this study was underpowered to produce statistical significance [17]. Our study differed in that we included only below-knee exci- sions, which are at higher risk than the entire lower limb, and flap and graft surgery, which are at higher infection risk than ellipse excisions. Other studies examining antibiotic prophylaxis for surgi- cal sites elsewhere have also demonstrated the effectiveness of antibiotic prophylaxis, in contrast with our study. Two RCTs—one involving flap and graft repairs in a dermatologi- cal surgery setting [31] and the other involving ear and nose only [32]—confirmed that single-dose oral antibiotic prophy- laxis prevented SSI. Although a further RCT involving graft repairs on the nose was underpowered to show a reduction in SSI, graft survival was better for those randomized to anti- biotic prophylaxis [33]. A recent meta-analysis of 12 RCTs studying antibiotic prophylaxis in dermatological surgery demonstrated that preoperative antibiotic prophylaxis was effective in preventing SSI and furthermore that single antibi- otic use is of adequate efficacy and safety for preventing SSI [34]. It should be noted, however, that only 2 of these RCTs investigated oral antibiotic prophylaxis, with intravenous antibiotics investigated in the remaining 10 studies [34]. In 4 participants with SSI, the infection did not respond to cephalexin. Swabs in 2 cases (1 intervention, 1 control) isolated organisms not sensitive to cephalexin (Pseudomonas aeruginosa in one case, Enterobacter cloacae in the other). In a further 2 participants (1 control, 1 intervention), 2 organisms were isolated on microscopy and culture: the S aureus found in each case was sensitive to cephalexin but the second organism isolated (P aeruginosa in one case and Streptococcus C in the other) was not sensitive to cephalexin. Ciprofloxacin was introduced for each SSI not responding to cephalexin, in accordance with swab sensitivity results. Discussion The results of this trial did not show any clinically or statis- tically significant reduction in the rate of SSI from a single dose of cephalexin administered 40-60 minutes before skin incision. There was no increase in adverse outcomes related to antibiotic administration in the intervention group. These results contrast to the only previously identified study examining the effect of antibiotic prophylaxis in lower limb ellipse skin excisions, which showed a reduction in the incidence of SSI from a similar baseline rate of 35.7% to a TABLE 4. Comparison of Analgesia Requirements, Adverse Symptoms, Follow-up Treat- ment, and Swab Results for Intervention and Control Study Participants Characteristic Control (n = 55) Intervention (n = 55) P Value Surgical site infection 17 (30.9%) 14 (25.5%) 0.525 Analgesia requirements (%) None Strongest paracetamol Strongest Panadeinea Strongest Panadeine Fortea Strongest Endoneb 45 (81.8%) 8 (14.5%) 0 2 (3.6%) 0 43 (78.2%) 11 (20.0%) 0 0 1 (1.8%) 0.329 Adverse symptoms (%) Nausea following ingestion of study capsules Diarrhea following ingestion of study capsules 0 0 1 (1.8%) 0 1.0 1.0 Antibiotics started (%) None Cephalexin Dicloxacillin Unknown antibiotic started by nonpractice doctor 38 (69.1%) 16 (29.1%) 0 1 (1.8%) 41 (74.5%) 12 (21.8%) 1 (1.8%) 1 (1.8%) 0.753 Swab result (%) (n = 21) S aureus sensitive to cephalexin S aureus sensitive to cephalexin + 2nd organism not sensitive to cephalexin Other organism sensitive to cephalexin Other organism not sensitive to cephalexin Normal skin flora 8 (%) 1 (%) 0 1 (%) 2 (%) 4 (%) 1 (%) 1 (%) 1 (%) 2 (%) 0.879 a Paracetamol and codeine. b Oxycodone. 34 Research | Dermatol Pract Concept 2019;9(1):8 Conclusions Despite confirmation that the majority of complex below- knee closure SSIs responded effectively to oral cephalexin postoperatively, we were unable to demonstrate that a single preoperative 2-g dose of cephalexin could prevent SSI from occurring. Infection rates for below-knee surgery are unacceptably high, even in temperate climates. As antibiotic prophylaxis has been shown to be helpful for other dermatological high- risk areas, further research experimenting with different antibiotic prophylactic regimens is worthwhile. Acknowledgments We thank trial recruiting doctors (Drs. Alan Poggio, Jeremy Hudson, Robert Teunisse, Lauren Barcley, Sandra Steele, Michael Khong, and Abid Vitani), the trial clinical nurse (Lyndie Terry), and data collection personnel (Lorraine Fisher, Lynne Kelly, and Angela Byers). We also thank Dr Pranav Divakarum and Dr Leanne Hall for their help in preparing the manuscript. References 1. Haas AF, Grekin RC. Antibiotic prophylaxis in dermatologic surgery. J Am Acad Dermatol. 1995;32(2 Pt 1):155-176. 2. Dettenkofer M, Wilson C, Ebner W, Norgauer J, Rüden H, Daschner FD. Surveillance of nosocomial infections in dermatol- ogy patients in a German university hospital. Br J Dermatol. 2003;149(3):620-623. 3. 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Wound care in the dermatology office: where are we in 2011? J Am Acad Dermatol. 2011;64(3 Suppl):S1-S7. 10. Penington A. Ulceration and antihypertensive use are risk factors for infection after skin lesion excision. ANZ J Surg. 2010;80(9):642-645. 11. Dixon AJ, Dixon MP, Dixon JB. Prospective study of skin surgery in patients with and without known diabetes. Dermatol Surg. 2009;35(7):1035-1040. The recruited cases for this study were at particularly high infection risks, and we postulate that this was the reason for the failure of antibiotic prophylaxis in our study. First, the anatomical site studied was below the knee, which has been shown to have higher risk of infection than other ana- tomical sites [13,16]. Second, only flap and graft surgery was included, which is known to be of higher SSI risk than simpler surgical techniques [20]. 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The strengths of our study were a blind, randomized design with placebo control, a standardized protocol for excision and follow-up, as well as the collection of a large amount of demographic, medical, and excision-related data for comparison of groups. We must, however, acknowledge some limitations to our study. Infection may have been underreported, with partici- pants presenting to doctors outside the study practice after surgery. Various characteristics influence SSI, and although information on as many variables as possible was recorded, it is difficult to ensure that all possible variables are comparable at baseline. Despite the intention to take swabs for micros- copy culture and sensitivity for all cases of clinical SSI, only 21 swabs were taken in 31 suspected SSI cases. There may also be limitations to generalizing our find- ings. 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